Vorinostat (SAHA)

For research use only.

Catalog No.S1047 Synonyms: MK0683, Suberoylanilide hydroxamic acid

401 publications

Vorinostat (SAHA) Chemical Structure

CAS No. 149647-78-9

Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.

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Selleck's Vorinostat (SAHA) has been cited by 401 publications

Purity & Quality Control

Choose Selective HDAC Inhibitors

Biological Activity

Description Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.
Targets
HDAC [1]
(Cell-free assay)
~10 nM
In vitro

Vorinostat inhibits the activities of HDAC1 and HDAC3 with IC50 of 10 nM and 20 nM, respectively. Vorinostat also results in a marked hyperacetylation of histone H4. [1] Vorinostat inhibits the growth of three prostate cancer cell lines LNCaP, PC-3 and TSU-Pr1 at micromolar concentrations (2.5-7.5 μM), and induces dose-dependent cell death in LNCaP cells. [2] Vorinostat treatment in MCF-7 cells inhibits cell proliferation at an IC50 of 0.75 μM resulting in the accumulation of cells in the G1 and G2-M phase of the cell cycle. Vorinostat also induces differentiation in the estrogen receptor-negative cell line SKBr-3 and the retinoblastoma-negative cell line MDA-468. [3] Vorinostat treatment at 1 μM for 8 hours or more is sufficient to irreversibly induce apoptosis of human multiple myeloma (MM) cells. The gene expression profiles of Vorinostat treated MM cells are not hallmarked by global transcriptional activation, but by coordinated transcriptional changes of specific functional groups of genes such as cytokine-induced proliferative/survival signaling cascades, oncogenes-tumor suppressor genes, regulators of apoptosis, DNA synthesis-repair and cell cycle, and proteasome-ubiquitin function. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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Primary bronchial epithelial cell NVT5VGQ3U2mwYYPlJGF{e2G7 NIXNfpgyKM7:TR?= NYXHNm4xQTZiaB?= M4O4d2ROW09? NFXIZXpKdmirYnn0bY9vKG:oIFjERWM4KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJJNpd3K2IHPpdoN2cXRiY4XydoVvfHN? NVTnbnY3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUm5OlY4QDlpPkG5PVY3Pzh7PD;hQi=>
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CAL27 NYTn[mJLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXjte|N{PzJiaB?= MnvGTWM2OD1|LkeyJO69VQ>? NIfnUlc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N|c4QCd-MkCxOFM4Pzh:L3G+
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LNCAP NX71PW16T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmTaO|IhcA>? MlTLTWM2OD1yLkW4JO69VQ>? NXT6S2VvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM4PzhpPkKwNVQ{Pzd6PD;hQi=>
NCI-H2122 M{\zOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHCW5lHPzJiaB?= M4jYOGlEPTB;Nz61JO69VQ>? NIGzXFU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N|c4QCd-MkCxOFM4Pzh:L3G+
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SCC15 NXfkfopET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIfCeXA4OiCq M1O4c2lEPTB;Mj60PUDPxE1? Mnv5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO3O|goRjJyMUSzO|c5RC:jPh?=
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SKHEP1 M4[5fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3GzNlczKGh? NGfKbXpKSzVyPUOuOFYh|ryP M2TiPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzO|c5Lz5{MEG0N|c4QDxxYU6=
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Caco-2 NWfCcmVxU2mwYYPlJGF{e2G7 M4HIT|AvOzF4IH7NJJRwKDNzLk[g{txO Mmr5OEBp NX;OWZp2TE2VTx?= NUL5PZBQTXhidnn2c{BqdmirYnn0bY9vKG:oIHj1cYFvKEiGQVOxJJdqfGhiSVO1NEBw\iByLkGxPUDPxE1? M1TFTVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzPFQxLz5{MEG0N|g1ODxxYU6=
Caco-2 NITEXJpMcW6jc3WgRZN{[Xl? M33PXlAvOzF4IH7NJJRwKDNzLk[g{txO NVP1WoVRPCCq NHvPO3dFVVOR NV3LU2FtTXhidnn2c{BqdmirYnn0bY9vKG:oIHj1cYFvKEiGQVOzJJdqfGhiSVO1NEBw\iByLkG0O{DPxE1? NXPtOI1XRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
Caco-2 NXnNOI9zU2mwYYPlJGF{e2G7 MVK2MlMzKG6PIITvJFY{OiEQvF2= Mn7xOEBp MlziSG1UVw>? NF;LcY1GgCC4aY\vJIlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{Wge4l1cCCLQ{WwJI9nKDF{Lk[g{txO MmS1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
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Caco-2 MYnLbY5ie2ViQYPzZZk> MY[wMlMyPiCwTTD0c{A{OS54IN88US=> NV\0UpJYPCCq M{HK[WROW09? Mo\jSZghfmm4bzDpcohq[mm2aX;uJI9nKGi3bXHuJGhFSUN4 MVG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
HepG2 NGTJTnpMcW6jc3WgRZN{[Xl? MWK1JO69VQ>? MmP0Oog> NFPzUlhFVVOR M4PyOWlv\HWldHnvckBw\iCKRFHDMY1m\GmjdHXkJIhqe3SxbnWgZYNmfHmuYYTpc44> NH[4WXo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N|g1OCd-MkCxOFM5PDB:L3G+
HepG2 M2O3TGtqdmG|ZTDBd5NigQ>? NITSUWQ2KM7:TR?= NVLlbpg4Pmh? M3n5TWROW09? NVLxc5pUUW6mdXP0bY9vKG:oIFjERWMudWWmaXH0[YQhcGm|dH;u[UBpgXCncnHj[ZR6dGG2aX;u NFLzbmw9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N|g1OCd-MkCxOFM5PDB:L3G+
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HepG2 NHy0WXpMcW6jc3WgRZN{[Xl? MmHxOUDPxE1? MYC2bC=> MVvEUXNQ NH31eWhKdmS3Y4Tpc44hd2ZiSFTBR{1u\WSrYYTl[EBJOy:KNDDobZN1d26nIHHj[ZR6dGG2aX;u M3f5dVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyMUSzPFQxLz5{MEG0N|g1ODxxYU6=
COLO205 MYfLbY5ie2ViQYPzZZk> M{\nflExKM7:TR?= M2\1OlI1KGh? NUS0TppoTE2VTx?= M{\1[WlvcGmkaYTpc44hd2ZiSFTBR|Eh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIhqe3SxbnWgTFMh[WOndInsZZRqd25id3n0bEBKSzVyIH;mJFIvOiEQvF2= M2XBVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEWxN|c5Lz5{MES1NVM4QDxxYU6=
SCC4 M3i0bWtqdmG|ZTDBd5NigQ>? M2frUlEh|ryP NWTJOHNVOjSq NEOwVYRKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6geJVjfWyrbjDhZ4V1gWyjdHnvci=> MmjQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2NUKyNlUoRjJyNEWyNlI2RC:jPh?=
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NB4 NITFWYVMcW6jc3WgRZN{[Xl? MmDNOUDPxE1? NH;BXpIzPCCq NYn3NWJ5TE2VTx?= MlryTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViSEOgZYNmfHmuYYTpc44> M{\OR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEmxOFQxLz5{MES5NVQ1ODxxYU6=
NUGC3 M{XxPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVfHTVUxRTJwOUSg{txO NYDPRoVCRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4OVA6PzFpPkKwPFUxQTdzPD;hQi=>
A549 NXPx[HptTnWwY4Tpc44hSXO|YYm= MojQOUDPxE1? MnrFN{Bp M3zrfGROW09? MkjuTY5lfWO2aX;uJI9nKGG3dH;wbIFogSCrbjDoeY1idiCDNUS5JINmdGy|IHHzd4V{e2WmIHHzJIlv[3KnYYPlJIlvKEyFM1nJ MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh3NUKwPEc,OjB6NUWyNFg9N2F-
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U937 NYXjUJRFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NITRV5U4OiCq NXPZOHdpTE2VTx?= NVP5bo1IT0l3ME2wMlM6KM7:TR?= MXq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODh6NEKwPEc,OjB6OESyNFg9N2F-
A431 NUDyW25RS3m2b4TvfIlkKEG|c3H5 M{TqUVczKGh? M1zCVWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE1OzFiY3XscJMhd3[ncnX4dJJme3OrbnegSWdHWiC5aYToJGlEPTBib3[gOE4zKM7:TR?= MofoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2NlkoRjJzMEiwOlI6RC:jPh?=
A549 MoDkR5l1d3SxeHnjJGF{e2G7 NXvNXolJPzJiaB?= NIe5W5ZEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBCPTR7IHPlcIx{KG:4ZYLlfJBz\XO|aX7nJGhFSUNid3n0bEBKSzVyIH;mJFEvQCEQvF2= MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
CAL27 MnrZR5l1d3SxeHnjJGF{e2G7 M1;NRlczKGh? NUHXbYM1S3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hS0GOMkegZ4VtdHNib4\ldoV5eHKnc4PpcochTUeIUjD3bZRpKEmFNUCgc4YhOy5{IN88US=> NH7jcpI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFYzQSd-MkGwPFA3Ojl:L3G+
HeLa MXTDfZRwfG:6aXOgRZN{[Xl? Mo\YO|IhcA>? M1vkOGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGhmVGFiY3XscJMhd3[ncnX4dJJme3OrbnegTGRCSyC5aYToJGlEPTBib3[gNU42KM7:TR?= MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
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COR-L23 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXWxNEDPxE1? MnXxO|IhcA>? MkD0SG1UVw>? NF\jV|VIUTVyPUGuNUDPxE1? NFOwOVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFY1Pyd-MkGwPFA3PDd:L3G+
DU145 NFXRc|ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYrweJQ2OTBizszN M{n2[lczKGh? MYfEUXNQ NH3qV49IUTVyPUGuNFUh|ryP M3;TSVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEiwOlQ4Lz5{MUC4NFY1PzxxYU6=
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MCR5-SV2 Mor4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXBflEyOCEQvF2= NXf1VYRIPzJiaB?= MXnEUXNQ M{HzZ2dKPTB;Mj6xJO69VQ>? NXrXNnBRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3PDdpPkKxNFgxPjR5PD;hQi=>
HCT116 NInvRWlMcW6jc3WgRZN{[Xl? NHf3VJc{OCEQvF2= NHTlNZg{OCCq NWLFUVhwTE2VTx?= NV60e2Q3UW6qaXLpeIlwdiCxZjDISGFEPiCjc4Pld5Nm\CCjczDpcoR2[3Srb36gc4Yh[WyyaHGteJVjfWyrbjDhZ4V1gWyjdHnvci=> M2PDR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMEiwOlQ4Lz5{MUC4NFY1PzxxYU6=
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U937 MkizT4lv[XOnIFHzd4F6 NGP4T5Y2KM7:TR?= M{DIVVI1KGh? MnvUSG1UVw>? M322[2lvcGmkaYTpc44hd2ZiaHnzeI9v\SCjY3X0fYx1emGwc3\ldoF{\SCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDv[kBi[2W2eXzheIlwdiCxZjDobZN1d26nIFizJIF1KEt7 MWe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTJ7MkS5Nkc,OjF{OUK0PVI9N2F-
U937 NHrNS2FMcW6jc3WgRZN{[Xl? NXLye4hiPSEQvF2= MXqyOEBp M{\1cGROW09? M1Hm[2lvcGmkaYTpc44hd2ZiaHnzeI9v\SCjY3X0fYx1emGwc3\ldoF{\SCjc4Pld5Nm\CCjczDpcoNz\WG|ZTDv[kBi[2W2eXzheIlwdiCxZjDobZN1d26nIFizJIF1KEtzOB?= Ml7XQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF{OUK0PVIoRjJzMkmyOFkzRC:jPh?=
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A2780 NF;DdlZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVnVPYhuQTZiaB?= MnrCSG1UVw>? NV;1eW5kUUN3ME2xMlYzKM7:TR?= NVL1UIxvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG2N|Q1OzBpPkKxOlM1PDNyPD;hQi=>
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Hep3B M4Hwcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TiU|Q5KGh? MoDaSG1UVw>? MkXsS2k2OD1zLkKxJO69VQ>? MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTdzMkG0Okc,OjF5MUKxOFY9N2F-
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DU145 NIDubndMcW6jc3WgRZN{[Xl? MorBNk42KM7:TR?= NHzrZWQzPCCq M{DVU2ROW09? NXribFBIUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IIXwdoVofWyjdHnvckBw\iCyMkH3ZYYyKHC{b4TlbY4h\XiycnXzd4lwdg>? Ml;pQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{NkCxOlYoRjJ{Mk[wNVY3RC:jPh?=
DU145 NX7SNHRCU2mwYYPlJGF{e2G7 NWnsbmlCPSEQvF2= NFG2bZMzPCCq MVHEUXNQ NHX4RlBKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaHnzeI9v\SCKNDDhZ4V1gWyjdHnvci=> MljKQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ{NkCxOlYoRjJ{Mk[wNVY3RC:jPh?=
DU145 MljKT4lv[XOnIFHzd4F6 NYP5NmRNPSEQvF2= NILMPIQzPCCq Mk\YSG1UVw>? NYTKboVvUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHjpd5RwdmViSEOgbJlx\XKjY3X0fYxifGmxbh?= NYSzWlZVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKyOlAyPjZpPkKyNlYxOTZ4PD;hQi=>
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A549/ATCC Mm\sS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnv4Ok4zKM7:TR?= NVjnZ4pyPzJiaB?= M4XR[GROW09? MUnHTVUxRTFwNjFOwG0> Ml3iQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
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NCI-H23 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXi2MlIh|ryP NVLmT4w{PzJiaB?= M4HtVGROW09? MoWyS2k2OD1zIN88US=> M13DSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
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OVCAR5 MmfES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3KOIk3NjJizszN NF23d4E4OiCq NWP6TJhTTE2VTx?= NHfJd5BIUTVyPUCuO|kh|ryP MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
OVCAR8 MnraS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHfSPZY3NjJizszN Ml\lO|IhcA>? NWTwbHROTE2VTx?= NY\EUFRPT0l3ME2wMlUh|ryP NXzEfpFRRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
RXF393 MmTHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWT0[VY2Pi5{IN88US=> MnLoO|IhcA>? MYjEUXNQ NGq5XVNIUTVyPUGuN{DPxE1? MlHRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
SF268 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXr5S|JkPi5{IN88US=> MVu3NkBp NV;YT5JQTE2VTx?= M{jL[2dKPTB;MT62JO69VQ>? MkLuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
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Hep3B MnHXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnWUpo1QCCq M1\IZmROW09? MWnHTVUxRTBwNkmg{txO NFrrbHE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzPVg3Oyd-MkK0N|k5PjN:L3G+
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BGC823 NYnoVm9VT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mm\SO|IhcA>? MoXVSG1UVw>? M1W1O2dKQTB;Nz61JO69VQ>? MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MUO3Okc,OjNyNkGzO|Y9N2F-
MGC803 NUHlSo5XT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYrYR|BUPzJiaB?= MnXOSG1UVw>? MYDHTVkxRTFyLkig{txO MV:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MUO3Okc,OjNyNkGzO|Y9N2F-
SGC7901 NF:x[VZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1rROlczKGh? MWnEUXNQ MV3HTVkxRTlwNDFOwG0> NGHjXpU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
SKHEP1 NGqyN|VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlvlO|IhcA>? NUn2NFI6TE2VTx?= MnLMS2k6OD1zNzFOwG0> NETROm09[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
SMMC7721 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEH1cnQ4OiCq NULLZYFJTE2VTx?= NUTKUnF1T0l7ME2yNEDPxE1? NEG5PYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
NUGC3 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWSxNEDPxE1? NEH4ZVBIUT1{Lkm0JO69VQ>? NW\s[XRiRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOxOlM{OzJpPkKzNVY{OzN{PD;hQi=>
A2780 CisR MmnYT4lv[XOnIFHzd4F6 NInUWmYyQCCq NUX6TJI{UW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlQ1KM7:TR?= M{Drb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
CAL27 sens MUnLbY5ie2ViQYPzZZk> MoXONVghcA>? M175bmlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD6zOkDPxE1? MkLqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN{NUK2NFMoRjJ|MkWyOlA{RC:jPh?=
CAL27 CisR MUfLbY5ie2ViQYPzZZk> NXL1SoVlOThiaB?= Mne2TY5pcWKrdHnvckBw\iCKRFHDJJdqfGhiSVO1NEBw\iByLk[xJO69VQ>? M1vpPFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
KYSE-510 CisR MWLLbY5ie2ViQYPzZZk> NEW0c|MyQCCq NWDufHZlUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlU6KM7:TR?= NUHDbo1nRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
KYSE-510 sens MnLGT4lv[XOnIFHzd4F6 M2nRc|E5KGh? MXfJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNzFOwG0> M1y4W|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
MDA-MB-231 sens NXv1NpBmU2mwYYPlJGF{e2G7 NXroN5JrOThiaB?= M3KwWmlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD62NUDPxE1? NUi0cnFFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
MDA-MB-231 CisR MXfLbY5ie2ViQYPzZZk> M1XPXFE5KGh? MWjJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNkGg{txO M4TNd|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|MkWyOlA{Lz5{M{K1NlYxOzxxYU6=
ES2 NV6xXGpnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXvvW44yPDhiaB?= MmnDSG1UVw>? Mk[2TWM2OD1zMj63JO69VQ>? NULYW2o1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVM1PDlpPkKzOFk{PDR7PD;hQi=>
MDA-MB-231 MoS4T4lv[XOnIFHzd4F6 NFvQeXEyOCEQvF2= MWWzNEBucW5? MoTsSG1UVw>? NYLCRlJlUW6qaXLpeIlwdiCxZjDISGFEOmF? NX7lVY91RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVM1PDlpPkKzOFk{PDR7PD;hQi=>
MDA-MB-231 M13kTmtqdmG|ZTDBd5NigQ>? NHnBRpIyKM7:TR?= M4jEUFI1KGh? NHi5Z3ZFVVOR MnPGTY5pcWKrdHnvckBw\iCKRFHDOkBie3Onc4Pl[EBieyCjY3X0fYxifGmxbjDs[ZZmdHNib3[geJVjfWyrbh?= NXO4TVQyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVM1PDlpPkKzOFk{PDR7PD;hQi=>
HS68 M3n0O2N6fG:2b4jpZ{BCe3OjeR?= NEX1[4UyOCEQvF2= M2DTW|czKGh? MWrEUXNQ Ml[1TWM2OD12Lk[g{txO M1G5U|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUewOVQzLz5{M{W3NFU1OjxxYU6=
MEF MVXDfZRwfG:6aXOgRZN{[Xl? NXjHcIlYOTBizszN MV[3NkBp Mni3SG1UVw>? NH;OZY1KSzVyPUSuOEDPxE1? M3zsbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUewOVQzLz5{M{W3NFU1OjxxYU6=
BGC823 NICzW|JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4LMOGROW09? MWDHTVUxRTNwMEmg{txO Mkn2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4MEG3NFYoRjJ|NkCxO|A3RC:jPh?=
High5 M4nqS2tqdmG|ZTDBd5NigQ>? MnHUNlQhcA>? MWfJcohq[mm2aX;uJI9nKGi3bXHuJJJm[2:vYnnuZY51KEiGQVO2JJdqfGhiSVO1NEBw\iByLkC5JO69VQ>? M1\jPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkCyOVI{Lz5{M{[wNlUzOzxxYU6=
High5 M1uxXmtqdmG|ZTDBd5NigQ>? MXSyOEBp NYXqPVFPUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEOSC5aYToJGlEPTBib3[gNE4yOiEQvF2= MkPwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4MEK1NlMoRjJ|NkCyOVI{RC:jPh?=
High5 M3fhRWtqdmG|ZTDBd5NigQ>? NYfLUJE1OjRiaB?= MkfVTY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDN{B4cXSqIFnDOVAhd2ZiMD6xO{DPxE1? MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzZyMkWyN{c,OjN4MEK1NlM9N2F-
A549 NV3BT|lFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;EcWVHTE2VTx?= MlvoTWM2OD1zLkC3PUDPxE1? M2r4TVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkS0NlExLz5{M{[0OFIyODxxYU6=
HCT116 NIKyOIVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVfEUXNQ NE\ZdFZKSzVyPUCuO|U4KM7:TR?= NXrtN2dVRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2OFQzOTBpPkKzOlQ1OjFyPD;hQi=>
K562 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVTEUXNQ MmjUTWM2OD1yLk[0OUDPxE1? MmPRQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NESyNVAoRjJ|NkS0NlExRC:jPh?=
GBC-SD MlzES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW[1JO69VQ>? Ml;aO|IhcA>? NY\QN3dHTE2VTx?= MkC5TWM2OD1zND64JO69VQ>? NYrFXIx4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFczPjBpPkKzO|A4OjZyPD;hQi=>
HGC27 NIjRd2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnKxOUDPxE1? M3L1PFczKGh? M{TQcmROW09? MWrJR|UxRTVwNDFOwG0> NYrGRohxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO3NFczPjBpPkKzO|A4OjZyPD;hQi=>
HL60 MmW2T4lv[XOnIFHzd4F6 MmXSOUDPxE1? MXKyOEBp Mme4SG1UVw>? NGL4NYJKdmirYnn0bY9vKG:oIFjERWM> M3jLc|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C3NlYxLz5{M{ewO|I3ODxxYU6=
Vero MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M17xblczKGh? NHHQclBFVVOR M{O0V2lEPTB;MD65JO69VQ>? NH33bVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{e4OlQ2Oid-MkO3PFY1PTJ:L3G+
NCI60 NFrjfopEgXSxdH;4bYMhSXO|YYm= NGfnfVM1QCCq M2j2TWROW09? NFrEdm5IUT1yLkWzJO69VQ>? NY\2TnBFRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSwNVU{OjdpPkK0NFE2OzJ5PD;hQi=>
SW620 MUnDfZRwfG:6aXOgRZN{[Xl? MnXJOFghcA>? M172fmROW09? NHfsVXRIUT1yLkW0JO69VQ>? M3XWVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEG1N|I4Lz5{NECxOVMzPzxxYU6=
U251 NVrxOYhrS3m2b4TvfIlkKEG|c3H5 NUHDWJZmPDhiaB?= MV7EUXNQ Mn;HS2k:OS53MzFOwG0> NIHyb|Q9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NECxOVMzPyd-MkSwNVU{Ojd:L3G+
KM12 MUDDfZRwfG:6aXOgRZN{[Xl? NF7kRpY1QCCq MlvOSG1UVw>? NWm5T2JTT0l;MT64PEDPxE1? MoCyQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUWzNlcoRjJ2MEG1N|I4RC:jPh?=
Hs578T MnHCR5l1d3SxeHnjJGF{e2G7 NXni[4lWPDhiaB?= NIDKWGNFVVOR NX7xbHFXT0l;ND64N{DPxE1? MmjxQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUWzNlcoRjJ2MEG1N|I4RC:jPh?=
MDA-MB-231 M4TwTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1jhdlQ5KGh? MUTJR|UxRTBwMU[g{txO M4LOS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MEm1NFE3Lz5{NEC5OVAyPjxxYU6=
MCF7 MnHYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlX5OFghcA>? NXe4dVhMT0l;MD60OUDPxE1? M2DDeFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2MUG5OVU2Lz5{NEGxPVU2PTxxYU6=
MDA-MB-435 M{nkdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF3WVGg1QCCq MnnuS2k:OC56ODFOwG0> NUPy[mhHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSxNVk2PTVpPkK0NVE6PTV3PD;hQi=>
AsPC1 M1XyU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGPoZoE{OCEQvF2= NWHz[ZJkPDhiaB?= MUTEUXNQ NUHlSIZQUUN3ME2zMlY3KM7:TR?= NETKRpM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEG4OVM4QCd-MkSxPFU{Pzh:L3G+
Jurkat E6.1 MYrLbY5ie2ViQYPzZZk> MlK0NkBp NFOwN|ZFVVOR M2ryRWlvcGmkaYTpc44hd2ZiSFTBR|Qhf2m2aDDJR|UxKG:oIECuNFQh|ryP MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDJ4MUi2Nkc,OjR{NkG4OlI9N2F-
KG1 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnr4OFghcA>? NFXRUlFKSzVyPUGuNVUh|ryP MUG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV{NUCwN{c,OjR3MkWwNFM9N2F-
HEL MkfmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV:zWnNvPDhiaB?= NXj5RmFMTE2VTx?= NG\CWZBKSzVyPUCuOFkh|ryP NUHVZZVPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS2PVQxPTVpPkK0Olk1ODV3PD;hQi=>
HSC3 M36we2N6fG:2b4jpZ{BCe3OjeR?= NIP2PJUyOiCq M2XNfmROW09? MXTJR|UxRTBwN{Gg{txO M4jENlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{[2OVYxLz5{NEe2OlU3ODxxYU6=
HT-29 Ml3jR5l1d3SxeHnjJGF{e2G7 Mne3NVIhcA>? NFHT[nhFVVOR NYK1[HR[UUN3ME2wMlczKM7:TR?= NGjBPJo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2OlU3OCd-MkS3OlY2PjB:L3G+
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H460 Mlf2R5l1d3SxeHnjJGF{e2G7 M3rUblEzKGh? MmLRSG1UVw>? MmrzTWM2OD1yLkiyJO69VQ>? NXjOeYpZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS3OlY2PjBpPkK0O|Y3PTZyPD;hQi=>
MONE1 NWfQfIpjS3m2b4TvfIlkKEG|c3H5 NV7jWmNoOTJiaB?= NX\P[G5CTE2VTx?= MXXJR|UxRTFwMUig{txO MWG8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4NkW2NEc,OjR5Nk[1OlA9N2F-
MKN45 NWjwfYNIS3m2b4TvfIlkKEG|c3H5 NUXoUVB1OTJiaB?= MmWwSG1UVw>? MkTZTWM2OD1zLkK1JO69VQ>? M37rN|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{[2OVYxLz5{NEe2OlU3ODxxYU6=
TSGH NFf2d4VEgXSxdH;4bYMhSXO|YYm= NX61RnJEOTJiaB?= MkK0SG1UVw>? MYXJR|UxRTJwMEmg{txO NGn3UJA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2OlU3OCd-MkS3OlY2PjB:L3G+
HL60 NI[2boJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDNb|VIPzJiaB?= MUnEUXNQ NIj5cnlIUTVyPUCuPVMh|ryP NHrpSGo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFUxOCd-MkS5NFA2ODB:L3G+
HEK293 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NW\mfmpEPzJiaB?= NFj5WllFVVOR MUTHTVUxRTF|IN88US=> NVHIWIRDRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkS5NFA2ODBpPkK0PVAxPTByPD;hQi=>
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UACC-893 NYXmRWxyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEW3S2xKSzVyPUKzMlExOzhizszN M{f5S|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H2347 NFP3[pJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUPGUohxUUN3ME2yN{4zPjd2IN88US=> Ml7nQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
U-118-MG MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTJ|LkeyNVch|ryP M4nxW|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H596 NGTiRpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYrJR|UxRTJ|LkmwOVUh|ryP MlfuQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
MZ1-PC NXzKV2pmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jGWWlEPTB;MkSuOlc2QSEQvF2= NY[w[WFwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
SW1417 NYjBb284T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUO1SmQ4UUN3ME2yOU4zPDl6IN88US=> MkLEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NCI-H82 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFOxSm9KSzVyPUK2MlQzQDhizszN NXT6dVdxRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
COLO-678 NEjofJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmD2TWM2OD1{Nz60PVE6KM7:TR?= Mn30QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
KURAMOCHI Mki3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{HXRmlEPTB;MkiuNVI3KM7:TR?= M1jK[FxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H1650 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFf2PJJKSzVyPUOwMlE6OjRizszN M2XRUlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
HCC1954 NEX1RVNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVfORXMyUUN3ME2zNU4{PjZ4IN88US=> NWTMUlY5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+
GAK MnL1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Ml;YTWM2OD1|Mj6yOlE3KM7:TR?= MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
YAPC NH7TdG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPDTWM2OD1|Mz63OlQ5KM7:TR?= NFnmOW89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NCI-H2228 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVLFflF2UUN3ME2zOE4{PjB3IN88US=> NEnQWoU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
VMRC-RCZ NIjxNGtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWLJR|UxRTN4LkOwNlIh|ryP MmDTQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NMC-G1 M3Pw[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHj0c25KSzVyPUO3MlA2OjlizszN NIToNYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
D-336MG M2jS[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJR|UxRTRzLki0OFIh|ryP MYS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:5d4eu[YJqNmGlLoXrM4Np\W2kbD;jc41xd3WwZG;y[ZBwenShY3Hy[E9EUEWPQly5PE8oRlODTlfFVlww[T5?
UACC-62 MmPXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3HidmlEPTB;NEKuOVUxPSEQvF2= NHTSdG49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
C-33-A MoCzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPNcIl6UUN3ME20OU4{OjV4IN88US=> NGHOcWk9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
HCC1937 MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4HURmlEPTB;NEeuNVk2QCEQvF2= NH;TcXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
HDLM-2 NWHGWFkzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHrHeHpKSzVyPUS3MlIzQDdizszN M3rkR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4f3e{5m[mlwYXOueYsw[2inbXLsM4NwdXCxdX7kY5JmeG:{dG;jZZJlN0OKRV3CUFk5Nyd-U1HOS2VTRC:jPh?=
NCI-H727 MoXES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NG\sW4RKSzVyPUS3MlI1PTFizszN MkHPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xd4f3MoVjcS6jYz71b{9kcGWvYnyvZ49ueG:3bnTfdoVxd3K2X3PhdoQwS0iHTVLMPVgwLz6VQV7HSXI9N2F-
NY NFvtVXZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUPJZVFQUUN3ME20PU4{QTB6IN88US=> NGLW[Y49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;3e5cv\WKrLnHjMpVsN2OqZX3icE9kd22yb4Xu[H9z\XCxcoTfZ4Fz\C:FSFXNRmw6QC9pPmPBUmdGWjxxYU6=
NCI-H2291 NVy4d3pWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmroTWM2OD12OT64N|AyKM7:TR?= NYnrdlFWRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:ve5d4NmWkaT7hZ{52cy:laHXtZoww[2:vcH;1coRgemWyb4L0Y4NiemRxQ1jFUWJNQThxJ{7TRW5ITVJ:L3G+

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
Ac-Histone H4 / Ac-Histone H3; 

PubMed: 21598070     


D54 and U87 glioma cells were treated with lower concentrations (1-3 µM) of vorinostat. The effects on histone acetylation was assessed by immunoblotting. 

phospho-CDK1; 

PubMed: 21598070     


Changes in level of inhibitory phosphorylation of cdk1 after treatment with vorinostat was determined at 24 and 48 h (using a Tyr-15 residue phospho-specific antibody) along with total cdk1 as loading control. 

phospho-Cdc25c / Cdc25c; 

PubMed: 21598070     


Time course of changes in phospho-cdc25c levels after exposure to vorinostat (5 µM).

Cyclin B1; 

PubMed: 21598070     


Cyclin B1 levels were determined in D54 and U87 cells after treatment with various doses of vorinostat at 24 and 48 h.

c-Myc / c-Raf / Akt / Cyclin D1 / CDK4; 

PubMed: 19440035     


Cell lysates from JeKo-1 and Mo2058 cells exposed to vorinostat  were immunoblotted for c-Myc, c-Raf, AKT, cyclin D1, CDK4 and acetylated histone H3. The levels of β-actin served as the loading control.

p-eIF2α / ATF4 / CHOP ; 

PubMed: 20072656     


Western blot was performed to evaluate activated eIF2α (P-eIF2α), translational up-regulation of ATF4, and CHOP expression in U251 cells after vorinostat exposure.

p21; 

PubMed: 21598070     


To determine the dose response relationship to vorinostat, cells were exposed to various higher concentrations (5–10 µM) of the agent and the levels of p21 determined by immunoblotting.

21598070 19440035 20072656
Immunofluorescence
HDAC1 / CK19; 

PubMed: 29917299     


Immunocytochemistry to detect the expression of HDAC1 and CK 19 in PDAC cells with/without Vorinostat treatment. HDAC1 and CK19 were stained red and green, respectively. The Hoechst‐stained nuclei appear blue. Scale bar: 100 μm. The CK19 expression in PSN1 and MiaPaCa2 was increased by Vorinostat treatment.

Nrf2; 

PubMed: 20566897     


Vorinostat induced translocation of Nrf2 from cytosol to nucleus. U937 cells were treated with 2μM vorinostat for 18 hours and then cytospun. Cells were fixed and labeled for Nrf2 protein (red) and nucleus (blue, 4,6-diamidino-2-phenylindole) as described in “Immunofluorescence and confocal microscopy.” Images were taken by Nikon Eclipse TE2000 confocal microscope with 40×/1.30 numeric aperture oil objective lens. Ctrl indicates control cells without treatment; and Vor, vorinostat.

α-SMA; 

PubMed: 26330748     


Representative immunofluorescence images and the quantification graph showing the effect of vorinostat (2.5 µM), TGIF1, and TGIF2 siRNA knockdown on αSMA. Control human corneal fibroblast cultures (-TGFβ) showed no αSMA staining. Cultures treated with TGFβ1 (5 ng/ml) showed a robust αSMA staining. Vorinostat treatment markedly attenuated TGFβ-evoked αSMA staining. The knockdown of TGIF1 (+TGFβ+vorinostat+TGIF1siRNA) and TGIF2 alone (+TGFβ+vorinostat+TGIF2siRNA) or in combination (+TGFβ +vorinostat +TGIF1 +2siRNA) markedly blocked the effect of vorinostat on αSMA. Scale bar=100 μm.

Ac-STAT3 / IGF2; 

PubMed: 27086926     


Immunofluorescence staining of Ac-STAT3 and IGF2 after vorinostat treatment.

29917299 20566897 26330748 27086926
Growth inhibition assay
Cell apoptosis; 

PubMed: 19440035     


Cells were treated with indicated concentrations of vorinostat for 48 hours. Following this, the percentage of annexin-V-stained apoptotic cells was determined by flow cytometry. Values represented as bar graphs are the mean of 3 experiments plus or minus the standard error (SE)

Cell viability; 

PubMed: 27086926     


The effects of vorinostat on the viability of the indicated NSCLC cell lines and their corresponding acquired VoR sublines. Cell viability was determined by the MTT assay (n = 3).

19440035 27086926
ELISA
IGF2; 

PubMed: 27086926     


Determination of vorinostat-induced IGF2 production by ELISA (n = 3). The conditioned mediums (CMs) obtained from cells treated with vorinostat (5 μM) for 2 days were used for ELISA. 

IL-13 / IL-10 / IL-5; 

PubMed: 18541724     


Effect of vorinostat on TH1/TH2 cytokine secretion in HL cell lines. Cells were incubated with medium or vorinostat (5 μM) for 24 hours and supernatants were examined for cytokine levels by ELISA. Each value represents a mean of 3 independent experiments done in triplicate (± SEM).

M-CSF; 

PubMed: 23042302     


Primary PyMT tumor cells were treated with SAHA (0-1000nM) for varying time points (8–48h), and supernatants were assayed by ELISA for M-CSF  secretion. *P < 0.05 and **P < 0.001 versus control treatment. 

MMP-9; 

PubMed: 23042302     


Primary PyMT tumor cells were treated with SAHA (0-1000nM) for varying time points (8–48h), and supernatants were assayed by ELISA for MMP-9 secretion. *P < 0.05 and **P < 0.001 versus control treatment. 

27086926 18541724 23042302
In vivo Administration of Vorinostat (~100 mg/kg/day) significantly inhibits the growth of CWR22 human prostate xenografts in nude mice with tumor reductions of 78%, 97% and 97%, at doses of 25 mg/kg/day, 50 mg/kg/day and 100 mg/kg/day, respectively, compared with control. Vorinostat induces the accumulation of acetylated core histones and prostate-specific antigen mRNA expression in CWR22 cells, resulting in higher levels of serum prostate-specific antigen than predicted from tumor volume alone. [2] Oral administration of Vorinostat (0.67g/L) crosses the blood-brain barrier, increases histone acetylation in the brain, and dramatically improves the motor impairment in the R6/2 mice model of Huntington's disease. [5]

Protocol

Kinase Assay:

[1]

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Immunoprecipitation-HDAC assays:

The lysate of Jurkat cells is incubated for 1 hour on ice and cleared by centrifugation at 12,000 g for 10 minutes at 4 °C. Supernatants are precleared with 30 μL of 50% protein G-Sepharose slurry for 1 hour at 4 °C. Beads are pelleted by centrifugation and supernatants are incubated for 1 hour at 4 °C with 10 μg of IgG fraction from anti-HDAC1 or HDAC3 polyclonal antisera (preincubated 2 hours at room temperature with either the homologous or heterologous immunizing peptide). Both antisera are raised in rabbits against the carboxylterminal peptide of HDAC1 and HDAC3 by using synthetic peptides coupled to keyhole limpet hemocyanin. 30 μL of 50% protein G-Sepharose slurry is added for 1 hour at 4 °C. Immune complexes are pelleted by centrifugation and washed three times with 1 mL of lysis buffer. Beads are resuspended in 200 μL of HDAC buffer (20 mM Tris-HCl, pH 8.0/150 mM NaCl/10% glycerol), and the HDAC assay is performed with an 3H-acetylated peptide corresponding to amino acids 1-24 of histone H4. Released [3H]acetic acid is quantified by scintillation counting. For inhibitions studies, the immunoprecipitated complexes are preincubated with the different concentrations of Vorinostat for 30 minutes at 4 °C.
Cell Research:

[2]

- Collapse
  • Cell lines: LNCaP, PC-3, and TSU-Pr1
  • Concentrations: Dissolved in DMSO, final concentrations ~7.5 μM
  • Incubation Time: 1, 2, 3 and 4 days
  • Method:

    Cells are exposed to various concentrations of Vorinostat for 1, 2, 3 and 4 days. Cell viability is assessed by trypan blue dye exclusion.


    (Only for Reference)
Animal Research:

[2]

- Collapse
  • Animal Models: Male BALB/c nude (nu/nu) mice implanted with CWR22 tumor cells
  • Dosages: ~100 mg/kg/day
  • Administration: Injection i.p.
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 52 mg/mL (196.74 mM)
Water Insoluble
Ethanol '3 mg/mL
In vivo Add solvents to the product individually and in order(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.
5mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 264.3
Formula

C14H20N2O3

CAS No. 149647-78-9
Storage powder
in solvent
Synonyms MK0683, Suberoylanilide hydroxamic acid
Smiles C1=CC=C(C=C1)NC(=O)CCCCCCC(=O)NO

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Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

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To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03022565 Withdrawn Drug: Vorinostat Uveal Melanoma University of Miami|University of Miami Sylvester Comprehensive Cancer Center January 2020 Early Phase 1
NCT03167437 Recruiting Drug: Vorinostat|Drug: Ustekinumab Crohn''s Disease National Institute of Allergy and Infectious Diseases (NIAID)|National Institutes of Health Clinical Center (CC) October 30 2017 Phase 1|Phase 2
NCT03056495 Recruiting Drug: N-hydroxy-N''-phenyl-octanediamide (Vorinostat) Alzheimer Disease German Center for Neurodegenerative Diseases (DZNE)|University Hospital Bonn|University of Göttingen September 28 2017 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

  • Question 1:

    What’s the recommendation about reconstitution of the compound for in vivo animal study?

  • Answer:

    We recommend the following vehicle for S1047: 2% DMSO, 40% PEG 300, 5% Propylene glycol, 1% Tween 80 at 5 mg/mL.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID