Acetazolamide

For research use only.

Catalog No.S4506 Synonyms: Diamox

1 publication

Acetazolamide Chemical Structure

CAS No. 59-66-5

Acetazolamide (Diamox), a potent carbonic anhydrase (CA) inhibitor, is commonly used in clinical practice as an immediate and readily available option for acute reduction of intraocular pressure(IOP).

Selleck's Acetazolamide has been cited by 1 publication

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Biological Activity

Description Acetazolamide (Diamox), a potent carbonic anhydrase (CA) inhibitor, is commonly used in clinical practice as an immediate and readily available option for acute reduction of intraocular pressure(IOP).
Targets
Carbonic anhydrase [4]
10 nM
In vitro

acetazolamide reduces invasiveness of cancer cells in vitro[3].

Assay
Methods Test Index PMID
Growth inhibition assay
Cell viability ; 

PubMed: 28235409     


Graphic and tabulated evidence for the dose response. Doses were AZ(0–160 μM) for 48 h treatment of NSC6539, NSC6562, SH-SY5Y, SK-N-SH and SK-N-BE(2) cells compared to the untreated group.

28235409
In vivo Acetazolamide reduces inflammation-induced heat hyperalgesia, probably acting both peripherally and centrally[1]. Acetazolamide shows significant inhibitory effect on angiogenesis in CAM and endothelial cell proliferation[2]. Acetazolamide is clinically used to reduce intraocular pressure in glaucoma, to correct metabolic alkalosis, and to manage cerebral edema. Its treatment may also be beneficial as an adjunct to cancer chemotherapy, because it can produce additive tumor growth delays with anticancer drugs in vivo[3].

Protocol

Cell Research:

[3]

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  • Cell lines: renal carcinoma cell lines(Caki-1, Caki-2, ACHN, and A-498)
  • Concentrations: 10 μM
  • Incubation Time: 48 h
  • Method:

    The invasion assay is performed in a Biocoat Matrigel invasion chamber. Cells (n = 1.8 × 105) are added to the chamber and cultured for 48 h in the presence or absence of acetazolamide, which was administered twice during the 48-h period: the first dose at the beginning of the assay and the second after 24 h. After growing for 48 h, noninvasive cells are removed from the upper surface of the membrane with a cotton swab. The invasive cells on the lower surface of the membrane are fixed with 4% (vol/vol) neutral-buffered formaldehyde, stained with toluidine blue, and counted in 20 separate areas with a Leitz Diaplan microscope.


    (Only for Reference)
Animal Research:

[1]

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  • Animal Models: Male Sprague-Dawley rats
  • Dosages: 10, 100, 200 mg/kg
  • Administration: i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 44 mg/mL (197.97 mM)
Water Insoluble
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 222.25
Formula

C4H6N4O3S2

CAS No. 59-66-5
Storage powder
in solvent
Synonyms Diamox
Smiles CC(=O)NC1=NN=C(S1)S(=O)(=O)N

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04679389 Recruiting Drug: Placebo|Drug: Acetazolamide Pmm2-CDG|CDG1A Mayo Clinic|Seattle Children''s Hospital|Children''s Hospital of Philadelphia March 17 2021 Phase 2|Phase 3
NCT03720288 Unknown status Drug: Acetazolamide|Drug: Placebo Heart Failure|Acute Heart Failure University of Sao Paulo General Hospital October 1 2018 Phase 3
NCT03536520 Active not recruiting Drug: ACETAZOLAMIDE oral capsule|Drug: Placebo oral capsule Altitude Hypoxia University of Zurich|National Center of Cardiology and Internal Medicine named after academician M.Mirrakhimov June 1 2018 Phase 4
NCT03540914 Active not recruiting Drug: ACETAZOLAMIDE oral capsule|Drug: Placebo oral capsule Altitude Hypoxia University of Zurich|National Center of Cardiology and Internal Medicine named after academician M.Mirrakhimov June 1 2018 Phase 4
NCT03540901 Active not recruiting Drug: ACETAZOLAMIDE oral capsule|Drug: Placebo oral capsule Chronic Obstructive Pulmonary Disease University of Zurich|National Center of Cardiology and Internal Medicine named after academician M.Mirrakhimov May 22 2018 Phase 4

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Carbonic Anhydrase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID