Ribavirin (ICN-1229)

For research use only.

Catalog No.S2504 Synonyms: NSC-163039, RTCA, Tribavirin

16 publications

Ribavirin (ICN-1229) Chemical Structure

CAS No. 36791-04-5

Ribavirin (NSC-163039, ICN-1229, RTCA, Tribavirin), a synthetic guanosine analogue, possesses a broad spectrum of activity against DNA and RNA viruses.

Selleck's Ribavirin (ICN-1229) has been cited by 16 publications

3 Customer Reviews

  • (E) Left panel, Western blot showed that 20 μM Ribavirin effectively inhibits elevated Sox2 expression in irradiated SW1990 cells. Right panel, qPCR analysis showed that Sox2 mRNA level in irradiated SW1990 cells at 48 hours was lower than that in non-irradiated SW1990 cells, *p < 0.05. (F) Left panel, Western blot showed 20 μM Ribavirin inhibits elevated Sox2 expression in irradiated BxPc-3 cells. Right panel, qPCR analysis showed that there is no significant fluctuation of Sox2 mRNA in irradiated BxPc-3 cells and Ribavirin did not influence sox2 mRNA level in irradiated BxPc-3 cells.

    Cancer Lett, 2016, 375(1):31-8. . Ribavirin (ICN-1229) purchased from Selleck.

  • Inhibition of viral replication and ensuing myocardial apoptosis ameliorates HDACI-exacerbated viral myocarditis. (A) CVB3-infected cardiac myocytes (MOI, 5) were treated with 1 μM SAHA and the indicated concentrations of ribavirin for 24 h. Cell extracts were analyzed by Western blotting with the indicated antibodies. (B) CVB3-infected cardiac myocytes (MOI, 5) were treated with 1 μM SAHA and 200 μg/ml ribavirin for 24 h. CVB3 titers in cell culture supernatants were determined by TCID50 assay (n = 4). (C) Cardiac myocytes were treated with 200 μg/ml ribavirin for 24 h. Cells morphology was examined by light microscope (scale bar, 100 μm). (D to F) CVB3-infected cardiac myocytes (MOI, 5) were treated with 2 μM SAHA in the presence or absence of 800 μg/ml ribavirin for 24 h. (D) Cell morphology was examined by light microscope (scale bar, 100 μm). (E and F) Cells were collected and stained with 7-AAD and FITC-labeled annexin V, followed by flow cytometric analysis (n = 3). (G and H) BALB/c mice were infected with CVB3 on day 0 and then simultaneously treated with SAHA (50 mg/kg) and ribavirin (100 mg/kg) or vehicle PBS daily from day 0 to day 7 p.i. (G) Survival rate was monitored daily until day 7 p.i. (n = 10). (H) Paraffin sections of heart tissues were prepared on day 7 p.i., and cardiac injury was revealed by H&E (scale bar, 50 μm). *, P < 0.05; ***, P < 0.001.

    J Virol, 2015, 89(20):10512-23.. Ribavirin (ICN-1229) purchased from Selleck.

  • Establishment of Vero-rBoDV-1-Gluc cells to monitor BoDV-1 replication. (A) Dose- and time-dependent inhibitory effects of ribavirin on BoDV-1 replication. Vero-rBoDV-1-Gluc cells were treated with 20 or 40 μM of ribavirin for the indicated periods, and the luciferase activity in the cell supernatant was measured. (B) Quantification of BoDV-1 RNAs in Vero-rBoDV-1-Gluc cells treated with 20 or 40 μM of ribavirin for 72 h. Values are expressed as the mean + S.E. from 3 biological replicates. *, p < 0.05; **, p < 0.01; ****, p < 0.001.

    Antiviral Res, 2017, 143:237-245. Ribavirin (ICN-1229) purchased from Selleck.

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Biological Activity

Description Ribavirin (NSC-163039, ICN-1229, RTCA, Tribavirin), a synthetic guanosine analogue, possesses a broad spectrum of activity against DNA and RNA viruses.
In vitro

Ribavirin significantly reduces the efficiency with which progeny subgenomic replicons transfect new cells in the replicon system, although ribavirin has little effect on levels of HCV replication. Ribavirin increases the mutation frequency of HCV, with the highest rates of mutations being found in the NS5A-encoding region. Ribavirin enhances TH1 while inhibiting T 2 cytokine production by stimulated T cells. [1] Ribavirin shows antiviral activity against a variety of RNA viruses and is used in combination with interferon-alpha to treat hepatitis C virus infection. Ribavirin reduces infectious poliovirus production to as little as 0. 00001% in cell culture. [2] Ribavirin's antiviral activity is exerted directly through lethal mutagenesis of the viral genetic material. [3] Ribavirin markedly reduces viral-induced parameters of macrophage activation at physiologic concentrations (up to 500 mg/mL). Ribavirin inhibits the production of IL-4 by Th2 cells, whereas it does not diminish the production of IFN-gamma in Th1 cells. [4] Ribavirin exhibits antiviral activity against a broad range of both DNA and RNA viruses in vitro. Ribavirin is a cytostatic agent and causes a reduction in synthesis of DNA, RNA and proteins in exposed cells. Ribavirin is thought to induce a switch in T-helper cell phenotype from type 2 to type 1. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
E6SM cell lines MnnFSpVv[3Srb36gZZN{[Xl? MWPF[oZm[3SrdnWgZ49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcohq[mm2IFjldpBmeyC|aX3wcIV5KH[rcoXzMVEpUFOYLUGpJE8hfGi7bXnu[UBscW6jc3Wg[IVncWOrZX70JEhVUy1rQkKwNFYhNyCYTWexPFM4KGmwZIXj[YQh[3m2b4DheIhq[2m2eTDifUA2OCViaX6gSVZUVSClZXzsJIxqdmW|LDDFR|UxRTBwMEC1JO69VQ>? NXz1RVhSOTF2OUW1PFY>
MDCK cells M4XhbWZ2dmO2aX;uJIF{e2G7 Mnz4N{Bl[Xm| NIfmNHVCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBKdm[udXXufoEhSiC4aYL1d{ApSi:KSz:1M|czMSCrbn\lZ5Rm\CCrbjDNSGNMKGOnbHzzJIF{e2W|c3XkJIF{KH[rcoXzMYlv\HWlZXSgZ5l1d3CjdHjpZ{Bm\m[nY4SgZYZ1\XJiMzDkZZl{KGK7IH3pZ5Jwe2OxcHnjJIFv[Wy7c3nzMEBGSzVyPUCuNFk1KM7:TR?= MWWyN|EyPzF5Mx?=
mouse L1210 cells NE\wcZJHfW6ldHnvckBie3OjeR?= MUKyNE03OCCvaX7z NIHTS2tKdmirYnn0bY9vKG:oIFnNVGRJKGmwIH3veZNmKExzMkGwJINmdGy|IHHzd4V{e2WmIHHzJIZwem2jdHnvckBw\iCdMjy4MVNJZWi7cH;4ZY51cGmwZTDmdo9uKFt{LEitN2heUU2SIHHmeIVzKDJyIITvJFYxKG2rboOsJGlEPTB;MTFOwG0> NFG2RmYzOjV3NUG1Ni=>
human CEM cells NY\wRVRXTnWwY4Tpc44h[XO|YYm= NGT6S5MzOC14MDDtbY5{ NFjsNo9KdmirYnn0bY9vKG:oIFnNVGRJKGmwIHj1cYFvKEOHTTDj[YxteyCjc4Pld5Nm\CCjczDmc5Ju[XSrb36gc4YhYzJuOD2zTH1pgXCxeHHueIhqdmViZoLvcUBcOix6LUPIYWlOWCCjZoTldkAzOCC2bzC2NEBucW6|LDDJR|UxRTFizszN MnKwNlI2PTVzNUK=
BHK21 cell line MmXuSpVv[3Srb36gZZN{[Xl? NYfGVWpzUW6qaXLpeIlwdiCxZjDX[ZN1KE6rbHWgeolzfXNiVlzQJJJmeGyrY3;uJIlvKEKKS{KxJINmdGxibHnu[UwhTUN3ME2xMlEh|ryP NHW4[nMyPjV|OUSwNi=>
HeLa cell NV3tOIl{TnWwY4Tpc44h[XO|YYm= MVrF[oZm[3SrdnWgZ49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcohq[mm2IILld5BqemG2b4L5JJN6dmGydHnhcEhTW1ZrII\pdpV{NWmwZIXj[YQh[3m2b4DheIhq[2m2eTDifUA2OCViaX6gTIVN[SClZXzsJIxqdmW|LDDFR|UxRTFwNTFOwG0> Mn\mNVE1QTV3OE[=
MDCK cells NVe0Z5dyTnWwY4Tpc44h[XO|YYm= NWjUXlJ[OzZiaB?= MojsRY51cWmwZnz1[Y57[SCjY4Tpeol1gSCjZ3HpcpN1KGmwZnz1[Y57[SCDII\pdpV{KEhzTkGgbY5n\WO2ZXSgbY4hVUSFSzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcoXzMYlv\HWlZXSgZ5l1d3CjdHjpZ{Bm\m[nY4SgZYZ1\XJiM{[gbJJ{NCCLQ{WwQVEvQSEQvF2= MX2yNlM1OTl2Mx?=
MDBK cells NWnLVG16TnWwY4Tpc44h[XO|YYm= M3fBUlIh\GG7cx?= M2n4d2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGJwfmmwZTD2bZJidCCmaXHydohm[SC4aYL1d{AyNU6DRFygRXRESyCYUkWzOEBqdm[nY4Tl[EBqdiCPRFLLJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBw\iC4aYLhcEBkgXSxcHH0bIlkKGWoZnXjeEBi\nSncjCyJIRigXNiYoSgUXRUKGG|c3H5MEBGSzVyPUSuOkDPxE1? MnizNlI6PDR|M{O=
african green monkey Vero cells MojpSpVv[3Srb36gZZN{[Xl? NIPZUoY2KGSjeYO= NXTZVnBxSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhWlOYIFGyJIlv\mWldHXkJIlvKGGocnnjZY4h\3KnZX6gcY9vc2W7IG\ldo8h[2WubIOgZYZ1\XJiNTDkZZl{KGK7IIDsZZF2\SC{ZXT1Z5Rqd25iYYPzZZktKEWFNUC9O{DPxE1? MWGyNFM2QTh7OB?=
HEL cells MV;GeY5kfGmxbjDhd5NigQ>? MWKyMVMh\GG7cx?= NFrBcZhCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBJ\XKyZYOgd4lueGyneDD2bZJ2eyBzIFvPV{Bqdm[nY4Tl[EBqdiCKRVygZ4VtdHNiYYPz[ZN{\WRiYYOgdJJwfGWldHnvckBnem:vII\pdpV{NWmwZIXj[YQh[3m2b4DheIhw\2WwaXPpeJkh[W[2ZYKgNkB1dyB|IHThfZMh[nliTWTUJIF{e2G7LDDFR|UxRTFyIN88US=> NVHtSHd4OjByM{S3NVE>
HEK293 cells M33zWmZ2dmO2aX;uJIF{e2G7 MUGyOEBp NELzXIFKdmirYnn0bY9vKG:oIHnu[ox2\W68YTDBJJZqenW|IGLORU1l\XCnbnTlcpQhWk6DIIDvcJlu\XKjc3Wg[ZhxemW|c3XkJIlvKEiHS{K5N{Bk\WyuczDh[pRmeiB{NDDodpMh[nliZIXhcEBtfWOrZnXyZZNmKHKncH;yeIVzKGenbnWgZZN{[XluIFXDOVA:OTVizszN MUSyOFMyOzd|MB?=
human BE(2)-C cells M1[4[mZ2dmO2aX;uJIF{e2G7 MWmxPE0zOCCq NWjMVItwSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhX2W|dHXyckBmeXWrbnWg[Y5k\XCqYXzvcZlmdGm2aYOgeolzfXNiaX7m[YN1\WRiaX6gbJVu[W5iQlWoNkkuSyClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqemGuIGLORUBz\XCuaXPheIlwdiCjZoTldkAyQCC2bzCyNEBpenNiYomgcJVkcW[ncnHz[UBz\XCxcoTldkBo\W6nIHHzd4F6NCCLQ{WwQVE3KM7:TR?= MnS0NlQyPTF7NUS=
human HuH7-J20 cells MVXGeY5kfGmxbjDhd5NigQ>? MkTWRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTGNXKEqISEGgbY5n\WO2ZXSgbY4hcHWvYX6gTJVJPy2MMkCgZ4VtdHNiYYPz[ZN{\WRiYYOgbY5pcWKrdHnvckBw\iC4aYLhcEBtcW[nIHP5Z4xmKGK7IH3lZZN2emmwZzDz[YNz\XSnZDDhcItidGmwZTDwbI9{eGijdHHz[UBt\X[nbDDwdoVqdmO3YnH0[YQhf2m2aDDj[YxteyCob4KgNUBpeiCob3zsc5dm\CCkeTD2bZJidCCrbn;jeYxifGmxbjDmc5IhOyCqcoOgcYVie3W{ZXSgZZQhPzJiaILzMEBGSzVyPUOwMlQh|ryP Mn;zNlY1Ojh6N{C=
human MT4 cells NG\vTY5EgXSxdH;4bYPDqGG|c3H5 M4jid|k3KGh? NIHWTmtEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOXDRiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JG1VXCCjc4PhfUwhS0N3ME2zNUDPxE1? NGH5XnEyQTR6MkS4NS=>
human HuH7 cells Mn61R5l1d3SxeHnjxsBie3OjeR?= NHPDRmQ{KGSjeYO= NHuwephEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBJfUh5IHPlcIx{KGmwZnXjeIVlKHerdHigTGNXOWJiYX\0[ZIhOyCmYYnzJIJ6KE2WUzDhd5NigSxiQ1O1NF0{OiEQvF2= MmLYNlIyODB{NU[=
human KB cells M{T6NmZ2dmO2aX;uJIF{e2G7 M1[3cVczKGh? M{X3cGFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHJpcW6xdnnyeZMhfHmyZTCxN{Bqdm[nY4Tl[EBqdiCqdX3hckBMSiClZXzsd{Bie3Onc4Pl[EBieyCrbnjpZol1cW:wIH;mJJZqenW|LXnu[JVk\WRiY4n0c5BifGirYzDl[oZm[3RiYX\0[ZIhPzJiaILzJJJmdGG2aY\lJJRwKGOxboTyc4w> M4PIPVczOjd|MB?=
human KB cells M3vRdmZ2dmO2aX;uJIF{e2G7 NVTGSoxbOS1zMECwJO69\y:vbB?= NWHGeJJUPzJiaB?= NEjTcYFCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBTcGmwb4\pdpV{KHS7cHWgNVMhcW6oZXP0[YQhcW5iaIXtZY4hU0JiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXK3cz3pcoR2[2WmIHP5eI9x[XSqaXOg[YZn\WO2IHH0JFEhfG9iMUCwNEB2\y:vbDDh[pRmeiB5MjDodpM> MmTJNlEyOjN2

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p53 / p-p53 / p21 / Mdm2 ; 

PubMed: 22962590     


Dose-dependent expression and phosphorylation of p53 induced by ribavirin. HepG2 cells were treated with the indicated doses of ribavirin (0, 5, 10, 30, 100 µg/ml) for 24 h.

EZH2 / p-ERK / ERK / p-eIF4E / eIF4E / p21 ; 

PubMed: 29487714     


Western blot analyses of EZH2, ERK1/2, eIF4E, and p21 expression in BT12, BT16, and BT37 cells 72 and 96hrs after ribavirin treatment. 

Cyclin D1 ; 

PubMed: 21415224     


Cyclin D1, NBS1 and VEGF protein levels were assessed by western blot in MDA-MB-468 and BT474 cells, treated with the indicated concentrations of ribavirin for 3 days. 

p-AKT / AKT / pBP1 / BP1 ; 

PubMed: 21415224     


AKT activity was assessed as the level of phosphorylation of AKT and its downstream target 4E-BP1 in MDA-MB-468 and BT474 cells treated with the indicated concentrations of ribavirin for 3 days. 

22962590 29487714 21415224
Growth inhibition assay
Cell number ; 

PubMed: 21415224     


Breast cancer cell lines were cultured in the absence or presence of ribavirin at the indicated concentrations for 7 days, and the number of cells was determined.

21415224

Protocol

Solubility (25°C)

In vitro DMSO 49 mg/mL (200.64 mM)
Water 49 mg/mL (200.64 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 244.20864
Formula

C8H12N4O5

CAS No. 36791-04-5
Storage powder
in solvent
Synonyms NSC-163039, RTCA, Tribavirin
Smiles C1=NC(=NN1C2C(C(C(O2)CO)O)O)C(=O)N

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04283513 Not yet recruiting Drug: Virazole Hemorrhagic Fever U.S. Army Medical Research and Development Command June 2021 Phase 2
NCT04335123 Completed Drug: Losartan COVID-19 University of Kansas Medical Center April 4 2020 Phase 1
NCT04285034 Recruiting -- Lassa Fever University of Oxford November 26 2019 --
NCT03889106 Unknown status Drug: Ribavirin Lassa Fever University of Oxford|National Institute for Health Research United Kingdom|Kenema Government Hospital|London School of Hygiene and Tropical Medicine|Public Health England March 1 2019 --
NCT03585725 Recruiting Drug: Ribavirin Follicular Lymphoma|Mantle Cell Lymphoma Weill Medical College of Cornell University|Memorial Sloan Kettering Cancer Center September 26 2018 Early Phase 1

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID