Cell-free Assays (IC50/Ki) for Vorinostat (SAHA)

Key TargetIC50 (Cell-free)DescriptionReference
HDAC1 0.08 µg/mL color de lys ELISA assay kits Download PDF
HDAC6 0.09 µg/mL color de lys ELISA assay kits Download PDF
HDACs (from SiHa cell nuclear extracts) 0.18 µM Fluorescent-based HDAC assay (using Boc-Lys(Ac)-AMC substrate) Download PDF
Total HDAC (from K562 nuclear extracts), HDAC1, HDAC2, HDAC3, HDAC6, HDAC8, HDAC10, HDAC11 23 nM (Total HDAC), 89 nM (HDAC1), 91 nM (HDAC2), 77 nM (HDA Fluorescence signal measurement using SpectraMax i3x fluorimeter with SoftMax Pro software, followed by nonlinear regression using Prism 9.0 software. Download PDF
HDAC1 0.009 μM Fluorogenic peptide assay using p53 residues 379–382 (RHKK(Ac)AMC) substrate, tested in a 10-dose IC50 with 3-fold serial dilution starting at 10 μM. Download PDF

Cell-based Assay (IC50) for Vorinostat (SAHA)

Cell TypeIC50 (Cell-based)DescriptionReference
PC3 > 1.8 μM Cell cytotoxicity assays were performed in 96-well plates to determine the growth kinetics of treated cells using CCK-8 reagent. Download PDF
DU145 > 1.8 μM Cell cytotoxicity assays were performed in 96-well plates to determine the growth kinetics of treated cells using CCK-8 reagent. Download PDF
MDA-MB-231 3.5 µM 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; Calculation of IC50 was obtained by fitting the cytotoxicity responses to a variable slope sigmoidal equation (log inhibitor vs. normalized response). Download PDF
MCF-7 4.2 µM 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; Calculation of IC50 was obtained by fitting the cytotoxicity responses to a variable slope sigmoidal equation (log inhibitor vs. normalized response). Download PDF
MCF-10A 3.0 µM 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay; Calculation of IC50 was obtained by fitting the cytotoxicity responses to a variable slope sigmoidal equation (log inhibitor vs. normalized response). Download PDF
Check the Vorinostat (SAHA) product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

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