Vorinostat (SAHA)

For research use only.

Catalog No.S1047 Synonyms: MK0683, Suberoylanilide hydroxamic acid, Zolinza

456 publications

Vorinostat (SAHA) Chemical Structure

CAS No. 149647-78-9

Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683, Zolinza) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.

Selleck's Vorinostat (SAHA) has been cited by 456 publications

Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683, Zolinza) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.
Targets
HDAC [1]
(Cell-free assay)
~10 nM
In vitro

Vorinostat inhibits the activities of HDAC1 and HDAC3 with IC50 of 10 nM and 20 nM, respectively. Vorinostat also results in a marked hyperacetylation of histone H4. [1] Vorinostat inhibits the growth of three prostate cancer cell lines LNCaP, PC-3 and TSU-Pr1 at micromolar concentrations (2.5-7.5 μM), and induces dose-dependent cell death in LNCaP cells. [2] Vorinostat treatment in MCF-7 cells inhibits cell proliferation at an IC50 of 0.75 μM resulting in the accumulation of cells in the G1 and G2-M phase of the cell cycle. Vorinostat also induces differentiation in the estrogen receptor-negative cell line SKBr-3 and the retinoblastoma-negative cell line MDA-468. [3] Vorinostat treatment at 1 μM for 8 hours or more is sufficient to irreversibly induce apoptosis of human multiple myeloma (MM) cells. The gene expression profiles of Vorinostat treated MM cells are not hallmarked by global transcriptional activation, but by coordinated transcriptional changes of specific functional groups of genes such as cytokine-induced proliferative/survival signaling cascades, oncogenes-tumor suppressor genes, regulators of apoptosis, DNA synthesis-repair and cell cycle, and proteasome-ubiquitin function. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H1299 MV7LbY5ie2ViQYPzZZk> MUCyNEBucW5? MY\EUXNQ M1fOemlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBJTEGFIHXufplu\SC5aYToJGlEPTBib3[gNE4yQTRizszN MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDV{MUSyNkc,OTR3MkG0NlI9N2F-
HT1080 M3r5Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1Hv[|czKGh? MUnEUXNQ NVPteZpyUUN3ME2yMlQh|ryP NITHdnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkK3NFE4PSd-MUKyO|AyPzV:L3G+
K562 MU\LbY5ie2ViQYPzZZk> MVS2JIg> NHzuUHVFVVOR MWXJcohq[mm2aX;uJI9nKGi3bXHuJIhqe3SxbnWg[IVi[2W2eXzhd4UhMG2reIT1doUhd2ZiSFTBR|Eh[W6mIFjERWMzMSC5aYToJGlEPTBib3[gNE4xOSEQvF2= NHu0fGc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkSxPVM5OCd-MUK0NVk{QDB:L3G+
A20 MYHLbY5ie2ViQYPzZZk> M2LUeVEhcA>? MmDOSG1UVw>? MnTYZY51cS2KRFHDNUBi[3Srdnn0fUB4cXSqIFnDOVAhd2ZiMD6xNVIh|ryP NIr3VpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUm2NFQ5QSd-MUG5OlA1QDl:L3G+
Friend leukemic cell NWrUZ3N{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUe1NEDPxE1? M2DRNVczKGh? MoHzSG1UVw>? MYfJR|UxRTBwOUmg{txO MnTJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJzMEm5NVMoRjF{MUC5PVE{RC:jPh?=
H1299 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2LJ[|czKGh? MkHYSG1UVw>? NFXqTW1KSzVyPUeuNlQh|ryP MoTGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTR3MkG0NlIoRjF2NUKxOFIzRC:jPh?=
HeLa NUTBXXo6U2mwYYPlJGF{e2G7 NVHZdnFTOzBibXnu NET2enFKdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6|dDDobZN1d26nIHTlZYNmfHmuYYPlJJdqfGhiSVO1NEBw\iByLkK4JO69VQ>? MnK5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV4MEO5OFkoRjF3NkCzPVQ6RC:jPh?=
LOX-IMVI MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVW0PEBp M1;oR2lEPTB;MT6zJO69VQ>? NGPOZ5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUexOVQ4OCd-MUW3NVU1PzB:L3G+
NCI-H226 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVi0PEBp MXzJR|UxRTJwNjFOwG0> MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTdzNUS3NEc,OTV5MUW0O|A9N2F-
RXF-631L MnvkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3O1RVQ5KGh? MnTNTWM2OD1{IN88US=> M2O5ZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{G1OFcxLz5zNUexOVQ4ODxxYU6=
SNB-78 M1[1TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3:5SlQ5KGh? NIexd4xKSzVyPUG2JO69VQ>? NILRVIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUexOVQ4OCd-MUW3NVU1PzB:L3G+
St-4 NWXxVY5rT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17xb|Q5KGh? NHHWUIRKSzVyPUWuNkDPxE1? M1XpUFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{G1OFcxLz5zNUexOVQ4ODxxYU6=
ARP1 NX;0e3BsTnWwY4Tpc44hSXO|YYm= NVK3U3dXOiEQvF2= MWW2JIg> NVzNSnFoUW6mdXP0bY9vKG:oIGCyNXdCTjFiZ3Xu[UBmgHC{ZYPzbY9v MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDd|NEiwOkc,OTR5M{S4NFY9N2F-
CEM NVnWbo1CU2mwYYPlJGF{e2G7 NFHxS2syKGh? NVTFfmQ5TE2VTx?= MYrJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwM{Og{txO NX\Ub|V2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NlAxPjRpPkG2OFIxODZ2PD;hQi=>
HCT15 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3vHflQ5KGh? MXrHTVUxRTBwOEKg{txO NXvFc29jRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[3NlMzOjdpPkG2O|I{OjJ5PD;hQi=>
NCI-H23 M1XQPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV[4XZE5PDhiaB?= NVfLU2F1T0l3ME2wMlkzKM7:TR?= M3:we|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4N{KzNlI4Lz5zNkeyN|IzPzxxYU6=
GM15850 MknwT4lv[XOnIFHzd4F6 MlyzNk42KM7:TR?= MYi5OkBp NV3MSmJ7TE2VTx?= NFfSPIZKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIN{BtgXOrbnWgNVQh[WOndInsZZRqd25ib3[gSnhPKGenbnW= MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl{MUO2O{c,OTZ7MkGzOlc9N2F-
GM15850 MWPLbY5ie2ViQYPzZZk> MUCyMlUh|ryP NFO5ZYE6PiCq MWTEUXNQ NH;CPFFKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIOEBtgXOrbnWgOUBi[2W2eXzheIlwdiCxZjDGXG4h\2WwZR?= NF\xUpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmyNVM3Pyd-MU[5NlE{Pjd:L3G+
GM15850 M4i5cWtqdmG|ZTDBd5NigQ>? MXuyMlUh|ryP MoT6PVYhcA>? MoW5SG1UVw>? MXnJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKNDDsfZNqdmViMUKgZYNmfHmuYYTpc44hd2ZiRmjOJIdmdmV? NHTnS3Y9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmyNVM3Pyd-MU[5NlE{Pjd:L3G+
GM15850 NVLaS|QxU2mwYYPlJGF{e2G7 M3raO|IvPSEQvF2= M{TWc|k3KGh? MluySG1UVw>? MYDJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKMzDsfZNqdmViOTDhZ4V1gWyjdHnvckBw\iCIWF6g[4Vv\Q>? NIGwSng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmyNVM3Pyd-MU[5NlE{Pjd:L3G+
GM15850 NUftUIJUU2mwYYPlJGF{e2G7 NHe1OHkzNjVizszN NWfhUIZKQTZiaB?= NIftV5RFVVOR MUXJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKNDDsfZNqdmViODDhZ4V1gWyjdHnvckBw\iCIWF6g[4Vv\Q>? NHvsc3Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmyNVM3Pyd-MU[5NlE{Pjd:L3G+
GM15850 MYLLbY5ie2ViQYPzZZk> MomwNk42KM7:TR?= MWq5OkBp NWPmTVVUTE2VTx?= NWj3[FJCUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFQhdHm|aX7lJFE3KGGlZYT5cIF1cW:wIH;mJGZZViCpZX7l NXTZXZhyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5NlE{PjdpPkG2PVIyOzZ5PD;hQi=>
U937 NYm1OmxHTnWwY4Tpc44hSXO|YYm= NXf0RWVwPSEQvF2= NHzHUlUzPCCq M{Dkb2ROW09? M1PuSGNmdGxiY4njcIUh[XK{ZYP0JIJ6KGGlY4XteYxifGmxbjDheEBUN0d{L12gdIhie2V? NVjQW4JqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUewNFQ4OThpPkG3NFA1PzF6PD;hQi=>
H661 Ml3tS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWq0PEBp M4Dsc2lEPTB;MTFOwG0> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzF3N{CwPUc,OTdzNUewNFk9N2F-
U937 NVHCW5NkTnWwY4Tpc44hSXO|YYm= M2P1fVUh|ryP MYKyOEBp NEnu[W9FVVOR MnnwTY5kemWjc3WgbY4hcGm|dH;u[UBJOyCjY3X0fYxifGmxbh?= MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzR2N{e1NEc,OTd2NEe3OVA9N2F-
U937 Ml;FSpVv[3Srb36gRZN{[Xl? MnTJNUDPxE1? MoLONlQhcA>? M3vH[WROW09? MU\F[oZm[3Rib36gZYxxcGFidIXieYxqdiCjY3X0fYxifGmxbh?= NWLLXVRkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OFc4PTBpPkG3OFQ4PzVyPD;hQi=>
RAW264.7 NEDMPHRHfW6ldHnvckBCe3OjeR?= NFvaN|cyOCEQvF2= NHPvblYzPGh? M3XYdZdifGW{ MlHHTY5pcWKrdHnvckBw\iCOUGOtd5RqdXWuYYTl[EBVVk[jbIDoZUBxem:mdXP0bY9vKHerdHigTWM2OCCxZjCwMlc3KM7:TR?= M17UPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEe3OVE5Lz5zN{S3O|UyQDxxYU6=
RAW264.7 NHS5b5BHfW6ldHnvckBCe3OjeR?= MWqxNEDPxE1? NYPafGJlOjSq M2XXVJdifGW{ NHvVNndKdmirYnn0bY9vKG:oIFzQV{1{fGmvdXzheIVlKE6RIIDyc4R2[3Srb36ge4l1cCCLQ{WwJI9nKDFwOEig{txO MnK0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2N{e1NVgoRjF5NEe3OVE5RC:jPh?=
SC9 NFTCXo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfEZphOPDhiaB?= NHf0XYtKSzVyPUCuOlA3KM7:TR?= Mo\DQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3MEeyNVkoRjF5NUC3NlE6RC:jPh?=
ST14A Moe1SpVv[3Srb36gRZN{[Xl? NGjy[FU2KG6P NIPLeYc4OiCq NWTPU29TUW6lcnXhd4UhcW5iUlXTWE9PWlOIIH3SUmEh\XiycnXzd4lwdg>? MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV4NUm5N{c,OTd3NkW5PVM9N2F-
ST14A NXLXT4xmTnWwY4Tpc44hSXO|YYm= NEfhSXQ2KG6P NULtUXQ6PzJiaB?= NUGxTG4xUW6lcnXhd4UhcW5iRULGNUBuWk6DIHX4dJJme3Orb36= NFzQOoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W2OVk6Oyd-MUe1OlU6QTN:L3G+
PANC1 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXKxJO69VQ>? MV\JR|UxRTBwNEWg{txO MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV6OEe0OEc,OTd3OEi3OFQ9N2F-
PANC1 Mn21SpVv[3Srb36gRZN{[Xl? Mn7TNUDPxE1? MViyOEBp MYDJcoNz\WG|ZTDpckBxOjGZQV[xM2NKWDFiZ3Xu[UBmgHC{ZYPzbY9v MlzzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OEi3OFQoRjF5NUi4O|Q1RC:jPh?=
PANC1 MUHGeY5kfGmxbjDBd5NigQ>? NEfWdHUyKM7:TR?= MXyyOEBp Mm\ZTY5kemWjc3WgbY4heDJ5a3nwNUBo\W6nIHX4dJJme3Orb36= NGHxUYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W4PFc1PCd-MUe1PFg4PDR:L3G+
PT45 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkTuTWM2OD12IN88US=> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV6OEe0OEc,OTd3OEi3OFQ9N2F-
ACHN Mn34S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWKyNUBp M1ToN2dKPTB;MD62OUDPxE1? M2\CbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5Nk[yOlA3Lz5zN{[2NlYxPjxxYU6=
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Cal27 NI\XOHZEgXSxdH;4bYMhSXO|YYm= NHf6Z|Y4OiCq NETLPFBKSzVyPUOuNkDPxE1? NF3mRmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[5NVc3Oyd-MUe2PVE4PjN:L3G+
CCRF-CEM MYDDfZRwfG:6aXOgRZN{[Xl? M{GwfVczKGh? NXn2cWZ1UUN3ME2wMlgh|ryP MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ7MUe2N{c,OTd4OUG3OlM9N2F-
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Hec-1-A NH;tUnVEgXSxdH;4bYMhSXO|YYm= NGfTdWY4OiCq MUDJR|UxRTFizszN NXPLNJVPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2PVE4PjNpPkG3OlkyPzZ|PD;hQi=>
HeLa Mm\CT4lv[XOnIFHzd4F6 MVOxNEDPxE1? MmDyN|AhdWmw MYHEUXNQ NH\xSIlKdmirYnn0bY9vKG:oIILlZ49u[mmwYX70JGhFSUNzIIfpeIghUUN3MDDv[kAxNjB{IN88US=> MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ7MUe2N{c,OTd4OUG3OlM9N2F-
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Primary bronchial epithelial cell M{HsUGtqdmG|ZTDBd5NigQ>? MmDBOUDPxE1? M{i1W|I1KGh? M{fqN2ROW09? MWrJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6mdXP0bY9vKG:oIH31eIFvfCCIZHXseIE2ODhiQ1\UVkBxem:2ZXnuJIFxcWOjbDDzeZJn[WOnIHzvZ4FtcXqjdHnvci=> M{PzdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OU[2O|g6Lz5zOUm2Olc5QTxxYU6=
Primary bronchial epithelial cell Mn20T4lv[XOnIFHzd4F6 NFj5PGEyKM7:TR?= MYS5OkBp M3jMTWROW09? MX3Jcohq[mm2aX;uJI9nKEiGQVO3JIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIIPoc5J1KGOrcnP1bZQh[3W{cnXueJM> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl4Nke4PUc,OTl7Nk[3PFk9N2F-
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LNCAP MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIi5NlA4OiCq Mmi2TWM2OD1yLkW4JO69VQ>? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{e3PEc,OjBzNEO3O|g9N2F-
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NCI-H358 MmXHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\tSZhqPzJiaB?= MYrJR|UxRTJwNTFOwG0> MlrXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO3O|goRjJyMUSzO|c5RC:jPh?=
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Caco-2 NXTpN3hyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mke5NVAxKM7:TR?= NVT5bYFkPDiq NW\sfXZyTE2VTx?= NVW5O|NRUUN3ME24MlEh|ryP Mnj4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
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Caco-2 MoTCT4lv[XOnIFHzd4F6 M3XGSFAvOzF4IH7NJJRwKDNzLk[g{txO NH\DSog1KGh? MWnEUXNQ Mom3SZghfmm4bzDpcohq[mm2aX;uJI9nKGi3bXHuJGhFSUN|IIfpeIghUUN3MDDv[kAxNjF2NzFOwG0> MkDEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
Caco-2 MXfLbY5ie2ViQYPzZZk> MYG2MlMzKG6PIITvJFY{OiEQvF2= NXHZbXc2PCCq MV7EUXNQ NUjJW2xzTXhidnn2c{BqdmirYnn0bY9vKG:oIHj1cYFvKEiGQVO1JJdqfGhiSVO1NEBw\iBzMj62JO69VQ>? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
Caco-2 MYXLbY5ie2ViQYPzZZk> MXq2MlMzKG6PIITvJFY{OiEQvF2= MkTNOEBp MUDEUXNQ MWLFfEB3cX[xIHnubIljcXSrb36gc4YhcHWvYX6gTGRCSzdid3n0bEBKSzVyIH;mJFQyKM7:TR?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
Caco-2 MoPvT4lv[XOnIFHzd4F6 M1viTFAvOzF4IH7NJJRwKDNzLk[g{txO NI\RUoM1KGh? NG\sOJlFVVOR NVnzSoR1TXhidnn2c{BqdmirYnn0bY9vKG:oIHj1cYFvKEiGQVO2 M3u1ZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzPFQxLz5{MEG0N|g1ODxxYU6=
HepG2 M1zDbGtqdmG|ZTDBd5NigQ>? NEHSbmk2KM7:TR?= NUDQU5c6Pmh? MVPEUXNQ MYnJcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDobZN1d26nIHHj[ZR6dGG2aX;u M4fSfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzPFQxLz5{MEG0N|g1ODxxYU6=
HepG2 NHPIS3pMcW6jc3WgRZN{[Xl? MmnpOUDPxE1? MVW2bC=> NGTL[JRFVVOR M4\HPWlv\HWldHnvckBw\iCKRFHDMY1m\GmjdHXkJIhqe3SxbnWgbJlx\XKjY3X0fYxifGmxbh?= NXXtbIFQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
HepG2 NX7kNWhDU2mwYYPlJGF{e2G7 NHKxcGE2KM7:TR?= MWW2bC=> NXLTU2RlTE2VTx?= MXrJcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDhcJBp[S22dXL1cIlvKGGlZYT5cIF1cW:w NHrLNpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N|g1OCd-MkCxOFM5PDB:L3G+
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COLO205 NGHGZW1McW6jc3WgRZN{[Xl? NHW3eVIyOCEQvF2= NVe4O4s5OjRiaB?= M{W4bGROW09? MmnzTY5pcWKrdHnvckBw\iCKRFHDNUBie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gbIl{fG:wZTDIN{Bi[2W2eXzheIlwdiC5aYToJGlEPTBib3[gNk4zKM7:TR?= NVm1XmJ[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0OVE{PzhpPkKwOFUyOzd6PD;hQi=>
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A549 NXm1SGRZTnWwY4Tpc44hSXO|YYm= MWK1JO69VQ>? M3TR[lMhcA>? Mn[zSG1UVw>? NGToUGFKdmS3Y4Tpc44hd2ZiYYX0c5Bp[We7IHnuJIh2dWGwIFG1OFkh[2WubIOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iTFOzTWk> MnTKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6NUWyNFgoRjJyOEW1NlA5RC:jPh?=
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U937 NV3CWXFoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkfQO|IhcA>? M{XwTWROW09? M2\uVWdKPTB;MD6zPUDPxE1? MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh6NEKwPEc,OjB6OESyNFg9N2F-
A431 MnHOR5l1d3SxeHnjJGF{e2G7 MYm3NkBp NXn3bFZlS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTR|MTDj[YxteyCxdnXy[ZhxemW|c3nu[{BGT0[UIIfpeIghUUN3MDDv[kA1NjJizszN NFXsRmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFYzQSd-MkGwPFA3Ojl:L3G+
A549 M2LEcWN6fG:2b4jpZ{BCe3OjeR?= NGHHd5Y4OiCq MkX2R5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{BwfmW{ZYjwdoV{e2mwZzDISGFEKHerdHigTWM2OCCxZjCxMlgh|ryP MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
CAL27 MVTDfZRwfG:6aXOgRZN{[Xl? NW[0S|dtPzJiaB?= NG\WdGZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBESUx{NzDj[YxteyCxdnXy[ZhxemW|c3nu[{BGT0[UIIfpeIghUUN3MDDv[kA{NjJizszN NH\rRpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFYzQSd-MkGwPFA3Ojl:L3G+
HeLa M4nh[2N6fG:2b4jpZ{BCe3OjeR?= NWLlfo5KPzJiaB?= MnLFR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{BwfmW{ZYjwdoV{e2mwZzDISGFEKHerdHigTWM2OCCxZjCxMlUh|ryP M1zoOFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEiwOlI6Lz5{MUC4NFYzQTxxYU6=
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COR-L23 NIX4WJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfONVAh|ryP NFz2bWI4OiCq MWXEUXNQ NFPCZWlIUTVyPUGuNUDPxE1? NFixTIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFY1Pyd-MkGwPFA3PDd:L3G+
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HCT116 NXzQe5d[U2mwYYPlJGF{e2G7 Mmf6N|Ah|ryP M{CzclMxKGh? M{fYc2ROW09? MkXZTY5pcWKrdHnvckBw\iCKRFHDNUBie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gbIl{fG:wZTDIN2s6KGGlZYT5cIF1cW:w NHL4S2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFY1Pyd-MkGwPFA3PDd:L3G+
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U937 MlvNT4lv[XOnIFHzd4F6 M1HJelUh|ryP M4rlRVI1KGh? MkS4SG1UVw>? MV3Jcohq[mm2aX;uJI9nKGirc4TvcoUh[WOndInseJJidnOoZYLhd4Uh[XO|ZYPz[YQh[XNiaX7jdoVie2Vib3[gZYNmfHmuYYTpc44hd2ZiaHnzeI9v\SCKMzDheEBMOTh? MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7MkS5Nkc,OjF{OUK0PVI9N2F-
U937 NF3remRMcW6jc3WgRZN{[Xl? NV\VNJpGPSEQvF2= M4XWPFI1KGh? MlTiSG1UVw>? MVjJcohq[mm2aX;uJI9nKGirc4TvcoUh[WOndInseJJidnOoZYLhd4Uh[XO|ZYPz[YQh[XNiaX7jdoVie2Vib3[gdIFvNWGlZYT5cIF1\WRiaHnzeI9v\SCKNDDs[ZZmdA>? NYDZToRDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPVI1QTJpPkKxNlkzPDl{PD;hQi=>
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A2780 MkLGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2LUbVk3KGh? MUHEUXNQ MYXJR|UxRTFwNkKg{txO MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTZ|NESzNEc,OjF4M{S0N|A9N2F-
COLO205 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYG5OkBp NXnOeFJvTE2VTx?= MXrJR|UxRTJwMUKg{txO MnrjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF4M{S0N|AoRjJzNkO0OFMxRC:jPh?=
Hep3B M1i4fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1W5NlQ5KGh? NYrFO4Q2TE2VTx?= MkfxS2k2OD1zLkKxJO69VQ>? MkOzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF5MUKxOFYoRjJzN{GyNVQ3RC:jPh?=
GM11272 NHTse41HfW6ldHnvckBCe3OjeR?= NInNSHoyODBibl2= M3nuUlczKGh? Mnj3SG1UVw>? NFvVb|JT\WGldHn2ZZRqd25ib3[gUYVEeDJibYX0ZY51KGW6cILld5Nqd25iYYSgNVAhfG9iMUCwJI5O Ml7IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF6NEC3NVYoRjJzOESwO|E3RC:jPh?=
CFBE41o NU\QcmpSU2mwYYPlJGF{e2G7 M1:1XVUh|ryP M3fWfWROW09? NIGydYpFd3ewIILl[5Vt[XSrb36gc4YhUESDQ{egZZQhOC5{IITvJFUhfU1? NVz0SWM1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5PFQ6PThpPkKxPVg1QTV6PD;hQi=>
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DU145 M4ry[2tqdmG|ZTDBd5NigQ>? MU[yMlUh|ryP Mmi3NlQhcA>? MmPaSG1UVw>? NHXGPZJKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNidYDy[Yd2dGG2aX;uJI9nKHB{MYfh[lEheHKxdHXpckBmgHC{ZYPzbY9v MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ4MEG2Okc,OjJ{NkCxOlY9N2F-
DU145 M3;zRmtqdmG|ZTDBd5NigQ>? NUjTfWtTPSEQvF2= M{DyXlI1KGh? MnnvSG1UVw>? NYjlSYdzUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHjpd5RwdmViSESgZYNmfHmuYYTpc44> MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ4MEG2Okc,OjJ{NkCxOlY9N2F-
DU145 MY\LbY5ie2ViQYPzZZk> NGrMN3c2KM7:TR?= MYOyOEBp NV7HfZVWTE2VTx?= Mof5TY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGirc4TvcoUhUDNiaInw[ZJi[2W2eXzheIlwdg>? M3fWXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[wNVY3Lz5{MkK2NFE3PjxxYU6=
786-0 MlXWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml\0Ok4zKM7:TR?= Mlu0O|IhcA>? M17WdWROW09? MlvRS2k2OD1|LkKg{txO NX3xNXhqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
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A549/ATCC MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M13oblYvOiEQvF2= MnTtO|IhcA>? NXL2SVR6TE2VTx?= MXXHTVUxRTFwNjFOwG0> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
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SGC7901 NFywTW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWe3NkBp MUDEUXNQ NIjLWJlIUTlyPUmuOEDPxE1? NIThPFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
SKHEP1 NVnydFcxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU[3NkBp NV\XWWF6TE2VTx?= NELtXHJIUTlyPUG3JO69VQ>? M{i2NlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|ME[xN|c3Lz5{M{C2NVM4PjxxYU6=
SMMC7721 M3Hiemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWX2T3RPPzJiaB?= M{n2Z2ROW09? NI[0SpBIUTlyPUKwJO69VQ>? NU\3NnhSRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlE{PzZpPkKzNFYyOzd4PD;hQi=>
NUGC3 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVWxNEDPxE1? MnPSS2k:Oi57NDFOwG0> NXHiZYV4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlM{OzJpPkKzNVY{OzN{PD;hQi=>
A2780 CisR MlnnT4lv[XOnIFHzd4F6 NYGyWmZsOThiaB?= MYnJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNESg{txO MkT2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
CAL27 sens NYq4ZWI6U2mwYYPlJGF{e2G7 NGO5UG8yQCCq NVzVVXhPUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlM3KM7:TR?= NXThT4pwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
CAL27 CisR Mn7wT4lv[XOnIFHzd4F6 MljRNVghcA>? MknLTY5pcWKrdHnvckBw\iCKRFHDJJdqfGhiSVO1NEBw\iByLk[xJO69VQ>? NFfIVIM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
KYSE-510 CisR NHPWWZBMcW6jc3WgRZN{[Xl? M3H1c|E5KGh? NXnX[lBGUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlU6KM7:TR?= M2P4elxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
KYSE-510 sens NHrFUGNMcW6jc3WgRZN{[Xl? NFT6bHYyQCCq NYrCdZg5UW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlch|ryP MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
MDA-MB-231 sens MVrLbY5ie2ViQYPzZZk> M{DubFE5KGh? MkjZTY5pcWKrdHnvckBw\iCKRFHDJJdqfGhiSVO1NEBw\iByLk[xJO69VQ>? M{\QSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
MDA-MB-231 CisR MY\LbY5ie2ViQYPzZZk> MVWxPEBp MnXtTY5pcWKrdHnvckBw\iCKRFHDJJdqfGhiSVO1NEBw\iByLk[xJO69VQ>? M4LHN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
ES2 MlTtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVi5b4tbPDhiaB?= Ml7ESG1UVw>? M4LDUmlEPTB;MUKuO{DPxE1? NIradVA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5N|Q1QSd-MkO0PVM1PDl:L3G+
MDA-MB-231 M2TJOGtqdmG|ZTDBd5NigQ>? NI\oO3EyOCEQvF2= M1Tpc|MxKG2rbh?= MnfnSG1UVw>? MmH5TY5pcWKrdHnvckBw\iCKRFHDNoE> MmK2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUO0OFkoRjJ|NEmzOFQ6RC:jPh?=
MDA-MB-231 NFfzO2FMcW6jc3WgRZN{[Xl? M37wcVEh|ryP NFfkPJczPCCq MXnEUXNQ NEe3SJpKdmirYnn0bY9vKG:oIFjERWM3KGG|c3Xzd4VlKGG|IHHj[ZR6dGG2aX;uJIxmfmWuczDv[kB1fWK3bHnu MnL0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUO0OFkoRjJ|NEmzOFQ6RC:jPh?=
HS68 NHPhOphEgXSxdH;4bYMhSXO|YYm= NF20VW8yOCEQvF2= M2XsSVczKGh? MnnCSG1UVw>? MorUTWM2OD12Lk[g{txO MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV5MEW0Nkc,OjN3N{C1OFI9N2F-
MEF NGX3ZWtEgXSxdH;4bYMhSXO|YYm= MVGxNEDPxE1? MUm3NkBp MYPEUXNQ MW\JR|UxRTRwNDFOwG0> MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV5MEW0Nkc,OjN3N{C1OFI9N2F-
BGC823 MoLzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXnEUXNQ NFPzNoZIUTVyPUOuNFkh|ryP MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZyMUewOkc,OjN4MEG3NFY9N2F-
High5 NW\rRo5xU2mwYYPlJGF{e2G7 M{jEN|I1KGh? NGjYVoZKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWM3KHerdHigTWM2OCCxZjCwMlA6KM7:TR?= M4joZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkCyOVI{Lz5{M{[wNlUzOzxxYU6=
High5 MXzLbY5ie2ViQYPzZZk> M3PBR|I1KGh? NFK2dmVKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWMyKHerdHigTWM2OCCxZjCwMlEzKM7:TR?= M1f5WFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkCyOVI{Lz5{M{[wNlUzOzxxYU6=
High5 NVL1WodkU2mwYYPlJGF{e2G7 NXvrU2E4OjRiaB?= NXHudndzUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEOyC5aYToJGlEPTBib3[gNE4yPyEQvF2= NF\1PVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[wNlUzOyd-MkO2NFI2OjN:L3G+
A549 Mm[xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M320NmROW09? MWXJR|UxRTFwMEe5JO69VQ>? M2[3[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkS0NlExLz5{M{[0OFIyODxxYU6=
HCT116 Mnr6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTrNmxxTE2VTx?= NWPSeXlHUUN3ME2wMlc2PyEQvF2= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZ2NEKxNEc,OjN4NESyNVA9N2F-
K562 NHvaSGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;EWVlNTE2VTx?= NID4XWpKSzVyPUCuOlQ2KM7:TR?= NEm5U5g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[0OFIyOCd-MkO2OFQzOTB:L3G+
GBC-SD MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF\vZYw2KM7:TR?= M3f4TVczKGh? M1z2O2ROW09? NELH[VZKSzVyPUG0Mlgh|ryP MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyN{K2NEc,OjN5MEeyOlA9N2F-
HGC27 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml21OUDPxE1? MWm3NkBp NYnSb2lOTE2VTx?= NH73OJZKSzVyPUWuOEDPxE1? M3ywXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C3NlYxLz5{M{ewO|I3ODxxYU6=
HL60 NGGz[YJMcW6jc3WgRZN{[Xl? NIPkTW82KM7:TR?= NFu5N3czPCCq M3\he2ROW09? M2n6dWlvcGmkaYTpc44hd2ZiSFTBRy=> MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyN{K2NEc,OjN5MEeyOlA9N2F-
Vero M3\CR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3K1blczKGh? MVjEUXNQ MVvJR|UxRTBwOTFOwG0> MmXDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OE[0OVIoRjJ|N{i2OFUzRC:jPh?=
NCI60 NVnTSpZqS3m2b4TvfIlkKEG|c3H5 NXLYSFBHPDhiaB?= M4KxRWROW09? MkjCS2k:OC53MzFOwG0> MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDBzNUOyO{c,OjRyMUWzNlc9N2F-
SW620 NYHjNIpWS3m2b4TvfIlkKEG|c3H5 MV20PEBp NIS1VVJFVVOR M1fWXGdKRTBwNUSg{txO NIDpNGM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECxOVMzPyd-MkSwNVU{Ojd:L3G+
U251 NICxXIJEgXSxdH;4bYMhSXO|YYm= NUe4OoxPPDhiaB?= NVv0ZYRiTE2VTx?= NULDOnNTT0l;MT61N{DPxE1? NUDDVHJ6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNVU{OjdpPkK0NFE2OzJ5PD;hQi=>
KM12 NVvSO4lZS3m2b4TvfIlkKEG|c3H5 MmXSOFghcA>? MkXtSG1UVw>? NX\MPIVTT0l;MT64PEDPxE1? NX;BSGpkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNVU{OjdpPkK0NFE2OzJ5PD;hQi=>
Hs578T Mm\CR5l1d3SxeHnjJGF{e2G7 MYW0PEBp NEPsWpdFVVOR M1OxTGdKRTRwOEOg{txO M4fSS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEG1N|I4Lz5{NECxOVMzPzxxYU6=
MDA-MB-231 NUDtW|l3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUO0PEBp MV3JR|UxRTBwMU[g{txO NXuyV5U5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwPVUxOTZpPkK0NFk2ODF4PD;hQi=>
MCF7 NVrFWGo4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\hVZdyPDhiaB?= MmqxS2k:OC52NTFOwG0> NXPQeGh{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNVk2PTVpPkK0NVE6PTV3PD;hQi=>
MDA-MB-435 M4naV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH7Jc4k1QCCq MnLxS2k:OC56ODFOwG0> MnmxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMUm1OVUoRjJ2MUG5OVU2RC:jPh?=
AsPC1 Mn\zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1ryRlMxKM7:TR?= M3SwblQ5KGh? MWjEUXNQ Mon4TWM2OD1|Lk[2JO69VQ>? NGGwVm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG4OVM4QCd-MkSxPFU{Pzh:L3G+
Jurkat E6.1 MYrLbY5ie2ViQYPzZZk> MnrZNkBp M3nrdmROW09? NEHsXoRKdmirYnn0bY9vKG:oIFjERWM1KHerdHigTWM2OCCxZjCwMlA1KM7:TR?= NITiTpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK2NVg3Oid-MkSyOlE5PjJ:L3G+
KG1 NIrjU4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{L5WVQ5KGh? MlTtTWM2OD1zLkG1JO69VQ>? NFz6UXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWyOVAxOyd-MkS1NlUxODN:L3G+
HEL NFnvWpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvsOFghcA>? NYSwO3RbTE2VTx?= NFXaWG1KSzVyPUCuOFkh|ryP MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ7NEC1OUc,OjR4OUSwOVU9N2F-
HSC3 MkTNR5l1d3SxeHnjJGF{e2G7 M{nVfFEzKGh? MoDVSG1UVw>? NX\w[VZYUUN3ME2wMlcyKM7:TR?= M{DWe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{[2OVYxLz5{NEe2OlU3ODxxYU6=
HT-29 NH;6b4hEgXSxdH;4bYMhSXO|YYm= NInTcm8yOiCq MXXEUXNQ MofSTWM2OD1yLkeyJO69VQ>? Ml;DQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5Nk[1OlAoRjJ2N{[2OVYxRC:jPh?=
KB MUXDfZRwfG:6aXOgRZN{[Xl? MYGxNkBp NHfNfZJFVVOR MoXGTWM2OD1yLke1JO69VQ>? M1fle|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{[2OVYxLz5{NEe2OlU3ODxxYU6=
H460 NUTEZYpzS3m2b4TvfIlkKEG|c3H5 M4\kVlEzKGh? NF;kWoxFVVOR NEKwPZdKSzVyPUCuPFIh|ryP MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4NkW2NEc,OjR5Nk[1OlA9N2F-
MONE1 MWfDfZRwfG:6aXOgRZN{[Xl? NHzLepgyOiCq NHrhPVNFVVOR MlHmTWM2OD1zLkG4JO69VQ>? NH3KbYQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2OlU3OCd-MkS3OlY2PjB:L3G+
MKN45 M{WzWGN6fG:2b4jpZ{BCe3OjeR?= NGr4cokyOiCq M2nTe2ROW09? NGXjVlVKSzVyPUGuNlUh|ryP NHf5cIY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2OlU3OCd-MkS3OlY2PjB:L3G+
TSGH M1HzZmN6fG:2b4jpZ{BCe3OjeR?= NVraRXBtOTJiaB?= NVfCdZdZTE2VTx?= NFywWmlKSzVyPUKuNFkh|ryP NVHLfINLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OlY2PjBpPkK0O|Y3PTZyPD;hQi=>
HL60 MlH0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX6wfXdEPzJiaB?= NGjqVINFVVOR MX3HTVUxRTBwOUOg{txO NXrlb2d3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA2ODBpPkK0PVAxPTByPD;hQi=>
HEK293 NHyxe|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoCzO|IhcA>? M33xcGROW09? NF3QWFdIUTVyPUGzJO69VQ>? NG[1WHk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFUxOCd-MkS5NFA2ODB:L3G+
Jurkat NH3jW21EgXSxdH;4bYMhSXO|YYm= NHWxTWcyODBizszN NHi2TZQ4OiCq MW\HTVUxRTBwOTFOwG0> Mki1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC3OFMoRjJ2OUCwO|Q{RC:jPh?=
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NCI-H2347 MnywS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFPXZ45KSzVyPUKzMlI3PzRizszN M{HLNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
U-118-MG NIS3SFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX23SoFMUUN3ME2yN{44OjF5IN88US=> M{XWSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H596 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlTnTWM2OD1{Mz65NFU2KM7:TR?= M3zPR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
MZ1-PC NYL1UGpHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWXJR|UxRTJ2Lk[3OVkh|ryP MmjJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
SW1417 MnPrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4O0NGlEPTB;MkWuNlQ6QCEQvF2= NXHuVVlbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
NCI-H82 NHXwPVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW\kcZR3UUN3ME2yOk41Ojh6IN88US=> M3G0bFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
COLO-678 M3TlcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRTJ5LkS5NVkh|ryP MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
KURAMOCHI NIPKdVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjCdJFKSzVyPUK4MlEzPiEQvF2= MnnuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NCI-H1650 MnzLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MljJTWM2OD1|MD6xPVI1KM7:TR?= NGrDR5o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
HCC1954 MlTNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWf5PYliUUN3ME2zNU4{PjZ4IN88US=> NUL0eoh2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
GAK NYXVSnhDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXTyUJNIUUN3ME2zNk4zPjF4IN88US=> NGPkSG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
YAPC MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnlTWM2OD1|Mz63OlQ5KM7:TR?= MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
NCI-H2228 NF:4fo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2rLZmlEPTB;M{SuN|YxPSEQvF2= NVjoXFY2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
VMRC-RCZ NHXwTFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnvzTWM2OD1|Nj6zNFIzKM7:TR?= NF[2e5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NMC-G1 MlOxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXrJR|UxRTN5LkC1Nlkh|ryP MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
D-336MG NVXtbW5[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUCwPZF7UUN3ME20NU45PDR{IN88US=> MonKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
UACC-62 NVq2TVczT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M17KT2lEPTB;NEKuOVUxPSEQvF2= MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
C-33-A MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml\VTWM2OD12NT6zNlU3KM7:TR?= NH23epQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
HCC1937 NUDoXHlsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTR5LkG5OVgh|ryP NY\GPY9kRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
HDLM-2 M1H3UGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGiwfFJKSzVyPUS3MlIzQDdizszN M4e1XFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H727 Ml7vS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTR5LkK0OVEh|ryP M3vNeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NY NFTzO2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYHX[4JzUUN3ME20PU4{QTB6IN88US=> Ml;TQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NCI-H2291 M{K3bmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPZXlFKUUN3ME20PU45OzBzIN88US=> Ml;YQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Ac-Histone H4 / Ac-Histone H3; 

PubMed: 21598070     


D54 and U87 glioma cells were treated with lower concentrations (1-3 µM) of vorinostat. The effects on histone acetylation was assessed by immunoblotting. 

phospho-CDK1; 

PubMed: 21598070     


Changes in level of inhibitory phosphorylation of cdk1 after treatment with vorinostat was determined at 24 and 48 h (using a Tyr-15 residue phospho-specific antibody) along with total cdk1 as loading control. 

phospho-Cdc25c / Cdc25c; 

PubMed: 21598070     


Time course of changes in phospho-cdc25c levels after exposure to vorinostat (5 µM).

Cyclin B1; 

PubMed: 21598070     


Cyclin B1 levels were determined in D54 and U87 cells after treatment with various doses of vorinostat at 24 and 48 h.

c-Myc / c-Raf / Akt / Cyclin D1 / CDK4; 

PubMed: 19440035     


Cell lysates from JeKo-1 and Mo2058 cells exposed to vorinostat  were immunoblotted for c-Myc, c-Raf, AKT, cyclin D1, CDK4 and acetylated histone H3. The levels of β-actin served as the loading control.

p-eIF2α / ATF4 / CHOP ; 

PubMed: 20072656     


Western blot was performed to evaluate activated eIF2α (P-eIF2α), translational up-regulation of ATF4, and CHOP expression in U251 cells after vorinostat exposure.

p21; 

PubMed: 21598070     


To determine the dose response relationship to vorinostat, cells were exposed to various higher concentrations (5–10 µM) of the agent and the levels of p21 determined by immunoblotting.

21598070 19440035 20072656
Immunofluorescence
HDAC1 / CK19; 

PubMed: 29917299     


Immunocytochemistry to detect the expression of HDAC1 and CK 19 in PDAC cells with/without Vorinostat treatment. HDAC1 and CK19 were stained red and green, respectively. The Hoechst‐stained nuclei appear blue. Scale bar: 100 μm. The CK19 expression in PSN1 and MiaPaCa2 was increased by Vorinostat treatment.

Nrf2; 

PubMed: 20566897     


Vorinostat induced translocation of Nrf2 from cytosol to nucleus. U937 cells were treated with 2μM vorinostat for 18 hours and then cytospun. Cells were fixed and labeled for Nrf2 protein (red) and nucleus (blue, 4,6-diamidino-2-phenylindole) as described in “Immunofluorescence and confocal microscopy.” Images were taken by Nikon Eclipse TE2000 confocal microscope with 40×/1.30 numeric aperture oil objective lens. Ctrl indicates control cells without treatment; and Vor, vorinostat.

α-SMA; 

PubMed: 26330748     


Representative immunofluorescence images and the quantification graph showing the effect of vorinostat (2.5 µM), TGIF1, and TGIF2 siRNA knockdown on αSMA. Control human corneal fibroblast cultures (-TGFβ) showed no αSMA staining. Cultures treated with TGFβ1 (5 ng/ml) showed a robust αSMA staining. Vorinostat treatment markedly attenuated TGFβ-evoked αSMA staining. The knockdown of TGIF1 (+TGFβ+vorinostat+TGIF1siRNA) and TGIF2 alone (+TGFβ+vorinostat+TGIF2siRNA) or in combination (+TGFβ +vorinostat +TGIF1 +2siRNA) markedly blocked the effect of vorinostat on αSMA. Scale bar=100 μm.

Ac-STAT3 / IGF2; 

PubMed: 27086926     


Immunofluorescence staining of Ac-STAT3 and IGF2 after vorinostat treatment.

29917299 20566897 26330748 27086926
Growth inhibition assay
Cell apoptosis; 

PubMed: 19440035     


Cells were treated with indicated concentrations of vorinostat for 48 hours. Following this, the percentage of annexin-V-stained apoptotic cells was determined by flow cytometry. Values represented as bar graphs are the mean of 3 experiments plus or minus the standard error (SE)

Cell viability; 

PubMed: 27086926     


The effects of vorinostat on the viability of the indicated NSCLC cell lines and their corresponding acquired VoR sublines. Cell viability was determined by the MTT assay (n = 3).

19440035 27086926
ELISA
IGF2; 

PubMed: 27086926     


Determination of vorinostat-induced IGF2 production by ELISA (n = 3). The conditioned mediums (CMs) obtained from cells treated with vorinostat (5 μM) for 2 days were used for ELISA. 

IL-13 / IL-10 / IL-5; 

PubMed: 18541724     


Effect of vorinostat on TH1/TH2 cytokine secretion in HL cell lines. Cells were incubated with medium or vorinostat (5 μM) for 24 hours and supernatants were examined for cytokine levels by ELISA. Each value represents a mean of 3 independent experiments done in triplicate (± SEM).

M-CSF; 

PubMed: 23042302     


Primary PyMT tumor cells were treated with SAHA (0-1000nM) for varying time points (8–48h), and supernatants were assayed by ELISA for M-CSF  secretion. *P < 0.05 and **P < 0.001 versus control treatment. 

MMP-9; 

PubMed: 23042302     


Primary PyMT tumor cells were treated with SAHA (0-1000nM) for varying time points (8–48h), and supernatants were assayed by ELISA for MMP-9 secretion. *P < 0.05 and **P < 0.001 versus control treatment. 

27086926 18541724 23042302
In vivo Administration of Vorinostat (~100 mg/kg/day) significantly inhibits the growth of CWR22 human prostate xenografts in nude mice with tumor reductions of 78%, 97% and 97%, at doses of 25 mg/kg/day, 50 mg/kg/day and 100 mg/kg/day, respectively, compared with control. Vorinostat induces the accumulation of acetylated core histones and prostate-specific antigen mRNA expression in CWR22 cells, resulting in higher levels of serum prostate-specific antigen than predicted from tumor volume alone. [2] Oral administration of Vorinostat (0.67g/L) crosses the blood-brain barrier, increases histone acetylation in the brain, and dramatically improves the motor impairment in the R6/2 mice model of Huntington's disease. [5]

Protocol

Kinase Assay:

[1]

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Immunoprecipitation-HDAC assays:

The lysate of Jurkat cells is incubated for 1 hour on ice and cleared by centrifugation at 12,000 g for 10 minutes at 4 °C. Supernatants are precleared with 30 μL of 50% protein G-Sepharose slurry for 1 hour at 4 °C. Beads are pelleted by centrifugation and supernatants are incubated for 1 hour at 4 °C with 10 μg of IgG fraction from anti-HDAC1 or HDAC3 polyclonal antisera (preincubated 2 hours at room temperature with either the homologous or heterologous immunizing peptide). Both antisera are raised in rabbits against the carboxylterminal peptide of HDAC1 and HDAC3 by using synthetic peptides coupled to keyhole limpet hemocyanin. 30 μL of 50% protein G-Sepharose slurry is added for 1 hour at 4 °C. Immune complexes are pelleted by centrifugation and washed three times with 1 mL of lysis buffer. Beads are resuspended in 200 μL of HDAC buffer (20 mM Tris-HCl, pH 8.0/150 mM NaCl/10% glycerol), and the HDAC assay is performed with an 3H-acetylated peptide corresponding to amino acids 1-24 of histone H4. Released [3H]acetic acid is quantified by scintillation counting. For inhibitions studies, the immunoprecipitated complexes are preincubated with the different concentrations of Vorinostat for 30 minutes at 4 °C.
Cell Research:

[2]

- Collapse
  • Cell lines: LNCaP, PC-3, and TSU-Pr1
  • Concentrations: Dissolved in DMSO, final concentrations ~7.5 μM
  • Incubation Time: 1, 2, 3 and 4 days
  • Method:

    Cells are exposed to various concentrations of Vorinostat for 1, 2, 3 and 4 days. Cell viability is assessed by trypan blue dye exclusion.


    (Only for Reference)
Animal Research:

[2]

- Collapse
  • Animal Models: Male BALB/c nude (nu/nu) mice implanted with CWR22 tumor cells
  • Dosages: ~100 mg/kg/day
  • Administration: Injection i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 52 mg/mL (196.74 mM)
Water Insoluble
Ethanol '''''3 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 264.3
Formula

C14H20N2O3

CAS No. 149647-78-9
Storage powder
in solvent
Synonyms MK0683, Suberoylanilide hydroxamic acid, Zolinza
Smiles C1=CC=C(C=C1)NC(=O)CCCCCCC(=O)NO

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Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03022565 Withdrawn Drug: Vorinostat Uveal Melanoma University of Miami|University of Miami Sylvester Comprehensive Cancer Center January 2020 Early Phase 1
NCT03167437 Recruiting Drug: Vorinostat|Drug: Ustekinumab Crohn''s Disease National Institute of Allergy and Infectious Diseases (NIAID)|National Institutes of Health Clinical Center (CC) October 30 2017 Phase 1|Phase 2
NCT03056495 Recruiting Drug: N-hydroxy-N''-phenyl-octanediamide (Vorinostat) Alzheimer Disease German Center for Neurodegenerative Diseases (DZNE)|University Hospital Bonn|University of Göttingen September 28 2017 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

  • Question 1:

    What’s the recommendation about reconstitution of the compound for in vivo animal study?

  • Answer:

    We recommend the following vehicle for S1047: 2% DMSO, 40% PEG 300, 5% Propylene glycol, 1% Tween 80 at 5 mg/mL.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID