Vorinostat (SAHA)

For research use only.

Catalog No.S1047 Synonyms: MK0683, Suberoylanilide hydroxamic acid

441 publications

Vorinostat (SAHA) Chemical Structure

CAS No. 149647-78-9

Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.

Selleck's Vorinostat (SAHA) has been cited by 441 publications

Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.
Targets
HDAC [1]
(Cell-free assay)
~10 nM
In vitro

Vorinostat inhibits the activities of HDAC1 and HDAC3 with IC50 of 10 nM and 20 nM, respectively. Vorinostat also results in a marked hyperacetylation of histone H4. [1] Vorinostat inhibits the growth of three prostate cancer cell lines LNCaP, PC-3 and TSU-Pr1 at micromolar concentrations (2.5-7.5 μM), and induces dose-dependent cell death in LNCaP cells. [2] Vorinostat treatment in MCF-7 cells inhibits cell proliferation at an IC50 of 0.75 μM resulting in the accumulation of cells in the G1 and G2-M phase of the cell cycle. Vorinostat also induces differentiation in the estrogen receptor-negative cell line SKBr-3 and the retinoblastoma-negative cell line MDA-468. [3] Vorinostat treatment at 1 μM for 8 hours or more is sufficient to irreversibly induce apoptosis of human multiple myeloma (MM) cells. The gene expression profiles of Vorinostat treated MM cells are not hallmarked by global transcriptional activation, but by coordinated transcriptional changes of specific functional groups of genes such as cytokine-induced proliferative/survival signaling cascades, oncogenes-tumor suppressor genes, regulators of apoptosis, DNA synthesis-repair and cell cycle, and proteasome-ubiquitin function. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H1299 M4fo[WtqdmG|ZTDBd5NigQ>? NGDU[HkzOCCvaX6= M3fWXGROW09? NVvjbmJpUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KEiGQVOg[Y57gW2nIIfpeIghUUN3MDDv[kAxNjF7NDFOwG0> NVuxZndHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS1NlE1OjJpPkG0OVIyPDJ{PD;hQi=>
HT1080 M3HK[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPqcG9QPzJiaB?= NY\4NW9TTE2VTx?= NHjkO2pKSzVyPUKuOEDPxE1? MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjJ5MEG3OUc,OTJ{N{CxO|U9N2F-
K562 MVXLbY5ie2ViQYPzZZk> MnfnOkBp M1XBZmROW09? M4PGVGlvcGmkaYTpc44hd2ZiaIXtZY4hcGm|dH;u[UBl\WGlZYT5cIF{\SBqbXn4eJVz\SCxZjDISGFEOSCjbnSgTGRCSzJrIIfpeIghUUN3MDDv[kAxNjBzIN88US=> Mk\NQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ2MUmzPFAoRjF{NEG5N|gxRC:jPh?=
A20 NEnxUWZMcW6jc3WgRZN{[Xl? NVzPN4pJOSCq MUXEUXNQ MWThcpRqNUiGQVOxJIFkfGm4aYT5JJdqfGhiSVO1NEBw\iByLkGxNkDPxE1? M{noPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOU[wOFg6Lz5zMUm2NFQ5QTxxYU6=
Friend leukemic cell NFi1eXNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1X0XVUxKM7:TR?= M1fzclczKGh? MkGxSG1UVw>? NFvrOXdKSzVyPUCuPVkh|ryP MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjFyOUmxN{c,OTJzMEm5NVM9N2F-
H1299 MorBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVrQdIp6PzJiaB?= MnTTSG1UVw>? MlT2TWM2OD15LkK0JO69VQ>? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDV{MUSyNkc,OTR3MkG0NlI9N2F-
HeLa NEnuRWJMcW6jc3WgRZN{[Xl? NETzfWg{OCCvaX6= NUPqOFJrUW6qaXLpeI9zgSClb37j[Y51emG2aX;uJIFo[Wmwc4SgbIl{fG:wZTDk[YFk\XS7bHHz[UB4cXSqIFnDOVAhd2ZiMD6yPEDPxE1? NF3r[pE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNU[wN|k1QSd-MUW2NFM6PDl:L3G+
LOX-IMVI NE\rcY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXy0PEBp NEXlcYtKSzVyPUGuN{DPxE1? MljWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5MUW0O|AoRjF3N{G1OFcxRC:jPh?=
NCI-H226 NEfOSGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn7wOFghcA>? NEHYT|NKSzVyPUKuOkDPxE1? NX;vRXlpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3NVU1PzBpPkG1O|E2PDdyPD;hQi=>
RXF-631L MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkO1OFghcA>? MkXKTWM2OD1{IN88US=> NVzSWVR3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3NVU1PzBpPkG1O|E2PDdyPD;hQi=>
SNB-78 Ml\NS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW[0PEBp MVfJR|UxRTF4IN88US=> NYjOfIl2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3NVU1PzBpPkG1O|E2PDdyPD;hQi=>
St-4 M3rSO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXz6dGZxPDhiaB?= MlfoTWM2OD13LkKg{txO M3;PdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{G1OFcxLz5zNUexOVQ4ODxxYU6=
ARP1 NWLhOXZsTnWwY4Tpc44hSXO|YYm= NWOzS4p7OiEQvF2= MWi2JIg> NHTBRmdKdmS3Y4Tpc44hd2ZiUEKxW2FHOSCpZX7lJIV5eHKnc4Ppc44> NXGzU5RIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS3N|Q5ODZpPkG0O|M1QDB4PD;hQi=>
CEM M4j5OWtqdmG|ZTDBd5NigQ>? NIfxN48yKGh? NEXYdZVFVVOR NH3sW2ZKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuN|Mh|ryP MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjR{MEC2OEc,OTZ2MkCwOlQ9N2F-
HCT15 MoXrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;EPXI1QCCq NH;n[olIUTVyPUCuPFIh|ryP MV68ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjd{M{KyO{c,OTZ5MkOyNlc9N2F-
NCI-H23 NX7XUGNPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnG2OFghcA>? NHy1c4xIUTVyPUCuPVIh|ryP NXXxNlZoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[3NlMzOjdpPkG2O|I{OjJ5PD;hQi=>
GM15850 Mnv2T4lv[XOnIFHzd4F6 NHTPRVkzNjVizszN M2rtZ|k3KGh? M3nTcGROW09? NY\HZnM3UW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFMhdHm|aX7lJFE1KGGlZYT5cIF1cW:wIH;mJGZZViCpZX7l MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl{MUO2O{c,OTZ7MkGzOlc9N2F-
GM15850 MX;LbY5ie2ViQYPzZZk> NHnGbXozNjVizszN M1PINFk3KGh? MoWwSG1UVw>? NYHPcHpyUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFQhdHm|aX7lJFUh[WOndInsZZRqd25ib3[gSnhPKGenbnW= NWfVUlllRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5NlE{PjdpPkG2PVIyOzZ5PD;hQi=>
GM15850 MWPLbY5ie2ViQYPzZZk> NH7XVYMzNjVizszN Mm[wPVYhcA>? MoLUSG1UVw>? NV7sfmtRUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFQhdHm|aX7lJFEzKGGlZYT5cIF1cW:wIH;mJGZZViCpZX7l NFnRSmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmyNVM3Pyd-MU[5NlE{Pjd:L3G+
GM15850 M1S4V2tqdmG|ZTDBd5NigQ>? MnXKNk42KM7:TR?= NH34OJA6PiCq NFvGN4JFVVOR MlTTTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViSEOgcJl{cW6nIEmgZYNmfHmuYYTpc44hd2ZiRmjOJIdmdmV? M4nGeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUKxN|Y4Lz5zNkmyNVM3PzxxYU6=
GM15850 NFTNdXlMcW6jc3WgRZN{[Xl? M3LQWVIvPSEQvF2= MoXMPVYhcA>? NEXyS3pFVVOR MkH1TY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViSESgcJl{cW6nIEigZYNmfHmuYYTpc44hd2ZiRmjOJIdmdmV? NHHHVpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNkmyNVM3Pyd-MU[5NlE{Pjd:L3G+
GM15850 NIHveHJMcW6jc3WgRZN{[Xl? M{K4TFIvPSEQvF2= MV:5OkBp MnXlSG1UVw>? M3j2VWlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDobZN1d26nIFi0JIx6e2mwZTCxOkBi[2W2eXzheIlwdiCxZjDGXG4h\2WwZR?= NYfQe2JTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5NlE{PjdpPkG2PVIyOzZ5PD;hQi=>
U937 MnrTSpVv[3Srb36gRZN{[Xl? MVi1JO69VQ>? NWjTUHlpOjRiaB?= MXzEUXNQ NF3iWoFE\WyuIHP5Z4xmKGG{cnXzeEBjgSCjY3P1cZVt[XSrb36gZZQhWy:JMj;NJJBp[XOn NWryT5hRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUewNFQ4OThpPkG3NFA1PzF6PD;hQi=>
H661 MnPZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;nOHF6PDhiaB?= MX\JR|UxRTFizszN Ml7lQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzNUewNFkoRjF5MUW3NFA6RC:jPh?=
U937 M2r4R2Z2dmO2aX;uJGF{e2G7 NXj2SYlmPSEQvF2= MoO1NlQhcA>? MWTEUXNQ NY\abZlwUW6lcnXhd4UhcW5iaHnzeI9v\SCKMzDhZ4V1gWyjdHnvci=> NEHFNGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S0O|c2OCd-MUe0OFc4PTB:L3G+
U937 M3;QNWZ2dmO2aX;uJGF{e2G7 M4TSclEh|ryP MViyOEBp MUjEUXNQ NEe2SmZG\m[nY4Sgc44h[WyyaHGgeJVjfWyrbjDhZ4V1gWyjdHnvci=> NYfwVXFzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OFc4PTBpPkG3OFQ4PzVyPD;hQi=>
RAW264.7 NG\lWo1HfW6ldHnvckBCe3OjeR?= NFzpV3kyOCEQvF2= NWW2TFdUOjSq M{THV5difGW{ M4L4fWlvcGmkaYTpc44hd2ZiTGDTMZN1cW23bHH0[YQhXE6IYXzwbIEheHKxZIXjeIlwdiC5aYToJGlEPTBib3[gNE44PiEQvF2= MnLsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2N{e1NVgoRjF5NEe3OVE5RC:jPh?=
RAW264.7 NWj4[GxXTnWwY4Tpc44hSXO|YYm= M3nvcVExKM7:TR?= MoP5NlRp Mli0e4F1\XJ? M4LEZ2lvcGmkaYTpc44hd2ZiTGDTMZN1cW23bHH0[YQhVk9icILv[JVkfGmxbjD3bZRpKEmFNUCgc4YhOS56ODFOwG0> MlTNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2N{e1NVgoRjF5NEe3OVE5RC:jPh?=
SC9 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoG2OFghcA>? NEXEZo1KSzVyPUCuOlA3KM7:TR?= MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVyN{KxPUc,OTd3MEeyNVk9N2F-
ST14A NXfrPWk1TnWwY4Tpc44hSXO|YYm= MmfkOUBvVQ>? M{XGTlczKGh? NFnybmFKdmO{ZXHz[UBqdiCURWPUM25TW0ZibWLORUBmgHC{ZYPzbY9v NELvUFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W2OVk6Oyd-MUe1OlU6QTN:L3G+
ST14A NGC1SlFHfW6ldHnvckBCe3OjeR?= Ml7hOUBvVQ>? NHnKblY4OiCq Mlr2TY5kemWjc3WgbY4hTTKIMTDtVm5CKGW6cILld5Nqd25? NImzdpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W2OVk6Oyd-MUe1OlU6QTN:L3G+
PANC1 NGrqN2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWKxJO69VQ>? MWDJR|UxRTBwNEWg{txO NXOyOpdKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PFg4PDRpPkG3OVg5PzR2PD;hQi=>
PANC1 NEfaXnBHfW6ldHnvckBCe3OjeR?= M4jMSFEh|ryP MkPZNlQhcA>? Ml;nTY5kemWjc3WgbY4heDJzV1HGNU9EUVBzIHflcoUh\XiycnXzd4lwdg>? M3jybFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUi4O|Q1Lz5zN{W4PFc1PDxxYU6=
PANC1 NYr5[VFFTnWwY4Tpc44hSXO|YYm= MnzCNUDPxE1? M1W0bFI1KGh? NIXqcVRKdmO{ZXHz[UBqdiCyMkfrbZAyKGenbnWg[ZhxemW|c3nvci=> NYLvVHZTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PFg4PDRpPkG3OVg5PzR2PD;hQi=>
PT45 NY\LRXlxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoKyTWM2OD12IN88US=> NWD1fFdjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PFg4PDRpPkG3OVg5PzR2PD;hQi=>
ACHN M1;5WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGXLZ|AzOSCq M3XD[WdKPTB;MD62OUDPxE1? NVTJUJg6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2OlI3ODZpPkG3OlYzPjB4PD;hQi=>
PC3 NITtfJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVGyNUBp NIK5cZlIUTVyPUCuO|Eh|ryP NGnDPFc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[2NlYxPid-MUe2OlI3ODZ:L3G+
A431 M3XyUmN6fG:2b4jpZ{BCe3OjeR?= NFXPOYI4OiCq NXrDblROUUN3ME20MlIh|ryP M1O4dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkmxO|Y{Lz5zN{[5NVc3OzxxYU6=
Cal27 MlHLR5l1d3SxeHnjJGF{e2G7 M2DYflczKGh? NF\kd5RKSzVyPUOuNkDPxE1? NEDpSpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[5NVc3Oyd-MUe2PVE4PjN:L3G+
CCRF-CEM NX\uXG9PS3m2b4TvfIlkKEG|c3H5 MV63NkBp MmqxTWM2OD1yLkig{txO M37q[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkmxO|Y{Lz5zN{[5NVc3OzxxYU6=
EOL1 Mo\oR5l1d3SxeHnjJGF{e2G7 NX33W2VTPzJiaB?= NHLtc2xKSzVyPUGg{txO NWDnbFRRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2PVE4PjNpPkG3OlkyPzZ|PD;hQi=>
Hec-1-A M3X4d2N6fG:2b4jpZ{BCe3OjeR?= NV:1emdyPzJiaB?= MnXNTWM2OD1zIN88US=> M4XDd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NkmxO|Y{Lz5zN{[5NVc3OzxxYU6=
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Primary bronchial epithelial cell M2XBd2tqdmG|ZTDBd5NigQ>? M2fhPFEh|ryP MmXGPVYhcA>? MljvSG1UVw>? M4nJdmlvcGmkaYTpc44hd2ZiSFTBR|ch[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gd4hwenRiY3nyZ5VqfCCldYLy[Y51ew>? NU\GU5dERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OlY4QDlpPkG5PVY3Pzh7PD;hQi=>
BxPC3 NHnM[GlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXjxUIZyPzJiaB?= M2m1OWlEPTB;Mj63JO69VQ>? Ml\zQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO3O|goRjJyMUSzO|c5RC:jPh?=
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NCI-H2122 NHTGUIxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoiwO|IhcA>? MnjrTWM2OD15LkWg{txO MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{e3PEc,OjBzNEO3O|g9N2F-
NCI-H358 MmrZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGLxeFE4OiCq MnnqTWM2OD1{LkWg{txO MoT6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO3O|goRjJyMUSzO|c5RC:jPh?=
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SKHEP1 M{L0dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MU[3NkBp M3XmUmlEPTB;Mz60OkDPxE1? NXX1ZoRKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM4PzhpPkKwNVQ{Pzd6PD;hQi=>
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MUF MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX:xNFAh|ryP MnHNOFghcA>? NX6wUpF7TE2VTx?= MVHHdo94fGhiaX7obYJqfGmxbjDheEAxNjB3IITvJFExOCC3TR?= MlPsQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
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Caco-2 MlPhT4lv[XOnIFHzd4F6 M1jodVAvOzF4IH7NJJRwKDNzLk[g{txO MYW0JIg> MkHySG1UVw>? M1vXbmV5KH[rdn:gbY5pcWKrdHnvckBw\iCqdX3hckBJTEGFMzD3bZRpKEmFNUCgc4YhOC5zNEeg{txO MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
Caco-2 MVTLbY5ie2ViQYPzZZk> NIfNOmI3NjN{IH7NJJRwKDZ|MjFOwG0> NInGTYQ1KGh? NY\BeG9xTE2VTx?= M1HBdGV5KH[rdn:gbY5pcWKrdHnvckBw\iCqdX3hckBJTEGFNTD3bZRpKEmFNUCgc4YhOTJwNjFOwG0> M1;ETFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzPFQxLz5{MEG0N|g1ODxxYU6=
Caco-2 NVjBOWpjU2mwYYPlJGF{e2G7 MonJOk4{OiCwTTD0c{A3OzJizszN NU\5SJE5PCCq M2rSbmROW09? NHnVPFdGgCC4aY\vJIlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{ege4l1cCCLQ{WwJI9nKDRzIN88US=> NU[0N3JkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
Caco-2 NFfvfGhMcW6jc3WgRZN{[Xl? MmX0NE4{OTZibl2geI8hOzFwNjFOwG0> M1i3bFQhcA>? MV\EUXNQ MUHFfEB3cX[xIHnubIljcXSrb36gc4YhcHWvYX6gTGRCSzZ? MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
HepG2 MmfST4lv[XOnIFHzd4F6 M{HGdFUh|ryP NH7EPYE3cA>? M{DjfmROW09? NUHSZYVyUW6mdXP0bY9vKG:oIFjERWMudWWmaXH0[YQhcGm|dH;u[UBi[2W2eXzheIlwdg>? NVP1[2dyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
HepG2 NVHaSIdlU2mwYYPlJGF{e2G7 NYD0ZmhlPSEQvF2= MYO2bC=> NGDvd2ZFVVOR MknnTY5lfWO2aX;uJI9nKEiGQVOtcYVlcWG2ZXSgbIl{fG:wZTDofZBmemGlZYT5cIF1cW:w NXHvO3JoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
HepG2 MlnPT4lv[XOnIFHzd4F6 M3\NfVUh|ryP MkfROog> MWDEUXNQ MYHJcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDhcJBp[S22dXL1cIlvKGGlZYT5cIF1cW:w NGTVSZI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N|g1OCd-MkCxOFM5PDB:L3G+
HepG2 NF3xO2RMcW6jc3WgRZN{[Xl? M{Plb|Uh|ryP MX:2bC=> NEX0d5pFVVOR M1L4XGlv\HWldHnvckBw\iCKRFHDMY1m\GmjdHXkJGg{N0h2IHjpd5RwdmViYXPleJlt[XSrb36= MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
COLO205 M4XkW2tqdmG|ZTDBd5NigQ>? M3TnVVExKM7:TR?= NH:4TJIzPCCq NV7iNoxiTE2VTx?= M4DRO2lvcGmkaYTpc44hd2ZiSFTBR|Eh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIhqe3SxbnWgTFMh[WOndInsZZRqd25id3n0bEBKSzVyIH;mJFIvOiEQvF2= NVHqS2I1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0OVE{PzhpPkKwOFUyOzd6PD;hQi=>
SCC4 MWLLbY5ie2ViQYPzZZk> NG[4cGQyKM7:TR?= M4HHclI1cA>? Mnf5TY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIIT1ZpVtcW5iYXPleJlt[XSrb36= NFjteW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES1NlIzPSd-MkC0OVIzOjV:L3G+
SCC4 M1f5VWtqdmG|ZTDBd5NigQ>? M{ewU|Eh|ryP NF7JfG0zPGh? Moe3TY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViYXPleJlt[XSrb36= NISwbWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES1NlIzPSd-MkC0OVIzOjV:L3G+
SCC4 MVLGeY5kfGmxbjDBd5NigQ>? M4\GSVEh|ryP NGnR[Yk2KG2rbh?= NVHmfohrSWexbnnzeEBi[3Srdnn0fUBifCCYRGKgZZN{\XO|ZXSgZZMhcW6mdXP0bY9vKG:oIFPZVFI1KGenbnWg[ZhxemW|c3nvci=> M33GVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEWyNlI2Lz5{MES1NlIzPTxxYU6=
NB4 NXLmN2RvTnWwY4Tpc44hSXO|YYm= NHrwVXA2KM7:TR?= NH7CdYQzPCCq MV;EUXNQ NHmwVWJE\WyuIHP5Z4xmKGG{cnXzeEBie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDhZ4N2dXWuYYTpc44h[XRicILlMWcyKHCqYYPl MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR7MUS0NEc,OjB2OUG0OFA9N2F-
NB4 NELGVmdMcW6jc3WgRZN{[Xl? M33XWFUh|ryP NULJdpNPOjRiaB?= MUXEUXNQ MmXjTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHHj[ZR6dGG2ZTD0eYJ2dGmwIHzleoVt NGD0V|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES5NVQ1OCd-MkC0PVE1PDB:L3G+
NB4 NGX2WHJHfW6ldHnvckBCe3OjeR?= NILhc2k2KM7:TR?= MVWyOEBp MV3EUXNQ MlLWTY5lfWO2aX;uJI9nKHB{MWfBSlEwS0mSMTDlfJBz\XO|aX;u NIm5U3k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES5NVQ1OCd-MkC0PVE1PDB:L3G+
NB4 NYnYeXJuU2mwYYPlJGF{e2G7 NFjLSHg2KM7:TR?= M{jsN|I1KGh? NVrsfZVTTE2VTx?= NFrzfmZKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIN{Bi[2W2eXzheIlwdg>? MkTrQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2OUG0OFAoRjJyNEmxOFQxRC:jPh?=
NUGC3 NI\aTJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGjnOIZIUTVyPUKuPVQh|ryP NFTFXpA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi1NFk4OSd-MkC4OVA6PzF:L3G+
A549 NGDXeIRHfW6ldHnvckBCe3OjeR?= MmrnOUDPxE1? NYT2VVJvOyCq MYnEUXNQ NVvmbGU4UW6mdXP0bY9vKG:oIHH1eI9xcGGpeTDpckBpfW2jbjDBOVQ6KGOnbHzzJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIFzDN2lK NFv3bZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi1OVIxQCd-MkC4OVUzODh:L3G+
HUT78 NWDoSo1xT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXy3NkBp Mli4SG1UVw>? M4W4SGdKPTB;MD6zJO69VQ>? MkjTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6OESyNFgoRjJyOEi0NlA5RC:jPh?=
U937 Mor6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1HyeFczKGh? NEfxSnpFVVOR NEHTPJBIUTVyPUCuN|kh|ryP NGXNPW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi4OFIxQCd-MkC4PFQzODh:L3G+
A431 MkXRR5l1d3SxeHnjJGF{e2G7 MU[3NkBp NEHRfG1EgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPDNzIHPlcIx{KG:4ZYLlfJBz\XO|aX7nJGVITlJid3n0bEBKSzVyIH;mJFQvOiEQvF2= MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
A549 NVLiSoVtS3m2b4TvfIlkKEG|c3H5 NELMeVA4OiCq NUHM[IgzS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTV2OTDj[YxteyCxdnXy[ZhxemW|c3nu[{BJTEGFIIfpeIghUUN3MDDv[kAyNjhizszN MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
CAL27 NHqySFNEgXSxdH;4bYMhSXO|YYm= M2nnPFczKGh? NIPKWppEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBESUx{NzDj[YxteyCxdnXy[ZhxemW|c3nu[{BGT0[UIIfpeIghUUN3MDDv[kA{NjJizszN M2nRN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEiwOlI6Lz5{MUC4NFYzQTxxYU6=
HeLa MoP2R5l1d3SxeHnjJGF{e2G7 M3rXeFczKGh? MVfDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJI93\XKneIDy[ZN{cW6pIFjERWMhf2m2aDDJR|UxKG:oIEGuOUDPxE1? NIXMfFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFYzQSd-MkGwPFA3Ojl:L3G+
Sf21 MXTLbY5ie2ViQYPzZZk> M4PBb|MhcA>? MnzKTY5pcWKrdHnvckBw\iCIbHHnJJRi\2enZDDoeY1idiC{ZXPvcYJqdmGwdDDISGFEQCCneIDy[ZN{\WRid3n0bEBKSzVyIH;mJFEvOiEQvF2= NEPjTlE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFYzQSd-MkGwPFA3Ojl:L3G+
SKBR3 M2\ucWN6fG:2b4jpZ{BCe3OjeR?= MniyO|IhcA>? M2nRbGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJHNMSlJ|IHPlcIx{KG:4ZYLlfJBz\XO|aX7nJGhGWjJid3n0bEBKSzVyIH;mJFIvPiEQvF2= M{D2[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEiwOlI6Lz5{MUC4NFYzQTxxYU6=
SKOV3 MWHDfZRwfG:6aXOgRZN{[Xl? NIrmXpM4OiCq Mm\UR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2tQXjNiY3XscJMhd3[ncnX4dJJme3OrbnegTGVTOiC5aYToJGlEPTBib3[gNk4zKM7:TR?= NYDvdI5vRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3OjlpPkKxNFgxPjJ7PD;hQi=>
COR-L23 NWPNcItTT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfUT|F2OTBizszN NX;UbGU3PzJiaB?= NYPlb3g5TE2VTx?= MoP0S2k2OD1zLkGg{txO NYDHOnd6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3PDdpPkKxNFgxPjR5PD;hQi=>
DU145 MlzES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlTMNVAh|ryP MWm3NkBp MoHQSG1UVw>? NEOxZ2NIUTVyPUGuNFUh|ryP NUHZNZU4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3PDdpPkKxNFgxPjR5PD;hQi=>
LoVo NVLXZYxNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MlOxNVAh|ryP MYi3NkBp MWDEUXNQ MUDHTVUxRTFwNzFOwG0> MmS5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2OFcoRjJzMEiwOlQ4RC:jPh?=
MCR5-SV2 Ml[yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWCxNEDPxE1? NWq5c|BOPzJiaB?= Mn\ESG1UVw>? MmnKS2k2OD1{LkGg{txO NIHFN3o9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFY1Pyd-MkGwPFA3PDd:L3G+
HCT116 NFPm[W1McW6jc3WgRZN{[Xl? MkDtN|Ah|ryP NVLE[HVrOzBiaB?= MlG5SG1UVw>? NVXrdohNUW6qaXLpeIlwdiCxZjDISGFEPiCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4Yh[WyyaHGteJVjfWyrbjDhZ4V1gWyjdHnvci=> NUHGcmFQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3PDdpPkKxNFgxPjR5PD;hQi=>
HCT116 NFf1UYZMcW6jc3WgRZN{[Xl? M{C4N|MxKM7:TR?= NYG3b2R3OzBiaB?= Moq2SG1UVw>? NGrseI9KdmirYnn0bY9vKG:oIFjERWMyKGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBpcXO2b37lJGg{UzliYXPleJlt[XSrb36= MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[0O{c,OjFyOEC2OFc9N2F-
U937 NEDrNXlMcW6jc3WgRZN{[Xl? NFv4Smg2KM7:TR?= M4XSSlI1KGh? NGnEfHNFVVOR NI\R[ZdKdmirYnn0bY9vKG:oIHjpd5RwdmViYXPleJltfHKjboPm[ZJie2ViYYPz[ZN{\WRiYYOgbY5kemWjc3Wgc4Yh[WOndInsZZRqd25ib3[gbIl{fG:wZTDIN{BifCCNOR?= MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7MkS5Nkc,OjF{OUK0PVI9N2F-
U937 NVjoZXNkU2mwYYPlJGF{e2G7 NH7VdIE2KM7:TR?= NX\RXXZvOjRiaB?= NWfWUXg1TE2VTx?= MlzXTY5pcWKrdHnvckBw\iCqaYP0c45mKGGlZYT5cJRz[W6|ZnXyZZNmKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIH;mJIFk\XS7bHH0bY9vKG:oIHjpd5RwdmViSEOgZZQhUzF6 M17vd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmyOFkzLz5{MUK5NlQ6OjxxYU6=
U937 M2nHOmtqdmG|ZTDBd5NigQ>? M1qwcFUh|ryP M3W5PFI1KGh? NX\1d4hGTE2VTx?= MoHlTY5pcWKrdHnvckBw\iCqaYP0c45mKGGlZYT5cJRz[W6|ZnXyZZNmKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIH;mJJBidi2jY3X0fYxifGWmIHjpd5RwdmViSESgcIV3\Wx? MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7MkS5Nkc,OjF{OUK0PVI9N2F-
U937 MkfIT4lv[XOnIFHzd4F6 MofHOUDPxE1? NYWxfXd1OTVibXnu MlrhSG1UVw>? NHXYOVBKdmirYnn0bY9vKG:oIHjpd5RwdmViYXPleJltfHKjboPm[ZJie2ViYYPz[ZN{\WRiYYOgbY5kemWjc3Wgc4YhcGm|dH;u[UBJOyCjY3X0fYxifGmxbjDs[ZZmdA>? NVzuSVc2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGyPVI1QTJpPkKxNlkzPDl{PD;hQi=>
A2780 MoHhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NID6ZZU6PiCq MnTSSG1UVw>? MlzsTWM2OD1zLk[yJO69VQ>? M2fwSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzNkO0OFMxLz5{MU[zOFQ{ODxxYU6=
COLO205 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUi5OkBp MYrEUXNQ MmDXTWM2OD1{LkGyJO69VQ>? NH7Nd3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MU[zOFQ{OCd-MkG2N|Q1OzB:L3G+
Hep3B NIDlZVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUf2PGJ[PDhiaB?= MYrEUXNQ NVnzW2pPT0l3ME2xMlIyKM7:TR?= MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMkG0Okc,OjF5MUKxOFY9N2F-
GM11272 NX\nSVFoTnWwY4Tpc44hSXO|YYm= MVmxNFAhdk1? MY[3NkBp NXqzUm1uTE2VTx?= NXHXbI1mWmWjY4TpeoF1cW:wIH;mJG1mS3B{IH31eIFvfCCneIDy[ZN{cW:wIHH0JFExKHSxIEGwNEBvVQ>? MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh2MEexOkc,OjF6NEC3NVY9N2F-
CFBE41o M2PCNWtqdmG|ZTDBd5NigQ>? NWDHRmpxPSEQvF2= NFH0OYdFVVOR MknRSI94diC{ZXf1cIF1cW:wIH;mJGhFSUN5IHH0JFAvOiC2bzC1JJVO NFHqO4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUm4OFk2QCd-MkG5PFQ6PTh:L3G+
CFBE41o NXzzXnlWTnWwY4Tpc44hSXO|YYm= NWfrXI1IOSEQvF2= NIfsTFQzPCCq MYnEUXNQ MUjS[ZN1d3KjdHnvckBw\iCFRmTSJIRmdHSjRkWwPEBufXSjboSgeJJi\m[rY3vpcochfG9iY3XscEB{fXKoYXPlJIF{KGeueXPv[o9zdXN? MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTl6NEm1PEc,OjF7OES5OVg9N2F-
DU145 NHjxcmVMcW6jc3WgRZN{[Xl? MUGyMlUh|ryP NILaU4UzPCCq M1LZU2ROW09? MUPJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhfXC{ZXf1cIF1cW:wIH;mJJAzOXejZkGgdJJwfGWrbjDlfJBz\XO|aX;u M{DydFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[wNVY3Lz5{MkK2NFE3PjxxYU6=
DU145 NGCxc|ZMcW6jc3WgRZN{[Xl? NU\YUYt1PSEQvF2= NUmzNmRqOjRiaB?= Mn\3SG1UVw>? M1XhPGlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCqaYP0c45mKEh2IHHj[ZR6dGG2aX;u M2DMN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[wNVY3Lz5{MkK2NFE3PjxxYU6=
DU145 MlOwT4lv[XOnIFHzd4F6 MWi1JO69VQ>? NUnzeWlFOjRiaB?= NHjNPGFFVVOR NI[xWnFKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaHnzeI9v\SCKMzDofZBmemGlZYT5cIF1cW:w NIDKUo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkK2NFE3Pid-MkKyOlAyPjZ:L3G+
786-0 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmnZOk4zKM7:TR?= M3;jVlczKGh? MlL6SG1UVw>? NEHH[Y9IUTVyPUOuNkDPxE1? MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
A498 M4Hhd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnX2Ok4zKM7:TR?= MljzO|IhcA>? MXnEUXNQ M2\GOGdKPTB;MT62JO69VQ>? MlL2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
A549/ATCC MlvXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1jSXFYvOiEQvF2= MmfZO|IhcA>? MlHYSG1UVw>? MnnWS2k2OD1zLk[g{txO NXTVSI4zRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
ACHN NXzJSHFmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\IVFYvOiEQvF2= MlHhO|IhcA>? NXviSHhsTE2VTx?= NVn3dXp5T0l3ME2xMlMh|ryP NXfNelYxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
BT549 MkDXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVvFdppqPi5{IN88US=> NH;qT4s4OiCq M4r5ZWROW09? NWXuUJNXT0l3ME2xMlMh|ryP M176d|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
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HOP92 NGrmNVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;UUWNLPi5{IN88US=> MkP6O|IhcA>? MYfEUXNQ NGi1OZRIUTVyPUSg{txO M1joNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
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MALME-3M MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{DRV|YvOiEQvF2= NYX1dYYzPzJiaB?= M3\TNWROW09? NEP1XIRIUTVyPUCuN|Ih|ryP M{\uSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
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MDA-MB-435 M1LoVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYW2MlIh|ryP MofvO|IhcA>? NGLxTJpFVVOR M3PHTWdKPTB;MD61JO69VQ>? Mm\DQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
MDA-MB-468 M1PQd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHYVno3NjJizszN NEXKRlM4OiCq MV;EUXNQ NUnMVXZ7T0l3ME2wMlY{KM7:TR?= NFjDeXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
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NCI-H23 MmC0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPzRnZvPi5{IN88US=> Mn\2O|IhcA>? M3vKTmROW09? NYj6eoNIT0l3ME2xJO69VQ>? M1zCU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
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NCI-H460 NIrQTnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHrVfnI3NjJizszN M1rCN|czKGh? NVHHcWJvTE2VTx?= M3iyOWdKPTB;MD63PUDPxE1? M{DVbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
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OVCAR3 NGm4U|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILDUnU3NjJizszN NWjZV21FPzJiaB?= MWDEUXNQ MoCzS2k2OD1zLkOg{txO NY\jcWRpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
OVCAR4 NWD6flQ5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmDaOk4zKM7:TR?= MYK3NkBp NXKx[|BLTE2VTx?= MXTHTVUxRTRizszN MmftQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
OVCAR5 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPLeWpnPi5{IN88US=> MWm3NkBp MnTrSG1UVw>? M3jYOWdKPTB;MD63PUDPxE1? M1TWS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
OVCAR8 NWjDXYdET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml3sOk4zKM7:TR?= NF\wRoY4OiCq NWDtfVJUTE2VTx?= MmXpS2k2OD1yLkWg{txO MoTyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
RXF393 NUDndoFoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnntOk4zKM7:TR?= MU[3NkBp NX3IOlFCTE2VTx?= MYHHTVUxRTFwMzFOwG0> MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
SF268 NHzYNGFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEK1Uno3NjJizszN MWG3NkBp M{T6Z2ROW09? M{PxTmdKPTB;MT62JO69VQ>? MoS1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
SF295 MnLoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXLZNIM2Pi5{IN88US=> M{\MW|czKGh? MlzoSG1UVw>? NHfkTVVIUTVyPUGuOkDPxE1? MlPJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
SF539 NYK0UlBmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfvOGQ3NjJizszN MWm3NkBp Mk\KSG1UVw>? NEfNOnlIUTVyPUKg{txO M3zQVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
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SNB19 NH7E[4tIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1PXb|YvOiEQvF2= MnzLO|IhcA>? MW\EUXNQ MkTpS2k2OD1yLk[zJO69VQ>? NInrWJE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
SNB75 NHjY[opIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU\qeIpmPi5{IN88US=> M2G5cVczKGh? MUDEUXNQ M3T5TGdKPTB;MD63PUDPxE1? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
SR MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\MeJJ4Pi5{IN88US=> NULvNJpJPzJiaB?= NH;TeINFVVOR NGHHdpJIUTVyPUCuOEDPxE1? M4HnXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
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TK10 MkHQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4nlNlYvOiEQvF2= MnL4O|IhcA>? NV3aUlJyTE2VTx?= NVfzcJRTT0l3ME2wMlY{KM7:TR?= NFfsSWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
UACC257 MnO3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFn5eIc3NjJizszN MoX1O|IhcA>? NUPNWmlwTE2VTx?= Mk\yS2k2OD1yLkWg{txO M2fCc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
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Bel7404 NXSyW|JbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXnuPIVPPzJiaB?= NUPDXWVLTE2VTx?= MljZS2k6OD1zODFOwG0> MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MUO3Okc,OjNyNkGzO|Y9N2F-
BGC823 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVi5dJlUPzJiaB?= MljMSG1UVw>? M2DZO2dKQTB;Nz61JO69VQ>? Mo[3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkGzO|YoRjJ|ME[xN|c3RC:jPh?=
MGC803 M2PETWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUS3NkBp MlfGSG1UVw>? MknLS2k6OD1zMD64JO69VQ>? MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MUO3Okc,OjNyNkGzO|Y9N2F-
SGC7901 NYrEemN5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUK3NkBp Mn3lSG1UVw>? NHL2e5RIUTlyPUmuOEDPxE1? M4Cz[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|ME[xN|c3Lz5{M{C2NVM4PjxxYU6=
SKHEP1 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVi3NkBp M4rFdGROW09? MXvHTVkxRTF5IN88US=> NXPofnQ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlE{PzZpPkKzNFYyOzd4PD;hQi=>
SMMC7721 NGP2R2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWT6SnpuPzJiaB?= MV7EUXNQ M3P2S2dKQTB;MkCg{txO M3TqflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|ME[xN|c3Lz5{M{C2NVM4PjxxYU6=
NUGC3 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\ENVAh|ryP MWHHTV0zNjl2IN88US=> NFvuTo89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{G2N|M{Oid-MkOxOlM{OzJ:L3G+
A2780 CisR NVLJXHA4U2mwYYPlJGF{e2G7 Ml7xNVghcA>? NFLI[m5KdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuOFQh|ryP NFvSU2U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
CAL27 sens M3vPd2tqdmG|ZTDBd5NigQ>? NGGxfWgyQCCq NYjuWJlJUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlM3KM7:TR?= MkmxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
CAL27 CisR NHf2fGhMcW6jc3WgRZN{[Xl? NXnmUnhXOThiaB?= NIq5cmdKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuOlEh|ryP MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
KYSE-510 CisR M2LEfGtqdmG|ZTDBd5NigQ>? M1\sNFE5KGh? NGrnSY5KdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuOVkh|ryP NH\OTHY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
KYSE-510 sens NYTHZ4xyU2mwYYPlJGF{e2G7 NFH0XI0yQCCq NUSwV2pQUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlch|ryP MoXLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
MDA-MB-231 sens MUTLbY5ie2ViQYPzZZk> M{O4c|E5KGh? NFHhO25KdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR|UxKG:oIECuOlEh|ryP MlyyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
MDA-MB-231 CisR NGWyVVZMcW6jc3WgRZN{[Xl? NUT1R2k4OThiaB?= MYjJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNkGg{txO MmW1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
ES2 Ml\6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUW0PEBp MmDkSG1UVw>? NHXoeHBKSzVyPUGyMlch|ryP MnLmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUO0OFkoRjJ|NEmzOFQ6RC:jPh?=
MDA-MB-231 M2TkbWtqdmG|ZTDBd5NigQ>? MWSxNEDPxE1? M4j6fFMxKG2rbh?= MUPEUXNQ MYXJcohq[mm2aX;uJI9nKEiGQVOyZS=> MkLQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUO0OFkoRjJ|NEmzOFQ6RC:jPh?=
MDA-MB-231 M3WxZmtqdmG|ZTDBd5NigQ>? NGHicWgyKM7:TR?= NH[zTVgzPCCq NWfSTodnTE2VTx?= MV3Jcohq[mm2aX;uJI9nKEiGQVO2JIF{e2W|c3XkJIF{KGGlZYT5cIF1cW:wIHzleoVteyCxZjD0eYJ2dGmw MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7M{S0PUc,OjN2OUO0OFk9N2F-
HS68 M4XnTmN6fG:2b4jpZ{BCe3OjeR?= NFXteZkyOCEQvF2= MoKwO|IhcA>? MX;EUXNQ M3TEdWlEPTB;ND62JO69VQ>? MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzV5MEW0Nkc,OjN3N{C1OFI9N2F-
MEF NVL3R2ZFS3m2b4TvfIlkKEG|c3H5 NEOxTnoyOCEQvF2= NVj1NpVPPzJiaB?= MXnEUXNQ NGrOVmlKSzVyPUSuOEDPxE1? NVvI[ZZWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1O|A2PDJpPkKzOVcxPTR{PD;hQi=>
BGC823 Ml\kS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnX4SG1UVw>? MUTHTVUxRTNwMEmg{txO MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZyMUewOkc,OjN4MEG3NFY9N2F-
High5 MorKT4lv[XOnIFHzd4F6 MUSyOEBp MlTTTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDOkB4cXSqIFnDOVAhd2ZiMD6wPUDPxE1? NUf0[olnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NFI2OjNpPkKzOlAzPTJ|PD;hQi=>
High5 MXLLbY5ie2ViQYPzZZk> M2rDSlI1KGh? NEXMTHBKdmirYnn0bY9vKG:oIHj1cYFvKHKnY3;tZolv[W62IFjERWMyKHerdHigTWM2OCCxZjCwMlEzKM7:TR?= M1nGW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkCyOVI{Lz5{M{[wNlUzOzxxYU6=
High5 MlXXT4lv[XOnIFHzd4F6 NHO2VYIzPCCq MXnJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVOzJJdqfGhiSVO1NEBw\iByLkG3JO69VQ>? M3vzWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkCyOVI{Lz5{M{[wNlUzOzxxYU6=
A549 NX\3RWJRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX:2elBjTE2VTx?= NV7GZXV4UUN3ME2xMlA4QSEQvF2= NW\ZZmRIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2OFQzOTBpPkKzOlQ1OjFyPD;hQi=>
HCT116 NH;2OWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1XDeGROW09? MkGxTWM2OD1yLke1O{DPxE1? MnzNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NESyNVAoRjJ|NkS0NlExRC:jPh?=
K562 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH7INItFVVOR M2ezT2lEPTB;MD62OFUh|ryP NHj0[mo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[0OFIyOCd-MkO2OFQzOTB:L3G+
GBC-SD MoTzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYi1JO69VQ>? MYS3NkBp NFPNOXBFVVOR NHu0fo9KSzVyPUG0Mlgh|ryP M{XGcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C3NlYxLz5{M{ewO|I3ODxxYU6=
HGC27 NIXVUYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4n6dFUh|ryP M2\yTFczKGh? MoLHSG1UVw>? NHvWPFVKSzVyPUWuOEDPxE1? NWfrbZY2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFczPjBpPkKzO|A4OjZyPD;hQi=>
HL60 NUHJfm5pU2mwYYPlJGF{e2G7 NFrLVW42KM7:TR?= M4LoOFI1KGh? NH;VSHZFVVOR M3\ZWGlvcGmkaYTpc44hd2ZiSFTBRy=> Mn;wQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5MEeyOlAoRjJ|N{C3NlYxRC:jPh?=
Vero MkPjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEHqXHI4OiCq MWfEUXNQ NFPkWlNKSzVyPUCuPUDPxE1? NXq5VIEyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3PFY1PTJpPkKzO|g3PDV{PD;hQi=>
NCI60 NUn6VZY3S3m2b4TvfIlkKEG|c3H5 MkTMOFghcA>? MlPRSG1UVw>? M2LDNGdKRTBwNUOg{txO MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDBzNUOyO{c,OjRyMUWzNlc9N2F-
SW620 NV\L[YJ7S3m2b4TvfIlkKEG|c3H5 NV76fG41PDhiaB?= NYXEfoY5TE2VTx?= MVTHTV0xNjV2IN88US=> NIXsVZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECxOVMzPyd-MkSwNVU{Ojd:L3G+
U251 NGTiNXVEgXSxdH;4bYMhSXO|YYm= MVW0PEBp MYnEUXNQ M2fkXGdKRTFwNUOg{txO MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDBzNUOyO{c,OjRyMUWzNlc9N2F-
KM12 NXfjRZVRS3m2b4TvfIlkKEG|c3H5 NIDNdmc1QCCq M4CxRmROW09? MkO3S2k:OS56ODFOwG0> M1XjclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEG1N|I4Lz5{NECxOVMzPzxxYU6=
Hs578T NIC1b5FEgXSxdH;4bYMhSXO|YYm= MnqxOFghcA>? MmXoSG1UVw>? NWjKeYRFT0l;ND64N{DPxE1? M{H2S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEG1N|I4Lz5{NECxOVMzPzxxYU6=
MDA-MB-231 M1;wW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVO0PEBp NY[2SXM4UUN3ME2wMlE3KM7:TR?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7NUCxOkc,OjRyOUWwNVY9N2F-
MCF7 M3rIOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3P2S|Q5KGh? M2LYUGdKRTBwNEWg{txO M3TsU|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUG5OVU2Lz5{NEGxPVU2PTxxYU6=
MDA-MB-435 M3XCU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXnMeGtYPDhiaB?= Ml7BS2k:OC56ODFOwG0> NEPiXWU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEGxPVU2PSd-MkSxNVk2PTV:L3G+
AsPC1 NFrBcVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2LlN|MxKM7:TR?= MmXEOFghcA>? MnTESG1UVw>? MXHJR|UxRTNwNk[g{txO MkDQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzOEWzO|goRjJ2MUi1N|c5RC:jPh?=
Jurkat E6.1 MVXLbY5ie2ViQYPzZZk> M4nJR|IhcA>? MYfEUXNQ MUTJcohq[mm2aX;uJI9nKEiGQVO0JJdqfGhiSVO1NEBw\iByLkC0JO69VQ>? NFezc5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEK2NVg3Oid-MkSyOlE5PjJ:L3G+
KG1 NFTR[ZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1jGPFQ5KGh? MnL6TWM2OD1zLkG1JO69VQ>? NITKb|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEWyOVAxOyd-MkS1NlUxODN:L3G+
HEL MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX3LSYNpPDhiaB?= M{HFfGROW09? MXXJR|UxRTBwNEmg{txO MlK2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4OUSwOVUoRjJ2Nkm0NFU2RC:jPh?=
HSC3 MnjGR5l1d3SxeHnjJGF{e2G7 MXGxNkBp MoTMSG1UVw>? M1XzN2lEPTB;MD63NUDPxE1? MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4NkW2NEc,OjR5Nk[1OlA9N2F-
HT-29 M1PGPWN6fG:2b4jpZ{BCe3OjeR?= NH35U4gyOiCq MmfvSG1UVw>? MXzJR|UxRTBwN{Kg{txO MnzkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5Nk[1OlAoRjJ2N{[2OVYxRC:jPh?=
KB MVfDfZRwfG:6aXOgRZN{[Xl? NYHCZmRXOTJiaB?= NV;mbJFqTE2VTx?= MYDJR|UxRTBwN{Wg{txO MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4NkW2NEc,OjR5Nk[1OlA9N2F-
H460 NU\lN|B4S3m2b4TvfIlkKEG|c3H5 MkPkNVIhcA>? NV[2V21kTE2VTx?= NFHBXFhKSzVyPUCuPFIh|ryP MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4NkW2NEc,OjR5Nk[1OlA9N2F-
MONE1 NVXvd4M4S3m2b4TvfIlkKEG|c3H5 MkjuNVIhcA>? MWXEUXNQ M1PabWlEPTB;MT6xPEDPxE1? MkDpQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5Nk[1OlAoRjJ2N{[2OVYxRC:jPh?=
MKN45 NX\lOm9TS3m2b4TvfIlkKEG|c3H5 MnHNNVIhcA>? M3SzbGROW09? MWrJR|UxRTFwMkWg{txO MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4NkW2NEc,OjR5Nk[1OlA9N2F-
TSGH NGi5d|lEgXSxdH;4bYMhSXO|YYm= M4jBV|EzKGh? MlPVSG1UVw>? MYTJR|UxRTJwMEmg{txO MkLNQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5Nk[1OlAoRjJ2N{[2OVYxRC:jPh?=
HL60 MonUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmrJO|IhcA>? NWLTTFV{TE2VTx?= NIHkcpBIUTVyPUCuPVMh|ryP NXTnOFdXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA2ODBpPkK0PVAxPTByPD;hQi=>
HEK293 NEnuXmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYO3NkBp MWHEUXNQ M4GwdGdKPTB;MUOg{txO MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDlyMEWwNEc,OjR7MEC1NFA9N2F-
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UACC-893 MkTlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnHTTWM2OD1{Mz6xNFM5KM7:TR?= NUnnTpMzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
NCI-H2347 NUjneWNwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{HzUmlEPTB;MkOuNlY4PCEQvF2= Mm\oQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
U-118-MG MmHLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFT3VI9KSzVyPUKzMlczOTdizszN NHzjZ3c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NCI-H596 NIK2[XZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NX;tfpMxUUN3ME2yN{46ODV3IN88US=> MoWyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
MZ1-PC MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn;yTWM2OD1{ND62O|U6KM7:TR?= M4PUTFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
SW1417 M{Hye2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Moj4TWM2OD1{NT6yOFk5KM7:TR?= NF;ZTZo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NCI-H82 MkjuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkfyTWM2OD1{Nj60Nlg5KM7:TR?= M1HjelxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
COLO-678 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEDkeJhKSzVyPUK3MlQ6OTlizszN MnjKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
KURAMOCHI M1PjTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFPud|dKSzVyPUK4MlEzPiEQvF2= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
NCI-H1650 NGjlfHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NILseYVKSzVyPUOwMlE6OjRizszN MmrHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
HCC1954 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2jRfWlEPTB;M{GuN|Y3PiEQvF2= M4fJXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
GAK MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3eyNmlEPTB;M{KuNlYyPiEQvF2= MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
YAPC NULkRZd6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFGyOJZKSzVyPUOzMlc3PDhizszN M3\HclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H2228 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYPJR|UxRTN2LkO2NFUh|ryP M3f6W|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
VMRC-RCZ M3X1dmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2TYTmlEPTB;M{[uN|AzOiEQvF2= NHvZe|k9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NMC-G1 MlK1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlXFTWM2OD1|Nz6wOVI6KM7:TR?= NInhXpo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
D-336MG NGi0RWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLLOZNoUUN3ME20NU45PDR{IN88US=> Moj2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
UACC-62 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DBT2lEPTB;NEKuOVUxPSEQvF2= Mki0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
C-33-A Mn\RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTR3LkOyOVYh|ryP M2XuR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
HCC1937 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\PboZ7UUN3ME20O{4yQTV6IN88US=> NFjXOoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
HDLM-2 NYKxVWgzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTR5LkKyPFch|ryP M4DNOVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H727 NFjkbJJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXm3eWk5UUN3ME20O{4zPDVzIN88US=> MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
NY NG\3TnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2nkbGlEPTB;NEmuN|kxQCEQvF2= MlrwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NCI-H2291 NYL5ZodDT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn[2TWM2OD12OT64N|AyKM7:TR?= MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Ac-Histone H4 / Ac-Histone H3; 

PubMed: 21598070     


D54 and U87 glioma cells were treated with lower concentrations (1-3 µM) of vorinostat. The effects on histone acetylation was assessed by immunoblotting. 

phospho-CDK1; 

PubMed: 21598070     


Changes in level of inhibitory phosphorylation of cdk1 after treatment with vorinostat was determined at 24 and 48 h (using a Tyr-15 residue phospho-specific antibody) along with total cdk1 as loading control. 

phospho-Cdc25c / Cdc25c; 

PubMed: 21598070     


Time course of changes in phospho-cdc25c levels after exposure to vorinostat (5 µM).

Cyclin B1; 

PubMed: 21598070     


Cyclin B1 levels were determined in D54 and U87 cells after treatment with various doses of vorinostat at 24 and 48 h.

c-Myc / c-Raf / Akt / Cyclin D1 / CDK4; 

PubMed: 19440035     


Cell lysates from JeKo-1 and Mo2058 cells exposed to vorinostat  were immunoblotted for c-Myc, c-Raf, AKT, cyclin D1, CDK4 and acetylated histone H3. The levels of β-actin served as the loading control.

p-eIF2α / ATF4 / CHOP ; 

PubMed: 20072656     


Western blot was performed to evaluate activated eIF2α (P-eIF2α), translational up-regulation of ATF4, and CHOP expression in U251 cells after vorinostat exposure.

p21; 

PubMed: 21598070     


To determine the dose response relationship to vorinostat, cells were exposed to various higher concentrations (5–10 µM) of the agent and the levels of p21 determined by immunoblotting.

21598070 19440035 20072656
Immunofluorescence
HDAC1 / CK19; 

PubMed: 29917299     


Immunocytochemistry to detect the expression of HDAC1 and CK 19 in PDAC cells with/without Vorinostat treatment. HDAC1 and CK19 were stained red and green, respectively. The Hoechst‐stained nuclei appear blue. Scale bar: 100 μm. The CK19 expression in PSN1 and MiaPaCa2 was increased by Vorinostat treatment.

Nrf2; 

PubMed: 20566897     


Vorinostat induced translocation of Nrf2 from cytosol to nucleus. U937 cells were treated with 2μM vorinostat for 18 hours and then cytospun. Cells were fixed and labeled for Nrf2 protein (red) and nucleus (blue, 4,6-diamidino-2-phenylindole) as described in “Immunofluorescence and confocal microscopy.” Images were taken by Nikon Eclipse TE2000 confocal microscope with 40×/1.30 numeric aperture oil objective lens. Ctrl indicates control cells without treatment; and Vor, vorinostat.

α-SMA; 

PubMed: 26330748     


Representative immunofluorescence images and the quantification graph showing the effect of vorinostat (2.5 µM), TGIF1, and TGIF2 siRNA knockdown on αSMA. Control human corneal fibroblast cultures (-TGFβ) showed no αSMA staining. Cultures treated with TGFβ1 (5 ng/ml) showed a robust αSMA staining. Vorinostat treatment markedly attenuated TGFβ-evoked αSMA staining. The knockdown of TGIF1 (+TGFβ+vorinostat+TGIF1siRNA) and TGIF2 alone (+TGFβ+vorinostat+TGIF2siRNA) or in combination (+TGFβ +vorinostat +TGIF1 +2siRNA) markedly blocked the effect of vorinostat on αSMA. Scale bar=100 μm.

Ac-STAT3 / IGF2; 

PubMed: 27086926     


Immunofluorescence staining of Ac-STAT3 and IGF2 after vorinostat treatment.

29917299 20566897 26330748 27086926
Growth inhibition assay
Cell apoptosis; 

PubMed: 19440035     


Cells were treated with indicated concentrations of vorinostat for 48 hours. Following this, the percentage of annexin-V-stained apoptotic cells was determined by flow cytometry. Values represented as bar graphs are the mean of 3 experiments plus or minus the standard error (SE)

Cell viability; 

PubMed: 27086926     


The effects of vorinostat on the viability of the indicated NSCLC cell lines and their corresponding acquired VoR sublines. Cell viability was determined by the MTT assay (n = 3).

19440035 27086926
ELISA
IGF2; 

PubMed: 27086926     


Determination of vorinostat-induced IGF2 production by ELISA (n = 3). The conditioned mediums (CMs) obtained from cells treated with vorinostat (5 μM) for 2 days were used for ELISA. 

IL-13 / IL-10 / IL-5; 

PubMed: 18541724     


Effect of vorinostat on TH1/TH2 cytokine secretion in HL cell lines. Cells were incubated with medium or vorinostat (5 μM) for 24 hours and supernatants were examined for cytokine levels by ELISA. Each value represents a mean of 3 independent experiments done in triplicate (± SEM).

M-CSF; 

PubMed: 23042302     


Primary PyMT tumor cells were treated with SAHA (0-1000nM) for varying time points (8–48h), and supernatants were assayed by ELISA for M-CSF  secretion. *P < 0.05 and **P < 0.001 versus control treatment. 

MMP-9; 

PubMed: 23042302     


Primary PyMT tumor cells were treated with SAHA (0-1000nM) for varying time points (8–48h), and supernatants were assayed by ELISA for MMP-9 secretion. *P < 0.05 and **P < 0.001 versus control treatment. 

27086926 18541724 23042302
In vivo Administration of Vorinostat (~100 mg/kg/day) significantly inhibits the growth of CWR22 human prostate xenografts in nude mice with tumor reductions of 78%, 97% and 97%, at doses of 25 mg/kg/day, 50 mg/kg/day and 100 mg/kg/day, respectively, compared with control. Vorinostat induces the accumulation of acetylated core histones and prostate-specific antigen mRNA expression in CWR22 cells, resulting in higher levels of serum prostate-specific antigen than predicted from tumor volume alone. [2] Oral administration of Vorinostat (0.67g/L) crosses the blood-brain barrier, increases histone acetylation in the brain, and dramatically improves the motor impairment in the R6/2 mice model of Huntington's disease. [5]

Protocol

Kinase Assay:

[1]

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Immunoprecipitation-HDAC assays:

The lysate of Jurkat cells is incubated for 1 hour on ice and cleared by centrifugation at 12,000 g for 10 minutes at 4 °C. Supernatants are precleared with 30 μL of 50% protein G-Sepharose slurry for 1 hour at 4 °C. Beads are pelleted by centrifugation and supernatants are incubated for 1 hour at 4 °C with 10 μg of IgG fraction from anti-HDAC1 or HDAC3 polyclonal antisera (preincubated 2 hours at room temperature with either the homologous or heterologous immunizing peptide). Both antisera are raised in rabbits against the carboxylterminal peptide of HDAC1 and HDAC3 by using synthetic peptides coupled to keyhole limpet hemocyanin. 30 μL of 50% protein G-Sepharose slurry is added for 1 hour at 4 °C. Immune complexes are pelleted by centrifugation and washed three times with 1 mL of lysis buffer. Beads are resuspended in 200 μL of HDAC buffer (20 mM Tris-HCl, pH 8.0/150 mM NaCl/10% glycerol), and the HDAC assay is performed with an 3H-acetylated peptide corresponding to amino acids 1-24 of histone H4. Released [3H]acetic acid is quantified by scintillation counting. For inhibitions studies, the immunoprecipitated complexes are preincubated with the different concentrations of Vorinostat for 30 minutes at 4 °C.
Cell Research:

[2]

- Collapse
  • Cell lines: LNCaP, PC-3, and TSU-Pr1
  • Concentrations: Dissolved in DMSO, final concentrations ~7.5 μM
  • Incubation Time: 1, 2, 3 and 4 days
  • Method:

    Cells are exposed to various concentrations of Vorinostat for 1, 2, 3 and 4 days. Cell viability is assessed by trypan blue dye exclusion.


    (Only for Reference)
Animal Research:

[2]

- Collapse
  • Animal Models: Male BALB/c nude (nu/nu) mice implanted with CWR22 tumor cells
  • Dosages: ~100 mg/kg/day
  • Administration: Injection i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 52 mg/mL (196.74 mM)
Water Insoluble
Ethanol '''3 mg/mL warmed
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 264.3
Formula

C14H20N2O3

CAS No. 149647-78-9
Storage powder
in solvent
Synonyms MK0683, Suberoylanilide hydroxamic acid
Smiles C1=CC=C(C=C1)NC(=O)CCCCCCC(=O)NO

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Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

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Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

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Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03022565 Withdrawn Drug: Vorinostat Uveal Melanoma University of Miami|University of Miami Sylvester Comprehensive Cancer Center January 2020 Early Phase 1
NCT03167437 Recruiting Drug: Vorinostat|Drug: Ustekinumab Crohn''s Disease National Institute of Allergy and Infectious Diseases (NIAID)|National Institutes of Health Clinical Center (CC) October 30 2017 Phase 1|Phase 2
NCT03056495 Recruiting Drug: N-hydroxy-N''-phenyl-octanediamide (Vorinostat) Alzheimer Disease German Center for Neurodegenerative Diseases (DZNE)|University Hospital Bonn|University of Göttingen September 28 2017 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

  • Question 1:

    What’s the recommendation about reconstitution of the compound for in vivo animal study?

  • Answer:

    We recommend the following vehicle for S1047: 2% DMSO, 40% PEG 300, 5% Propylene glycol, 1% Tween 80 at 5 mg/mL.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID