Home DNA Damage/DNA Repair Antineoplastic and Immunosuppressive Antibiotics inhibitor Carboplatin

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Carboplatin DNA Synthesis inhibitor

Cat.No.S1215

Carboplatin, a derivative of CDDP, is a DNA synthesis inhibitor which binds to DNA, inhibits replication and transcription and induces cell death and interfers with the cell's repair mechanism in A2780, SKOV-3, IGROV-1, and HX62 cells. It induces the formation of DNA interstrand crosslinks and DNA-protein crosslinks. Solutions are unstable and should be fresh-prepared.DMSO is not recommended to dissolve platinum-based drugs, which can easily lead to drug inactivation.
Carboplatin Antineoplastic and Immunosuppressive Antibiotics inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 371.25

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Quality Control

Batch: Purity: 99.98%
99.98

Cell Culture, Treatment & Working Concentration

Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
Tca8113/ut  Growth Inhibition Assay 2-25 μM 72 h inhibits cell viability dose dependently 26137105
Tca8113/CBP  Growth Inhibition Assay 2-25 μM 72 h inhibits cell viability dose dependently 26137105
Tca8113/PYM  Growth Inhibition Assay 2-25 μM 72 h inhibits cell viability dose dependently 26137105
SKOV3 Growth Inhibition Assay 60 mg/ml 1-5 d inhibits cell viability time dependently 26137043
SKOV3 Growth Inhibition Assay 0-4 μg/ml 24/48/72 h inhibits cell viability dose and time dependently 26125273
SKOV3 Apoptosis Assay 2 μg/ml 48 h induces apoptosis 26125273
OSA Clonogenic Assay 2.5/5 μM results in lower colony formation as compared to either BMI1 siRNA treatment 26110620
A549 Fuction Assay 0/25/50 μM 24 h downregulates miR-205 expression  25917317
H1975 Fuction Assay 0/25/50 μM 24 h downregulates miR-205 expression  25917317
A549 Fuction Assay 0/25/50 μM 24 h upregulates miR-218 expression 25917317
H1975 Fuction Assay 0/25/50 μM 24 h upregulates miR-218 expression 25917317
A549 Growth Inhibition Assay 25 μM 24 h inhibits cell growth 25917317
H1975 Growth Inhibition Assay 25 μM 24 h inhibits cell growth 25917317
MDA-MB-231 Growth Inhibition Assay 0-220 μg/ml 72 h IC50 = 86 μM 25837691
T47D Growth Inhibition Assay 0-220 μg/ml 72 h IC50 = 48.9 μM 25837691
LCTCC Growth Inhibition Assay IC50=3.8 µM 25773167
MCTCC Growth Inhibition Assay IC50=2.8 µM 25773167
MegTCC Growth Inhibition Assay IC50=4.1 µM 25773167
MonoTCC Growth Inhibition Assay IC50=5.6 µM 25773167
MCF7 Growth Inhibition Assay 20 μM 72 h induces cell death of HP1β-depleted MCF7 cells 25769025
A-549  Growth Inhibition Assay 0.5 μM 24 h DMSO  inhibits cell growth significantly 25755690
A-549  Apoptosis Assay 0.5 μM 48 h DMSO  shows early apoptosis features including blebbing and chromatin condensation 25755690
A-549  Fuction Assay 0.5 μM 48 h DMSO  shows aberrant microtubule disruption 25755690
A-549  Fuction Assay 0.5 μM 48 h DMSO  inhibits of cell migration  25755690
RMG-1 Growth Inhibition Assay IC50=28.8 ± 3.6 mg/L 25726913
FN-RMG-1 Growth Inhibition Assay IC50=40.5 ± 4.2 mg/L μM 25726913
RMG-1-hFUT Growth Inhibition Assay IC50=58.1 ± 2.4 mg/L 25726913
FN-RMG-1-hFUT Growth Inhibition Assay IC50=118.8 ± 13.8 mg/L 25726913
CHP-134  Growth Inhibition Assay 1 μM DMSO  inhibits significant cell growth combined with GSK2830371 25658463
IMR-32 Growth Inhibition Assay 1 μM DMSO  inhibits significant cell growth combined with GSK2830371 25658463
CHP-134  Fuction Assay 10 μM  DMSO  induces apoptosis significantly combined with GSK2830371 25658463
IMR-32 Fuction Assay 10 μM  DMSO  induces apoptosis significantly combined with GSK2830371 25658463
A549 Growth Inhibition Assay 0.2-4000 μM 72 h IC50=126 ± 5.0 μM 25625243
A549/CDDP Growth Inhibition Assay 0.2-4000 μM 72 h IC50=240 ± 45.7 μM 25625243
H460  Growth Inhibition Assay 24 h DMSO IC50=7.9 μM 25599995
H460  Growth Inhibition Assay 7.9 μM 24 h DMSO shows a radiosensitizing effect to X-rays 25599995
NRK-52E Growth Inhibition Assay 72 h  IC50=100 ± 12.9 μM 25565603
RGE Growth Inhibition Assay 72 h  IC50=22 ± 7.4 μM 25565603
NRK-52E Growth Inhibition Assay 72 h  IC80=179 ± 9.5 μM 25565603
RGE Growth Inhibition Assay 72 h  IC80=63 ± 7.4 μM 25565603
SK-OV-3 Growth Inhibition Assay 72 h IC50=104.8 μM 25520132
ES-2 Growth Inhibition Assay 72 h IC50=14.6 μM 25520132
OVCAR-8 Growth Inhibition Assay 72 h IC50=96.0 μM 25520132
3AO Growth Inhibition Assay 72 h IC50=63.4 μM 25520132
A2780 Growth Inhibition Assay 72 h IC50=145.7 μM 25520132
SW626 Growth Inhibition Assay 72 h IC50=97.0 μM 25520132
OVCAR-3 Growth Inhibition Assay 72 h IC50=32.6 μM 25520132
A549 Growth Inhibition Assay IC50=151.56 ± 53.06 μM 25277461
4T1 Growth Inhibition Assay IC50=84.62 ± 30.05 μM μM 25277461
BALB/3T3 Growth Inhibition Assay IC50=14.30 ± 6.68 μM 25277461
B16 Growth Inhibition Assay IC50=64.30 ± 6.81 μM 25277461
DU 145 Growth Inhibition Assay IC50=35.04 ± 17.54 μM 25277461
FaDu Growth Inhibition Assay IC50=45.39 ± 11.50 μM 25277461
HCV-29T Growth Inhibition Assay IC50=48.57 ± 22.49 μM 25277461
HL-60 Growth Inhibition Assay IC50=11.10 ± 4.47 μM 25277461
HT-29 Growth Inhibition Assay IC50=89.16 ± 18.86 μM 25277461
H146 Growth Inhibition Assay IC50=49.6±13.1 μM 25124282
H187 Growth Inhibition Assay IC50=12.7±2.7 μM 25124282
H128 Growth Inhibition Assay IC50=106.4±9.6 μM 25124282
H69 Growth Inhibition Assay IC50=76.9±27.8 μM 25124282
H209 Growth Inhibition Assay IC50=258.1±99.8 μM 25124282
DMS153 Growth Inhibition Assay IC50=57.8±9.2 μM 25124282
H526 Growth Inhibition Assay IC50=7.2±1.7 μM 25124282
DMS114 Growth Inhibition Assay IC50=85.8±12.8 μM 25124282
DMS53 Growth Inhibition Assay IC50=146.2±36.4 μM 25124282
HeLa Growth Inhibition Assay 5-80 μM inhibits cell growth dose dependently 25109360
MCF-7 Growth Inhibition Assay 5-80 μM inhibits cell growth dose dependently 25109360
MDA-MB-231 Growth Inhibition Assay 5-80 μM inhibits cell growth dose dependently 25109360
CaOV3 Growth Inhibition Assay 72 h IC50=86.1 μM 25068849
OVCAR-3 Growth Inhibition Assay 72 h IC50=179.5 μM 25068849
OVCAR-5 Growth Inhibition Assay 72 h IC50=75.7 μM 25068849
SKOV-3 Growth Inhibition Assay 72 h IC50=504 μM 25068849
OVCAR-4 Growth Inhibition Assay 72 h IC50=290.6 μM 25068849
OVCAR-8 Growth Inhibition Assay 72 h IC50=690.2 μM 25068849
MA148 Growth Inhibition Assay 24 h IC50=83.6 ± 1.4 μg/ml 24831091
A549 Growth Inhibition Assay 24 h IC50=776.0 ± 1.9 μg/ml 24831091
MDA-MB-231 Growth Inhibition Assay 24 h IC50=748.0 ± 1.1 μg/ml 24831091
NCI-ADR/RES  Growth Inhibition Assay 24 h IC50=1753.0 ± 4.4 μg/ml 24831091
MA148 Growth Inhibition Assay 48 h IC50=28.1 ± 1.0 μg/ml 24831091
A549 Growth Inhibition Assay 48 h IC50=118.0 ± 1.2 μg/ml 24831091
MDA-MB-231 Growth Inhibition Assay 48 h IC50=183.0 ± 1.2 μg/ml 24831091
NCI-ADR/RES  Growth Inhibition Assay 48 h IC50=193.0 ± 1.9 μg/ml 24831091
MA148 Growth Inhibition Assay 72 h IC50=13.7 ± 1.2 μg/ml 24831091
A549 Growth Inhibition Assay 72 h IC50=64.3 ± 1.1 μg/ml 24831091
MDA-MB-231 Growth Inhibition Assay 72 h IC50=99.5 ± 1.1 μg/ml 24831091
NCI-ADR/RES  Growth Inhibition Assay 72 h IC50=37.4 ± 1.7 μg/ml 24831091
A549 Growth Inhibition Assay 72 h DMSO IC50=152.409 μM 24721323
NCI-H157 Growth Inhibition Assay 72 h DMSO IC50=89.977 μM 24721323
PC9 Growth Inhibition Assay 72 h GC50=71.6±9.5 µM 24618809
A549  Growth Inhibition Assay 72 h GC50=136±31.6 µM  24618809
PC9 Apoptosis Assay 100 µM 72 h induces apoptosis 24618809
A549  Apoptosis Assay 100 µM 72 h induces apoptosis 24618809
A549 Growth Inhibition Assay 24 h IC10=1.476 nM 24396437
H1299 Growth Inhibition Assay 24 h IC10=0.443 nM 24396437
HCC15 Growth Inhibition Assay 24 h IC10=0.346 nM 24396437
H157 Growth Inhibition Assay 24 h IC10=0.81 nM 24396437
A2780 Growth Inhibition Assay IC50=14.0 ± 1.4 μM 24209693
A2780cisR Growth Inhibition Assay IC50=48.9 ± 3.9 μM 24209693
A2780ZD0473R Growth Inhibition Assay IC50=64.6 ± 3.2 μM 24209693
SKOV-3 Growth Inhibition Assay IC50=43.4 ± 3.9 μM 24209693
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

Water : 4.5 mg/mL

Ethanol : Insoluble

Molarity Calculator

Mass Concentration Volume Molecular Weight
Dilution Calculator Molecular Weight Calculator

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

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%
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% ddH2O
% DMSO
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Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight 371.25 Formula

C6H12N2O4Pt

 Storage (From the date of receipt) 2 years 4°C/3 years -20°C(in the dark) powder
CAS No. 41575-94-4 Download SDF Storage of Stock Solutions Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.
Synonyms NSC 241240, JM-8, CBDCA Smiles C1CC(C1)(C(=O)O)C(=O)O.[NH2-].[NH2-].[Pt+2]

Mechanism of Action

Features
This product is not recommended to be dissolved in dimethylsulfoxide (DMSO).
Targets/IC50/Ki
DNA synthesis
(A2780, SKOV-3, IGROV-1, HX62 cells)
In vitro

Carboplatin exhibits an inhibitory effect on cell proliferation in a human ovarian cancer cell line panel, including A2780, SKOV3, and IGROV-1 cells with IC50 of 6.1 μM, 12.4 μM and 2.2 μM, respectively. This compound also shows the anti-proliferative activities in lung carcinoid cell line, such as UMC-11, H727, and H835 cells with IC50 of 36.4 μM, 3.4 μM and 35.8 μM, respectively.

In vivo

In A2780 tumor xenografts, Carboplatin (60 mg/kg via i.p.) given as single agents shows a modest antitumor effect with the relative tumor volumes on day 6 of 8.4 compared to the control of 11.9, and the day 6 tumor weights relative to control (T/C) of 67%. For the VC8 (Brca2-deficient) xenografts, this compound treatment delays tumor growth and reduces tumor mass by 42% compared to the vehicle group.

References
  • [4] https://pubmed.ncbi.nlm.nih.gov/15014014/
  • [5] https://pubmed.ncbi.nlm.nih.gov/21868512/
  • [6] https://pubmed.ncbi.nlm.nih.gov/24812268/
  • [7] https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034078/

Applications

Methods Biomarkers Images PMID
Western blot pChK-2 / Chk-2 p-p53(S15) / p-p53(S46) / p-p53(S392) / p53 p-p38 / p38 / Cleaved PARP / PARP
S1215-WB1
30049957
Immunofluorescence γH2AX
S1215-IF1
24348048
Growth inhibition assay Cell viability
S1215-viability1
24348048

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT06393816 Not yet recruiting
Large Cell Neuroendocrine Carcinoma of the Lung
Centre Leon Berard|Groupe Français de Pneumo-Cancérologie
 May 2024 Phase 2
NCT06303713 Not yet recruiting
Prostate Cancer|Metastatic Prostate Cancer|Metastatic Castration-resistant Prostate Cancer
Dana-Farber Cancer Institute|Novartis
 May 2024 Phase 1
NCT05846789 Recruiting
Metastatic Breast Cancer|Triple Negative Breast Cancer|Estrogen-receptor-low Breast Cancer
Kathy Miller|Genentech Inc.|Indiana University
 May 2024 Phase 2
NCT06288854 Not yet recruiting
Weight Gain
Rajavithi Hospital
 April 3 2024 Not Applicable

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
Can you advise what solvent to use and the concentration for it for in vitro use?

Answer:
DMSO will affect the activity of platinum agents. For in vitro use, according to our experience in dissolving this compound, we didn't find any solvent can dissolve it alone, but it can be dissolved in co-solvent ethanol:water=1:2 at 15 mg/ml. We didn't test the stability of the drug in such vehicle, so it is recommended to prepare the solution just before use.