Vorinostat (SAHA)

Name: Vorinostat (SAHA)
Brand: Selleck Chemicals
SKU: S1047
Rating: 5 (401 reviews)

For research use only.

Catalog No.S1047 Synonyms: MK0683, Suberoylanilide hydroxamic acid

401 publications

CAS No. 149647-78-9

Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.

Selleck's Vorinostat (SAHA) has been cited by 401 publications

Cell, 2020, S0092-8674(20)31394-5

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Nat Biotechnol, 2020, 10.1038/s41587-019-0388-4

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Cell, 2019, 176(1-2):198-212

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Cell, 2019, 36(2):179-193

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Cancer Cell, 2019, 36(2):179-193

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Cell, 2018, 174(3):536-548.e21

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Cell Metab, 2018, 5;27(6):1294-1308.e7

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Nature, 2017, 551(7679):247-250

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Cancer Discov, 2017, 7(12):1450-1463

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Cancer Cell, 2016, 29(3):311-323

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Nat Biotechnol, 2015, 10.1038/nbt.3130

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Nat Med, 2015, 21(10):1163-71

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Nature, 2011, 10;471(7337):235-9.

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Nat Biotechnol, 2011, 29(3):255-65

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J Enzyme Inhib Med Chem, 2021, 36(1):34-47

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Sci China Life Sci, 2021, 10.1007/s11427-020-1855-4

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Transl Oncol, 2021, 14(1):100982

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J Dermatol Sci, 2021, S0923-1811(21)00005-0

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Adv Sci (Weinh), 2020, 7(21):2001364

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Nucleic Acids Res, 2020, 10.1093/nar/gkaa088

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EMBO Rep, 2020, 21:e50162

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J Neuroinflammation, 2020, 22;17(1):125

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ACS Infect Dis, 2020, 29

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Mol Cancer Res, 2020, 10.1158/1541-7786.MCR-19-0669

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Front Oncol, 2020, 10:296

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J Virol, 2020, 4;94(10) pii: e01923-19

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Biochem Pharmacol, 2020, 3;178:114067

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Cancer Cell Int, 2020, 19;20:58

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HIV Med, 2020, 21(11):747-757

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Am J Transl Res, 2020, 12(10):6187-6203

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BMC Microbiol, 2020, 20(1):28

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PLoS One, 2020, 15;15(5):e0232985

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Hum Cell, 2020, 10.1007/s13577-020-00420-z

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J Med Invest, 2020, 67(3.4):343-350

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Oncol Lett, 2020, 19(1):460-468

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Acta Pharm Sin B, 2020, 10(4):615-627

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Cancer Sci, 2020, 111(7):2374-2384

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Oncogene, 2020, 10.1038/s41388-020-01394-w

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Viruses, 2020, 12(10)E1067

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Oncogene, 2020, 10.1038/s41388-020-01408-7

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Br J Cancer, 2020, 10.1038/s41416-020-01191-y

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Mol Cancer Ther, 2020, 19(2):637-649

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Genes Dev, 2020, 34(5-6):428-445

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Biomater Sci, 2020, 10.1039/d0bm00119h

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Cell Cycle, 2020, 1-16

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Life Sci, 2020, 248:117469

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Chin Med J (Engl), 2020, 5;133(11):1304-1311

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Pharmaceuticals (Basel), 2020, 13(11)E345

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Pharm Biol, 2020, 58(1):231-238

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PLoS Biol, 2020, 18(12):e3000941

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Nat Commun, 2019, 10(1):4192

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J Pineal Res, 2019, 66(3):e12556

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Theranostics, 2019, 9(2):540-553

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Cancer Res, 2019, 79(7):1658-1670

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Cell Rep, 2019, 27(11):3331-3344

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Elife, 2019, 8

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Int J Cancer, 2019, 145(9):2496-2508

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Mol Ther, 2019, 27(5):1039-1050

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Cancers (Basel), 2019, 11(5)E645

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Cancers (Basel), 2019, 11(4)

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Clin Epigenetics, 2019, 11(1):85

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Acta Pharm Sin B, 2019, 9(5):937-951

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Cells, 2019, 8(12)

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Cancer Discov, 2019, 9(4):526-545

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Front Immunol, 2019, 9:3162

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Mol Cancer Ther, 2019, 18(5):900-908

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Cell Commun Signal, 2019, 17(1):4

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Oncologist, 2019, 24(8):e740-e748

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J Neurochem, 2019, 10.1111/jnc.14935

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Medicina (Kaunas), 2019, 55(2)

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Cancer Res, 2018, 78(10):2705-2720

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J Control Release, 2018, 275:117-128

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Cancer Lett, 2018, 432:121-131

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Acta Biomater, 2018, 71:261-270

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Sci Signal, 2018, 11(557)

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J Exp Clin Cancer Res, 2018, 37(1):153

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PLoS Pathog, 2018, 14(6):e1007061

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Oncoimmunology, 2018,

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Mol Cancer Ther, 2018, 17(12):2767-2779

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Cancer Sci, 2018, 109(8):2520-2531

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J Virol, 2018, 92(10)

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J Virol, 2018, 92(6)

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Front Microbiol, 2018, 9:2022

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J Cell Biochem, 2018, 119(5):4193-4204

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Retrovirology, 2018, 15(1):36

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Exp Cell Res, 2018, 371(1):231-237

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BMC Cancer, 2018, 18(1):466

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Oncol Rep, 2018, 39(1):264-270

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Ann Hematol, 2018, 97(5):839-849

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Biochem Biophys Res Commun, 2018, 498(3):481-486

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Proc Natl Acad Sci USA, 2017,

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Oncogene, 2017, 36(34):4859-4874

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Mol Ther Oncolytics, 2017,

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Cancer Discov, 2017, 7(12):1450-1463

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Eur J Med Chem, 2017, 127:531-553

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Pharm Res, 2017, 34(3):668-679

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Cancer Res Treat, 2017, 49(2):374-386

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Mol Immunol, 2017, 87:76-85

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Nucleus, 2017, 8(5):515-533

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Oncotarget, 2017,

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Cell Host Microbe, 2017, 21(5):569-579

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mBio, 2016, 7(6)

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Oncoimmunology, 2016,

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Mol Cancer Ther, 2016, 15(6):1364-75

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Mol Cell Proteomics, 2016, 15(6):2076-92

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Med Oncol, 2016, 33(2):11

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Inflammation, 2016, 39(1):39-46

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PLoS One, 2016, 11(8):e0160826

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Onco Targets Ther, 2016, 9:6843-6855

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Genet Mol Res, 2016, 15(4)

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Oncotarget, 2016,

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Nat Commun, 2015, 6:7888

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Cancer Res, 2015,

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Acta Neuropathol Commun, 2015, 3(1):22

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Epigenetics, 2015, 10.1080/15592294.2015.1039216

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Am J Cancer Res, 2015, 5(4):1337-52

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ACS Chem Biol, 2015, 10(10):2316-24

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Neuropharmacology, 2015, 99:67-78

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Biochemical Pharmacology, 2015, 94(2):79-90

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Biochem Pharmacol, 2015, 94(2):79-90

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Biochemical Pharmacology, 2015, 94(2):79-90

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Apoptosis, 2015, 20(7):975-85

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J Biol Chem, 2015, 290(8):5028-40

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BMC Cancer, 2015, 15(1):273

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Mol Vis, 2015, 21:502-14

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Mol Med Rep, 2015, 12(3):4291-4297

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Genes Cancer, 2015, 6(3-4):169-81

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Oncotarget, 2015, 6(18):15814-27

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Circ Heart Fail, 2015, 8(6):1094-104

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Sci Transl Med, 2014, 6(256):256ra135

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Acta Neuropathol, 2014, 128(2):291-303

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Nat Commun, 2014, 5:3361

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Clin Cancer Res, 2014, 10.1158/1078-0432.CCR-14-0384

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Int J Cancer, 2014, 135(7):1721-32

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Diabetes, 2014, 63(9):2924-34

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Oncogene, 2014, 33(25):3307-15

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Cancer Lett, 2014, 10.1016/j.canlet.2014.10.034

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Cancer Lett, 2014, 351(1):50-8

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PLoS Pathog, 2014, 10(4):e1004071

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Cell Death Dis, 2014, 5:e1134

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Cell Death Dis, 2014, 5:e1177

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Mol Cancer Ther, 2014, 13(12):2784-92

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J Mol Biol, 2014, 426(20):3442-53

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Hum Gene Ther, 2014, 25(7):599-608

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J Biol Chem, 2014, 289(14):9918-25

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Cancer Cell Int, 2014, 10.1186/s12935-014-0112-x

PubMed: ( click the link to review the publication )

Cancer Cell Int, 2014, 14(1):112

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Mol Cell Biol, 2014, 34(7):1280-9

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Proteomics Clin Appl, 2014, 8(11-12):924-31

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J Alzheimers Dis, 2014, 41(4):1193-205

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Virology, 2014, 462-463:328-39

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Cancer Biol Ther, 2014, 15(5):578-85

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Cancer Biol Ther, 2014, 15(3):305-16

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BMC Cancer, 2014, 14(1):373

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FEBS Lett, 2014, 588(9):1571-9

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PLoS One, 2014, 9(11):e113341

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Mol Med Rep, 2014, 10(5):2729-35

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Oncol Lett, 2014, 7(5):1673-1678

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Oncotarget, 2014, 5(14):5637-50

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Head Neck, 2014, 10.1002/hed.23822.

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Cytometry A, 2014, 85(1):78-87

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Pharmacology Research Perspectives, 2014, 10.1002/prp2.43

PubMed: ( click the link to review the publication )

Oncotarget, 2014, 5(15):5965-77

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Blood, 2013, 14;121(11):2038-50

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Blood, 2013, 123(5):706-16

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Nat Struct Mol Biol, 2013, 20(3):317-25

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Proc Natl Acad Sci USA, 2013, 111(3):E364-73

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PLoS Biol, 2013, 12(1):e1001758

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Cancer Res, 2013, 73(8):2428-34

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Cell Rep, 2013, 5(6):1679-89

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Int J Cancer, 2013, 132(9):2200-8

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Neuropsychopharmacology, 2013, 38(9):1674-84

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Oncogene, 2013, 33(33):4265-72

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Oncogene, 2013, 32(10):1316-29

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Cell Death Dis, 2013, 4:e733

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Cell Death Dis, 2013, 4:e951

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Br J Haematol, 2013, 160(2):188-98

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AIDS, 2013, 27(18):2853-62

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Apoptosis, 2013, 18(11):1348-1362

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J Biol Chem, 2013, 288(35):25593-602

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J Biol Chem, 2013, 288(20):14400-7

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PLoS Comput Biol, 2013, 9(2):e1002887

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Mol Carcinog, 2013, 52(3):218-28

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Front Pharmacol, 2013, 4:44

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Genes Brain Behav, 2013, 13(1):69-86

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Cell Cycle, 2013, 12(13):2073-83

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PLoS One, 2013, 8(6):e66130

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PLoS One, 2013, 8(1):e53766

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J Thromb Thrombolysis, 2013, 35(2):185-92

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Cell Death Dis, 2013, 4:e655

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Blood, 2012, 8;120(19):3958-67

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J Exp Med, 2012, 209(1):35-50

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Clin Cancer Res, 2012, 18(14):3822-33

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Clin Cancer Res, 2012, 18(2):408-16

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Cancer Res, 2012, 72(9):2251-61

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Cell Death Differ, 2012, 19(12):2029-39

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Eur J Cancer, 2012, 48(7):1096-107

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Diabetologia, 2012, 55(9):2421-31

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Mol Carcinog, 2012, 52(6):459-74

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Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description

Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.

Targets

HDAC ^[1]
(Cell-free assay)

~10 nM

In vitro

Vorinostat inhibits the activities of HDAC1 and HDAC3 with IC50 of 10 nM and 20 nM, respectively. Vorinostat also results in a marked hyperacetylation of histone H4. ^[1] Vorinostat inhibits the growth of three prostate cancer cell lines LNCaP, PC-3 and TSU-Pr1 at micromolar concentrations (2.5-7.5 μM), and induces dose-dependent cell death in LNCaP cells. ^[2] Vorinostat treatment in MCF-7 cells inhibits cell proliferation at an IC50 of 0.75 μM resulting in the accumulation of cells in the G1 and G2-M phase of the cell cycle. Vorinostat also induces differentiation in the estrogen receptor-negative cell line SKBr-3 and the retinoblastoma-negative cell line MDA-468. ^[3] Vorinostat treatment at 1 μM for 8 hours or more is sufficient to irreversibly induce apoptosis of human multiple myeloma (MM) cells. The gene expression profiles of Vorinostat treated MM cells are not hallmarked by global transcriptional activation, but by coordinated transcriptional changes of specific functional groups of genes such as cytokine-induced proliferative/survival signaling cascades, oncogenes-tumor suppressor genes, regulators of apoptosis, DNA synthesis-repair and cell cycle, and proteasome-ubiquitin function. ^[4]

Cell Data

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
H1299	MWrLbY5ie2ViQYPzZZk>		NFjuTnczOCCvaX6=	MkXFSG1UVw>?	M4PIdmlvcGmkaYTvdpkh[WO2aY\peJkh[WejaX7zeEBJTEGFIHXufplu\SC5aYToJGlEPTBib3[gNE4yQTRizszN	NX3Qc2J6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUS1NlE1OjJpPkG0OVIyPDJ{PD;hQi=>
HT1080	MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		M3zkRVczKGh?	M4nDUmROW09?	MULJR\|UxRTJwNDFOwG0>	NEHrN3A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkK3NFE4PSd-MUKyO\|AyPzV:L3G+
K562	MmfZT4lv[XOnIFHzd4F6		M2fC[lYhcA>?	MmLDSG1UVw>?	MmTpTY5pcWKrdHnvckBw\iCqdX3hckBpcXO2b37lJIRm[WOndInsZZNmKCivaYj0eZJmKG:oIFjERWMyKGGwZDDISGFEOilid3n0bEBKSzVyIH;mJFAvODFizszN	M3\yO\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{NEG5N\|gxLz5zMkSxPVM5ODxxYU6=
A20	NWrzdW5mU2mwYYPlJGF{e2G7		MnzINUBp	MWjEUXNQ	M{\HS4FvfGlvSFTBR\|Eh[WO2aY\peJkhf2m2aDDJR\|UxKG:oIECuNVEzKM7:TR?=	M1rRW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOU[wOFg6Lz5zMUm2NFQ5QTxxYU6=
Friend leukemic cell	MmLkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M{G2PVUxKM7:TR?=	NFXud\|I4OiCq	NUnQSoUzTE2VTx?=	MVfJR\|UxRTBwOUmg{txO	M13aSFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF{MUC5PVE{Lz5zMkGwPVkyOzxxYU6=
H1299	NEC3WotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NWnpUYozPzJiaB?=	NUH6dG1tTE2VTx?=	MWrJR\|UxRTdwMkSg{txO	M1TFT\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2NUKxOFIzLz5zNEWyNVQzOjxxYU6=
HeLa	MYPLbY5ie2ViQYPzZZk>		M3\4XFMxKG2rbh?=		NFPUb5FKdmirYnn0c5J6KGOxbnPlcpRz[XSrb36gZYdicW6\|dDDobZN1d26nIHTlZYNmfHmuYYPlJJdqfGhiSVO1NEBw\iByLkK4JO69VQ>?	M{m3WlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3NkCzPVQ6Lz5zNU[wN\|k1QTxxYU6=
LOX-IMVI	NGTEW2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		Mn\vOFghcA>?		NUTySYdXUUN3ME2xMlMh\|ryP	Mmq0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5MUW0O\|AoRjF3N{G1OFcxRC:jPh?=
NCI-H226	NXzl[Ww4T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NIL5cG41QCCq		M{LS[mlEPTB;Mj62JO69VQ>?	NF3STW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUexOVQ4OCd-MUW3NVU1PzB:L3G+
RXF-631L	NVrZcldCT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		MY[0PEBp		M4ji[GlEPTB;MjFOwG0>	NGfjRmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUexOVQ4OCd-MUW3NVU1PzB:L3G+
SNB-78	MnzlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MlHrOFghcA>?		MVTJR\|UxRTF4IN88US=>	MkX1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5MUW0O\|AoRjF3N{G1OFcxRC:jPh?=
St-4	MlTXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MUO0PEBp		NIXMVW9KSzVyPUWuNkDPxE1?	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTdzNUS3NEc,OTV5MUW0O\|A9N2F-
ARP1	NXjUTXNzTnWwY4Tpc44hSXO\|YYm=	NXfvfJQzOiEQvF2=	MoTzOkBp		MWfJcoR2[3Srb36gc4YhWDJzV1HGNUBo\W6nIHX4dJJme3Orb36=	NH24SIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEezOFgxPid-MUS3N\|Q5ODZ:L3G+
CEM	MoDIT4lv[XOnIFHzd4F6		NX;1cVV[OSCq	NXn5XotGTE2VTx?=	M{fHOGlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD6zN{DPxE1?	NVj2fYQzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[0NlAxPjRpPkG2OFIxODZ2PD;hQi=>
HCT15	MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NULQT5JVPDhiaB?=		NUS5bGJ2T0l3ME2wMlgzKM7:TR?=	MlmyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ5MkOyNlcoRjF4N{KzNlI4RC:jPh?=
NCI-H23	MnXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MYO0PEBp		Mo[0S2k2OD1yLkmyJO69VQ>?	M3G4VVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4N{KzNlI4Lz5zNkeyN\|IzPzxxYU6=
GM15850	MkTQT4lv[XOnIFHzd4F6	NHLI[XczNjVizszN	NXjS[GhtQTZiaB?=	Mmn0SG1UVw>?	NUTrUXllUW6qaXLpeIlwdiCxZjDISGFEKGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFMhdHm\|aX7lJFE1KGGlZYT5cIF1cW:wIH;mJGZZViCpZX7l	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl{MUO2O{c,OTZ7MkGzOlc9N2F-
GM15850	NVrQTFkzU2mwYYPlJGF{e2G7	M1W5ZlIvPSEQvF2=	M33Uflk3KGh?	MkmxSG1UVw>?	MYrJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKNDDsfZNqdmViNTDhZ4V1gWyjdHnvckBw\iCIWF6g[4Vv\Q>?	NWLzOplCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5NlE{PjdpPkG2PVIyOzZ5PD;hQi=>
GM15850	MlrjT4lv[XOnIFHzd4F6	MoX3Nk42KM7:TR?=	MmjyPVYhcA>?	NWTDOWJPTE2VTx?=	MX3Jcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKNDDsfZNqdmViMUKgZYNmfHmuYYTpc44hd2ZiRmjOJIdmdmV?	M1znfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUKxN\|Y4Lz5zNkmyNVM3PzxxYU6=
GM15850	NGS3ZVVMcW6jc3WgRZN{[Xl?	NGW4clMzNjVizszN	MXS5OkBp	M1jVbmROW09?	M3rpVGlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDobZN1d26nIFizJIx6e2mwZTC5JIFk\XS7bHH0bY9vKG:oIF\YUkBo\W6n	NWDpSI9ZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5NlE{PjdpPkG2PVIyOzZ5PD;hQi=>
GM15850	NUPoUm1lU2mwYYPlJGF{e2G7	Mn:5Nk42KM7:TR?=	NY\LWGU4QTZiaB?=	MmK1SG1UVw>?	NUnVPXc6UW6qaXLpeIlwdiCxZjDISGFEKGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFQhdHm\|aX7lJFgh[WOndInsZZRqd25ib3[gSnhPKGenbnW=	MYG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl{MUO2O{c,OTZ7MkGzOlc9N2F-
GM15850	MUDLbY5ie2ViQYPzZZk>	NEjD[FAzNjVizszN	Mo\GPVYhcA>?	NYLFVpo4TE2VTx?=	MYXJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKNDDsfZNqdmViMU[gZYNmfHmuYYTpc44hd2ZiRmjOJIdmdmV?	M2ThNlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUKxN\|Y4Lz5zNkmyNVM3PzxxYU6=
U937	MnTBSpVv[3Srb36gRZN{[Xl?	NWfjPFFpPSEQvF2=	NXPtUndSOjRiaB?=	NUPGc\|h3TE2VTx?=	NULzS\|ZFS2WubDDjfYNt\SCjcoLld5Qh[nliYXPjeY12dGG2aX;uJIF1KFNxR{KvUUBxcGG\|ZR?=	NF7SfWg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{CwOFcyQCd-MUewNFQ4OTh:L3G+
H661	Mn31S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MoL3OFghcA>?		NGLCVIZKSzVyPUGg{txO	MoDEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTdzNUewNFkoRjF5MUW3NFA6RC:jPh?=
U937	NYfOTYpHTnWwY4Tpc44hSXO\|YYm=	NX;yN4o1PSEQvF2=	NEjmXnUzPCCq	NGLVXFNFVVOR	M3XE[Glv[3KnYYPlJIlvKGirc4TvcoUhUDNiYXPleJlt[XSrb36=	NXXYc5Q2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0OFc4PTBpPkG3OFQ4PzVyPD;hQi=>
U937	M2i2SWZ2dmO2aX;uJGF{e2G7	MUCxJO69VQ>?	M1n4b\|I1KGh?	MkLQSG1UVw>?	NXH5XGNCTW[oZXP0JI9vKGGucHjhJJR2[nWuaX6gZYNmfHmuYYTpc44>	NF2zVYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S0O\|c2OCd-MUe0OFc4PTB:L3G+
RAW264.7	NFHidWhHfW6ldHnvckBCe3OjeR?=	NUDreZJPOTBizszN	NEm2fJIzPGh?	M4HZcpdifGW{	MlPZTY5pcWKrdHnvckBw\iCOUGOtd5RqdXWuYYTl[EBVVk[jbIDoZUBxem:mdXP0bY9vKHerdHigTWM2OCCxZjCwMlc3KM7:TR?=	NFzy[Gs9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{S3O\|UyQCd-MUe0O\|c2OTh:L3G+
RAW264.7	M3q3SWZ2dmO2aX;uJGF{e2G7	NH6xT5AyOCEQvF2=	M{LZV\|I1cA>?	MYT3ZZRmeg>?	M33XTWlvcGmkaYTpc44hd2ZiTGDTMZN1cW23bHH0[YQhVk9icILv[JVkfGmxbjD3bZRpKEmFNUCgc4YhOS56ODFOwG0>	MoPHQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd2N{e1NVgoRjF5NEe3OVE5RC:jPh?=
SC9	M13QTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		MWC0PEBp		NVjNZYV[UUN3ME2wMlYxPiEQvF2=	NY\sR2tmRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1NFczOTlpPkG3OVA4OjF7PD;hQi=>
ST14A	Ml;NSpVv[3Srb36gRZN{[Xl?	NIjOTI82KG6P	MYG3NkBp		M4\L[mlv[3KnYYPlJIlvKFKHU2SvUnJUTiCvUl7BJIV5eHKnc4Ppc44>	NIS4VZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W2OVk6Oyd-MUe1OlU6QTN:L3G+
ST14A	MlrtSpVv[3Srb36gRZN{[Xl?	MVy1JI5O	NF;pUpU4OiCq		MmqwTY5kemWjc3WgbY4hTTKIMTDtVm5CKGW6cILld5Nqd25?	NWjVXlNjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1OlU6QTNpPkG3OVY2QTl\|PD;hQi=>
PANC1	MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	M1\ve\|Eh\|ryP			MnHpTWM2OD1yLkS1JO69VQ>?	MkWyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3OEi3OFQoRjF5NUi4O\|Q1RC:jPh?=
PANC1	Mk\BSpVv[3Srb36gRZN{[Xl?	NXjyT25MOSEQvF2=	MUSyOEBp		MV;JcoNz\WG\|ZTDpckBxOjGZQV[xM2NKWDFiZ3Xu[UBmgHC{ZYPzbY9v	M{DaVlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUi4O\|Q1Lz5zN{W4PFc1PDxxYU6=
PANC1	NHnUT3VHfW6ldHnvckBCe3OjeR?=	MU[xJO69VQ>?	MUSyOEBp		MXfJcoNz\WG\|ZTDpckBxOjetaYCxJIdmdmViZYjwdoV{e2mxbh?=	NGjOXVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W4PFc1PCd-MUe1PFg4PDR:L3G+
PT45	MmHmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NI\S[\|dKSzVyPUSg{txO	NFHUWm49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{W4PFc1PCd-MUe1PFg4PDR:L3G+
ACHN	NXHyc5JMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		M1HXZ\|IyKGh?		M1TTOmdKPTB;MD62OUDPxE1?	NXizcoVsRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2OlI3ODZpPkG3OlYzPjB4PD;hQi=>
PC3	NFvKflVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NVPtS\|hFOjFiaB?=		M1nvUmdKPTB;MD63NUDPxE1?	NFzQblE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[2NlYxPid-MUe2OlI3ODZ:L3G+
A431	MnTjR5l1d3SxeHnjJGF{e2G7		MVK3NkBp		MV;JR\|UxRTRwMjFOwG0>	NYG0RXZvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2PVE4PjNpPkG3OlkyPzZ\|PD;hQi=>
Cal27	MWPDfZRwfG:6aXOgRZN{[Xl?		M3HXVFczKGh?		NHPJNXBKSzVyPUOuNkDPxE1?	MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ7MUe2N{c,OTd4OUG3OlM9N2F-
CCRF-CEM	M3\PTmN6fG:2b4jpZ{BCe3OjeR?=		NWDKPIZ6PzJiaB?=		MXHJR\|UxRTBwODFOwG0>	MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ7MUe2N{c,OTd4OUG3OlM9N2F-
EOL1	NGnxfZJEgXSxdH;4bYMhSXO\|YYm=		NXvMTI14PzJiaB?=		Mo[0TWM2OD1zIN88US=>	NY\ZUXE4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2PVE4PjNpPkG3OlkyPzZ\|PD;hQi=>
Hec-1-A	MoL6R5l1d3SxeHnjJGF{e2G7		NIXvfWQ4OiCq		NWnBNWxyUUN3ME2xJO69VQ>?	NXXCUFdvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2PVE4PjNpPkG3OlkyPzZ\|PD;hQi=>
HeLa	M2jvfGtqdmG\|ZTDBd5NigQ>?	NILYcXMyOCEQvF2=	NYHHW2ZwOzBibXnu	MmTMSG1UVw>?	MnnDTY5pcWKrdHnvckBw\iC{ZXPvcYJqdmGwdDDISGFEOSC5aYToJGlEPTBib3[gNE4xOiEQvF2=	NIjLToQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[5NVc3Oyd-MUe2PVE4PjN:L3G+
RKOp21	MlzKR5l1d3SxeHnjJGF{e2G7		NFzHUlI4OiCq		MVnJR\|UxRTFwN{[g{txO	NUL5dHgyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe2PVE4PjNpPkG3OlkyPzZ\|PD;hQi=>
Saos2	NYfubVh1S3m2b4TvfIlkKEG\|c3H5		MWO3NkBp		M{PvcWlEPTB;Mj6zJO69VQ>?	MnHhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4OUG3OlMoRjF5NkmxO\|Y{RC:jPh?=
SK-OV-3	M4\Wc2N6fG:2b4jpZ{BCe3OjeR?=		NIHIR5M4OiCq		Mmn0TWM2OD1{LkKg{txO	NFnP[os9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{[5NVc3Oyd-MUe2PVE4PjN:L3G+
U87MG	MXHDfZRwfG:6aXOgRZN{[Xl?		M3jXdVczKGh?		M1PU[2lEPTB;MUGg{txO	MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzZ7MUe2N{c,OTd4OUG3OlM9N2F-
NUGC3	M4fU[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MmfZOUDPxE1?	MViyOEBp		NITpR2pIUTVyPUKuO\|kh\|ryP	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzlyNEi0N{c,OTd7MES4OFM9N2F-
CCD1059SK	M3WzSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NV32[3YxUUN3ME20N{42KM7:TR?=	NWfpUWJTRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFc2PTRpPkG4NlQ4PTV2PD;hQi=>
HepG2	MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MWfJR\|UxRTBwMECwOkDPxE1?	NVPldodMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFc2PTRpPkG4NlQ4PTV2PD;hQi=>
HT1080	NITQdFNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MYLJR\|UxRTBwMECyOEDPxE1?	MoHmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh{NEe1OVQoRjF6MkS3OVU1RC:jPh?=
SKBR3	NUPzd2hrT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NVX2ZWF{UUN3ME2wMlgh\|ryP	NYTudW5lRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUiyOFc2PTRpPkG4NlQ4PTV2PD;hQi=>
SNB78	MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M{XFfWVEPTB;MU[g{txO	NFX4bnI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK0O\|U2PCd-MUiyOFc2PTR:L3G+
SQ20B	M{exd2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NXPINJhzUUN3ME2zJO69VQ>?	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
St4	M3jhO2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MVnFR\|UxRTVwMjFOwG0>	MUm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDJ2N{W1OEc,OTh{NEe1OVQ9N2F-
MCF7	MYXGeY5kfGmxbjDBd5NigQ>?	MVSxNEDPxE1?	MlXYPEBp	MXHEUXNQ	MYnJcoR2[3Srb36gc4YhfHWkdXzpckBpgXCncnHj[ZR6dGG2aX;u	NEL2SZU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOEK2PVIzPid-MUiyOlkzOjZ:L3G+
HEK293	MmjsT4lv[XOnIFHzd4F6		NGfPbIUyKGh?	MoHNSG1UVw>?	MVzJcohq[mm2aX;uJI9nKEiGQVOxJJdqfGhiSVO1NEBw\iByLkCzJO69VQ>?	MlTjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|MEi1OlMoRjF6M{C4OVY{RC:jPh?=
HEK293	NVz6fZlTU2mwYYPlJGF{e2G7		MX6xJIg>	M1TnTmROW09?	MkLhTY5pcWKrdHnvckBw\iCKRFHDN{B4cXSqIFnDOVAhd2ZiMD6wOVch\|ryP	NYW5UWh5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUizNFg2PjNpPkG4N\|A5PTZ\|PD;hQi=>
G401	NEHSZ3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		MYG3NkBp	NITsXZNFVVOR	MXnJR\|UxRTFizszN	M{HLcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{ewN\|c{Lz5zOEO3NFM4OzxxYU6=
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Human renal epithelial cell	Mk\PS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MWi3NkBp	NUDOc3NoTE2VTx?=	MUjJR\|UxRTF2IN88US=>	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN5MEO3N{c,OTh\|N{CzO\|M9N2F-
U937	M2C1WmZ2dmO2aX;uJGF{e2G7	NF;6d2Y2KM7:TR?=	MWqyOEBp		MVPJcoR2[3Srb36gc4YhcGm\|dH;u[UBJPCCqeYDldoFk\XS7bHH0bY9v	MUS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN6MUKzPEc,OTh\|OEGyN\|g9N2F-
U937	NYDGWYpXTnWwY4Tpc44hSXO\|YYm=	NWfuS\|U2PSEQvF2=	MnnYNlQhcA>?		NYTLO45JUW6mdXP0bY9vKG:oIHHsdIhiKHS3YoXsbY4hcHmyZYLhZ4V1gWyjdHnvci=>	M1TRUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{ixNlM5Lz5zOEO4NVI{QDxxYU6=
U937	M3TSOmZ2dmO2aX;uJGF{e2G7	NH:zdFI2KM7:TR?=	NHe5cXMzPCCq		MlrqTY5lfWO2aX;uJI9nKHB{MTDlfJBz\XO\|aX;u	M1Tp[VxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6M{ixNlM5Lz5zOEO4NVI{QDxxYU6=
U937	NGnF[IxHfW6ldHnvckBCe3OjeR?=	M{DFRlUh\|ryP	MVGyOEBp		MUHJcoR2[3Srb36gc4Yh[XCxcITvd4l{	MnTIQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|OEGyN\|goRjF6M{ixNlM5RC:jPh?=
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U937	MVXLbY5ie2ViQYPzZZk>	NEPCOJM2KM7:TR?=	MmHuNlQhcA>?		MWrJcohq[mm2aX;uJI9nKGi3bXHuJGhFSUNz	Mn7IQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh\|OEGyN\|goRjF6M{ixNlM5RC:jPh?=
U937	NVfj[YdOU2mwYYPlJGF{e2G7	MmjnOUDPxE1?	NIruXIUzPCCq		MlTZTY5pcWKrdHnvckBw\iCqdX3hckBJTEGFNB?=	MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDN6MUKzPEc,OTh\|OEGyN\|g9N2F-
HEK-TE	NIHs[pJHfW6ldHnvckBCe3OjeR?=	M3zuVlUh\|ryP	M2TF[VZp	NF:3dlhFVVOR	NW\Fb\|lEUW6lcnXhd4UhcW5iaHnzeI9v\SCKMzDhZ4V1gWyjdHnvci=>	NWC5cWVnRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUi0N\|QyPDRpPkG4OFM1OTR2PD;hQi=>
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Human microvascular epithelial cell	M2LQWGN6fG:2b4jpZ{BCe3OjeR?=	M4fOVVUxKM7:TR?=	MnXxO\|IhcA>?	M1vubmROW09?	NEXpSI9KSzVyPUGuN{DPxE1?	NEDkXow9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOES5OFQ3Oyd-MUi0PVQ1PjN:L3G+
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Mia Paca2	NVfzXmlqS3m2b4TvfIlkKEG\|c3H5	MnLnOVAh\|ryP	NUO1R3E2PzKq	MnW4SG1UVw>?	NGXMcmRKSzVyPUGuNUDPxE1?	M{f4b\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NEm0OFY{Lz5zOES5OFQ3OzxxYU6=
Panc 04.03	NYHTNVdMS3m2b4TvfIlkKEG\|c3H5	NYTpUYp7PTBizszN	MYq3Nog>	NIDWeoZFVVOR	MkG4TWM2OD1zLkKg{txO	MYK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQDR7NES2N{c,OTh2OUS0OlM9N2F-
SU 86.86	NI\1UG1EgXSxdH;4bYMhSXO\|YYm=	MnPMOVAh\|ryP	NUnTfXNYPzKq	MnzJSG1UVw>?	M4DDUWlEPTB;MT6zJO69VQ>?	MoD0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTh2OUS0OlMoRjF6NEm0OFY{RC:jPh?=
HEK293	M3W1SmtqdmG\|ZTDBd5NigQ>?		M1nPOFMhcA>?		NWW1NZNrUW6qaXLpeIlwdiCxZjDy[YNwdWKrbnHueEBJTEGFNjDlfJBz\XO\|aX;uJJdqfGhiSVO1NEBw\iByLkCxPUDPxE1?	M{DweFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF6NUW4OlY6Lz5zOEW1PFY3QTxxYU6=
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RAW264.7	NHfhTlVHfW6ldHnvckBCe3OjeR?=	M{WwZVIh\|ryP	Mmq0N{42KGh?	MUnEUXNQ	M4fqRmFvfGmvYXzhdolidCCjY4Tpeol1gSCjZ3HpcpN1KFCuYYPtc4RqfW1iZnHsZ4lx[XK3bTDpcoZm[3SnZDD3bZRpKEmFNUCgc4YhOC5zMTFOwG0>	NEDqdGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOE[0OFk3QSd-MUi2OFQ6Pjl:L3G+
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HEK293	MWjGeY5kfGmxbjDBd5NigQ>?	Mm\NNVAh\|ryP	MmXrNlQhcA>?	NVXvNZFlTE2VTx?=	NH\KOpNC[3SrdnH0bY9vKG:oIH3veZNmKFewdEPhJJNq\26jbHnu[{BmgHC{ZYPz[YQ>	NX\BUVdFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwNlI3PzVpPkG5NFIzPjd3PD;hQi=>
HCT116	NGLrSGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NFPPU\|Q4Omh?	NEW1eHNFVVOR	MnThTWM2OD1yLkOxJO69VQ>?	Mn;1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyOESyPVQoRjF7MEi0Nlk1RC:jPh?=
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IGROV1	NH:1cplIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		MkjEO\|IhcA>?	NVL3eVU{TE2VTx?=	M4TDVmlEPTB;Mj6yJO69VQ>?	NH\ESmk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC4OFI6PCd-MUmwPFQzQTR:L3G+
IGROV1	MnHNT4lv[XOnIFHzd4F6	MXK1JO69VQ>?	NXiyO4pbOjRiaB?=	NY\2PYI3TE2VTx?=	M{\MUmlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCyNUOgZYNmfHmuYYTpc44>	M1X0RVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7MEi0Nlk1Lz5zOUC4OFI6PDxxYU6=
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IGROV1	MlTuT4lv[XOnIFHzd4F6	MV6xNEDPxE1?	NHP6UWE1KGh?	NV\G[294TE2VTx?=	NGHCUG1KdmirYnn0bY9vKG:oIFjERWM3KGG\|c3Xzd4VlKGG\|IHHsdIhiNXS3YoXsbY4h[WOndInsZZRqd25?	MljkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTlyOESyPVQoRjF7MEi0Nlk1RC:jPh?=
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T24	M1\pWmtqdmG\|ZTDBd5NigQ>?		NYXaNFlCOyCq		NIjU[nFKdmS3Y4Tpc44hd2ZiYXzwbIEhfHWkdXzpckBi[2W2eXzheIlwdiC5aYToJGVEPTBib3[gNE4zPSEQvF2=	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB6NEO5OUc,OTlyOESzPVU9N2F-
HeLa	M{jWTGtqdmG\|ZTDBd5NigQ>?		MlztNVUhdWmw	MnvSSG1UVw>?	NFnJdmZKdmirYnn0bY9vKG:oIFjERWM3KG[{b32gcpVkdGWjcjDlfJRz[WO2IIfpeIghUUN3MDDv[kAxNjB6NUWg{txO	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTB7M{i4OEc,OTlyOUO4PFQ9N2F-
HeLa	M2TZbWtqdmG\|ZTDBd5NigQ>?		NVHZN\|FVOTVibXnu	MnuxSG1UVw>?	NIDVdIZKdmirYnn0bY9vKG:oIFjERWM5KG[{b32gcpVkdGWjcjDlfJRz[WO2IIfpeIghUUN3MDDv[kAyNjh4IN88US=>	NVXvOWwyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUmwPVM5QDRpPkG5NFk{QDh2PD;hQi=>
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SKMES1	NHPvfZBEgXSxdH;4bYMhSXO\|YYm=		M4DobVczKGh?	MYDEUXNQ	M2rjcWlEPTB;Mj60NkDPxE1?	NGS1eIU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUC5N\|g5PCd-MUmwPVM5QDR:L3G+
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SHSY5Y	NW\0T21NU2mwYYPlJGF{e2G7	MVixNEDPxE1?	Mm\IOFghcA>?	NF7adJpFVVOR	NIKyb2ZKdmirYnn0bY9vKG:oIFjERWMhf2m2aDDJR\|UxKG:oIECuOUDPxE1?	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTN2NEG3OUc,OTl\|NESxO\|U9N2F-
SHSY5Y	NYHncYlWS3m2b4TvfIlkKEG\|c3H5	NX3oPVdYOTBizszN	MVK0PEBp	M1K5NmROW09?	NWGyblZ3UUN3ME2yJO69VQ>?	NEHHS4c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUO0OFE4PSd-MUmzOFQyPzV:L3G+
DMS114	M3vVV2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7		MXy0PEBp		MUXHTVUxRTJwOTFOwG0>	NWTq[2dRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm0NVkzODVpPkG5OFE6OjB3PD;hQi=>
HBC5	NEG1WZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NVfZ[FRmPDhiaB?=		MoH3S2k2OD1zNzFOwG0>	MV[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTRzOUKwOUc,OTl2MUmyNFU9N2F-
SNB78	M1u5d2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7		M3zVUlQ5KGh?		NVXIPYNzT0l3ME2xOkDPxE1?	M2PvWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7NEG5NlA2Lz5zOUSxPVIxPTxxYU6=
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CFBE41o-	NGXyeHpMcW6jc3WgRZN{[Xl?	NEnyXYQ2KM7:TR?=	MVy0JIg>	MYfEUXNQ	M{GydGlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDteZRidnRiRnTlcJRiPTB6IFPGWHIheHKxdHXpckBt\X[nbB?=	NV7keZZXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OlY4QDlpPkG5PVY3Pzh7PD;hQi=>
CFBE41o-	NXTPVJNLU2mwYYPlJGF{e2G7	NH61bVMyKM7:TR?=	NUjr[GpCOSCq	Ml73SG1UVw>?	NWXMRY05UW6qaXLpeIlwdiCxZjDISGFEKGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJGNHXFJicILveIVqdiCuZY\lcC=>	MlK4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Nk[3PFkoRjF7OU[2O\|g6RC:jPh?=
Primary bronchial epithelial cell	NVvCb4hnU2mwYYPlJGF{e2G7	MkK3NUDPxE1?	MWKyOEBp	MWHEUXNQ	M4PP[mlvcGmkaYTpc44hd2ZiSFTBR\|ch[XO\|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJI12fGGwdDDG[IVtfGF3MEigR2ZVWiCycn;0[YlvKGGyaXPhcEB{fXKoYXPlJIxw[2GuaYrheIlwdg>?	MYi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl4Nke4PUc,OTl7Nk[3PFk9N2F-
CFBE41o-	NFHRPJFMcW6jc3WgRZN{[Xl?	MYOxJO69VQ>?	MmOyOUBl[Xm\|	MYfEUXNQ	MWTJcohq[mm2aX;uJI9nKEiGQVO3JIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIIP0ZYJqdGm8YYTpc44hd2ZiRnTlcJRiPTB6IFPGWHIheHKxdHXpci=>	M1vxdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OU[2O\|g6Lz5zOUm2Olc5QTxxYU6=
CFBE41o-	NFSyZXdMcW6jc3WgRZN{[Xl?	NXfGNY9[OSEQvF2=	M2PHdlI1KGh?	MoXFSG1UVw>?	NXXHXIRlUW6qaXLpeIlwdiCxZjDISGFEPyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBH\GWudHG1NFghS0[WUjDwdo91\WmwIITyZYZncWOtaX7n	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl4Nke4PUc,OTl7Nk[3PFk9N2F-
CFBE41o-	MX7LbY5ie2ViQYPzZZk>	MnrwOUDPxE1?	Mo\kNlQhcA>?	MoL0SG1UVw>?	NHTqc\|ZKdmirYnn0bY9vKG:oIFjERWM4KGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJJJifGVib3[gcYF1fXKjdHnvckBw\iCFRmTSJJBzd3SnaX6geI8hcXS\|IHfsfYNw\m:{bR?=	MoL0QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Nk[3PFkoRjF7OU[2O\|g6RC:jPh?=
CFBE41o-	NWXqPWNbU2mwYYPlJGF{e2G7	NEm3PVE2KM7:TR?=	MVWyOEBp	NGDUV41FVVOR	M4rqPWlvcGmkaYTpc44hd2ZiSFTBR\|ch[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gd5Ri[mmuaYT5JI9nKE[mZXz0ZVUxQCCFRmTSJJBzd3SnaX6=	MYq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yQTl4Nke4PUc,OTl7Nk[3PFk9N2F-
CFBE41o-	NHSxN3JMcW6jc3WgRZN{[Xl?	MWq1JO69VQ>?	NXvLflJ3OjRiaB?=	MY\EUXNQ	NEjDSZhKdmirYnn0bY9vKG:oIFjERWM4KGG\|c3Xzd4VlKGG\|IHPvdpJm[3Srb36gc4YhdXW2YX70JGZl\Wy2YUWwPEBETlSUIITyZYZncWOtaX7nJJRwKGOnbHygd5Vz\mGlZTDhd{BodHmlb3\vdo0>	M2nCUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF7OU[2O\|g6Lz5zOUm2Olc5QTxxYU6=
Primary bronchial epithelial cell	M3;IfWtqdmG\|ZTDBd5NigQ>?	MXq1JO69VQ>?	MVuyOEBp	Mk\mSG1UVw>?	MnXsTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W\|c3XkJIF{KGmwZIXjeIlwdiCxZjDteZRidnRiRnTlcJRiPTB6IFPGWHIheHKxdHXpckBieGmlYXygd5Vz\mGlZTDsc4NidGm8YYTpc44>	MoixQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Nk[3PFkoRjF7OU[2O\|g6RC:jPh?=
Primary bronchial epithelial cell	NVT5VGQ3U2mwYYPlJGF{e2G7	NIXNfpgyKM7:TR?=	NYXHNm4xQTZiaB?=	M4O4d2ROW09?	NFXIZXpKdmirYnn0bY9vKG:oIFjERWM4KGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIHnuJJNpd3K2IHPpdoN2cXRiY4XydoVvfHN?	NVTnbnY3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OlY4QDlpPkG5PVY3Pzh7PD;hQi=>
BxPC3	NXvjd3d7T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		MXq3NkBp		NFnjTGJKSzVyPUKuO{DPxE1?	MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{e3PEc,OjBzNEO3O\|g9N2F-
CAL27	NYTn[mJLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NXjte\|N{PzJiaB?=		MnvGTWM2OD1\|LkeyJO69VQ>?	NIfnUlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N\|c4QCd-MkCxOFM4Pzh:L3G+
Capan1	M1LFfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		M4PDSFczKGh?		MV;JR\|UxRTdwMzFOwG0>	NX7W[mh[RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM4PzhpPkKwNVQ{Pzd6PD;hQi=>
HCC827	MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MYO3NkBp		M1fMNWlEPTB;MT64JO69VQ>?	MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{e3PEc,OjBzNEO3O\|g9N2F-
LNCAP	NX71PW16T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		MmTaO\|IhcA>?		MlTLTWM2OD1yLkW4JO69VQ>?	NXT6S2VvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM4PzhpPkKwNVQ{Pzd6PD;hQi=>
NCI-H2122	M{\zOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NVHCW5lHPzJiaB?=		M4jYOGlEPTB;Nz61JO69VQ>?	NIGzXFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N\|c4QCd-MkCxOFM4Pzh:L3G+
NCI-H358	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		M2TuVFczKGh?		NYXWb25qUUN3ME2yMlUh\|ryP	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{e3PEc,OjBzNEO3O\|g9N2F-
SCC15	NXfkfopET3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NIfCeXA4OiCq		M1O4c2lEPTB;Mj60PUDPxE1?	Mnv5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO3O\|goRjJyMUSzO\|c5RC:jPh?=
SCC25	MoTWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		NHTLcow4OiCq		MYjJR\|UxRTFwOUOg{txO	MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{e3PEc,OjBzNEO3O\|g9N2F-
SKHEP1	M4[5fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		M3GzNlczKGh?		NGfKbXpKSzVyPUOuOFYh\|ryP	M2TiPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzO\|c5Lz5{MEG0N\|c4QDxxYU6=
BJ	NYqwV4RmT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M{P4b\|ExOCEQvF2=	NFG0ZZQ1QCCq	NUPE[lY4TE2VTx?=	MUnHdo94fGhiaX7obYJqfGmxbjDheEAxNjB3IITvJFExOCC3TR?=	MUW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
MUF	NHznc4xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NYjkS4hvOTByIN88US=>	MmD3OFghcA>?	MYHEUXNQ	NWPI[ZJIT3Kxd4ToJIlvcGmkaYTpc44h[XRiMD6wOUB1dyBzMECgeW0>	NYjHdmx6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
Caco-2	NHH2RmZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MkXUNVAxKM7:TR?=	NUTz[4dmPDiq	M4T6R2ROW09?	Mn\0TWM2OD16LkGg{txO	NWDObYk4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
Caco-2	MWHLbY5ie2ViQYPzZZk>	NETLWWs2KM7:TR?=	NVXMT3M5PCCq	MXrEUXNQ	MV\JcoR2[3Srb36gc4YhUESDQz3t[YRq[XSnZDDobZN1d26nIHHj[ZR6dGG2aX;uJGlEPTBib3[gNE4xOzdizszN	NW[2dZlCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
Caco-2	NWfCcmVxU2mwYYPlJGF{e2G7	M4HIT\|AvOzF4IH7NJJRwKDNzLk[g{txO	Mmr5OEBp	NX;OWZp2TE2VTx?=	NUL5PZBQTXhidnn2c{BqdmirYnn0bY9vKG:oIHj1cYFvKEiGQVOxJJdqfGhiSVO1NEBw\iByLkGxPUDPxE1?	M1TFTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzPFQxLz5{MEG0N\|g1ODxxYU6=
Caco-2	NITEXJpMcW6jc3WgRZN{[Xl?	M33PXlAvOzF4IH7NJJRwKDNzLk[g{txO	NVP1WoVRPCCq	NHvPO3dFVVOR	NV3LU2FtTXhidnn2c{BqdmirYnn0bY9vKG:oIHj1cYFvKEiGQVOzJJdqfGhiSVO1NEBw\iByLkG0O{DPxE1?	NXPtOI1XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
Caco-2	NXnNOI9zU2mwYYPlJGF{e2G7	MVK2MlMzKG6PIITvJFY{OiEQvF2=	Mn7xOEBp	MlziSG1UVw>?	NF;LcY1GgCC4aY\vJIlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{Wge4l1cCCLQ{WwJI9nKDF{Lk[g{txO	MmS1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
Caco-2	NUXVUJpQU2mwYYPlJGF{e2G7	MmjmOk4{OiCwTTD0c{A3OzJizszN	MnzqOEBp	Mk\zSG1UVw>?	MYHFfEB3cX[xIHnubIljcXSrb36gc4YhcHWvYX6gTGRCSzdid3n0bEBKSzVyIH;mJFQyKM7:TR?=	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
Caco-2	MYnLbY5ie2ViQYPzZZk>	MY[wMlMyPiCwTTD0c{A{OS54IN88US=>	NV\0UpJYPCCq	M{HK[WROW09?	Mo\jSZghfmm4bzDpcohq[mm2aX;uJI9nKGi3bXHuJGhFSUN4	MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
HepG2	NGTJTnpMcW6jc3WgRZN{[Xl?	MWK1JO69VQ>?	MmP0Oog>	NFPzUlhFVVOR	M4PyOWlv\HWldHnvckBw\iCKRFHDMY1m\GmjdHXkJIhqe3SxbnWgZYNmfHmuYYTpc44>	NH[4WXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N\|g1OCd-MkCxOFM5PDB:L3G+
HepG2	M2O3TGtqdmG\|ZTDBd5NigQ>?	NITSUWQ2KM7:TR?=	NVLlbpg4Pmh?	M3n5TWROW09?	NVLxc5pUUW6mdXP0bY9vKG:oIFjERWMudWWmaXH0[YQhcGm\|dH;u[UBpgXCncnHj[ZR6dGG2aX;u	NFLzbmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N\|g1OCd-MkCxOFM5PDB:L3G+
HepG2	NFzER5NMcW6jc3WgRZN{[Xl?	NWPWVG56PSEQvF2=	NUfhR4tuPmh?	NG\Qc3BFVVOR	NF3QVmRKdmS3Y4Tpc44hd2ZiSFTBR{1u\WSrYYTl[EBidHCqYT30eYJ2dGmwIHHj[ZR6dGG2aX;u	M1PsbVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzPFQxLz5{MEG0N\|g1ODxxYU6=
HepG2	NHy0WXpMcW6jc3WgRZN{[Xl?	MmHxOUDPxE1?	MYC2bC=>	MVvEUXNQ	NH31eWhKdmS3Y4Tpc44hd2ZiSFTBR{1u\WSrYYTl[EBJOy:KNDDobZN1d26nIHHj[ZR6dGG2aX;u	M3f5dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzPFQxLz5{MEG0N\|g1ODxxYU6=
COLO205	MYfLbY5ie2ViQYPzZZk>	M{\nflExKM7:TR?=	M2\1OlI1KGh?	NUS0TppoTE2VTx?=	M{\1[WlvcGmkaYTpc44hd2ZiSFTBR\|Eh[XO\|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIhqe3SxbnWgTFMh[WOndInsZZRqd25id3n0bEBKSzVyIH;mJFIvOiEQvF2=	M2XBVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEWxN\|c5Lz5{MES1NVM4QDxxYU6=
SCC4	M3i0bWtqdmG\|ZTDBd5NigQ>?	M2frUlEh\|ryP	NWTJOHNVOjSq		NEOwVYRKdmirYnn0bY9vKG:oIFjERWMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6geJVjfWyrbjDhZ4V1gWyjdHnvci=>	MmjQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NUKyNlUoRjJyNEWyNlI2RC:jPh?=
SCC4	NHjyRZZMcW6jc3WgRZN{[Xl?	NHPiVZUyKM7:TR?=	MUiyOIg>		Mkf2TY5pcWKrdHnvckBw\iCKRFHDJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViYXPleJlt[XSrb36=	NEXQVmI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES1NlIzPSd-MkC0OVIzOjV:L3G+
SCC4	Ml;vSpVv[3Srb36gRZN{[Xl?	MljYNUDPxE1?	NIDsVHc2KG2rbh?=		MnnhRYdwdmm\|dDDhZ5Rqfmm2eTDheEBXTFJiYYPz[ZN{\WRiYYOgbY5lfWO2aX;uJI9nKEO\UEK0JIdmdmViZYjwdoV{e2mxbh?=	NXfXOJJrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC0OVIzOjVpPkKwOFUzOjJ3PD;hQi=>
NB4	Ml;6SpVv[3Srb36gRZN{[Xl?	MmjyOUDPxE1?	NF3ucVQzPCCq	NGmwXJVFVVOR	MnrSR4VtdCCleXPs[UBienKnc4SgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iYXPjeY12dGG2aX;uJIF1KHC{ZT3HNUBxcGG\|ZR?=	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR7MUS0NEc,OjB2OUG0OFA9N2F-
NB4	MYrLbY5ie2ViQYPzZZk>	M{fPSFUh\|ryP	NUHU[pB3OjRiaB?=	NULLbIN1TE2VTx?=	MXjJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO\|ZXSgZZMhcW6lcnXhd4UhcW5iYXPleJlt[XSnIIT1ZpVtcW5ibHX2[Yw>	M2Swb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEmxOFQxLz5{MES5NVQ1ODxxYU6=
NB4	MlHjSpVv[3Srb36gRZN{[Xl?	NGfGeYQ2KM7:TR?=	NFXVfVAzPCCq	NWjhN\|MyTE2VTx?=	NXfsNVMzUW6mdXP0bY9vKG:oIICyNXdCTjFxQ1nQNUBmgHC{ZYPzbY9v	NGPzeoI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES5NVQ1OCd-MkC0PVE1PDB:L3G+
NB4	NITFWYVMcW6jc3WgRZN{[Xl?	MmDNOUDPxE1?	NH;BXpIzPCCq	NYn3NWJ5TE2VTx?=	MlryTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W\|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViSEOgZYNmfHmuYYTpc44>	M{\OR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEmxOFQxLz5{MES5NVQ1ODxxYU6=
NUGC3	M{XxPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MVfHTVUxRTJwOUSg{txO	NYDPRoVCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4OVA6PzFpPkKwPFUxQTdzPD;hQi=>
A549	NXPx[HptTnWwY4Tpc44hSXO\|YYm=	MojQOUDPxE1?	MnrFN{Bp	M3zrfGROW09?	MkjuTY5lfWO2aX;uJI9nKGG3dH;wbIFogSCrbjDoeY1idiCDNUS5JINmdGy\|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEyFM1nJ	MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh3NUKwPEc,OjB6NUWyNFg9N2F-
HUT78	NVvPUWc5T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NFzGV2c4OiCq	NFnZbodFVVOR	MmPBS2k2OD1yLkOg{txO	MlHRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB6OESyNFgoRjJyOEi0NlA5RC:jPh?=
U937	NYXjUJRFT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NITRV5U4OiCq	NXPZOHdpTE2VTx?=	NVP5bo1IT0l3ME2wMlM6KM7:TR?=	MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh6NEKwPEc,OjB6OESyNFg9N2F-
A431	NUDyW25RS3m2b4TvfIlkKEG\|c3H5		M{TqUVczKGh?		M1zCVWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE1OzFiY3XscJMhd3[ncnX4dJJme3OrbnegSWdHWiC5aYToJGlEPTBib3[gOE4zKM7:TR?=	MofoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2NlkoRjJzMEiwOlI6RC:jPh?=
A549	MoDkR5l1d3SxeHnjJGF{e2G7		NXvNXolJPzJiaB?=		NIe5W5ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KG:4ZYLlfJBz\XO\|aX7nJGhFSUNid3n0bEBKSzVyIH;mJFEvQCEQvF2=	MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
CAL27	MnrZR5l1d3SxeHnjJGF{e2G7		M1;NRlczKGh?		NUHXbYM1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hS0GOMkegZ4VtdHNib4\ldoV5eHKnc4PpcochTUeIUjD3bZRpKEmFNUCgc4YhOy5{IN88US=>	NH7jcpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFYzQSd-MkGwPFA3Ojl:L3G+
HeLa	MXTDfZRwfG:6aXOgRZN{[Xl?		Mo\YO\|IhcA>?		M1vkOGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMhd3[ncnX4dJJme3OrbnegTGRCSyC5aYToJGlEPTBib3[gNU42KM7:TR?=	MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
Sf21	M2TJPGtqdmG\|ZTDBd5NigQ>?		MYCzJIg>		NYP6[IdxUW6qaXLpeIlwdiCxZjDGcIFoKHSjZ3fl[EBpfW2jbjDy[YNwdWKrbnHueEBJTEGFODDlfJBz\XO\|ZXSge4l1cCCLQ{WwJI9nKDFwMjFOwG0>	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
SKBR3	MlLuR5l1d3SxeHnjJGF{e2G7		MmDxO\|IhcA>?		MkHzR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2tDWjNiY3XscJMhd3[ncnX4dJJme3OrbnegTGVTOiC5aYToJGlEPTBib3[gNk43KM7:TR?=	NF7a[IY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFYzQSd-MkGwPFA3Ojl:L3G+
SKOV3	Ml7UR5l1d3SxeHnjJGF{e2G7		MYK3NkBp		MUXDfZRwfG:6aXPpeJkh[WejaX7zeEBpfW2jbjDTT29XOyClZXzsd{BwfmW{ZYjwdoV{e2mwZzDISXIzKHerdHigTWM2OCCxZjCyMlIh\|ryP	NUjNc2NqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3OjlpPkKxNFgxPjJ7PD;hQi=>
COR-L23	MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXWxNEDPxE1?	MnXxO\|IhcA>?	MkD0SG1UVw>?	NF\jV\|VIUTVyPUGuNUDPxE1?	NFOwOVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFY1Pyd-MkGwPFA3PDd:L3G+
DU145	NFXRc\|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NYrweJQ2OTBizszN	M{n2[lczKGh?	MYfEUXNQ	NH3qV49IUTVyPUGuNFUh\|ryP	M3;TSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEiwOlQ4Lz5{MUC4NFY1PzxxYU6=
LoVo	Mlr2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MmjFNVAh\|ryP	MYG3NkBp	MX3EUXNQ	NETLUFlIUTVyPUGuO{DPxE1?	MmnJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2OFcoRjJzMEiwOlQ4RC:jPh?=
MCR5-SV2	Mor4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NEXBflEyOCEQvF2=	NXf1VYRIPzJiaB?=	MXnEUXNQ	M{HzZ2dKPTB;Mj6xJO69VQ>?	NXrXNnBRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3PDdpPkKxNFgxPjR5PD;hQi=>
HCT116	NInvRWlMcW6jc3WgRZN{[Xl?	NHf3VJc{OCEQvF2=	NHTlNZg{OCCq	NWLFUVhwTE2VTx?=	NV60e2Q3UW6qaXLpeIlwdiCxZjDISGFEPiCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4Yh[WyyaHGteJVjfWyrbjDhZ4V1gWyjdHnvci=>	M2PDR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEiwOlQ4Lz5{MUC4NFY1PzxxYU6=
HCT116	MXjLbY5ie2ViQYPzZZk>	M4C3XlMxKM7:TR?=	MoHDN\|AhcA>?	MoO2SG1UVw>?	MX3Jcohq[mm2aX;uJI9nKEiGQVOxJIF{e2W\|c3XkJIF{KGmwZIXjeIlwdiCxZjDobZN1d26nIFizT\|kh[WOndInsZZRqd25?	NVjHRYVkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3PDdpPkKxNFgxPjR5PD;hQi=>
U937	MkizT4lv[XOnIFHzd4F6	NGP4T5Y2KM7:TR?=	M{DIVVI1KGh?	MnvUSG1UVw>?	M322[2lvcGmkaYTpc44hd2ZiaHnzeI9v\SCjY3X0fYx1emGwc3\ldoF{\SCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDv[kBi[2W2eXzheIlwdiCxZjDobZN1d26nIFizJIF1KEt7	MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7MkS5Nkc,OjF{OUK0PVI9N2F-
U937	NHrNS2FMcW6jc3WgRZN{[Xl?	NXLye4hiPSEQvF2=	MXqyOEBp	M{\1cGROW09?	M1Hm[2lvcGmkaYTpc44hd2ZiaHnzeI9v\SCjY3X0fYx1emGwc3\ldoF{\SCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDv[kBi[2W2eXzheIlwdiCxZjDobZN1d26nIFizJIF1KEtzOB?=	Ml7XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OUK0PVIoRjJzMkmyOFkzRC:jPh?=
U937	NWPqUmRiU2mwYYPlJGF{e2G7	NXm2U25TPSEQvF2=	NFrIS5IzPCCq	Mn\KSG1UVw>?	MkLLTY5pcWKrdHnvckBw\iCqaYP0c45mKGGlZYT5cJRz[W6\|ZnXyZZNmKGG\|c3Xzd4VlKGG\|IHnuZ5Jm[XOnIH;mJJBidi2jY3X0fYxifGWmIHjpd5RwdmViSESgcIV3\Wx?	MmHjQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OUK0PVIoRjJzMkmyOFkzRC:jPh?=
U937	MofZT4lv[XOnIFHzd4F6	NFjmW4E2KM7:TR?=	MWGxOUBucW5?	M4XHWWROW09?	M{nwS2lvcGmkaYTpc44hd2ZiaHnzeI9v\SCjY3X0fYx1emGwc3\ldoF{\SCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDv[kBpcXO2b37lJGg{KGGlZYT5cIF1cW:wIHzleoVt	NIPQcYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUK5NlQ6Oid-MkGyPVI1QTJ:L3G+
A2780	NF;DdlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NVnVPYhuQTZiaB?=	MnrCSG1UVw>?	NV;1eW5kUUN3ME2xMlYzKM7:TR?=	NVL1UIxvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG2N\|Q1OzBpPkKxOlM1PDNyPD;hQi=>
COLO205	M33m[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		MV:5OkBp	MkXFSG1UVw>?	MmfLTWM2OD1{LkGyJO69VQ>?	NXfxRmhrRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG2N\|Q1OzBpPkKxOlM1PDNyPD;hQi=>
Hep3B	M4Hwcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		M1TiU\|Q5KGh?	MoDaSG1UVw>?	MkXsS2k2OD1zLkKxJO69VQ>?	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMkG0Okc,OjF5MUKxOFY9N2F-
GM11272	MYHGeY5kfGmxbjDBd5NigQ>?	MWixNFAhdk1?	NXXVNGtmPzJiaB?=	MUnEUXNQ	M1rOS3Jm[WO2aY\heIlwdiCxZjDN[WNxOiCvdYThcpQh\XiycnXzd4lwdiCjdDCxNEB1dyBzMECgcm0>	M{XOPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOESwO\|E3Lz5{MUi0NFcyPjxxYU6=
CFBE41o	M4HHOmtqdmG\|ZTDBd5NigQ>?	MmCzOUDPxE1?		NFK1NnRFVVOR	NGW3TWVFd3ewIILl[5Vt[XSrb36gc4YhUESDQ{egZZQhOC5{IITvJFUhfU1?	M4e3XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUi0PVU5Lz5{MUm4OFk2QDxxYU6=
CFBE41o	NE\0W4VHfW6ldHnvckBCe3OjeR?=	NV;XTmhvOSEQvF2=	NUHTWphTOjRiaB?=	Mnn0SG1UVw>?	M{LHOXJme3SxcnH0bY9vKG:oIFPGWHIh\GWudHHGOVA5KG23dHHueEB1emGoZnnjb4lv\yC2bzDj[YxtKHO3cn\hZ4Uh[XNiZ3z5Z49nd3Kvcx?=	M{TEdVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzOUi0PVU5Lz5{MUm4OFk2QDxxYU6=
DU145	NIDubndMcW6jc3WgRZN{[Xl?	MorBNk42KM7:TR?=	NHzrZWQzPCCq	M{DVU2ROW09?	NXribFBIUW6qaXLpeIlwdiCxZjDISGFEKGG\|c3Xzd4VlKGG\|IIXwdoVofWyjdHnvckBw\iCyMkH3ZYYyKHC{b4TlbY4h\XiycnXzd4lwdg>?	Ml;pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{NkCxOlYoRjJ{Mk[wNVY3RC:jPh?=
DU145	NX7SNHRCU2mwYYPlJGF{e2G7	NWnsbmlCPSEQvF2=	NFG2bZMzPCCq	MVHEUXNQ	NHX4RlBKdmirYnn0bY9vKG:oIFjERWMh[XO\|ZYPz[YQh[XNiaHnzeI9v\SCKNDDhZ4V1gWyjdHnvci=>	MljKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{NkCxOlYoRjJ{Mk[wNVY3RC:jPh?=
DU145	MljKT4lv[XOnIFHzd4F6	NYP5NmRNPSEQvF2=	NILMPIQzPCCq	Mk\YSG1UVw>?	NYTKboVvUW6qaXLpeIlwdiCxZjDISGFEKGG\|c3Xzd4VlKGG\|IHjpd5RwdmViSEOgbJlx\XKjY3X0fYxifGmxbh?=	NYSzWlZVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlAyPjZpPkKyNlYxOTZ4PD;hQi=>
786-0	NETRc29Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NITjWFM3NjJizszN	M4Kwd\|czKGh?	M2\MRWROW09?	NV7a[HNzT0l3ME2zMlIh\|ryP	MlvVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
A498	M{LLNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MYq2MlIh\|ryP	MlrXO\|IhcA>?	NVThOJc1TE2VTx?=	MXPHTVUxRTFwNjFOwG0>	MXm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
A549/ATCC	Mm\sS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	Mnv4Ok4zKM7:TR?=	NVjnZ4pyPzJiaB?=	M4XR[GROW09?	MUnHTVUxRTFwNjFOwG0>	Ml3iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
ACHN	NFTpVXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MYi2MlIh\|ryP	NXjub49lPzJiaB?=	M2i4OWROW09?	Mln0S2k2OD1zLkOg{txO	NWOy[IZGRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
BT549	Mln0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M2DLbFYvOiEQvF2=	MYi3NkBp	Mn\mSG1UVw>?	M1rYeGdKPTB;MT6zJO69VQ>?	NEnVeIE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
EKVX	NV[5eYVPT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M2HhTlYvOiEQvF2=	M4HhblczKGh?	M3;FbWROW09?	MoPDS2k2OD1zLkOg{txO	MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
HCT15	MnOwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MoO5Ok4zKM7:TR?=	MmXvO\|IhcA>?	MWjEUXNQ	NXi4TXlNT0l3ME2yMlUh\|ryP	MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
HOP92	M3v4Xmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NE\PdXU3NjJizszN	M{fSSlczKGh?	MUXEUXNQ	MULHTVUxRTRizszN	M4WwWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
IGROV1	NULme5BLT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NHfBUoM3NjJizszN	M1XxN\|czKGh?	NFvhfFZFVVOR	NYPDTGJvT0l3ME2xMlMh\|ryP	NX\MSWhqRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
LOXIMVI	MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	Mn;pOk4zKM7:TR?=	NInnUms4OiCq	M3TzSWROW09?	NFS2fmlIUTVyPUGg{txO	M1W2XlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
M14	NH3MOnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NFn0PGY3NjJizszN	M2PGRVczKGh?	MWfEUXNQ	NWL1b3R1T0l3ME2xMlMh\|ryP	M1PQflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
MALME-3M	NFHGZXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	Mm\UOk4zKM7:TR?=	M4\zdFczKGh?	MVrEUXNQ	NIXCc3RIUTVyPUCuN\|Ih\|ryP	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
MCF10A	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NXv0dGxqPi5{IN88US=>	NETFRnQ1QCCq	NHP0T4pFVVOR	MmX2S2k2OD17Lk[g{txO	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
MDA-MB-435	MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NEflc5E3NjJizszN	NED0PFY4OiCq	MU\EUXNQ	NYL3eZhLT0l3ME2wMlUh\|ryP	NVmzdo5NRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
MDA-MB-468	M4Locmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MonnOk4zKM7:TR?=	MorFO\|IhcA>?	MVLEUXNQ	NILGVoRIUTVyPUCuOlMh\|ryP	NY\ZTos1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
MOLT4	NEDFV\|BIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MVy2MlIh\|ryP	MWC3NkBp	MV;EUXNQ	NYPMNGh6T0l3ME2wMlQh\|ryP	NHPNWmE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
NCI-ADR-RES	NYnkcoRHT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	M13GPFYvOiEQvF2=	NH7T[2s4OiCq	M3PEVGROW09?	MkPyS2k2OD1yLkG2JO69VQ>?	NGn4fGw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
NCI-H23	MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXi2MlIh\|ryP	NVLmT4w{PzJiaB?=	M4HtVGROW09?	MoWyS2k2OD1zIN88US=>	M13DSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
NCI-H322M	MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MWC2MlIh\|ryP	NVP2PIxtPzJiaB?=	MXjEUXNQ	MWDHTVUxRTBwN{mg{txO	MXW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
NCI-H460	MnfMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	M1[2UFYvOiEQvF2=	MUe3NkBp	NFLpeXVFVVOR	MYrHTVUxRTBwN{mg{txO	MUC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
NCI-H522	NWLsOlRvT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MVm2MlIh\|ryP	NEm3fHE4OiCq	Mlv0SG1UVw>?	NICxU3NIUTVyPUCuOUDPxE1?	MkfoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
OVCAR3	NIPaNWxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	NFi2Xpc3NjJizszN	MYG3NkBp	NHfKNZBFVVOR	NILuRVVIUTVyPUGuN{DPxE1?	NUjn[JVNRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
OVCAR4	NWnoXlk1T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MoXPOk4zKM7:TR?=	NYHzNoliPzJiaB?=	M37kSWROW09?	MmjoS2k2OD12IN88US=>	NHfKZVk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
OVCAR5	MmfES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NH3KOIk3NjJizszN	NF23d4E4OiCq	NWP6TJhTTE2VTx?=	NHfJd5BIUTVyPUCuO\|kh\|ryP	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
OVCAR8	MnraS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NHfSPZY3NjJizszN	Ml\lO\|IhcA>?	NWTwbHROTE2VTx?=	NY\EUFRPT0l3ME2wMlUh\|ryP	NXzEfpFRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
RXF393	MmTHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NWT0[VY2Pi5{IN88US=>	MnLoO\|IhcA>?	MYjEUXNQ	NGq5XVNIUTVyPUGuN{DPxE1?	MlHRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
SF268	MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NXr5S\|JkPi5{IN88US=>	MVu3NkBp	NV;YT5JQTE2VTx?=	M{jL[2dKPTB;MT62JO69VQ>?	MkLuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
SF295	MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NUDrUWNRPi5{IN88US=>	NVfKPVZGPzJiaB?=	M{ftfWROW09?	NUXNSnhKT0l3ME2xMlYh\|ryP	M{K5SFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
SF539	MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MnPDOk4zKM7:TR?=	M1XiTVczKGh?	M13KdGROW09?	NFP5dWNIUTVyPUKg{txO	MmLuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
SK-MEL-2	M4jpS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	M1rpU\|YvOiEQvF2=	NVHpTGtLPzJiaB?=	Ml[4SG1UVw>?	MVLHTVUxRTFwMzFOwG0>	NWDO[IVpRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
SK-MEL-28	M3\rXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NH\h[IQ3NjJizszN	NYX0UmwyPzJiaB?=	NXrRNWhOTE2VTx?=	NEnFemNIUTVyPUGg{txO	MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
SK-MEL-5	MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NIfhN\|Q3NjJizszN	NUHVZYN3PzJiaB?=	MV\EUXNQ	MUHHTVUxRTBwNkOg{txO	MXe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
SKOV3	MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MoixOk4zKM7:TR?=	MVK3NkBp	NUHkcI9GTE2VTx?=	NU\SZVZWT0l3ME2xJO69VQ>?	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
SN12C	NYDkOnF{T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	MVu2MlIh\|ryP	MUK3NkBp	MUTEUXNQ	M{nJWWdKPTB;MjFOwG0>	NYXaR3doRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
SNB19	MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NH7DOFI3NjJizszN	NYTvfo9IPzJiaB?=	MnPLSG1UVw>?	MXnHTVUxRTBwNkOg{txO	MX28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
SNB75	MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MXy2MlIh\|ryP	NF2zTHc4OiCq	NHztTnVFVVOR	MWfHTVUxRTBwN{mg{txO	M3TJfVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
SR	MlrWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MmDuOk4zKM7:TR?=	M1fUTlczKGh?	MkL3SG1UVw>?	NI\IfnRIUTVyPUCuOEDPxE1?	MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR\|NU[2PUc,OjJ2M{W2Olk9N2F-
T47D	M{XOXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	MlfhOk4zKM7:TR?=	M3zy[VczKGh?	M{DUemROW09?	Mn74S2k2OD1yLkWg{txO	NIHqSFk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
TK10	M1;SXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NUnE[25mPi5{IN88US=>	NYLQNm1XPzJiaB?=	NGLjOHRFVVOR	MkjCS2k2OD1yLk[zJO69VQ>?	NF;tclk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N\|U3Pjl:L3G+
UACC257	NYO5fnNMT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=	NX7tOXA{Pi5{IN88US=>	M{jUUVczKGh?	MYLEUXNQ	NWHE[Gw{T0l3ME2wMlUh\|ryP	MoH4QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
UACC62	MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	NFXPXFk3NjJizszN	NYmwOYh2PzJiaB?=	M4HKSGROW09?	MlLDS2k2OD1yLkSg{txO	NYDhUZp1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N\|U3PjlpPkKyOFM2PjZ7PD;hQi=>
UO31	MlrJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	NX7LSXRFPi5{IN88US=>	NFfjXFc4OiCq	MXTEUXNQ	NXnjPIFKT0l3ME2wMlUh\|ryP	MkHzQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
Hep3B	MnHXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		NEnWUpo1QCCq	M1\IZmROW09?	MWnHTVUxRTBwNkmg{txO	NFrrbHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzPVg3Oyd-MkK0N\|k5PjN:L3G+
BE(2)-C	NXjN[nhiT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		MnX5O\|IhcA>?	M2nM[mROW09?	Mnm4TWM4OD1{IN88US=>	Mmq2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ7M{KzNVYoRjJ{OUOyN\|E3RC:jPh?=
Bel7402	MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		MlzYO\|IhcA>?	M1XKcWROW09?	NGe3ZXFIUTlyPUG3JO69VQ>?	M3HPXVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|ME[xN\|c3Lz5{M{C2NVM4PjxxYU6=
Bel7404	MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		M4T0S\|czKGh?	MUXEUXNQ	MVHHTVkxRTF6IN88US=>	MXO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MUO3Okc,OjNyNkGzO\|Y9N2F-
BGC823	NYnoVm9VT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		Mm\SO\|IhcA>?	MoXVSG1UVw>?	M1W1O2dKQTB;Nz61JO69VQ>?	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MUO3Okc,OjNyNkGzO\|Y9N2F-
MGC803	NUHlSo5XT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NYrYR\|BUPzJiaB?=	MnXOSG1UVw>?	MYDHTVkxRTFyLkig{txO	MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MUO3Okc,OjNyNkGzO\|Y9N2F-
SGC7901	NF:x[VZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		M1rROlczKGh?	MWnEUXNQ	MV3HTVkxRTlwNDFOwG0>	NGHjXpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
SKHEP1	NGqyN\|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		MlvlO\|IhcA>?	NUn2NFI6TE2VTx?=	MnLMS2k6OD1zNzFOwG0>	NETROm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
SMMC7721	MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NEH1cnQ4OiCq	NULLZYFJTE2VTx?=	NUTKUnF1T0l7ME2yNEDPxE1?	NEG5PYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
NUGC3	MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?	MWSxNEDPxE1?			NEH4ZVBIUT1{Lkm0JO69VQ>?	NW\s[XRiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlM{OzJpPkKzNVY{OzN{PD;hQi=>
A2780 CisR	MmnYT4lv[XOnIFHzd4F6		NInUWmYyQCCq		NUX6TJI{UW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlQ1KM7:TR?=	M{Drb\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
CAL27 sens	MUnLbY5ie2ViQYPzZZk>		MoXONVghcA>?		M175bmlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD6zOkDPxE1?	MkLqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ\|MkWyOlA{RC:jPh?=
CAL27 CisR	MUfLbY5ie2ViQYPzZZk>		NXL1SoVlOThiaB?=		Mne2TY5pcWKrdHnvckBw\iCKRFHDJJdqfGhiSVO1NEBw\iByLk[xJO69VQ>?	M1vpPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
KYSE-510 CisR	MWLLbY5ie2ViQYPzZZk>		NEW0c\|MyQCCq		NWDufHZlUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlU6KM7:TR?=	NUHDbo1nRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB\|PD;hQi=>
KYSE-510 sens	MnLGT4lv[XOnIFHzd4F6		M2nRc\|E5KGh?		MXfJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNzFOwG0>	M1y4W\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
MDA-MB-231 sens	NXv1NpBmU2mwYYPlJGF{e2G7		NXroN5JrOThiaB?=		M3KwWmlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD62NUDPxE1?	NUi0cnFFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB\|PD;hQi=>
MDA-MB-231 CisR	MXfLbY5ie2ViQYPzZZk>		M1XPXFE5KGh?		MWjJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNkGg{txO	M4TNd\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
ES2	NV6xXGpnT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=		NXvvW44yPDhiaB?=	MmnDSG1UVw>?	Mk[2TWM2OD1zMj63JO69VQ>?	NULYW2o1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVM1PDlpPkKzOFk{PDR7PD;hQi=>
MDA-MB-231	MoS4T4lv[XOnIFHzd4F6	NFvQeXEyOCEQvF2=	MWWzNEBucW5?	MoTsSG1UVw>?	NYLCRlJlUW6qaXLpeIlwdiCxZjDISGFEOmF?	NX7lVY91RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVM1PDlpPkKzOFk{PDR7PD;hQi=>
MDA-MB-231	M13kTmtqdmG\|ZTDBd5NigQ>?	NHnBRpIyKM7:TR?=	M4jEUFI1KGh?	NHi5Z3ZFVVOR	MnPGTY5pcWKrdHnvckBw\iCKRFHDOkBie3Onc4Pl[EBieyCjY3X0fYxifGmxbjDs[ZZmdHNib3[geJVjfWyrbh?=	NXO4TVQyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVM1PDlpPkKzOFk{PDR7PD;hQi=>
HS68	M3n0O2N6fG:2b4jpZ{BCe3OjeR?=	NEX1[4UyOCEQvF2=	M2DTW\|czKGh?	MWrEUXNQ	Ml[1TWM2OD12Lk[g{txO	M1G5U\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NUewOVQzLz5{M{W3NFU1OjxxYU6=
MEF	MVXDfZRwfG:6aXOgRZN{[Xl?	NXjHcIlYOTBizszN	MV[3NkBp	Mni3SG1UVw>?	NH;OZY1KSzVyPUSuOEDPxE1?	M3zsbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NUewOVQzLz5{M{W3NFU1OjxxYU6=
BGC823	NICzW\|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			M4LMOGROW09?	MWDHTVUxRTNwMEmg{txO	Mkn2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4MEG3NFYoRjJ\|NkCxO\|A3RC:jPh?=
High5	M4nqS2tqdmG\|ZTDBd5NigQ>?		MnHUNlQhcA>?		MWfJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVO2JJdqfGhiSVO1NEBw\iByLkC5JO69VQ>?	M1\jPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NkCyOVI{Lz5{M{[wNlUzOzxxYU6=
High5	M1uxXmtqdmG\|ZTDBd5NigQ>?		MXSyOEBp		NYXqPVFPUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEOSC5aYToJGlEPTBib3[gNE4yOiEQvF2=	MkPwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4MEK1NlMoRjJ\|NkCyOVI{RC:jPh?=
High5	M3fhRWtqdmG\|ZTDBd5NigQ>?		NYfLUJE1OjRiaB?=		MkfVTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDN{B4cXSqIFnDOVAhd2ZiMD6xO{DPxE1?	MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZyMkWyN{c,OjN4MEK1NlM9N2F-
A549	NV3BT\|lFT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=			NV;EcWVHTE2VTx?=	MlvoTWM2OD1zLkC3PUDPxE1?	M2r4TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|NkS0NlExLz5{M{[0OFIyODxxYU6=
HCT116	NIKyOIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=			MVfEUXNQ	NE\ZdFZKSzVyPUCuO\|U4KM7:TR?=	NXrtN2dVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2OFQzOTBpPkKzOlQ1OjFyPD;hQi=>
K562	MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?			MVTEUXNQ	MmjUTWM2OD1yLk[0OUDPxE1?	MmPRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NESyNVAoRjJ\|NkS0NlExRC:jPh?=
GBC-SD	MlzES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?	MW[1JO69VQ>?	Ml;aO\|IhcA>?	NY\QN3dHTE2VTx?=	MkC5TWM2OD1zND64JO69VQ>?	NYrFXIx4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFczPjBpPkKzO\|A4OjZyPD;hQi=>
HGC27	NIjRd2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=	MnKxOUDPxE1?	M3L1PFczKGh?	M{TQcmROW09?	MWrJR\|UxRTVwNDFOwG0>	NYrGRohxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFczPjBpPkKzO\|A4OjZyPD;hQi=>
HL60	MmW2T4lv[XOnIFHzd4F6	MmXSOUDPxE1?	MXKyOEBp	Mme4SG1UVw>?	NGL4NYJKdmirYnn0bY9vKG:oIFjERWM>	M3jLc\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ\|N{C3NlYxLz5{M{ewO\|I3ODxxYU6=
Vero	MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		M17xblczKGh?	NHHQclBFVVOR	M{O0V2lEPTB;MD65JO69VQ>?	NH33bVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e4OlQ2Oid-MkO3PFY1PTJ:L3G+
NCI60	NFrjfopEgXSxdH;4bYMhSXO\|YYm=		NGfnfVM1QCCq	M2j2TWROW09?	NFrEdm5IUT1yLkWzJO69VQ>?	NY\2TnBFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNVU{OjdpPkK0NFE2OzJ5PD;hQi=>
SW620	MUnDfZRwfG:6aXOgRZN{[Xl?		MnXJOFghcA>?	M172fmROW09?	NHfsVXRIUT1yLkW0JO69VQ>?	M3XWVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEG1N\|I4Lz5{NECxOVMzPzxxYU6=
U251	NVrxOYhrS3m2b4TvfIlkKEG\|c3H5		NUHDWJZmPDhiaB?=	MV7EUXNQ	Mn;HS2k:OS53MzFOwG0>	NIHyb\|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECxOVMzPyd-MkSwNVU{Ojd:L3G+
KM12	MUDDfZRwfG:6aXOgRZN{[Xl?		NF7kRpY1QCCq	MlvOSG1UVw>?	NWm5T2JTT0l;MT64PEDPxE1?	MoCyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUWzNlcoRjJ2MEG1N\|I4RC:jPh?=
Hs578T	MnHCR5l1d3SxeHnjJGF{e2G7		NXni[4lWPDhiaB?=	NIDKWGNFVVOR	NX7xbHFXT0l;ND64N{DPxE1?	MmjxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUWzNlcoRjJ2MEG1N\|I4RC:jPh?=
MDA-MB-231	M4TwTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		M1jhdlQ5KGh?		MUTJR\|UxRTBwMU[g{txO	M4LOS\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEm1NFE3Lz5{NEC5OVAyPjxxYU6=
MCF7	MnHYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		MlX5OFghcA>?		NXe4dVhMT0l;MD60OUDPxE1?	M2DDeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUG5OVU2Lz5{NEGxPVU2PTxxYU6=
MDA-MB-435	M{nkdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7		NF3WVGg1QCCq		MnnuS2k:OC56ODFOwG0>	NUPy[mhHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNVk2PTVpPkK0NVE6PTV3PD;hQi=>
AsPC1	M1XyU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7	NGPoZoE{OCEQvF2=	NWHz[ZJkPDhiaB?=	MUTEUXNQ	NUHlSIZQUUN3ME2zMlY3KM7:TR?=	NETKRpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG4OVM4QCd-MkSxPFU{Pzh:L3G+
Jurkat E6.1	MYrLbY5ie2ViQYPzZZk>		MlK0NkBp	NFOwN\|ZFVVOR	M2ryRWlvcGmkaYTpc44hd2ZiSFTBR\|Qhf2m2aDDJR\|UxKG:oIECuNFQh\|ryP	MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ4MUi2Nkc,OjR{NkG4OlI9N2F-
KG1	MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		Mnr4OFghcA>?		NFXRUlFKSzVyPUGuNVUh\|ryP	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV{NUCwN{c,OjR3MkWwNFM9N2F-
HEL	MkfmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?		NV:zWnNvPDhiaB?=	NXj5RmFMTE2VTx?=	NG\CWZBKSzVyPUCuOFkh\|ryP	NUHVZZVPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2PVQxPTVpPkK0Olk1ODV3PD;hQi=>
HSC3	M36we2N6fG:2b4jpZ{BCe3OjeR?=		NIP2PJUyOiCq	M2XNfmROW09?	MXTJR\|UxRTBwN{Gg{txO	M4jENlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{[2OVYxLz5{NEe2OlU3ODxxYU6=
HT-29	Ml3jR5l1d3SxeHnjJGF{e2G7		Mne3NVIhcA>?	NFHT[nhFVVOR	NYK1[HR[UUN3ME2wMlczKM7:TR?=	NGjBPJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2OlU3OCd-MkS3OlY2PjB:L3G+
KB	MkDsR5l1d3SxeHnjJGF{e2G7		MmDNNVIhcA>?	NELYeZBFVVOR	NXzGU4FPUUN3ME2wMlc2KM7:TR?=	MlO5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5Nk[1OlAoRjJ2N{[2OVYxRC:jPh?=
H460	Mlf2R5l1d3SxeHnjJGF{e2G7		M3rUblEzKGh?	MmLRSG1UVw>?	MmrzTWM2OD1yLkiyJO69VQ>?	NXjOeYpZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OlY2PjBpPkK0O\|Y3PTZyPD;hQi=>
MONE1	NWfQfIpjS3m2b4TvfIlkKEG\|c3H5		NV7jWmNoOTJiaB?=	NX\P[G5CTE2VTx?=	MXXJR\|UxRTFwMUig{txO	MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4NkW2NEc,OjR5Nk[1OlA9N2F-
MKN45	NWjwfYNIS3m2b4TvfIlkKEG\|c3H5		NUXoUVB1OTJiaB?=	MmWwSG1UVw>?	MkTZTWM2OD1zLkK1JO69VQ>?	M37rN\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{[2OVYxLz5{NEe2OlU3ODxxYU6=
TSGH	NFf2d4VEgXSxdH;4bYMhSXO\|YYm=		NX61RnJEOTJiaB?=	MkK0SG1UVw>?	MYXJR\|UxRTJwMEmg{txO	NGn3UJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2OlU3OCd-MkS3OlY2PjB:L3G+
HL60	NI[2boJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=		NYDNb\|VIPzJiaB?=	MUnEUXNQ	NIj5cnlIUTVyPUCuPVMh\|ryP	NHrpSGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFUxOCd-MkS5NFA2ODB:L3G+
HEK293	MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?		NW\mfmpEPzJiaB?=	NFj5WllFVVOR	MUTHTVUxRTF\|IN88US=>	NVHIWIRDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA2ODBpPkK0PVAxPTByPD;hQi=>
Jurkat	M3z2N2N6fG:2b4jpZ{BCe3OjeR?=	M3;CXVExOCEQvF2=	NF3NO\|c4OiCq		M4nkVGdKPTB;MD65JO69VQ>?	NI\BfYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFc1Oyd-MkS5NFA4PDN:L3G+
GM15850	NEXUbplMcW6jc3WgRZN{[Xl?	Mo[3Nk42KM7:TR?=	MU[5OkBp	NXq5XFJ6TE2VTx?=	MVrkc4V{KG6xdDDpcohq[mm2IFjERWMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIN{BtgXOrbnWgNVQh[WOndInsZZRqd25ib3[gSnhPKGenbnW=	Mmn6QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7MkGzOlcoRjF4OUKxN\|Y4RC:jPh?=
GM15850	MlX0T4lv[XOnIFHzd4F6	MYeyMlUh\|ryP	M2\6NFk3KGh?	NHfacZNFVVOR	NG\yUHNld2W\|IH7veEBqdmirYnn0JGhFSUNiYYPz[ZN{\WRiYYOgbY5kemWjc3WgbY4hcGm\|dH;u[UBJPCCueYPpcoUhPSCjY3X0fYxifGmxbjDv[kBHYE5iZ3Xu[S=>	MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjl{MUO2O{c,OTZ7MkGzOlc9N2F-
GM15850	MoHoT4lv[XOnIFHzd4F6	MX:yMlUh\|ryP	MnPqPVYhcA>?	NHi1SY5FVVOR	MW\kc4V{KG6xdDDpcohq[mm2IFjERWMh[XO\|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIOEBtgXOrbnWgNVIh[WOndInsZZRqd25ib3[gSnhPKGenbnW=	M1LldVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUKxN\|Y4Lz5zNkmyNVM3PzxxYU6=
GM15850	MmLNT4lv[XOnIFHzd4F6	MUGyMlUh\|ryP	NVW0Toh3QTZiaB?=	NXXxWW9lTE2VTx?=	M2e1dIRw\XNibn;0JIlvcGmkaYSgTGRCSyCjc4Pld5Nm\CCjczDpcoNz\WG\|ZTDpckBpcXO2b37lJGg{KGy7c3nu[UA6KGGlZYT5cIF1cW:wIH;mJGZZViCpZX7l	M{fZblxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUKxN\|Y4Lz5zNkmyNVM3PzxxYU6=
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CTB-1	NHfmXJdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MUTJR\|UxRTV\|Nz65NkBvVQ>?	NVLtR4djRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
HD-MY-Z	M1OzR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				M1;NRmlEPTB;NUO4MlE{KG6P	MUK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
NKM-1	M2PQb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				Mk\uTWM2OD13NESuNlchdk1?	M33NZVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
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LU-135	NXnyN\|E2T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MnyxTWM2OD1zOT6yOlM3KM7:TR?=	Ml;JQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NCI-H2029	MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NFKyXXFKSzVyPUG5MlYxOjFizszN	NHjt[og9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
HT-1197	MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NGiwVFhKSzVyPUG5MlkzOjdizszN	NEfpRng9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
IGR-1	MnjUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NHvRWYtKSzVyPUKwMlMyOTdizszN	M4\OcFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
U-87-MG	MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M1LyWGlEPTB;MkCuOlQyOyEQvF2=	MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
NCI-H1304	M1LHVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MmPjTWM2OD1{MD63OVAyKM7:TR?=	NYTaTWlURGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
RCC10RGB	MkTjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				MV;JR\|UxRTJzLkK4Nlkh\|ryP	M3vyflxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H520	M{L1VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MXXJR\|UxRTJ{Lke2NVEh\|ryP	M3\VeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
SK-LU-1	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NGnSWnpKSzVyPUKzMlA4OThizszN	NF3kbZk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
UACC-893	NYXmRWxyT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NEW3S2xKSzVyPUKzMlExOzhizszN	M{f5S\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H2347	NFP3[pJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NUPGUohxUUN3ME2yN{4zPjd2IN88US=>	Ml7nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
U-118-MG	MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				MVzJR\|UxRTJ\|LkeyNVch\|ryP	M4nxW\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H596	NGTiRpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MYrJR\|UxRTJ\|LkmwOVUh\|ryP	MlfuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
MZ1-PC	NXzKV2pmT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				M3jGWWlEPTB;MkSuOlc2QSEQvF2=	NY[w[WFwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
SW1417	NYjBb284T3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NUO1SmQ4UUN3ME2yOU4zPDl6IN88US=>	MkLEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NCI-H82	MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NFOxSm9KSzVyPUK2MlQzQDhizszN	NXT6dVdxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
COLO-678	NEjofJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MmD2TWM2OD1{Nz60PVE6KM7:TR?=	Mn30QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
KURAMOCHI	Mki3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M{HXRmlEPTB;MkiuNVI3KM7:TR?=	M1jK[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H1650	MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NFf2PJJKSzVyPUOwMlE6OjRizszN	M2XRUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
HCC1954	NEX1RVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NVfORXMyUUN3ME2zNU4{PjZ4IN88US=>	NWTMUlY5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
GAK	MnL1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				Ml;YTWM2OD1\|Mj6yOlE3KM7:TR?=	MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
YAPC	NH7TdG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MmPDTWM2OD1\|Mz63OlQ5KM7:TR?=	NFnmOW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NCI-H2228	MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				NVLFflF2UUN3ME2zOE4{PjB3IN88US=>	NEnQWoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
VMRC-RCZ	NIjxNGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				MWLJR\|UxRTN4LkOwNlIh\|ryP	MmDTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NMC-G1	M3Pw[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7				NHj0c25KSzVyPUO3MlA2OjlizszN	NIToNYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
D-336MG	M2jS[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7				MYXJR\|UxRTRzLki0OFIh\|ryP	MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
UACC-62	MmPXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				M3HidmlEPTB;NEKuOVUxPSEQvF2=	NHTSdG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
C-33-A	MoCzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NXPNcIl6UUN3ME20OU4{OjV4IN88US=>	NGHOcWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
HCC1937	MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>?				M4HURmlEPTB;NEeuNVk2QCEQvF2=	NH;TcXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
HDLM-2	NWHGWFkzT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				NHrHeHpKSzVyPUS3MlIzQDdizszN	M3rkR\|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H727	MoXES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl?				NG\sW4RKSzVyPUS3MlI1PTFizszN	MkHPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NY	NFvtVXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?=				NUPJZVFQUUN3ME20PU4{QTB6IN88US=>	NGLW[Y49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NCI-H2291	NVy4d3pWT3Kxd4ToJGlvcGmkaYTpc44hSXO\|YYm=				MmroTWM2OD12OT64N\|AyKM7:TR?=	NYnrdlFWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC\|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+

... Click to View More Cell Line Experimental Data

Assay

Methods	Test Index	PMID
Western blot	Ac-Histone H4 / Ac-Histone H3; PubMed: 21598070 J Neurooncol D54 and U87 glioma cells were treated with lower concentrations (1-3 µM) of vorinostat. The effects on histone acetylation was assessed by immunoblotting. phospho-CDK1; PubMed: 21598070 Changes in level of inhibitory phosphorylation of cdk1 after treatment with vorinostat was determined at 24 and 48 h (using a Tyr-15 residue phospho-specific antibody) along with total cdk1 as loading control. phospho-Cdc25c / Cdc25c; PubMed: 21598070 J Neurooncol Time course of changes in phospho-cdc25c levels after exposure to vorinostat (5 µM). Cyclin B1; PubMed: 21598070 J Neurooncol Cyclin B1 levels were determined in D54 and U87 cells after treatment with various doses of vorinostat at 24 and 48 h. c-Myc / c-Raf / Akt / Cyclin D1 / CDK4; PubMed: 19440035 Cancer Biol Ther Cell lysates from JeKo-1 and Mo2058 cells exposed to vorinostat were immunoblotted for c-Myc, c-Raf, AKT, cyclin D1, CDK4 and acetylated histone H3. The levels of β-actin served as the loading control. p-eIF2α / ATF4 / CHOP ; PubMed: 20072656 Neoplasia Western blot was performed to evaluate activated eIF2α (P-eIF2α), translational up-regulation of ATF4, and CHOP expression in U251 cells after vorinostat exposure. p21; PubMed: 21598070 J Neurooncol To determine the dose response relationship to vorinostat, cells were exposed to various higher concentrations (5–10 µM) of the agent and the levels of p21 determined by immunoblotting.	21598070 19440035 20072656
Immunofluorescence	HDAC1 / CK19; PubMed: 29917299 Cancer Sci Immunocytochemistry to detect the expression of HDAC1 and CK 19 in PDAC cells with/without Vorinostat treatment. HDAC1 and CK19 were stained red and green, respectively. The Hoechst‐stained nuclei appear blue. Scale bar: 100 μm. The CK19 expression in PSN1 and MiaPaCa2 was increased by Vorinostat treatment. Nrf2; PubMed: 20566897 Blood Vorinostat induced translocation of Nrf2 from cytosol to nucleus. U937 cells were treated with 2μM vorinostat for 18 hours and then cytospun. Cells were fixed and labeled for Nrf2 protein (red) and nucleus (blue, 4,6-diamidino-2-phenylindole) as described in “Immunofluorescence and confocal microscopy.” Images were taken by Nikon Eclipse TE2000 confocal microscope with 40×/1.30 numeric aperture oil objective lens. Ctrl indicates control cells without treatment; and Vor, vorinostat. α-SMA; PubMed: 26330748 Mol Vis Representative immunofluorescence images and the quantification graph showing the effect of vorinostat (2.5 µM), TGIF1, and TGIF2 siRNA knockdown on αSMA. Control human corneal fibroblast cultures (-TGFβ) showed no αSMA staining. Cultures treated with TGFβ1 (5 ng/ml) showed a robust αSMA staining. Vorinostat treatment markedly attenuated TGFβ-evoked αSMA staining. The knockdown of TGIF1 (+TGFβ+vorinostat+TGIF1siRNA) and TGIF2 alone (+TGFβ+vorinostat+TGIF2siRNA) or in combination (+TGFβ +vorinostat +TGIF1 +2siRNA) markedly blocked the effect of vorinostat on αSMA. Scale bar=100 μm. Ac-STAT3 / IGF2; PubMed: 27086926 Oncogene Immunofluorescence staining of Ac-STAT3 and IGF2 after vorinostat treatment.	29917299 20566897 26330748 27086926
Growth inhibition assay	Cell apoptosis; PubMed: 19440035 Cancer Biol Ther Cells were treated with indicated concentrations of vorinostat for 48 hours. Following this, the percentage of annexin-V-stained apoptotic cells was determined by flow cytometry. Values represented as bar graphs are the mean of 3 experiments plus or minus the standard error (SE) Cell viability; PubMed: 27086926 Oncogene The effects of vorinostat on the viability of the indicated NSCLC cell lines and their corresponding acquired VoR sublines. Cell viability was determined by the MTT assay (n = 3).	19440035 27086926
ELISA	IGF2; PubMed: 27086926 Oncogene Determination of vorinostat-induced IGF2 production by ELISA (n = 3). The conditioned mediums (CMs) obtained from cells treated with vorinostat (5 μM) for 2 days were used for ELISA. IL-13 / IL-10 / IL-5; PubMed: 18541724 Blood Effect of vorinostat on TH1/TH2 cytokine secretion in HL cell lines. Cells were incubated with medium or vorinostat (5 μM) for 24 hours and supernatants were examined for cytokine levels by ELISA. Each value represents a mean of 3 independent experiments done in triplicate (± SEM). M-CSF; PubMed: 23042302 Carcinogenesis Primary PyMT tumor cells were treated with SAHA (0-1000nM) for varying time points (8–48h), and supernatants were assayed by ELISA for M-CSF secretion. P < 0.05 and P < 0.001 versus control treatment. MMP-9; PubMed: 23042302 Carcinogenesis Primary PyMT tumor cells were treated with SAHA (0-1000nM) for varying time points (8–48h), and supernatants were assayed by ELISA for MMP-9 secretion. P < 0.05 and **P < 0.001 versus control treatment.	27086926 18541724 23042302

In vivo

Administration of Vorinostat (~100 mg/kg/day) significantly inhibits the growth of CWR22 human prostate xenografts in nude mice with tumor reductions of 78%, 97% and 97%, at doses of 25 mg/kg/day, 50 mg/kg/day and 100 mg/kg/day, respectively, compared with control. Vorinostat induces the accumulation of acetylated core histones and prostate-specific antigen mRNA expression in CWR22 cells, resulting in higher levels of serum prostate-specific antigen than predicted from tumor volume alone. ^[2] Oral administration of Vorinostat (0.67g/L) crosses the blood-brain barrier, increases histone acetylation in the brain, and dramatically improves the motor impairment in the R6/2 mice model of Huntington's disease. ^[5]

Protocol

Kinase Assay: ^[1]	- Collapse Immunoprecipitation-HDAC assays: The lysate of Jurkat cells is incubated for 1 hour on ice and cleared by centrifugation at 12,000 g for 10 minutes at 4 °C. Supernatants are precleared with 30 μL of 50% protein G-Sepharose slurry for 1 hour at 4 °C. Beads are pelleted by centrifugation and supernatants are incubated for 1 hour at 4 °C with 10 μg of IgG fraction from anti-HDAC1 or HDAC3 polyclonal antisera (preincubated 2 hours at room temperature with either the homologous or heterologous immunizing peptide). Both antisera are raised in rabbits against the carboxylterminal peptide of HDAC1 and HDAC3 by using synthetic peptides coupled to keyhole limpet hemocyanin. 30 μL of 50% protein G-Sepharose slurry is added for 1 hour at 4 °C. Immune complexes are pelleted by centrifugation and washed three times with 1 mL of lysis buffer. Beads are resuspended in 200 μL of HDAC buffer (20 mM Tris-HCl, pH 8.0/150 mM NaCl/10% glycerol), and the HDAC assay is performed with an ³H-acetylated peptide corresponding to amino acids 1-24 of histone H4. Released [³H]acetic acid is quantified by scintillation counting. For inhibitions studies, the immunoprecipitated complexes are preincubated with the different concentrations of Vorinostat for 30 minutes at 4 °C.
Cell Research: ^[2]	- Collapse Cell lines: LNCaP, PC-3, and TSU-Pr1 Concentrations: Dissolved in DMSO, final concentrations ~7.5 μM Incubation Time: 1, 2, 3 and 4 days Method: Cells are exposed to various concentrations of Vorinostat for 1, 2, 3 and 4 days. Cell viability is assessed by trypan blue dye exclusion. (Only for Reference)
Animal Research: ^[2]	- Collapse Animal Models: Male BALB/c nude (nu/nu) mice implanted with CWR22 tumor cells Dosages: ~100 mg/kg/day Administration: Injection i.p. (Only for Reference)

References

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Solubility (25°C)

In vitro	DMSO	52 mg/mL (196.74 mM)
	Water	Insoluble
	Ethanol	'3 mg/mL
In vivo	Add solvents to the product individually and in order(Data is from Selleck tests instead of citations): 2% DMSO+30% PEG 300+5% Tween 80+ddH2O For best results, use promptly after mixing.	5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information Download Vorinostat (SAHA) SDF

Molecular Weight	264.3
Formula	C₁₄H₂₀N₂O₃
CAS No.	149647-78-9
Storage	3 years-20°C powder 2 years-80°C in solvent
Synonyms	MK0683, Suberoylanilide hydroxamic acid
Smiles	C1=CC=C(C=C1)NC(=O)CCCCCCC(=O)NO

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Working concentration： mg/ml；

Method for preparing DMSO master liquid: ： mg drug pre-dissolved in μL DMSO (Master liquid concentration mg/mL， Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation：Take μL DMSO master liquid, next addμL PEG300， mix and clarify, next addμL Tween 80，mix and clarify, next add μL ddH₂O，mix and clarify.

Note：1.Please make sure the liquid is clear before adding the next solvent.
2.Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such as vortex, ultrasound or hot water bath can be used to aid dissolving.

Bio Calculators

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration _(start) x Volume _(start) = Concentration _(final) x Volume _(final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

Serial Dilutions
Concertration (start):
Dilution-folds:

Computed Result

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2021-01-06)

NCT Number	Recruitment	interventions	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT03022565	Withdrawn	Drug: Vorinostat	Uveal Melanoma	University of Miami\|University of Miami Sylvester Comprehensive Cancer Center	January 2020	Early Phase 1
NCT03167437	Recruiting	Drug: Vorinostat\|Drug: Ustekinumab	Crohn''s Disease	National Institute of Allergy and Infectious Diseases (NIAID)\|National Institutes of Health Clinical Center (CC)	October 30 2017	Phase 1\|Phase 2
NCT03056495	Recruiting	Drug: N-hydroxy-N''-phenyl-octanediamide (Vorinostat)	Alzheimer Disease	German Center for Neurodegenerative Diseases (DZNE)\|University Hospital Bonn\|University of Göttingen	September 28 2017	Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
What’s the recommendation about reconstitution of the compound for in vivo animal study?
Answer:
We recommend the following vehicle for S1047: 2% DMSO, 40% PEG 300, 5% Propylene glycol, 1% Tween 80 at 5 mg/mL.

HDAC Signaling Pathway Map

HDAC Inhibitors with Unique Features

Selective HDAC Inhibitor

RGFP966 : HDAC3-selective, IC50=80 nM.
Selective HDAC Inhibitor

Rocilinostat (ACY-1215) : HDAC5-selective, IC50=5 nM.
Most Potent HDAC Inhibitor

Quisinostat (JNJ-26481585) : HDAC1, IC50=0.11 nM; HDAC2, IC50=0.33 nM.
FDA-approved HDAC Inhibitor

Vorinostat (SAHA, MK0683) : Approved by FDA against cutaneous T cell lymphoma.
Newest HDAC Inhibitor

RG2833 (RGFP109) ^New : Brain-penetrant HDAC inhibitor with IC50 of 60 nM and 50 nM for HDAC1 and HDAC3, respectively.

Related HDAC Products

LMK-235 ^New

LMK-235 is a selective inhibitor of HDAC4 and HDAC5 with IC50 of 11.9 nM and 4.2 nM, respectively.
CAY10603 ^New

CAY10603 (BML-281) is a potent and selective HDAC6 inhibitor with IC50 of 2 pM, >200-fold selectivity over other HDACs.
Domatinostat (4SC-202) ^New

Domatinostat (4SC-202) is a selective class I HDAC inhibitor with IC50 of 1.20 μM, 1.12 μM, and 0.57 μM for HDAC1, HDAC2, and HDAC3, respectively. Also displays inhibitory activity against Lysine specific demethylase 1 (LSD1). Phase 1.
Panobinostat (LBH589)

Panobinostat (LBH589, NVP-LBH589) is a novel broad-spectrum HDAC inhibitor with IC50 of 5 nM in a cell-free assay. Panobinostat (LBH589) induces autophagy and apoptosis. Panobinostat effectively disrupts HIV latency in vivo. Phase 3.
Entinostat (MS-275)

Entinostat (MS-275, SNDX-275) strongly inhibits HDAC1 and HDAC3 with IC50 of 0.51 μM and 1.7 μM in cell-free assays, compared with HDACs 4, 6, 8, and 10. Entinostat induces autophagy and apoptosis. Phase 3.
Trichostatin A (TSA)

Trichostatin A (TSA) is an HDAC inhibitor with IC50 of ~1.8 nM in cell-free assays.
Romidepsin (FK228, Depsipeptide)

Romidepsin (FK228, Depsipeptide, FR 901228, NSC 630176) is a potent HDAC1 and HDAC2 inhibitor with IC50 of 36 nM and 47 nM in cell-free assays, respectively. Romidepsin (FK228/depsipeptide) controls growth and induces apoptosis in neuroblastoma tumor cells.

Features:More effective than other classical HDAC inhibitors such as TSA, TPX, and butyrate.
RGFP966

RGFP966 is an HDAC3 inhibitor with IC50 of 0.08 μM in cell-free assay, exhibits > 200-fold selectivity over other HDAC.
Tubastatin A

Tubastatin A is a potent and selective HDAC6 inhibitor with IC50 of 15 nM in a cell-free assay. It is selective against all the other isozymes (1000-fold) except HDAC8 (57-fold). Tubastatin A promotes autophagy and increases apoptosis.
Mocetinostat (MGCD0103)

Mocetinostat (MGCD0103, MG0103) is a potent HDAC inhibitor with most potency for HDAC1 with IC50 of 0.15 μM in a cell-free assay, 2- to 10- fold selectivity against HDAC2, 3, and 11, and no activity to HDAC4, 5, 6, 7, and 8. Mocetinostat (MGCD0103) induces apoptosis and autophagy. Phase 2.

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Vorinostat (SAHA)