Vorinostat (SAHA)

Catalog No.S1047 Synonyms: MK0683, Suberoylanilide hydroxamic acid, Zolinza

For research use only.

Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683, Zolinza) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.

Vorinostat (SAHA) Chemical Structure

CAS No. 149647-78-9

Selleck's Vorinostat (SAHA) has been cited by 473 publications

Purity & Quality Control

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Biological Activity

Description Vorinostat (suberoylanilide hydroxamic acid, SAHA, MK0683, Zolinza) is an HDAC inhibitor with IC50 of ~10 nM in a cell-free assay. Vorinostat abrogates productive HPV-18 DNA amplification.
Targets
HDAC [1]
(Cell-free assay)
~10 nM
In vitro

Vorinostat inhibits the activities of HDAC1 and HDAC3 with IC50 of 10 nM and 20 nM, respectively. Vorinostat also results in a marked hyperacetylation of histone H4. [1] Vorinostat inhibits the growth of three prostate cancer cell lines LNCaP, PC-3 and TSU-Pr1 at micromolar concentrations (2.5-7.5 μM), and induces dose-dependent cell death in LNCaP cells. [2] Vorinostat treatment in MCF-7 cells inhibits cell proliferation at an IC50 of 0.75 μM resulting in the accumulation of cells in the G1 and G2-M phase of the cell cycle. Vorinostat also induces differentiation in the estrogen receptor-negative cell line SKBr-3 and the retinoblastoma-negative cell line MDA-468. [3] Vorinostat treatment at 1 μM for 8 hours or more is sufficient to irreversibly induce apoptosis of human multiple myeloma (MM) cells. The gene expression profiles of Vorinostat treated MM cells are not hallmarked by global transcriptional activation, but by coordinated transcriptional changes of specific functional groups of genes such as cytokine-induced proliferative/survival signaling cascades, oncogenes-tumor suppressor genes, regulators of apoptosis, DNA synthesis-repair and cell cycle, and proteasome-ubiquitin function. [4]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
H1299 NVLjb3VxU2mwYYPlJGF{e2G7 MW[yNEBucW5? NFLmfoxFVVOR Moe1TY5pcWKrdH;yfUBi[3Srdnn0fUBi\2GrboP0JGhFSUNiZX76fY1mKHerdHigTWM2OCCxZjCwMlE6PCEQvF2= M4nKXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF2NUKxOFIzLz5zNEWyNVQzOjxxYU6=
HT1080 MkLrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU[3NkBp MmKxSG1UVw>? NH64fphKSzVyPUKuOEDPxE1? NGrjNFY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zMkK3NFE4PSd-MUKyO|AyPzV:L3G+
K562 M1KyUGtqdmG|ZTDBd5NigQ>? NFTGeVQ3KGh? M37iXWROW09? MmHpTY5pcWKrdHnvckBw\iCqdX3hckBpcXO2b37lJIRm[WOndInsZZNmKCivaYj0eZJmKG:oIFjERWMyKGGwZDDISGFEOilid3n0bEBKSzVyIH;mJFAvODFizszN MknLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTJ2MUmzPFAoRjF{NEG5N|gxRC:jPh?=
A20 NVrZXlk6U2mwYYPlJGF{e2G7 MXyxJIg> M{XVUGROW09? Ml3xZY51cS2KRFHDNUBi[3Srdnn0fUB4cXSqIFnDOVAhd2ZiMD6xNVIh|ryP M4\hXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzFzOU[wOFg6Lz5zMUm2NFQ5QTxxYU6=
Friend leukemic cell NGTjboZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFnxbHU2OCEQvF2= NF3GV|U4OiCq M1HPUmROW09? MnPxTWM2OD1yLkm5JO69VQ>? MX:8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yOjFyOUmxN{c,OTJzMEm5NVM9N2F-
H1299 M{jw[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVS3NkBp Mnr5SG1UVw>? MYnJR|UxRTdwMkSg{txO MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPDV{MUSyNkc,OTR3MkG0NlI9N2F-
HeLa NUG4XGJrU2mwYYPlJGF{e2G7 MUKzNEBucW5? MXjJcohq[mm2b4L5JINwdmOnboTyZZRqd25iYXfhbY5{fCCqaYP0c45mKGSnYXPleJlt[XOnIIfpeIghUUN3MDDv[kAxNjJ6IN88US=> MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPTZyM{m0PUc,OTV4MEO5OFk9N2F-
LOX-IMVI M1Ozemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{PYWlQ5KGh? MXHJR|UxRTFwMzFOwG0> NETl[pM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNUexOVQ4OCd-MUW3NVU1PzB:L3G+
NCI-H226 NWXzbGx2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4[3e|Q5KGh? NHrOSFRKSzVyPUKuOkDPxE1? MlPqQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5MUW0O|AoRjF3N{G1OFcxRC:jPh?=
RXF-631L MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWC0PEBp M3ixUWlEPTB;MjFOwG0> M4PCb|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF3N{G1OFcxLz5zNUexOVQ4ODxxYU6=
SNB-78 NILnT5FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M17aXlQ5KGh? M3:wOWlEPTB;MU[g{txO MnjXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTV5MUW0O|AoRjF3N{G1OFcxRC:jPh?=
St-4 MoDIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVnMWlNsPDhiaB?= MkC2TWM2OD13LkKg{txO NWL0Unl3RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUW3NVU1PzBpPkG1O|E2PDdyPD;hQi=>
ARP1 Mo\3SpVv[3Srb36gRZN{[Xl? NV23dnplOiEQvF2= MWS2JIg> MofCTY5lfWO2aX;uJI9nKFB{MWfBSlEh\2WwZTDlfJBz\XO|aX;u NFXqVXM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zNEezOFgxPid-MUS3N|Q5ODZ:L3G+
CEM M3zUT2tqdmG|ZTDBd5NigQ>? NIfiNGkyKGh? NIjhUVdFVVOR M13FWmlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD6zN{DPxE1? MWS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPjR{MEC2OEc,OTZ2MkCwOlQ9N2F-
HCT15 NVXWSZE5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MU[0PEBp NGjte2pIUTVyPUCuPFIh|ryP M33HdlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4N{KzNlI4Lz5zNkeyN|IzPzxxYU6=
NCI-H23 NFfhTWZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWK0PEBp MVPHTVUxRTBwOUKg{txO Ml3hQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ5MkOyNlcoRjF4N{KzNlI4RC:jPh?=
GM15850 NI\JT5BMcW6jc3WgRZN{[Xl? NI\nblgzNjVizszN MWq5OkBp NYjIVHAyTE2VTx?= Mn;PTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViSEOgcJl{cW6nIEG0JIFk\XS7bHH0bY9vKG:oIF\YUkBo\W6n MnfGQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7MkGzOlcoRjF4OUKxN|Y4RC:jPh?=
GM15850 MXnLbY5ie2ViQYPzZZk> NHjye5ozNjVizszN MkT4PVYhcA>? MV;EUXNQ MX3Jcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKNDDsfZNqdmViNTDhZ4V1gWyjdHnvckBw\iCIWF6g[4Vv\Q>? NYTkO|RMRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMU[5NlE{PjdpPkG2PVIyOzZ5PD;hQi=>
GM15850 NWfmbXNvU2mwYYPlJGF{e2G7 Ml:1Nk42KM7:TR?= NUHpSmtkQTZiaB?= MlfVSG1UVw>? NX\2UHd6UW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFQhdHm|aX7lJFEzKGGlZYT5cIF1cW:wIH;mJGZZViCpZX7l M3LnPVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUKxN|Y4Lz5zNkmyNVM3PzxxYU6=
GM15850 MUHLbY5ie2ViQYPzZZk> NFn5WokzNjVizszN NHHkdWE6PiCq NHP2eWNFVVOR MVrJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCKMzDsfZNqdmViOTDhZ4V1gWyjdHnvckBw\iCIWF6g[4Vv\Q>? MlrXQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTZ7MkGzOlcoRjF4OUKxN|Y4RC:jPh?=
GM15850 NHO5PXJMcW6jc3WgRZN{[Xl? M3nr[VIvPSEQvF2= M1TkNlk3KGh? MVLEUXNQ Mm[wTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHjpd5RwdmViSESgcJl{cW6nIEigZYNmfHmuYYTpc44hd2ZiRmjOJIdmdmV? M2TMXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUKxN|Y4Lz5zNkmyNVM3PzxxYU6=
GM15850 MUfLbY5ie2ViQYPzZZk> NH\mcXAzNjVizszN M37jeFk3KGh? NYfEfYt6TE2VTx?= NH3VSGpKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6gbIl{fG:wZTDIOEBtgXOrbnWgNVYh[WOndInsZZRqd25ib3[gSnhPKGenbnW= M1HkR|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF4OUKxN|Y4Lz5zNkmyNVM3PzxxYU6=
U937 MorkSpVv[3Srb36gRZN{[Xl? M{jEe|Uh|ryP NVvSWWZGOjRiaB?= MX7EUXNQ NYm4SXlqS2WubDDjfYNt\SCjcoLld5Qh[nliYXPjeY12dGG2aX;uJIF1KFNxR{KvUUBxcGG|ZR?= NGTjdnE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{CwOFcyQCd-MUewNFQ4OTh:L3G+
H661 NIfXeZRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{\0[VQ5KGh? NYTRWIlMUUN3ME2xJO69VQ>? NH;Xcms9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zN{G1O|AxQSd-MUexOVcxODl:L3G+
U937 M{ntNmZ2dmO2aX;uJGF{e2G7 NWPrZVNbPSEQvF2= NFn1dYczPCCq M4TsUmROW09? NWfLcW94UW6lcnXhd4UhcW5iaHnzeI9v\SCKMzDhZ4V1gWyjdHnvci=> M3\UOlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NES3O|UxLz5zN{S0O|c2ODxxYU6=
U937 MY\GeY5kfGmxbjDBd5NigQ>? M{nhZlEh|ryP NYK0UHA5OjRiaB?= NIftc5dFVVOR NHGyUIdG\m[nY4Sgc44h[WyyaHGgeJVjfWyrbjDhZ4V1gWyjdHnvci=> M12wZ|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NES3O|UxLz5zN{S0O|c2ODxxYU6=
RAW264.7 MVXGeY5kfGmxbjDBd5NigQ>? MVGxNEDPxE1? MVOyOIg> M{\odZdifGW{ M2HzPGlvcGmkaYTpc44hd2ZiTGDTMZN1cW23bHH0[YQhXE6IYXzwbIEheHKxZIXjeIlwdiC5aYToJGlEPTBib3[gNE44PiEQvF2= NVTsWYxzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe0O|c2OThpPkG3OFc4PTF6PD;hQi=>
RAW264.7 M{TlPWZ2dmO2aX;uJGF{e2G7 NGPGV5gyOCEQvF2= MV[yOIg> NUXMeVdpf2G2ZYK= MYjJcohq[mm2aX;uJI9nKEySUz3zeIlufWyjdHXkJG5QKHC{b3T1Z5Rqd25id3n0bEBKSzVyIH;mJFEvQDhizszN M2W1TFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NEe3OVE5Lz5zN{S3O|UyQDxxYU6=
SC9 M1\T[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1[5V|Q5KGh? Mk\wTWM2OD1yLk[wOkDPxE1? MWq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzVyN{KxPUc,OTd3MEeyNVk9N2F-
ST14A M3TReGZ2dmO2aX;uJGF{e2G7 Mn3COUBvVQ>? MmmzO|IhcA>? M{DrPGlv[3KnYYPlJIlvKFKHU2SvUnJUTiCvUl7BJIV5eHKnc4Ppc44> MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8yPzV4NUm5N{c,OTd3NkW5PVM9N2F-
ST14A Ml3BSpVv[3Srb36gRZN{[Xl? NHzScIg2KG6P M4PCPFczKGh? MWrJcoNz\WG|ZTDpckBGOkZzIH3SUmEh\XiycnXzd4lwdg>? Mk\xQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd3NkW5PVMoRjF5NU[1PVk{RC:jPh?=
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PANC1 MV\GeY5kfGmxbjDBd5NigQ>? NHnKbosyKM7:TR?= NW\WVJV2OjRiaB?= MYLJcoNz\WG|ZTDpckBxOjetaYCxJIdmdmViZYjwdoV{e2mxbh?= NWDm[XpvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMUe1PFg4PDRpPkG3OVg5PzR2PD;hQi=>
PT45 NY\neYhoT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkjlTWM2OD12IN88US=> M1nHXFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzF5NUi4O|Q1Lz5zN{W4PFc1PDxxYU6=
ACHN M4LyRmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2C2VFIyKGh? NWTSdHh2T0l3ME2wMlY2KM7:TR?= MmXEQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTd4NkK2NFYoRjF5Nk[yOlA3RC:jPh?=
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Primary bronchial epithelial cell NEO5RVlMcW6jc3WgRZN{[Xl? MVO1JO69VQ>? MVmyOEBp NEXiU2xFVVOR NGD3TJlKdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJI12fGGwdDDG[IVtfGF3MEigR2ZVWiCycn;0[YlvKGGyaXPhcEB{fXKoYXPlJIxw[2GuaYrheIlwdg>? MnTQQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOTl7Nk[3PFkoRjF7OU[2O|g6RC:jPh?=
Primary bronchial epithelial cell MXfLbY5ie2ViQYPzZZk> NWnFRY41OSEQvF2= M4G2elk3KGh? MUPEUXNQ NH;NZVJKdmirYnn0bY9vKG:oIFjERWM4KGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJJNpd3K2IHPpdoN2cXRiY4XydoVvfHN? NIHBTmc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9zOUm2Olc5QSd-MUm5OlY4QDl:L3G+
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CAL27 M334WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGS4UWU4OiCq M{TDTWlEPTB;Mz63NkDPxE1? NFT6TYo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEG0N|c4QCd-MkCxOFM4Pzh:L3G+
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NCI-H2122 NV7ZdWtET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1:xWlczKGh? M1q2RWlEPTB;Nz61JO69VQ>? MknBQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO3O|goRjJyMUSzO|c5RC:jPh?=
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SKHEP1 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;Jfm04OiCq M3;qZmlEPTB;Mz60OkDPxE1? MVm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{e3PEc,OjBzNEO3O|g9N2F-
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Caco-2 MlfMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2\oTFExOCEQvF2= NGnrRpg1QGh? NVTJeYV2TE2VTx?= NX\vNIV2UUN3ME24MlEh|ryP MYe8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
Caco-2 NHy0b2RMcW6jc3WgRZN{[Xl? NGHsPFA2KM7:TR?= MmHDOEBp MULEUXNQ NXXrZndiUW6mdXP0bY9vKG:oIFjERWMudWWmaXH0[YQhcGm|dH;u[UBi[2W2eXzheIlwdiCLQ{WwJI9nKDBwMEO3JO69VQ>? MVi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
Caco-2 M2HIfmtqdmG|ZTDBd5NigQ>? NVn4cJlnOC5|MU[gcm0hfG9iM{GuOkDPxE1? NX3MOZlzPCCq M1fSO2ROW09? Mk\aSZghfmm4bzDpcohq[mm2aX;uJI9nKGi3bXHuJGhFSUNzIIfpeIghUUN3MDDv[kAxNjFzOTFOwG0> NVuyVFBzRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
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Caco-2 MmmzT4lv[XOnIFHzd4F6 MmDUOk4{OiCwTTD0c{A3OzJizszN Mk\NOEBp NHezSZNFVVOR NFjFdZNGgCC4aY\vJIlvcGmkaYTpc44hd2ZiaIXtZY4hUESDQ{ege4l1cCCLQ{WwJI9nKDRzIN88US=> MXK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
Caco-2 MYXLbY5ie2ViQYPzZZk> MVywMlMyPiCwTTD0c{A{OS54IN88US=> MWi0JIg> M364SWROW09? MVXFfEB3cX[xIHnubIljcXSrb36gc4YhcHWvYX6gTGRCSzZ? Mo\sQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjBzNEO4OFAoRjJyMUSzPFQxRC:jPh?=
HepG2 NEDyVXhMcW6jc3WgRZN{[Xl? Ml\qOUDPxE1? NEjhNZM3cA>? NF;WRZBFVVOR MnXDTY5lfWO2aX;uJI9nKEiGQVOtcYVlcWG2ZXSgbIl{fG:wZTDhZ4V1gWyjdHnvci=> MXS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
HepG2 NVPmVpVyU2mwYYPlJGF{e2G7 NXTsVm1HPSEQvF2= NH\UOlU3cA>? MUjEUXNQ M3y0eGlv\HWldHnvckBw\iCKRFHDMY1m\GmjdHXkJIhqe3SxbnWgbJlx\XKjY3X0fYxifGmxbh?= MV28ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
HepG2 MmXuT4lv[XOnIFHzd4F6 MnLlOUDPxE1? NV;CeoJYPmh? NFewW41FVVOR NYGzc5dEUW6mdXP0bY9vKG:oIFjERWMudWWmaXH0[YQh[WyyaHGteJVjfWyrbjDhZ4V1gWyjdHnvci=> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODF2M{i0NEc,OjBzNEO4OFA9N2F-
HepG2 NXPzNGoyU2mwYYPlJGF{e2G7 MWi1JO69VQ>? MYG2bC=> NYS4UoRlTE2VTx?= NYj4VG5nUW6mdXP0bY9vKG:oIFjERWMudWWmaXH0[YQhUDNxSESgbIl{fG:wZTDhZ4V1gWyjdHnvci=> NWK0dWFPRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkCxOFM5PDBpPkKwNVQ{QDRyPD;hQi=>
COLO205 NEjDTWVMcW6jc3WgRZN{[Xl? NWrFS4ZlOTBizszN NG\HOlczPCCq MoLZSG1UVw>? M{HwNGlvcGmkaYTpc44hd2ZiSFTBR|Eh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIhqe3SxbnWgTFMh[WOndInsZZRqd25id3n0bEBKSzVyIH;mJFIvOiEQvF2= NF3XNXE9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES1NVM4QCd-MkC0OVE{Pzh:L3G+
SCC4 M2X3V2tqdmG|ZTDBd5NigQ>? NGX6U3IyKM7:TR?= MnnpNlRp NFTveI9KdmirYnn0bY9vKG:oIFjERWMh[XO|ZYPz[YQh[XNiaX7jdoVie2ViaX6geJVjfWyrbjDhZ4V1gWyjdHnvci=> M4LnUVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEWyNlI2Lz5{MES1NlIzPTxxYU6=
SCC4 M1H2Z2tqdmG|ZTDBd5NigQ>? NHL3eW8yKM7:TR?= MlzmNlRp MWrJcohq[mm2aX;uJI9nKEiGQVOgZZN{\XO|ZXSgZZMhcW6lcnXhd4UhcW5iaHnzeI9v\SCjY3X0fYxifGmxbh?= NHvtOYY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES1NlIzPSd-MkC0OVIzOjV:L3G+
SCC4 MlLESpVv[3Srb36gRZN{[Xl? MWWxJO69VQ>? NGjGb2w2KG2rbh?= NETLNZNC\2:waYP0JIFkfGm4aYT5JIF1KF[GUjDhd5Nme3OnZDDhd{BqdmS3Y4Tpc44hd2ZiQ2nQNlQh\2WwZTDlfJBz\XO|aX;u MX[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zODR3MkKyOUc,OjB2NUKyNlU9N2F-
NB4 NEPMR2NHfW6ldHnvckBCe3OjeR?= MoT4OUDPxE1? M1XablI1KGh? M{LuSGROW09? NHvTZmNE\WyuIHP5Z4xmKGG{cnXzeEBie3Onc4Pl[EBieyCrbnPy[YF{\SCrbjDhZ4N2dXWuYYTpc44h[XRicILlMWcyKHCqYYPl MkXJQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjB2OUG0OFAoRjJyNEmxOFQxRC:jPh?=
NB4 NI\6cGtMcW6jc3WgRZN{[Xl? MUS1JO69VQ>? M1nwNFI1KGh? NXqze|JJTE2VTx?= MmHSTY5pcWKrdHnvckBw\iCKRFHDJIF{e2W|c3XkJIF{KGmwY4LlZZNmKGmwIHHj[ZR6dGG2ZTD0eYJ2dGmwIHzleoVt M3LaTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEmxOFQxLz5{MES5NVQ1ODxxYU6=
NB4 MnjZSpVv[3Srb36gRZN{[Xl? M2i2XlUh|ryP NFPBUpAzPCCq NH3EUW1FVVOR NYL2fVhNUW6mdXP0bY9vKG:oIICyNXdCTjFxQ1nQNUBmgHC{ZYPzbY9v M4DobVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJyNEmxOFQxLz5{MES5NVQ1ODxxYU6=
NB4 M3O0[GtqdmG|ZTDBd5NigQ>? MYS1JO69VQ>? MkHUNlQhcA>? M4LtfmROW09? NYDjRWRtUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IHnuZ5Jm[XOnIHnuJIhqe3SxbnWgTFMh[WOndInsZZRqd25? NHL6cok9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MES5NVQ1OCd-MkC0PVE1PDB:L3G+
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A549 MonGSpVv[3Srb36gRZN{[Xl? MWS1JO69VQ>? NEXOOnI{KGh? NYXyXYpWTE2VTx?= M{HVVmlv\HWldHnvckBw\iCjdYTvdIhi\3liaX6gbJVu[W5iQUW0PUBk\WyuczDhd5Nme3OnZDDhd{BqdmO{ZXHz[UBqdiCOQ{PJTS=> NGnJSYM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi1OVIxQCd-MkC4OVUzODh:L3G+
HUT78 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXW3NkBp MVrEUXNQ MWLHTVUxRTBwMzFOwG0> NHOzfHo9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MEi4OFIxQCd-MkC4PFQzODh:L3G+
U937 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1f1blczKGh? MYXEUXNQ NUTkSlNxT0l3ME2wMlM6KM7:TR?= NXnMTnY2RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkC4PFQzODhpPkKwPFg1OjB6PD;hQi=>
A431 M2PBemN6fG:2b4jpZ{BCe3OjeR?= M3\wUFczKGh? M2W2eWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGE1OzFiY3XscJMhd3[ncnX4dJJme3OrbnegSWdHWiC5aYToJGlEPTBib3[gOE4zKM7:TR?= MUi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[yPUc,OjFyOEC2Nlk9N2F-
A549 M3HFTGN6fG:2b4jpZ{BCe3OjeR?= NVjhZZlNPzJiaB?= MlzLR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gRVU1QSClZXzsd{BwfmW{ZYjwdoV{e2mwZzDISGFEKHerdHigTWM2OCCxZjCxMlgh|ryP NWXmWGFLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3OjlpPkKxNFgxPjJ7PD;hQi=>
CAL27 NXvCOVNjS3m2b4TvfIlkKEG|c3H5 NYXQdWJiPzJiaB?= MkLJR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gR2FNOjdiY3XscJMhd3[ncnX4dJJme3OrbnegSWdHWiC5aYToJGlEPTBib3[gN{4zKM7:TR?= NWPjNpZvRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkGwPFA3OjlpPkKxNFgxPjJ7PD;hQi=>
HeLa MmDwR5l1d3SxeHnjJGF{e2G7 MV[3NkBp Ml\wR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTIVN[SClZXzsd{BwfmW{ZYjwdoV{e2mwZzDISGFEKHerdHigTWM2OCCxZjCxMlUh|ryP MlPhQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2NlkoRjJzMEiwOlI6RC:jPh?=
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SKBR3 NVfVW3BjS3m2b4TvfIlkKEG|c3H5 MnHQO|IhcA>? Mo\pR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2tDWjNiY3XscJMhd3[ncnX4dJJme3OrbnegTGVTOiC5aYToJGlEPTBib3[gNk43KM7:TR?= MlP2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2NlkoRjJzMEiwOlI6RC:jPh?=
SKOV3 NFr4XXJEgXSxdH;4bYMhSXO|YYm= NGHBZnc4OiCq Mn;WR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gV2tQXjNiY3XscJMhd3[ncnX4dJJme3OrbnegTGVTOiC5aYToJGlEPTBib3[gNk4zKM7:TR?= NFvYdoQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFYzQSd-MkGwPFA3Ojl:L3G+
COR-L23 NF;HeYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV7DNG11OTBizszN NFf5U4U4OiCq NHrYRnRFVVOR M4SyZmdKPTB;MT6xJO69VQ>? MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[0O{c,OjFyOEC2OFc9N2F-
DU145 M3H4T2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV2xNEDPxE1? M3O4fFczKGh? NH\WSGRFVVOR NXS2blkzT0l3ME2xMlA2KM7:TR?= NHiydIc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFY1Pyd-MkGwPFA3PDd:L3G+
LoVo MlfOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rKUFExKM7:TR?= M33kdVczKGh? M3jYTmROW09? NUnre2psT0l3ME2xMlch|ryP NGS2emU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFY1Pyd-MkGwPFA3PDd:L3G+
MCR5-SV2 MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M130OFExKM7:TR?= M{X3NVczKGh? MoXKSG1UVw>? NHLFT3FIUTVyPUKuNUDPxE1? MW[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOTB6ME[0O{c,OjFyOEC2OFc9N2F-
HCT116 M2TrUWtqdmG|ZTDBd5NigQ>? MWGzNEDPxE1? NVnifppYOzBiaB?= MWHEUXNQ NHO1S5BKdmirYnn0bY9vKG:oIFjERWM3KGG|c3Xzd4VlKGG|IHnu[JVkfGmxbjDv[kBidHCqYT30eYJ2dGmwIHHj[ZR6dGG2aX;u NETRW|c9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MUC4NFY1Pyd-MkGwPFA3PDd:L3G+
HCT116 M2PkNWtqdmG|ZTDBd5NigQ>? NXvWWVVkOzBizszN MWGzNEBp MYnEUXNQ M2nVW2lvcGmkaYTpc44hd2ZiSFTBR|Eh[XO|ZYPz[YQh[XNiaX7keYN1cW:wIH;mJIhqe3SxbnWgTFNMQSCjY3X0fYxifGmxbh?= MkjYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjFyOEC2OFcoRjJzMEiwOlQ4RC:jPh?=
U937 NXTHPFN7U2mwYYPlJGF{e2G7 M2TZSlUh|ryP MkPSNlQhcA>? MlnHSG1UVw>? MUjJcohq[mm2aX;uJI9nKGirc4TvcoUh[WOndInseJJidnOoZYLhd4Uh[XO|ZYPz[YQh[XNiaX7jdoVie2Vib3[gZYNmfHmuYYTpc44hd2ZiaHnzeI9v\SCKMzDheEBMQQ>? M2XYV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmyOFkzLz5{MUK5NlQ6OjxxYU6=
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U937 M{XLNWtqdmG|ZTDBd5NigQ>? MWm1JO69VQ>? NFTh[IMyPSCvaX6= MWPEUXNQ NGfzTZlKdmirYnn0bY9vKG:oIHjpd5RwdmViYXPleJltfHKjboPm[ZJie2ViYYPz[ZN{\WRiYYOgbY5kemWjc3Wgc4YhcGm|dH;u[UBJOyCjY3X0fYxifGmxbjDs[ZZmdA>? M2TvV|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJzMkmyOFkzLz5{MUK5NlQ6OjxxYU6=
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COLO205 NUPzWnpyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Ml7UPVYhcA>? NHn3VVRFVVOR MUXJR|UxRTJwMUKg{txO MmHVQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF4M{S0N|AoRjJzNkO0OFMxRC:jPh?=
Hep3B MnTwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXy4eWI1PDhiaB?= MmTpSG1UVw>? NWnWPJRxT0l3ME2xMlIyKM7:TR?= NW[wfIk6RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG3NVIyPDZpPkKxO|EzOTR4PD;hQi=>
GM11272 NY\vZ|dNTnWwY4Tpc44hSXO|YYm= MV6xNFAhdk1? MlT1O|IhcA>? M4LTRmROW09? MXrS[YFkfGm4YYTpc44hd2ZiTXXDdFIhdXW2YX70JIV5eHKnc4Ppc44h[XRiMUCgeI8hOTByIH7N NX;L[3pXRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG4OFA4OTZpPkKxPFQxPzF4PD;hQi=>
CFBE41o M1PtTGtqdmG|ZTDBd5NigQ>? M1XLTFUh|ryP NFzyUmVFVVOR Mn:5SI94diC{ZXf1cIF1cW:wIH;mJGhFSUN5IHH0JFAvOiC2bzC1JJVO NVjlepZ5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkG5PFQ6PThpPkKxPVg1QTV6PD;hQi=>
CFBE41o MVnGeY5kfGmxbjDBd5NigQ>? M3HHeFEh|ryP M{DzRVI1KGh? MUXEUXNQ MljJVoV{fG:{YYTpc44hd2ZiQ1\UVkBl\Wy2YV[1NFghdXW2YX70JJRz[W[oaXPrbY5oKHSxIHPlcIwhe3W{ZnHj[UBieyCpbInjc4Zwem2| MmXiQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjF7OES5OVgoRjJzOUi0PVU5RC:jPh?=
DU145 NV\3S2pkU2mwYYPlJGF{e2G7 MY[yMlUh|ryP M{\W[FI1KGh? M{izcWROW09? NXmyVJhvUW6qaXLpeIlwdiCxZjDISGFEKGG|c3Xzd4VlKGG|IIXwdoVofWyjdHnvckBw\iCyMkH3ZYYyKHC{b4TlbY4h\XiycnXzd4lwdg>? MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ4MEG2Okc,OjJ{NkCxOlY9N2F-
DU145 NUHETIpiU2mwYYPlJGF{e2G7 NUPmZ2VNPSEQvF2= MVqyOEBp M3T4RmROW09? M4\qOmlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCqaYP0c45mKEh2IHHj[ZR6dGG2aX;u MYO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjJ4MEG2Okc,OjJ{NkCxOlY9N2F-
DU145 M4HKNGtqdmG|ZTDBd5NigQ>? MWO1JO69VQ>? MVyyOEBp MnrkSG1UVw>? M4S0dmlvcGmkaYTpc44hd2ZiSFTBR{Bie3Onc4Pl[EBieyCqaYP0c45mKEh|IHj5dIVz[WOndInsZZRqd25? M3PZWFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{Mk[wNVY3Lz5{MkK2NFE3PjxxYU6=
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A498 M1vEOmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3y1ZVYvOiEQvF2= MnTFO|IhcA>? MX7EUXNQ M4fWd2dKPTB;MT62JO69VQ>? M3\FVFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
A549/ATCC Ml3XS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoDMOk4zKM7:TR?= Moi3O|IhcA>? NFewR3pFVVOR MUfHTVUxRTFwNjFOwG0> MkPLQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
ACHN NF25d3lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV22MlIh|ryP MlHqO|IhcA>? NGC5c45FVVOR M4HvNWdKPTB;MT6zJO69VQ>? MnXMQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
BT549 M3LpcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVW0OYFnPi5{IN88US=> NH\TZ4c4OiCq NXXnV242TE2VTx?= Mo[yS2k2OD1zLkOg{txO M2jURVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
EKVX NGnLdG9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWmx[4JbPi5{IN88US=> M{fXSVczKGh? M2DXPWROW09? MVrHTVUxRTFwMzFOwG0> NWfrVnRIRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
HCT15 M{jxWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M12xN|YvOiEQvF2= NULxfJBPPzJiaB?= NHvkUmVFVVOR M4PNWWdKPTB;Mj61JO69VQ>? Mmm2QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
HOP92 M{HBbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M33EUlYvOiEQvF2= NFzmNWI4OiCq NUfsbmR[TE2VTx?= MYDHTVUxRTRizszN NVzXVmdjRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
IGROV1 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1LvNlYvOiEQvF2= MmTHO|IhcA>? M2f4UWROW09? MojxS2k2OD1zLkOg{txO MmnvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
LOXIMVI NXzldGdlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3LVWlYvOiEQvF2= NHfYRXg4OiCq MW\EUXNQ MXvHTVUxRTFizszN NEfEe|g9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
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MALME-3M M1i0NWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\WVoprPi5{IN88US=> NWDISWVUPzJiaB?= MU\EUXNQ NIP1cJVIUTVyPUCuN|Ih|ryP MmXWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
MCF10A MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmrXOk4zKM7:TR?= MnHaOFghcA>? Ml\xSG1UVw>? M{\vbGdKPTB;OT62JO69VQ>? NFvKNWc9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
MDA-MB-435 MoS5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M320NlYvOiEQvF2= MWe3NkBp NUW5d|ZGTE2VTx?= MnzKS2k2OD1yLkWg{txO NHXJWZM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
MDA-MB-468 M1HMTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfHOJg3NjJizszN MknnO|IhcA>? MWrEUXNQ MnXpS2k2OD1yLk[zJO69VQ>? M3vWS|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
MOLT4 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XJVlYvOiEQvF2= NYPBRpN{PzJiaB?= NU\CNXgzTE2VTx?= MlLtS2k2OD1yLkSg{txO M3PYeVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
NCI-ADR-RES NYXHOFBKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnzUOk4zKM7:TR?= MX[3NkBp M1vCdWROW09? NX72[Xk2T0l3ME2wMlE3KM7:TR?= NHyyfYU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
NCI-H23 NIPu[XVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2LuelYvOiEQvF2= NYDVTWpVPzJiaB?= NFLIUotFVVOR M2fCN2dKPTB;MTFOwG0> MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
NCI-H322M NV\rN29PT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEjEU4o3NjJizszN NWXMbI9uPzJiaB?= NUfBTXlxTE2VTx?= NWXxUGtoT0l3ME2wMlc6KM7:TR?= NF3TfmM9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
NCI-H460 NXnnWIs1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUTCdGF2Pi5{IN88US=> NGTGVZU4OiCq MlTYSG1UVw>? NITJ[2hIUTVyPUCuO|kh|ryP Mlr5QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
NCI-H522 NGX2fotIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVi2MlIh|ryP NGHzNJk4OiCq MYTEUXNQ M2TLeWdKPTB;MD61JO69VQ>? MnmwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
OVCAR3 NXO0d|M1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF;JbVU3NjJizszN Mlf1O|IhcA>? NFXlbIpFVVOR M3m2XGdKPTB;MT6zJO69VQ>? NGi5cW49[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
OVCAR4 Mk\lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVry[Yc2Pi5{IN88US=> NX3BZoxYPzJiaB?= NWHBW451TE2VTx?= MWLHTVUxRTRizszN NVSw[oJERGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
OVCAR5 MoLxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3rXdVYvOiEQvF2= MnHrO|IhcA>? NUDUWHd[TE2VTx?= MVvHTVUxRTBwN{mg{txO NGfQUFg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
OVCAR8 NFnm[ZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIrJ[XU3NjJizszN M1jSWVczKGh? M2PkemROW09? MX\HTVUxRTBwNTFOwG0> NGG2NnQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
RXF393 NVGwNVJ7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;IUFYvOiEQvF2= M4Dp[|czKGh? MkCySG1UVw>? NXfvOml3T0l3ME2xMlMh|ryP NXrYSmZ1RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
SF268 MorjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHrK[JQ3NjJizszN MnHaO|IhcA>? NHLHUHBFVVOR M{jjU2dKPTB;MT62JO69VQ>? MkDDQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
SF295 NXvVRVdVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3HPN|YvOiEQvF2= M{XNUFczKGh? M2PJbWROW09? MVzHTVUxRTFwNjFOwG0> MWW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
SF539 NHPtco5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHv0UZU3NjJizszN M2frU|czKGh? NFvBVFJFVVOR MXfHTVUxRTJizszN NVrad4lLRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
SK-MEL-2 M2WySWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYfndYloPi5{IN88US=> NYWyW|Z[PzJiaB?= Ml3KSG1UVw>? NFryfoZIUTVyPUGuN{DPxE1? NFKxO3E9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
SK-MEL-28 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYm5WmgyPi5{IN88US=> NWD1OpJTPzJiaB?= NUPtUJFPTE2VTx?= M1zufWdKPTB;MTFOwG0> NHflfWQ9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
SK-MEL-5 NVrlPVR2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\TdFYvOiEQvF2= NVvxd5JiPzJiaB?= M{LsVWROW09? MUnHTVUxRTBwNkOg{txO NILEOVY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
SKOV3 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGe5N|Y3NjJizszN NF3RO2g4OiCq MWXEUXNQ MXLHTVUxRTFizszN M1TL[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
SN12C NVe2Wm1MT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVvSPWloPi5{IN88US=> NYW1SY5QPzJiaB?= MYXEUXNQ NEDvcnpIUTVyPUKg{txO M2HhSlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
SNB19 MnTSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MljKOk4zKM7:TR?= MnLFO|IhcA>? M1TuSWROW09? M1TOd2dKPTB;MD62N{DPxE1? MlvoQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjJ2M{W2OlkoRjJ{NEO1OlY6RC:jPh?=
SNB75 MoG4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX;4cYQyPi5{IN88US=> MkX5O|IhcA>? M4TPW2ROW09? NU\4T5pGT0l3ME2wMlc6KM7:TR?= NI\iZYI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
SR M3\rbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{\JPFYvOiEQvF2= NX7ze3FmPzJiaB?= MV7EUXNQ NY\lSlhuT0l3ME2wMlQh|ryP M13kTlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
T47D M4[xeWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGL4d5Y3NjJizszN M3rlPFczKGh? NHrCN5lFVVOR MmDMS2k2OD1yLkWg{txO MUO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
TK10 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M13nS|YvOiEQvF2= MVG3NkBp M2HFeWROW09? NFnaVnBIUTVyPUCuOlMh|ryP NELjNXU9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{MkSzOVY3QSd-MkK0N|U3Pjl:L3G+
UACC257 NVrLZ45iT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\jbVYvOiEQvF2= NVrsZYs{PzJiaB?= M3TwZ2ROW09? MXXHTVUxRTBwNTFOwG0> M4\HXlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ{NEO1OlY6Lz5{MkSzOVY3QTxxYU6=
UACC62 M3zyTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVe2MlIh|ryP NHvCTYs4OiCq NUSyZXNETE2VTx?= NUPOd5JET0l3ME2wMlQh|ryP MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|NU[2PUc,OjJ2M{W2Olk9N2F-
UO31 MlTxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVu2MlIh|ryP NGTEc5I4OiCq M4f2fGROW09? MVfHTVUxRTBwNTFOwG0> NXrPe5dwRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkK0N|U3PjlpPkKyOFM2PjZ7PD;hQi=>
Hep3B M3nwS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4PVOVQ5KGh? MkfxSG1UVw>? NU\uWmR6T0l3ME2wMlY6KM7:TR?= MVq8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjR|OUi2N{c,OjJ2M{m4OlM9N2F-
BE(2)-C NYnkflR[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MV23NkBp NVfYdmRTTE2VTx?= M2HCeWlEPzB;MjFOwG0> MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOjl|MkOxOkc,OjJ7M{KzNVY9N2F-
Bel7402 MkLVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYH3Zll6PzJiaB?= MVvEUXNQ MkLQS2k6OD1zNzFOwG0> NVH1ZW15RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOwOlE{PzZpPkKzNFYyOzd4PD;hQi=>
Bel7404 MnO1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWPSTmw4PzJiaB?= M33wVWROW09? NIrrU49IUTlyPUG4JO69VQ>? MWC8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzB4MUO3Okc,OjNyNkGzO|Y9N2F-
BGC823 NVL3[2dUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3mwUFczKGh? MkDmSG1UVw>? NWLCcVVvT0l7ME23MlUh|ryP M2DENlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|ME[xN|c3Lz5{M{C2NVM4PjxxYU6=
MGC803 NVq4cYhtT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUnrW|JRPzJiaB?= M1jSPWROW09? NFHlU3RIUTlyPUGwMlgh|ryP MmLYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjNyNkGzO|YoRjJ|ME[xN|c3RC:jPh?=
SGC7901 MXPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYW3NkBp NIrW[|hFVVOR Mm\kS2k6OD17LkSg{txO M1rTbFxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|ME[xN|c3Lz5{M{C2NVM4PjxxYU6=
SKHEP1 M2O4N2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{nLV|czKGh? M3foemROW09? MVLHTVkxRTF5IN88US=> NETpPG89[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{C2NVM4Pid-MkOwOlE{PzZ:L3G+
SMMC7721 NFToe3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUP4ZXdqPzJiaB?= MULEUXNQ MkDVS2k6OD1{MDFOwG0> M3nhZlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|ME[xN|c3Lz5{M{C2NVM4PjxxYU6=
NUGC3 NVTWXXBGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4jBelExKM7:TR?= M3S5XGdKRTJwOUSg{txO MXy8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzF4M{OzNkc,OjNzNkOzN|I9N2F-
A2780 CisR M3rXbmtqdmG|ZTDBd5NigQ>? NFG1e5gyQCCq NUnhUplqUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlQ1KM7:TR?= MYW8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
CAL27 sens NFS3T4FMcW6jc3WgRZN{[Xl? MXqxPEBp MXHJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwM{[g{txO NXrXRndORGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
CAL27 CisR MYTLbY5ie2ViQYPzZZk> NHTsdGsyQCCq NVXFRmFNUW6qaXLpeIlwdiCxZjDISGFEKHerdHigTWM2OCCxZjCwMlYyKM7:TR?= MWK8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
KYSE-510 CisR MVnLbY5ie2ViQYPzZZk> MUixPEBp M1LlXmlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD61PUDPxE1? NWKzVVNbRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
KYSE-510 sens Mn\mT4lv[XOnIFHzd4F6 MVexPEBp MVXJcohq[mm2aX;uJI9nKEiGQVOge4l1cCCLQ{WwJI9nKDBwNzFOwG0> NUPC[5NZRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
MDA-MB-231 sens NFPwSZpMcW6jc3WgRZN{[Xl? NVfTcIFwOThiaB?= M1\tTGlvcGmkaYTpc44hd2ZiSFTBR{B4cXSqIFnDOVAhd2ZiMD62NUDPxE1? NWP5bJA4RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkOyOVI3ODNpPkKzNlUzPjB|PD;hQi=>
MDA-MB-231 CisR MXfLbY5ie2ViQYPzZZk> M33MSlE5KGh? MkTyTY5pcWKrdHnvckBw\iCKRFHDJJdqfGhiSVO1NEBw\iByLk[xJO69VQ>? MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzJ3Mk[wN{c,OjN{NUK2NFM9N2F-
ES2 NFLQTGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYS0PEBp NGCwbY1FVVOR M4O5TWlEPTB;MUKuO{DPxE1? MWO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzR7M{S0PUc,OjN2OUO0OFk9N2F-
MDA-MB-231 M1T1dGtqdmG|ZTDBd5NigQ>? NU\ueZh{OTBizszN Mlz1N|AhdWmw NIq2R2pFVVOR MX;Jcohq[mm2aX;uJI9nKEiGQVOyZS=> NEfrZ5A9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{S5N|Q1QSd-MkO0PVM1PDl:L3G+
MDA-MB-231 NH6yWHZMcW6jc3WgRZN{[Xl? MWqxJO69VQ>? M2XHNVI1KGh? M2DoVWROW09? MnvCTY5pcWKrdHnvckBw\iCKRFHDOkBie3Onc4Pl[EBieyCjY3X0fYxifGmxbjDs[ZZmdHNib3[geJVjfWyrbh?= NYnJWFBoRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO0PVM1PDlpPkKzOFk{PDR7PD;hQi=>
HS68 NUjKN|ZOS3m2b4TvfIlkKEG|c3H5 NVjFTnBNOTBizszN Mne1O|IhcA>? M2jZU2ROW09? MkXXTWM2OD12Lk[g{txO M3PROlxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NUewOVQzLz5{M{W3NFU1OjxxYU6=
MEF NGS1cm5EgXSxdH;4bYMhSXO|YYm= M2niWlExKM7:TR?= NVzPOVJGPzJiaB?= NF7RS3RFVVOR MoTZTWM2OD12LkSg{txO NVnXV5M5RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO1O|A2PDJpPkKzOVcxPTR{PD;hQi=>
BGC823 MmOxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmXWSG1UVw>? NHPTT5FIUTVyPUOuNFkh|ryP NETnSVI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[wNVcxPid-MkO2NFE4ODZ:L3G+
High5 NHzDUWZMcW6jc3WgRZN{[Xl? M332TFI1KGh? MkO2TY5pcWKrdHnvckBw\iCqdX3hckBz\WOxbXLpcoFvfCCKRFHDOkB4cXSqIFnDOVAhd2ZiMD6wPUDPxE1? M32xe|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkCyOVI{Lz5{M{[wNlUzOzxxYU6=
High5 NYi3W5NIU2mwYYPlJGF{e2G7 NU\TfnJlOjRiaB?= M{W5dmlvcGmkaYTpc44hd2ZiaIXtZY4hemWlb33ibY5idnRiSFTBR|Ehf2m2aDDJR|UxKG:oIECuNVIh|ryP NVPFWIhHRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2NFI2OjNpPkKzOlAzPTJ|PD;hQi=>
High5 NVfIfld{U2mwYYPlJGF{e2G7 NEfHbGczPCCq NXLlVIRHUW6qaXLpeIlwdiCxZjDoeY1idiC{ZXPvcYJqdmGwdDDISGFEOyC5aYToJGlEPTBib3[gNE4yPyEQvF2= NIG4N4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{M{[wNlUzOyd-MkO2NFI2OjN:L3G+
A549 NIDnVJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Ml;sSG1UVw>? NGLXS|RKSzVyPUGuNFc6KM7:TR?= M4j2[|xiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|NkS0NlExLz5{M{[0OFIyODxxYU6=
HCT116 NFTQT5hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnPPSG1UVw>? MknMTWM2OD1yLke1O{DPxE1? MoTPQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN4NESyNVAoRjJ|NkS0NlExRC:jPh?=
K562 Ml;3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLJSG1UVw>? NH3RPIhKSzVyPUCuOlQ2KM7:TR?= NXLEVmRyRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkO2OFQzOTBpPkKzOlQ1OjFyPD;hQi=>
GBC-SD MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHnXZYs2KM7:TR?= MmntO|IhcA>? Mny0SG1UVw>? M2q0VmlEPTB;MUSuPEDPxE1? M3jYVVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ|N{C3NlYxLz5{M{ewO|I3ODxxYU6=
HGC27 NELZZVdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrUSXI2KM7:TR?= M4TWXFczKGh? M3TyVWROW09? NFT3UGRKSzVyPUWuOEDPxE1? MXi8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zOzdyN{K2NEc,OjN5MEeyOlA9N2F-
HL60 MVLLbY5ie2ViQYPzZZk> M1HlPFUh|ryP MnjWNlQhcA>? M3HaSGROW09? MVrJcohq[mm2aX;uJI9nKEiGQVO= MofWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5MEeyOlAoRjJ|N{C3NlYxRC:jPh?=
Vero MkTaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkfBO|IhcA>? NXn6TJRtTE2VTx?= NITBPGpKSzVyPUCuPUDPxE1? MkLSQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjN5OE[0OVIoRjJ|N{i2OFUzRC:jPh?=
NCI60 M{PuPGN6fG:2b4jpZ{BCe3OjeR?= MkDoOFghcA>? M{PMWmROW09? NFvqWXZIUT1yLkWzJO69VQ>? MoXYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUWzNlcoRjJ2MEG1N|I4RC:jPh?=
SW620 M1fEVmN6fG:2b4jpZ{BCe3OjeR?= M1;xZVQ5KGh? M3rYdGROW09? NFPkXJJIUT1yLkW0JO69VQ>? MoLWQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUWzNlcoRjJ2MEG1N|I4RC:jPh?=
U251 M2LTZ2N6fG:2b4jpZ{BCe3OjeR?= M1e0S|Q5KGh? MmnMSG1UVw>? M33xcGdKRTFwNUOg{txO MVS8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDBzNUOyO{c,OjRyMUWzNlc9N2F-
KM12 Mn;YR5l1d3SxeHnjJGF{e2G7 M4\STFQ5KGh? NYTlOZBjTE2VTx?= M{S1S2dKRTFwOEig{txO MkexQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUWzNlcoRjJ2MEG1N|I4RC:jPh?=
Hs578T MYXDfZRwfG:6aXOgRZN{[Xl? NU\sfYtGPDhiaB?= NVuyV2VoTE2VTx?= M1XM[GdKRTRwOEOg{txO MnPkQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRyMUWzNlcoRjJ2MEG1N|I4RC:jPh?=
MDA-MB-231 NIjXVpRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfyOFghcA>? NIPWXplKSzVyPUCuNVYh|ryP NFLHfmg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEC5OVAyPid-MkSwPVUxOTZ:L3G+
MCF7 MnPiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXO0PEBp MYDHTV0xNjR3IN88US=> MXu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDFzOUW1OUc,OjRzMUm1OVU9N2F-
MDA-MB-435 NWT1b2dFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXLaS5RyPDhiaB?= NUnjRoZ[T0l;MD64PEDPxE1? MkWwQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjRzMUm1OVUoRjJ2MUG5OVU2RC:jPh?=
AsPC1 NYTmZ|BET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUezNEDPxE1? NHPEZmc1QCCq M2XtdmROW09? M2LaUGlEPTB;Mz62OkDPxE1? MVu8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDF6NUO3PEc,OjRzOEWzO|g9N2F-
Jurkat E6.1 NVnmN|EzU2mwYYPlJGF{e2G7 NIPBbmkzKGh? NYj2UoJzTE2VTx?= NH3XTohKdmirYnn0bY9vKG:oIFjERWM1KHerdHigTWM2OCCxZjCwMlA1KM7:TR?= NWHGZXlkRGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkSyOlE5PjJpPkK0NlYyQDZ{PD;hQi=>
KG1 NH3OcHNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mln5OFghcA>? MlHoTWM2OD1zLkG1JO69VQ>? MWm8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDV{NUCwN{c,OjR3MkWwNFM9N2F-
HEL MmL2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV60PEBp MUTEUXNQ MnX3TWM2OD1yLkS5JO69VQ>? Ml70QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR4OUSwOVUoRjJ2Nkm0NFU2RC:jPh?=
HSC3 MnrkR5l1d3SxeHnjJGF{e2G7 NYjQNIR5OTJiaB?= NYe2bHJSTE2VTx?= NUiyS|QyUUN3ME2wMlcyKM7:TR?= MmrYQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5Nk[1OlAoRjJ2N{[2OVYxRC:jPh?=
HT-29 MnL0R5l1d3SxeHnjJGF{e2G7 NFK0UZoyOiCq M2r1eGROW09? NEPONHZKSzVyPUCuO|Ih|ryP M1nybVxiKHSjcnfleF0oZ2KuYX7rK{BpemWoPTfoeJRxezpxL4D1Zo1m\C6wY3LpMo5tdS6waXiu[493NzJ2N{[2OVYxLz5{NEe2OlU3ODxxYU6=
KB MnHzR5l1d3SxeHnjJGF{e2G7 NEXJbHUyOiCq NFy4[ItFVVOR M4fVOWlEPTB;MD63OUDPxE1? MkPvQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5Nk[1OlAoRjJ2N{[2OVYxRC:jPh?=
H460 NYX6b4dJS3m2b4TvfIlkKEG|c3H5 MoLQNVIhcA>? M1W1emROW09? MlfKTWM2OD1yLkiyJO69VQ>? NFnERXA9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2OlU3OCd-MkS3OlY2PjB:L3G+
MONE1 MV7DfZRwfG:6aXOgRZN{[Xl? M4m4WlEzKGh? NX7we4M{TE2VTx?= NXnhdINQUUN3ME2xMlE5KM7:TR?= NHO3[ZY9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEe2OlU3OCd-MkS3OlY2PjB:L3G+
MKN45 MlH6R5l1d3SxeHnjJGF{e2G7 NV3Zc|NyOTJiaB?= MVHEUXNQ MWnJR|UxRTFwMkWg{txO MU[8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zPDd4NkW2NEc,OjR5Nk[1OlA9N2F-
TSGH NXi2PJlrS3m2b4TvfIlkKEG|c3H5 MV:xNkBp MWfEUXNQ M4fGb2lEPTB;Mj6wPUDPxE1? MnX1QIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR5Nk[1OlAoRjJ2N{[2OVYxRC:jPh?=
HL60 M{jETmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmHWO|IhcA>? MV7EUXNQ NITOO4xIUTVyPUCuPVMh|ryP NIO0T4s9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{NEmwNFUxOCd-MkS5NFA2ODB:L3G+
HEK293 MonDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDuW4w{PzJiaB?= NESxfZFFVVOR MYTHTVUxRTF|IN88US=> MoXmQIEhfGG{Z3X0QUdg[myjbnunJIhz\WZ;J3j0eJB{Qi9xcIXicYVlNm6lYnmucoxuNm6raD7nc5YwOjR7MEC1NFAoRjJ2OUCwOVAxRC:jPh?=
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Assay
Methods Test Index PMID
Western blot Ac-Histone H4 / Ac-Histone H3 ; phospho-CDK1 ; phospho-Cdc25c / Cdc25c ; Cyclin B1 ; c-Myc / c-Raf / Akt / Cyclin D1 / CDK4 ; p-eIF2α / ATF4 / CHOP ; p21 21598070 19440035 20072656
Immunofluorescence HDAC1 / CK19 ; Nrf2 ; α-SMA ; Ac-STAT3 / IGF2 29917299 20566897 26330748 27086926
Growth inhibition assay Cell apoptosis ; Cell viability 19440035 27086926
ELISA IGF2 ; IL-13 / IL-10 / IL-5 ; M-CSF ; MMP-9 27086926 18541724 23042302
In vivo Administration of Vorinostat (~100 mg/kg/day) significantly inhibits the growth of CWR22 human prostate xenografts in nude mice with tumor reductions of 78%, 97% and 97%, at doses of 25 mg/kg/day, 50 mg/kg/day and 100 mg/kg/day, respectively, compared with control. Vorinostat induces the accumulation of acetylated core histones and prostate-specific antigen mRNA expression in CWR22 cells, resulting in higher levels of serum prostate-specific antigen than predicted from tumor volume alone. [2] Oral administration of Vorinostat (0.67g/L) crosses the blood-brain barrier, increases histone acetylation in the brain, and dramatically improves the motor impairment in the R6/2 mice model of Huntington's disease. [5]

Protocol (from reference)

Kinase Assay:

[1]

  • Immunoprecipitation-HDAC assays:

    The lysate of Jurkat cells is incubated for 1 hour on ice and cleared by centrifugation at 12,000 g for 10 minutes at 4 °C. Supernatants are precleared with 30 μL of 50% protein G-Sepharose slurry for 1 hour at 4 °C. Beads are pelleted by centrifugation and supernatants are incubated for 1 hour at 4 °C with 10 μg of IgG fraction from anti-HDAC1 or HDAC3 polyclonal antisera (preincubated 2 hours at room temperature with either the homologous or heterologous immunizing peptide). Both antisera are raised in rabbits against the carboxylterminal peptide of HDAC1 and HDAC3 by using synthetic peptides coupled to keyhole limpet hemocyanin. 30 μL of 50% protein G-Sepharose slurry is added for 1 hour at 4 °C. Immune complexes are pelleted by centrifugation and washed three times with 1 mL of lysis buffer. Beads are resuspended in 200 μL of HDAC buffer (20 mM Tris-HCl, pH 8.0/150 mM NaCl/10% glycerol), and the HDAC assay is performed with an 3H-acetylated peptide corresponding to amino acids 1-24 of histone H4. Released [3H]acetic acid is quantified by scintillation counting. For inhibitions studies, the immunoprecipitated complexes are preincubated with the different concentrations of Vorinostat for 30 minutes at 4 °C.

Cell Research:

[2]

  • Cell lines: LNCaP, PC-3, and TSU-Pr1
  • Concentrations: Dissolved in DMSO, final concentrations ~7.5 μM
  • Incubation Time: 1, 2, 3 and 4 days
  • Method:

    Cells are exposed to various concentrations of Vorinostat for 1, 2, 3 and 4 days. Cell viability is assessed by trypan blue dye exclusion.

Animal Research:

[2]

  • Animal Models: Male BALB/c nude (nu/nu) mice implanted with CWR22 tumor cells
  • Dosages: ~100 mg/kg/day
  • Administration: Injection i.p.

Solubility (25°C)

In vitro

DMSO 52 mg/mL
(196.74 mM)
Water Insoluble
Ethanol '''''3 mg/mL

In vivo

Add solvents to the product individually and in order
(Data is from Selleck tests instead of citations):
2% DMSO+30% PEG 300+5% Tween 80+ddH2O
For best results, use promptly after mixing.

5mg/mL

Chemical Information

Molecular Weight 264.3
Formula

C14H20N2O3

CAS No. 149647-78-9
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC=C(C=C1)NC(=O)CCCCCCC(=O)NO

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03022565 Withdrawn Drug: Vorinostat Uveal Melanoma University of Miami|University of Miami Sylvester Comprehensive Cancer Center January 2020 Early Phase 1
NCT03167437 Recruiting Drug: Vorinostat|Drug: Ustekinumab Crohn''s Disease National Institute of Allergy and Infectious Diseases (NIAID)|National Institutes of Health Clinical Center (CC) October 30 2017 Phase 1|Phase 2
NCT03056495 Recruiting Drug: N-hydroxy-N''-phenyl-octanediamide (Vorinostat) Alzheimer Disease German Center for Neurodegenerative Diseases (DZNE)|University Hospital Bonn|University of Göttingen September 28 2017 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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Frequently Asked Questions

Question 1:
What’s the recommendation about reconstitution of the compound for in vivo animal study?

Answer:
We recommend the following vehicle for S1047: 2% DMSO, 40% PEG 300, 5% Propylene glycol, 1% Tween 80 at 5 mg/mL.

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