Vorinostat (SAHA)

For research use only.

Catalog No.S1047 Synonyms: MK0683, Suberoylanilide hydroxamic acid

421 publications

Vorinostat (SAHA) Chemical Structure

CAS No. 149647-78-9

Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.

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Selleck's Vorinostat (SAHA) has been cited by 421 publications

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Choose Selective HDAC Inhibitors

Biological Activity

Description Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.
Targets
HDAC [1]
(Cell-free assay)
~10 nM
In vitro

Vorinostat inhibits the activities of HDAC1 and HDAC3 with IC50 of 10 nM and 20 nM, respectively. Vorinostat also results in a marked hyperacetylation of histone H4. [1] Vorinostat inhibits the growth of three prostate cancer cell lines LNCaP, PC-3 and TSU-Pr1 at micromolar concentrations (2.5-7.5 μM), and induces dose-dependent cell death in LNCaP cells. [2] Vorinostat treatment in MCF-7 cells inhibits cell proliferation at an IC50 of 0.75 μM resulting in the accumulation of cells in the G1 and G2-M phase of the cell cycle. Vorinostat also induces differentiation in the estrogen receptor-negative cell line SKBr-3 and the retinoblastoma-negative cell line MDA-468. [3] Vorinostat treatment at 1 μM for 8 hours or more is sufficient to irreversibly induce apoptosis of human multiple myeloma (MM) cells. The gene expression profiles of Vorinostat treated MM cells are not hallmarked by global transcriptional activation, but by coordinated transcriptional changes of specific functional groups of genes such as cytokine-induced proliferative/survival signaling cascades, oncogenes-tumor suppressor genes, regulators of apoptosis, DNA synthesis-repair and cell cycle, and proteasome-ubiquitin function. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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GM15850 NH;0RplMcW6jc3WgRZN{[Xl? NYrUSZE3Oi53IN88US=> M{DuPFk3KGh? M{XXb2ROW09? NX7JRnFkUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFQhdHm|aX7lJFUh[WOndInsZZRqd25ib3[gSnhPKGenbnW= NXLXR|RNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5NlE{PjdpPkG2PVIyOzZ5PD;hQi=>
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NCI-H2122 M1;3[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEXFO|Q4OiCq NX3qR2tpUUN3ME23MlUh|ryP NXjVe4FzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM4PzhpPkKwNVQ{Pzd6PD;hQi=>
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SKHEP1 MkLkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmP1O|IhcA>? M2fncGlEPTB;Mz60OkDPxE1? NVTxOHhRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM4PzhpPkKwNVQ{Pzd6PD;hQi=>
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Caco-2 NI\VPJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXixNFAh|ryP NIPBWG01QGh? NV3JdXJSTE2VTx?= NXLw[pZ6UUN3ME24MlEh|ryP NX[yXnBRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
Caco-2 NGLQPHpMcW6jc3WgRZN{[Xl? M{jUN|Uh|ryP NYPlPZZPPCCq NWC3[2hRTE2VTx?= MUTJcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDobZN1d26nIHHj[ZR6dGG2aX;uJGlEPTBib3[gNE4xOzdizszN Mlf0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
Caco-2 NEnKfWtMcW6jc3WgRZN{[Xl? MV[wMlMyPiCwTTD0c{A{OS54IN88US=> MlXlOEBp MmPsSG1UVw>? M2foV2V5KH[rdn:gbY5pcWKrdHnvckBw\iCqdX3hckBJTEGFMTD3bZRpKEmFNUCgc4YhOC5zMUmg{txO MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
Caco-2 M2\zcmtqdmG|ZTDBd5NigQ>? NYnyfYY3OC5|MU[gcm0hfG9iM{GuOkDPxE1? MmDSOEBp Ml35SG1UVw>? Mlv3SZghfmm4bzDpcohq[mm2aX;uJI9nKGi3bXHuJGhFSUN|IIfpeIghUUN3MDDv[kAxNjF2NzFOwG0> MoDCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
Caco-2 MkC5T4lv[XOnIFHzd4F6 NXXPT2h2Pi5|MjDuUUB1dyB4M{Kg{txO M4m1S|QhcA>? Ml\QSG1UVw>? M{\SS2V5KH[rdn:gbY5pcWKrdHnvckBw\iCqdX3hckBJTEGFNTD3bZRpKEmFNUCgc4YhOTJwNjFOwG0> M1ixNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzPFQxLz5{MEG0N|g1ODxxYU6=
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HepG2 NYnvfmM4U2mwYYPlJGF{e2G7 M2nyOlUh|ryP Ml3XOog> NFHUclJFVVOR MUHJcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDobZN1d26nIHHj[ZR6dGG2aX;u MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
HepG2 NF3hdHlMcW6jc3WgRZN{[Xl? M{GxS|Uh|ryP MlfEOog> NUXTd3dZTE2VTx?= M2LXUWlv\HWldHnvckBw\iCKRFHDMY1m\GmjdHXkJIhqe3SxbnWgbJlx\XKjY3X0fYxifGmxbh?= NUX5eWcyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
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NB4 M4LPT2tqdmG|ZTDBd5NigQ>? MVy1JO69VQ>? NHToVIMzPCCq M2[2cWROW09? M3;B[mlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDobZN1d26nIFizJIFk\XS7bHH0bY9v MmD4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2OUG0OFAoRjJyNEmxOFQxRC:jPh?=
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A549 MkXSR5l1d3SxeHnjJGF{e2G7 MoTiO|IhcA>? MkfER5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{BwfmW{ZYjwdoV{e2mwZzDISGFEKHerdHigTWM2OCCxZjCxMlgh|ryP MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
CAL27 Mki1R5l1d3SxeHnjJGF{e2G7 MkfCO|IhcA>? M3\2VWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNCVDJ5IHPlcIx{KG:4ZYLlfJBz\XO|aX7nJGVITlJid3n0bEBKSzVyIH;mJFMvOiEQvF2= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
HeLa MWrDfZRwfG:6aXOgRZN{[Xl? NVXLfXluPzJiaB?= MXvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJI93\XKneIDy[ZN{cW6pIFjERWMhf2m2aDDJR|UxKG:oIEGuOUDPxE1? Mn\UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2NlkoRjJzMEiwOlI6RC:jPh?=
Sf21 MX\LbY5ie2ViQYPzZZk> M1T1NVMhcA>? NIKyR|VKdmirYnn0bY9vKG:oIF\sZYchfGGpZ3XkJIh2dWGwIILlZ49u[mmwYX70JGhFSUN6IHX4dJJme3OnZDD3bZRpKEmFNUCgc4YhOS5{IN88US=> M136blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEiwOlI6Lz5{MUC4NFYzQTxxYU6=
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COR-L23 Mlu4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYOxNEDPxE1? NVvhOWptPzJiaB?= NYTrXWJvTE2VTx?= NYDzXZQ{T0l3ME2xMlEh|ryP MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[0O{c,OjFyOEC2OFc9N2F-
DU145 M{DlVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn;JNVAh|ryP M3[3V|czKGh? MYnEUXNQ NHnl[lRIUTVyPUGuNFUh|ryP MoPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2OFcoRjJzMEiwOlQ4RC:jPh?=
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HCT116 MVrLbY5ie2ViQYPzZZk> NFfQXmg{OCEQvF2= MX6zNEBp NYr5OoNRTE2VTx?= MVrJcohq[mm2aX;uJI9nKEiGQVOxJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDobZN1d26nIFizT|kh[WOndInsZZRqd25? NWOzNXY2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3PDdpPkKxNFgxPjR5PD;hQi=>
U937 NXHJSFRMU2mwYYPlJGF{e2G7 M2m2U|Uh|ryP M1fyVFI1KGh? NWnUPHZPTE2VTx?= MmfnTY5pcWKrdHnvckBw\iCqaYP0c45mKGGlZYT5cJRz[W6|ZnXyZZNmKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIH;mJIFk\XS7bHH0bY9vKG:oIHjpd5RwdmViSEOgZZQhUzl? NFzNfFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5NlQ6Oid-MkGyPVI1QTJ:L3G+
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U937 MoLyT4lv[XOnIFHzd4F6 NYL0do5rPSEQvF2= NHLBU|UzPCCq MW\EUXNQ MYnJcohq[mm2aX;uJI9nKGirc4TvcoUh[WOndInseJJidnOoZYLhd4Uh[XO|ZYPz[YQh[XNiaX7jdoVie2Vib3[gdIFvNWGlZYT5cIF1\WRiaHnzeI9v\SCKNDDs[ZZmdA>? NGDlPGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5NlQ6Oid-MkGyPVI1QTJ:L3G+
U937 NE\TNGFMcW6jc3WgRZN{[Xl? NIXqPIE2KM7:TR?= M1TSSVE2KG2rbh?= M4LmS2ROW09? NV;BcIhZUW6qaXLpeIlwdiCxZjDobZN1d26nIHHj[ZR6dHS{YX7z[oVz[XOnIHHzd4V{e2WmIHHzJIlv[3KnYYPlJI9nKGirc4TvcoUhUDNiYXPleJlt[XSrb36gcIV3\Wx? MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7MkS5Nkc,OjF{OUK0PVI9N2F-
A2780 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmC2PVYhcA>? Mme5SG1UVw>? NIDaTXlKSzVyPUGuOlIh|ryP MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTZ|NESzNEc,OjF4M{S0N|A9N2F-
COLO205 MmHyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LKRlk3KGh? NF33Z4pFVVOR MnT1TWM2OD1{LkGyJO69VQ>? NGHlNJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MU[zOFQ{OCd-MkG2N|Q1OzB:L3G+
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CFBE41o MX7LbY5ie2ViQYPzZZk> NV3LSJJCPSEQvF2= MX3EUXNQ MoXZSI94diC{ZXf1cIF1cW:wIH;mJGhFSUN5IHH0JFAvOiC2bzC1JJVO M2D6dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUi0PVU5Lz5{MUm4OFk2QDxxYU6=
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DU145 NGDxVpBMcW6jc3WgRZN{[Xl? MnHONk42KM7:TR?= NFLhUIszPCCq MXLEUXNQ MX7Jcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhfXC{ZXf1cIF1cW:wIH;mJJAzOXejZkGgdJJwfGWrbjDlfJBz\XO|aX;u M4DkUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[wNVY3Lz5{MkK2NFE3PjxxYU6=
DU145 MoHtT4lv[XOnIFHzd4F6 M4PZfVUh|ryP MkDtNlQhcA>? MojNSG1UVw>? MoXYTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGirc4TvcoUhUDRiYXPleJlt[XSrb36= NWrSTJpXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlAyPjZpPkKyNlYxOTZ4PD;hQi=>
DU145 NWf4[pd{U2mwYYPlJGF{e2G7 Mkj3OUDPxE1? NYeycm9vOjRiaB?= NWe3TYJ7TE2VTx?= MoKxTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGirc4TvcoUhUDNiaInw[ZJi[2W2eXzheIlwdg>? NV3Tb4VtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlAyPjZpPkKyNlYxOTZ4PD;hQi=>
786-0 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVywXIhKPi5{IN88US=> M17YXFczKGh? NX3sSJlpTE2VTx?= NVjqO4pOT0l3ME2zMlIh|ryP MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
A498 NWHtTJFxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1rIXFYvOiEQvF2= NIK4epQ4OiCq NEjWNpBFVVOR MVvHTVUxRTFwNjFOwG0> NUGwO|Y1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
A549/ATCC MmO5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVvEN3JbPi5{IN88US=> NYDDfFY6PzJiaB?= M1zWNWROW09? Mn3NS2k2OD1zLk[g{txO MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
ACHN MlHLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlnNOk4zKM7:TR?= M4DjTlczKGh? MnnLSG1UVw>? NIP1TpVIUTVyPUGuN{DPxE1? NVzKSGtiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
BT549 M2XsNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGf0XZc3NjJizszN NFPzcI44OiCq NXvxT|NDTE2VTx?= MoLWS2k2OD1zLkOg{txO MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
EKVX MmG0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVjpZVlEPi5{IN88US=> MlXNO|IhcA>? NWDVUnBwTE2VTx?= NEnNbI9IUTVyPUGuN{DPxE1? NYTodWdURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
HCT15 MlzMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUHZdFU2Pi5{IN88US=> NFWzOXY4OiCq M1zMe2ROW09? MkL5S2k2OD1{LkWg{txO NUezTJVQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
HOP92 NXj3bG9VT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmHnOk4zKM7:TR?= MlTFO|IhcA>? MkLpSG1UVw>? M1;3O2dKPTB;NDFOwG0> MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
IGROV1 NFjUelBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF3hb2Y3NjJizszN NU\zZmZ{PzJiaB?= Ml7SSG1UVw>? M2TCNmdKPTB;MT6zJO69VQ>? NFzzRXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
LOXIMVI M4rofWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVy2MlIh|ryP M1PHVlczKGh? M2nHeGROW09? NFn6U|hIUTVyPUGg{txO M4rMNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
M14 NXvPOYE2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkSwOk4zKM7:TR?= NVn4N49pPzJiaB?= NWPmWGpnTE2VTx?= MoXMS2k2OD1zLkOg{txO MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
MALME-3M NIT1VVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{OxfFYvOiEQvF2= MnG1O|IhcA>? NVPiN2ltTE2VTx?= M4T3emdKPTB;MD6zNkDPxE1? NXzyfIsxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
MCF10A Ml3oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUTNTolpPi5{IN88US=> NUnDWpBnPDhiaB?= MY\EUXNQ MlvKS2k2OD17Lk[g{txO M1mwWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
MDA-MB-435 Ml7KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYC2MlIh|ryP NGHSVXY4OiCq M2H5VGROW09? NF3CfWpIUTVyPUCuOUDPxE1? MlvEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
MDA-MB-468 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWG2MlIh|ryP MWK3NkBp M2XPS2ROW09? Mni2S2k2OD1yLk[zJO69VQ>? M{DqZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
MOLT4 MmPpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXG2MlIh|ryP M3\lOlczKGh? M2rLdWROW09? M4nXZmdKPTB;MD60JO69VQ>? MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
NCI-ADR-RES MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVS0ZphSPi5{IN88US=> MnvWO|IhcA>? MmWySG1UVw>? M4HUXGdKPTB;MD6xOkDPxE1? NH\qTWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
NCI-H23 M1nVSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVq2MlIh|ryP NFu3Zo04OiCq MWjEUXNQ NFr1RY9IUTVyPUGg{txO Ml3HQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
NCI-H322M MorsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEjvTWM3NjJizszN MkLNO|IhcA>? NIrpfndFVVOR MWLHTVUxRTBwN{mg{txO NGLKVYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
NCI-H460 MnjmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWq2MlIh|ryP NGLDfVU4OiCq MXzEUXNQ MlX2S2k2OD1yLke5JO69VQ>? M17hSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
NCI-H522 NYPrZ3hST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1jvcVYvOiEQvF2= MXe3NkBp NFfsO25FVVOR MmPKS2k2OD1yLkWg{txO NWnpN2hjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
OVCAR3 M2HFe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVTUUWFlPi5{IN88US=> MYO3NkBp NGrCWo9FVVOR NX\4OY92T0l3ME2xMlMh|ryP M2DwcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
OVCAR4 MlfkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVfaXGk{Pi5{IN88US=> MnGwO|IhcA>? NYPuUHFiTE2VTx?= NFHLOYJIUTVyPUSg{txO M1i3XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
OVCAR5 NHP3ToxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFm2Z2M3NjJizszN NG\YRlE4OiCq NU\yfnRkTE2VTx?= MmP5S2k2OD1yLke5JO69VQ>? M2T6VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
OVCAR8 NHi4TYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXK2MlIh|ryP MVG3NkBp NUX6UHNKTE2VTx?= NWP4N3RNT0l3ME2wMlUh|ryP MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
RXF393 MlHYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVj6VnQxPi5{IN88US=> MkT3O|IhcA>? MYnEUXNQ MoLaS2k2OD1zLkOg{txO NFXF[ZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
SF268 NHftS5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NG\rUHk3NjJizszN MknzO|IhcA>? MUTEUXNQ NXjiNIRHT0l3ME2xMlYh|ryP NXXRc|RNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
SF295 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUfDPG9tPi5{IN88US=> MXq3NkBp M2K0TmROW09? M{n1VGdKPTB;MT62JO69VQ>? MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
SF539 NVG5bIp1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvIcpVGPi5{IN88US=> NUL6OXJHPzJiaB?= MnnGSG1UVw>? MVrHTVUxRTJizszN MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
SK-MEL-2 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV3nN3gyPi5{IN88US=> NEHxb2o4OiCq NXvocZd5TE2VTx?= MV\HTVUxRTFwMzFOwG0> NIrCXos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
SK-MEL-28 NIDZO2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7oeXBYPi5{IN88US=> NF;m[WE4OiCq M174PWROW09? NX;QWGtLT0l3ME2xJO69VQ>? MlvOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
SK-MEL-5 NHLB[IRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnzpOk4zKM7:TR?= MW[3NkBp MYXEUXNQ MYDHTVUxRTBwNkOg{txO NGXDPGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
SKOV3 MlmyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4f3S|YvOiEQvF2= NWDCT|BqPzJiaB?= M1fmUGROW09? M33rZmdKPTB;MTFOwG0> Mn7mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
SN12C NV;zcpVbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWG2MlIh|ryP Ml\HO|IhcA>? NWPWTllyTE2VTx?= NXTQTYdZT0l3ME2yJO69VQ>? NWnzOoVTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
SNB19 M{DOd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXy5XHN6Pi5{IN88US=> NFHIN4Q4OiCq M2i2dWROW09? Ml3YS2k2OD1yLk[zJO69VQ>? NVLWTZN7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
SNB75 M2mzVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV3ocIliPi5{IN88US=> NFfLUm84OiCq NF23N3ZFVVOR NV2zU4l1T0l3ME2wMlc6KM7:TR?= NWP1OGlKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
SR M3HDXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\nXVYvOiEQvF2= NIrT[WY4OiCq M4LzUWROW09? NGLYfJFIUTVyPUCuOEDPxE1? NFr3RWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
T47D NW\pNXdvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFSzXlk3NjJizszN NGjSZWM4OiCq MlrRSG1UVw>? M4SzN2dKPTB;MD61JO69VQ>? MmPJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
TK10 MkXwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mmf1Ok4zKM7:TR?= Ml3jO|IhcA>? MlyxSG1UVw>? MXzHTVUxRTBwNkOg{txO MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
UACC257 NGDa[G1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4jDclYvOiEQvF2= MUG3NkBp NVrzNpViTE2VTx?= MYDHTVUxRTBwNTFOwG0> NIK5N5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
UACC62 MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV6yRVVWPi5{IN88US=> M2iwO|czKGh? MnPBSG1UVw>? Mn7ZS2k2OD1yLkSg{txO MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
UO31 M2TRd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXvUUo9IPi5{IN88US=> Mk\hO|IhcA>? NETOZlJFVVOR MYHHTVUxRTBwNTFOwG0> NEniSYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
Hep3B MoDWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmLJOFghcA>? M{DuO2ROW09? MUHHTVUxRTBwNkmg{txO M2PCZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO5PFY{Lz5{MkSzPVg3OzxxYU6=
BE(2)-C NX\0b3pOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWnUXXhOPzJiaB?= MkKySG1UVw>? NHjrPHdKSzdyPUKg{txO NHPJTZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmzNlMyPid-MkK5N|I{OTZ:L3G+
Bel7402 MnTwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M13JPVczKGh? NEL0SlRFVVOR MWPHTVkxRTF5IN88US=> MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MUO3Okc,OjNyNkGzO|Y9N2F-
Bel7404 Mlz3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVq3NkBp NHfzNnJFVVOR MojVS2k6OD1zODFOwG0> Mo\CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkGzO|YoRjJ|ME[xN|c3RC:jPh?=
BGC823 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1Hl[lczKGh? MYDEUXNQ NV\qR21ZT0l7ME23MlUh|ryP MlvVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkGzO|YoRjJ|ME[xN|c3RC:jPh?=
MGC803 M2XIUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXm3NkBp NUjlT2tETE2VTx?= NHTVeo5IUTlyPUGwMlgh|ryP MkXJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkGzO|YoRjJ|ME[xN|c3RC:jPh?=
SGC7901 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIrRSI04OiCq M3nDcGROW09? NYrTenFwT0l7ME25MlQh|ryP NIe2cFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
SKHEP1 NXS1ToRRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYHVXnpIPzJiaB?= NEXOZ2lFVVOR NWP5Z5E4T0l7ME2xO{DPxE1? M3v3NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|ME[xN|c3Lz5{M{C2NVM4PjxxYU6=
SMMC7721 M{LKNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlLWO|IhcA>? MWXEUXNQ MUfHTVkxRTJyIN88US=> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MUO3Okc,OjNyNkGzO|Y9N2F-
NUGC3 NIfVNnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVuxNEDPxE1? NFrxdmRIUT1{Lkm0JO69VQ>? NXX0XHFbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlM{OzJpPkKzNVY{OzN{PD;hQi=>
A2780 CisR MkDiT4lv[XOnIFHzd4F6 M{PRfFE5KGh? NF23RpRKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuOFQh|ryP MoDRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
CAL27 sens NGfvb|hMcW6jc3WgRZN{[Xl? MnH6NVghcA>? NGfLS5VKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuN|Yh|ryP MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
CAL27 CisR MYnLbY5ie2ViQYPzZZk> NHrUXHgyQCCq MV;Jcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNkGg{txO NE\rNWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
KYSE-510 CisR NX;nZZFDU2mwYYPlJGF{e2G7 MVuxPEBp NVHwbHNQUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlU6KM7:TR?= NUjjT3hZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
KYSE-510 sens NEXYW5pMcW6jc3WgRZN{[Xl? MmO4NVghcA>? MXjJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNzFOwG0> MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
MDA-MB-231 sens NHe3[IFMcW6jc3WgRZN{[Xl? Mmq2NVghcA>? MmnYTY5pcWKrdHnvckBw\iCKRFHDJJdqfGhiSVO1NEBw\iByLk[xJO69VQ>? NUDSPWlJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
MDA-MB-231 CisR Moj0T4lv[XOnIFHzd4F6 MoHaNVghcA>? NVz0[XBpUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlYyKM7:TR?= Ml;qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
ES2 M2LNNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlvsOFghcA>? MULEUXNQ NITrS5ZKSzVyPUGyMlch|ryP NVnrdYNRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVM1PDlpPkKzOFk{PDR7PD;hQi=>
MDA-MB-231 MV\LbY5ie2ViQYPzZZk> MlixNVAh|ryP NXPMWo81OzBibXnu NGrUTIZFVVOR MWnJcohq[mm2aX;uJI9nKEiGQVOyZS=> M37UflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NEmzOFQ6Lz5{M{S5N|Q1QTxxYU6=
MDA-MB-231 M3zjeGtqdmG|ZTDBd5NigQ>? MlzzNUDPxE1? MV:yOEBp NGL2XVJFVVOR NIr5WYlKdmirYnn0bY9vKG:oIFjERWM3KGG|c3Xzd4VlKGG|IHHj[ZR6dGG2aX;uJIxmfmWuczDv[kB1fWK3bHnu MnTPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUO0OFkoRjJ|NEmzOFQ6RC:jPh?=
HS68 MlfpR5l1d3SxeHnjJGF{e2G7 NFrLemUyOCEQvF2= NW\zNIhqPzJiaB?= NITtWWVFVVOR NWrKWoJ4UUN3ME20MlYh|ryP NV7JeFY6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1O|A2PDJpPkKzOVcxPTR{PD;hQi=>
MEF NXjZZ|RtS3m2b4TvfIlkKEG|c3H5 MV2xNEDPxE1? M3LzNVczKGh? MmHlSG1UVw>? MmHMTWM2OD12LkSg{txO NVz3NFJQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1O|A2PDJpPkKzOVcxPTR{PD;hQi=>
BGC823 M1;vfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1vaZ2ROW09? M3jMfWdKPTB;Mz6wPUDPxE1? M3vDdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkCxO|A3Lz5{M{[wNVcxPjxxYU6=
High5 MX3LbY5ie2ViQYPzZZk> M3fNVVI1KGh? NW\uZY1uUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEPiC5aYToJGlEPTBib3[gNE4xQSEQvF2= Mli3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4MEK1NlMoRjJ|NkCyOVI{RC:jPh?=
High5 M1eyemtqdmG|ZTDBd5NigQ>? M{jQRVI1KGh? MYDJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVOxJJdqfGhiSVO1NEBw\iByLkGyJO69VQ>? MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZyMkWyN{c,OjN4MEK1NlM9N2F-
High5 M3jUUGtqdmG|ZTDBd5NigQ>? MnvXNlQhcA>? MU\Jcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVOzJJdqfGhiSVO1NEBw\iByLkG3JO69VQ>? NVjNXXl7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NFI2OjNpPkKzOlAzPTJ|PD;hQi=>
A549 MkX1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3zJeGROW09? M{CwNWlEPTB;MT6wO|kh|ryP NGXZOI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[0OFIyOCd-MkO2OFQzOTB:L3G+
HCT116 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlPlSG1UVw>? MnvsTWM2OD1yLke1O{DPxE1? NWDIWml3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2OFQzOTBpPkKzOlQ1OjFyPD;hQi=>
K562 NYfre|VPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXfEUXNQ MoL4TWM2OD1yLk[0OUDPxE1? MkTKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NESyNVAoRjJ|NkS0NlExRC:jPh?=
GBC-SD M{TMcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW[1JO69VQ>? NHnUdHM4OiCq MnXzSG1UVw>? MUXJR|UxRTF2Lkig{txO MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyN{K2NEc,OjN5MEeyOlA9N2F-
HGC27 M{\PWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVS1JO69VQ>? NELwWHI4OiCq MW\EUXNQ NFvBdFdKSzVyPUWuOEDPxE1? NGnVNIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ewO|I3OCd-MkO3NFczPjB:L3G+
HL60 NH\EZYNMcW6jc3WgRZN{[Xl? MlfEOUDPxE1? NXrLOFlMOjRiaB?= MkjVSG1UVw>? M1frRmlvcGmkaYTpc44hd2ZiSFTBRy=> NHzHd3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ewO|I3OCd-MkO3NFczPjB:L3G+
Vero MmLqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXm3NkBp NWPk[Is3TE2VTx?= M{TKbGlEPTB;MD65JO69VQ>? NFrISpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e4OlQ2Oid-MkO3PFY1PTJ:L3G+
NCI60 NVnqSZhXS3m2b4TvfIlkKEG|c3H5 MnvmOFghcA>? M{XGVGROW09? M3jVSGdKRTBwNUOg{txO MoHqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUWzNlcoRjJ2MEG1N|I4RC:jPh?=
SW620 MY\DfZRwfG:6aXOgRZN{[Xl? MkTGOFghcA>? MlPOSG1UVw>? NYWwWJZGT0l;MD61OEDPxE1? M1X0S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEG1N|I4Lz5{NECxOVMzPzxxYU6=
U251 M3PKbGN6fG:2b4jpZ{BCe3OjeR?= NXLlZVI{PDhiaB?= Mm\1SG1UVw>? MVvHTV0yNjV|IN88US=> MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDBzNUOyO{c,OjRyMUWzNlc9N2F-
KM12 M4XRdWN6fG:2b4jpZ{BCe3OjeR?= MmjGOFghcA>? NHHwXVlFVVOR NYHxRWJoT0l;MT64PEDPxE1? NEXz[GE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECxOVMzPyd-MkSwNVU{Ojd:L3G+
Hs578T M3TPfWN6fG:2b4jpZ{BCe3OjeR?= NHSxe5Y1QCCq NGP1bmhFVVOR M1zCXWdKRTRwOEOg{txO M{mwNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEG1N|I4Lz5{NECxOVMzPzxxYU6=
MDA-MB-231 NWHOS|ZOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjxR2M1QCCq M1;leWlEPTB;MD6xOkDPxE1? MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7NUCxOkc,OjRyOUWwNVY9N2F-
MCF7 NH7nTolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX:0PEBp MWTHTV0xNjR3IN88US=> NVn0TItERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNVk2PTVpPkK0NVE6PTV3PD;hQi=>
MDA-MB-435 NY[5fWxST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHPuTYQ1QCCq MkTMS2k:OC56ODFOwG0> NV[yWVJzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNVk2PTVpPkK0NVE6PTV3PD;hQi=>
AsPC1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXezNEDPxE1? MlLTOFghcA>? MUHEUXNQ MkfVTWM2OD1|Lk[2JO69VQ>? MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF6NUO3PEc,OjRzOEWzO|g9N2F-
Jurkat E6.1 M{\NW2tqdmG|ZTDBd5NigQ>? NXXZfYNNOiCq NH2wTnBFVVOR NWHCTJZ[UW6qaXLpeIlwdiCxZjDISGFEPCC5aYToJGlEPTBib3[gNE4xPCEQvF2= NVnNOpV{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOlE5PjJpPkK0NlYyQDZ{PD;hQi=>
KG1 NF\0TolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3jwcFQ5KGh? NGr1NYFKSzVyPUGuNVUh|ryP MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV{NUCwN{c,OjR3MkWwNFM9N2F-
HEL MkTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUS0PEBp MorUSG1UVw>? NInWNIZKSzVyPUCuOFkh|ryP MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ7NEC1OUc,OjR4OUSwOVU9N2F-
HSC3 MXXDfZRwfG:6aXOgRZN{[Xl? MlHlNVIhcA>? NGHNTnVFVVOR MmPoTWM2OD1yLkexJO69VQ>? NIDicJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2OlU3OCd-MkS3OlY2PjB:L3G+
HT-29 MY\DfZRwfG:6aXOgRZN{[Xl? NYrQTlhGOTJiaB?= NVnUXWF5TE2VTx?= M1;zbGlEPTB;MD63NkDPxE1? MoHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5Nk[1OlAoRjJ2N{[2OVYxRC:jPh?=
KB NXHSSYE2S3m2b4TvfIlkKEG|c3H5 M{LOVlEzKGh? MlzoSG1UVw>? MknMTWM2OD1yLke1JO69VQ>? MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4NkW2NEc,OjR5Nk[1OlA9N2F-
H460 MlP4R5l1d3SxeHnjJGF{e2G7 M1HPWFEzKGh? MVrEUXNQ NXTtdo1EUUN3ME2wMlgzKM7:TR?= MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4NkW2NEc,OjR5Nk[1OlA9N2F-
MONE1 MYPDfZRwfG:6aXOgRZN{[Xl? Ml\KNVIhcA>? NWi2e5dPTE2VTx?= NYLmVWt3UUN3ME2xMlE5KM7:TR?= NYHxWWtuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OlY2PjBpPkK0O|Y3PTZyPD;hQi=>
MKN45 M2HkPGN6fG:2b4jpZ{BCe3OjeR?= Mne4NVIhcA>? NUnuNGQ1TE2VTx?= NUKzOG56UUN3ME2xMlI2KM7:TR?= NWDub3I5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OlY2PjBpPkK0O|Y3PTZyPD;hQi=>
TSGH NGX3[3ZEgXSxdH;4bYMhSXO|YYm= MV6xNkBp M37RfWROW09? MVHJR|UxRTJwMEmg{txO MnXJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5Nk[1OlAoRjJ2N{[2OVYxRC:jPh?=
HL60 Mn6zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkjlO|IhcA>? MUjEUXNQ MV;HTVUxRTBwOUOg{txO NVnxVJZQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA2ODBpPkK0PVAxPTByPD;hQi=>
HEK293 M33qZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnP0O|IhcA>? NET0[XNFVVOR M{\yV2dKPTB;MUOg{txO Mlf6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC1NFAoRjJ2OUCwOVAxRC:jPh?=
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SK-LU-1 M{HYOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;NNZJlUUN3ME2yN{4xPzF6IN88US=> NH;EV3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
UACC-893 M2Pmd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTJ|LkGwN|gh|ryP NWnzeng3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
NCI-H2347 MoTyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkPmTWM2OD1{Mz6yOlc1KM7:TR?= NGGwT4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
U-118-MG MmH4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXfsU5Y5UUN3ME2yN{44OjF5IN88US=> NGLOPIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NCI-H596 NXXne4tGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTJ|LkmwOVUh|ryP NETnWpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
MZ1-PC NV;xeZY5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkP2TWM2OD1{ND62O|U6KM7:TR?= MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
SW1417 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGO5RXBKSzVyPUK1MlI1QThizszN Mmj3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NCI-H82 MlvLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWexXIVrUUN3ME2yOk41Ojh6IN88US=> NU\yZoptRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
COLO-678 M4rkRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFP2O5JKSzVyPUK3MlQ6OTlizszN NVS5OnNyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
KURAMOCHI M1PGUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mo\pTWM2OD1{OD6xNlYh|ryP NHPrOlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NCI-H1650 NEXzUm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWnJR|UxRTNyLkG5NlQh|ryP NGrIVmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
HCC1954 NF\ocItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2q0cmlEPTB;M{GuN|Y3PiEQvF2= MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
GAK M1;GPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTN{LkK2NVYh|ryP NIfi[nQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
YAPC M13hXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHPeJJyUUN3ME2zN{44PjR6IN88US=> NYH3dWxDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
NCI-H2228 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\U[mlEPTB;M{SuN|YxPSEQvF2= M3jCdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
VMRC-RCZ NXvH[nJLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MULJR|UxRTN4LkOwNlIh|ryP M3ywclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NMC-G1 M2rOXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvYNGpKSzVyPUO3MlA2OjlizszN M2KzeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
D-336MG MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHmyPY9KSzVyPUSxMlg1PDJizszN MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
UACC-62 MmG0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3L1WmlEPTB;NEKuOVUxPSEQvF2= NHj6d5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
C-33-A Ml24S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXvJR|UxRTR3LkOyOVYh|ryP M{LFdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
HCC1937 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4LI[2lEPTB;NEeuNVk2QCEQvF2= M3mwS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
HDLM-2 NUXhbplMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV;JR|UxRTR5LkKyPFch|ryP MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
NCI-H727 MmrSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTR5LkK0OVEh|ryP MlfOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NY MnjHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGr0NZJKSzVyPUS5MlM6ODhizszN NUnlPWZRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
NCI-H2291 M4TJSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYrJR|UxRTR7LkizNFEh|ryP NILUeIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Ac-Histone H4 / Ac-Histone H3; 

PubMed: 21598070     


D54 and U87 glioma cells were treated with lower concentrations (1-3 µM) of vorinostat. The effects on histone acetylation was assessed by immunoblotting. 

phospho-CDK1; 

PubMed: 21598070     


Changes in level of inhibitory phosphorylation of cdk1 after treatment with vorinostat was determined at 24 and 48 h (using a Tyr-15 residue phospho-specific antibody) along with total cdk1 as loading control. 

phospho-Cdc25c / Cdc25c; 

PubMed: 21598070     


Time course of changes in phospho-cdc25c levels after exposure to vorinostat (5 µM).

Cyclin B1; 

PubMed: 21598070     


Cyclin B1 levels were determined in D54 and U87 cells after treatment with various doses of vorinostat at 24 and 48 h.

c-Myc / c-Raf / Akt / Cyclin D1 / CDK4; 

PubMed: 19440035     


Cell lysates from JeKo-1 and Mo2058 cells exposed to vorinostat  were immunoblotted for c-Myc, c-Raf, AKT, cyclin D1, CDK4 and acetylated histone H3. The levels of β-actin served as the loading control.

p-eIF2α / ATF4 / CHOP ; 

PubMed: 20072656     


Western blot was performed to evaluate activated eIF2α (P-eIF2α), translational up-regulation of ATF4, and CHOP expression in U251 cells after vorinostat exposure.

p21; 

PubMed: 21598070     


To determine the dose response relationship to vorinostat, cells were exposed to various higher concentrations (5–10 µM) of the agent and the levels of p21 determined by immunoblotting.

21598070 19440035 20072656
Immunofluorescence
HDAC1 / CK19; 

PubMed: 29917299     


Immunocytochemistry to detect the expression of HDAC1 and CK 19 in PDAC cells with/without Vorinostat treatment. HDAC1 and CK19 were stained red and green, respectively. The Hoechst‐stained nuclei appear blue. Scale bar: 100 μm. The CK19 expression in PSN1 and MiaPaCa2 was increased by Vorinostat treatment.

Nrf2; 

PubMed: 20566897     


Vorinostat induced translocation of Nrf2 from cytosol to nucleus. U937 cells were treated with 2μM vorinostat for 18 hours and then cytospun. Cells were fixed and labeled for Nrf2 protein (red) and nucleus (blue, 4,6-diamidino-2-phenylindole) as described in “Immunofluorescence and confocal microscopy.” Images were taken by Nikon Eclipse TE2000 confocal microscope with 40×/1.30 numeric aperture oil objective lens. Ctrl indicates control cells without treatment; and Vor, vorinostat.

α-SMA; 

PubMed: 26330748     


Representative immunofluorescence images and the quantification graph showing the effect of vorinostat (2.5 µM), TGIF1, and TGIF2 siRNA knockdown on αSMA. Control human corneal fibroblast cultures (-TGFβ) showed no αSMA staining. Cultures treated with TGFβ1 (5 ng/ml) showed a robust αSMA staining. Vorinostat treatment markedly attenuated TGFβ-evoked αSMA staining. The knockdown of TGIF1 (+TGFβ+vorinostat+TGIF1siRNA) and TGIF2 alone (+TGFβ+vorinostat+TGIF2siRNA) or in combination (+TGFβ +vorinostat +TGIF1 +2siRNA) markedly blocked the effect of vorinostat on αSMA. Scale bar=100 μm.

Ac-STAT3 / IGF2; 

PubMed: 27086926     


Immunofluorescence staining of Ac-STAT3 and IGF2 after vorinostat treatment.

29917299 20566897 26330748 27086926
Growth inhibition assay
Cell apoptosis; 

PubMed: 19440035     


Cells were treated with indicated concentrations of vorinostat for 48 hours. Following this, the percentage of annexin-V-stained apoptotic cells was determined by flow cytometry. Values represented as bar graphs are the mean of 3 experiments plus or minus the standard error (SE)

Cell viability; 

PubMed: 27086926     


The effects of vorinostat on the viability of the indicated NSCLC cell lines and their corresponding acquired VoR sublines. Cell viability was determined by the MTT assay (n = 3).

19440035 27086926
ELISA
IGF2; 

PubMed: 27086926     


Determination of vorinostat-induced IGF2 production by ELISA (n = 3). The conditioned mediums (CMs) obtained from cells treated with vorinostat (5 μM) for 2 days were used for ELISA. 

IL-13 / IL-10 / IL-5; 

PubMed: 18541724     


Effect of vorinostat on TH1/TH2 cytokine secretion in HL cell lines. Cells were incubated with medium or vorinostat (5 μM) for 24 hours and supernatants were examined for cytokine levels by ELISA. Each value represents a mean of 3 independent experiments done in triplicate (± SEM).

M-CSF; 

PubMed: 23042302     


Primary PyMT tumor cells were treated with SAHA (0-1000nM) for varying time points (8–48h), and supernatants were assayed by ELISA for M-CSF  secretion. *P < 0.05 and **P < 0.001 versus control treatment. 

MMP-9; 

PubMed: 23042302     


Primary PyMT tumor cells were treated with SAHA (0-1000nM) for varying time points (8–48h), and supernatants were assayed by ELISA for MMP-9 secretion. *P < 0.05 and **P < 0.001 versus control treatment. 

27086926 18541724 23042302
In vivo Administration of Vorinostat (~100 mg/kg/day) significantly inhibits the growth of CWR22 human prostate xenografts in nude mice with tumor reductions of 78%, 97% and 97%, at doses of 25 mg/kg/day, 50 mg/kg/day and 100 mg/kg/day, respectively, compared with control. Vorinostat induces the accumulation of acetylated core histones and prostate-specific antigen mRNA expression in CWR22 cells, resulting in higher levels of serum prostate-specific antigen than predicted from tumor volume alone. [2] Oral administration of Vorinostat (0.67g/L) crosses the blood-brain barrier, increases histone acetylation in the brain, and dramatically improves the motor impairment in the R6/2 mice model of Huntington's disease. [5]

Protocol

Kinase Assay:

[1]

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Immunoprecipitation-HDAC assays:

The lysate of Jurkat cells is incubated for 1 hour on ice and cleared by centrifugation at 12,000 g for 10 minutes at 4 °C. Supernatants are precleared with 30 μL of 50% protein G-Sepharose slurry for 1 hour at 4 °C. Beads are pelleted by centrifugation and supernatants are incubated for 1 hour at 4 °C with 10 μg of IgG fraction from anti-HDAC1 or HDAC3 polyclonal antisera (preincubated 2 hours at room temperature with either the homologous or heterologous immunizing peptide). Both antisera are raised in rabbits against the carboxylterminal peptide of HDAC1 and HDAC3 by using synthetic peptides coupled to keyhole limpet hemocyanin. 30 μL of 50% protein G-Sepharose slurry is added for 1 hour at 4 °C. Immune complexes are pelleted by centrifugation and washed three times with 1 mL of lysis buffer. Beads are resuspended in 200 μL of HDAC buffer (20 mM Tris-HCl, pH 8.0/150 mM NaCl/10% glycerol), and the HDAC assay is performed with an 3H-acetylated peptide corresponding to amino acids 1-24 of histone H4. Released [3H]acetic acid is quantified by scintillation counting. For inhibitions studies, the immunoprecipitated complexes are preincubated with the different concentrations of Vorinostat for 30 minutes at 4 °C.
Cell Research:

[2]

- Collapse
  • Cell lines: LNCaP, PC-3, and TSU-Pr1
  • Concentrations: Dissolved in DMSO, final concentrations ~7.5 μM
  • Incubation Time: 1, 2, 3 and 4 days
  • Method:

    Cells are exposed to various concentrations of Vorinostat for 1, 2, 3 and 4 days. Cell viability is assessed by trypan blue dye exclusion.


    (Only for Reference)
Animal Research:

[2]

- Collapse
  • Animal Models: Male BALB/c nude (nu/nu) mice implanted with CWR22 tumor cells
  • Dosages: ~100 mg/kg/day
  • Administration: Injection i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 52 mg/mL (196.74 mM)
Water Insoluble
Ethanol '''3 mg/mL warmed
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 264.3
Formula

C14H20N2O3

CAS No. 149647-78-9
Storage powder
in solvent
Synonyms MK0683, Suberoylanilide hydroxamic acid
Smiles C1=CC=C(C=C1)NC(=O)CCCCCCC(=O)NO

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Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03022565 Withdrawn Drug: Vorinostat Uveal Melanoma University of Miami|University of Miami Sylvester Comprehensive Cancer Center January 2020 Early Phase 1
NCT03167437 Recruiting Drug: Vorinostat|Drug: Ustekinumab Crohn''s Disease National Institute of Allergy and Infectious Diseases (NIAID)|National Institutes of Health Clinical Center (CC) October 30 2017 Phase 1|Phase 2
NCT03056495 Recruiting Drug: N-hydroxy-N''-phenyl-octanediamide (Vorinostat) Alzheimer Disease German Center for Neurodegenerative Diseases (DZNE)|University Hospital Bonn|University of Göttingen September 28 2017 Phase 1
NCT04308330 Recruiting Drug: Vorinostat Ewing Sarcoma|Rhabdomyosarcoma|Wilms Tumor|Neuroblastoma|Hepatoblastoma|Germ Cell Tumor New York Medical College March 17 2017 Phase 1
NCT02836548 Unknown status Drug: Vorinostat Melanoma|Skin Neoplasms The Netherlands Cancer Institute June 2016 Phase 1|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

  • Question 1:

    What’s the recommendation about reconstitution of the compound for in vivo animal study?

  • Answer:

    We recommend the following vehicle for S1047: 2% DMSO, 40% PEG 300, 5% Propylene glycol, 1% Tween 80 at 5 mg/mL.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID