Vorinostat (SAHA)

Catalog No.S1047 Synonyms: MK0683, Suberoylanilide hydroxamic acid, Zolinza

For research use only.

Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683, Zolinza) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.

Vorinostat (SAHA) Chemical Structure

CAS No. 149647-78-9

Selleck's Vorinostat (SAHA) has been cited by 485 publications

Purity & Quality Control

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Biological Activity

Description Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683, Zolinza) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.
Targets
HDAC [1]
(Cell-free assay)
~10 nM
In vitro

Vorinostat inhibits the activities of HDAC1 and HDAC3 with IC50 of 10 nM and 20 nM, respectively. Vorinostat also results in a marked hyperacetylation of histone H4. [1] Vorinostat inhibits the growth of three prostate cancer cell lines LNCaP, PC-3 and TSU-Pr1 at micromolar concentrations (2.5-7.5 μM), and induces dose-dependent cell death in LNCaP cells. [2] Vorinostat treatment in MCF-7 cells inhibits cell proliferation at an IC50 of 0.75 μM resulting in the accumulation of cells in the G1 and G2-M phase of the cell cycle. Vorinostat also induces differentiation in the estrogen receptor-negative cell line SKBr-3 and the retinoblastoma-negative cell line MDA-468. [3] Vorinostat treatment at 1 μM for 8 hours or more is sufficient to irreversibly induce apoptosis of human multiple myeloma (MM) cells. The gene expression profiles of Vorinostat treated MM cells are not hallmarked by global transcriptional activation, but by coordinated transcriptional changes of specific functional groups of genes such as cytokine-induced proliferative/survival signaling cascades, oncogenes-tumor suppressor genes, regulators of apoptosis, DNA synthesis-repair and cell cycle, and proteasome-ubiquitin function. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H1299 MUXLbY5ie2ViQYPzZZk> MkDyNlAhdWmw M4T0[GROW09? NFn5THNKdmirYnn0c5J6KGGldHn2bZR6KGGpYXnud5QhUESDQzDlcpp6dWVid3n0bEBKSzVyIH;mJFAvOTl2IN88US=> NGLWcXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEWyNVQzOid-MUS1NlE1OjJ:L3G+
HT1080 MnmyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3z1TVczKGh? M2eyS2ROW09? M{XRe2lEPTB;Mj60JO69VQ>? NYfzN5B2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUKyO|AyPzVpPkGyNlcxOTd3PD;hQi=>
K562 MV3LbY5ie2ViQYPzZZk> M1\4bVYhcA>? NX\zdno1TE2VTx?= NH73O4xKdmirYnn0bY9vKG:oIHj1cYFvKGirc4TvcoUh\GWjY3X0fYxie2ViKH3pfJR2emVib3[gTGRCSzFiYX7kJGhFSUN{KTD3bZRpKEmFNUCgc4YhOC5yMTFOwG0> MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjRzOUO4NEc,OTJ2MUmzPFA9N2F-
A20 MXjLbY5ie2ViQYPzZZk> NVv0Rmd2OSCq M2\vbWROW09? NXPheWV5[W62aT3ISGFEOSCjY4Tpeol1gSC5aYToJGlEPTBib3[gNE4yOTJizszN M17IUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOU[wOFg6Lz5zMUm2NFQ5QTxxYU6=
Friend leukemic cell MlLPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XRTlUxKM7:TR?= NIixWYM4OiCq NGnVXZRFVVOR M4DZZWlEPTB;MD65PUDPxE1? NHv2bJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkGwPVkyOyd-MUKxNFk6OTN:L3G+
H1299 M{PvUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUK3NkBp Mo\WSG1UVw>? NHrmdIFKSzVyPUeuNlQh|ryP Ml:5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR3MkG0NlIoRjF2NUKxOFIzRC:jPh?=
HeLa MnrNT4lv[XOnIFHzd4F6 M4rBPFMxKG2rbh?= M1WydmlvcGmkaYTvdpkh[2:wY3XueJJifGmxbjDh[4FqdnO2IHjpd5RwdmViZHXhZ4V1gWyjc3Wge4l1cCCLQ{WwJI9nKDBwMkig{txO MoDlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV4MEO5OFkoRjF3NkCzPVQ6RC:jPh?=
LOX-IMVI NY\6XmloT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlvlOFghcA>? M1jFXmlEPTB;MT6zJO69VQ>? M2j1UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{G1OFcxLz5zNUexOVQ4ODxxYU6=
NCI-H226 NV;PcYhbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX20PEBp M1vyOGlEPTB;Mj62JO69VQ>? NXLRSJdjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3NVU1PzBpPkG1O|E2PDdyPD;hQi=>
RXF-631L NGjrUGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkXBOFghcA>? NWnL[nlvUUN3ME2yJO69VQ>? M1;we|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{G1OFcxLz5zNUexOVQ4ODxxYU6=
SNB-78 NWKxUHRnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1rIW|Q5KGh? NULyS4VzUUN3ME2xOkDPxE1? NHXhelM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUexOVQ4OCd-MUW3NVU1PzB:L3G+
St-4 NYnqUmNKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYrpW3NuPDhiaB?= MXnJR|UxRTVwMjFOwG0> MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTdzNUS3NEc,OTV5MUW0O|A9N2F-
ARP1 M4PPNGZ2dmO2aX;uJGF{e2G7 M4L1R|Ih|ryP MUW2JIg> NEnh[3RKdmS3Y4Tpc44hd2ZiUEKxW2FHOSCpZX7lJIV5eHKnc4Ppc44> M1zOeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2N{O0PFA3Lz5zNEezOFgxPjxxYU6=
CEM M2X5TGtqdmG|ZTDBd5NigQ>? NWjoU3ZWOSCq MULEUXNQ NGXSXpBKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuN|Mh|ryP MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjR{MEC2OEc,OTZ2MkCwOlQ9N2F-
HCT15 Mo[5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3kR5J{PDhiaB?= NF7IbWhIUTVyPUCuPFIh|ryP M3Hzc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4N{KzNlI4Lz5zNkeyN|IzPzxxYU6=
NCI-H23 NFH0U5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF7QZ3A1QCCq MX\HTVUxRTBwOUKg{txO M{K1RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4N{KzNlI4Lz5zNkeyN|IzPzxxYU6=
GM15850 NVS0XWdtU2mwYYPlJGF{e2G7 NHnENGYzNjVizszN NGDp[lk6PiCq MmnnSG1UVw>? MXXJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKMzDsfZNqdmViMUSgZYNmfHmuYYTpc44hd2ZiRmjOJIdmdmV? NFnPV289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmyNVM3Pyd-MU[5NlE{Pjd:L3G+
GM15850 NYPoU5RPU2mwYYPlJGF{e2G7 NXvxZoQxOi53IN88US=> MUW5OkBp M{ntW2ROW09? MnvtTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViSESgcJl{cW6nIEWgZYNmfHmuYYTpc44hd2ZiRmjOJIdmdmV? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl{MUO2O{c,OTZ7MkGzOlc9N2F-
GM15850 NXXDN|V4U2mwYYPlJGF{e2G7 NXTGOXBVOi53IN88US=> M1;ZOFk3KGh? M4rwe2ROW09? MYTJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKNDDsfZNqdmViMUKgZYNmfHmuYYTpc44hd2ZiRmjOJIdmdmV? NFTVTZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmyNVM3Pyd-MU[5NlE{Pjd:L3G+
GM15850 NHnEUXVMcW6jc3WgRZN{[Xl? NWThVY9tOi53IN88US=> NX3E[3lHQTZiaB?= NXjT[lMyTE2VTx?= MYPJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKMzDsfZNqdmViOTDhZ4V1gWyjdHnvckBw\iCIWF6g[4Vv\Q>? MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl{MUO2O{c,OTZ7MkGzOlc9N2F-
GM15850 NGLSNI9McW6jc3WgRZN{[Xl? M4PGbFIvPSEQvF2= NISyeHo6PiCq M33Wb2ROW09? NXr4enVYUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFQhdHm|aX7lJFgh[WOndInsZZRqd25ib3[gSnhPKGenbnW= M4HyOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUKxN|Y4Lz5zNkmyNVM3PzxxYU6=
GM15850 NVL1e|JwU2mwYYPlJGF{e2G7 MViyMlUh|ryP MnPNPVYhcA>? NITDSIVFVVOR MlPSTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViSESgcJl{cW6nIEG2JIFk\XS7bHH0bY9vKG:oIF\YUkBo\W6n M{XJ[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUKxN|Y4Lz5zNkmyNVM3PzxxYU6=
U937 NYi2[m53TnWwY4Tpc44hSXO|YYm= MkXCOUDPxE1? NWnpPHRlOjRiaB?= NWr4ToJ5TE2VTx?= MUDD[YxtKGO7Y3zlJIFzemW|dDDifUBi[2O3bYXsZZRqd25iYYSgV{9IOi:PIIDoZZNm NIKxeVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{CwOFcyQCd-MUewNFQ4OTh:L3G+
H661 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVm0PEBp NUXuWZpVUUN3ME2xJO69VQ>? NYrFV3JXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUexOVcxODlpPkG3NVU4ODB7PD;hQi=>
U937 MX;GeY5kfGmxbjDBd5NigQ>? MmLqOUDPxE1? M3KyVFI1KGh? MYDEUXNQ NVzVOIs3UW6lcnXhd4UhcW5iaHnzeI9v\SCKMzDhZ4V1gWyjdHnvci=> NHjVfpc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S0O|c2OCd-MUe0OFc4PTB:L3G+
U937 M2TI[mZ2dmO2aX;uJGF{e2G7 MWOxJO69VQ>? MYCyOEBp NEjSPJdFVVOR MoGxSYZn\WO2IH;uJIFteGijIIT1ZpVtcW5iYXPleJlt[XSrb36= MlrXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2NEe3OVAoRjF5NES3O|UxRC:jPh?=
RAW264.7 MVLGeY5kfGmxbjDBd5NigQ>? M2KyXlExKM7:TR?= MmGzNlRp NUWwfVdSf2G2ZYK= NWTlfWFXUW6qaXLpeIlwdiCxZjDMVHMue3SrbYXsZZRm\CCWTl\hcJBp[SCycn;keYN1cW:wIIfpeIghUUN3MDDv[kAxNjd4IN88US=> MmfzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2N{e1NVgoRjF5NEe3OVE5RC:jPh?=
RAW264.7 NXHUN29bTnWwY4Tpc44hSXO|YYm= MWmxNEDPxE1? NEn5cHQzPGh? NVnRWppCf2G2ZYK= MVXJcohq[mm2aX;uJI9nKEySUz3zeIlufWyjdHXkJG5QKHC{b3T1Z5Rqd25id3n0bEBKSzVyIH;mJFEvQDhizszN MlrzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2N{e1NVgoRjF5NEe3OVE5RC:jPh?=
SC9 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH\2U5A1QCCq NFn3bmZKSzVyPUCuOlA3KM7:TR?= NVLjcZEyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NFczOTlpPkG3OVA4OjF7PD;hQi=>
ST14A MYnGeY5kfGmxbjDBd5NigQ>? NVzPWHB1PSCwTR?= MWq3NkBp MojMTY5kemWjc3WgbY4hWkWVVD;OVnNHKG2UTlGg[ZhxemW|c3nvci=> NFjHeI89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W2OVk6Oyd-MUe1OlU6QTN:L3G+
ST14A NV;EN|ZSTnWwY4Tpc44hSXO|YYm= M1HrOlUhdk1? NVzVR2NnPzJiaB?= Moq0TY5kemWjc3WgbY4hTTKIMTDtVm5CKGW6cILld5Nqd25? M{TQNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NU[1PVk{Lz5zN{W2OVk6OzxxYU6=
PANC1 M1GxRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHzTmMyKM7:TR?= M3XaSGlEPTB;MD60OUDPxE1? M1XsW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUi4O|Q1Lz5zN{W4PFc1PDxxYU6=
PANC1 M3TjOmZ2dmO2aX;uJGF{e2G7 MkLBNUDPxE1? Mke0NlQhcA>? MmHhTY5kemWjc3WgbY4heDJzV1HGNU9EUVBzIHflcoUh\XiycnXzd4lwdg>? MnjBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OEi3OFQoRjF5NUi4O|Q1RC:jPh?=
PANC1 Mkj4SpVv[3Srb36gRZN{[Xl? MX[xJO69VQ>? M{SwXFI1KGh? M3H4Umlv[3KnYYPlJIlvKHB{N3vpdFEh\2WwZTDlfJBz\XO|aX;u M{P5eFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUi4O|Q1Lz5zN{W4PFc1PDxxYU6=
PT45 M1TtUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWDBd41NUUN3ME20JO69VQ>? NXzCTJl6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PFg4PDRpPkG3OVg5PzR2PD;hQi=>
ACHN NHfJPIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXzUUYp2OjFiaB?= MlfuS2k2OD1yLk[1JO69VQ>? MmfXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NkK2NFYoRjF5Nk[yOlA3RC:jPh?=
PC3 MlLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;wSIlUOjFiaB?= NXPNXXNvT0l3ME2wMlcyKM7:TR?= NUjScIdDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2OlI3ODZpPkG3OlYzPjB4PD;hQi=>
A431 M2LoO2N6fG:2b4jpZ{BCe3OjeR?= NEfabYI4OiCq Ml3GTWM2OD12LkKg{txO M3nEdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkmxO|Y{Lz5zN{[5NVc3OzxxYU6=
Cal27 MlTNR5l1d3SxeHnjJGF{e2G7 NUW3botVPzJiaB?= MlfFTWM2OD1|LkKg{txO NFrtco49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[5NVc3Oyd-MUe2PVE4PjN:L3G+
CCRF-CEM MonvR5l1d3SxeHnjJGF{e2G7 M3[5NVczKGh? MlG5TWM2OD1yLkig{txO MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ7MUe2N{c,OTd4OUG3OlM9N2F-
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Hec-1-A MnjzR5l1d3SxeHnjJGF{e2G7 MYm3NkBp MkK4TWM2OD1zIN88US=> Mm\JQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4OUG3OlMoRjF5NkmxO|Y{RC:jPh?=
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CFBE41o- NYe5eZlmU2mwYYPlJGF{e2G7 NGq1U4Q2KM7:TR?= MViyOEBp NYHEcm1qTE2VTx?= MWnJcohq[mm2aX;uJI9nKEiGQVO3JIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIIP0ZYJqdGm2eTDv[kBH\GWudHG1NFghS0[WUjDwdo91\Wmw NFjZfow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm2Olc5QSd-MUm5OlY4QDl:L3G+
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Primary bronchial epithelial cell NIjXUGhMcW6jc3WgRZN{[Xl? M2njOVUh|ryP MoXnNlQhcA>? M{PVNmROW09? NGjSUoFKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJI12fGGwdDDG[IVtfGF3MEigR2ZVWiCycn;0[YlvKGGyaXPhcEB{fXKoYXPlJIxw[2GuaYrheIlwdg>? NF6zSmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm2Olc5QSd-MUm5OlY4QDl:L3G+
Primary bronchial epithelial cell M2nXZ2tqdmG|ZTDBd5NigQ>? NGPmbJcyKM7:TR?= NWSzWGFPQTZiaB?= NWnpfHkyTE2VTx?= M2rn[GlvcGmkaYTpc44hd2ZiSFTBR|ch[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gd4hwenRiY3nyZ5VqfCCldYLy[Y51ew>? Mn\IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Nk[3PFkoRjF7OU[2O|g6RC:jPh?=
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CAL27 NWDOfWl2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITEUYw4OiCq NGPCcplKSzVyPUOuO|Ih|ryP MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{e3PEc,OjBzNEO3O|g9N2F-
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HCC827 NGLveolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnDPG44OiCq NFnIb4NKSzVyPUGuPEDPxE1? MlXlQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO3O|goRjJyMUSzO|c5RC:jPh?=
LNCAP M2L0[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYq3NkBp NGHBSGlKSzVyPUCuOVgh|ryP NIT4NFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N|c4QCd-MkCxOFM4Pzh:L3G+
NCI-H2122 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEXJbFc4OiCq M1e1NGlEPTB;Nz61JO69VQ>? MkHrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO3O|goRjJyMUSzO|c5RC:jPh?=
NCI-H358 NYP3N2pKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYHrT4N1PzJiaB?= MVXJR|UxRTJwNTFOwG0> NXe2W|N1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM4PzhpPkKwNVQ{Pzd6PD;hQi=>
SCC15 MnnxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGHlblg4OiCq NXjle2V7UUN3ME2yMlQ6KM7:TR?= NIOwe5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N|c4QCd-MkCxOFM4Pzh:L3G+
SCC25 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF[ySm04OiCq MYrJR|UxRTFwOUOg{txO NYezcGcyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM4PzhpPkKwNVQ{Pzd6PD;hQi=>
SKHEP1 Mk\lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFnmUVI4OiCq MlWwTWM2OD1|LkS2JO69VQ>? NYPMNnU2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM4PzhpPkKwNVQ{Pzd6PD;hQi=>
BJ Mln4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mmr5NVAxKM7:TR?= NXKzWHBzPDhiaB?= MmTLSG1UVw>? M2\1NWdzd3e2aDDpcohq[mm2aX;uJIF1KDBwMEWgeI8hOTByIIXN MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
MUF MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3jZNFExOCEQvF2= MXW0PEBp Mn70SG1UVw>? NV7w[4JMT3Kxd4ToJIlvcGmkaYTpc44h[XRiMD6wOUB1dyBzMECgeW0> NGnXR4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N|g1OCd-MkCxOFM5PDB:L3G+
Caco-2 M2DrRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2LHXVExOCEQvF2= MWC0PIg> M170e2ROW09? MVLJR|UxRThwMTFOwG0> MlzXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
Caco-2 NWPxc2JoU2mwYYPlJGF{e2G7 M3rpVVUh|ryP NEfhZYM1KGh? M1fxU2ROW09? MWjJcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDobZN1d26nIHHj[ZR6dGG2aX;uJGlEPTBib3[gNE4xOzdizszN NHvO[GE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N|g1OCd-MkCxOFM5PDB:L3G+
Caco-2 MlnvT4lv[XOnIFHzd4F6 M2Xhb|AvOzF4IH7NJJRwKDNzLk[g{txO NYqz[VlTPCCq MUfEUXNQ MlO5SZghfmm4bzDpcohq[mm2aX;uJI9nKGi3bXHuJGhFSUNzIIfpeIghUUN3MDDv[kAxNjFzOTFOwG0> NYjNcoNmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
Caco-2 MWTLbY5ie2ViQYPzZZk> M1O2cFAvOzF4IH7NJJRwKDNzLk[g{txO MmfsOEBp NHjIZopFVVOR M4LHUmV5KH[rdn:gbY5pcWKrdHnvckBw\iCqdX3hckBJTEGFMzD3bZRpKEmFNUCgc4YhOC5zNEeg{txO NX\hdWc3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
Caco-2 MVvLbY5ie2ViQYPzZZk> NFHTS5E3NjN{IH7NJJRwKDZ|MjFOwG0> NXjyO|FtPCCq NFm3OWhFVVOR NVL6[GVxTXhidnn2c{BqdmirYnn0bY9vKG:oIHj1cYFvKEiGQVO1JJdqfGhiSVO1NEBw\iBzMj62JO69VQ>? MnrYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
Caco-2 NVmxNZJFU2mwYYPlJGF{e2G7 NYrrWHF1Pi5|MjDuUUB1dyB4M{Kg{txO NV[yTms3PCCq M3nKZ2ROW09? NEG2cppGgCC4aY\vJIlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{ege4l1cCCLQ{WwJI9nKDRzIN88US=> MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
Caco-2 NYLUb2dLU2mwYYPlJGF{e2G7 NFnoW|ExNjNzNjDuUUB1dyB|MT62JO69VQ>? MYK0JIg> M3zFfmROW09? M3HiUWV5KH[rdn:gbY5pcWKrdHnvckBw\iCqdX3hckBJTEGFNh?= MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
HepG2 NGnSW29McW6jc3WgRZN{[Xl? M4LDelUh|ryP M1\VUVZp NUfmemx6TE2VTx?= MX7JcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDobZN1d26nIHHj[ZR6dGG2aX;u MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
HepG2 MXzLbY5ie2ViQYPzZZk> M13nN|Uh|ryP MXG2bC=> NYXTVmpsTE2VTx?= M2nvSmlv\HWldHnvckBw\iCKRFHDMY1m\GmjdHXkJIhqe3SxbnWgbJlx\XKjY3X0fYxifGmxbh?= M1y1SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzPFQxLz5{MEG0N|g1ODxxYU6=
HepG2 NYLQ[pJyU2mwYYPlJGF{e2G7 M4jS[VUh|ryP MUi2bC=> Mli3SG1UVw>? NH75bZdKdmS3Y4Tpc44hd2ZiSFTBR{1u\WSrYYTl[EBidHCqYT30eYJ2dGmwIHHj[ZR6dGG2aX;u MlPqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
HepG2 NXL1fI5IU2mwYYPlJGF{e2G7 MljEOUDPxE1? NWX6SVRNPmh? M1K2ZmROW09? NHXMT|dKdmS3Y4Tpc44hd2ZiSFTBR{1u\WSrYYTl[EBJOy:KNDDobZN1d26nIHHj[ZR6dGG2aX;u NYrHdVVSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
COLO205 NVPXclFHU2mwYYPlJGF{e2G7 NUHNc2JUOTBizszN M13zflI1KGh? MWHEUXNQ NXTSRYxZUW6qaXLpeIlwdiCxZjDISGFEOSCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhcGm|dH;u[UBJOyCjY3X0fYxifGmxbjD3bZRpKEmFNUCgc4YhOi5{IN88US=> M2jkZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEWxN|c5Lz5{MES1NVM4QDxxYU6=
SCC4 NXv3fG41U2mwYYPlJGF{e2G7 MV2xJO69VQ>? NH\2U4czPGh? M2qx[mlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjD0eYJ2dGmwIHHj[ZR6dGG2aX;u NFjuNnc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES1NlIzPSd-MkC0OVIzOjV:L3G+
SCC4 NX7FXIJIU2mwYYPlJGF{e2G7 NHrP[3EyKM7:TR?= NGHYOpkzPGh? Mo\LTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViYXPleJlt[XSrb36= NHHKd249[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES1NlIzPSd-MkC0OVIzOjV:L3G+
SCC4 MY\GeY5kfGmxbjDBd5NigQ>? NV;hVWF[OSEQvF2= NHrtXlY2KG2rbh?= MlqwRYdwdmm|dDDhZ5Rqfmm2eTDheEBXTFJiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKEO\UEK0JIdmdmViZYjwdoV{e2mxbh?= NVS3b44yRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0OVIzOjVpPkKwOFUzOjJ3PD;hQi=>
NB4 M1zz[WZ2dmO2aX;uJGF{e2G7 NIXpVIk2KM7:TR?= NG\6TZIzPCCq Ml;ySG1UVw>? M3PTbGNmdGxiY4njcIUh[XK{ZYP0JIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHHjZ5VufWyjdHnvckBifCCycnWtS|EheGijc3W= MmCxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2OUG0OFAoRjJyNEmxOFQxRC:jPh?=
NB4 MkjIT4lv[XOnIFHzd4F6 MVK1JO69VQ>? M{jlbVI1KGh? NHzIUoRFVVOR NILGelZKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gZYNmfHmuYYTlJJR2[nWuaX6gcIV3\Wx? NFzaNlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES5NVQ1OCd-MkC0PVE1PDB:L3G+
NB4 MlnXSpVv[3Srb36gRZN{[Xl? MUi1JO69VQ>? NWHJfG1JOjRiaB?= NV7NOmxVTE2VTx?= M1HGTmlv\HWldHnvckBw\iCyMkHXRWYyN0OLUEGg[ZhxemW|c3nvci=> M1vsVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEmxOFQxLz5{MES5NVQ1ODxxYU6=
NB4 M3j0[2tqdmG|ZTDBd5NigQ>? NV3hdY82PSEQvF2= MViyOEBp MlTOSG1UVw>? NWLqemFRUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFMh[WOndInsZZRqd25? NYPFO|dMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0PVE1PDBpPkKwOFkyPDRyPD;hQi=>
NUGC3 NIe0cJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DoPWdKPTB;Mj65OEDPxE1? Mm\DQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6NUC5O|EoRjJyOEWwPVcyRC:jPh?=
A549 NWLxfJlNTnWwY4Tpc44hSXO|YYm= MUS1JO69VQ>? NYL5N4E2OyCq MneySG1UVw>? Mlr6TY5lfWO2aX;uJI9nKGG3dH;wbIFogSCrbjDoeY1idiCDNUS5JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEyFM1nJ NWjMPWsxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4OVUzODhpPkKwPFU2OjB6PD;hQi=>
HUT78 M1nHVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXFO|IhcA>? NWix[oNtTE2VTx?= NEPaTm1IUTVyPUCuN{DPxE1? M4G1fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOEi0NlA5Lz5{MEi4OFIxQDxxYU6=
U937 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXm3NkBp M{DhT2ROW09? NVPxbotCT0l3ME2wMlM6KM7:TR?= MVe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh6NEKwPEc,OjB6OESyNFg9N2F-
A431 NIG4TYpEgXSxdH;4bYMhSXO|YYm= NHj5TVM4OiCq MonSR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVQ{OSClZXzsd{BwfmW{ZYjwdoV{e2mwZzDFS2ZTKHerdHigTWM2OCCxZjC0MlIh|ryP NVv4Z5BWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3OjlpPkKxNFgxPjJ7PD;hQi=>
A549 MmXHR5l1d3SxeHnjJGF{e2G7 MkXXO|IhcA>? NULZZVd6S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCxdnXy[ZhxemW|c3nu[{BJTEGFIIfpeIghUUN3MDDv[kAyNjhizszN MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
CAL27 MXXDfZRwfG:6aXOgRZN{[Xl? MnP5O|IhcA>? MVXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDDRWwzPyClZXzsd{BwfmW{ZYjwdoV{e2mwZzDFS2ZTKHerdHigTWM2OCCxZjCzMlIh|ryP NHHqOGY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFYzQSd-MkGwPFA3Ojl:L3G+
HeLa NE\wdVFEgXSxdH;4bYMhSXO|YYm= MkTJO|IhcA>? MYrDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJI93\XKneIDy[ZN{cW6pIFjERWMhf2m2aDDJR|UxKG:oIEGuOUDPxE1? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
Sf21 NVn5e4lGU2mwYYPlJGF{e2G7 M2nOSlMhcA>? NXHUfVM4UW6qaXLpeIlwdiCxZjDGcIFoKHSjZ3fl[EBpfW2jbjDy[YNwdWKrbnHueEBJTEGFODDlfJBz\XO|ZXSge4l1cCCLQ{WwJI9nKDFwMjFOwG0> Mny0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2NlkoRjJzMEiwOlI6RC:jPh?=
SKBR3 NE\BfINEgXSxdH;4bYMhSXO|YYm= MXW3NkBp NH;aZXBEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBUU0KUMzDj[YxteyCxdnXy[ZhxemW|c3nu[{BJTVJ{IIfpeIghUUN3MDDv[kAzNjZizszN NYrjcI9URGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3OjlpPkKxNFgxPjJ7PD;hQi=>
SKOV3 M4[2d2N6fG:2b4jpZ{BCe3OjeR?= M3ziN|czKGh? NYrqNFRbS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0uRVkOgZ4VtdHNib4\ldoV5eHKnc4PpcochUEWUMjD3bZRpKEmFNUCgc4YhOi5{IN88US=> NXziNnBYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3OjlpPkKxNFgxPjJ7PD;hQi=>
COR-L23 NIPm[GdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWjkTIgxOTBizszN NI\aVJc4OiCq M{DRXWROW09? NH32WJVIUTVyPUGuNUDPxE1? M2W4RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEiwOlQ4Lz5{MUC4NFY1PzxxYU6=
DU145 NFjmRXRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIi5N2MyOCEQvF2= NHfzeHc4OiCq M3LFZ2ROW09? MX\HTVUxRTFwMEWg{txO NXPBT|F{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3PDdpPkKxNFgxPjR5PD;hQi=>
LoVo NVzNXGJST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLiTZgyOCEQvF2= NWe4PWlNPzJiaB?= MoPISG1UVw>? NX;kV4RbT0l3ME2xMlch|ryP Mlr5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2OFcoRjJzMEiwOlQ4RC:jPh?=
MCR5-SV2 NVLqc4JNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmrXNVAh|ryP NH[1ToI4OiCq Mn3ySG1UVw>? M3Lqd2dKPTB;Mj6xJO69VQ>? NIfOVoc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFY1Pyd-MkGwPFA3PDd:L3G+
HCT116 M2nVZmtqdmG|ZTDBd5NigQ>? NI\idVA{OCEQvF2= NWfLS|F7OzBiaB?= NH\JNGdFVVOR MlXsTY5pcWKrdHnvckBw\iCKRFHDOkBie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gZYxxcGFvdIXieYxqdiCjY3X0fYxifGmxbh?= NHjtTmY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFY1Pyd-MkGwPFA3PDd:L3G+
HCT116 MYnLbY5ie2ViQYPzZZk> MXKzNEDPxE1? NGPUfZQ{OCCq NHn5VFRFVVOR MXzJcohq[mm2aX;uJI9nKEiGQVOxJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDobZN1d26nIFizT|kh[WOndInsZZRqd25? Ml\YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2OFcoRjJzMEiwOlQ4RC:jPh?=
U937 NITs[I5McW6jc3WgRZN{[Xl? M3PRd|Uh|ryP NHKxPGQzPCCq MYTEUXNQ NY[wNYxzUW6qaXLpeIlwdiCxZjDobZN1d26nIHHj[ZR6dHS{YX7z[oVz[XOnIHHzd4V{e2WmIHHzJIlv[3KnYYPlJI9nKGGlZYT5cIF1cW:wIH;mJIhqe3SxbnWgTFMh[XRiS{m= MorjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OUK0PVIoRjJzMkmyOFkzRC:jPh?=
U937 MVXLbY5ie2ViQYPzZZk> NFLFRYo2KM7:TR?= MY[yOEBp NF7U[2VFVVOR NU\DPJMzUW6qaXLpeIlwdiCxZjDobZN1d26nIHHj[ZR6dHS{YX7z[oVz[XOnIHHzd4V{e2WmIHHzJIlv[3KnYYPlJI9nKGGlZYT5cIF1cW:wIH;mJIhqe3SxbnWgTFMh[XRiS{G4 NGnWeXc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5NlQ6Oid-MkGyPVI1QTJ:L3G+
U937 M4PTemtqdmG|ZTDBd5NigQ>? NVu1UZhnPSEQvF2= MkDFNlQhcA>? NILS[JFFVVOR Mo\FTY5pcWKrdHnvckBw\iCqaYP0c45mKGGlZYT5cJRz[W6|ZnXyZZNmKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIH;mJJBidi2jY3X0fYxifGWmIHjpd5RwdmViSESgcIV3\Wx? NWHDOos{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPVI1QTJpPkKxNlkzPDl{PD;hQi=>
U937 MnzET4lv[XOnIFHzd4F6 MnnoOUDPxE1? MmjZNVUhdWmw NGjzdVFFVVOR MnXXTY5pcWKrdHnvckBw\iCqaYP0c45mKGGlZYT5cJRz[W6|ZnXyZZNmKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIH;mJIhqe3SxbnWgTFMh[WOndInsZZRqd25ibHX2[Yw> M{TnfFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmyOFkzLz5{MUK5NlQ6OjxxYU6=
A2780 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWTJeZB3QTZiaB?= NVrqWolmTE2VTx?= NX\WWIluUUN3ME2xMlYzKM7:TR?= NEm0RnU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MU[zOFQ{OCd-MkG2N|Q1OzB:L3G+
COLO205 M4Xld2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGnVS2U6PiCq NF;KU4dFVVOR NEXXV5BKSzVyPUKuNVIh|ryP MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTZ|NESzNEc,OjF4M{S0N|A9N2F-
Hep3B MlfhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrGWodEPDhiaB?= MmL5SG1UVw>? MnfnS2k2OD1zLkKxJO69VQ>? NFvBPVE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUexNlE1Pid-MkG3NVIyPDZ:L3G+
GM11272 NVzPOoxWTnWwY4Tpc44hSXO|YYm= M1vyT|ExOCCwTR?= NHzCN|A4OiCq MnrpSG1UVw>? NHv4S|NT\WGldHn2ZZRqd25ib3[gUYVEeDJibYX0ZY51KGW6cILld5Nqd25iYYSgNVAhfG9iMUCwJI5O M{TzcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOESwO|E3Lz5{MUi0NFcyPjxxYU6=
CFBE41o NXXMO2JrU2mwYYPlJGF{e2G7 NW\ESoRDPSEQvF2= NXe3PGQ5TE2VTx?= MV;Ec5dvKHKnZ4XsZZRqd25ib3[gTGRCSzdiYYSgNE4zKHSxIEWgeW0> NU\Ic3UyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5PFQ6PThpPkKxPVg1QTV6PD;hQi=>
CFBE41o NH;SSY1HfW6ldHnvckBCe3OjeR?= Mn3HNUDPxE1? MV6yOEBp NFu3ZpdFVVOR Mm\LVoV{fG:{YYTpc44hd2ZiQ1\UVkBl\Wy2YV[1NFghdXW2YX70JJRz[W[oaXPrbY5oKHSxIHPlcIwhe3W{ZnHj[UBieyCpbInjc4Zwem2| NIPVSIk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm4OFk2QCd-MkG5PFQ6PTh:L3G+
DU145 NYDITXJ2U2mwYYPlJGF{e2G7 NF63cVYzNjVizszN NHH4PWgzPCCq M4O2PGROW09? M37pdmlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyC3cILl[5Vt[XSrb36gc4YheDJzd3HmNUBxem:2ZXnuJIV5eHKnc4Ppc44> NHvXTpY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK2NFE3Pid-MkKyOlAyPjZ:L3G+
DU145 MlHaT4lv[XOnIFHzd4F6 MXG1JO69VQ>? MoXWNlQhcA>? MWTEUXNQ NGLwOmZKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaHnzeI9v\SCKNDDhZ4V1gWyjdHnvci=> NIrqZ|E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK2NFE3Pid-MkKyOlAyPjZ:L3G+
DU145 NVzaWXpMU2mwYYPlJGF{e2G7 MV[1JO69VQ>? MnTDNlQhcA>? MYDEUXNQ NXezUXlVUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHjpd5RwdmViSEOgbJlx\XKjY3X0fYxifGmxbh?= NHi3WIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK2NFE3Pid-MkKyOlAyPjZ:L3G+
786-0 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX62MlIh|ryP M1nkS|czKGh? NUfxemtiTE2VTx?= M4W3S2dKPTB;Mz6yJO69VQ>? NF[zV2M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
A498 MonlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYO2MlIh|ryP NWLVdmR3PzJiaB?= MXzEUXNQ MnvyS2k2OD1zLk[g{txO MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
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NCI-H460 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1j5clYvOiEQvF2= MlnOO|IhcA>? M1Tve2ROW09? NELMfWJIUTVyPUCuO|kh|ryP M3y2R|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
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OVCAR3 MkDoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPnWGhvPi5{IN88US=> MmLRO|IhcA>? NYPv[nR7TE2VTx?= M4XGZWdKPTB;MT6zJO69VQ>? NHLq[Hk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
OVCAR4 NH[1V25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\ROk4zKM7:TR?= M4PqXlczKGh? Mn;WSG1UVw>? Mn7aS2k2OD12IN88US=> M17VV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
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OVCAR8 NUWzdmloT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NISzXVA3NjJizszN M1vxeFczKGh? NFLReGtFVVOR NF\GcXVIUTVyPUCuOUDPxE1? MkXxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
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SF268 M3r6NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MU[2MlIh|ryP NVG5b4kyPzJiaB?= NGXxboxFVVOR MoPxS2k2OD1zLk[g{txO NX35fI56RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
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Bel7402 MojvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NF\kcoM4OiCq MXPEUXNQ MnPlS2k6OD1zNzFOwG0> NIrZ[W09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
Bel7404 M324c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnT4O|IhcA>? MXzEUXNQ M2\qcmdKQTB;MUig{txO NHPDWJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
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MGC803 NH3VO3NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYC3NkBp MkHxSG1UVw>? M3nGb2dKQTB;MUCuPEDPxE1? NXnXc2tSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlE{PzZpPkKzNFYyOzd4PD;hQi=>
SGC7901 NFfKV3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1u0e|czKGh? NV;ReG9kTE2VTx?= MYrHTVkxRTlwNDFOwG0> NGL6dW09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
SKHEP1 NInLVHVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;qO|IhcA>? MmHUSG1UVw>? NWrkRXh3T0l7ME2xO{DPxE1? NWTkNVU4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlE{PzZpPkKzNFYyOzd4PD;hQi=>
SMMC7721 NGTZ[WVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjU[3V{PzJiaB?= M3zVSmROW09? MoraS2k6OD1{MDFOwG0> NHLrems9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
NUGC3 Ml7ZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYKxNEDPxE1? MVfHTV0zNjl2IN88US=> M1zkXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MU[zN|MzLz5{M{G2N|M{OjxxYU6=
A2780 CisR MkfYT4lv[XOnIFHzd4F6 MlTSNVghcA>? MVnJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNESg{txO NYnKOlFuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
CAL27 sens MVTLbY5ie2ViQYPzZZk> MnyzNVghcA>? MmGwTY5pcWKrdHnvckBw\iCKRFHDJJdqfGhiSVO1NEBw\iByLkO2JO69VQ>? MkP4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
CAL27 CisR M3vDTmtqdmG|ZTDBd5NigQ>? M2XQ[lE5KGh? M4LRXWlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD62NUDPxE1? MlHaQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
KYSE-510 CisR MUPLbY5ie2ViQYPzZZk> NIfSXYkyQCCq M2XtcGlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD61PUDPxE1? NVfDfnRTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
KYSE-510 sens NG[xOVdMcW6jc3WgRZN{[Xl? NUTPcZdsOThiaB?= MmTITY5pcWKrdHnvckBw\iCKRFHDJJdqfGhiSVO1NEBw\iByLkeg{txO M2rNT|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
MDA-MB-231 sens MnvlT4lv[XOnIFHzd4F6 Mk\oNVghcA>? NF35XphKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuOlEh|ryP NFzxSGU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
MDA-MB-231 CisR NEPWSItMcW6jc3WgRZN{[Xl? NV\QcpNjOThiaB?= MlqyTY5pcWKrdHnvckBw\iCKRFHDJJdqfGhiSVO1NEBw\iByLk[xJO69VQ>? MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
ES2 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFH3cZc1QCCq MV;EUXNQ M{nIVmlEPTB;MUKuO{DPxE1? MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7M{S0PUc,OjN2OUO0OFk9N2F-
MDA-MB-231 M4PPRmtqdmG|ZTDBd5NigQ>? NYO2VoxkOTBizszN NFPoRYY{OCCvaX6= MXXEUXNQ MYfJcohq[mm2aX;uJI9nKEiGQVOyZS=> MmP6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUO0OFkoRjJ|NEmzOFQ6RC:jPh?=
MDA-MB-231 NGnwZ3hMcW6jc3WgRZN{[Xl? NGXJcpEyKM7:TR?= NX3ke2tZOjRiaB?= NXrOflZqTE2VTx?= MmHGTY5pcWKrdHnvckBw\iCKRFHDOkBie3Onc4Pl[EBieyCjY3X0fYxifGmxbjDs[ZZmdHNib3[geJVjfWyrbh?= NFTwO449[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5N|Q1QSd-MkO0PVM1PDl:L3G+
HS68 MmC4R5l1d3SxeHnjJGF{e2G7 MljzNVAh|ryP NXzCTIR6PzJiaB?= MkfKSG1UVw>? M1XWfWlEPTB;ND62JO69VQ>? NUmwXXJDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1O|A2PDJpPkKzOVcxPTR{PD;hQi=>
MEF NV65VmVtS3m2b4TvfIlkKEG|c3H5 MYmxNEDPxE1? MV63NkBp NHL1TZpFVVOR NVnGfHBxUUN3ME20MlQh|ryP NH7WeY89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{W3NFU1Oid-MkO1O|A2PDJ:L3G+
BGC823 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\EUXNQ MVvHTVUxRTNwMEmg{txO NIS4VVU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[wNVcxPid-MkO2NFE4ODZ:L3G+
High5 NFjye4dMcW6jc3WgRZN{[Xl? NHHSfGUzPCCq M4XhcmlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiSFTBR|Yhf2m2aDDJR|UxKG:oIECuNFkh|ryP MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZyMkWyN{c,OjN4MEK1NlM9N2F-
High5 NFjKUm9McW6jc3WgRZN{[Xl? MYKyOEBp NX:xOItrUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEOSC5aYToJGlEPTBib3[gNE4yOiEQvF2= NETPXnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[wNlUzOyd-MkO2NFI2OjN:L3G+
High5 NIG1dlFMcW6jc3WgRZN{[Xl? M33t[FI1KGh? MWXJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVOzJJdqfGhiSVO1NEBw\iByLkG3JO69VQ>? M3HFSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkCyOVI{Lz5{M{[wNlUzOzxxYU6=
A549 NVfBbXF4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2D3U2ROW09? NX;xfolqUUN3ME2xMlA4QSEQvF2= M4L1VlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkS0NlExLz5{M{[0OFIyODxxYU6=
HCT116 MmT1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHy2cGZFVVOR MYHJR|UxRTBwN{W3JO69VQ>? M4XXUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkS0NlExLz5{M{[0OFIyODxxYU6=
K562 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3[yNWROW09? MmryTWM2OD1yLk[0OUDPxE1? NYfrO2ZuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2OFQzOTBpPkKzOlQ1OjFyPD;hQi=>
GBC-SD NY\2O4w2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLxS2E1PSEQvF2= MV[3NkBp MVHEUXNQ MYPJR|UxRTF2Lkig{txO MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyN{K2NEc,OjN5MEeyOlA9N2F-
HGC27 NWXZVXZCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NELSbYs2KM7:TR?= M1HTV|czKGh? M1vkW2ROW09? MV7JR|UxRTVwNDFOwG0> NIntfXk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ewO|I3OCd-MkO3NFczPjB:L3G+
HL60 NFi4RYNMcW6jc3WgRZN{[Xl? MojSOUDPxE1? NHrVSXgzPCCq M2jZRWROW09? MoTETY5pcWKrdHnvckBw\iCKRFHD NHfxfG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ewO|I3OCd-MkO3NFczPjB:L3G+
Vero NWHUW3I3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NELXeIs4OiCq NWO3U5dXTE2VTx?= M{G1RmlEPTB;MD65JO69VQ>? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzd6NkS1Nkc,OjN5OE[0OVI9N2F-
NCI60 NFrIcoFEgXSxdH;4bYMhSXO|YYm= NYS4b45{PDhiaB?= MWrEUXNQ M2r0dGdKRTBwNUOg{txO MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDBzNUOyO{c,OjRyMUWzNlc9N2F-
SW620 NYDmPWJyS3m2b4TvfIlkKEG|c3H5 M13aNVQ5KGh? NHHXNYxFVVOR MXHHTV0xNjV2IN88US=> NWHtZ4ZFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNVU{OjdpPkK0NFE2OzJ5PD;hQi=>
U251 MWTDfZRwfG:6aXOgRZN{[Xl? NUHCSHRrPDhiaB?= M1fpc2ROW09? MWjHTV0yNjV|IN88US=> MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDBzNUOyO{c,OjRyMUWzNlc9N2F-
KM12 NF6zXnBEgXSxdH;4bYMhSXO|YYm= MofFOFghcA>? NHPBUlhFVVOR NYrpbpJjT0l;MT64PEDPxE1? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDBzNUOyO{c,OjRyMUWzNlc9N2F-
Hs578T MX3DfZRwfG:6aXOgRZN{[Xl? NGDtfm81QCCq NG\GZ|JFVVOR MWLHTV01Njh|IN88US=> M2nYNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEG1N|I4Lz5{NECxOVMzPzxxYU6=
MDA-MB-231 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYG0PEBp MmrvTWM2OD1yLkG2JO69VQ>? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7NUCxOkc,OjRyOUWwNVY9N2F-
MCF7 MlLvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUK3elY6PDhiaB?= MXrHTV0xNjR3IN88US=> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFzOUW1OUc,OjRzMUm1OVU9N2F-
MDA-MB-435 M1zROWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoToOFghcA>? M1rnU2dKRTBwOEig{txO MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFzOUW1OUc,OjRzMUm1OVU9N2F-
AsPC1 MmXoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV3nNFR4OzBizszN MX60PEBp M{PuT2ROW09? NX\t[VUzUUN3ME2zMlY3KM7:TR?= Ml\oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzOEWzO|goRjJ2MUi1N|c5RC:jPh?=
Jurkat E6.1 NHu0SlVMcW6jc3WgRZN{[Xl? Mn3XNkBp M{fE[GROW09? NUj3eWl6UW6qaXLpeIlwdiCxZjDISGFEPCC5aYToJGlEPTBib3[gNE4xPCEQvF2= NF3SSmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK2NVg3Oid-MkSyOlE5PjJ:L3G+
KG1 NGLNb49Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1;mSVQ5KGh? NWjHdHd7UUN3ME2xMlE2KM7:TR?= NUHSNIliRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS1NlUxODNpPkK0OVI2ODB|PD;hQi=>
HEL NEHpS2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXe0PEBp M4TMUWROW09? M1nyd2lEPTB;MD60PUDPxE1? NUTDNlJFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2PVQxPTVpPkK0Olk1ODV3PD;hQi=>
HSC3 NHvnWG9EgXSxdH;4bYMhSXO|YYm= M1TIdFEzKGh? M4nORmROW09? NIHa[pBKSzVyPUCuO|Eh|ryP NH22Vmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2OlU3OCd-MkS3OlY2PjB:L3G+
HT-29 NU\1S3hFS3m2b4TvfIlkKEG|c3H5 NE\qNpUyOiCq NV;CPJhXTE2VTx?= NVjYXHUzUUN3ME2wMlczKM7:TR?= M1v2NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{[2OVYxLz5{NEe2OlU3ODxxYU6=
KB NWjRWIJGS3m2b4TvfIlkKEG|c3H5 M3foXlEzKGh? M4TOPGROW09? M1fWdWlEPTB;MD63OUDPxE1? Mn\aQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5Nk[1OlAoRjJ2N{[2OVYxRC:jPh?=
H460 MWrDfZRwfG:6aXOgRZN{[Xl? NGPzdHkyOiCq M4DZNWROW09? M{nDUmlEPTB;MD64NkDPxE1? NWrXVYlERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OlY2PjBpPkK0O|Y3PTZyPD;hQi=>
MONE1 MXnDfZRwfG:6aXOgRZN{[Xl? MUWxNkBp M4W5N2ROW09? M1rBOmlEPTB;MT6xPEDPxE1? NWDPTnY{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OlY2PjBpPkK0O|Y3PTZyPD;hQi=>
MKN45 NVrmVXZuS3m2b4TvfIlkKEG|c3H5 Ml34NVIhcA>? Mmf5SG1UVw>? MlizTWM2OD1zLkK1JO69VQ>? NX[2XZlkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OlY2PjBpPkK0O|Y3PTZyPD;hQi=>
TSGH NIPBOGlEgXSxdH;4bYMhSXO|YYm= NXjhSHBxOTJiaB?= MVLEUXNQ MnTDTWM2OD1{LkC5JO69VQ>? NY\aR4F3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OlY2PjBpPkK0O|Y3PTZyPD;hQi=>
HL60 NGHofYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHzUc3Y4OiCq M4HnTGROW09? MU\HTVUxRTBwOUOg{txO NIHwb3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFUxOCd-MkS5NFA2ODB:L3G+
HEK293 NGPLSplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDsO|IhcA>? MknlSG1UVw>? MVXHTVUxRTF|IN88US=> MkHpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC1NFAoRjJ2OUCwOVAxRC:jPh?=
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NCI-H2347 MnvWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUPkbnN3UUN3ME2yN{4zPjd2IN88US=> Mkj4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
U-118-MG NXXTTWl5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3faPGlEPTB;MkOuO|IyPyEQvF2= MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
NCI-H596 NYTSfFBFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWDE[Ww{UUN3ME2yN{46ODV3IN88US=> NYrzPHpuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
MZ1-PC NWnVc2NTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{j5[2lEPTB;MkSuOlc2QSEQvF2= NGf4[5k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
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NCI-H82 M1H5e2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVPJR|UxRTJ4LkSyPFgh|ryP NX7YR5JPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
COLO-678 NEDFZXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVnJR|UxRTJ5LkS5NVkh|ryP M2rPR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
KURAMOCHI NEPuN|NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWTJR|UxRTJ6LkGyOkDPxE1? MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
NCI-H1650 MnjZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{TPW2lEPTB;M{CuNVkzPCEQvF2= Mm\SQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
HCC1954 MkLsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3jF[2lEPTB;M{GuN|Y3PiEQvF2= NV3NZ|hKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
GAK MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17GVmlEPTB;M{KuNlYyPiEQvF2= MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
YAPC MonzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{XJXmlEPTB;M{OuO|Y1QCEQvF2= NFu0T409[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NCI-H2228 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXSWlh6UUN3ME2zOE4{PjB3IN88US=> NVrDZZJ[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
VMRC-RCZ NEHkV5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIHQPFlKSzVyPUO2MlMxOjJizszN NUHIfFlYRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
NMC-G1 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnz2TWM2OD1|Nz6wOVI6KM7:TR?= NVzydIpJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
D-336MG MlnxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjMTWM2OD12MT64OFQzKM7:TR?= M2PvNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
UACC-62 M2\yWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGS4[lBKSzVyPUSyMlU2ODVizszN MljKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
C-33-A NHLlT5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrHRlV[UUN3ME20OU4{OjV4IN88US=> MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
HCC1937 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFzFWpZKSzVyPUS3MlE6PThizszN M{T2UFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
HDLM-2 NVrBVZNNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jpPGlEPTB;NEeuNlI5PyEQvF2= M33PZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H727 MkXwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MojETWM2OD12Nz6yOFUyKM7:TR?= MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
NY NWW2WlY3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnP0TWM2OD12OT6zPVA5KM7:TR?= M2m3RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H2291 MkDWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{L1bGlEPTB;NEmuPFMxOSEQvF2= M3i1RlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
Assay
Methods Test Index PMID
Western blot Ac-Histone H4 / Ac-Histone H3 ; phospho-CDK1 ; phospho-Cdc25c / Cdc25c ; Cyclin B1 ; c-Myc / c-Raf / Akt / Cyclin D1 / CDK4 ; p-eIF2α / ATF4 / CHOP ; p21 21598070 19440035 20072656
Immunofluorescence HDAC1 / CK19 ; Nrf2 ; α-SMA ; Ac-STAT3 / IGF2 29917299 20566897 26330748 27086926
Growth inhibition assay Cell apoptosis ; Cell viability 19440035 27086926
ELISA IGF2 ; IL-13 / IL-10 / IL-5 ; M-CSF ; MMP-9 27086926 18541724 23042302
In vivo

Administration of Vorinostat (~100 mg/kg/day) significantly inhibits the growth of CWR22 human prostate xenografts in nude mice with tumor reductions of 78%, 97% and 97%, at doses of 25 mg/kg/day, 50 mg/kg/day and 100 mg/kg/day, respectively, compared with control. Vorinostat induces the accumulation of acetylated core histones and prostate-specific antigen mRNA expression in CWR22 cells, resulting in higher levels of serum prostate-specific antigen than predicted from tumor volume alone. [2] Oral administration of Vorinostat (0.67g/L) crosses the blood-brain barrier, increases histone acetylation in the brain, and dramatically improves the motor impairment in the R6/2 mice model of Huntington's disease. [5]

Protocol (from reference)

Kinase Assay:

[1]

  • Immunoprecipitation-HDAC assays:

    The lysate of Jurkat cells is incubated for 1 hour on ice and cleared by centrifugation at 12,000 g for 10 minutes at 4 °C. Supernatants are precleared with 30 μL of 50% protein G-Sepharose slurry for 1 hour at 4 °C. Beads are pelleted by centrifugation and supernatants are incubated for 1 hour at 4 °C with 10 μg of IgG fraction from anti-HDAC1 or HDAC3 polyclonal antisera (preincubated 2 hours at room temperature with either the homologous or heterologous immunizing peptide). Both antisera are raised in rabbits against the carboxylterminal peptide of HDAC1 and HDAC3 by using synthetic peptides coupled to keyhole limpet hemocyanin. 30 μL of 50% protein G-Sepharose slurry is added for 1 hour at 4 °C. Immune complexes are pelleted by centrifugation and washed three times with 1 mL of lysis buffer. Beads are resuspended in 200 μL of HDAC buffer (20 mM Tris-HCl, pH 8.0/150 mM NaCl/10% glycerol), and the HDAC assay is performed with an 3H-acetylated peptide corresponding to amino acids 1-24 of histone H4. Released [3H]acetic acid is quantified by scintillation counting. For inhibitions studies, the immunoprecipitated complexes are preincubated with the different concentrations of Vorinostat for 30 minutes at 4 °C.

Cell Research:

[2]

  • Cell lines: LNCaP, PC-3, and TSU-Pr1
  • Concentrations: Dissolved in DMSO, final concentrations ~7.5 μM
  • Incubation Time: 1, 2, 3 and 4 days
  • Method:

    Cells are exposed to various concentrations of Vorinostat for 1, 2, 3 and 4 days. Cell viability is assessed by trypan blue dye exclusion.

Animal Research:

[2]

  • Animal Models: Male BALB/c nude (nu/nu) mice implanted with CWR22 tumor cells
  • Dosages: 25, 50, and 100 mg/kg/day
  • Administration: Injection i.p.

Solubility (25°C)

In vitro

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

5mg/mL

Chemical Information

Molecular Weight 264.3
Formula

C14H20N2O3

CAS No. 149647-78-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC=C(C=C1)NC(=O)CCCCCCC(=O)NO

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03022565 Withdrawn Drug: Vorinostat Uveal Melanoma University of Miami|University of Miami Sylvester Comprehensive Cancer Center January 2020 Early Phase 1
NCT03167437 Recruiting Drug: Vorinostat|Drug: Ustekinumab Crohn''s Disease National Institute of Allergy and Infectious Diseases (NIAID)|National Institutes of Health Clinical Center (CC) October 30 2017 Phase 1|Phase 2
NCT03056495 Unknown status Drug: N-hydroxy-N''-phenyl-octanediamide (Vorinostat) Alzheimer Disease German Center for Neurodegenerative Diseases (DZNE)|University Hospital Bonn|University of Göttingen September 28 2017 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
What’s the recommendation about reconstitution of the compound for in vivo animal study?

Answer:
We recommend the following vehicle for S1047: 2% DMSO, 40% PEG 300, 5% Propylene glycol, 1% Tween 80 at 5 mg/mL.

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