CAS No. 3599-32-4

Batch: Purity:98.80
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Indocyanine green Chemical Structure
Chemical Structure
Check the Indocyanine green product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name sodium 4-[(2Z)-2-[(2E,4E,6E)-7-[1,1-dimethyl-3-(4-sulfonatobutyl)benzo[e]indol-3-ium-2-yl]hepta-2,4,6-trienylidene]-1,1-dimethylbenzo[e]indol-3-yl]butane-1-sulfonate
CAS Number 3599-32-4
Molecular Formula

C43H47N2NaO6S2
Molecular Weight (MW) 774.96
SMILES CC1(C(=[N+](C2=C1C3=CC=CC=C3C=C2)CCCCS(=O)(=O)[O-])C=CC=CC=CC=C4C(C5=C(N4CCCCS(=O)(=O)[O-])C=CC6=CC=CC=C65)(C)C)C.[Na+]
InChIKey MOFVSTNWEDAEEK-UHFFFAOYSA-M

Ordering Information

Biological Activity

Indocyanine green (Cardiogreen) is a cyanine fluorescent dye used as a diagnostic contrast agent. It binds tightly to plasma proteins and remains largely confined to the vascular system, exhibiting peak spectral absorption at approximately. Upon administration, it is rapidly cleared from circulation exclusively by hepatocytes into bile, enabling the assessment of cardiac output, hepatic function, and retinal vascular imaging, as well as triggering photodynamic cell apoptosis.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 100 mg/mL (129.03 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water Insoluble
Ethanol Insoluble
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.