Home DNA Damage/DNA Repair Antineoplastic and Immunosuppressive Antibiotics inhibitor Carboplatin

research use only

Carboplatin DNA Synthesis inhibitor

Cat.No.S1215

Carboplatin, a derivative of CDDP, is a DNA synthesis inhibitor which binds to DNA, inhibits replication and transcription and induces cell death and interfers with the cell's repair mechanism in A2780, SKOV-3, IGROV-1, and HX62 cells. It induces the formation of DNA interstrand crosslinks and DNA-protein crosslinks. Solutions are unstable and should be fresh-prepared.DMSO is not recommended to dissolve platinum-based drugs, which can easily lead to drug inactivation.
Carboplatin Antineoplastic and Immunosuppressive Antibiotics inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 371.25
Jump to

Quality Control

Batch: Purity: 99.98%
99.98

Solubility in DMSO or Water

In vitro
Batch:

Water : 4.5 mg/mL

Ethanol : Insoluble

The solubility data above are all experimental results (not literature values).

Molarity Calculator

Mass Concentration Volume Molecular Weight
Dilution Calculator Molecular Weight Calculator

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg
g
μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO
%
% Tween 80
% ddH2O
% DMSO
+
%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Read more about Carboplatin solubility in DMSO or water

Working Concentrations for Cell Culture Treatment

Step 1: Select a research area to find working concentrations, or search directly for a cell line

Read more about Carboplatin working concentrations for cell culture treatment

Working Concentrations for Animal Model Treatment

Step 1: Select a research area to find working concentrations, or search directly for an animal type

×

Mechanism of Action

Information Carboplatin is a cytotoxic platinum-based DNA cross-linking agent. It affects p53, DNA damage response, and apoptosis signaling pathways, by binding to DNA to form intra- and interstrand crosslinks and inducing severe DNA damage and ROS production, to inhibit cancer cell proliferation and promote apoptosis.

Read more about Carboplatin IC50 for cell-based and cell-free assays
Primary Applications and Mechanism of Action

Read more about Carboplatin Mechanism of Action

Chemical Information, Storage & Stability

Molecular Weight 371.25 Formula

C6H12N2O4Pt

 Storage (From the date of receipt) 2 years 4°C/3 years -20°C(in the dark) powder
CAS No. 41575-94-4 Download SDF Storage of Stock Solutions Solutions are unstable. Prepare fresh or purchase small, pre-packaged sizes. Repackage upon receipt.
Synonyms NSC 241240, JM-8, CBDCA Smiles C1CC(C1)(C(=O)O)C(=O)O.[NH2-].[NH2-].[Pt+2]

Read more about storage & stability

Please select the combination compounds (Multiple selections allowed)
Please select a combination compound first

Read more comparative analysis about Carboplatin

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Frequently Asked Questions

Question 1:
Can you advise what solvent to use and the concentration for it for in vitro use?

Answer:
DMSO will affect the activity of platinum agents. For in vitro use, according to our experience in dissolving this compound, we didn't find any solvent can dissolve it alone, but it can be dissolved in co-solvent ethanol:water=1:2 at 15 mg/ml. We didn't test the stability of the drug in such vehicle, so it is recommended to prepare the solution just before use.