Vorinostat (SAHA)

Name: Vorinostat (SAHA)
Brand: Selleck Chemicals
SKU: S1047
Rating: 5 (421 reviews)

For research use only.

Catalog No.S1047 Synonyms: MK0683, Suberoylanilide hydroxamic acid

421 publications

CAS No. 149647-78-9

Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.

Selleck's Vorinostat (SAHA) has been cited by 421 publications

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Nat Biotechnol, 2020, 10.1038/s41587-019-0388-4

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Cell, 2019, 176(1-2):198-212

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Cell, 2019, 36(2):179-193

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Cancer Cell, 2019, 36(2):179-193

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Cell, 2018, 174(3):536-548.e21

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Cell Metab, 2018, 5;27(6):1294-1308.e7

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Nature, 2017, 551(7679):247-250

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Cancer Discov, 2017, 7(12):1450-1463

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Cancer Cell, 2016, 29(3):311-323

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Nat Biotechnol, 2015, 10.1038/nbt.3130

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Nat Med, 2015, 21(10):1163-71

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Nat Biotechnol, 2011, 29(3):255-65

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Neuro Oncol, 2021, 23(3):376-386

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Neuro Oncol, 2021, 23(2):226-239

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Elife, 2021, 10e63810

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Cells, 2021, 10(5)1001

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Biochem Pharmacol, 2021, 188:114546

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Int J Mol Sci, 2021, 22(9)4559

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J Enzyme Inhib Med Chem, 2021, 36(1):34-47

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J Dermatol Sci, 2021, S0923-1811(21)00005-0

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EMBO Rep, 2020, 21:e50162

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J Immunother Cancer, 2020, 8(2)e001037

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Theranostics, 2020, 1;10(3):1433-1453

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Cancer, 2020, 1;126(9):2024-2033

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Cancers (Basel), 2020, 12(11)E3172

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PLoS Pathog, 2020, 16;16(3):e1008341

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Front Cell Dev Biol, 2020, 17;8:158

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J Neuroinflammation, 2020, 22;17(1):125

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ACS Infect Dis, 2020, 29

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Mol Cancer Res, 2020, 10.1158/1541-7786.MCR-19-0669

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Front Oncol, 2020, 10:296

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J Virol, 2020, 4;94(10) pii: e01923-19

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Biochem Pharmacol, 2020, 3;178:114067

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Cancer Cell Int, 2020, 19;20:58

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HIV Med, 2020, 21(11):747-757

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Am J Transl Res, 2020, 12(10):6187-6203

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Andrology, 2020, 10.1111/andr.12922

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BMC Microbiol, 2020, 20(1):28

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PLoS One, 2020, 15;15(5):e0232985

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Hum Cell, 2020, 10.1007/s13577-020-00420-z

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J Med Invest, 2020, 67(3.4):343-350

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Sci Rep, 2020, 10(1):12864

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Oncol Lett, 2020, 19(1):460-468

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Sci Adv, 2020, 6(29):eaba1941

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Colloids Surf B Biointerfaces, 2020, 188:110753

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Acta Pharm Sin B, 2020, 10(4):615-627

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Cancer Sci, 2020, 111(7):2374-2384

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Oncogene, 2020, 10.1038/s41388-020-01394-w

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Viruses, 2020, 12(10)E1067

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Oncogene, 2020, 10.1038/s41388-020-01408-7

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Br J Cancer, 2020, 10.1038/s41416-020-01191-y

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Mol Cancer Ther, 2020, 19(2):637-649

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Genes Dev, 2020, 34(5-6):428-445

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Biomater Sci, 2020, 10.1039/d0bm00119h

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Cell Cycle, 2020, 1-16

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Life Sci, 2020, 248:117469

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Chin Med J (Engl), 2020, 5;133(11):1304-1311

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Pharmaceuticals (Basel), 2020, 13(11)E345

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Pharm Biol, 2020, 58(1):231-238

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PLoS Biol, 2020, 18(12):e3000941

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Nat Commun, 2019, 10(1):4192

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J Pineal Res, 2019, 66(3):e12556

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Haematologica, 2019, 10.3324/haematol.2018.211110

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Haematologica, 2019, 104(11):2225-2240

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Theranostics, 2019, 9(2):540-553

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Cancer Res, 2019, 79(7):1658-1670

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Cell Rep, 2019, 27(11):3331-3344

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Elife, 2019, 8

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Int J Cancer, 2019, 145(9):2496-2508

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Mol Ther, 2019, 27(5):1039-1050

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Cancers (Basel), 2019, 11(5)E645

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Cells, 2019, 8(12)

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Front Immunol, 2019, 9:3162

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Pharmacol Res, 2019, 139:524-534

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Pharmacol Res, 2019, 146:104281

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J Neurochem, 2019, 10.1111/jnc.14935

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Cell Host Microbe, 2018, 24(5):689-702

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Nat Commun, 2018, 13;9(1):2720

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Cancer Res, 2018, 78(10):2705-2720

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J Control Release, 2018, 275:117-128

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Haematologica, 2018, 103(4):679-687

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J Hematol Oncol, 2018, 11(1):32

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Cancer Lett, 2018, 432:121-131

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Acta Biomater, 2018, 71:261-270

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Sci Signal, 2018, 11(557)

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PLoS Pathog, 2018, 14(6):e1007061

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Oncoimmunology, 2018,

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Mol Cancer Ther, 2018, 17(12):2767-2779

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Cancer Sci, 2018, 109(8):2520-2531

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J Virol, 2018, 92(10)

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J Virol, 2018, 92(6)

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Retrovirology, 2018, 15(1):36

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Exp Cell Res, 2018, 371(1):231-237

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BMC Cancer, 2018, 18(1):466

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Oncol Rep, 2018, 39(1):264-270

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Biochem Biophys Res Commun, 2018, 498(3):481-486

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Cell Biochem Funct, 2018, 36(8):398-407

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J Med Virol, 2018, 90(9):1478-1485

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Curr Protoc Pharmacol, 2018, 81(1):e41

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Alcohol Clin Exp Res, 2018, 42(11):2160-2171

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Oncol Lett, 2018, 15(5):6107-6114

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JCI Insight, 2018, 3(4)95824

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JCI Insight, 2018, 3(22)125568

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Nat Struct Mol Biol, 2017, 24(1):47-54

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Clin Cancer Res, 2017, 23(12):3139-3149

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Proc Natl Acad Sci USA, 2017,

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Clin Cancer Res, 2017, 23(12):3084-3096

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Oncogene, 2017, 36(34):4859-4874

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Oncogene, 2017, 36(12):1707-1720

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Br J Pharmacol, 2017, 174(20):3608-3622

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Sci Signal, 2017, 10(508)eaan8355

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Cell Death Dis, 2017, 8(1):e2570

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Cell Death Dis, 2017,

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Mol Ther Oncolytics, 2017,

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Cell Chem Biol, 2017, 24(11):1356-1367.e8

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Cancer Discov, 2017, 7(12):1450-1463

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Eur J Med Chem, 2017, 127:531-553

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Cancer Sci, 2017, 108(7):1485-1492

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Pharm Res, 2017, 34(3):668-679

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Cancer Res Treat, 2017, 49(2):374-386

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Mol Immunol, 2017, 87:76-85

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Int J Cell Biol, 2017, 2017:6169310

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PLoS One, 2017, 12(10):e0186620

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Nucleus, 2017, 8(5):515-533

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Oncotarget, 2017, 8(11):17995-18009

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CPT Pharmacometrics Syst Pharmacol, 2017, 6(11):756-764

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Sci Rep, 2017, 7(1):2290

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JCI Insight, 2017, 2(1):e85811

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Oncotarget, 2017,

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JCI Insight, 2017, 2(6):e90196

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Bioorg Med Chem, 2017, 25(7):2105-2132

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Oncotarget, 2017,

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Cell Host Microbe, 2017, 21(5):569-579

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Oncotarget, 2017, 8(61):103797-103814

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Ann Oncol, 2016, 27(3):508-13

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Nat Commun, 2016, 7:11363

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Cancer Res, 2016, 76(23):7001-7011

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mBio, 2016, 7(6)

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Oncoimmunology, 2016,

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Cancer Lett, 2014, 351(1):50-8

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Cell Death Dis, 2014, 5:e1177

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Mol Cancer Ther, 2014, 13(12):2784-92

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J Mol Biol, 2014, 426(20):3442-53

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Hum Gene Ther, 2014, 25(7):599-608

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Cancer Cell Int, 2014, 10.1186/s12935-014-0112-x

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Cancer Cell Int, 2014, 14(1):112

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Mol Cell Biol, 2014, 34(7):1280-9

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Mol Med Rep, 2014, 10(5):2729-35

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Pharmacology Research Perspectives, 2014, 10.1002/prp2.43

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Oncotarget, 2014, 5(15):5965-77

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Blood, 2013, 14;121(11):2038-50

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Blood, 2013, 123(5):706-16

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Proc Natl Acad Sci USA, 2013, 111(3):E364-73

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Cancer Res, 2013, 73(8):2428-34

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Cell Rep, 2013, 5(6):1679-89

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Neuropsychopharmacology, 2013, 38(9):1674-84

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Oncogene, 2013, 32(10):1316-29

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Cell Death Dis, 2013, 4:e733

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Cell Death Dis, 2013, 4:e951

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Br J Haematol, 2013, 160(2):188-98

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AIDS, 2013, 27(18):2853-62

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PLoS Comput Biol, 2013, 9(2):e1002887

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Mol Carcinog, 2013, 52(3):218-28

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Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description

Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.

Targets

HDAC ^[1]
(Cell-free assay)

~10 nM

In vitro

Vorinostat inhibits the activities of HDAC1 and HDAC3 with IC50 of 10 nM and 20 nM, respectively. Vorinostat also results in a marked hyperacetylation of histone H4. ^[1] Vorinostat inhibits the growth of three prostate cancer cell lines LNCaP, PC-3 and TSU-Pr1 at micromolar concentrations (2.5-7.5 μM), and induces dose-dependent cell death in LNCaP cells. ^[2] Vorinostat treatment in MCF-7 cells inhibits cell proliferation at an IC50 of 0.75 μM resulting in the accumulation of cells in the G1 and G2-M phase of the cell cycle. Vorinostat also induces differentiation in the estrogen receptor-negative cell line SKBr-3 and the retinoblastoma-negative cell line MDA-468. ^[3] Vorinostat treatment at 1 μM for 8 hours or more is sufficient to irreversibly induce apoptosis of human multiple myeloma (MM) cells. The gene expression profiles of Vorinostat treated MM cells are not hallmarked by global transcriptional activation, but by coordinated transcriptional changes of specific functional groups of genes such as cytokine-induced proliferative/survival signaling cascades, oncogenes-tumor suppressor genes, regulators of apoptosis, DNA synthesis-repair and cell cycle, and proteasome-ubiquitin function. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
H1299	MljxT4lv[XOnIFHzd4F6		M1vtTVIxKG2rbh?=	NYL3SIFOTE2VTx?=	NXnaT21MUW6qaXLpeI9zgSCjY4Tpeol1gSCjZ3HpcpN1KEiGQVOg[Y57gW2nIIfpeIghUUN3MDDv[kAxNjF7NDFOwG0>	NH\v[Gs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEWyNVQzOid-MUS1NlE1OjJ:L3G+
HT1080	NEWzcY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NGnDe5g4OiCq	Mn\0SG1UVw>?	NWTpNVZ2UUN3ME2yMlQh\|ryP	MonLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ{N{CxO\|UoRjF{MkewNVc2RC:jPh?=
K562	M4\qdGtqdmG\|ZTDBd5NigQ>?		MXu2JIg>	M13qNGROW09?	NGW1S5JKdmirYnn0bY9vKG:oIHj1cYFvKGirc4TvcoUh\GWjY3X0fYxie2ViKH3pfJR2emVib3[gTGRCSzFiYX7kJGhFSUN{KTD3bZRpKEmFNUCgc4YhOC5yMTFOwG0>	MkTDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ2MUmzPFAoRjF{NEG5N\|gxRC:jPh?=
A20	MVzLbY5ie2ViQYPzZZk>		M{fWSFEhcA>?	MnP5SG1UVw>?	M{ToSoFvfGlvSFTBR\|Eh[WO2aY\peJkhf2m2aDDJR\|UxKG:oIECuNVEzKM7:TR?=	NGfkbHc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMUm2NFQ5QSd-MUG5OlA1QDl:L3G+
Friend leukemic cell	MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXe1NEDPxE1?	M{\KXlczKGh?	NUOydJdMTE2VTx?=	Mo[zTWM2OD1yLkm5JO69VQ>?	MnLuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJzMEm5NVMoRjF{MUC5PVE{RC:jPh?=
H1299	Mn[yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MljtO\|IhcA>?	MknWSG1UVw>?	MlTHTWM2OD15LkK0JO69VQ>?	NVK2c4wxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS1NlE1OjJpPkG0OVIyPDJ{PD;hQi=>
HeLa	NIHjSHNMcW6jc3WgRZN{[Xl?		MmPnN\|AhdWmw		NH2wU\|BKdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6\|dDDobZN1d26nIHTlZYNmfHmuYYPlJJdqfGhiSVO1NEBw\iByLkK4JO69VQ>?	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTZyM{m0PUc,OTV4MEO5OFk9N2F-
LOX-IMVI	MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MVy0PEBp		M2WzeWlEPTB;MT6zJO69VQ>?	NH\qdGs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUexOVQ4OCd-MUW3NVU1PzB:L3G+
NCI-H226	M1;WbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		MnX0OFghcA>?		NEW1NlFKSzVyPUKuOkDPxE1?	NYC4bnNQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3NVU1PzBpPkG1O\|E2PDdyPD;hQi=>
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MCF7	MUnGeY5kfGmxbjDBd5NigQ>?	NG\KfY0yOCEQvF2=	NH34Zo05KGh?	MoPvSG1UVw>?	M13UNWlv\HWldHnvckBw\iC2dXL1cIlvKGi7cHXyZYNmfHmuYYTpc44>	M{HKOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6Mk[5NlI3Lz5zOEK2PVIzPjxxYU6=
HEK293	NGPocZZMcW6jc3WgRZN{[Xl?		NHfSb5IyKGh?	NX7v[4ptTE2VTx?=	NF;YVGtKdmirYnn0bY9vKG:oIFjERWMyKHerdHigTWM2OCCxZjCwMlA{KM7:TR?=	NF6xSm89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEOwPFU3Oyd-MUizNFg2PjN:L3G+
HEK293	NF\p[XlMcW6jc3WgRZN{[Xl?		NX;BeYY{OSCq	M1z3bGROW09?	MnfRTY5pcWKrdHnvckBw\iCKRFHDN{B4cXSqIFnDOVAhd2ZiMD6wOVch\|ryP	NW\4SFY4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizNFg2PjNpPkG4N\|A5PTZ\|PD;hQi=>
G401	MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		Mn[yO\|IhcA>?	MkjOSG1UVw>?	NUXHfmNMUUN3ME2xJO69VQ>?	NVSxc2tIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizO\|A{PzNpPkG4N\|cxOzd\|PD;hQi=>
HeLa	MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NG[3RYU4OiCq	M3u5dWROW09?	M3fLOmlEPTB;MD60OkDPxE1?	MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN5MEO3N{c,OTh\|N{CzO\|M9N2F-
Human renal epithelial cell	NFjFdm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		MXG3NkBp	NVTpdW5tTE2VTx?=	Mm\BTWM2OD1zNDFOwG0>	NFrHZlg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO3NFM4Oyd-MUizO\|A{PzN:L3G+
U937	MmX6SpVv[3Srb36gRZN{[Xl?	MlryOUDPxE1?	M3j5dFI1KGh?		NIn3b2RKdmS3Y4Tpc44hd2ZiaHnzeI9v\SCKNDDofZBmemGlZYT5cIF1cW:w	Mlj5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|OEGyN\|goRjF6M{ixNlM5RC:jPh?=
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U937	MYLGeY5kfGmxbjDBd5NigQ>?	NVficJJJPSEQvF2=	Mn[2NlQhcA>?		M4TQWmlv\HWldHnvckBw\iCyMkGg[ZhxemW\|c3nvci=>	MlnyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|OEGyN\|goRjF6M{ixNlM5RC:jPh?=
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U937	MVTGeY5kfGmxbjDBd5NigQ>?	MWC1JO69VQ>?	M{f4RlI1KGh?		MWXJcoR2[3Srb36gc4Yh\3KjboXsc4N6fGmlIHTp[oZmemWwdHnheIlwdg>?	Mmi1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|OEGyN\|goRjF6M{ixNlM5RC:jPh?=
U937	NHzNbHpMcW6jc3WgRZN{[Xl?	MkTrOUDPxE1?	MoPtNlQhcA>?		NHPTTVdKdmirYnn0bY9vKG:oIHj1cYFvKEiGQVOx	NXfBNnhKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizPFEzOzhpPkG4N\|gyOjN6PD;hQi=>
U937	MUjLbY5ie2ViQYPzZZk>	NYi5Uo1VPSEQvF2=	NWrBUXB{OjRiaB?=		NF\DeFFKdmirYnn0bY9vKG:oIHj1cYFvKEiGQVO0	NEfSTW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEO4NVI{QCd-MUizPFEzOzh:L3G+
HEK-TE	NXz3cXYxTnWwY4Tpc44hSXO\|YYm=	MXW1JO69VQ>?	MmnxOog>	MUHEUXNQ	NEDXOG9KdmO{ZXHz[UBqdiCqaYP0c45mKEh\|IHHj[ZR6dGG2aX;u	NYjnXFFjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0N\|QyPDRpPkG4OFM1OTR2PD;hQi=>
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Human microvascular epithelial cell	NET0SI5EgXSxdH;4bYMhSXO\|YYm=	NFvWPIY2OCEQvF2=	Ml72O\|IhcA>?	M3vyNWROW09?	MWDJR\|UxRTFwMzFOwG0>	NEXmOow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOES5OFQ3Oyd-MUi0PVQ1PjN:L3G+
Hup T3	MXnDfZRwfG:6aXOgRZN{[Xl?	MUO1NEDPxE1?	NEftS4k4Omh?	NGf0VllFVVOR	MWTJR\|UxRTBwODFOwG0>	NFPCS5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOES5OFQ3Oyd-MUi0PVQ1PjN:L3G+
Mia Paca2	MWrDfZRwfG:6aXOgRZN{[Xl?	Mor0OVAh\|ryP	NYr1fW5uPzKq	NFvpe\|hFVVOR	NELKWIJKSzVyPUGuNUDPxE1?	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR7NES2N{c,OTh2OUS0OlM9N2F-
Panc 04.03	NFzYbGFEgXSxdH;4bYMhSXO\|YYm=	MXS1NEDPxE1?	NX7hNG02PzKq	NFLTcopFVVOR	MoTZTWM2OD1zLkKg{txO	M2\NN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEm0OFY{Lz5zOES5OFQ3OzxxYU6=
SU 86.86	MUnDfZRwfG:6aXOgRZN{[Xl?	Mn7jOVAh\|ryP	NETI[II4Omh?	MnraSG1UVw>?	MnTTTWM2OD1zLkOg{txO	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR7NES2N{c,OTh2OUS0OlM9N2F-
HEK293	NIm2fodMcW6jc3WgRZN{[Xl?		M1[1WFMhcA>?		Mm\3TY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDISGFEPiCneIDy[ZN{cW:wIIfpeIghUUN3MDDv[kAxNjBzOTFOwG0>	MlKyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh3NUi2OlkoRjF6NUW4OlY6RC:jPh?=
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RAW264.7	MYDGeY5kfGmxbjDBd5NigQ>?	MkXzNkDPxE1?	NUT0[lNMOy53IHi=	MnXLSG1UVw>?	MVzBcpRqdWGuYYLpZYwh[WO2aY\peJkh[WejaX7zeEBRdGG\|bX;kbZVuKG[jbHPpdIFzfW1iaX7m[YN1\WRid3n0bEBKSzVyIH;mJFAvOTFizszN	M{fkNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NkS0PVY6Lz5zOE[0OFk3QTxxYU6=
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HEK293	MkezSpVv[3Srb36gRZN{[Xl?	MWKxNEDPxE1?	MXqyOEBp	M2D3N2ROW09?	M2DyWGFkfGm4YYTpc44hd2ZibX;1d4UhX262M3Ggd4lodmGuaX7nJIV5eHKnc4Pl[C=>	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB{Mk[3OUc,OTlyMkK2O\|U9N2F-
HCT116	NXvieWtkT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		M3qzZlczcA>?	M2LnN2ROW09?	MWfJR\|UxRTBwM{Gg{txO	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB6NEK5OEc,OTlyOESyPVQ9N2F-
HeLa	NYDsXIhFU2mwYYPlJGF{e2G7	NYrjOFR[OiEQvF2=	Mkj5N\|AhdWmw	NFfCOYVFVVOR	M3;LeWlvcGmkaYTpc44hd2ZiSFTBR\|IhcW5iaIXtZY4hUGWOYTDj[YxteyC5aYToJGlEPTBib3[gNE4yKM7:TR?=	NWjvPZpxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwPFQzQTRpPkG5NFg1Ojl2PD;hQi=>
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T24	MYXLbY5ie2ViQYPzZZk>		MWOzJIg>		NYH0SlRQUW6mdXP0bY9vKG:oIHHsdIhiKHS3YoXsbY4h[WOndInsZZRqd25id3n0bEBGSzVyIH;mJFAvOjVizszN	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB6NEO5OUc,OTlyOESzPVU9N2F-
HeLa	NX7DVll[U2mwYYPlJGF{e2G7		NVizWFNlOTVibXnu	MWDEUXNQ	MnHSTY5pcWKrdHnvckBw\iCKRFHDOkBnem:vIH71Z4xm[XJiZYj0doFkfCC5aYToJGlEPTBib3[gNE4xQDV3IN88US=>	M4nRNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEmzPFg1Lz5zOUC5N\|g5PDxxYU6=
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SKMES1	NEHacXNEgXSxdH;4bYMhSXO\|YYm=		M1zLXFczKGh?	MonoSG1UVw>?	M4Tic2lEPTB;Mj60NkDPxE1?	MnfJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyOUO4PFQoRjF7MEmzPFg1RC:jPh?=
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SHSY5Y	M4ruVmtqdmG\|ZTDBd5NigQ>?	NHWyVZcyOCEQvF2=	NEK2Nng1QCCq	M4TGOmROW09?	M{nkdGlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD61JO69VQ>?	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN2NEG3OUc,OTl\|NESxO\|U9N2F-
SHSY5Y	NEnXbY1EgXSxdH;4bYMhSXO\|YYm=	NHrUW4UyOCEQvF2=	Mo\yOFghcA>?	MmXxSG1UVw>?	NFf5[GxKSzVyPUKg{txO	MmLvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl\|NESxO\|UoRjF7M{S0NVc2RC:jPh?=
DMS114	Ml71S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		NWX4UFJSPDhiaB?=		M1PCdWdKPTB;Mj65JO69VQ>?	Ml\3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2MUmyNFUoRjF7NEG5NlA2RC:jPh?=
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SNB78	MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MYi0PEBp		MXnHTVUxRTF4IN88US=>	MlrFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl2MUmyNFUoRjF7NEG5NlA2RC:jPh?=
HeLa	MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NFrwcJc4OiCq	NX7pfnJjTE2VTx?=	MV7JR\|UxRTBwNE[g{txO	NES0OGg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUS0NVg1Pid-MUm0OFE5PDZ:L3G+
BHK	M1XrOmtqdmG\|ZTDBd5NigQ>?	M4r2[VUh\|ryP	NGPXXXozPCCq	MUTEUXNQ	NG[1SmNKdmirYnn0bY9vKG:oIFjERWM4KGW6cILld5Nm\CCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBxdGG\|bXGgcYVu[nKjbnWgSoRmdHSjNUC4JGNHXFJicILveIVqdg>?	MY[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl4Nke4PUc,OTl7Nk[3PFk9N2F-
CFBE41o-	NI\3SZZMcW6jc3WgRZN{[Xl?	NUXaNFNPPSEQvF2=	MYK4JIg>	MU\EUXNQ	M4PiOGlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCqeYDldoFk\XS7bHH0bY9vKG:oIHjpd5RwdmViSEO=	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl4Nke4PUc,OTl7Nk[3PFk9N2F-
CFBE41o-	M4exW2tqdmG\|ZTDBd5NigQ>?	MlezOUDPxE1?	NV;POJllPCCq	MXvEUXNQ	MV\Jcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5ibYX0ZY51KE[mZXz0ZVUxQCCFRmTSJJBzd3SnaX6gcIV3\Wx?	NV36bo5iRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OlY4QDlpPkG5PVY3Pzh7PD;hQi=>
CFBE41o-	M1jCUGtqdmG\|ZTDBd5NigQ>?	NUPr[49yOSEQvF2=	M1f4U\|EhcA>?	M3\T[2ROW09?	MmTsTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIFPGWHIheHKxdHXpckBt\X[nbB?=	MkHQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Nk[3PFkoRjF7OU[2O\|g6RC:jPh?=
Primary bronchial epithelial cell	M4jNeWtqdmG\|ZTDBd5NigQ>?	NYjPPIlCOSEQvF2=	M3;yWFI1KGh?	MmezSG1UVw>?	NWPEeGZ7UW6qaXLpeIlwdiCxZjDISGFEPyCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4YhdXW2YX70JGZl\Wy2YUWwPEBETlSUIIDyc5RmcW5iYYDpZ4FtKHO3cn\hZ4UhdG:lYXzpfoF1cW:w	NUHXXlhIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OlY4QDlpPkG5PVY3Pzh7PD;hQi=>
CFBE41o-	NWHU[nFNU2mwYYPlJGF{e2G7	NWjHTlFMOSEQvF2=	M1X5VVUh\GG7cx?=	M2TJNGROW09?	M{HXfWlvcGmkaYTpc44hd2ZiSFTBR\|ch[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gd5Ri[mmuaYrheIlwdiCxZjDG[IVtfGF3MEigR2ZVWiCycn;0[Ylv	Ml7rQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Nk[3PFkoRjF7OU[2O\|g6RC:jPh?=
CFBE41o-	MXfLbY5ie2ViQYPzZZk>	NX\0SnBSOSEQvF2=	MoGyNlQhcA>?	MkK3SG1UVw>?	M4\BcGlvcGmkaYTpc44hd2ZiSFTBR\|ch[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gSoRmdHSjNUC4JGNHXFJicILveIVqdiC2cnHm[olkc2mwZx?=	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl4Nke4PUc,OTl7Nk[3PFk9N2F-
CFBE41o-	NInCXXVMcW6jc3WgRZN{[Xl?	NXHxXFREPSEQvF2=	NV[1[5hxOjRiaB?=	MYTEUXNQ	NXPr[VZCUW6qaXLpeIlwdiCxZjDISGFEPyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBz[XSnIH;mJI1ifHW{YYTpc44hd2ZiQ1\UVkBxem:2ZXnuJJRwKGm2czDncJlkd2[xcn2=	NXjqUYFERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OlY4QDlpPkG5PVY3Pzh7PD;hQi=>
CFBE41o-	MWfLbY5ie2ViQYPzZZk>	Mln2OUDPxE1?	MU[yOEBp	MUDEUXNQ	MUTJcohq[mm2aX;uJI9nKEiGQVO3JIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIIP0ZYJqdGm2eTDv[kBH\GWudHG1NFghS0[WUjDwdo91\Wmw	M{GzWlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OU[2O\|g6Lz5zOUm2Olc5QTxxYU6=
CFBE41o-	NEDZeXhMcW6jc3WgRZN{[Xl?	MVm1JO69VQ>?	MnW2NlQhcA>?	NVHl[VVDTE2VTx?=	MnLWTY5pcWKrdHnvckBw\iCKRFHDO{Bie3Onc4Pl[EBieyClb4Ly[YN1cW:wIH;mJI12fGGwdDDG[IVtfGF3MEigR2ZVWiC2cnHm[olkc2mwZzD0c{Bk\WyuIIP1doZi[2ViYYOg[4x6[2:ob4Lt	M1fJcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OU[2O\|g6Lz5zOUm2Olc5QTxxYU6=
Primary bronchial epithelial cell	M3rNNGtqdmG\|ZTDBd5NigQ>?	NV;mOYp7PSEQvF2=	M3jaNFI1KGh?	M2PpPWROW09?	NHPI[WNKdmirYnn0bY9vKG:oIFjERWMh[XO\|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJI12fGGwdDDG[IVtfGF3MEigR2ZVWiCycn;0[YlvKGGyaXPhcEB{fXKoYXPlJIxw[2GuaYrheIlwdg>?	Mni3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Nk[3PFkoRjF7OU[2O\|g6RC:jPh?=
Primary bronchial epithelial cell	NY\YRnNpU2mwYYPlJGF{e2G7	NXXNdIZYOSEQvF2=	M3Had\|k3KGh?	MoXmSG1UVw>?	NUPqOGdOUW6qaXLpeIlwdiCxZjDISGFEPyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckB{cG:{dDDjbZJkfWm2IHP1dpJmdnS\|	NGLXb5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm2Olc5QSd-MUm5OlY4QDl:L3G+
BxPC3	NWrke4NWT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		MmPaO\|IhcA>?		NXficI1uUUN3ME2yMlch\|ryP	MnjCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO3O\|goRjJyMUSzO\|c5RC:jPh?=
CAL27	M2\TT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NVy5S4Y{PzJiaB?=		NIPhflJKSzVyPUOuO\|Ih\|ryP	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{e3PEc,OjBzNEO3O\|g9N2F-
Capan1	MkLSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		NFzlSYE4OiCq		NXmxRnJ2UUN3ME23MlMh\|ryP	MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{e3PEc,OjBzNEO3O\|g9N2F-
HCC827	NEK1W3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NHnJ[Xk4OiCq		NF\JcIpKSzVyPUGuPEDPxE1?	MnrTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO3O\|goRjJyMUSzO\|c5RC:jPh?=
LNCAP	M4niZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		MnfzO\|IhcA>?		NVW2O49VUUN3ME2wMlU5KM7:TR?=	MnjIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO3O\|goRjJyMUSzO\|c5RC:jPh?=
NCI-H2122	M1;3[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NEXFO\|Q4OiCq		NX3qR2tpUUN3ME23MlUh\|ryP	NXjVe4FzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM4PzhpPkKwNVQ{Pzd6PD;hQi=>
NCI-H358	MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NV64cnA4PzJiaB?=		NHLIUmJKSzVyPUKuOUDPxE1?	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{e3PEc,OjBzNEO3O\|g9N2F-
SCC15	M1iyR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NUn6NWVSPzJiaB?=		NWrvNnNWUUN3ME2yMlQ6KM7:TR?=	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{e3PEc,OjBzNEO3O\|g9N2F-
SCC25	Ml7mS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MVq3NkBp		MWHJR\|UxRTFwOUOg{txO	NHGzUoY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N\|c4QCd-MkCxOFM4Pzh:L3G+
SKHEP1	MkLkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MmP1O\|IhcA>?		M2fncGlEPTB;Mz60OkDPxE1?	NVTxOHhRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM4PzhpPkKwNVQ{Pzd6PD;hQi=>
BJ	M1;ON2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MmKyNVAxKM7:TR?=	M2DmVlQ5KGh?	Ml7USG1UVw>?	M{f5Nmdzd3e2aDDpcohq[mm2aX;uJIF1KDBwMEWgeI8hOTByIIXN	NUDGTolURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
MUF	M4fOfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MXixNFAh\|ryP	MkO0OFghcA>?	M3n6OmROW09?	NGLrO25Iem:5dHigbY5pcWKrdHnvckBifCByLkC1JJRwKDFyMDD1US=>	MlrWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
Caco-2	NI\VPJBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MXixNFAh\|ryP	NIPBWG01QGh?	NV3JdXJSTE2VTx?=	NXLw[pZ6UUN3ME24MlEh\|ryP	NX[yXnBRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
Caco-2	NGLQPHpMcW6jc3WgRZN{[Xl?	M{jUN\|Uh\|ryP	NYPlPZZPPCCq	NWC3[2hRTE2VTx?=	MUTJcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDobZN1d26nIHHj[ZR6dGG2aX;uJGlEPTBib3[gNE4xOzdizszN	Mlf0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
Caco-2	NEnKfWtMcW6jc3WgRZN{[Xl?	MV[wMlMyPiCwTTD0c{A{OS54IN88US=>	MlXlOEBp	MmPsSG1UVw>?	M2foV2V5KH[rdn:gbY5pcWKrdHnvckBw\iCqdX3hckBJTEGFMTD3bZRpKEmFNUCgc4YhOC5zMUmg{txO	MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
Caco-2	M2\zcmtqdmG\|ZTDBd5NigQ>?	NYnyfYY3OC5\|MU[gcm0hfG9iM{GuOkDPxE1?	MmDSOEBp	Ml35SG1UVw>?	Mlv3SZghfmm4bzDpcohq[mm2aX;uJI9nKGi3bXHuJGhFSUN\|IIfpeIghUUN3MDDv[kAxNjF2NzFOwG0>	MoDCQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
Caco-2	MkC5T4lv[XOnIFHzd4F6	NXXPT2h2Pi5\|MjDuUUB1dyB4M{Kg{txO	M4m1S\|QhcA>?	Ml\QSG1UVw>?	M{\SS2V5KH[rdn:gbY5pcWKrdHnvckBw\iCqdX3hckBJTEGFNTD3bZRpKEmFNUCgc4YhOTJwNjFOwG0>	M1ixNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzPFQxLz5{MEG0N\|g1ODxxYU6=
Caco-2	NFzGSGdMcW6jc3WgRZN{[Xl?	MWK2MlMzKG6PIITvJFY{OiEQvF2=	NFvXc3A1KGh?	MnjESG1UVw>?	MmTxSZghfmm4bzDpcohq[mm2aX;uJI9nKGi3bXHuJGhFSUN5IIfpeIghUUN3MDDv[kA1OSEQvF2=	NEnzZ\|M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N\|g1OCd-MkCxOFM5PDB:L3G+
Caco-2	NXLlemJPU2mwYYPlJGF{e2G7	MofINE4{OTZibl2geI8hOzFwNjFOwG0>	M3PVSFQhcA>?	NYXCb4lMTE2VTx?=	MYTFfEB3cX[xIHnubIljcXSrb36gc4YhcHWvYX6gTGRCSzZ?	MnXXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
HepG2	NYnvfmM4U2mwYYPlJGF{e2G7	M2nyOlUh\|ryP	Ml3XOog>	NFHUclJFVVOR	MUHJcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDobZN1d26nIHHj[ZR6dGG2aX;u	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
HepG2	NF3hdHlMcW6jc3WgRZN{[Xl?	M{GxS\|Uh\|ryP	MlfEOog>	NUXTd3dZTE2VTx?=	M2LXUWlv\HWldHnvckBw\iCKRFHDMY1m\GmjdHXkJIhqe3SxbnWgbJlx\XKjY3X0fYxifGmxbh?=	NUX5eWcyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
HepG2	NY[1d5JHU2mwYYPlJGF{e2G7	NYHJPXVkPSEQvF2=	MU[2bC=>	NFnTPFFFVVOR	NGriT4xKdmS3Y4Tpc44hd2ZiSFTBR{1u\WSrYYTl[EBidHCqYT30eYJ2dGmwIHHj[ZR6dGG2aX;u	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
HepG2	MV7LbY5ie2ViQYPzZZk>	NGnsfYs2KM7:TR?=	MV[2bC=>	M3;IPWROW09?	MkfUTY5lfWO2aX;uJI9nKEiGQVOtcYVlcWG2ZXSgTFMwUDRiaHnzeI9v\SCjY3X0fYxifGmxbh?=	M1L2fFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzPFQxLz5{MEG0N\|g1ODxxYU6=
COLO205	M1KwR2tqdmG\|ZTDBd5NigQ>?	NVTMNlZTOTBizszN	NEHnb5AzPCCq	MXrEUXNQ	MUDJcohq[mm2aX;uJI9nKEiGQVOxJIF{e2W\|c3XkJIF{KGmwZIXjeIlwdiCxZjDobZN1d26nIFizJIFk\XS7bHH0bY9vKHerdHigTWM2OCCxZjCyMlIh\|ryP	MlznQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NUGzO\|goRjJyNEWxN\|c5RC:jPh?=
SCC4	MmD3T4lv[XOnIFHzd4F6	NV7OepBGOSEQvF2=	MX6yOIg>		NXTBUFVnUW6qaXLpeIlwdiCxZjDISGFEKGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJJR2[nWuaX6gZYNmfHmuYYTpc44>	M3\2UlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEWyNlI2Lz5{MES1NlIzPTxxYU6=
SCC4	MnnFT4lv[XOnIFHzd4F6	MmXGNUDPxE1?	NV3ETnJoOjSq		M37sXGlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDobZN1d26nIHHj[ZR6dGG2aX;u	M1LjW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEWyNlI2Lz5{MES1NlIzPTxxYU6=
SCC4	M3XsN2Z2dmO2aX;uJGF{e2G7	NVLB[mQ3OSEQvF2=	MoXpOUBucW5?		M{DoR2Fod26rc4SgZYN1cX[rdImgZZQhXkSUIHHzd4V{e2WmIHHzJIlv\HWldHnvckBw\iCFWWCyOEBo\W6nIHX4dJJme3Orb36=	NVTmZ3I6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0OVIzOjVpPkKwOFUzOjJ3PD;hQi=>
NB4	NVrrfGpyTnWwY4Tpc44hSXO\|YYm=	MYO1JO69VQ>?	MnjYNlQhcA>?	NGj3bXBFVVOR	MkPSR4VtdCCleXPs[UBienKnc4SgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iYXPjeY12dGG2aX;uJIF1KHC{ZT3HNUBxcGG\|ZR?=	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR7MUS0NEc,OjB2OUG0OFA9N2F-
NB4	MUnLbY5ie2ViQYPzZZk>	MUi1JO69VQ>?	MXyyOEBp	M1vwOmROW09?	NITQPYZKdmirYnn0bY9vKG:oIFjERWMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gZYNmfHmuYYTlJJR2[nWuaX6gcIV3\Wx?	NI[5U2w9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES5NVQ1OCd-MkC0PVE1PDB:L3G+
NB4	MkXBSpVv[3Srb36gRZN{[Xl?	M1vEcVUh\|ryP	NGDlWlkzPCCq	NXvEboZ2TE2VTx?=	NFfNbZNKdmS3Y4Tpc44hd2ZicEKxW2FHOS:FSWCxJIV5eHKnc4Ppc44>	MnS2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2OUG0OFAoRjJyNEmxOFQxRC:jPh?=
NB4	M4LPT2tqdmG\|ZTDBd5NigQ>?	MVy1JO69VQ>?	NHToVIMzPCCq	M2[2cWROW09?	M3;B[mlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDobZN1d26nIFizJIFk\XS7bHH0bY9v	MmD4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2OUG0OFAoRjJyNEmxOFQxRC:jPh?=
NUGC3	NVrweWhyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MW\HTVUxRTJwOUSg{txO	M17PXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOEWwPVcyLz5{MEi1NFk4OTxxYU6=
A549	NUnDSoJZTnWwY4Tpc44hSXO\|YYm=	M1e3dVUh\|ryP	Ml;lN{Bp	Mkn6SG1UVw>?	M1LCcGlv\HWldHnvckBw\iCjdYTvdIhi\3liaX6gbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCOQ{PJTS=>	M4WwU\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOEW1NlA5Lz5{MEi1OVIxQDxxYU6=
HUT78	MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		M2K1VVczKGh?	MlzSSG1UVw>?	MlLBS2k2OD1yLkOg{txO	NGi1b289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi4OFIxQCd-MkC4PFQzODh:L3G+
U937	NVTP[IlOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		MXO3NkBp	M2\1fWROW09?	NUHyV\|RbT0l3ME2wMlM6KM7:TR?=	M{nDVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyOEi0NlA5Lz5{MEi4OFIxQDxxYU6=
A431	MXTDfZRwfG:6aXOgRZN{[Xl?		Ml7zO\|IhcA>?		NVvMSmxmS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hSTR\|MTDj[YxteyCxdnXy[ZhxemW\|c3nu[{BGT0[UIIfpeIghUUN3MDDv[kA1NjJizszN	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
A549	MkXSR5l1d3SxeHnjJGF{e2G7		MoTiO\|IhcA>?		MkfER5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{BwfmW{ZYjwdoV{e2mwZzDISGFEKHerdHigTWM2OCCxZjCxMlgh\|ryP	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
CAL27	Mki1R5l1d3SxeHnjJGF{e2G7		MkfCO\|IhcA>?		M3\2VWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNCVDJ5IHPlcIx{KG:4ZYLlfJBz\XO\|aX7nJGVITlJid3n0bEBKSzVyIH;mJFMvOiEQvF2=	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
HeLa	MWrDfZRwfG:6aXOgRZN{[Xl?		NVXLfXluPzJiaB?=		MXvDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDI[WxiKGOnbHzzJI93\XKneIDy[ZN{cW6pIFjERWMhf2m2aDDJR\|UxKG:oIEGuOUDPxE1?	Mn\UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2NlkoRjJzMEiwOlI6RC:jPh?=
Sf21	MX\LbY5ie2ViQYPzZZk>		M1T1NVMhcA>?		NIKyR\|VKdmirYnn0bY9vKG:oIF\sZYchfGGpZ3XkJIh2dWGwIILlZ49u[mmwYX70JGhFSUN6IHX4dJJme3OnZDD3bZRpKEmFNUCgc4YhOS5{IN88US=>	M136blxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEiwOlI6Lz5{MUC4NFYzQTxxYU6=
SKBR3	NIDFTlJEgXSxdH;4bYMhSXO\|YYm=		NWnO[o1pPzJiaB?=		NUPiSHJNS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0uEUkOgZ4VtdHNib4\ldoV5eHKnc4PpcochUEWUMjD3bZRpKEmFNUCgc4YhOi54IN88US=>	NGXxW5I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFYzQSd-MkGwPFA3Ojl:L3G+
SKOV3	MomxR5l1d3SxeHnjJGF{e2G7		MYS3NkBp		MYDDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT29XOyClZXzsd{BwfmW{ZYjwdoV{e2mwZzDISXIzKHerdHigTWM2OCCxZjCyMlIh\|ryP	Mor6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2NlkoRjJzMEiwOlI6RC:jPh?=
COR-L23	Mlu4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MYOxNEDPxE1?	NVvhOWptPzJiaB?=	NYTrXWJvTE2VTx?=	NYDzXZQ{T0l3ME2xMlEh\|ryP	MUe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[0O{c,OjFyOEC2OFc9N2F-
DU145	M{DlVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	Mn;JNVAh\|ryP	M3[3V\|czKGh?	MYnEUXNQ	NHnl[lRIUTVyPUGuNFUh\|ryP	MoPFQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2OFcoRjJzMEiwOlQ4RC:jPh?=
LoVo	NXvwfWNlT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M1zLdVExKM7:TR?=	MnXYO\|IhcA>?	NELhOYFFVVOR	MWXHTVUxRTFwNzFOwG0>	M{Swe\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEiwOlQ4Lz5{MUC4NFY1PzxxYU6=
MCR5-SV2	NF7XT29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MlraNVAh\|ryP	MnnDO\|IhcA>?	NHzCUG5FVVOR	MYrHTVUxRTJwMTFOwG0>	NEXxWmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFY1Pyd-MkGwPFA3PDd:L3G+
HCT116	NUjvcI1bU2mwYYPlJGF{e2G7	MorIN\|Ah\|ryP	MVSzNEBp	Mn3oSG1UVw>?	M2m5dGlvcGmkaYTpc44hd2ZiSFTBR\|Yh[XO\|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIFteGijLYT1ZpVtcW5iYXPleJlt[XSrb36=	NY\zUHZkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3PDdpPkKxNFgxPjR5PD;hQi=>
HCT116	MVrLbY5ie2ViQYPzZZk>	NFfQXmg{OCEQvF2=	MX6zNEBp	NYr5OoNRTE2VTx?=	MVrJcohq[mm2aX;uJI9nKEiGQVOxJIF{e2W\|c3XkJIF{KGmwZIXjeIlwdiCxZjDobZN1d26nIFizT\|kh[WOndInsZZRqd25?	NWOzNXY2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3PDdpPkKxNFgxPjR5PD;hQi=>
U937	NXHJSFRMU2mwYYPlJGF{e2G7	M2m2U\|Uh\|ryP	M1fyVFI1KGh?	NWnUPHZPTE2VTx?=	MmfnTY5pcWKrdHnvckBw\iCqaYP0c45mKGGlZYT5cJRz[W6\|ZnXyZZNmKGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIH;mJIFk\XS7bHH0bY9vKG:oIHjpd5RwdmViSEOgZZQhUzl?	NFzNfFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5NlQ6Oid-MkGyPVI1QTJ:L3G+
U937	NGDnWm1McW6jc3WgRZN{[Xl?	Ml35OUDPxE1?	Mln4NlQhcA>?	NEDyUo1FVVOR	MWrJcohq[mm2aX;uJI9nKGirc4TvcoUh[WOndInseJJidnOoZYLhd4Uh[XO\|ZYPz[YQh[XNiaX7jdoVie2Vib3[gZYNmfHmuYYTpc44hd2ZiaHnzeI9v\SCKMzDheEBMOTh?	Mn;UQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OUK0PVIoRjJzMkmyOFkzRC:jPh?=
U937	MoLyT4lv[XOnIFHzd4F6	NYL0do5rPSEQvF2=	NHLBU\|UzPCCq	MW\EUXNQ	MYnJcohq[mm2aX;uJI9nKGirc4TvcoUh[WOndInseJJidnOoZYLhd4Uh[XO\|ZYPz[YQh[XNiaX7jdoVie2Vib3[gdIFvNWGlZYT5cIF1\WRiaHnzeI9v\SCKNDDs[ZZmdA>?	NGDlPGQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5NlQ6Oid-MkGyPVI1QTJ:L3G+
U937	NE\TNGFMcW6jc3WgRZN{[Xl?	NIXqPIE2KM7:TR?=	M1TSSVE2KG2rbh?=	M4LmS2ROW09?	NV;BcIhZUW6qaXLpeIlwdiCxZjDobZN1d26nIHHj[ZR6dHS{YX7z[oVz[XOnIHHzd4V{e2WmIHHzJIlv[3KnYYPlJI9nKGirc4TvcoUhUDNiYXPleJlt[XSrb36gcIV3\Wx?	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7MkS5Nkc,OjF{OUK0PVI9N2F-
A2780	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MmC2PVYhcA>?	Mme5SG1UVw>?	NIDaTXlKSzVyPUGuOlIh\|ryP	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTZ\|NESzNEc,OjF4M{S0N\|A9N2F-
COLO205	MmHyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		M3LKRlk3KGh?	NF33Z4pFVVOR	MnT1TWM2OD1{LkGyJO69VQ>?	NGHlNJg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MU[zOFQ{OCd-MkG2N\|Q1OzB:L3G+
Hep3B	NYjvU4JOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		MXe0PEBp	Mn;wSG1UVw>?	M2DxUmdKPTB;MT6yNUDPxE1?	M2mxfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzN{GyNVQ3Lz5{MUexNlE1PjxxYU6=
GM11272	NXvkVWRoTnWwY4Tpc44hSXO\|YYm=	NGjNS2QyODBibl2=	NX7PcWpRPzJiaB?=	MUDEUXNQ	NICyXZJT\WGldHn2ZZRqd25ib3[gUYVEeDJibYX0ZY51KGW6cILld5Nqd25iYYSgNVAhfG9iMUCwJI5O	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTh2MEexOkc,OjF6NEC3NVY9N2F-
CFBE41o	MX7LbY5ie2ViQYPzZZk>	NV3LSJJCPSEQvF2=		MX3EUXNQ	MoXZSI94diC{ZXf1cIF1cW:wIH;mJGhFSUN5IHH0JFAvOiC2bzC1JJVO	M2D6dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUi0PVU5Lz5{MUm4OFk2QDxxYU6=
CFBE41o	NWfp[4tvTnWwY4Tpc44hSXO\|YYm=	MWCxJO69VQ>?	M3q4fVI1KGh?	M1fwWmROW09?	M{X1[3Jme3SxcnH0bY9vKG:oIFPGWHIh\GWudHHGOVA5KG23dHHueEB1emGoZnnjb4lv\yC2bzDj[YxtKHO3cn\hZ4Uh[XNiZ3z5Z49nd3Kvcx?=	MljIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7OES5OVgoRjJzOUi0PVU5RC:jPh?=
DU145	NGDxVpBMcW6jc3WgRZN{[Xl?	MnHONk42KM7:TR?=	NFLhUIszPCCq	MXLEUXNQ	MX7Jcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO\|ZXSgZZMhfXC{ZXf1cIF1cW:wIH;mJJAzOXejZkGgdJJwfGWrbjDlfJBz\XO\|aX;u	M4DkUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[wNVY3Lz5{MkK2NFE3PjxxYU6=
DU145	MoHtT4lv[XOnIFHzd4F6	M4PZfVUh\|ryP	MkDtNlQhcA>?	MojNSG1UVw>?	MoXYTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W\|c3XkJIF{KGirc4TvcoUhUDRiYXPleJlt[XSrb36=	NWrSTJpXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlAyPjZpPkKyNlYxOTZ4PD;hQi=>
DU145	NWf4[pd{U2mwYYPlJGF{e2G7	Mkj3OUDPxE1?	NYeycm9vOjRiaB?=	NWe3TYJ7TE2VTx?=	MoKxTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W\|c3XkJIF{KGirc4TvcoUhUDNiaInw[ZJi[2W2eXzheIlwdg>?	NV3Tb4VtRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlAyPjZpPkKyNlYxOTZ4PD;hQi=>
786-0	MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NVywXIhKPi5{IN88US=>	M17YXFczKGh?	NX3sSJlpTE2VTx?=	NVjqO4pOT0l3ME2zMlIh\|ryP	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
A498	NWHtTJFxT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M1rIXFYvOiEQvF2=	NIK4epQ4OiCq	NEjWNpBFVVOR	MVvHTVUxRTFwNjFOwG0>	NUGwO\|Y1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
A549/ATCC	MmO5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NVvEN3JbPi5{IN88US=>	NYDDfFY6PzJiaB?=	M1zWNWROW09?	Mn3NS2k2OD1zLk[g{txO	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
ACHN	MlHLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MlnNOk4zKM7:TR?=	M4DjTlczKGh?	MnnLSG1UVw>?	NIP1TpVIUTVyPUGuN{DPxE1?	NVzKSGtiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
BT549	M2XsNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NGf0XZc3NjJizszN	NFPzcI44OiCq	NXvxT\|NDTE2VTx?=	MoLWS2k2OD1zLkOg{txO	MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
EKVX	MmG0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NVjpZVlEPi5{IN88US=>	MlXNO\|IhcA>?	NWDVUnBwTE2VTx?=	NEnNbI9IUTVyPUGuN{DPxE1?	NYTodWdURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
HCT15	MlzMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NUHZdFU2Pi5{IN88US=>	NFWzOXY4OiCq	M1zMe2ROW09?	MkL5S2k2OD1{LkWg{txO	NUezTJVQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
HOP92	NXj3bG9VT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MmHnOk4zKM7:TR?=	MlTFO\|IhcA>?	MkLpSG1UVw>?	M1;3O2dKPTB;NDFOwG0>	MVW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
IGROV1	NFjUelBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NF3hb2Y3NjJizszN	NU\zZmZ{PzJiaB?=	Ml7SSG1UVw>?	M2TCNmdKPTB;MT6zJO69VQ>?	NFzzRXQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
LOXIMVI	M4rofWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MVy2MlIh\|ryP	M1PHVlczKGh?	M2nHeGROW09?	NFn6U\|hIUTVyPUGg{txO	M4rMNVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
M14	NXvPOYE2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MkSwOk4zKM7:TR?=	NVn4N49pPzJiaB?=	NWPmWGpnTE2VTx?=	MoXMS2k2OD1zLkOg{txO	MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
MALME-3M	NIT1VVlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M{OxfFYvOiEQvF2=	MnG1O\|IhcA>?	NVPiN2ltTE2VTx?=	M4T3emdKPTB;MD6zNkDPxE1?	NXzyfIsxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
MCF10A	Ml3oS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NUTNTolpPi5{IN88US=>	NUnDWpBnPDhiaB?=	MY\EUXNQ	MlvKS2k2OD17Lk[g{txO	M1mwWVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
MDA-MB-435	Ml7KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MYC2MlIh\|ryP	NGHSVXY4OiCq	M2H5VGROW09?	NF3CfWpIUTVyPUCuOUDPxE1?	MlvEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
MDA-MB-468	MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MWG2MlIh\|ryP	MWK3NkBp	M2XPS2ROW09?	Mni2S2k2OD1yLk[zJO69VQ>?	M{DqZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
MOLT4	MmPpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MXG2MlIh\|ryP	M3\lOlczKGh?	M2rLdWROW09?	M4nXZmdKPTB;MD60JO69VQ>?	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
NCI-ADR-RES	MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NVS0ZphSPi5{IN88US=>	MnvWO\|IhcA>?	MmWySG1UVw>?	M4HUXGdKPTB;MD6xOkDPxE1?	NH\qTWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
NCI-H23	M1nVSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MVq2MlIh\|ryP	NFu3Zo04OiCq	MWjEUXNQ	NFr1RY9IUTVyPUGg{txO	Ml3HQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
NCI-H322M	MorsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NEjvTWM3NjJizszN	MkLNO\|IhcA>?	NIrpfndFVVOR	MWLHTVUxRTBwN{mg{txO	NGLKVYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
NCI-H460	MnjmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MWq2MlIh\|ryP	NGLDfVU4OiCq	MXzEUXNQ	MlX2S2k2OD1yLke5JO69VQ>?	M17hSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
NCI-H522	NYPrZ3hST3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M1jvcVYvOiEQvF2=	MXe3NkBp	NFfsO25FVVOR	MmPKS2k2OD1yLkWg{txO	NWnpN2hjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
OVCAR3	M2HFe2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NVTUUWFlPi5{IN88US=>	MYO3NkBp	NGrCWo9FVVOR	NX\4OY92T0l3ME2xMlMh\|ryP	M2DwcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
OVCAR4	MlfkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NVfaXGk{Pi5{IN88US=>	MnGwO\|IhcA>?	NYPuUHFiTE2VTx?=	NFHLOYJIUTVyPUSg{txO	M1i3XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
OVCAR5	NHP3ToxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NFm2Z2M3NjJizszN	NG\YRlE4OiCq	NU\yfnRkTE2VTx?=	MmP5S2k2OD1yLke5JO69VQ>?	M2T6VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
OVCAR8	NHi4TYFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MXK2MlIh\|ryP	MVG3NkBp	NUX6UHNKTE2VTx?=	NWP4N3RNT0l3ME2wMlUh\|ryP	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
RXF393	MlHYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NVj6VnQxPi5{IN88US=>	MkT3O\|IhcA>?	MYnEUXNQ	MoLaS2k2OD1zLkOg{txO	NFXF[ZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
SF268	NHftS5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NG\rUHk3NjJizszN	MknzO\|IhcA>?	MUTEUXNQ	NXjiNIRHT0l3ME2xMlYh\|ryP	NXXRc\|RNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
SF295	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NUfDPG9tPi5{IN88US=>	MXq3NkBp	M2K0TmROW09?	M{n1VGdKPTB;MT62JO69VQ>?	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
SF539	NVG5bIp1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NVvIcpVGPi5{IN88US=>	NUL6OXJHPzJiaB?=	MnnGSG1UVw>?	MVrHTVUxRTJizszN	MWi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
SK-MEL-2	MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NV3nN3gyPi5{IN88US=>	NEHxb2o4OiCq	NXvocZd5TE2VTx?=	MV\HTVUxRTFwMzFOwG0>	NIrCXos9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
SK-MEL-28	NIDZO2FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NV7oeXBYPi5{IN88US=>	NF;m[WE4OiCq	M174PWROW09?	NX;QWGtLT0l3ME2xJO69VQ>?	MlvOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
SK-MEL-5	NHLB[IRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MnzpOk4zKM7:TR?=	MW[3NkBp	MYXEUXNQ	MYDHTVUxRTBwNkOg{txO	NGXDPGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
SKOV3	MlmyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M4f3S\|YvOiEQvF2=	NWDCT\|BqPzJiaB?=	M1fmUGROW09?	M33rZmdKPTB;MTFOwG0>	Mn7mQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
SN12C	NV;zcpVbT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MWG2MlIh\|ryP	Ml\HO\|IhcA>?	NWPWTllyTE2VTx?=	NXTQTYdZT0l3ME2yJO69VQ>?	NWnzOoVTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
SNB19	M{DOd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NXy5XHN6Pi5{IN88US=>	NFHIN4Q4OiCq	M2i2dWROW09?	Ml3YS2k2OD1yLk[zJO69VQ>?	NVLWTZN7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
SNB75	M2mzVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NV3ocIliPi5{IN88US=>	NFfLUm84OiCq	NF23N3ZFVVOR	NV2zU4l1T0l3ME2wMlc6KM7:TR?=	NWP1OGlKRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
SR	M3HDXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M{\nXVYvOiEQvF2=	NIrT[WY4OiCq	M4LzUWROW09?	NGLYfJFIUTVyPUCuOEDPxE1?	NFr3RWI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
T47D	NW\pNXdvT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NFSzXlk3NjJizszN	NGjSZWM4OiCq	MlrRSG1UVw>?	M4SzN2dKPTB;MD61JO69VQ>?	MmPJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
TK10	MkXwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	Mmf1Ok4zKM7:TR?=	Ml3jO\|IhcA>?	MlyxSG1UVw>?	MXzHTVUxRTBwNkOg{txO	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
UACC257	NGDa[G1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	M4jDclYvOiEQvF2=	MUG3NkBp	NVrzNpViTE2VTx?=	MYDHTVUxRTBwNTFOwG0>	NIK5N5U9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
UACC62	MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NV6yRVVWPi5{IN88US=>	M2iwO\|czKGh?	MnPBSG1UVw>?	Mn7ZS2k2OD1yLkSg{txO	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
UO31	M2TRd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NXvUUo9IPi5{IN88US=>	Mk\hO\|IhcA>?	NETOZlJFVVOR	MYHHTVUxRTBwNTFOwG0>	NEniSYw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
Hep3B	MoDWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MmLJOFghcA>?	M{DuO2ROW09?	MUHHTVUxRTBwNkmg{txO	M2PCZ\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO5PFY{Lz5{MkSzPVg3OzxxYU6=
BE(2)-C	NX\0b3pOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NWnUXXhOPzJiaB?=	MkKySG1UVw>?	NHjrPHdKSzdyPUKg{txO	NHPJTZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkmzNlMyPid-MkK5N\|I{OTZ:L3G+
Bel7402	MnTwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		M13JPVczKGh?	NEL0SlRFVVOR	MWPHTVkxRTF5IN88US=>	MXG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MUO3Okc,OjNyNkGzO\|Y9N2F-
Bel7404	Mlz3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MVq3NkBp	NHfzNnJFVVOR	MojVS2k6OD1zODFOwG0>	Mo\CQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkGzO\|YoRjJ\|ME[xN\|c3RC:jPh?=
BGC823	MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		M1Hl[lczKGh?	MYDEUXNQ	NV\qR21ZT0l7ME23MlUh\|ryP	MlvVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkGzO\|YoRjJ\|ME[xN\|c3RC:jPh?=
MGC803	M2XIUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		MXm3NkBp	NUjlT2tETE2VTx?=	NHTVeo5IUTlyPUGwMlgh\|ryP	MkXJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkGzO\|YoRjJ\|ME[xN\|c3RC:jPh?=
SGC7901	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NIrRSI04OiCq	M3nDcGROW09?	NYrTenFwT0l7ME25MlQh\|ryP	NIe2cFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
SKHEP1	NXS1ToRRT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NYHVXnpIPzJiaB?=	NEXOZ2lFVVOR	NWP5Z5E4T0l7ME2xO{DPxE1?	M3v3NVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|ME[xN\|c3Lz5{M{C2NVM4PjxxYU6=
SMMC7721	M{LKNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		MlLWO\|IhcA>?	MWXEUXNQ	MUfHTVkxRTJyIN88US=>	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MUO3Okc,OjNyNkGzO\|Y9N2F-
NUGC3	NIfVNnlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MVuxNEDPxE1?			NFrxdmRIUT1{Lkm0JO69VQ>?	NXX0XHFbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlM{OzJpPkKzNVY{OzN{PD;hQi=>
A2780 CisR	MkDiT4lv[XOnIFHzd4F6		M{PRfFE5KGh?		NF23RpRKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR\|UxKG:oIECuOFQh\|ryP	MoDRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ\|MkWyOlA{RC:jPh?=
CAL27 sens	NGfvb\|hMcW6jc3WgRZN{[Xl?		MnH6NVghcA>?		NGfLS5VKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR\|UxKG:oIECuN\|Yh\|ryP	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
CAL27 CisR	MYnLbY5ie2ViQYPzZZk>		NHrUXHgyQCCq		MV;Jcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNkGg{txO	NE\rNWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{K1NlYxOyd-MkOyOVI3ODN:L3G+
KYSE-510 CisR	NX;nZZFDU2mwYYPlJGF{e2G7		MVuxPEBp		NVHwbHNQUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlU6KM7:TR?=	NUjjT3hZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB\|PD;hQi=>
KYSE-510 sens	NEXYW5pMcW6jc3WgRZN{[Xl?		MmO4NVghcA>?		MXjJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNzFOwG0>	MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
MDA-MB-231 sens	NHe3[IFMcW6jc3WgRZN{[Xl?		Mmq2NVghcA>?		MmnYTY5pcWKrdHnvckBw\iCKRFHDJJdqfGhiSVO1NEBw\iByLk[xJO69VQ>?	NUDSPWlJRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB\|PD;hQi=>
MDA-MB-231 CisR	Moj0T4lv[XOnIFHzd4F6		MoHaNVghcA>?		NVz0[XBpUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlYyKM7:TR?=	Ml;qQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ\|MkWyOlA{RC:jPh?=
ES2	M2LNNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		MlvsOFghcA>?	MULEUXNQ	NITrS5ZKSzVyPUGyMlch\|ryP	NVnrdYNRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVM1PDlpPkKzOFk{PDR7PD;hQi=>
MDA-MB-231	MV\LbY5ie2ViQYPzZZk>	MlixNVAh\|ryP	NXPMWo81OzBibXnu	NGrUTIZFVVOR	MWnJcohq[mm2aX;uJI9nKEiGQVOyZS=>	M37UflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NEmzOFQ6Lz5{M{S5N\|Q1QTxxYU6=
MDA-MB-231	M3zjeGtqdmG\|ZTDBd5NigQ>?	MlzzNUDPxE1?	MV:yOEBp	NGL2XVJFVVOR	NIr5WYlKdmirYnn0bY9vKG:oIFjERWM3KGG\|c3Xzd4VlKGG\|IHHj[ZR6dGG2aX;uJIxmfmWuczDv[kB1fWK3bHnu	MnTPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN2OUO0OFkoRjJ\|NEmzOFQ6RC:jPh?=
HS68	MlfpR5l1d3SxeHnjJGF{e2G7	NFrLemUyOCEQvF2=	NW\zNIhqPzJiaB?=	NITtWWVFVVOR	NWrKWoJ4UUN3ME20MlYh\|ryP	NV7JeFY6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1O\|A2PDJpPkKzOVcxPTR{PD;hQi=>
MEF	NXjZZ\|RtS3m2b4TvfIlkKEG\|c3H5	MV2xNEDPxE1?	M3LzNVczKGh?	MmHlSG1UVw>?	MmHMTWM2OD12LkSg{txO	NVz3NFJQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1O\|A2PDJpPkKzOVcxPTR{PD;hQi=>
BGC823	M1;vfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7			M1vaZ2ROW09?	M3jMfWdKPTB;Mz6wPUDPxE1?	M3vDdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NkCxO\|A3Lz5{M{[wNVcxPjxxYU6=
High5	MX3LbY5ie2ViQYPzZZk>		M3fNVVI1KGh?		NW\uZY1uUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEPiC5aYToJGlEPTBib3[gNE4xQSEQvF2=	Mli3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4MEK1NlMoRjJ\|NkCyOVI{RC:jPh?=
High5	M1eyemtqdmG\|ZTDBd5NigQ>?		M{jQRVI1KGh?		MYDJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVOxJJdqfGhiSVO1NEBw\iByLkGyJO69VQ>?	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZyMkWyN{c,OjN4MEK1NlM9N2F-
High5	M3jUUGtqdmG\|ZTDBd5NigQ>?		MnvXNlQhcA>?		MU\Jcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVOzJJdqfGhiSVO1NEBw\iByLkG3JO69VQ>?	NVjNXXl7RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NFI2OjNpPkKzOlAzPTJ\|PD;hQi=>
A549	MkX1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?			M3zJeGROW09?	M{CwNWlEPTB;MT6wO\|kh\|ryP	NGXZOI09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[0OFIyOCd-MkO2OFQzOTB:L3G+
HCT116	MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MlPlSG1UVw>?	MnvsTWM2OD1yLke1O{DPxE1?	NWDIWml3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2OFQzOTBpPkKzOlQ1OjFyPD;hQi=>
K562	NYfre\|VPT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			MXfEUXNQ	MoL4TWM2OD1yLk[0OUDPxE1?	MkTKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NESyNVAoRjJ\|NkS0NlExRC:jPh?=
GBC-SD	M{TMcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MW[1JO69VQ>?	NHnUdHM4OiCq	MnXzSG1UVw>?	MUXJR\|UxRTF2Lkig{txO	MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyN{K2NEc,OjN5MEeyOlA9N2F-
HGC27	M{\PWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MVS1JO69VQ>?	NELwWHI4OiCq	MW\EUXNQ	NFvBdFdKSzVyPUWuOEDPxE1?	NGnVNIo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ewO\|I3OCd-MkO3NFczPjB:L3G+
HL60	NH\EZYNMcW6jc3WgRZN{[Xl?	MlfEOUDPxE1?	NXrLOFlMOjRiaB?=	MkjVSG1UVw>?	M1frRmlvcGmkaYTpc44hd2ZiSFTBRy=>	NHzHd3M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{ewO\|I3OCd-MkO3NFczPjB:L3G+
Vero	MmLqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MXm3NkBp	NWPk[Is3TE2VTx?=	M{TKbGlEPTB;MD65JO69VQ>?	NFrISpk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e4OlQ2Oid-MkO3PFY1PTJ:L3G+
NCI60	NVnqSZhXS3m2b4TvfIlkKEG\|c3H5		MnvmOFghcA>?	M{XGVGROW09?	M3jVSGdKRTBwNUOg{txO	MoHqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUWzNlcoRjJ2MEG1N\|I4RC:jPh?=
SW620	MY\DfZRwfG:6aXOgRZN{[Xl?		MkTGOFghcA>?	MlPOSG1UVw>?	NYWwWJZGT0l;MD61OEDPxE1?	M1X0S\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEG1N\|I4Lz5{NECxOVMzPzxxYU6=
U251	M3PKbGN6fG:2b4jpZ{BCe3OjeR?=		NXLlZVI{PDhiaB?=	Mm\1SG1UVw>?	MVvHTV0yNjV\|IN88US=>	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDBzNUOyO{c,OjRyMUWzNlc9N2F-
KM12	M4XRdWN6fG:2b4jpZ{BCe3OjeR?=		MmjGOFghcA>?	NHHwXVlFVVOR	NYHxRWJoT0l;MT64PEDPxE1?	NEXz[GE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECxOVMzPyd-MkSwNVU{Ojd:L3G+
Hs578T	M3TPfWN6fG:2b4jpZ{BCe3OjeR?=		NHSxe5Y1QCCq	NGP1bmhFVVOR	M1zCXWdKRTRwOEOg{txO	M{mwNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEG1N\|I4Lz5{NECxOVMzPzxxYU6=
MDA-MB-231	NWHOS\|ZOT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NEjxR2M1QCCq		M1;leWlEPTB;MD6xOkDPxE1?	MVK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDB7NUCxOkc,OjRyOUWwNVY9N2F-
MCF7	NH7nTolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		MX:0PEBp		MWTHTV0xNjR3IN88US=>	NVn0TItERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNVk2PTVpPkK0NVE6PTV3PD;hQi=>
MDA-MB-435	NY[5fWxST3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NHPuTYQ1QCCq		MkTMS2k:OC56ODFOwG0>	NV[yWVJzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNVk2PTVpPkK0NVE6PTV3PD;hQi=>
AsPC1	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXezNEDPxE1?	MlLTOFghcA>?	MUHEUXNQ	MkfVTWM2OD1\|Lk[2JO69VQ>?	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF6NUO3PEc,OjRzOEWzO\|g9N2F-
Jurkat E6.1	M{\NW2tqdmG\|ZTDBd5NigQ>?		NXXZfYNNOiCq	NH2wTnBFVVOR	NWHCTJZ[UW6qaXLpeIlwdiCxZjDISGFEPCC5aYToJGlEPTBib3[gNE4xPCEQvF2=	NVnNOpV{RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOlE5PjJpPkK0NlYyQDZ{PD;hQi=>
KG1	NF\0TolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		M3jwcFQ5KGh?		NGr1NYFKSzVyPUGuNVUh\|ryP	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV{NUCwN{c,OjR3MkWwNFM9N2F-
HEL	MkTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MUS0PEBp	MorUSG1UVw>?	NInWNIZKSzVyPUCuOFkh\|ryP	MYC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDZ7NEC1OUc,OjR4OUSwOVU9N2F-
HSC3	MXXDfZRwfG:6aXOgRZN{[Xl?		MlHlNVIhcA>?	NGHNTnVFVVOR	MmPoTWM2OD1yLkexJO69VQ>?	NIDicJM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2OlU3OCd-MkS3OlY2PjB:L3G+
HT-29	MY\DfZRwfG:6aXOgRZN{[Xl?		NYrQTlhGOTJiaB?=	NVnUXWF5TE2VTx?=	M1;zbGlEPTB;MD63NkDPxE1?	MoHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5Nk[1OlAoRjJ2N{[2OVYxRC:jPh?=
KB	NXHSSYE2S3m2b4TvfIlkKEG\|c3H5		M{LOVlEzKGh?	MlzoSG1UVw>?	MknMTWM2OD1yLke1JO69VQ>?	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4NkW2NEc,OjR5Nk[1OlA9N2F-
H460	MlP4R5l1d3SxeHnjJGF{e2G7		M1HPWFEzKGh?	MVrEUXNQ	NXTtdo1EUUN3ME2wMlgzKM7:TR?=	MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4NkW2NEc,OjR5Nk[1OlA9N2F-
MONE1	MYPDfZRwfG:6aXOgRZN{[Xl?		Ml\KNVIhcA>?	NWi2e5dPTE2VTx?=	NYLmVWt3UUN3ME2xMlE5KM7:TR?=	NYHxWWtuRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OlY2PjBpPkK0O\|Y3PTZyPD;hQi=>
MKN45	M2HkPGN6fG:2b4jpZ{BCe3OjeR?=		Mne4NVIhcA>?	NUnuNGQ1TE2VTx?=	NUKzOG56UUN3ME2xMlI2KM7:TR?=	NWDub3I5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OlY2PjBpPkK0O\|Y3PTZyPD;hQi=>
TSGH	NGX3[3ZEgXSxdH;4bYMhSXO\|YYm=		MV6xNkBp	M37RfWROW09?	MVHJR\|UxRTJwMEmg{txO	MnXJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5Nk[1OlAoRjJ2N{[2OVYxRC:jPh?=
HL60	Mn6zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MkjlO\|IhcA>?	MUjEUXNQ	MV;HTVUxRTBwOUOg{txO	NVnxVJZQRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA2ODBpPkK0PVAxPTByPD;hQi=>
HEK293	M33qZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7		MnP0O\|IhcA>?	NET0[XNFVVOR	M{\yV2dKPTB;MUOg{txO	Mlf6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC1NFAoRjJ2OUCwOVAxRC:jPh?=
Jurkat	NELNUoZEgXSxdH;4bYMhSXO\|YYm=	MlXuNVAxKM7:TR?=	M1;aW\|czKGh?		MoHnS2k2OD1yLkmg{txO	NFHDZ289[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFc1Oyd-MkS5NFA4PDN:L3G+
GM15850	NUjIVXBJU2mwYYPlJGF{e2G7	MUWyMlUh\|ryP	NV\yOYJ3QTZiaB?=	M3rTUGROW09?	M4fOb4Rw\XNibn;0JIlvcGmkaYSgTGRCSyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBpcXO2b37lJGg{KGy7c3nu[UAyPCCjY3X0fYxifGmxbjDv[kBHYE5iZ3Xu[S=>	MmToQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7MkGzOlcoRjF4OUKxN\|Y4RC:jPh?=
GM15850	Mn;XT4lv[XOnIFHzd4F6	M4qxeFIvPSEQvF2=	MmTEPVYhcA>?	M4HKRmROW09?	NEfCbmtld2W\|IH7veEBqdmirYnn0JGhFSUNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hcGm\|dH;u[UBJPCCueYPpcoUhPSCjY3X0fYxifGmxbjDv[kBHYE5iZ3Xu[S=>	NUnUSIZRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5NlE{PjdpPkG2PVIyOzZ5PD;hQi=>
GM15850	M17xSGtqdmG\|ZTDBd5NigQ>?	MYSyMlUh\|ryP	NVjnNoNvQTZiaB?=	MUHEUXNQ	Mm\x[I9meyCwb4SgbY5pcWKrdDDISGFEKGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFQhdHm\|aX7lJFEzKGGlZYT5cIF1cW:wIH;mJGZZViCpZX7l	NYTOfmRGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5NlE{PjdpPkG2PVIyOzZ5PD;hQi=>
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NCI-H630	MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MorETWM2OD12Lke4NFgyKM7:TR?=	M2TvVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
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HCC1569	M2nicGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M4P6UWlEPTB;ND64PFQ2QCEQvF2=	NH;t[lE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
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ZR-75-30	MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MkK5TWM2OD1zOT6wPVY5KM7:TR?=	MnzWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
LU-135	NUXGRoJ{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NV;SRmdlUUN3ME2xPU4zPjN4IN88US=>	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
NCI-H2029	NHX4S4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NVPsdFlWUUN3ME2xPU43ODJzIN88US=>	MlHvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
HT-1197	NUnrZZp5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MmP2TWM2OD1zOT65NlI4KM7:TR?=	M3zyXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
IGR-1	M16zcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MYLJR\|UxRTJyLkOxNVch\|ryP	MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
U-87-MG	MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M{S1ZmlEPTB;MkCuOlQyOyEQvF2=	M{HMcVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H1304	MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NEXiUpBKSzVyPUKwMlc2ODFizszN	M3npXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
RCC10RGB	NFXyfnJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MmraTWM2OD1{MT6yPFI6KM7:TR?=	NX7MO\|Z6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
NCI-H520	MlixS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NInXeIZKSzVyPUKyMlc3OTFizszN	Mnu1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
SK-LU-1	M{HYOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NV;NNZJlUUN3ME2yN{4xPzF6IN88US=>	NH;EV3I9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
UACC-893	M2Pmd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MXLJR\|UxRTJ\|LkGwN\|gh\|ryP	NWnzeng3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
NCI-H2347	MoTyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MkPmTWM2OD1{Mz6yOlc1KM7:TR?=	NGGwT4Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
U-118-MG	MmH4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NXfsU5Y5UUN3ME2yN{44OjF5IN88US=>	NGLOPIg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NCI-H596	NXXne4tGT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MXvJR\|UxRTJ\|LkmwOVUh\|ryP	NETnWpg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
MZ1-PC	NV;xeZY5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MkP2TWM2OD1{ND62O\|U6KM7:TR?=	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
SW1417	MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NGO5RXBKSzVyPUK1MlI1QThizszN	Mmj3QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NCI-H82	MlvLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NWexXIVrUUN3ME2yOk41Ojh6IN88US=>	NU\yZoptRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
COLO-678	M4rkRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NFP2O5JKSzVyPUK3MlQ6OTlizszN	NVS5OnNyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
KURAMOCHI	M1PGUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				Mo\pTWM2OD1{OD6xNlYh\|ryP	NHPrOlI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NCI-H1650	NEXzUm1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MWnJR\|UxRTNyLkG5NlQh\|ryP	NGrIVmU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
HCC1954	NF\ocItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				M2q0cmlEPTB;M{GuN\|Y3PiEQvF2=	MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
GAK	M1;GPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MUTJR\|UxRTN{LkK2NVYh\|ryP	NIfi[nQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
YAPC	M13hXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NVHPeJJyUUN3ME2zN{44PjR6IN88US=>	NYH3dWxDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
NCI-H2228	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M{\U[mlEPTB;M{SuN\|YxPSEQvF2=	M3jCdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
VMRC-RCZ	NXvH[nJLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MULJR\|UxRTN4LkOwNlIh\|ryP	M3ywclxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NMC-G1	M2rOXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NFvYNGpKSzVyPUO3MlA2OjlizszN	M2KzeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
D-336MG	MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NHmyPY9KSzVyPUSxMlg1PDJizszN	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
UACC-62	MmG0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M3L1WmlEPTB;NEKuOVUxPSEQvF2=	NHj6d5M9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
C-33-A	Ml24S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MXvJR\|UxRTR3LkOyOVYh\|ryP	M{LFdFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
HCC1937	MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M4LI[2lEPTB;NEeuNVk2QCEQvF2=	M3mwS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
HDLM-2	NUXhbplMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MV;JR\|UxRTR5LkKyPFch\|ryP	MYm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
NCI-H727	MmrSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MWjJR\|UxRTR5LkK0OVEh\|ryP	MlfOQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NY	MnjHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NGr0NZJKSzVyPUS5MlM6ODhizszN	NUnlPWZRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
NCI-H2291	M4TJSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MYrJR\|UxRTR7LkizNFEh\|ryP	NILUeIw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Ac-Histone H4 / Ac-Histone H3; PubMed: 21598070 J Neurooncol D54 and U87 glioma cells were treated with lower concentrations (1-3 µM) of vorinostat. The effects on histone acetylation was assessed by immunoblotting. phospho-CDK1; PubMed: 21598070 Changes in level of inhibitory phosphorylation of cdk1 after treatment with vorinostat was determined at 24 and 48 h (using a Tyr-15 residue phospho-specific antibody) along with total cdk1 as loading control. phospho-Cdc25c / Cdc25c; PubMed: 21598070 J Neurooncol Time course of changes in phospho-cdc25c levels after exposure to vorinostat (5 µM). Cyclin B1; PubMed: 21598070 J Neurooncol Cyclin B1 levels were determined in D54 and U87 cells after treatment with various doses of vorinostat at 24 and 48 h. c-Myc / c-Raf / Akt / Cyclin D1 / CDK4; PubMed: 19440035 Cancer Biol Ther Cell lysates from JeKo-1 and Mo2058 cells exposed to vorinostat were immunoblotted for c-Myc, c-Raf, AKT, cyclin D1, CDK4 and acetylated histone H3. The levels of β-actin served as the loading control. p-eIF2α / ATF4 / CHOP ; PubMed: 20072656 Neoplasia Western blot was performed to evaluate activated eIF2α (P-eIF2α), translational up-regulation of ATF4, and CHOP expression in U251 cells after vorinostat exposure. p21; PubMed: 21598070 J Neurooncol To determine the dose response relationship to vorinostat, cells were exposed to various higher concentrations (5–10 µM) of the agent and the levels of p21 determined by immunoblotting.	21598070 19440035 20072656
Immunofluorescence	HDAC1 / CK19; PubMed: 29917299 Cancer Sci Immunocytochemistry to detect the expression of HDAC1 and CK 19 in PDAC cells with/without Vorinostat treatment. HDAC1 and CK19 were stained red and green, respectively. The Hoechst‐stained nuclei appear blue. Scale bar: 100 μm. The CK19 expression in PSN1 and MiaPaCa2 was increased by Vorinostat treatment. Nrf2; PubMed: 20566897 Blood Vorinostat induced translocation of Nrf2 from cytosol to nucleus. U937 cells were treated with 2μM vorinostat for 18 hours and then cytospun. Cells were fixed and labeled for Nrf2 protein (red) and nucleus (blue, 4,6-diamidino-2-phenylindole) as described in “Immunofluorescence and confocal microscopy.” Images were taken by Nikon Eclipse TE2000 confocal microscope with 40×/1.30 numeric aperture oil objective lens. Ctrl indicates control cells without treatment; and Vor, vorinostat. α-SMA; PubMed: 26330748 Mol Vis Representative immunofluorescence images and the quantification graph showing the effect of vorinostat (2.5 µM), TGIF1, and TGIF2 siRNA knockdown on αSMA. Control human corneal fibroblast cultures (-TGFβ) showed no αSMA staining. Cultures treated with TGFβ1 (5 ng/ml) showed a robust αSMA staining. Vorinostat treatment markedly attenuated TGFβ-evoked αSMA staining. The knockdown of TGIF1 (+TGFβ+vorinostat+TGIF1siRNA) and TGIF2 alone (+TGFβ+vorinostat+TGIF2siRNA) or in combination (+TGFβ +vorinostat +TGIF1 +2siRNA) markedly blocked the effect of vorinostat on αSMA. Scale bar=100 μm. Ac-STAT3 / IGF2; PubMed: 27086926 Oncogene Immunofluorescence staining of Ac-STAT3 and IGF2 after vorinostat treatment.	29917299 20566897 26330748 27086926
Growth inhibition assay	Cell apoptosis; PubMed: 19440035 Cancer Biol Ther Cells were treated with indicated concentrations of vorinostat for 48 hours. Following this, the percentage of annexin-V-stained apoptotic cells was determined by flow cytometry. Values represented as bar graphs are the mean of 3 experiments plus or minus the standard error (SE) Cell viability; PubMed: 27086926 Oncogene The effects of vorinostat on the viability of the indicated NSCLC cell lines and their corresponding acquired VoR sublines. Cell viability was determined by the MTT assay (n = 3).	19440035 27086926
ELISA	IGF2; PubMed: 27086926 Oncogene Determination of vorinostat-induced IGF2 production by ELISA (n = 3). The conditioned mediums (CMs) obtained from cells treated with vorinostat (5 μM) for 2 days were used for ELISA. IL-13 / IL-10 / IL-5; PubMed: 18541724 Blood Effect of vorinostat on TH1/TH2 cytokine secretion in HL cell lines. Cells were incubated with medium or vorinostat (5 μM) for 24 hours and supernatants were examined for cytokine levels by ELISA. Each value represents a mean of 3 independent experiments done in triplicate (± SEM). M-CSF; PubMed: 23042302 Carcinogenesis Primary PyMT tumor cells were treated with SAHA (0-1000nM) for varying time points (8–48h), and supernatants were assayed by ELISA for M-CSF secretion. P < 0.05 and P < 0.001 versus control treatment. MMP-9; PubMed: 23042302 Carcinogenesis Primary PyMT tumor cells were treated with SAHA (0-1000nM) for varying time points (8–48h), and supernatants were assayed by ELISA for MMP-9 secretion. P < 0.05 and **P < 0.001 versus control treatment.	27086926 18541724 23042302

In vivo

Administration of Vorinostat (~100 mg/kg/day) significantly inhibits the growth of CWR22 human prostate xenografts in nude mice with tumor reductions of 78%, 97% and 97%, at doses of 25 mg/kg/day, 50 mg/kg/day and 100 mg/kg/day, respectively, compared with control. Vorinostat induces the accumulation of acetylated core histones and prostate-specific antigen mRNA expression in CWR22 cells, resulting in higher levels of serum prostate-specific antigen than predicted from tumor volume alone. ^[2] Oral administration of Vorinostat (0.67g/L) crosses the blood-brain barrier, increases histone acetylation in the brain, and dramatically improves the motor impairment in the R6/2 mice model of Huntington's disease. ^[5]

Protocol

Kinase Assay: ^[1]	- Collapse Immunoprecipitation-HDAC assays: The lysate of Jurkat cells is incubated for 1 hour on ice and cleared by centrifugation at 12,000 g for 10 minutes at 4 °C. Supernatants are precleared with 30 μL of 50% protein G-Sepharose slurry for 1 hour at 4 °C. Beads are pelleted by centrifugation and supernatants are incubated for 1 hour at 4 °C with 10 μg of IgG fraction from anti-HDAC1 or HDAC3 polyclonal antisera (preincubated 2 hours at room temperature with either the homologous or heterologous immunizing peptide). Both antisera are raised in rabbits against the carboxylterminal peptide of HDAC1 and HDAC3 by using synthetic peptides coupled to keyhole limpet hemocyanin. 30 μL of 50% protein G-Sepharose slurry is added for 1 hour at 4 °C. Immune complexes are pelleted by centrifugation and washed three times with 1 mL of lysis buffer. Beads are resuspended in 200 μL of HDAC buffer (20 mM Tris-HCl, pH 8.0/150 mM NaCl/10% glycerol), and the HDAC assay is performed with an ³H-acetylated peptide corresponding to amino acids 1-24 of histone H4. Released [³H]acetic acid is quantified by scintillation counting. For inhibitions studies, the immunoprecipitated complexes are preincubated with the different concentrations of Vorinostat for 30 minutes at 4 °C.
Cell Research: ^[2]	- Collapse Cell lines: LNCaP, PC-3, and TSU-Pr1 Concentrations: Dissolved in DMSO, final concentrations ~7.5 μM Incubation Time: 1, 2, 3 and 4 days Method: Cells are exposed to various concentrations of Vorinostat for 1, 2, 3 and 4 days. Cell viability is assessed by trypan blue dye exclusion. (Only for Reference)
Animal Research: ^[2]	- Collapse Animal Models: Male BALB/c nude (nu/nu) mice implanted with CWR22 tumor cells Dosages: ~100 mg/kg/day Administration: Injection i.p. (Only for Reference)

References

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Solubility (25°C)

In vitro	DMSO	52 mg/mL (196.74 mM)
	Water	Insoluble
	Ethanol	'''3 mg/mL warmed
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 2% DMSO+30% PEG 300+5% Tween 80+ddH2O For best results, use promptly after mixing.	5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Vorinostat (SAHA) SDF

Molecular Weight	264.3
Formula	C₁₄H₂₀N₂O₃
CAS No.	149647-78-9
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	MK0683, Suberoylanilide hydroxamic acid
Smiles	C1=CC=C(C=C1)NC(=O)CCCCCCC(=O)NO

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Dosage

mg/kg

Average weight of animals

Dosing volume per animal

Number of animals

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO+ % + % Tween 80+ % ddH₂O

Calculate Reset

Calculation results:

Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-05-17)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03022565	Withdrawn	Drug: Vorinostat	Uveal Melanoma	University of Miami\|University of Miami Sylvester Comprehensive Cancer Center	January 2020	Early Phase 1
NCT03167437	Recruiting	Drug: Vorinostat\|Drug: Ustekinumab	Crohn''s Disease	National Institute of Allergy and Infectious Diseases (NIAID)\|National Institutes of Health Clinical Center (CC)	October 30 2017	Phase 1\|Phase 2
NCT03056495	Recruiting	Drug: N-hydroxy-N''-phenyl-octanediamide (Vorinostat)	Alzheimer Disease	German Center for Neurodegenerative Diseases (DZNE)\|University Hospital Bonn\|University of Göttingen	September 28 2017	Phase 1
NCT04308330	Recruiting	Drug: Vorinostat	Ewing Sarcoma\|Rhabdomyosarcoma\|Wilms Tumor\|Neuroblastoma\|Hepatoblastoma\|Germ Cell Tumor	New York Medical College	March 17 2017	Phase 1
NCT02836548	Unknown status	Drug: Vorinostat	Melanoma\|Skin Neoplasms	The Netherlands Cancer Institute	June 2016	Phase 1\|Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
What’s the recommendation about reconstitution of the compound for in vivo animal study?
Answer:
We recommend the following vehicle for S1047: 2% DMSO, 40% PEG 300, 5% Propylene glycol, 1% Tween 80 at 5 mg/mL.

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

Selective HDAC Inhibitor

RGFP966 : HDAC3-selective, IC50=80 nM.
Selective HDAC Inhibitor

Rocilinostat (ACY-1215) : HDAC5-selective, IC50=5 nM.
Most Potent HDAC Inhibitor

Quisinostat (JNJ-26481585) : HDAC1, IC50=0.11 nM; HDAC2, IC50=0.33 nM.
FDA-approved HDAC Inhibitor

Vorinostat (SAHA, MK0683) : Approved by FDA against cutaneous T cell lymphoma.
Newest HDAC Inhibitor

RG2833 (RGFP109) ^New : Brain-penetrant HDAC inhibitor with IC50 of 60 nM and 50 nM for HDAC1 and HDAC3, respectively.

Related HDAC Products

LMK-235 ^New

LMK-235 is a selective inhibitor of HDAC4 and HDAC5 with IC50 of 11.9 nM and 4.2 nM, respectively.
CAY10603 ^New

CAY10603 (BML-281) is a potent and selective HDAC6 inhibitor with IC50 of 2 pM, >200-fold selectivity over other HDACs.
Domatinostat (4SC-202) ^New

Domatinostat (4SC-202) is a selective class I HDAC inhibitor with IC50 of 1.20 μM, 1.12 μM, and 0.57 μM for HDAC1, HDAC2, and HDAC3, respectively. Also displays inhibitory activity against Lysine specific demethylase 1 (LSD1). Phase 1.
Panobinostat (LBH589)

Panobinostat (LBH589, NVP-LBH589) is a novel broad-spectrum HDAC inhibitor with IC50 of 5 nM in a cell-free assay. Panobinostat (LBH589) induces autophagy and apoptosis. Panobinostat effectively disrupts HIV latency in vivo. Phase 3.
Entinostat (MS-275)

Entinostat (MS-275, SNDX-275) strongly inhibits HDAC1 and HDAC3 with IC50 of 0.51 μM and 1.7 μM in cell-free assays, compared with HDACs 4, 6, 8, and 10. Entinostat induces autophagy and apoptosis. Phase 3.
Trichostatin A (TSA)

Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.
Romidepsin (FK228, Depsipeptide)

Romidepsin (FK228, Depsipeptide, FR 901228, NSC 630176) is a potent HDAC1 and HDAC2 inhibitor with IC50 of 36 nM and 47 nM in cell-free assays, respectively. Romidepsin (FK228/depsipeptide) controls growth and induces apoptosis in neuroblastoma tumor cells.

Features:More effective than other classical HDAC inhibitors such as TSA, TPX, and butyrate.
RGFP966

RGFP966 is an HDAC3 inhibitor with IC50 of 0.08 μM in cell-free assay, exhibits > 200-fold selectivity over other HDAC.
Tubastatin A

Tubastatin A is a potent and selective HDAC6 inhibitor with IC50 of 15 nM in a cell-free assay. It is selective against all the other isozymes (1000-fold) except HDAC8 (57-fold). Tubastatin A promotes autophagy and increases apoptosis.
Mocetinostat (MGCD0103)

Mocetinostat (MGCD0103, MG0103) is a potent HDAC inhibitor with most potency for HDAC1 with IC50 of 0.15 μM in a cell-free assay, 2- to 10- fold selectivity against HDAC2, 3, and 11, and no activity to HDAC4, 5, 6, 7, and 8. Mocetinostat (MGCD0103) induces apoptosis and autophagy. Phase 2.

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By Signaling Pathways

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Vorinostat (SAHA)