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Indocyanine green

Name: Indocyanine green
Brand: Selleck Chemicals
SKU: S1924
Availability: InStock
Rating: 5 (3 reviews)

Cat.No.S1924

Indocyanine green(Cardiogreen) is a cyanine dye that binds to plasma proteins and lipoproteins. It is used in medical diagnostics. It is used for determining cardiac output, hepatic function, and liver blood flow, and for ophthalmic angiography. Indocyanine green-photodynamic therapy(ICG-PDT) induces apoptosis in HGF cells at transcriptome level.

Chemical Structure

Molecular Weight: 774.96

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Quality Control

Batch: Purity: 98.80%

98.80

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Solubility

In vitro
Batch:

DMSO : 100 mg/mL (129.03 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.500mg/ml (1.94mM)	Taking the 1 mL working solution as an example, add 50 μL of 30 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.500mg/ml (1.94mM)	Taking the 1 mL working solution as an example, add 50 μL of 30 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	774.96	Formula	C₄₃H₄₇N₂NaO₆S₂	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	3599-32-4	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Cardiogreen			SMILES	CC1(C(=[N+](C2=C1C3=CC=CC=C3C=C2)CCCCS(=O)(=O)[O-])C=CC=CC=CC=C4C(C5=C(N4CCCCS(=O)(=O)[O-])C=CC6=CC=CC=C65)(C)C)C.[Na+]

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

References	[1] https://pubmed.ncbi.nlm.nih.gov/28438509/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-07-01)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT07677839	RECRUITING	Breast Cancer	University of California, Davis	2026-06-17
NCT07635420	NOT_YET_RECRUITING	Head and Neck Cancer (H&N); Free Tissue Flaps; Free Flap; Free Flap Evaluation; Indocyanine Green; Indocyanine Green (ICG); Microsurgery; Randomised Controlled Trial	Region Skane	2026-10-01	PHASE4
NCT07060443	RECRUITING	Right Hemicolectomy	Pusan National University Yangsan Hospital	2025-07-23	PHASE2
NCT06610071	NOT_YET_RECRUITING	Dermatofibrosarcoma Protuberans (DFSP); Skin Squamous Cell Carcinoma	The Plastic Surgery Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College	2024-10-30	PHASE1
NCT06638125	NOT_YET_RECRUITING	Non Small Cell Lung Cancer	St. Joseph's Healthcare Hamilton	2026-12-01	PHASE1; PHASE2
NCT05350540	RECRUITING	Surgery Site Fistula	University Health Network, Toronto	2021-11-11	PHASE2

Tech Support

Handling Instructions

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