Ribavirin

Catalog No.S2504 Synonyms: NSC-163039, ICN-1229 ,RTCA, Tribavirin

Ribavirin  Chemical Structure

Molecular Weight(MW): 244.20864

Ribavirin, a synthetic guanosine analogue, possesses a broad spectrum of activity against DNA and RNA viruses.

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In DMSO USD 90 In stock
USD 70 In stock
USD 120 In stock
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Cited by 6 Publications

3 Customer Reviews

  • (E) Left panel, Western blot showed that 20 μM Ribavirin effectively inhibits elevated Sox2 expression in irradiated SW1990 cells. Right panel, qPCR analysis showed that Sox2 mRNA level in irradiated SW1990 cells at 48 hours was lower than that in non-irradiated SW1990 cells, *p < 0.05. (F) Left panel, Western blot showed 20 μM Ribavirin inhibits elevated Sox2 expression in irradiated BxPc-3 cells. Right panel, qPCR analysis showed that there is no significant fluctuation of Sox2 mRNA in irradiated BxPc-3 cells and Ribavirin did not influence sox2 mRNA level in irradiated BxPc-3 cells.

    Cancer Lett, 2016, 375(1):31-8. . Ribavirin purchased from Selleck.

    Inhibition of viral replication and ensuing myocardial apoptosis ameliorates HDACI-exacerbated viral myocarditis. (A) CVB3-infected cardiac myocytes (MOI, 5) were treated with 1 μM SAHA and the indicated concentrations of ribavirin for 24 h. Cell extracts were analyzed by Western blotting with the indicated antibodies. (B) CVB3-infected cardiac myocytes (MOI, 5) were treated with 1 μM SAHA and 200 μg/ml ribavirin for 24 h. CVB3 titers in cell culture supernatants were determined by TCID50 assay (n = 4). (C) Cardiac myocytes were treated with 200 μg/ml ribavirin for 24 h. Cells morphology was examined by light microscope (scale bar, 100 μm). (D to F) CVB3-infected cardiac myocytes (MOI, 5) were treated with 2 μM SAHA in the presence or absence of 800 μg/ml ribavirin for 24 h. (D) Cell morphology was examined by light microscope (scale bar, 100 μm). (E and F) Cells were collected and stained with 7-AAD and FITC-labeled annexin V, followed by flow cytometric analysis (n = 3). (G and H) BALB/c mice were infected with CVB3 on day 0 and then simultaneously treated with SAHA (50 mg/kg) and ribavirin (100 mg/kg) or vehicle PBS daily from day 0 to day 7 p.i. (G) Survival rate was monitored daily until day 7 p.i. (n = 10). (H) Paraffin sections of heart tissues were prepared on day 7 p.i., and cardiac injury was revealed by H&E (scale bar, 50 μm). *, P < 0.05; ***, P < 0.001.

    J Virol, 2015, 89(20):10512-23.. Ribavirin purchased from Selleck.

  • Establishment of Vero-rBoDV-1-Gluc cells to monitor BoDV-1 replication. (A) Dose- and time-dependent inhibitory effects of ribavirin on BoDV-1 replication. Vero-rBoDV-1-Gluc cells were treated with 20 or 40 μM of ribavirin for the indicated periods, and the luciferase activity in the cell supernatant was measured. (B) Quantification of BoDV-1 RNAs in Vero-rBoDV-1-Gluc cells treated with 20 or 40 μM of ribavirin for 72 h. Values are expressed as the mean + S.E. from 3 biological replicates. *, p < 0.05; **, p < 0.01; ****, p < 0.001.

    Antiviral Res, 2017, 143:237-245. Ribavirin purchased from Selleck.

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Biological Activity

Description Ribavirin, a synthetic guanosine analogue, possesses a broad spectrum of activity against DNA and RNA viruses.
In vitro

Ribavirin significantly reduces the efficiency with which progeny subgenomic replicons transfect new cells in the replicon system, although ribavirin has little effect on levels of HCV replication. Ribavirin increases the mutation frequency of HCV, with the highest rates of mutations being found in the NS5A-encoding region. Ribavirin enhances TH1 while inhibiting T 2 cytokine production by stimulated T cells. [1] Ribavirin shows antiviral activity against a variety of RNA viruses and is used in combination with interferon-alpha to treat hepatitis C virus infection. Ribavirin reduces infectious poliovirus production to as little as 0. 00001% in cell culture. [2] Ribavirin's antiviral activity is exerted directly through lethal mutagenesis of the viral genetic material. [3] Ribavirin markedly reduces viral-induced parameters of macrophage activation at physiologic concentrations (up to 500 mg/mL). Ribavirin inhibits the production of IL-4 by Th2 cells, whereas it does not diminish the production of IFN-gamma in Th1 cells. [4] Ribavirin exhibits antiviral activity against a broad range of both DNA and RNA viruses in vitro. Ribavirin is a cytostatic agent and causes a reduction in synthesis of DNA, RNA and proteins in exposed cells. Ribavirin is thought to induce a switch in T-helper cell phenotype from type 2 to type 1. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
E6SM cell lines MkPhSpVv[3Srb36gZZN{[Xl? NIjrV4NG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JGhmenCnczDzbY1xdGW6II\pdpV{NTFqSGPWMVEqKC9idHj5cYlv\SCtaX7hd4Uh\GWoaXPp[Y51KCiWSz2pRlIxODZiLzDWUXcyQDN5IHnu[JVk\WRiY4n0c5BifGirY3n0fUBjgSB3MDWgbY4hTT[VTTDj[YxtKGyrbnXzMEBGSzVyPUCuNFA2KM7:TR?= NV3ib49vOTF2OUW1PFY>
MDCK cells MlS0SpVv[3Srb36gZZN{[Xl? NWX1Zo9EOyCmYYnz NXzEPJl5SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUW6obIXlcppiKEJidnnyeZMhMEJxSFuvOU84OiliaX7m[YN1\WRiaX6gUWREUyClZXzsd{Bie3Onc4Pl[EBieyC4aYL1d{1qdmS3Y3XkJIN6fG:yYYTobYMh\W[oZXP0JIFnfGW{IEOg[IF6eyCkeTDtbYNzd3Olb4DpZ{BidmGueYPpd{whTUN3ME2wMlA6PCEQvF2= MYGyN|EyPzF5Mx?=
mouse L1210 cells NXj5TIhUTnWwY4Tpc44h[XO|YYm= NVuzT3BjOjBvNkCgcYlvew>? NWLSTIw3UW6qaXLpeIlwdiCxZjDJUXBFUCCrbjDtc5V{\SCOMUKxNEBk\WyuczDhd5Nme3OnZDDhd{Bnd3KvYYTpc44hd2ZiW{KsPE0{UF2qeYDvfIFvfGirbnWg[pJwdSCdMjy4MVNJZUmPUDDh[pRmeiB{MDD0c{A3OCCvaX7zMEBKSzVyPUGg{txO M3\O[lIzPTV3MUWy
human CEM cells NWP1cYVITnWwY4Tpc44h[XO|YYm= NIPYOngzOC14MDDtbY5{ M3jyOGlvcGmkaYTpc44hd2ZiSV3QSGghcW5iaIXtZY4hS0WPIHPlcIx{KGG|c3Xzd4VlKGG|IH\vdo1ifGmxbjDv[kBcOix6LUPIYYh6eG:6YX70bIlv\SCocn;tJHszNDhvM1jdTW1RKGGodHXyJFIxKHSxIE[wJI1qdnNuIFnDOVA:OSEQvF2= Mle1NlI2PTVzNUK=
BHK21 cell line M4ruO2Z2dmO2aX;uJIF{e2G7 NXzxUFV6UW6qaXLpeIlwdiCxZjDX[ZN1KE6rbHWgeolzfXNiVlzQJJJmeGyrY3;uJIlvKEKKS{KxJINmdGxibHnu[UwhTUN3ME2xMlEh|ryP MkLNNVY2Ozl2MEK=
HeLa cell MUXGeY5kfGmxbjDhd5NigQ>? MV7F[oZm[3SrdnWgZ49v[2WwdILheIlwdiC{ZYH1bZJm\CC2bzDpcohq[mm2IILld5BqemG2b4L5JJN6dmGydHnhcEhTW1ZrII\pdpV{NWmwZIXj[YQh[3m2b4DheIhq[2m2eTDifUA2OCViaX6gTIVN[SClZXzsJIxqdmW|LDDFR|UxRTFwNTFOwG0> NFjZZlAyOTR7NUW4Oi=>
MDCK cells MX\GeY5kfGmxbjDhd5NigQ>? NYLwSWxsOzZiaB?= NWXV[3kzSW62aXnu[ox2\W68YTDhZ5Rqfmm2eTDh[4FqdnO2IHnu[ox2\W68YTDBJJZqenW|IFixUlEhcW6oZXP0[YQhcW5iTVTDT{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdpV{NWmwZIXj[YQh[3m2b4DheIhq[yCnZn\lZ5Qh[W[2ZYKgN|YhcHK|LDDJR|UxRTFwOTFOwG0> MlriNlI{PDF7NEO=
MDBK cells MVrGeY5kfGmxbjDhd5NigQ>? NV22WY9tOiCmYYnz M2HIV2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGJwfmmwZTD2bZJidCCmaXHydohm[SC4aYL1d{AyNU6DRFygRXRESyCYUkWzOEBqdm[nY4Tl[EBqdiCPRFLLJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBw\iC4aYLhcEBkgXSxcHH0bIlkKGWoZnXjeEBi\nSncjCyJIRigXNiYoSgUXRUKGG|c3H5MEBGSzVyPUSuOkDPxE1? MY[yNlk1PDN|Mx?=
african green monkey Vero cells MlHrSpVv[3Srb36gZZN{[Xl? NUW2NmVrPSCmYYnz NXXxNolmSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhWlOYIFGyJIlv\mWldHXkJIlvKGGocnnjZY4h\3KnZX6gcY9vc2W7IG\ldo8h[2WubIOgZYZ1\XJiNTDkZZl{KGK7IIDsZZF2\SC{ZXT1Z5Rqd25iYYPzZZktKEWFNUC9O{DPxE1? NVjjN4ozOjB|NUm4PVg>
HEL cells NFfn[3JHfW6ldHnvckBie3OjeR?= MkiwNk0{KGSjeYO= MUnBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDI[ZJx\XNic3ntdIxmgCC4aYL1d{AyKEuRUzDpcoZm[3SnZDDpckBJTUxiY3XscJMh[XO|ZYPz[YQh[XNicILveIVkfGmxbjDmdo9uKH[rcoXzMYlv\HWlZXSgZ5l1d3CjdHjv[4VvcWOrdImgZYZ1\XJiMjD0c{A{KGSjeYOgZpkhVVSWIHHzd4F6NCCHQ{WwQVExKM7:TR?= NFW4b4ozODB|NEexNS=>
HEK293 cells M1j1[2Z2dmO2aX;uJIF{e2G7 NEC5UHczPCCq M{fTfmlvcGmkaYTpc44hd2ZiaX7mcJVmdnqjIFGgeolzfXNiUl7BMYRmeGWwZHXueEBTVkFicH;sfY1memG|ZTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCmdXHsJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0yPSEQvF2= M1T6NFI1OzF|N{Ow
human BE(2)-C cells M3jlN2Z2dmO2aX;uJIF{e2G7 NXfibGZEOThvMkCgbC=> M1f0fWFvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JHdme3Sncn6g[ZF2cW6nIHXuZ4VxcGGub335[YxqfGm|II\pdpV{KGmwZnXjeIVlKGmwIHj1cYFvKEKHKEKpMWMh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCCUTlGgdoVxdGmlYYTpc44h[W[2ZYKgNVghfG9iMkCgbJJ{KGK7IHz1Z4ln\XKjc3WgdoVxd3K2ZYKg[4Vv\SCjc4PhfUwhUUN3ME2xOkDPxE1? NXf2bXJYOjRzNUG5OVQ>
human HuH7-J20 cells NHPG[WhHfW6ldHnvckBie3OjeR?= NWHU[FZjSW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEOYIFrGTFEhcW6oZXP0[YQhcW5iaIXtZY4hUHWKNz3KNlAh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCCuaX\lJIN6[2ynIHL5JI1m[XO3cnnu[{B{\WO{ZYTl[EBidGujbHnu[UBxcG:|cHjheIF{\SCuZY\lcEBxemWrbnP1ZoF1\WRid3n0bEBk\WyuczDmc5IhOSCqcjDmc4xtd3enZDDifUB3cXKjbDDpco9kfWyjdHnvckBnd3JiMzDodpMhdWWjc4Xy[YQh[XRiN{KgbJJ{NCCHQ{WwQVMxNjRizszN NWD3ZoJNOjZ2Mki4O|A>
human MT4 cells NGCzfXpEgXSxdH;4bYPDqGG|c3H5 NYLubpZFQTZiaB?= NWexO5VOS3m2b4TvfIlkcXS7IHHnZYlve3RiaIXtZY4hVVR2IHPlcIx{KGGodHXyJFk3KGi{czDifUBOXFRiYYPzZZktKEOFNUC9N|Eh|ryP MoXiNVk1QDJ2OEG=
human HuH7 cells M3X4fWN6fG:2b4jpZ:Kh[XO|YYm= M3HJPFMh\GG7cx?= Ml;UR5l1d3SxeHnjbZR6KGGpYXnud5QhcHWvYX6gTJVJPyClZXzsd{Bqdm[nY4Tl[EB4cXSqIFjDWlFjKGGodHXyJFMh\GG7czDifUBOXFNiYYPzZZktKEOFNUC9N|Ih|ryP M3GyU|IzOTByMkW2
human KB cells MmHpSpVv[3Srb36gZZN{[Xl? MXG3NkBp MWjBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDSbIlvd3[rcoXzJJR6eGViMUOgbY5n\WO2ZXSgbY4hcHWvYX6gT2Ih[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJ2ey2rbnT1Z4VlKGO7dH;wZZRpcWNiZX\m[YN1KGGodHXyJFczKGi{czDy[YxifGm4ZTD0c{Bkd262cn;s MWS3NlI4OzB?
human KB cells NV7SU2JxTnWwY4Tpc44h[XO|YYm= MXmxMVExODBizsznM41t MX23NkBp NEC4bFRCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBTcGmwb4\pdpV{KHS7cHWgNVMhcW6oZXP0[YQhcW5iaIXtZY4hU0JiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXK3cz3pcoR2[2WmIHP5eI9x[XSqaXOg[YZn\WO2IHH0JFEhfG9iMUCwNEB2\y:vbDDh[pRmeiB5MjDodpM> NE\HcXUzOTF{M{S=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
p53 / p-p53 / p21 / Mdm2 ; 

PubMed: 22962590     


Dose-dependent expression and phosphorylation of p53 induced by ribavirin. HepG2 cells were treated with the indicated doses of ribavirin (0, 5, 10, 30, 100 µg/ml) for 24 h.

EZH2 / p-ERK / ERK / p-eIF4E / eIF4E / p21 ; 

PubMed: 29487714     


Western blot analyses of EZH2, ERK1/2, eIF4E, and p21 expression in BT12, BT16, and BT37 cells 72 and 96hrs after ribavirin treatment. 

Cyclin D1 ; 

PubMed: 21415224     


Cyclin D1, NBS1 and VEGF protein levels were assessed by western blot in MDA-MB-468 and BT474 cells, treated with the indicated concentrations of ribavirin for 3 days. 

p-AKT / AKT / pBP1 / BP1 ; 

PubMed: 21415224     


AKT activity was assessed as the level of phosphorylation of AKT and its downstream target 4E-BP1 in MDA-MB-468 and BT474 cells treated with the indicated concentrations of ribavirin for 3 days. 

22962590 29487714 21415224
Growth inhibition assay
Cell number ; 

PubMed: 21415224     


Breast cancer cell lines were cultured in the absence or presence of ribavirin at the indicated concentrations for 7 days, and the number of cells was determined.

21415224

Protocol

Solubility (25°C)

In vitro DMSO 49 mg/mL (200.64 mM)
Water 49 mg/mL (200.64 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 244.20864
Formula

C8H12N4O5

CAS No. 36791-04-5
Storage powder
in solvent
Synonyms NSC-163039, ICN-1229 ,RTCA, Tribavirin

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03889106 Recruiting Drug: Ribavirin Lassa Fever University of Oxford|National Institute for Health Research United Kingdom|Kenema Government Hospital|London School of Hygiene and Tropical Medicine|Public Health England March 1 2019 --
NCT03585725 Recruiting Drug: Ribavirin Follicular Lymphoma|Mantle Cell Lymphoma Weill Medical College of Cornell University September 26 2018 Early Phase 1
NCT02940691 Terminated Drug: Grazoprevir/elbasvir Hepatitis C Kirby Institute May 1 2017 Phase 4
NCT02945150 Active not recruiting Drug: grazoprevir plus elbasvir (fixed dose combination) Renal Failure Massachusetts General Hospital|Merck Sharp & Dohme Corp. February 1 2017 Phase 4
NCT03004625 Completed Drug: daclatasvir|Drug: asunaprevir|Drug: Ribavirin Hepatitis C Kaohsiung Medical University Chung-Ho Memorial Hospital|Chang Gung Memorial Hospital|National Taiwan University Hospital|Taipei Veterans General Hospital Taiwan|China Medical University Hospital|National Cheng-Kung University Hospital November 2016 Phase 3

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID