Ribavirin (ICN-1229)

Catalog No.S2504 Synonyms: NSC-163039, RTCA, Tribavirin

For research use only.

Ribavirin (NSC-163039, ICN-1229, RTCA, Tribavirin), a synthetic guanosine analogue, possesses a broad spectrum of activity against DNA and RNA viruses.

Ribavirin (ICN-1229) Chemical Structure

CAS No. 36791-04-5

Selleck's Ribavirin (ICN-1229) has been cited by 17 publications

Purity & Quality Control

Choose Selective Antiviral Inhibitors

Biological Activity

Description Ribavirin (NSC-163039, ICN-1229, RTCA, Tribavirin), a synthetic guanosine analogue, possesses a broad spectrum of activity against DNA and RNA viruses.
In vitro

Ribavirin significantly reduces the efficiency with which progeny subgenomic replicons transfect new cells in the replicon system, although ribavirin has little effect on levels of HCV replication. Ribavirin increases the mutation frequency of HCV, with the highest rates of mutations being found in the NS5A-encoding region. Ribavirin enhances TH1 while inhibiting T 2 cytokine production by stimulated T cells. [1] Ribavirin shows antiviral activity against a variety of RNA viruses and is used in combination with interferon-alpha to treat hepatitis C virus infection. Ribavirin reduces infectious poliovirus production to as little as 0. 00001% in cell culture. [2] Ribavirin's antiviral activity is exerted directly through lethal mutagenesis of the viral genetic material. [3] Ribavirin markedly reduces viral-induced parameters of macrophage activation at physiologic concentrations (up to 500 mg/mL). Ribavirin inhibits the production of IL-4 by Th2 cells, whereas it does not diminish the production of IFN-gamma in Th1 cells. [4] Ribavirin exhibits antiviral activity against a broad range of both DNA and RNA viruses in vitro. Ribavirin is a cytostatic agent and causes a reduction in synthesis of DNA, RNA and proteins in exposed cells. Ribavirin is thought to induce a switch in T-helper cell phenotype from type 2 to type 1. [5]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
E6SM cell lines NIq2N3lHfW6ldHnvckBie3OjeR?= M{HYNGVn\mWldHn2[UBkd26lZX70doF1cW:wIILldZVqemWmIITvJIlvcGmkaYSgTIVzeGW|IIPpcZBt\XhidnnyeZMuOSiKU2[tNUkhNyC2aIntbY5mKGurbnHz[UBl\W[rY3nlcpQhMFSNLTnCNlAxPiBxIG\NW|E5OzdiaX7keYNm\CCleYTvdIF1cGmlaYT5JIJ6KDVyJTDpckBGPlOPIHPlcIwhdGmwZYOsJGVEPTB;MD6wNFUh|ryP NGq2bGgyOTR7NUW4Oi=>
MDCK cells NH\ZT5dHfW6ldHnvckBie3OjeR?= NETBNGY{KGSjeYO= MkjSRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgTY5ndHWnborhJGIhfmm{dYOgLGIwUEtxNT:3NkkhcW6oZXP0[YQhcW5iTVTDT{Bk\WyuczDhd5Nme3OnZDDhd{B3cXK3cz3pcoR2[2WmIHP5eI9x[XSqaXOg[YZn\WO2IHHmeIVzKDNiZHH5d{BjgSCvaXPyc5Nkd3CrYzDhcoFtgXOrczygSWM2OD1yLkC5OEDPxE1? NWjEfpp6OjNzMUexO|M>
mouse L1210 cells NGDH[3FHfW6ldHnvckBie3OjeR?= MYSyNE03OCCvaX7z MVTJcohq[mm2aX;uJI9nKEmPUFTIJIlvKG2xdYPlJGwyOjFyIHPlcIx{KGG|c3Xzd4VlKGG|IH\vdo1ifGmxbjDv[kBcOix6LUPIYYh6eG:6YX70bIlv\SCocn;tJHszNDhvM1jdTW1RKGGodHXyJFIxKHSxIE[wJI1qdnNuIFnDOVA:OSEQvF2= M4TEVFIzPTV3MUWy
human CEM cells NFrxN4FHfW6ldHnvckBie3OjeR?= MkHqNlAuPjBibXnudy=> NXTjflV1UW6qaXLpeIlwdiCxZjDJUXBFUCCrbjDoeY1idiCFRV2gZ4VtdHNiYYPz[ZN{\WRiYYOg[o9zdWG2aX;uJI9nKFt{LEitN2hecHmyb4jhcpRpcW6nIH\yc40hYzJuOD2zTH1KVVBiYX\0[ZIhOjBidH:gOlAhdWmwczygTWM2OD1zIN88US=> M1nkV|IzPTV3MUWy
BHK21 cell line MUHGeY5kfGmxbjDhd5NigQ>? MojFTY5pcWKrdHnvckBw\iCZZYP0JG5qdGVidnnyeZMhXkySIILldIxq[2:wIHnuJGJJUzJzIHPlcIwhdGmwZTygSWM2OD1zLkGg{txO NYnjSXNuOTZ3M{m0NFI>
HeLa cell NHzW[4lHfW6ldHnvckBie3OjeR?= NITpSGFG\m[nY4TpeoUh[2:wY3XueJJifGmxbjDy[ZF2cXKnZDD0c{BqdmirYnn0JJJme3CrcnH0c5J6KHO7bnHweIlidCiUU2[pJJZqenW|LXnu[JVk\WRiY4n0c5BifGirY3n0fUBjgSB3MDWgbY4hUGWOYTDj[YxtKGyrbnXzMEBGSzVyPUGuOUDPxE1? MoXNNVE1QTV3OE[=
MDCK cells NHrUWI1HfW6ldHnvckBie3OjeR?= NYrFfmk3OzZiaB?= Mkm2RY51cWmwZnz1[Y57[SCjY4Tpeol1gSCjZ3HpcpN1KGmwZnz1[Y57[SCDII\pdpV{KEhzTkGgbY5n\WO2ZXSgbY4hVUSFSzDj[YxteyCjc4Pld5Nm\CCjczDpcohq[mm2aX;uJI9nKH[rcoXzMYlv\HWlZXSgZ5l1d3CjdHjpZ{Bm\m[nY4SgZYZ1\XJiM{[gbJJ{NCCLQ{WwQVEvQSEQvF2= MojaNlI{PDF7NEO=
MDBK cells NEHvT2JHfW6ldHnvckBie3OjeR?= NInQVXUzKGSjeYO= M{n0V2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGJwfmmwZTD2bZJidCCmaXHydohm[SC4aYL1d{AyNU6DRFygRXRESyCYUkWzOEBqdm[nY4Tl[EBqdiCPRFLLJINmdGy|IHHzd4V{e2WmIHHzJJJm\HWldHnvckBw\iC4aYLhcEBkgXSxcHH0bIlkKGWoZnXjeEBi\nSncjCyJIRigXNiYoSgUXRUKGG|c3H5MEBGSzVyPUSuOkDPxE1? M1Lu[FIzQTR2M{Oz
african green monkey Vero cells NXH0OJlUTnWwY4Tpc44h[XO|YYm= MX:1JIRigXN? NIGxUG1CdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBTW1ZiQUKgbY5n\WO2ZXSgbY4h[W[{aXPhckBoemWnbjDtc45s\XliVnXyc{Bk\WyuczDh[pRmeiB3IHThfZMh[nlicHzhdZVmKHKnZIXjeIlwdiCjc4PhfUwhTUN3ME23JO69VQ>? MkTLNlA{PTl6OUi=
HEL cells M361RmZ2dmO2aX;uJIF{e2G7 NIHvW|AzNTNiZHH5dy=> M4rLT2FvfGm4aYLhcEBi[3Srdnn0fUBi\2GrboP0JGhmenCnczDzbY1xdGW6II\pdpV{KDFiS1;TJIlv\mWldHXkJIlvKEiHTDDj[YxteyCjc4Pld5Nm\CCjczDwdo91\WO2aX;uJIZzd21idnnyeZMucW6mdXPl[EBkgXSxcHH0bI9o\W6rY3n0fUBi\nSncjCyJJRwKDNiZHH5d{BjgSCPVGSgZZN{[XluIFXDOVA:OTBizszN MkLRNlAxOzR5MUG=
HEK293 cells MnfSSpVv[3Srb36gZZN{[Xl? M37qXlI1KGh? M2K4OWlvcGmkaYTpc44hd2ZiaX7mcJVmdnqjIFGgeolzfXNiUl7BMYRmeGWwZHXueEBTVkFicH;sfY1memG|ZTDlfJBz\XO|ZXSgbY4hUEWNMkmzJINmdGy|IHHmeIVzKDJ2IHjyd{BjgSCmdXHsJIx2[2moZYLhd4UhemWyb4L0[ZIh\2WwZTDhd5NigSxiRVO1NF0yPSEQvF2= M{\RRlI1OzF|N{Ow
human BE(2)-C cells MV3GeY5kfGmxbjDhd5NigQ>? NFvjRpAyQC1{MDDo MlKwRY51cX[rcnHsJIFkfGm4aYT5JIFo[Wmwc4SgW4V{fGW{bjDldZVqdmViZX7j[ZBp[WyxbYnlcIl1cXNidnnyeZMhcW6oZXP0[YQhcW5iaIXtZY4hSkVqMjmtR{Bk\WyuczDhd5Nme3OnZDDhd{BqdmirYnn0bY9vKG:oII\pdoFtKFKQQTDy[ZBtcWOjdHnvckBi\nSncjCxPEB1dyB{MDDodpMh[nlibIXjbYZmemG|ZTDy[ZBwenSncjDn[Y5mKGG|c3H5MEBKSzVyPUG2JO69VQ>? NF3UWnQzPDF3MUm1OC=>
human HuH7-J20 cells NVrsdYk4TnWwY4Tpc44h[XO|YYm= NXLGWlc2SW62aY\pdoFtKGGldHn2bZR6KGGpYXnud5QhUEOYIFrGTFEhcW6oZXP0[YQhcW5iaIXtZY4hUHWKNz3KNlAh[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJidCCuaX\lJIN6[2ynIHL5JI1m[XO3cnnu[{B{\WO{ZYTl[EBidGujbHnu[UBxcG:|cHjheIF{\SCuZY\lcEBxemWrbnP1ZoF1\WRid3n0bEBk\WyuczDmc5IhOSCqcjDmc4xtd3enZDDifUB3cXKjbDDpco9kfWyjdHnvckBnd3JiMzDodpMhdWWjc4Xy[YQh[XRiN{KgbJJ{NCCHQ{WwQVMxNjRizszN M2r0NFI3PDJ6OEew
human MT4 cells MofFR5l1d3SxeHnjxsBie3OjeR?= M{TYcFk3KGh? NH\vcJdEgXSxdH;4bYNqfHliYXfhbY5{fCCqdX3hckBOXDRiY3XscJMh[W[2ZYKgPVYhcHK|IHL5JG1VXCCjc4PhfUwhS0N3ME2zNUDPxE1? NXi1cFl2OTl2OEK0PFE>
human HuH7 cells NYn0XpZjS3m2b4TvfIlkyqCjc4PhfS=> NGewPWk{KGSjeYO= M1PlcGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGh2UDdiY3XscJMhcW6oZXP0[YQhf2m2aDDIR3Yy[iCjZoTldkA{KGSjeYOgZpkhVVSVIHHzd4F6NCCFQ{WwQVMzKM7:TR?= NW[0[o9DOjJzMECyOVY>
human KB cells M3GyR2Z2dmO2aX;uJIF{e2G7 MYO3NkBp NIPuSWpCdnSrdnnyZYwh[WO2aY\peJkh[WejaX7zeEBTcGmwb4\pdpV{KHS7cHWgNVMhcW6oZXP0[YQhcW5iaIXtZY4hU0JiY3XscJMh[XO|ZYPz[YQh[XNiaX7obYJqfGmxbjDv[kB3cXK3cz3pcoR2[2WmIHP5eI9x[XSqaXOg[YZn\WO2IHHmeIVzKDd{IHjyd{Bz\WyjdHn2[UB1dyClb370do9t NWPkPJdVPzJ{N{Ow
human KB cells NHu2eGtHfW6ldHnvckBie3OjeR?= NUTvNGo1OS1zMECwJO69\y:vbB?= MoTyO|IhcA>? MYTBcpRqfmm{YXygZYN1cX[rdImgZYdicW6|dDDSbIlvd3[rcoXzJJR6eGViMUOgbY5n\WO2ZXSgbY4hcHWvYX6gT2Ih[2WubIOgZZN{\XO|ZXSgZZMhcW6qaXLpeIlwdiCxZjD2bZJ2ey2rbnT1Z4VlKGO7dH;wZZRpcWNiZX\m[YN1KGG2IEGgeI8hOTByMDD1[{9udCCjZoTldkA4OiCqcoO= MlXBNlEyOjN2
Assay
Methods Test Index PMID
Western blot p53 / p-p53 / p21 / Mdm2 ; EZH2 / p-ERK / ERK / p-eIF4E / eIF4E / p21 ; Cyclin D1 ; p-AKT / AKT / pBP1 / BP1 22962590 29487714 21415224
Growth inhibition assay Cell number 21415224

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 49 mg/mL
(200.64 mM)
Water 49 mg/mL
(200.64 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 244.20864
Formula

C8H12N4O5

CAS No. 36791-04-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=NC(=NN1C2C(C(C(O2)CO)O)O)C(=O)N

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04283513 Not yet recruiting Drug: Virazole Hemorrhagic Fever U.S. Army Medical Research and Development Command June 2021 Phase 2
NCT04335123 Completed Drug: Losartan COVID-19 University of Kansas Medical Center April 4 2020 Phase 1
NCT04285034 Completed -- Lassa Fever University of Oxford November 26 2019 --
NCT03889106 Unknown status Drug: Ribavirin Lassa Fever University of Oxford|National Institute for Health Research United Kingdom|Kenema Government Hospital|London School of Hygiene and Tropical Medicine|Public Health England March 1 2019 --
NCT03585725 Terminated Drug: Ribavirin Follicular Lymphoma|Mantle Cell Lymphoma Weill Medical College of Cornell University|Memorial Sloan Kettering Cancer Center September 26 2018 Early Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

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