Biochanin A

Catalog No.S2377 Synonyms: 4-Methylgenistein

For research use only.

Biochanin A (4-Methylgenistein), an O-methylated isoflavone from Trifolium pratense, inhibits protein tyrosine kinase (PTK) of epidermal growth factor receptor with IC50 values of 91.5 μM.

Biochanin A Chemical Structure

CAS No. 491-80-5

Selleck's Biochanin A has been cited by 5 Publications

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Biological Activity

Description Biochanin A (4-Methylgenistein), an O-methylated isoflavone from Trifolium pratense, inhibits protein tyrosine kinase (PTK) of epidermal growth factor receptor with IC50 values of 91.5 μM.
Targets
FAAH (rat) [2] FAAH (mouse) [1] FAAH (human) [2] EGFR [1]
1.4 μM 1.8 μM 2.4 μM 91.5 μM
In vitro

Biochanin A has been reported to inhibit protein tyrosine kinase (PTK) of epidermal growth factor receptor with IC50 values of 91.5 μM [1] Biochanin A inhibits the hydrolysis of 0.5 μM anandamide by mouse, rat and human fatty acid amide hydrolase (FAAH) with IC50 values of 1.8, 1.4 and 2.4 μM respectively. [2] Biochanin A inhibits both serum and EGF-stimulated growth of LNCaP and DU-145 cells (IC50 values from 8.0 to 27 micrograms/ml for serum and 4.3 to 15 micrograms/ml for EGF), but has no significant effect of the EGF receptor tyrosine autophosphorylation. [3]

In vivo LD50: Mice 63mg/kg (i.p.) [4]

Protocol (from reference)

Solubility (25°C)

In vitro

DMSO 57 mg/mL
(200.52 mM)
Ethanol 9 mg/mL
(31.66 mM)
Water Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 284.26
Formula

C16H12O5

CAS No. 491-80-5
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=CC=C(C=C1)C2=COC3=CC(=CC(=C3C2=O)O)O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

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