Vorinostat (SAHA, MK0683)

Catalog No.S1047 Synonyms: SAHA, MK0683, suberoylanilide hydroxamic acid

Vorinostat (SAHA, MK0683) Chemical Structure

Molecular Weight(MW): 264.3

Vorinostat (suberoylanilide hydroxamic acid, SAHA) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay.

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Cited by 103 Publications

20 Customer Reviews

  • Activity of vorinostat in Jurkat and Peer T-ALL cell lines in an MTT cell viability assay. Cells were treated with increasing drug concentrations for 72 h. The data are plotted as the mean % of DMSO-treated control cells against the corresponding drug concentration. The error bars are the standard error. For each drug and cell line, 3-4 independent experiments were performed with 6 replicates at each drug concentration.

    Nature 2011 471, 235-9. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Differential effects of HDAC inhibitors on histone and tubulin acetylation. Immunofluorescence analysis of histone H3 (K9ac/K14ac) and tubulin acetylation in HeLa cells treated for 4 h with vehicle, SAHA (10 μM), tacedinaline (50 μM), PCI-24781 (20 μM. (a) Mapping of histone acetylation in K562 cells treated with HDAC inhibitors by LC-MS/MS. Cells were treated with TSA (10 μM), SAHA (5 μM), PCI-24781 (2 μM), tacedinaline (50 μM) for 6 h. Histones were extracted from cells and acetylated peptides were quantified after isobaric tagging.

    Nat Biotechnol 2011 29, 255-265. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Western blot analysis of histone H3 acetylation in the spleen of untreated and vorinostat-treated hNF-E2 tg mice (n = 4 of each genotype).

    J Exp Med 2012 209, 35-50. Vorinostat (SAHA, MK0683) purchased from Selleck.

    SAHA down-regulates c-FLIP expression and increases the sensitivity of CaP cells to undergo apoptosis in the presence of bicalutamide (A) Bar graph illustrating the sub-G0/G1 cell population in 22Rv1 (left panel) and LNCaP (right panel) cells in the absence and presence of 10μM bicalutamide, administered as a single agent or in ombination with increasing concentrations of the HDAC inhibitor, SAHA. (B) Immunoblots demonstrating the single agent activity of bicalutamide or SAHA, or effect upon their combined administration on the expression of c-FLIP and/or the processing of PARP. Equal protein loading was confirmed by GAPDH. (C) Bar graph illustrating the effect of bicalutamide and SAHA upon the induction of caspase-8 or caspase-3/7 activity in 22Rv1 cells (left panel) and LNCaP cells (right panel). (D) Immunoblots characterizing the impact of SAHA and/or bicalutamide upon the induction of PARP cleavage, in the absence or presence of the pan-caspase inhibitor, ZIETD. ZIETD was incubated with the cells for 12 h prior to the experiment. All data points shown in (A) and (C) represent the mean ± S.E.M. value, calculated from four independent experiments.

    Clin Cancer Res 2012 18, 3822-33. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Analyses of efficacy, potency and IC50 of HDAC inhibitors point toward HDACs 1–3 as relevant candidates for beta cell protection. Ranking and raw data on ITF drugs (Table 3) and commercial HDAC inhibitors (Table 4) underlying the heat maps of Fig. 1 (A and B). The HDAC inhibitor compounds were tested using a HDAC activity kit and recombinant proteins to determine IC50 values on each individual HDAC (right part of the table). Each drug was further tested using Real-Time Cell Analysis (RTCA) to score their maximal effective concentration (ECmax) as well as the corresponding rescue percentage of cytokine treated INS-1 cells. The drugs were ranked according to % rescue. * DMSO alone controls were included in each experiment, but not shown here. The results indicate that the highest concentrations of DMSO used here (1:1,000) were slightly potentiating the proliferation of the cells. The effects observed in this group of compounds were ascribed to the DMSO.?

    Diabetologia 2012 55, 2421-2431. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Suberoylanilide hydroxyamic acid (SAHA) preserves CFP (+) expression in mouse optic nerves (MONs) from older mice. (a) CFP (+) mitochondria were longer and thicker in MONs from 12-month-old Thy-1 CFP mice (inset; scale bar = 2 μm). Oxygen–glucose deprivation (OGD) consistently reduced CFP pixel intensity in 12-month-old MONs despite longer and brighter CFP ( +) mitochondria (yellow arrows). (b) Blockade of AMPA/kain ate receptors with NBQX (30 μM), or pan-Histone deacetylase (HDAC) inhibition with SAHA (5 μM), effectively preserved CFP pixel intensity during OGD. The preservation of CFP (+) mitochondria correlates with protection of the CAP area during OGD and profound recovery.

    J Neurochem 2012 123 Suppl 2, 108-15. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • D and E, Regression of IL-13Ra 2-negative pancreatic tumors treated with SAHA and/or IL-13-PE. Mice were treated daily with i.p. injection of SAHA (25 mg/kg) from day 4 after tumor implantation for two weeks followed by i.t. injection of IL-13-PE (100 μ g/kg) from day 5 for two weeks. F and G, Regression of IL-13Ra 2-posotive pancreatic tumors (HS766T and MIA-PaCa2) treated with SAHA and/or IL-13-PE. The schedule of treatment was similar as in panel D and E. Statistical significances are shown by *: P < 0.05,†: P < 0.001.

    J Transl Med 2011 9, 37. Vorinostat (SAHA, MK0683) purchased from Selleck.

    IL-13Rα2 expression is upregulated in pancreatic tumors but not in organs of mice after treatment with HDAC inhibi tor, SAHA. A, qRT-PCR of human IL-13Rα2 in implanted pancreatic tumors after SAHA and IL-13-PE treatment. Tumors were harvested next day after IL-13-PE treatment and total RNA extracted. Data shown is ratio of human IL-13Rα2/β-actin expression and multiplied by 1000 for convenience. Bars , SD of triplicate determinations. B, qRT-PCR of mouse IL-13Rα2 in mice organs after SAHA and IL-13-PE treatment. Tissues were harvested at the same time point as in panel A and total RNA extracted. Data shown is ratio of mouse IL-13Rα2/β-actin expression and multiplied by 100 for convenience. C, IHC of human IL-13Rα2 in implanted pancreatic tumors after SAHA and IL-13-PE treatment. D, IHC of mouse IL-13Rα2 in mice organs after SAHA and IL-13-PE treatment. Liver, brain, kidney, pancreas, lung and spleen were fixed for immunostaining of mouse IL-13Rα2 as visualized by Alexa555. Nucleus was counterstained by DAPI.

    J Transl Med 2011 9, 37. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Proliferation o f HT29 and HCT116 cells after 48 h of butyrate or SAHA treatment. Proliferation measurements using the xCEL Ligence RTCA DP instrument (Roche) in cells treated with increasing doses of butyrate or SAHA, compared with control medium cells (0) (*P < 0.05). The mean ?SEM of three cell culture replicates is shown.

    Mol Carcinog 2012 52, 459-74. Vorinostat (SAHA, MK0683) purchased from Selleck.

    SAHA induces the down-regulation of cFLIP expression and promotes TRAIL-mediated activation of a mitochondrial-operated apoptotic pathway. a BT-474 cells were treated as indicated in Fig. 1 and the cells were then harvested to analyse caspase-8, Bid, caspase-9, caspase-3 and PARP expression, using GAPDH as the protein loading control. b BT-474 cells were treated with SAHA for 8 h at the doses indicated and subsequently, the cells were harvested and the apoptosis-related proteins were analysed in western blots as described in the "Materials and Methods". The results shown are representative of at least three independent experiments

    Invest New Drugs 2012 30, 541-547. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Treatment with SAHA sensitizes breast cancer cells to TRAILinduced apoptosis. BT-474 and MDA-MB-231 cells were incubated for 8 h with SAHA at the doses indicated. After this time, TRAIL (500 ng/ml) was added to some of the cultures for an additional 15 h. Apoptosis was measured as the percentage of cells with subG1 DNA content as described in the "Materials and methods". The results show the mean and S.E.M. of three independent experiments

    Invest New Drugs 2012 30, 541-547. Vorinostat (SAHA, MK0683) purchased from Selleck.

    SAHA facilitates the Itch/AIP4-independent proteasomal degradation of cFLIP proteins in breast cancer cells. a BT-474 cells were treated with SAHA (5 μM) for 8 h in the presence or absence of the proteasome inhibitors MG132 or epoxomicin (at the doses indicated), and they were then harvested and the cFLIP content was analysed in western blots. b BT-474 cells were transfected with either the siRNA oligonucleotides targeting Itch/AIP4 or the non-targeting RNA oligonucleotide (scrambled) for 48 h as described in the "Materials and Methods". Subsequently, the cells were treated with SAHA (5 μM) for 8 h and then cFLIP and Itch/AIP4 expression was analysed in immunoblots

    Invest New Drugs 2012 30, 541-547. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • cFLIP down-regulation following SAHA treatment is responsible for the sensitization of breast cancer cells to TRAIL. a BT-474 cells were transfected with either different siRNAs directed against the cFLIP isoforms or the non-targeting RNA oligonucleotide (scrambled) as described in the "Materials and Methods". After 24 h, the cells were treated with TRAIL (500 ng/ml) for a further 15 h and apoptosis was then measured as the percentage of cells with subG1 DNA content. The results show the mean and S.E.M. of three independent experiments. To verify protein knockdown, cells were harvested 24 h after transfection and the cFLIP levels were assessed in immunoblots. b. BT-474 cells overexpressing Cflipl (pBABE-FLIPL) or control cells (pBABE) were treated with SAHA for 8 h at the doses indicated. After this time, TRAIL (500 ng/ml) was added to some cultures for 15 h. Apoptosis was measured as the percentage of cells with subG1 DNA content and the results show the mean and range of two independent experiments. Western blots show the cFLIP levels after SAHA treatment in pBABE and pBABE-FLIPL cells.

    Invest New Drugs 2012 30, 541-547. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Vorinostat inhibits the tumor growth. NOD ⁄ SCID-B2m-/-mice were injected s.c. with 1×106 MyLa2059 cells into both flanks. When the mice had developed established palpable tumors treatment was initiated (day 1). The mice received either vehicle (black line) or 60 mg ⁄ kg vorinostat (grey line) i.p. five days a week. In total, there were four mice in the group receiving vehicle and three mice in the group receiving vorinostat. The length and width of the tumors were measured continuously until the experiment was terminated on day 20 post treatment initiation. The tumor volume was calculated using the formula V = (a×b2)/2, where a defines the length (mm) and b the width (mm) of the tumor. The histogram shows the average tumor volume per flank ± SEM. * denotes a significant difference (P < 0.05) in the average tumor volume per flank between mice treated with vehicle and vorinostat at the given time-point both by using a one-tailed two samples test with Welch, scorrection and a Mann-Whitney U test.

     

     

    Exp Dermatol 2010 19, 1096-1102. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • (a) Western blot of whole cell extracts demonstrates SAHA and NaB treatment result in increased levels of acetylated histone H3 compared to vehicle controls. (b) Light micrographs of HDACi/vehicle treated cultures illustrate the paucity of neurospheres in HDACi-treated cultures. (c) Quantitative analysis confirms SAHA and NaB inhibit the formation of neurospheres (diameter ≥ 50 μm) and increase the prevalence of small cell clusters (≥ 4 cell, diameter < 50 μm). (d) Flow cytometry using LIVE/DEAD cell stain confirms SAHA and NaB are minimally toxic compared to vehicle controls (n = 3). Scale bar = 100 μm.

    BMC Neurosci 2011 12, 50. Vorinostat (SAHA, MK0683) purchased from Selleck.

    (a) EdU flow cytometry of live cell-gated adult mouse NSCs demonstrates SAHA (**p < 0.01) and NaB (***p < 0.001) significantly inhibit DNA synthesis. (b) Analysis of DNA content by flow cytometry reveals significant accumulation of adult mouse NSCs in G1phase of the cell cycle following SAHA (*p < 0.05) and NaB (*p < 0.05) treatment. (c) Correspondingly the proportion of cells in G2/M phase is significantly reduced following SAHA (*p < 0.05) and NaB (*p < 0.05) treatment. (d) Fluorescence micrographs of HDACi/vehicle treated adult NSCs pulse-labeled with EdU for flow cytometry. Scale bar = 50 μm. Statistical comparisons performed using one-way ANOVA with post hoc Newman-Keuls multiple comparison tests.

    BMC Neurosci 2011 12, 50. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • (a) Immunofluorescence labeling of NeuN, GFAP and Olig2 cell fate markers reveals reduced expression of GFAP (*p < 0.001) and Olig2 (*p < 0.05) in adult mouse NSCs treated with SAHA for 48 hours under proliferation culture conditions and then induced to differentiate for 14 days in culture (two-way ANOVA with Bonferroni post-tests). (b) Western blot of β-catenin protein in nuclear and cytoplasmic fractions of adult mouse NSCs treated with HDACi/vehicle control under proliferation culture conditions. Gapdh and lamin A/C blots are included as loading controls. (c) SAHA and NaB do not significantly alter nuclear/cytoplasmic levels of β-catenin in adult mouse NSCs. Histogram of β-catenin Western blot signal intensities normalized to loading controls (n = 4).

    BMC Neurosci 2011 12, 50. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Morphological changes and histone H3 phosphorylation of HB cells treated with a combination of VX-680 and SAHA. HUH6 and HepT1 were incubated with VX-680 (6 μM) and SAHA (0.5 μM). Nuclei diameter (a) and cell diameter (b) were determined 72 h later by DAPI staining and microscopy. Data represent mean±SD of the diameters from 20 cells in each experiment. (* Two-way ANOVA, Bonferroni test, p \0.05). c Western blot analysis on HUH6 and HepT1 cells were carried out with an anti-phospho-Histone H3 (Ser 10) antibody (p-H3) 24 h after incubation with VX-680 (10 μM), SAHA (0.2 μM) or a combination of both. Controls were left untreated. Western blot analysis showed a decrease in p–H3 after treatment with VX-680 ( lane 2 ) relative to controls ( lane 1 ) and an increase when SAHA was added ( lane 3 ). For the combination of VX-680 and SAHA (lane 4 ) no p-H3 was detected.

    Pediatr Surg Int 2012 28, 579-89. Vorinostat (SAHA, MK0683) purchased from Selleck.

  • Vorinostat promotes C2C12 differentiation.In the image, the red is the myotube indicating differentiation ,the blue is the nuclei.

     

     

    Dr. Akihiko Yoshimura of Akihiko Yoshimura. Vorinostat (SAHA, MK0683) purchased from Selleck.

    Western blot analysis of Acetyl-H3 and H3. 0-20μM SAHA was added.

     

     

    Dr. Zhang of Tianjin Medical University. Vorinostat (SAHA, MK0683) purchased from Selleck.

Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description Vorinostat (suberoylanilide hydroxamic acid, SAHA) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay.
Targets
HDAC [1]
(Cell-free assay)
~10 nM
In vitro

Vorinostat inhibits the activities of HDAC1 and HDAC3 with IC50 of 10 nM and 20 nM, respectively. Vorinostat also results in a marked hyperacetylation of histone H4. [1] Vorinostat inhibits the growth of three prostate cancer cell lines LNCaP, PC-3 and TSU-Pr1 at micromolar concentrations (2.5-7.5 μM), and induces dose-dependent cell death in LNCaP cells. [2] Vorinostat treatment in MCF-7 cells inhibits cell proliferation at an IC50 of 0.75 μM resulting in the accumulation of cells in the G1 and G2-M phase of the cell cycle. Vorinostat also induces differentiation in the estrogen receptor-negative cell line SKBr-3 and the retinoblastoma-negative cell line MDA-468. [3] Vorinostat treatment at 1 μM for 8 hours or more is sufficient to irreversibly induce apoptosis of human multiple myeloma (MM) cells. The gene expression profiles of Vorinostat treated MM cells are not hallmarked by global transcriptional activation, but by coordinated transcriptional changes of specific functional groups of genes such as cytokine-induced proliferative/survival signaling cascades, oncogenes-tumor suppressor genes, regulators of apoptosis, DNA synthesis-repair and cell cycle, and proteasome-ubiquitin function. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
A20 NHf2ZVFMcW6jc3WgRZN{[Xl? NVO4[3lQOSCq MnPwSG1UVw>? M3n2d4FvfGlvSFTBR|Eh[WO2aY\peJkhf2m2aDDJR|UxKG:oIECuNVEzKM7:TR?= NICwdpcyOTl4MES4PS=>
Friend leukemic cell NXLke3dJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4XxbVUxKM7:TR?= MnvnO|IhcA>? NXnre4NOTE2VTx?= NELneJlKSzVyPUCuPVkh|ryP MYCxNlExQTlzMx?=
K562 M3HYN2tqdmG|ZTDBd5NigQ>? MY[2JIg> MWXEUXNQ M17yS2lvcGmkaYTpc44hd2ZiaIXtZY4hcGm|dH;u[UBl\WGlZYT5cIF{\SBqbXn4eJVz\SCxZjDISGFEOSCjbnSgTGRCSzJrIIfpeIghUUN3MDDv[kAxNjBzIN88US=> NYi5c4R2OTJ2MUmzPFA>
HT1080 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWP3b3U5PzJiaB?= MkLuSG1UVw>? MXLJR|UxRTJwNDFOwG0> NEPoeXIyOjJ5MEG3OS=>
H1299 NVzUW29QU2mwYYPlJGF{e2G7 MU[yNEBucW5? M3nOXmROW09? MkDITY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGhFSUNiZX76fY1mKHerdHigTWM2OCCxZjCwMlE6PCEQvF2= MUmxOFUzOTR{Mh?=
H1299 NFLCUoRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkDEO|IhcA>? NYrMO5RSTE2VTx?= MnHXTWM2OD15LkK0JO69VQ>? MWWxOFUzOTR{Mh?=
HeLa M17MOGtqdmG|ZTDBd5NigQ>? Ml\lN|AhdWmw NGPZfI9KdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6|dDDobZN1d26nIHTlZYNmfHmuYYPlJJdqfGhiSVO1NEBw\iByLkK4JO69VQ>? M{PoPVE2PjB|OUS5
LOX-IMVI NIXv[3FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3PLe|Q5KGh? NVXxU4hoUUN3ME2xMlMh|ryP NWfSTZpUOTV5MUW0O|A>
NCI-H226 NFnOOoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV:0PEBp MojaTWM2OD1{Lk[g{txO M3XBUlE2PzF3NEew
RXF-631L Mo\1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU[0PEBp NGe3VmNKSzVyPUKg{txO M1Tv[VE2PzF3NEew
SNB-78 NITLOodIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml3uOFghcA>? MWTJR|UxRTF4IN88US=> MkC3NVU4OTV2N{C=
St-4 NX;oZ|NIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M37Fb|Q5KGh? MV3JR|UxRTVwMjFOwG0> MVexOVcyPTR5MB?=
ARP1 Mn;qSpVv[3Srb36gRZN{[Xl? MXuyJO69VQ>? NHLnbHg3KGh? NX;YTZFvUW6mdXP0bY9vKG:oIGCyNXdCTjFiZ3Xu[UBmgHC{ZYPzbY9v NVe5eGhlOTR5M{S4NFY>
CEM MnnET4lv[XOnIFHzd4F6 NVXTXVduOSCq NX[wW4Z1TE2VTx?= MXPJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwM{Og{txO MVKxOlQzODB4NB?=
HCT15 NEDNeWVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7JcmN6PDhiaB?= NGjmWJZIUTVyPUCuPFIh|ryP M{P2SFE3PzJ|MkK3
NCI-H23 NYjGZpVZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{Xj[lQ5KGh? NIOzOZBIUTVyPUCuPVIh|ryP NEK2cIEyPjd{M{KyOy=>
GM15850 NWX4eoZRU2mwYYPlJGF{e2G7 NXvoUXBrOi53IN88US=> NXz2fGNRQTZiaB?= MYjEUXNQ Ml24TY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViSEOgcJl{cW6nIEG0JIFk\XS7bHH0bY9vKG:oIF\YUkBo\W6n MVOxOlkzOTN4Nx?=
GM15850 NYXyfmFWU2mwYYPlJGF{e2G7 NWXVOmtHOi53IN88US=> NE\oeWY6PiCq MofqSG1UVw>? Mn7uTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViSESgcJl{cW6nIEWgZYNmfHmuYYTpc44hd2ZiRmjOJIdmdmV? NVHYZpZ7OTZ7MkGzOlc>
GM15850 NWXDeJpEU2mwYYPlJGF{e2G7 M2XoWFIvPSEQvF2= NW\iSZVvQTZiaB?= M{LNbWROW09? NWroTnJRUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFQhdHm|aX7lJFEzKGGlZYT5cIF1cW:wIH;mJGZZViCpZX7l M{PFU|E3QTJzM{[3
GM15850 NVvrfIxwU2mwYYPlJGF{e2G7 NFjzcmYzNjVizszN NYj4TYU2QTZiaB?= NW\vclFGTE2VTx?= NHnvNopKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIN{BtgXOrbnWgPUBi[2W2eXzheIlwdiCxZjDGXG4h\2WwZR?= MXuxOlkzOTN4Nx?=
GM15850 NYPpU4dQU2mwYYPlJGF{e2G7 Ml3uNk42KM7:TR?= NI\Mfpk6PiCq MV7EUXNQ NGLCVZlKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIOEBtgXOrbnWgPEBi[2W2eXzheIlwdiCxZjDGXG4h\2WwZR?= M{L3WFE3QTJzM{[3
GM15850 MXXLbY5ie2ViQYPzZZk> NUXCfGFzOi53IN88US=> MXK5OkBp MVrEUXNQ NWnVOXRVUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFQhdHm|aX7lJFE3KGGlZYT5cIF1cW:wIH;mJGZZViCpZX7l NVG0ZY1MOTZ7MkGzOlc>
U937 NHLFWHlHfW6ldHnvckBCe3OjeR?= NV[yTFJHPSEQvF2= NHXXbIQzPCCq NHzaSo1FVVOR NITlNZlE\WyuIHP5Z4xmKGG{cnXzeEBjgSCjY3P1cZVt[XSrb36gZZQhWy:JMj;NJJBp[XOn MYmxO|AxPDdzOB?=
H661 M3;6TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVO0PEBp Mo\VTWM2OD1zIN88US=> NITudVcyPzF3N{CwPS=>
U937 NVrM[JY2TnWwY4Tpc44hSXO|YYm= M3LkOVUh|ryP NGDr[I4zPCCq NY[ycVdNTE2VTx?= NWC2b3lNUW6lcnXhd4UhcW5iaHnzeI9v\SCKMzDhZ4V1gWyjdHnvci=> NG\kbYQyPzR2N{e1NC=>
U937 M2\ye2Z2dmO2aX;uJGF{e2G7 NXm3Znh6OSEQvF2= NXy1OJk{OjRiaB?= M2D6VGROW09? M2faeWVn\mWldDDvckBidHCqYTD0eYJ2dGmwIHHj[ZR6dGG2aX;u NYPmdJl6OTd2NEe3OVA>
RAW264.7 MoD3SpVv[3Srb36gRZN{[Xl? NW\veI5EOTBizszN NVrid3VuOjSq MWf3ZZRmeg>? Ml\5TY5pcWKrdHnvckBw\iCOUGOtd5RqdXWuYYTl[EBVVk[jbIDoZUBxem:mdXP0bY9vKHerdHigTWM2OCCxZjCwMlc3KM7:TR?= M2TNT|E4PDd5NUG4
RAW264.7 MkLISpVv[3Srb36gRZN{[Xl? MkH1NVAh|ryP MVeyOIg> NFLUfpV4[XSnch?= MnfsTY5pcWKrdHnvckBw\iCOUGOtd5RqdXWuYYTl[EBPVyCycn;keYN1cW:wIIfpeIghUUN3MDDv[kAyNjh6IN88US=> MXixO|Q4PzVzOB?=
SC9 Mn\US5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnNZVJGPDhiaB?= NHPQV2xKSzVyPUCuOlA3KM7:TR?= MkXYNVc2ODd{MUm=
ST14A NH3ndGxHfW6ldHnvckBCe3OjeR?= NEXJbVc2KG6P MXG3NkBp NHLac|JKdmO{ZXHz[UBqdiCURWPUM25TW0ZibWLORUBmgHC{ZYPzbY9v NIDx[moyPzV4NUm5Ny=>
ST14A NF\EUWpHfW6ldHnvckBCe3OjeR?= M{LTNlUhdk1? MWK3NkBp NIfoSG9KdmO{ZXHz[UBqdiCHMl[xJI1TVkFiZYjwdoV{e2mxbh?= MmrJNVc2PjV7OUO=
PANC1 M1HDTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYrFbGkzOSEQvF2= M1znbWlEPTB;MD60OUDPxE1? NF7NWFEyPzV6OEe0OC=>
PANC1 MmPYSpVv[3Srb36gRZN{[Xl? NVzBOodNOSEQvF2= NVfTRo9lOjRiaB?= MYPJcoNz\WG|ZTDpckBxOjGZQV[xM2NKWDFiZ3Xu[UBmgHC{ZYPzbY9v MlXwNVc2QDh5NES=
PANC1 NUnYfZJzTnWwY4Tpc44hSXO|YYm= NX3Gc|J{OSEQvF2= MmD2NlQhcA>? MmfZTY5kemWjc3WgbY4heDJ5a3nwNUBo\W6nIHX4dJJme3Orb36= MVyxO|U5QDd2NB?=
PT45 MlrrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2jKR2lEPTB;NDFOwG0> MWmxO|U5QDd2NB?=
ACHN NXfoOGxFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\IRoJjOjFiaB?= NXvjZlJNT0l3ME2wMlY2KM7:TR?= Mn3kNVc3PjJ4ME[=
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SKHEP1 NGTqdW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4\K[FczKGh? Mk\PTWM2OD1|LkS2JO69VQ>? NIHYUWszODF2M{e3PC=>
BJ Mnz1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUOxNFAh|ryP NFXhe3M1QCCq NYL4S|BbTE2VTx?= M3jPWWdzd3e2aDDpcohq[mm2aX;uJIF1KDBwMEWgeI8hOTByIIXN NV;5PWxnOjBzNEO4OFA>
MUF MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrjVnM4OTByIN88US=> M2jyT|Q5KGh? MW\EUXNQ MlGwS5Jwf3SqIHnubIljcXSrb36gZZQhOC5yNTD0c{AyODBidV2= NYq1cGROOjBzNEO4OFA>
Caco-2 NELzepJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFPLTVAyODBizszN NXPkbFhHPDiq NH;mXHdFVVOR NITtemRKSzVyPUiuNUDPxE1? NV;McYF2OjBzNEO4OFA>
Caco-2 MXnLbY5ie2ViQYPzZZk> NGTxO2Q2KM7:TR?= NXnhZnVrPCCq MkSzSG1UVw>? NEjBbW9KdmS3Y4Tpc44hd2ZiSFTBR{1u\WSrYYTl[EBpcXO2b37lJIFk\XS7bHH0bY9vKEmFNUCgc4YhOC5yM{eg{txO NF\pTGozODF2M{i0NC=>
Caco-2 NHzrUZNMcW6jc3WgRZN{[Xl? NYT4dYJPOC5|MU[gcm0hfG9iM{GuOkDPxE1? NIT2NWw1KGh? MoTOSG1UVw>? MVvFfEB3cX[xIHnubIljcXSrb36gc4YhcHWvYX6gTGRCSzFid3n0bEBKSzVyIH;mJFAvOTF7IN88US=> MmnuNlAyPDN6NEC=
Caco-2 NFzhbFRMcW6jc3WgRZN{[Xl? NETCcoMxNjNzNjDuUUB1dyB|MT62JO69VQ>? NGnrXow1KGh? MUfEUXNQ MWrFfEB3cX[xIHnubIljcXSrb36gc4YhcHWvYX6gTGRCSzNid3n0bEBKSzVyIH;mJFAvOTR5IN88US=> NWCwZZBPOjBzNEO4OFA>
Caco-2 NVfpeHZ1U2mwYYPlJGF{e2G7 MlzNOk4{OiCwTTD0c{A3OzJizszN NHfs[m41KGh? MXLEUXNQ NV7NVVg{TXhidnn2c{BqdmirYnn0bY9vKG:oIHj1cYFvKEiGQVO1JJdqfGhiSVO1NEBw\iBzMj62JO69VQ>? M4\pVlIxOTR|OESw
Caco-2 MWnLbY5ie2ViQYPzZZk> MV62MlMzKG6PIITvJFY{OiEQvF2= MW[0JIg> NILnVW9FVVOR NUPHfnZNTXhidnn2c{BqdmirYnn0bY9vKG:oIHj1cYFvKEiGQVO3JJdqfGhiSVO1NEBw\iB2MTFOwG0> NX3vNZZ[OjBzNEO4OFA>
Caco-2 MlPDT4lv[XOnIFHzd4F6 NX7iUFc{OC5|MU[gcm0hfG9iM{GuOkDPxE1? NFHyS4M1KGh? MlXtSG1UVw>? NHXifYFGgCC4aY\vJIlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{[= MoDRNlAyPDN6NEC=
HepG2 MVrLbY5ie2ViQYPzZZk> M4H6fFUh|ryP M2\0TlZp M2ewN2ROW09? NUS5[Iw5UW6mdXP0bY9vKG:oIFjERWMudWWmaXH0[YQhcGm|dH;u[UBi[2W2eXzheIlwdg>? MU[yNFE1Ozh2MB?=
HepG2 M3;neGtqdmG|ZTDBd5NigQ>? NXruXYZrPSEQvF2= NF\nelE3cA>? NVT5bpZUTE2VTx?= NE\h[HRKdmS3Y4Tpc44hd2ZiSFTBR{1u\WSrYYTl[EBpcXO2b37lJIh6eGW{YXPleJlt[XSrb36= M{OxTlIxOTR|OESw
HepG2 MUfLbY5ie2ViQYPzZZk> NIHDV4g2KM7:TR?= NXfyeHV3Pmh? NGC0U2JFVVOR MV3JcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDhcJBp[S22dXL1cIlvKGGlZYT5cIF1cW:w MWKyNFE1Ozh2MB?=
HepG2 Mmn5T4lv[XOnIFHzd4F6 NWXtZ2RiPSEQvF2= M1;URlZp NFnCSGJFVVOR MVTJcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDIN{9JPCCqaYP0c45mKGGlZYT5cIF1cW:w Mn3RNlAyPDN6NEC=
COLO205 NUPobndwU2mwYYPlJGF{e2G7 MkT2NVAh|ryP M3HLZVI1KGh? MoHQSG1UVw>? MVPJcohq[mm2aX;uJI9nKEiGQVOxJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDobZN1d26nIFizJIFk\XS7bHH0bY9vKHerdHigTWM2OCCxZjCyMlIh|ryP NI\xXIIzODR3MUO3PC=>
SCC4 MmrET4lv[XOnIFHzd4F6 M4HS[FEh|ryP NEHOOZEzPGh? MkTjTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIIT1ZpVtcW5iYXPleJlt[XSrb36= MYKyNFQ2OjJ{NR?=
SCC4 Ml;nT4lv[XOnIFHzd4F6 NVjG[o1TOSEQvF2= NVXBeog{OjSq MmPtTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViYXPleJlt[XSrb36= NH;VcnUzODR3MkKyOS=>
SCC4 NGC1RVlHfW6ldHnvckBCe3OjeR?= NITtXo0yKM7:TR?= MkfyOUBucW5? NHrxXZBC\2:waYP0JIFkfGm4aYT5JIF1KF[GUjDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZiQ2nQNlQh\2WwZTDlfJBz\XO|aX;u NGO2TVMzODR3MkKyOS=>
NB4 MmjGSpVv[3Srb36gRZN{[Xl? Mn7QOUDPxE1? M1S5O|I1KGh? M1\s[2ROW09? MWHD[YxtKGO7Y3zlJIFzemW|dDDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCjY3P1cZVt[XSrb36gZZQheHKnLVexJJBp[XOn M1u3[|IxPDlzNESw
NB4 NESzelVMcW6jc3WgRZN{[Xl? MnjrOUDPxE1? MX6yOEBp MXrEUXNQ MonQTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHHj[ZR6dGG2ZTD0eYJ2dGmwIHzleoVt MnLBNlA1QTF2NEC=
NB4 NXK3W2l7TnWwY4Tpc44hSXO|YYm= NILxbI02KM7:TR?= M3jobVI1KGh? NVy5SG5KTE2VTx?= NX3DU|VJUW6mdXP0bY9vKG:oIICyNXdCTjFxQ1nQNUBmgHC{ZYPzbY9v MVeyNFQ6OTR2MB?=
NB4 NEXZNFJMcW6jc3WgRZN{[Xl? M4[5fVUh|ryP NF;BdFczPCCq M1r2c2ROW09? Mm\HTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViSEOgZYNmfHmuYYTpc44> NIPvTXkzODR7MUS0NC=>
NUGC3 NGfnOGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmnPS2k2OD1{Lkm0JO69VQ>? NY\BRnQzOjB6NUC5O|E>
A549 NUjyZ|dkTnWwY4Tpc44hSXO|YYm= M1jEW|Uh|ryP NEnSSVE{KGh? M3nUdGROW09? M2n2eGlv\HWldHnvckBw\iCjdYTvdIhi\3liaX6gbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCOQ{PJTS=> Mny5NlA5PTV{MEi=
HUT78 M1P1Smdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NILzbFY4OiCq MXrEUXNQ Mn3qS2k2OD1yLkOg{txO NFLM[IUzODh6NEKwPC=>
U937 NW\1W5B6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\DPWlZPzJiaB?= M3L4d2ROW09? NYPsSYFQT0l3ME2wMlM6KM7:TR?= M{OweVIxQDh2MkC4
A431 MX\DfZRwfG:6aXOgRZN{[Xl? MkTaO|IhcA>? MX7DfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDBOFMyKGOnbHzzJI93\XKneIDy[ZN{cW6pIFXHSnIhf2m2aDDJR|UxKG:oIESuNkDPxE1? MUiyNVA5ODZ{OR?=
A549 M4PzRWN6fG:2b4jpZ{BCe3OjeR?= MYG3NkBp Mn7pR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{BwfmW{ZYjwdoV{e2mwZzDISGFEKHerdHigTWM2OCCxZjCxMlgh|ryP MoH3NlExQDB4Mkm=
CAL27 MnG2R5l1d3SxeHnjJGF{e2G7 MXe3NkBp M{DoOmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGNCVDJ5IHPlcIx{KG:4ZYLlfJBz\XO|aX7nJGVITlJid3n0bEBKSzVyIH;mJFMvOiEQvF2= M4PSV|IyODhyNkK5
HeLa NWjwVHMzS3m2b4TvfIlkKEG|c3H5 MnnyO|IhcA>? NH3zbpNEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJ\UyjIHPlcIx{KG:4ZYLlfJBz\XO|aX7nJGhFSUNid3n0bEBKSzVyIH;mJFEvPSEQvF2= NEXlfnYzOTB6ME[yPS=>
Sf21 MXPLbY5ie2ViQYPzZZk> NVqzRZllOyCq NX:5PVVPUW6qaXLpeIlwdiCxZjDGcIFoKHSjZ3fl[EBpfW2jbjDy[YNwdWKrbnHueEBJTEGFODDlfJBz\XO|ZXSge4l1cCCLQ{WwJI9nKDFwMjFOwG0> NVnufmtbOjFyOEC2Nlk>
SKBR3 MVTDfZRwfG:6aXOgRZN{[Xl? MmrsO|IhcA>? MlqzR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2tDWjNiY3XscJMhd3[ncnX4dJJme3OrbnegTGVTOiC5aYToJGlEPTBib3[gNk43KM7:TR?= MlXFNlExQDB4Mkm=
SKOV3 MonHR5l1d3SxeHnjJGF{e2G7 NYL5XpBXPzJiaB?= NXnES3lKS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hW0uRVkOgZ4VtdHNib4\ldoV5eHKnc4PpcochUEWUMjD3bZRpKEmFNUCgc4YhOi5{IN88US=> M2jw[lIyODhyNkK5
COR-L23 NI\ufYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXOxNEDPxE1? Mnm1O|IhcA>? NVL3V3FpTE2VTx?= MoDiS2k2OD1zLkGg{txO NUXlcJNlOjFyOEC2OFc>
DU145 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlO3NVAh|ryP M2rL[|czKGh? NGDNcYhFVVOR M4\1WmdKPTB;MT6wOUDPxE1? NEH0bJgzOTB6ME[0Oy=>
LoVo MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF3KTHEyOCEQvF2= M4HsOVczKGh? NXPxXXRQTE2VTx?= MXvHTVUxRTFwNzFOwG0> M4\kN|IyODhyNkS3
MCR5-SV2 MmL2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWC4WlZTOTBizszN NFW0emk4OiCq NUL3TXh2TE2VTx?= MWnHTVUxRTJwMTFOwG0> NX63SVNZOjFyOEC2OFc>
HCT116 MXnLbY5ie2ViQYPzZZk> M3XPVVMxKM7:TR?= MX6zNEBp NWH3UFgyTE2VTx?= MnTGTY5pcWKrdHnvckBw\iCKRFHDOkBie3Onc4Pl[EBieyCrbnT1Z5Rqd25ib3[gZYxxcGFvdIXieYxqdiCjY3X0fYxifGmxbh?= NF3mNFgzOTB6ME[0Oy=>
HCT116 NV7M[YdxU2mwYYPlJGF{e2G7 MYSzNEDPxE1? MmGxN|AhcA>? M1fOO2ROW09? MVrJcohq[mm2aX;uJI9nKEiGQVOxJIF{e2W|c3XkJIF{KGmwZIXjeIlwdiCxZjDobZN1d26nIFizT|kh[WOndInsZZRqd25? MlrVNlExQDB4NEe=
U937 M3;oOmtqdmG|ZTDBd5NigQ>? MnvHOUDPxE1? MniyNlQhcA>? NV7vR5N{TE2VTx?= MULJcohq[mm2aX;uJI9nKGirc4TvcoUh[WOndInseJJidnOoZYLhd4Uh[XO|ZYPz[YQh[XNiaX7jdoVie2Vib3[gZYNmfHmuYYTpc44hd2ZiaHnzeI9v\SCKMzDheEBMQQ>? MW[yNVI6OjR7Mh?=
U937 MkfpT4lv[XOnIFHzd4F6 MlXsOUDPxE1? MoTNNlQhcA>? M2\4eGROW09? NIq0PY9KdmirYnn0bY9vKG:oIHjpd5RwdmViYXPleJltfHKjboPm[ZJie2ViYYPz[ZN{\WRiYYOgbY5kemWjc3Wgc4Yh[WOndInsZZRqd25ib3[gbIl{fG:wZTDIN{BifCCNMUi= MXmyNVI6OjR7Mh?=
U937 M1jn[WtqdmG|ZTDBd5NigQ>? MmXKOUDPxE1? MYOyOEBp Mkf1SG1UVw>? Mn\vTY5pcWKrdHnvckBw\iCqaYP0c45mKGGlZYT5cJRz[W6|ZnXyZZNmKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIH;mJJBidi2jY3X0fYxifGWmIHjpd5RwdmViSESgcIV3\Wx? MXGyNVI6OjR7Mh?=
U937 MlrRT4lv[XOnIFHzd4F6 M1\5SlUh|ryP NGXaV24yPSCvaX6= MVLEUXNQ M1n2cmlvcGmkaYTpc44hd2ZiaHnzeI9v\SCjY3X0fYx1emGwc3\ldoF{\SCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDv[kBpcXO2b37lJGg{KGGlZYT5cIF1cW:wIHzleoVt MVmyNVI6OjR7Mh?=
A2780 MkS3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1rSWlk3KGh? M17mcGROW09? Ml;GTWM2OD1zLk[yJO69VQ>? MoDlNlE3OzR2M{C=
COLO205 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWe5OkBp NWi2PVZLTE2VTx?= NHm3b41KSzVyPUKuNVIh|ryP MYiyNVY{PDR|MB?=
Hep3B MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2GzRlQ5KGh? NWD2VWw1TE2VTx?= MlTES2k2OD1zLkKxJO69VQ>? MX6yNVcyOjF2Nh?=
GM11272 NHfYblBHfW6ldHnvckBCe3OjeR?= NFHuTJIyODBibl2= NVqzZmt5PzJiaB?= NF\vS3dFVVOR NF\6[oRT\WGldHn2ZZRqd25ib3[gUYVEeDJibYX0ZY51KGW6cILld5Nqd25iYYSgNVAhfG9iMUCwJI5O M1fjZVIyQDRyN{G2
CFBE41o MkPFT4lv[XOnIFHzd4F6 NVPLe2V{PSEQvF2= NFz3WVdFVVOR Mk[4SI94diC{ZXf1cIF1cW:wIH;mJGhFSUN5IHH0JFAvOiC2bzC1JJVO MVWyNVk5PDl3OB?=
CFBE41o MlvoSpVv[3Srb36gRZN{[Xl? NHrnclcyKM7:TR?= M4DNO|I1KGh? NYK3TpdJTE2VTx?= MWfS[ZN1d3KjdHnvckBw\iCFRmTSJIRmdHSjRkWwPEBufXSjboSgeJJi\m[rY3vpcochfG9iY3XscEB{fXKoYXPlJIF{KGeueXPv[o9zdXN? MoHRNlE6QDR7NUi=
DU145 NXLZdYdnU2mwYYPlJGF{e2G7 NVPiSHFZOi53IN88US=> NES4VnQzPCCq M1nVcGROW09? MYrJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhfXC{ZXf1cIF1cW:wIH;mJJAzOXejZkGgdJJwfGWrbjDlfJBz\XO|aX;u Mkm0NlIzPjBzNk[=
DU145 MkfET4lv[XOnIFHzd4F6 MUC1JO69VQ>? NH\WfIQzPCCq MUDEUXNQ NV;jO5RGUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHjpd5RwdmViSESgZYNmfHmuYYTpc44> M{XFT|IzOjZyMU[2
DU145 NUP4XJR[U2mwYYPlJGF{e2G7 M375PFUh|ryP NWK2RVdXOjRiaB?= NUjje5V7TE2VTx?= NUHB[mNmUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHjpd5RwdmViSEOgbJlx\XKjY3X0fYxifGmxbh?= Mnq0NlIzPjBzNk[=
786-0 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLBbGI3NjJizszN MoHhO|IhcA>? NX36Vng5TE2VTx?= NFLDbGxIUTVyPUOuNkDPxE1? NEHlVI4zOjR|NU[2PS=>
A498 NGfuSW1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlXDOk4zKM7:TR?= M{C1XFczKGh? MlrtSG1UVw>? MmfyS2k2OD1zLk[g{txO MorXNlI1OzV4Nkm=
A549/ATCC NHX5dFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NULzcIVJPi5{IN88US=> MWS3NkBp NYL4XGUxTE2VTx?= NFrHPZhIUTVyPUGuOkDPxE1? MXeyNlQ{PTZ4OR?=
ACHN MmjIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYS2MlIh|ryP NYfMOGRUPzJiaB?= M2KwTmROW09? NFzsRlZIUTVyPUGuN{DPxE1? NYLCXIVyOjJ2M{W2Olk>
BT549 M3LLOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmSxOk4zKM7:TR?= MVi3NkBp M4raPGROW09? NVzBe5VFT0l3ME2xMlMh|ryP MVeyNlQ{PTZ4OR?=
EKVX NVSxd4RGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXn1U5NuPi5{IN88US=> NX;LU|VbPzJiaB?= MVvEUXNQ M3TXS2dKPTB;MT6zJO69VQ>? Mmi4NlI1OzV4Nkm=
HCT15 Mnz3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYi2MlIh|ryP NX\RZ3hPPzJiaB?= M3uzWGROW09? M3fnO2dKPTB;Mj61JO69VQ>? Mn\3NlI1OzV4Nkm=
HOP92 M3PIZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEHCdmY3NjJizszN MYW3NkBp NX:yOGI5TE2VTx?= M1f5cGdKPTB;NDFOwG0> NWfsO|A2OjJ2M{W2Olk>
IGROV1 M4CzdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml\2Ok4zKM7:TR?= M1rtTlczKGh? Mki3SG1UVw>? MkTLS2k2OD1zLkOg{txO MknlNlI1OzV4Nkm=
LOXIMVI NXPuVFBET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYfaZmdnPi5{IN88US=> NFPjVFM4OiCq MnfPSG1UVw>? MmjuS2k2OD1zIN88US=> NVvvXItEOjJ2M{W2Olk>
M14 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnz3Ok4zKM7:TR?= NWSySm1sPzJiaB?= M4nZR2ROW09? MX7HTVUxRTFwMzFOwG0> Mlv5NlI1OzV4Nkm=
MALME-3M NVK5W4hLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{O0TlYvOiEQvF2= M33MZ|czKGh? MkPVSG1UVw>? NYfifpJzT0l3ME2wMlMzKM7:TR?= NEHu[nczOjR|NU[2PS=>
MCF10A MkfyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWe2MlIh|ryP MXy0PEBp MmPlSG1UVw>? MYnHTVUxRTlwNjFOwG0> NWLudIt6OjJ2M{W2Olk>
MDA-MB-435 NFzzXHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkL0Ok4zKM7:TR?= NXHqbYV4PzJiaB?= NITHU5pFVVOR NYjYSZNRT0l3ME2wMlUh|ryP MmPUNlI1OzV4Nkm=
MDA-MB-468 MkK1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2KycFYvOiEQvF2= MnzYO|IhcA>? M2KyS2ROW09? MXnHTVUxRTBwNkOg{txO NE[4bJgzOjR|NU[2PS=>
MOLT4 MoXCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWq2MlIh|ryP NWPF[VV4PzJiaB?= MXvEUXNQ M4izXWdKPTB;MD60JO69VQ>? NFf3[lYzOjR|NU[2PS=>
NCI-ADR-RES MXrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2X3[FYvOiEQvF2= M4jHXFczKGh? MlXFSG1UVw>? MXfHTVUxRTBwMU[g{txO MnjCNlI1OzV4Nkm=
NCI-H23 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjVOk4zKM7:TR?= M3TrZVczKGh? MoHMSG1UVw>? M1\VN2dKPTB;MTFOwG0> NEfI[WszOjR|NU[2PS=>
NCI-H322M MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1HOdVYvOiEQvF2= MmOwO|IhcA>? NHG5[IpFVVOR M1HiTGdKPTB;MD63PUDPxE1? MkToNlI1OzV4Nkm=
NCI-H460 NIqxcIZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV32XGZ2Pi5{IN88US=> NFTOPWc4OiCq NG\XOIJFVVOR M1fDeGdKPTB;MD63PUDPxE1? NXPofnpqOjJ2M{W2Olk>
NCI-H522 MkPOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{DHTFYvOiEQvF2= MnjHO|IhcA>? MkHJSG1UVw>? MVPHTVUxRTBwNTFOwG0> NX;u[IxtOjJ2M{W2Olk>
OVCAR3 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWS2MlIh|ryP M1;pNlczKGh? NEjZNVNFVVOR MkTSS2k2OD1zLkOg{txO MnPDNlI1OzV4Nkm=
OVCAR4 MlPFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2qzVFYvOiEQvF2= M33mbVczKGh? M3KwPGROW09? M4jQOWdKPTB;NDFOwG0> MoO3NlI1OzV4Nkm=
OVCAR5 NFniOGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX22MlIh|ryP NVvxR|VsPzJiaB?= NGjweGNFVVOR Mnr4S2k2OD1yLke5JO69VQ>? MXKyNlQ{PTZ4OR?=
OVCAR8 M{D4PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFGyZYk3NjJizszN MkTMO|IhcA>? M4X3XWROW09? MYjHTVUxRTBwNTFOwG0> NXHFSJU6OjJ2M{W2Olk>
RXF393 NWTxfpBjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3XXUFYvOiEQvF2= MWm3NkBp NGr3OpBFVVOR M{LLVWdKPTB;MT6zJO69VQ>? MlfFNlI1OzV4Nkm=
SF268 MlfrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXn6VoRxPi5{IN88US=> NUH6OmdOPzJiaB?= MnPOSG1UVw>? MlnsS2k2OD1zLk[g{txO M4\SflIzPDN3Nk[5
SF295 M2nVeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M33pcFYvOiEQvF2= NF30fHQ4OiCq NX;pOVQ1TE2VTx?= MlT1S2k2OD1zLk[g{txO MVGyNlQ{PTZ4OR?=
SF539 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIjhPYI3NjJizszN MlvZO|IhcA>? NEDnfZNFVVOR NGXSboFIUTVyPUKg{txO Mnu4NlI1OzV4Nkm=
SK-MEL-2 Mo[zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{\3b|YvOiEQvF2= M3njPFczKGh? NF7abnZFVVOR MUfHTVUxRTFwMzFOwG0> NXzBXXlyOjJ2M{W2Olk>
SK-MEL-28 MnrhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXS1bJVjPi5{IN88US=> MXu3NkBp M3GzcmROW09? NHfVPIhIUTVyPUGg{txO NG\ke2MzOjR|NU[2PS=>
SK-MEL-5 NHTkS2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXnCd|VYPi5{IN88US=> NYHTeopjPzJiaB?= MnvBSG1UVw>? M1K3TGdKPTB;MD62N{DPxE1? NYPKc2toOjJ2M{W2Olk>
SKOV3 MnrHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYK2MlIh|ryP MV63NkBp NFvoUWlFVVOR M4LtXWdKPTB;MTFOwG0> MUCyNlQ{PTZ4OR?=
SN12C MlPhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmTKOk4zKM7:TR?= NIrmdVA4OiCq M2nKe2ROW09? MW\HTVUxRTJizszN M4fG[|IzPDN3Nk[5
SNB19 NFr5O5VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{jjNFYvOiEQvF2= M4rySFczKGh? NF2xenVFVVOR NFLWW2xIUTVyPUCuOlMh|ryP MXGyNlQ{PTZ4OR?=
SNB75 M{Xyc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLBOk4zKM7:TR?= M3nscFczKGh? M{PhPGROW09? NWC1fXA2T0l3ME2wMlc6KM7:TR?= MXWyNlQ{PTZ4OR?=
SR M2Xrdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn6wOk4zKM7:TR?= Mlr1O|IhcA>? NYLVWndrTE2VTx?= MlH4S2k2OD1yLkSg{txO NELhfnUzOjR|NU[2PS=>
T47D NWjO[GRrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2r2cVYvOiEQvF2= NEnYR444OiCq Mn22SG1UVw>? NIDRO3lIUTVyPUCuOUDPxE1? NFTQeYUzOjR|NU[2PS=>
TK10 MoryS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3e1eFYvOiEQvF2= NV:4cFdGPzJiaB?= NUjOeGQ4TE2VTx?= M{n4WmdKPTB;MD62N{DPxE1? MlmzNlI1OzV4Nkm=
UACC257 Mln2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1yz[FYvOiEQvF2= NVnOTZE2PzJiaB?= NXG3NHl1TE2VTx?= Mkm1S2k2OD1yLkWg{txO M3\VVVIzPDN3Nk[5
UACC62 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHXTcZc3NjJizszN NH3oWnU4OiCq NWC0fY9YTE2VTx?= NED0OGNIUTVyPUCuOEDPxE1? NGXUbW8zOjR|NU[2PS=>
UO31 MofFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEHmXIQ3NjJizszN NHrJRXE4OiCq M{jyTWROW09? MonmS2k2OD1yLkWg{txO M1r1flIzPDN3Nk[5
Hep3B Ml\PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mln5OFghcA>? MlLNSG1UVw>? NF\QRYJIUTVyPUCuOlkh|ryP M1jUTVIzPDN7OE[z
BE(2)-C NVvYR2J{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWe3NkBp NHjBU3hFVVOR MkmzTWM4OD1{IN88US=> NILoUXkzOjl|MkOxOi=>
Bel7402 M1fjVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYTCdIhHPzJiaB?= Mo\QSG1UVw>? NIjGeHhIUTlyPUG3JO69VQ>? MWSyN|A3OTN5Nh?=
Bel7404 MVXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWXwXYZjPzJiaB?= MmfOSG1UVw>? M37nfWdKQTB;MUig{txO NILod48zOzB4MUO3Oi=>
BGC823 NGTqOJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH3NT2w4OiCq NG\ydGJFVVOR MlTLS2k6OD15LkWg{txO MVmyN|A3OTN5Nh?=
MGC803 NHHJNZdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXpcGNOPzJiaB?= M4fETmROW09? MVnHTVkxRTFyLkig{txO MVGyN|A3OTN5Nh?=
SGC7901 NHTIb5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFXNU2k4OiCq M2PhW2ROW09? NHv3fZFIUTlyPUmuOEDPxE1? M{PUZ|I{ODZzM{e2
SKHEP1 NFnobVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFjpbIs4OiCq NVzsfYFoTE2VTx?= Moj2S2k6OD1zNzFOwG0> NG\0[VczOzB4MUO3Oi=>
SMMC7721 M3KyOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH24[3A4OiCq Mn[2SG1UVw>? MVrHTVkxRTJyIN88US=> MVuyN|A3OTN5Nh?=
NUGC3 MmjPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXuxNEDPxE1? NEDSOGpIUT1{Lkm0JO69VQ>? M4H0eFI{OTZ|M{Oy
A2780 CisR MUnLbY5ie2ViQYPzZZk> M3rkPVE5KGh? MW\Jcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNESg{txO NF;hOWUzOzJ3Mk[wNy=>
CAL27 sens MWrLbY5ie2ViQYPzZZk> NFLKPVMyQCCq MU\Jcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwM{[g{txO NVvEXmNXOjN{NUK2NFM>
CAL27 CisR NELEOmRMcW6jc3WgRZN{[Xl? M{fOUlE5KGh? M1;YW2lvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD62NUDPxE1? M1LOdlI{OjV{NkCz
KYSE-510 CisR NEX3N3hMcW6jc3WgRZN{[Xl? MYexPEBp MVHJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNUmg{txO NHLLVmQzOzJ3Mk[wNy=>
KYSE-510 sens M{DQbmtqdmG|ZTDBd5NigQ>? MmH3NVghcA>? NX7kWlVNUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlch|ryP NFPtdVAzOzJ3Mk[wNy=>
MDA-MB-231 sens MnrMT4lv[XOnIFHzd4F6 M{[xVFE5KGh? NWDhd|RvUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlYyKM7:TR?= MWCyN|I2OjZyMx?=
MDA-MB-231 CisR NYDVcnk4U2mwYYPlJGF{e2G7 MV[xPEBp MofMTY5pcWKrdHnvckBw\iCKRFHDJJdqfGhiSVO1NEBw\iByLk[xJO69VQ>? MYmyN|I2OjZyMx?=
ES2 M4Hs[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{n6W|Q5KGh? NU\MSIhNTE2VTx?= NYCwZmY4UUN3ME2xNk44KM7:TR?= NGPtPGQzOzR7M{S0PS=>
MDA-MB-231 NXLPdG84U2mwYYPlJGF{e2G7 M2GyZ|ExKM7:TR?= MXqzNEBucW5? NETN[GxFVVOR NFzVcHBKdmirYnn0bY9vKG:oIFjERWMz[Q>? NHj1dG0zOzR7M{S0PS=>
MDA-MB-231 NWe3d3o4U2mwYYPlJGF{e2G7 M2f1SFEh|ryP NF7IS2szPCCq MX\EUXNQ NV;3PHBDUW6qaXLpeIlwdiCxZjDISGFEPiCjc4Pld5Nm\CCjczDhZ4V1gWyjdHnvckBt\X[nbIOgc4YhfHWkdXzpci=> NX\RZYs1OjN2OUO0OFk>
HS68 MYHDfZRwfG:6aXOgRZN{[Xl? Mn21NVAh|ryP MlHuO|IhcA>? MVHEUXNQ MoHBTWM2OD12Lk[g{txO M1jFXlI{PTdyNUSy
MEF M3z2SGN6fG:2b4jpZ{BCe3OjeR?= M1zKXVExKM7:TR?= MWW3NkBp NYTKdopxTE2VTx?= NUL1bGF1UUN3ME20MlQh|ryP MorFNlM2PzB3NEK=
BGC823 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFLoZVJFVVOR MnPpS2k2OD1|LkC5JO69VQ>? MXSyN|YxOTdyNh?=
High5 M3\SfmtqdmG|ZTDBd5NigQ>? M3;YXFI1KGh? MmnoTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDOkB4cXSqIFnDOVAhd2ZiMD6wPUDPxE1? MlfCNlM3ODJ3MkO=
High5 Mlf3T4lv[XOnIFHzd4F6 Ml3nNlQhcA>? M2jkd2lvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiSFTBR|Ehf2m2aDDJR|UxKG:oIECuNVIh|ryP MXOyN|YxOjV{Mx?=
High5 NGTXWG9McW6jc3WgRZN{[Xl? MYKyOEBp NYjQNG0xUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEOyC5aYToJGlEPTBib3[gNE4yPyEQvF2= MVWyN|YxOjV{Mx?=
A549 NEfrcmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXfRXXM6TE2VTx?= Mm\CTWM2OD1zLkC3PUDPxE1? MXqyN|Y1PDJzMB?=
HCT116 M4n3cWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;LSG1UVw>? MXHJR|UxRTBwN{W3JO69VQ>? NHXHbIEzOzZ2NEKxNC=>
K562 NIfiTYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGPjdppFVVOR NEPoW4tKSzVyPUCuOlQ2KM7:TR?= NEL5[4kzOzZ2NEKxNC=>
GBC-SD MoPwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH32TJM2KM7:TR?= MkXIO|IhcA>? NH3IZ2pFVVOR NHW5dpNKSzVyPUG0Mlgh|ryP NEXtS28zOzdyN{K2NC=>
HGC27 Ml;TS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUW1JO69VQ>? NF;G[JM4OiCq MV\EUXNQ NVHOVW9SUUN3ME21MlQh|ryP Mm\QNlM4ODd{NkC=
HL60 MkTRT4lv[XOnIFHzd4F6 NFe5W4I2KM7:TR?= NUnHOG06OjRiaB?= M2nZXmROW09? MYPJcohq[mm2aX;uJI9nKEiGQVO= M4HnOVI{PzB5Mk[w
Vero NH6xW2pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFHzZ2o4OiCq NH3DRnFFVVOR NF:2WFhKSzVyPUCuPUDPxE1? M3fiPVI{Pzh4NEWy
NCI60 NFzH[HhEgXSxdH;4bYMhSXO|YYm= M1G2bVQ5KGh? NYPHXoV3TE2VTx?= NGrMTHhIUT1yLkWzJO69VQ>? MoP3NlQxOTV|Mke=
SW620 NGfNRmpEgXSxdH;4bYMhSXO|YYm= Mn;nOFghcA>? MljsSG1UVw>? M1i1bmdKRTBwNUSg{txO MkDQNlQxOTV|Mke=
U251 NHX0SWpEgXSxdH;4bYMhSXO|YYm= NEnkXnM1QCCq Mn:3SG1UVw>? NXrvRoFVT0l;MT61N{DPxE1? NF\TTGszPDBzNUOyOy=>
KM12 NVrxenhRS3m2b4TvfIlkKEG|c3H5 M1vPRlQ5KGh? MXLEUXNQ MkH0S2k:OS56ODFOwG0> Mn72NlQxOTV|Mke=
Hs578T NYn3UWhPS3m2b4TvfIlkKEG|c3H5 NI\3Z201QCCq MkLESG1UVw>? M2rnW2dKRTRwOEOg{txO NEPNOlAzPDBzNUOyOy=>
MDA-MB-231 NXjtc5hnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYT4R3B7PDhiaB?= MVfJR|UxRTBwMU[g{txO NGPl[m0zPDB7NUCxOi=>
MCF7 M4XPc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1v1UFQ5KGh? NILSc2hIUT1yLkS1JO69VQ>? NVfve3ZiOjRzMUm1OVU>
MDA-MB-435 NGroN29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3j3cFQ5KGh? NVznflhQT0l;MD64PEDPxE1? NH:zWGszPDFzOUW1OS=>
AsPC1 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFfPWFc{OCEQvF2= NX;iSVlFPDhiaB?= NH7MdHBFVVOR NHLRSohKSzVyPUOuOlYh|ryP NUXaTJZQOjRzOEWzO|g>
Jurkat E6.1 MYPLbY5ie2ViQYPzZZk> Mnq3NkBp MoC3SG1UVw>? M1nFWWlvcGmkaYTpc44hd2ZiSFTBR|Qhf2m2aDDJR|UxKG:oIECuNFQh|ryP MVyyOFI3OTh4Mh?=
KG1 NUPCRlRHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mn7GOFghcA>? NIC2R4FKSzVyPUGuNVUh|ryP NETmXZEzPDV{NUCwNy=>
HEL MoXiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnL0OFghcA>? MmC2SG1UVw>? MYHJR|UxRTBwNEmg{txO M4L2NFI1Pjl2MEW1
HSC3 MUXDfZRwfG:6aXOgRZN{[Xl? NILoXIgyOiCq NH7IV3dFVVOR MX;JR|UxRTBwN{Gg{txO NYrkTppzOjR5Nk[1OlA>
HT-29 NHL0c5lEgXSxdH;4bYMhSXO|YYm= NH;VOFUyOiCq M{DqOmROW09? M3\UOmlEPTB;MD63NkDPxE1? NWCzdlBxOjR5Nk[1OlA>
KB NIDMR4hEgXSxdH;4bYMhSXO|YYm= NIDSfIIyOiCq Mli0SG1UVw>? NIjoU3lKSzVyPUCuO|Uh|ryP NFPCb2wzPDd4NkW2NC=>
H460 MlmyR5l1d3SxeHnjJGF{e2G7 MYmxNkBp M33VSGROW09? MWTJR|UxRTBwOEKg{txO MXGyOFc3PjV4MB?=
MONE1 MkG4R5l1d3SxeHnjJGF{e2G7 MmLzNVIhcA>? NYK1RWdsTE2VTx?= MYTJR|UxRTFwMUig{txO M4rlT|I1PzZ4NU[w
MKN45 NYXNbY16S3m2b4TvfIlkKEG|c3H5 NGq5fIEyOiCq MYjEUXNQ MlS4TWM2OD1zLkK1JO69VQ>? MVKyOFc3PjV4MB?=
TSGH M2fWZmN6fG:2b4jpZ{BCe3OjeR?= MlfXNVIhcA>? M3LKXGROW09? Mn;rTWM2OD1{LkC5JO69VQ>? MlS0NlQ4PjZ3NkC=
HL60 MorwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2nkfVczKGh? M1HYU2ROW09? MWDHTVUxRTBwOUOg{txO Ml3oNlQ6ODB3MEC=
HEK293 MnfhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWflS5l{PzJiaB?= NGnnfGtFVVOR NFG1OZBIUTVyPUGzJO69VQ>? NVTGR2lMOjR7MEC1NFA>
Jurkat MmriR5l1d3SxeHnjJGF{e2G7 NWLaNFZyOTByIN88US=> MYG3NkBp M134cmdKPTB;MD65JO69VQ>? NEPCRoQzPDlyMEe0Ny=>
GM15850 MmrlT4lv[XOnIFHzd4F6 Mk[4Nk42KM7:TR?= M2iyR|k3KGh? NV;Hc4JjTE2VTx?= Mlr1[I9meyCwb4SgbY5pcWKrdDDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFMhdHm|aX7lJFE1KGGlZYT5cIF1cW:wIH;mJGZZViCpZX7l NUTPUpliOTZ7MkGzOlc>
GM15850 NETIXGNMcW6jc3WgRZN{[Xl? M1TIeVIvPSEQvF2= MmTvPVYhcA>? NFrUc|dFVVOR MYHkc4V{KG6xdDDpcohq[mm2IFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIOEBtgXOrbnWgOUBi[2W2eXzheIlwdiCxZjDGXG4h\2WwZR?= NFfQVHkyPjl{MUO2Oy=>
GM15850 MWnLbY5ie2ViQYPzZZk> MUKyMlUh|ryP MWC5OkBp MWDEUXNQ M2q2ZoRw\XNibn;0JIlvcGmkaYSgTGRCSyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBpcXO2b37lJGg1KGy7c3nu[UAyOiCjY3X0fYxifGmxbjDv[kBHYE5iZ3Xu[S=> NVLFVFBrOTZ7MkGzOlc>
GM15850 MkXpT4lv[XOnIFHzd4F6 MWCyMlUh|ryP MlruPVYhcA>? NXz4VFVzTE2VTx?= Mn\3[I9meyCwb4SgbY5pcWKrdDDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFMhdHm|aX7lJFkh[WOndInsZZRqd25ib3[gSnhPKGenbnW= MnXGNVY6OjF|Nke=
GM15850 M4jVN2tqdmG|ZTDBd5NigQ>? NF74eZkzNjVizszN MVK5OkBp MlHTSG1UVw>? NX6xe|Fx\G:nczDuc5QhcW6qaXLpeEBJTEGFIHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKGirc4TvcoUhUDRibInzbY5mKDhiYXPleJlt[XSrb36gc4YhTliQIHflcoU> MXGxOlkzOTN4Nx?=
GM15850 MYLLbY5ie2ViQYPzZZk> NH7LT4szNjVizszN MVK5OkBp NGj2VGhFVVOR M17EbYRw\XNibn;0JIlvcGmkaYSgTGRCSyCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDpckBpcXO2b37lJGg1KGy7c3nu[UAyPiCjY3X0fYxifGmxbjDv[kBHYE5iZ3Xu[S=> MWOxOlkzOTN4Nx?=
GM15850 Ml\OSpVv[3Srb36gRZN{[Xl? M3XqS|Uh|ryP M{DpVlEzKGh? MWDEUXNQ M1r4[4hieyCwbzDl[oZm[3Rib36gSnhPKG2UTlGg[ZhxemW|c3nvci=> MX6xOlkzOTN4Nx?=
U937 M{f3bmtqdmG|ZTDBd5NigQ>? M4rtSFUh|ryP M33aOFI1KGh? MYDEUXNQ MUjoZZMhdm9iZX\m[YN1KG:wIHHsdIhiKHS3YoXsbY4h[WOndInsZZRqd25? Mn;sNVc1PDd5NUC=
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NKM-1 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV;JR|UxRTV2ND6yO{BvVQ>? MYfTRW5ITVJ?
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DB M2i3TWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{L0O2lEPTB;NUm4MlU4KG6P M1XX[XNCVkeHUh?=
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MV-4-11 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUH3OnhSUUN3ME22OVkvOSCwTR?= MVrTRW5ITVJ?
RPMI-8226 M4T3Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH7QcmRKSzVyPU[3PE4yQCCwTR?= NVvWPY1nW0GQR1XS
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DOHH-2 MnrIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUnSOGhPUUN3ME23NFYvPjhibl2= MoTOV2FPT0WU
D-423MG NH;WS4VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXP4XI85UUN3ME23NFYvQDlibl2= Ml\sV2FPT0WU
D-283MED NXrJN3VXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPmVmVoUUN3ME23NVQvOTRibl2= MVjTRW5ITVJ?
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NCI-H209 NH7hWGVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUTqfFB3UUN3ME23NlkvQSCwTR?= MoDmV2FPT0WU
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HAL-01 M{HaRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml;FTWM2OD17NkiuNFQhdk1? M3n4Z3NCVkeHUh?=
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LB1047-RCC NVrtN|E6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGDLPZlKSzVyPUGuNVg{OTJizszN M3fScHNCVkeHUh?=
VM-CUB-1 M1fFUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2O4PGlEPTB;MT6xPVExQCEQvF2= MYnTRW5ITVJ?
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CAL-54 MmDXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTRwMkKzPFEh|ryP MonWV2FPT0WU
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SF126 NX3MTVdmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnjNTWM2OD1zMD60O|I4KM7:TR?= M3\TWXNCVkeHUh?=
SK-MEL-1 Mo\aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M36wbGlEPTB;MUCuO|g{QCEQvF2= NYDmVWc{W0GQR1XS
ACN M2HuZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV\kdnVRUUN3ME2xNE45Pjd7IN88US=> MXfTRW5ITVJ?
BB30-HNC MkfMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4qzU2lEPTB;MUCuPFcyOSEQvF2= MUPTRW5ITVJ?
NCI-H747 MmXJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4\1NWlEPTB;MUCuPFc1PiEQvF2= NWfCc3BrW0GQR1XS
KYSE-150 MojPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX;JR|UxRTFzLkCzPFch|ryP NIO2OIJUSU6JRWK=
D-247MG NYjL[ohIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mlf4TWM2OD1zMT6xNFA6KM7:TR?= MmGxV2FPT0WU
BxPC-3 MkTYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFLTdXFKSzVyPUGxMlM4PDdizszN MnHnV2FPT0WU
SK-MEL-30 MmDsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn\wTWM2OD1zMT60NFc6KM7:TR?= NUfHNlJuW0GQR1XS
CAS-1 NVHuNJpkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHO0[XRKSzVyPUGxMlQyODlizszN NH\ESFdUSU6JRWK=
no-10 NUXRfnVrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX23SpF4UUN3ME2xNk4yOjF5IN88US=> MWDTRW5ITVJ?
NCI-H2405 M1rTZmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXjJOmlFUUN3ME2xNk4zODN4IN88US=> M{TiN3NCVkeHUh?=
KGN NVrnOWx4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFLsRVJKSzVyPUGyMlI6ODFizszN Mk\uV2FPT0WU
BB49-HNC NWjLWI8{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MofNTWM2OD1zMj6zPVU3KM7:TR?= NWjHdWQzW0GQR1XS
NCI-H1693 NFzkVmhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DaVGlEPTB;MUKuOVgyPiEQvF2= NFHGRVZUSU6JRWK=
RO82-W-1 MlO2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NELNb3hKSzVyPUGzMlA3PDlizszN MoXCV2FPT0WU
CAL-85-1 Mn7ES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFrST3JKSzVyPUGzMlQ{QTlizszN NIfQTW9USU6JRWK=
H-EMC-SS NFWxdlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2\o[WlEPTB;MUOuPVExPCEQvF2= Mk\xV2FPT0WU
SW1783 MmizS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlzxTWM2OD1zND6yN|kyKM7:TR?= NUXhcXZvW0GQR1XS
LB771-HNC MmLWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkHVTWM2OD1zND60NVkh|ryP MXLTRW5ITVJ?
HCC70 NGm1dXdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1XybGlEPTB;MUSuOFI{OyEQvF2= MXrTRW5ITVJ?
KNS-81-FD NFmwSo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrtOINjUUN3ME2xOE44ODR3IN88US=> Mn7oV2FPT0WU
KYSE-520 NVnMS4hlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHxTlRNUUN3ME2xOE46OjdizszN M1HEZ3NCVkeHUh?=
TGBC24TKB M2\0fmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3jzSWlEPTB;MUSuPVU{QSEQvF2= NUH2NYJGW0GQR1XS
SNB75 M37hbWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYntUGVkUUN3ME2xOU4xPzJ7IN88US=> M1O1dHNCVkeHUh?=
SW872 NHj1W2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGKzeINKSzVyPUG1MlIzOThizszN MVHTRW5ITVJ?
U031 NGDXVVZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4TORmlEPTB;MUWuPFAzQCEQvF2= NYnQ[IpmW0GQR1XS
ChaGo-K-1 M3S3bGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXXJR|UxRTF3LkizOVkh|ryP Ml\2V2FPT0WU
NCI-H441 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYHJR|UxRTF4LkCwN|kh|ryP NYm2[XdxW0GQR1XS
TE-12 NYLnZlJnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPieXpKUUN3ME2xOk4yQDd|IN88US=> MXHTRW5ITVJ?
NCI-H1755 NVzSZWxFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MonFTWM2OD1zNj61O|Mh|ryP NE\JU5pUSU6JRWK=
KNS-42 MUDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG\tOZFKSzVyPUG2MlY2QDlizszN NVnacZlVW0GQR1XS
SK-PN-DW NEi5Um1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRTF5LkSyNFEh|ryP M3HzO3NCVkeHUh?=
NCI-H226 MkfiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVLJR|UxRTF5LkmzOFUh|ryP MYnTRW5ITVJ?
K-562 M2PzUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYOxU4I2UUN3ME2xPE43OzF5IN88US=> MUTTRW5ITVJ?
A388 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWrJR|UxRTF7LkCzNFMh|ryP MmOxV2FPT0WU
ZR-75-30 NEn0W45Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVzsXGp5UUN3ME2xPU4xQTZ6IN88US=> MnXQV2FPT0WU
LU-135 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{W0N2lEPTB;MUmuNlY{PiEQvF2= NHjB[VlUSU6JRWK=
NCI-H2029 M1LTUWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NELlUWpKSzVyPUG5MlYxOjFizszN MUTTRW5ITVJ?
HT-1197 M3vOTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYDrW4l4UUN3ME2xPU46OjJ5IN88US=> M2fDVnNCVkeHUh?=
IGR-1 M1H0dGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTJyLkOxNVch|ryP MoLFV2FPT0WU
U-87-MG Moi4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIXlSmVKSzVyPUKwMlY1OTNizszN M3r4TnNCVkeHUh?=
NCI-H1304 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWnJR|UxRTJyLke1NFEh|ryP M3:2[XNCVkeHUh?=
RCC10RGB M2rMd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXPJR|UxRTJzLkK4Nlkh|ryP MoXuV2FPT0WU
NCI-H520 NEPLWYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUfJR|UxRTJ{Lke2NVEh|ryP MVrTRW5ITVJ?
SK-LU-1 M1W2eGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYnJR|UxRTJ|LkC3NVgh|ryP NYDsbJNrW0GQR1XS
UACC-893 MnTFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUPJR|UxRTJ|LkGwN|gh|ryP NVnjc2x5W0GQR1XS
NCI-H2347 M3nUbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTJ|LkK2O|Qh|ryP NXjEXpc1W0GQR1XS
U-118-MG NUi5UGNzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGPnOlJKSzVyPUKzMlczOTdizszN MWPTRW5ITVJ?
NCI-H596 NEfFNYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGjwWI5KSzVyPUKzMlkxPTVizszN NWfmfJFMW0GQR1XS
MZ1-PC M2q5eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXPYXZZkUUN3ME2yOE43PzV7IN88US=> MlfuV2FPT0WU
SW1417 NWr1OVlGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mmr5TWM2OD1{NT6yOFk5KM7:TR?= Moj5V2FPT0WU
NCI-H82 M3K2Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIHIS|RKSzVyPUK2MlQzQDhizszN NInHbXNUSU6JRWK=
COLO-678 Mo\6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NW\5Z|F1UUN3ME2yO{41QTF7IN88US=> MlTHV2FPT0WU
KURAMOCHI Mk[wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn[3TWM2OD1{OD6xNlYh|ryP NGnCbJpUSU6JRWK=
NCI-H1650 MkjzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnW1TWM2OD1|MD6xPVI1KM7:TR?= NXWzR29UW0GQR1XS
HCC1954 NGfZcGlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnTMTWM2OD1|MT6zOlY3KM7:TR?= MUTTRW5ITVJ?
GAK NFPIcldIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4ewb2lEPTB;M{KuNlYyPiEQvF2= MYjTRW5ITVJ?
YAPC NXW4cJVxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYXrNpM2UUN3ME2zN{44PjR6IN88US=> MnzyV2FPT0WU
NCI-H2228 NVnqVIp4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGD0PW5KSzVyPUO0MlM3ODVizszN NYTj[G1SW0GQR1XS
VMRC-RCZ NYLO[GJuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvkW5JKSzVyPUO2MlMxOjJizszN Mk[2V2FPT0WU
NMC-G1 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nybGlEPTB;M{euNFUzQSEQvF2= M1XoRXNCVkeHUh?=
D-336MG NIeyelBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWD2WXN6UUN3ME20NU45PDR{IN88US=> M1O4bXNCVkeHUh?=
UACC-62 NYPYWVNiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTR{LkW1NFUh|ryP NHHMSYZUSU6JRWK=
C-33-A M4HpdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{nZemlEPTB;NEWuN|I2PiEQvF2= MWPTRW5ITVJ?
HCC1937 NFXMTlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXiNoJKSzVyPUS3MlE6PThizszN NIPV[FBUSU6JRWK=
HDLM-2 MlvJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3K0ZWlEPTB;NEeuNlI5PyEQvF2= M3fNXXNCVkeHUh?=
NCI-H727 NGTZcnFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHhWFI4UUN3ME20O{4zPDVzIN88US=> MnLHV2FPT0WU
NY MnzJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWPJR|UxRTR7LkO5NFgh|ryP M4Ppc3NCVkeHUh?=
NCI-H2291 MlvQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWLJR|UxRTR7LkizNFEh|ryP M3TqPHNCVkeHUh?=

... Click to View More Cell Line Experimental Data

In vivo Administration of Vorinostat (~100 mg/kg/day) significantly inhibits the growth of CWR22 human prostate xenografts in nude mice with tumor reductions of 78%, 97% and 97%, at doses of 25 mg/kg/day, 50 mg/kg/day and 100 mg/kg/day, respectively, compared with control. Vorinostat induces the accumulation of acetylated core histones and prostate-specific antigen mRNA expression in CWR22 cells, resulting in higher levels of serum prostate-specific antigen than predicted from tumor volume alone. [2] Oral administration of Vorinostat (0.67g/L) crosses the blood-brain barrier, increases histone acetylation in the brain, and dramatically improves the motor impairment in the R6/2 mice model of Huntington's disease. [5]

Protocol

Kinase Assay:

[1]

+ Expand

Immunoprecipitation-HDAC assays:

The lysate of Jurkat cells is incubated for 1 hour on ice and cleared by centrifugation at 12,000 g for 10 minutes at 4 °C. Supernatants are precleared with 30 μL of 50% protein G-Sepharose slurry for 1 hour at 4 °C. Beads are pelleted by centrifugation and supernatants are incubated for 1 hour at 4 °C with 10 μg of IgG fraction from anti-HDAC1 or HDAC3 polyclonal antisera (preincubated 2 hours at room temperature with either the homologous or heterologous immunizing peptide). Both antisera are raised in rabbits against the carboxylterminal peptide of HDAC1 and HDAC3 by using synthetic peptides coupled to keyhole limpet hemocyanin. 30 μL of 50% protein G-Sepharose slurry is added for 1 hour at 4 °C. Immune complexes are pelleted by centrifugation and washed three times with 1 mL of lysis buffer. Beads are resuspended in 200 μL of HDAC buffer (20 mM Tris-HCl, pH 8.0/150 mM NaCl/10% glycerol), and the HDAC assay is performed with an 3H-acetylated peptide corresponding to amino acids 1-24 of histone H4. Released [3H]acetic acid is quantified by scintillation counting. For inhibitions studies, the immunoprecipitated complexes are preincubated with the different concentrations of Vorinostat for 30 minutes at 4 °C.
Cell Research:

[2]

+ Expand
  • Cell lines: LNCaP, PC-3, and TSU-Pr1
  • Concentrations: Dissolved in DMSO, final concentrations ~7.5 μM
  • Incubation Time: 1, 2, 3 and 4 days
  • Method:

    Cells are exposed to various concentrations of Vorinostat for 1, 2, 3 and 4 days. Cell viability is assessed by trypan blue dye exclusion.


    (Only for Reference)
Animal Research:

[2]

+ Expand
  • Animal Models: Male BALB/c nude (nu/nu) mice implanted with CWR22 tumor cells
  • Formulation: Dissolved in DMSO
  • Dosages: ~100 mg/kg/day
  • Administration: Injection i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 52 mg/mL (196.74 mM)
Ethanol 3 mg/mL (11.35 mM)
Water Insoluble
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 264.3
Formula

C14H20N2O3

CAS No. 149647-78-9
Storage powder
in solvent
Synonyms SAHA, MK0683, suberoylanilide hydroxamic acid

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03022565 Recruiting Uveal Melanoma University of Miami|University of Miami Sylvester Comprehensive Cancer Center July 2019 Early Phase 1
NCT03022565 Recruiting Uveal Melanoma University of Miami|University of Miami Sylvester Comprehensive Cancer Center July 2019 Early Phase 1
NCT03843528 Not yet recruiting Acute Myeloid Leukemia|Myelodysplastic Syndromes|Mixed Phenotype Acute Leukemia|Juvenile Myelomonocytic Leukemia Johns Hopkins All Children''s Hospital May 1 2019 Phase 1
NCT03843528 Not yet recruiting Acute Myeloid Leukemia|Myelodysplastic Syndromes|Mixed Phenotype Acute Leukemia|Juvenile Myelomonocytic Leukemia Johns Hopkins All Children''s Hospital May 1 2019 Phase 1
NCT03742245 Not yet recruiting Breast Cancer Metastatic|Breast Cancer Jenny C. Chang MD|AstraZeneca|The Methodist Hospital System April 1 2019 Phase 1
NCT03742245 Not yet recruiting Breast Cancer Metastatic|Breast Cancer Jenny C. Chang MD|AstraZeneca|The Methodist Hospital System April 1 2019 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Frequently Asked Questions

  • Question 1:

    What’s the recommendation about reconstitution of the compound for in vivo animal study?

  • Answer:

    We recommend the following vehicle for S1047: 2% DMSO, 40% PEG 300, 5% Propylene glycol, 1% Tween 80 at 5 mg/mL.

HDAC Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID