research use only

Indisulam (E7070) Carbonic Anhydrase inhibitor

Name: Indisulam (E7070)
Brand: Selleck Chemicals
SKU: S9742
Availability: InStock
Rating: 5 (3 reviews)

Cat.No.S9742

Indisulam (E7070), a sulfonamide anticancer agent, is a potent carbonic anhydrase (CA) inhibitor that inhibits cytosolic isozymes I and II, and transmembrane, tumor-associated isozyme IX. Indisulam suppresses the expression of cyclin E and phosphorylation of CDK2, both of which are essential for the G1 to S transition.

Chemical Structure

Molecular Weight: 385.85

Jump to

Quality Control

Batch: S974201 DMSO]77 mg/mL]false]Ethanol]10 mg/mL]false]Water]Insoluble]false Purity: 99.85%

Cited in Nature Medicine for its top-tier quality
COA
NMR
SDS
Datasheet

99.85

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other Carbonic Anhydrase Products	U-104 Benzenesulfonamide CA9 Antibody [J19A24] Tioxolone Girentuximab (Anti-CA9 / Carbonic anhydrase 9) Carbonic anhydrase 2/CA2 Antibody [C4P17] Carbonic Anhydrase 1/CA1 Antibody [H20K14] 2-Aminobenzenesulfonamide

Solubility

In vitro
Batch:

DMSO : 77 mg/mL (199.55 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 10 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.850mg/ml (9.98mM)	Taking the 1 mL working solution as an example, add 50 μL of 77 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.640mg/ml (1.66mM)	Taking the 1 mL working solution as an example, add 50 μL of 12.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	385.85	Formula	C₁₄H₁₂ClN₃O₄S₂	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	165668-41-7	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	E7070			Smiles	N[S](=O)(=O)C1=CC=C(C=C1)[S](=O)(=O)NC2=C3[NH]C=C(Cl)C3=CC=C2

Mechanism of Action

Targets/IC₅₀/Ki	CA cyclin E CDK2
In vitro	Following treatment with this compound, the cell cycle progression of P388 murine leukaemia cells is disturbed in the G1 phase. The cell-killing effect on human colon cancer HCT116 cells is found to be time-dependent. In the panel of 42 human tumour cell lines, it shows an antitumour spectrum that is distinct from those of other anticancer drugs used in clinic.
In vivo	Animal tests using human tumour xenograft models demonstrates that this compound can cause not only tumour growth suppression, but also tumour regression in three of five colorectal and two of two lung cancers. In the HCT116 xenograft model, it is shown to be superior to 5-FU, MMC and CPT-11. Furthermore, complete regression of advanced LX-1 tumours is observed in 80% of E7070-treated mice.
References	[1] https://pubmed.ncbi.nlm.nih.gov/14684331/ [2] https://pubmed.ncbi.nlm.nih.gov/11677118/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT00165594	Terminated	Gastric Cancer	Eisai Co. Ltd.\|Eisai Inc.	February 2005	Phase 1\|Phase 2
NCT00165854	Completed	Colorectal Cancer (CRC)	Eisai Limited\|Eisai Inc.	March 2003	Phase 2

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):