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Doxorubicin (DOX) HCl of Selleck (Japan)

Doxorubicin (DOX) HCl

Antineoplastic and Immunosuppressive Antibiotics inhibitor

Catalog No.S1208 Purity:99.82%

research use only

Doxorubicin (DOX) HCl is an antibiotic agent that inhibits human DNA topoisomerase II with IC50 of 2.67 μM. Doxorubicin reduces basal phosphorylation of AMPK. Doxorubicin is used in the concomitant treatment of HIV-infected patients but is found to be at high risk of HBV reactivation.This product may precipitate when dissolved in PBS solution. It is recommended to prepare the stock solution in pure water and dilute with either pure water or saline to obtain the working solution.Doxorubicin (Adriamycin) HCl can be used to induce animal models of kidney disease.

Doxorubicin (DOX) HCl Chemical Structure

Molecular Weight: 579.98

Purity & Quality Control

Batch: Purity: 99.82%

99.82

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Cell Culture and Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Formulation	Activity Description	PMID
SGC7901	Growth Inhibition Assay	10 μM	72 h	DMSO	GI50=0.55 μM	24686011
HLF	Growth Inhibition Assay	10 μM	72 h	DMSO	GI50=0.33 μM	24686011
16HBE	Growth Inhibition Assay	10 μM	72 h	DMSO	GI50=0.18 μM	24650643
BALB/3T3	Growth Inhibition Assay				IC50=0.5 μM	24631190
26-L5	Function Assay		6 h		Antiinvasive activity	24592993
CEM/ADR5000	Cytotoxic Assay			DMSO	Cytotoxicity against human multidrug-resistant CEM/ADR5000 cells expressing p-glycoprotein with IC50 of 23.27 μM	24561670
CCRF-CEM	Cytotoxic Assay			DMSO	IC50=0.009 μM	24561670
V79	Cytotoxic Assay	1.10 μM	72 h	DMSO	IC50=0.24 μM	24530030
MX1	Cytotoxic Assay	1.10 μM	72 h	DMSO	IC50=0.5 μM	24530030
L929	Cytotoxic Assay	1.10 μM	72 h	DMSO	IC50=0.16 μM	24530030
ME180	Cytotoxic Assay		48 h		IC50=0.39 μM	24487188
MGC803	Cytotoxic Assay		72 h		IC50=0.17 μM	24370114
CHP100	Cytotoxic Assay		72 h		IC50=0.79 μM	24251417
Bel7402	Growth Inhibition Assay		96 h	DMSO	IC50=0.5782 μM	24211639
SGC7901	Cytotoxic Assay				IC50=0.011 μM	24195466
MKN28	Growth Inhibition Assay		48 h		GI50=0.24 μM	24184076
Caco2	Growth Inhibition Assay		48 h		GI50=0.32 μM	24184076
MDA-MB-468	Growth Inhibition Assay	10 μM	72 h		GI50=0.085 μM	24163729
Caco2	Cytotoxic Assay	40 μM	24 h		Cytotoxicity	24099994
U2OS	Growth Inhibition Assay		72 h		IC50=0.3 μM	24095089
OVCAR8	Growth Inhibition Assay	20 μM	72 h		Antiproliferative activity	24095089
LNcaP	Function Assay	3 μM	24 h		Up-regulation of p53 expression	24095089
LNcaP	Function Assay	3 μM	24 h		Increase of cleaved caspase-9 level	24095089
Caov3	Growth Inhibition Assay		72 h		IC50=0.1 μM	24095089
AS49-RAW	Cytotoxic Assay	100 μM	48 h		IC50=32.78 μM	24077528
MDA-MB-435	Cytotoxic Assay	100 μM	48 h		IC50=0.15 μM	24028446
HUVEC	Function Assay	5 μM	2 h		Cellular uptake	24028446
MRC5	Growth Inhibition Assay		72 h		IC50=0.1 μM	24021462
HepG2	Function Assay	40 μg/mL	48 h	DMSO	Inhibition of TRK with IC50 of 0.0025 μM	24013411
HUVEC	Cytotoxic Assay		48 h		IC50=0.0807 μM	24012378
KB/HeLa	Cytotoxic Assay		45 h		EC50=0.25 μM	23988351
MDA-MB-231	Function Assay	5 μM	24 h		Antioxidant activity assessed as intramitochondrial ROS level	23973213
MDA-MB-231	Function Assay	5 μM	24 h		Antioxidant activity assessed as intracellular malonyldialdehyde level	23973213
MDA-MB-231	Function Assay	5 μM	24 h		Cellular uptake	23973213
MCF7	Function Assay	5 μM	24 h		Cellular uptake	23973213
MCF7	Function Assay	5 μM	24 h		Antioxidant activity assessed as intracellular malonyldialdehyde level	23973213
MCF7	Function Assay	5 μM	24 h		Cellular uptake	23973213
MCF7	Function Assay	5 μM	24 h		Antioxidant activity assessed as intramitochondrial ROS level	23973213
H9c2	Function Assay	5 μM	24 h		Cellular uptake	23973213
H9c2	Function Assay	5 μM	24 h		Antioxidant activity assessed as intracellular ROS level	23973213
H9c2	Function Assay	5 μM	24 h		Antioxidant activity assessed as intracellular malonyldialdehyde level	23973213
H9c2	Function Assay	5 μM	24 h		Antioxidant activity assessed as intramitochondrial ROS level	23973213
HaCaT	Growth Inhibition Assay	25 μg/mL	48 h		TGI50=0.4 μM	23876338
UO31	Cytotoxic Assay	100 μM	48 h		GI50=0.49 μM	23871905
UACC257	Cytotoxic Assay	100 μM	48 h		GI50=0.14 μM	23871905
U251	Cytotoxic Assay	100 μM	48 h		GI50=0.04 μM	23871905
TK10	Cytotoxic Assay	100 μM	48 h		GI50=0.18 μM	23871905
SR	Cytotoxic Assay	100 μM	48 h		GI50=0.03 μM	23871905
SNB75	Cytotoxic Assay	100 μM	48 h		GI50=0.07 μM	23871905
SNB19	Cytotoxic Assay	100 μM	48 h		GI50=0.04 μM	23871905
SN12C	Cytotoxic Assay	100 μM	48 h		GI50=0.07 μM	23871905
SK-MEL-28	Cytotoxic Assay	100 μM	48 h		GI50=0.21 μM	23871905
SF539	Cytotoxic Assay	100 μM	48 h		GI50=0.12 μM	23871905
RXF393	Cytotoxic Assay	100 μM	48 h		GI50=0.1 μM	23871905
PRMI8226	Cytotoxic Assay	100 μM	48 h		GI50=0.08 μM	23871905
OVCAR-8	Cytotoxic Assay	100 μM	48 h		GI50=0.1 μM	23871905
OVCAR5	Cytotoxic Assay	100 μM	48 h		GI50=0.41 μM	23871905
OVCAR4	Cytotoxic Assay	100 μM	48 h		GI50=0.37 μM	23871905
NCI-H522	Cytotoxic Assay	100 μM	48 h		GI50=0.03 μM	23871905
NCI-H322M	Cytotoxic Assay	100 μM	48 h		GI50=0.54 μM	23871905
NCI-H226	Cytotoxic Assay	100 μM	48 h		GI50=0.05 μM	23871905
MALME-3M	Cytotoxic Assay	100 μM	48 h		GI50=0.12 μM	23871905
M14	Cytotoxic Assay	100 μM	48 h		GI50=0.18 μM	23871905
LOXIMVI	Cytotoxic Assay	100 μM	48 h		GI50=0.07 μM	23871905
KM12	Cytotoxic Assay	100 μM	48 h		GI50=0.27 μM	23871905
IGROVI	Cytotoxic Assay	100 μM	48 h		GI50=0.17 μM	23871905
HOP92	Cytotoxic Assay	100 μM	48 h		GI50=0.1 μM	23871905
HCC2998	Cytotoxic Assay	100 μM	48 h		GI50=0.26 μM	23871905
EKVX	Cytotoxic Assay	100 μM	48 h		GI50=0.41 μM	23871905
Caki1	Cytotoxic Assay	100 μM	48 h		GI50=0.95 μM	23871905
ACHN	Cytotoxic Assay	100 μM	48 h		GI50=0.08 μM	23871905
786-0	Cytotoxic Assay	100 μM	48 h		GI50=0.13 μM	23871905
HOP62	Growth Inhibition Assay	100 μM	48 h		GI50=0.14 μM	23849207
Gurav	Growth Inhibition Assay	100 μM	48 h		GI50=0.17 μM	23849207
DWD	Growth Inhibition Assay	100 μM	48 h		GI50=0.1 μM	23849207
COLO205	Growth Inhibition Assay	100 μM	48 h		GI50=0.14 μM	23849207
LS180	Cytotoxic Assay		72 h	DMSO	IC50=0.04 μM	23827179
786	Cytotoxic Assay		48 h	DMSO	IC50=0.33 μM	23810282
HGF	Cytotoxic Assay	25 mM	24 h		IC50=0.5 μM	23802716
C643	Cytotoxic Assay	25 mM	24 h		IC50=0.07 μM	23802716
B16F10	Cytotoxic Assay		48 h	DMSO	IC50=0.072 μM	23777898
JURKAT-TALL	Cytotoxic Assay		48 h		IC50=0.0096 μM	23673218
U266	Cytotoxic Assay		72 h		IC50=0.67 μM	23600925
SW872	Cytotoxic Assay		72 h		IC50=0.14 μM	23600925
SK-UT-1	Cytotoxic Assay		72 h		IC50=0.076 μM	23600925
SK-ES-1	Cytotoxic Assay		72 h		IC50=0.042 μM	23600925
SK-BR-3	Cytotoxic Assay		72 h		IC50=0.09 μM	23600925
NCI-H929	Cytotoxic Assay		72 h		IC50=0.065 μM	23600925
MNNG-HOS	Cytotoxic Assay		72 h		IC50=0.59 μM	23600925
MDA-MB-436	Cytotoxic Assay		72 h		IC50=0.1 μM	23600925
A673	Apoptosis Assay		24 h	DMSO	Induction of apoptosis assessed as caspase-3 activation with EC50 of 0.39 μM	23600925
A673	Cytotoxic Assay		72 h		Cytotoxicity against human A673 cells after 72 hrs by resazurin/resorufin-based fluorescence assay with IC50 of 0.17 μM	23600925
MA9.3	Cytotoxic Assay	100 μM	48 h		IC50=0.086 μM	23578690
SK-MEL	Cytotoxic Assay		48 h	DMSO	IC50=1.5 μM	23547843
HeLa	Cytotoxic Assay	1 μM	72 h		IC50=0.01 μM	23470139
POS1	Growth Inhibition Assay		72 h		IC50=0.043 μM	23360521
OSRGA	Growth Inhibition Assay		72 h		IC50=0.093 μM	23360521
L929	Growth Inhibition Assay		72 h		IC50=0.161 μM	23360521
AT6-1	Growth Inhibition Assay		72 h		IC50=2 μM	23360521
WHCO1	Cytotoxic Assay		48 h	DMSO	IC50=0.5 μM	23353747
MOLT4	Cytotoxic Assay		48 h	DMSO	IC50=0.02 μM	23218718
MGC803	Growth Inhibition Assay	20 μM	72 h	DMSO	IC50=0.7 μM	23124210
Bcap37	Growth Inhibition Assay	20 μM	72 h	DMSO	IC50=1.28 μM	23124210
HL60/Vinc	Cytotoxic Assay	100 μg/mL	72 h	DMSO	Cytotoxicity against human HL60/Vinc cells with IC50 of 0.91 μM	23079523
BALB/3T3	Cytotoxic Assay	100 μg/mL	72 h	DMSO	IC50=1.91 μM	23079523
MG63	Cytotoxic Assay				IC50=2.7 μM	23043498
HL60/A	Cytotoxic Assay		18 h		IC50=6.7 μM	22985027
A549	Cytotoxic Assay				IC50=0.001 μM	22951040
MESSA/DX5	Growth Inhibition Assay	100 μg/mL	72 h	DMSO	IC50=0.25 μM	22944121
SKLU1	Cytotoxic Assay		48 h	DMSO	IC50=0.0067 μM	22858298
HCT15	Cytotoxic Assay		48 h	DMSO	IC50=0.005 μM	22858298
U2OS	Cytotoxic Assay	4 μM	72 h	DMSO	IC50=3.4 μM	22749279
B16			48 h		IC50=0.03 μM	22668451
MKN45	Cytotoxic Assay		48 h	DMSO	IC50=0.168 μM	22647723
HMEC1	Cytotoxic Assay	250 μg/mL	72 h		IC50=0.06 μM	22616554
BMDM	Cytotoxic Assay	250 μg/mL	72 h		IC50=4.6 μM	22616554
J774A.1	Cytotoxic Assay		72 h	DMSO	IC50=0.02 μM	22608675
WI38	Growth Inhibition Assay	100 μM	72 h	DMSO	IC50=0.04 μM	22555152
SW620	Growth Inhibition Assay	100 μM	72 h	DMSO	IC50=0.04 μM	22555152
NCI/ADR	Growth Inhibition Assay	25 μg/mL	48 h		IC50=13.5 μM	22537680
786-0	Growth Inhibition Assay	25 μg/mL	48 h		IC50=2.1 μM	22537680
SMMC7721	Cytotoxic Assay	500 μM	48 h	DMSO	IC50=0.37 μM	22537362
KB/VCR	Cytotoxic Assay	500 μM	48 h	DMSO	IC50=0.45 μM	22537362
U87MG	Growth Inhibition Assay	2 μM	48 h		IC50=0.79 μM	22515366
NCI-H290	Growth Inhibition Assay	2 μM	48 h		IC50=0.32 μM	22515366
LLC	Growth Inhibition Assay		48 h		IC50=0.207 μM	22515366
XF498	Cytotoxic Assay		48 h		IC50=0.015 μM	22483395
SK-MEL-2	Cytotoxic Assay		48 h		IC50=0.002 μM	22483395
K562/A02	Growth Inhibition Assay		72 h	DMSO	Antiproliferative activity against multidrug-resistant human K562/A02 cells overexpressing p-glycoprotein with IC50 of 46.69 μM	22450134
HuH7	Growth Inhibition Assay	25 ng/mL	72 h	DMSO	Antiproliferative activity assessed as inhibition of BrdU incorporation	22409771
HuH7	Function Assay	25 ng/mL	72 h	DMSO	Induction of senescence assessed as increase in senescence associated beta-galactosidase activity	22409771
SK-N-SH	Cytotoxic Assay		48 h		IC50=0.97 μM	22386528
SiHa	Growth Inhibition Assay				GI50=0.14 μM	22361684
B16	Cytotoxic Assay		72 h	DMSO	IC50=0.1 μM	22330634
SKOV3	Function Assay	5 μM	1 h	DMSO	Cellular uptake in doxorubicin-resistant human SKOV3 cells	22276998
SKOV3	Function Assay	5 μM	1 h	DMSO	Drug uptake in doxorubicin-resistant human SKOV3 cell nucleu	22276998
LNcaP	Apoptosis Assay	20 μg/mL	24 h	water	Induction of apoptosis assessed as increase in caspase 3/7 activation	22264758
PC3M	Cytotoxic Assay		72 h	DMSO	IC50=0.24 μM	22264149
QGY7701	Cytotoxic Assay		24 h	DMSO	IC50=8.65 μM	22182926
A375-C5	Growth Inhibition Assay		48 h	DMSO	GI50=0.0915 μM	22177409
RAW264.7	Cytotoxic Assay	10 μM	24 h	DMSO	Cytotoxicity assessed as cell viability	22129061
SKOV3	Growth Inhibition Assay	50 μM	72 h	DMSO	Antiproliferative activity against human SKOV3 cells overexpressing c-Src	22119472
BGC823	Cytotoxic Assay		96 h	DMSO	IC50=0.98 μM	22070654
HCT8	Cytotoxic Assay	5 μg/mL	72 h	DMSO	IC50=0.02 μM	22000949
MCF7	Function Assay	50 μg/mL	48 h	DMSO	Reduction in AP-2 gamma mRNA expression	22000946
MCF7	Function Assay	50 μg/mL	48 h	DMSO	Reduction in CYP19 mRNA expression	22000946
MCF7	Function Assay	50 μg/mL	48 h	DMSO	Reduction in VEGF mRNA expression	22000946
Neuro2a	Cytotoxic Assay		72 h		IC50=1.2 μM	21996519
PC3	Growth Inhibition Assay		48 h		GI50=0.09 μM	21875046
AGS	Growth Inhibition Assay		48 h		GI50=0.01 μM	21875046
BT20	Growth Inhibition Assay	10 μM	72 h		Antiproliferative activity	21852023
K562/A02	Cytotoxic Assay		48 h	DMSO	IC50=17.84 μM	21843940
Ca9-22	Cytotoxic Assay		72 h	DMSO	IC50=0.26 μM	21800859
SH-SY5Y	Cytotoxic Assay		48 h		IC50=0.5 μM	21797280
LAMA-84	Cytotoxic Assay		48 h		IC50=0.74 μM	21797280
A2780/ADR	Cytotoxic Assay				IC50=0.194 μM	21774499
A2780	Cytotoxic Assay				IC50=0.0012 μM	21774499
NCI-H187	Cytotoxic Assay		72 h		IC50=0.03 μM	21741833
KATO	Cytotoxic Assay		72 h		IC50=0.33 μM	21741833
ChaGo	Cytotoxic Assay		72 h		IC50=0.83 μM	21741833
BT474	Cytotoxic Assay		72 h		IC50=0.07 μM	21741833
HFF	Cytotoxic Assay		72 h		CC50=0.03 μM	21741131
Gurav	Cytotoxic Assay	100 μM	48 h	DMSO	GI50<0.1 μM	21737288
ZR-75B	Cytotoxic Assay		72 h		IC50=2.02 μM	21721528
ZR-75B	Cytotoxic Assay		72 h		IC50=0.04 μM	21721528
SW620	Cytotoxic Assay		72 h		IC50=0.01 μM	21721528
NIH3T3	Cytotoxic Assay		72 h		IC50=0.05 μM	21721528
NCI/ADR-RES	Cytotoxic Assay		72 h		IC50=9.3 μM	21721528
LLC-PK1	Cytotoxic Assay		72 h		IC50=0.2 μM	21721528
KBV1	Cytotoxic Assay		72 h		IC50=4.7 μM	21721528
KB3-1	Cytotoxic Assay		72 h		IC50=0.02 μM	21721528
HEK293	Cytotoxic Assay		72 h		IC50=0.42 μM	21721528
HEK293	Cytotoxic Assay		72 h		IC50=0.01 μM	21721528
Ew36	Cytotoxic Assay		72 h		IC50=0.01 μM	21721528
MG63	Growth Inhibition Assay		72 h		IC50=2.8 μM	21721519
PC3	Cytotoxic Assay	160 μg/mL	5 d	DMSO	GI50=0.021 μM	21711054
NCI-H23	Cytotoxic Assay	160 μg/mL	5 d	DMSO	GI50=0.005 μM	21711054
Hs578	Cytotoxic Assay	160 μg/mL	5 d	DMSO	GI50=0.006 μM	21711054
BT549	Cytotoxic Assay	160 μg/mL	5 d	DMSO	GI50=0.01 μM	21711054
HOP62	Cytotoxic Assay		24 h		GI50=0.14 μM	21676506
SW480	Growth Inhibition Assay		48 h		IC50=0.06 μM	21641694
S180	Growth Inhibition Assay		48 h		IC50=29.2 μM	21620529
H22	Growth Inhibition Assay		48 h		IC50=36.51 μM	21620529
P388D1	Growth Inhibition Assay				IC50=0.338 μM	21570750
KATOIII	Growth Inhibition Assay				IC50=4.38 μM	21570750
SK-MEL-28	Growth Inhibition Assay	25 nM	24 h	DMSO	IC50=0.6 μM	21553829
SF295	Growth Inhibition Assay	25 nM	24 h	DMSO	IC50=4.4 μM	21553829
A375	Growth Inhibition Assay		72 h	PBS	IC50=0.13 μM	21496972
HCT8	Cytotoxic Assay	0.58 μg/mL	69 h	DMSO	IC50=0.02 μM	21381705
NCI-N87	Growth Inhibition Assay		96 h	DMSO	IC50=0.4198 μM	21296467
HUVEC	Growth Inhibition Assay		96 h	DMSO	IC50=0.0303 μM	21296467
HepG2	Growth Inhibition Assay		96 h	DMSO	IC50=0.019 μM	21296467
CCRF	Growth Inhibition Assay		96 h	DMSO	IC50=0.0375 μM	21296467
IMR90	Cytotoxic Assay		48 h	DMSO	IC50=0.59 μM	21215623
Molt4/C8	Cytotoxic Assay		72 h	DMSO	IC50=0.2 μM	21144624
CEM	Cytotoxic Assay		72 h	DMSO	IC50=0.06 μM	21144624
MDA435	Cytotoxic Assay	10 μM	72 h	DMSO	IC50=0.0035 μM	21142180
MDA231	Cytotoxic Assay	10 μM	72 h	DMSO	IC50=4.7x10-5 μM	21142180
HL60R	Cytotoxic Assay	10 μM	72 h	DMSO	IC50=0.99 μM	21142180
C33A	Cytotoxic Assay	100 μg/mL	48 h		IC50=0.82 μM	21071221
MKN45	Growth Inhibition Assay		72 h		IC50=0.00019 μM	21067930
KB	Growth Inhibition Assay		72 h		IC50=0.00023 μM	21067930
H460	Growth Inhibition Assay		72 h		IC50=0.00038 μM	21067930
KB8.5	Cytotoxic Assay	50 μM	72 h	DMSO	IC50=3.3 μM	21035234
BJ	Cytotoxic Assay		48 h	DMSO	IC50=2.33 μM	20931970
ARPE19	Cytotoxic Assay		48 h	DMSO	IC50=0.76 μM	20931970
THP1	Cytotoxic Assay		72 h	DMSO	IC50=0.008 μM	20833057
SiHa	Cytotoxic Assay	4 μM	24 h		GI50=0.17 μM	20732817
DWD	Cytotoxic Assay	4 μM	24 h		GI50=0.1 μM	20732817
SKOV3	Cytotoxic Assay				IC50=0.001 μM	20719504
Hep3B	Cytotoxic Assay		11 d		IC50=0.14 μM	20718475
COLO205	Cytotoxic Assay		48 h	DMSO	GI50=0.1 μM	20673627
HCT-15	Cytotoxic Assay				IC50=0.038 μM	20594848
NUGC3	Cytotoxic Assay				GI50=0.202 μM	20579876
CCRF-CEM	Growth Inhibition Assay		48 h		GI50=0.02 μM	20570145
HAEC	Cytotoxic Assay	100 μM	24 h	DMSO	IC50=13 μM	20547797
PAXF1657L	Cytotoxic Assay	10 μM	24 h		IC50=0.052 μM	20545334
MEXF462NL	Cytotoxic Assay	10 μM	24 h		IC50=0.052 μM	20545334
MAXF401NL	Cytotoxic Assay	10 μM	24 h		IC50=0.052 μM	20545334
GXF251L	Cytotoxic Assay	10 μM	24 h		IC50=0.052 μM	20545334
CEM/ADR5000	Function Assay	1 μg/mL	6 h		Increase in ROS generation	20527917
CEM/ADR5000	Apoptosis Assay	1 μg/mL	24 h		Induction of apoptosis	20527917
PCO3	Cytotoxic Assay	250 μg/mL	48 h		GI50=0.17 μM	20413188
OVCAR-3	Cytotoxic Assay	250 μg/mL	48 h		GI50=0.07 μM	20413188
NCI/ADR	Cytotoxic Assay	250 μg/mL	48 h		GI50=5.99 μM	20413188
3T3	Cytotoxic Assay	10 μM	48 h		IC50=3.7 μM	20405844
HBI10A	Cytotoxic Assay				CC50<0.3 μM	20363140
U937	Cytotoxic Assay		72 h		IC50=0.00445 μM	20356655
NB-1	Cytotoxic Assay		72 h		IC50=0.00515 μM	20356655
K562	Cytotoxic Assay		72 h		IC50=0.00612 μM	20356655
HL60	Cytotoxic Assay		72 h		IC50=0.00113 μM	20356655
GOTO	Cytotoxic Assay		72 h		IC50=0.00473 μM	20356655
SK-N-MC	Cytotoxic Assay		72 h		IC50=0.12 μM	20303768
U87MG	Cytotoxic Assay		48 h		IC50=0.018 μM	20210346
SKHEP1	Cytotoxic Assay		48 h		IC50=0.01 μM	20210346
MIAPaCa2	Cytotoxic Assay	100 μM	72 h		IC50=0.01 μM	20207049
SKHep	Growth Inhibition Assay		48 h	DMSO	IC50=0.09 μM	20171108
MESSA/DX5	Function Assay	50 μM	2 h	water	Induction of DNA damage assessed as increase in comet-tail length	20151639
MESSA	Function Assay	25 μM	2 h	water	Cellular uptake	20151639
MESSA	Function Assay	50 μM	2 h	water	Induction of DNA damage assessed as increase in comet-tail length	20151639
MCF10A	Cytotoxic Assay	40 μM	48 h	DMSO	IC50=0.18 μM	20064676
IMR32	Growth Inhibition Assay		48 h	DMSO	IC50=1.7 μM	19939522
MOLT3	Cytotoxic Assay				IC50=0.04 μM	19824618
HuCCa1	Cytotoxic Assay		4 d		IC50=0.64 μM	19824618
HCT15	Cytotoxic Assay				IC50=0.037 μM	19819696
MDA-MB-453	Cytotoxic Assay	0.58 μg/mL	69 h	DMSO	IC50=0.12 μM	19788290
HL60/MX2	Cytotoxic Assay		48 h	DMSO	IC50=8.046 μM	19743858
2008/P	Cytotoxic Assay		5 d	DMSO	IC50=0.063 μM	19725578
2008/MRP1	Cytotoxic Assay		5 d	DMSO	IC50=0.577 μM	19725578
Colon 26-L5	Cytotoxic Assay		72 h	DMSO	IC50=3.12 μM	19689125
B16-BL6	Cytotoxic Assay		72 h	DMSO	IC50=3.07 μM	19689125
KB	Cytotoxic Assay		72 h	DMSO	IC50=0.001 μM	19655762
HCT116	Cytotoxic Assay		72 h	DMSO	IC50=0.006 μM	19655762
U251	Growth Inhibition Assay		72 h	DMSO	IC50=0.6 μM	19632833
OS-RC2	Growth Inhibition Assay		72 h	DMSO	IC50=5.4 μM	19632833
MOLT4	Growth Inhibition Assay		72 h	DMSO	IC50=0.002 μM	19632833
L78	Growth Inhibition Assay		72 h	DMSO	IC50=2.4 μM	19632833
IMR90	Growth Inhibition Assay		72 h	DMSO	IC50=1 μM	19632833
SK-N-SH	Apoptosis Assay	5 μM			Induction of apoptosis assessed as caspase 3 activity	19631549
SK-N-SH	Apoptosis Assay	5 μM			Induction of apoptosis at 0.5 to 5 uM	19631549
SNU1	Cytotoxic Assay				IC50=4 μM	19585998
MDA-MB-231	Growth Inhibition Assay	200 μM	48 h	DMSO	Antitumor activity against human estrogen receptor deficient MDA-MB-231 cells with GI50 of 0.01086 μM	19428155
U251	Function Assay	1 μM	16 h	DMSO	Inhibition of HIF1 activation in human U251 cells stably transfected in pGL2-TK-HRE plasmid with EC50 of 0.12 μM	19405508
SKOV3	Growth Inhibition Assay				GI50=0.021 μM	19394218
MESSA	Growth Inhibition Assay				GI50=0.0051 μM	19394218
A549	Growth Inhibition Assay				GI50=0.003 μM	19394218
A431	Growth Inhibition Assay				GI50=0.0012 μM	19394218
PA1	Cytotoxic Assay	10 μM	72 h		IC50=0.63 μM	19361894
HBL100	Cytotoxic Assay	10 μM	72 h		IC50=0.24 μM	19361894
ECV304	Cytotoxic Assay	10 μM	72 h		Cytotoxicity	19361894
WTK1	Function Assay	0.1 μM	72 h		Cell cycle arrest in human WTK1 cells expressing p53 M237I mutant assessed as accumulation at sub-G0/G1 phase	19349174
WTK1	Function Assay	25 μM	8 h		Induction of p53 transcriptional activity in human WTK1 cells expressing p53 M237I mutant assessed as p21-Cip1/Waf1 expression	19349174
WTK1	Function Assay	25 μM	8 h		Induction of p53 phosphorylation at Ser15 in human WTK1 cells expressing p53 M237I mutant	19349174
WTK1	Function Assay	0.1 μM	8 h		Induction of p53 transcriptional activity in human WTK1 cells expressing p53 M237I mutant assessed as p21-Cip1/Waf1 expression	19349174
WTK1	Apoptosis Assay	0.1 μM	8 h		Induction of apoptosis in human WTK1 cells expressing p53 M237I mutant assessed as increase in PARP cleavage	19349174
WTK1	Apoptosis Assay	0.1 μM	8 h		Induction of apoptosis in human WTK1 cells expressing p53 M237I mutant assessed as decrease in pro-caspase 3 level	19349174
WTK1	Apoptosis Assay	0.1 μM	8 h		Induction of apoptosis in human WTK1 cells expressing p53 M237I mutant assessed as increase in cleaved caspase 3 level	19349174
WTK1	Function Assay	0.1 μM	8 h		Induction of p53 phosphorylation at Ser15 in human WTK1 cells expressing p53 M237I mutant	19349174
TK6	Function Assay	25 μM	8 h		Induction of p53 transcriptional activity in human TK6 cells expressing wild type p53 assessed as p21-Cip1/Waf1 expression	19349174
TK6	Function Assay	25 μM	8 h		Induction of p53 phosphorylation at Ser15 in human TK6 cells expressing wild type p53	19349174
TK6	Function Assay	0.1 μM	72 h		Cell cycle arrest in human TK6 cells expressing wild type p53 assessed as accumulation at sub-G0/G1 phase	19349174
TK6	Apoptosis Assay	0.1 μM	8 h		Induction of apoptosis in human TK6 cells expressing wild type p53 assessed as increase in PARP cleavage	19349174
TK6	Apoptosis Assay	0.1 μM	8 h		Induction of apoptosis in human TK6 cells expressing wild type p53 assessed as decrease in pro-caspase 3 level	19349174
TK6	Apoptosis Assay	0.1 μM	8 h		Induction of apoptosis in human TK6 cells expressing wild type p53 assessed as increase in cleaved caspase 3 level	19349174
TK6	Function Assay	0.1 μM	8 h		Induction of p53 transcriptional activity in human TK6 cells expressing wild type p53 assessed as p21-Cip1/Waf1 expression	19349174
TK6	Function Assay	0.1 μM	8 h		Induction of p53 phosphorylation at Ser15 in human TK6 cells expressing wild type p53	19349174
NH32	Growth Inhibition Assay	0.1 μM	72 h		Cell cycle arrest in p53 deficient human NH32 cells assessed as accumulation at sub-G0/G1 phase	19349174
NH32	Apoptosis Assay	0.1 μM	8 h		Induction of apoptosis in p53 deficient human NH32 cells assessed as increase in PARP cleavage	19349174
NH32	Apoptosis Assay	0.1 μM	8 h		Induction of apoptosis in p53 deficient human NH32 cells assessed as decrease in pro-caspase 3 level	19349174
NH32	Apoptosis Assay	0.1 μM	8 h		Induction of apoptosis in p53 deficient human NH32 cells assessed as increase in cleaved caspase 3 level	19349174
SMMC-7541	Cytotoxic Assay		72 h		IC50=0.0037 μM	19285863
T47D	Apoptosis Assay	10 μM	48 h	DMSO	Induction of apoptosis assessed as caspase activation with EC50 of 0.57 μM	19282188
SNU398	Apoptosis Assay	10 μM	48 h	DMSO	Induction of apoptosis assessed as caspase activation with EC50 of 0.39 μM	19282188
MEF	Cytotoxic Assay		72 h		IC50=3.4 μM	19186946
Hs888Lu	Cytotoxic Assay		72 h		IC50=1.4 μM	19186946
HL60	Cytotoxic Assay	100 μM	72 h	DMSO	IC50=0.018 μM	19136263
A2780/DX3	Function Assay	10 μM	2 h	saline	Reversal of P-gp 170-mediated multidrug resistance assessed as increase in intracellular doxorubicin accumulation	19093883
XF498	Growth Inhibition Assay		96 h	DMSO	IC50=0.16 μM	19053767
MT4	Growth Inhibition Assay		96 h	DMSO	IC50=0.003 μM	19053767
HT-29	Growth Inhibition Assay		96 h	DMSO	IC50=0.002 μM	19053767
HT-29	Cytotoxic Assay		72 h	DMSO	IC50=0.018 μM	19028425
Bel7402	Cytotoxic Assay		72 h	DMSO	IC50=0.021 μM	19028425
DU145	Cytotoxic Assay	25 μg/mL	72 h		IC50=0.0039 μM	18829316
MRC5	Cytotoxic Assay		72 h		IC50=0.1 μM	18783950
Jurkat	Cytotoxic Assay		72 h		IC50=0.03 μM	18783950
AW8507	Cytotoxic Assay	100 μM	48 h		GI50=0.17 μM	18657979
AW13516	Cytotoxic Assay	100 μM	48 h		GI50=0.1 μM	18657979
SK-MEL-2	Growth Inhibition Assay		72 h		GI50=0.11 μM	18585035
L1210	Function Assay	0.5 μg/mL	12 h	water	Cell cycle arrest in mouse L1210 cells by accumulation at G2/M phase	18508273
LLC	Cytotoxic Assay		72 h	DMSO	IC50=0.01 μM	18440233
Colon 26-L5	Cytotoxic Assay		72 h	DMSO	IC50=0.01 μM	18440233
MCF7/DX	Cytotoxic Assay		24 h	water	Cytotoxicity against human doxorubicin-resistant MCF7/Dx cells with IC50 of 5.3 μM	18429610
Caco2	Function Assay	50 nM	48 h	water	Decrease in cyclin A expression	18429610
SNU638	Cytotoxic Assay		72 h	DMSO	IC50=0.052 μM	18321715
HT1080	Cytotoxic Assay		72 h	DMSO	IC50=0.022 μM	18321715
Hep2	Cytotoxic Assay		24 h	DMSO	IC50=0.42 μM	18281125
A375	Cytotoxic Assay		24 h	DMSO	IC50=0.01 μM	18281125
K/VP.5	Growth Inhibition Assay		72 h	DMSO	Growth inhibition of human K/VP.5 cells with IC50 of 0.41 μM	18258442
PC3M	Growth Inhibition Assay		72 h	DMSO	IC50=1.11 μM	18247573
SJSA1	Cytotoxic Assay	1 μM	6 h		Cytotoxicity against human SJSA1 cells expressing GRP78 with IC50 of 0.0062 μM	18243696
HepG2	Cytotoxic Assay	100 μM	72 h		IC50=0.07 μM	18181575
U373MG	Growth Inhibition Assay		24 h	DMSO	Growth inhibition of human U373MG cells containing UhiCE expressing plasmid with IC50 of 0.19953 μM	18173233
U373MG	Growth Inhibition Assay		24 h	DMSO	Growth inhibition of human U373MG cells containing UhCE1 expressing plasmid with IC50 of 0.15849 μM	18173233
U373MG	Growth Inhibition Assay		24 h	DMSO	Growth inhibition of human U373MG cells containing U373IRES plasmid with IC50 of 0.15849 μM	18173233
SNU638	Growth Inhibition Assay		72 h		IC50=0.07 μM	18063366
SF268	Growth Inhibition Assay		72 h	DMSO	IC50=0.04 μM	18052326
W301	Growth Inhibition Assay	50 mM	48 h	DMSO	Growth inhibition of Saccharomyces cerevisiae W301 with IC50 of 10 μM	18040043
Daudi	Cytotoxic Assay	100 μM	96 h		Cytotoxicity against human Daudi cells under deem light with IC50 of 0.3 μM	17993275
2780.9S	Cytotoxic Assay	100 μM	96 h		Cytotoxicity against human 2780.9S cells under deem light with IC50 of 15 μM	17993275
MCF7	Growth Inhibition Assay	100 μM	72 h	DMSO	IC50=0.0007 μM	17949859
KB3.1	Growth Inhibition Assay	100 μM	72 h	DMSO	IC50=0.0004 μM	17949859
WI38	Growth Inhibition Assay	100 μM	72 h		IC50=0.1 μM	17935309
MIAPaCa2	Growth Inhibition Assay	100 μM	72 h		IC50=0.024 μM	17935309
UACC257	Growth Inhibition Assay		48 h		IC50=0.20893 μM	17696516
RXF393	Growth Inhibition Assay		48 h		IC50=0.17783 μM	17696516
IGROVI	Growth Inhibition Assay		48 h		IC50=0.10965 μM	17696516
BxPc3	Growth Inhibition Assay		3 h		IC50=0.31623 μM	17696516
PC3	Function Assay	20 nM	72 h		Induction of senescence in human PC3 cells	17658777
UO31	Cytotoxic Assay		72 h		IC50=0.1 μM	17656091
DLD1	Cytotoxic Assay		72 h		IC50=0.1 μM	17656091
UACC62	Growth Inhibition Assay		48 h	DMSO	GI50=0.094 μM	17614292
LNcaP	Cytotoxic Assay			DMSO	ED50=0.04 μM	17591444
1A9	Cytotoxic Assay			DMSO	ED50=0.02 μM	17591444
ZR-751	Cytotoxic Assay		72 h	DMSO	IC50=0.04 μM	17585747
BGC823	Cytotoxic Assay		48 h		IC50=0.35 μM	17583953
2780AD	Growth Inhibition Assay	10 μM			Growth inhibition of multidrug-resistant human 2780AD cells with IC50 of 0.298 μM	17490878
KB-VIN	Cytotoxic Assay		72 h	DMSO	Cytotoxicity against human multidrug-resistant KB-VIN cells with EC50 of 1.7 μM	17378530
MeVo	Cytotoxic Assay		48 h	water	IC50=0.82 μM	17375902
CEM/VM1	Cytotoxic Assay		72 h	DMSO	Cytotoxicity against teniposide-resistant CEM/VM1 cells with IC50 of 0.55 μM	17323939
CEM/VLB100	Cytotoxic Assay		72 h	DMSO	Cytotoxicity against CEM/VLB100 cells with IC50 of 3.71 μM	17323939
K562I/S9	Growth Inhibition Assay		72 h	DMSO	Antiproliferative activity against human multidrug-resistant K562i/S9 cells expressing Pgp with IC50 of 2 μM	17276690
2780AD	Cytotoxic Assay	10 μM			Cytotoxicity againt multidrug-resistant human 2780AD cells with IC50 of 0.909 μM	17239597
TRAMP-C2H	Growth Inhibition Assay		72 h	water	GI50=0.0158 μM	17181166
TRAMP-C1A	Growth Inhibition Assay		72 h	water	GI50=0.0204 μM	17181166
K562	Growth Inhibition Assay		48 h	DMSO/methanol	IC50=0.01 μM	17181164
MDA435/LCC6	Growth Inhibition Assay		4 d	DMSO	IC50=0.3 μM	17154505
MDA-MB-231	Cytotoxic Assay		72 h	DMSO	IC50=0.03 μM	17141923
A2780	Growth Inhibition Assay	1 μM	4 d	DMSO	Inhibition of doxorubicin-resistant A2780 cell growth with IC50 of 0.061 μM	17125254
A2780	Growth Inhibition Assay	1 μM	4 d	DMSO	Inhibition of A2780 cell growth with IC50 of 0.003 μM	17125254
Vero	Cytotoxic Assay		24 h	DMSO	IC50=0.50119 μM	17125253
SK-HEP-1	Growth Inhibition Assay		24 h	DMSO	IC50=0.05012 μM	17125253
SHP77	Growth Inhibition Assay		24 h	DMSO	IC50=0.12589 μM	17125253
H9c2	Growth Inhibition Assay		24 h	DMSO	IC50=0.01995 μM	17125253
AGS	Cytotoxic Assay				IC50=0.31 μM	17125240
HA22T	Cytotoxic Assay		72 h	DMSO	IC50=0.31 μM	17107806
HL60/ADR	Cytotoxic Assay	1 mM	72 h	DMSO	Cytotoxicity against adriamycin-resistant HL60/ADR cells expressing MRP1 with IC50 of 2 μM	17069934
BC	Cytotoxic Assay				IC50=0.288 μM	16933890
Wil2-NS	Growth Inhibition Assay		96 h	DMSO	IC50=0.02 μM	16913700
G361	Growth Inhibition Assay		96 h	DMSO	IC50=0.09 μM	16913700
C8166	Growth Inhibition Assay		96 h	DMSO	IC50=0.02 μM	16913700
ACHN	Growth Inhibition Assay		96 h	DMSO	IC50=0.001 μM	16913700
5637	Growth Inhibition Assay		96 h	DMSO	IC50=0.02 μM	16913700
MCF7	Function Assay	0.5 μg/mL	24 h	DMSO	Inhibition of p53-mediated transactivation	16862141
VA13	Growth Inhibition Assay		48 h	DMSO	IC50=0.22 μM	16499322
MESSA/DX5	Cytotoxic Assay				Cytotoxicity against multidrug-resistant MES-SA/Dx5 cells with IC50 of 1.56 μM	16169719
MES-SA	Cytotoxic Assay				IC50=0.016 μM	16169719
HSG	Cytotoxic Assay		24 h		CC50=1.9 μM	15745812
HSC-2	Cytotoxic Assay		24 h		CC50=0.68 μM	15745812
NCI-H69	Cytotoxic Assay		6 d		IC50=0.0113 μM	15743178
VA13	Cytotoxic Assay		48 h	DMSO	IC50=0.38 μM	15730243
MeVo	Growth Inhibition Assay		24 h	water	IC50=0.82 μM	15715481
WI38	Cytotoxic Assay		48 h	DMSO	IC50=0.3 μM	15620238
Rtx6	Growth Inhibition Assay		4 h	DMSO	Growth inhibition of ER expressing Rtx6 cells	15588086
MEL-28	Cytotoxic Assay				IC50=0.03 μM	15225700
Col2	Cytotoxic Assay		72 h	DMSO	IC50=0.52 μM	15177438
U937/GTB	Cytotoxic Assay		72 h		IC50=0.1 μM	14987049
RPMI8226	Cytotoxic Assay		72 h		IC50=0.1 μM	14987049
T47D	Cytotoxic Assay		72 h		IC50=0.007 μM	14980672
MD-MB468	Cytotoxic Assay		72 h		IC50=0.007 μM	14980672
TRAMP-C2G	Growth Inhibition Assay		72 h		IC50=0.0287 μM	14971910
TRAMP-C2D	Growth Inhibition Assay		72 h		IC50=0.0465 μM	14971910
MDA-MB-435	Growth Inhibition Assay		4 h	DMSO	IC50=0.15 μM	14971899
PANC-1	Growth Inhibition Assay		72 h	DMSO	GI50=3.5 μM	14971893
MEL-28	Growth Inhibition Assay		72 h	DMSO	GI50=3.1 μM	14971893
LOVO	Growth Inhibition Assay		72 h	DMSO	GI50=0.01 μM	14971893
LNcaP	Growth Inhibition Assay		72 h	DMSO	GI50=0.02 μM	14971893
IGROV	Growth Inhibition Assay		72 h	DMSO	GI50=4.4 μM	14971893
HeLa	Growth Inhibition Assay		72 h	DMSO	GI50=0.01 μM	14971893
DU145	Growth Inhibition Assay		72 h	DMSO	GI50=0.02 μM	14971893
DuPRO	Cytotoxic Assay				EC50=1.8 μM	14667208
SF268	Cytotoxic Assay				GI50=8.5 × 10-7 μg/mL	14640532
MCF7	Cytotoxic Assay				GI50=8.0 × 10-7 μg/mL	14640532
H460	Cytotoxic Assay				GI50=3.0 × 10-7 μg/mL	14640532
HT1080	Growth Inhibition Assay		72 h	DMSO	IC50=0.059 μM	14640513
Neuro2A	Function Assay	3 ug/mL	24 h		Neuritogenic activity assessed as morphological changes	14575440
RXF944L	Growth Inhibition Assay		72 h	DMSO	IC50=0.06 μM	12852768
LXF629L	Growth Inhibition Assay		72 h	DMSO	IC50=0.07 μM	12852768
LXF529L	Growth Inhibition Assay		72 h	DMSO	IC50=0.03 μM	12852768
401NL	Growth Inhibition Assay		72 h	DMSO	IC50=0.01 μM	12852768
L2987	Cytotoxic Assay				IC50=0.3 μM	12798317
P388	Growth Inhibition Assay		72 h		IC50=0.015 μM	12620081
Jurkat	Growth Inhibition Assay		72 h		IC50=0.0096 μM	12620081
8226/S	Cytotoxic Assay		72 h	DMSO	Cytotoxity against 8226/S	12570378
8226/DOX1V	Cytotoxic Assay		72 h	DMSO	Cytotoxity against 8226/DOX1V	12570378
LXFL529	Growth Inhibition Assay		36 h		IC50=0.04 μM	12459020
L1210	Cytotoxic Assay		48 h	DMSO	IC50=0.025 μM	12443763
HET-SR-2SC-LNM	Growth Inhibition Assay		72 h	deionized water	IC50=0.052 μM	12431060
2SC/20	Growth Inhibition Assay		72 h	deionized water	IC50=0.62 μM	12431060
Wil-NS	Growth Inhibition Assay		96 h	DMSO	IC50=0.02 μM	12431049
Raji	Growth Inhibition Assay		96 h	DMSO	IC50=0.03 μM	12431049
Hep-2	Growth Inhibition Assay		96 h	DMSO	IC50=0.04 μM	12431049
SK-MES-1	Cytotoxic Assay		6 d	DMSO	IC50=0.03 μM	12431048
SKMEL-28	Cytotoxic Assay		6 d	DMSO	IC50=0.06 μM	12431048
CRL7065	Cytotoxic Assay		6 d	DMSO	IC50=0.5 μM	12431048
UMUC3	Cytotoxic Assay				IC50=0.0092 μM	12392727
U251	Cytotoxic Assay				IC50=0.09 μM	12039588
H69P	Growth Inhibition Assay		4 d		IC50=0.027 μM	11806725
H69/LX4	Growth Inhibition Assay		4 d		Inhibitory activity against drug resistant H69/LX4 cell overexpressing P-glycoprotein with IC50 of 0.137 μM	11806725
H69P	Cytotoxic Assay		5-6 d		IC50=0.0273 μM	11806724
COR-L23	Cytotoxic Assay		5-6 d		IC50=0.0201 μM	11806724
HCT116	Growth Inhibition Assay				GI50=0.023 μM	11755363
SKVLB1	Cytotoxic Assay		72 h	DMSO	Cytotoxicity against human multidrug-resistant SKVLB1 cell with IC50 of 2.2 μM	11754602
UACC62	Cytotoxic Assay		3-5 m	DMSO	GI50=0.094 μM	11678649
U87	Cytotoxic Assay	10 μM		DMSO	Cytotoxicity against U87 resistant cell with IC50 of 0.14 μM	11606141
U87	Cytotoxic Assay	10 μM		DMSO	Cytotoxicity against U87 sensitive cell with IC50 of 0.06 μM	11606141
U373	Cytotoxic Assay	10 μM		DMSO	Cytotoxicity against U373 sensitive cell with IC50 of 0.01 μM	11606141
T24	Cytotoxic Assay	10 μM		DMSO	Cytotoxicity against T24 sensitive cell with IC50 of 0.02 μM	11606141
LoVo	Cytotoxic Assay	10 μM		DMSO	Cytotoxicity against LoVo sensitive cell with IC50 of 0.008 μM	11606141
J82	Cytotoxic Assay	10 μM		DMSO	Cytotoxicity against J82 sensitive cell with IC50 of 0.03 μM	11606141
CNS	Growth Inhibition Assay		48 h		GI50=0.064 μM	11473418
RENCA	Growth Inhibition Assay		48 h		IC50<0.01 μM	11454467
CH1	Cytotoxic Assay		96 h	DMSO	IC50=0.0063 μM	11123989
UA375	Cytotoxic Assay		24 h	DMSO	IC50=0.112 μM	10956214
OVCAR-3	Cytotoxic Assay		24 h	DMSO	IC50=0.035 μM	10956214
D40	Cytotoxic Assay		24 h	DMSO	IC50=1.172 μM	10956214
L1210	Cytotoxic Assay				IC50=0.025 μM	10890167
ZR-75-1	Cytotoxic Assay	100 μM	48 h	Saline	Cytotoxicity against ZR-75-1 cell (ER+,pgr+,mutant p53) with IC50 of 0.009 μM	10780913
T47D	Cytotoxic Assay	100 μM	48 h	Saline	Cytotoxicity against T47D cell (ER+,mutant p53) with IC50 of 0.007 μM	10780913
SKBR-3	Cytotoxic Assay	100 μM	48 h	Saline	Cytotoxicity against SKBR-3 cell (ER-amplified erB2,mutant p53) with IC50 of 0.02 μM	10780913
NCI-H647	Cytotoxic Assay	100 μM	48 h	Saline	Cytotoxicity against NCI-H647 cell (mutant p53) with IC50 of 0.001 μM	10780913
NCI-H358	Cytotoxic Assay	100 μM	48 h	Saline	Cytotoxicity against NCI-H358 cell (mutant p53) with IC50 of 0.13 μM	10780913
NCI-H322	Cytotoxic Assay	100 μM	48 h	Saline	Cytotoxicity against NCI-H322 cell (mutant p53) with IC50 of 0.03 μM	10780913
NCI-H226	Cytotoxic Assay	100 μM	48 h	Saline	Cytotoxicity against NCI-H226 cell (mutant p53) with IC50 of 0.008 μM	10780913
MDA-468	Cytotoxic Assay	100 μM	48 h	Saline	Cytotoxicity against MDA-468 cell (ER-, amplified EGFR, mutant p53) with IC50 of 0.005 μM	10780913
MDA-231	Cytotoxic Assay	100 μM	48 h	Saline	Cytotoxicity against MDA-231 cell (ER-,EGFR+,mutant p53) with IC50 of 0.006 μM	10780913
H460pv8	Cytotoxic Assay	100 μM	48 h	Saline	IC50=0.004 μM	10780913
H460pv8/eto	Cytotoxic Assay	100 μM	48 h	Saline	IC50=0.02 μM	10780913
U937	Function Assay		24 h	DMSO	Inhibits incorporation of [3H]thymidine with IC50 of 0.1 μM	10780911
T24	Growth Inhibition Assay				IC50=0.64 μM	10698436
PC14	Growth Inhibition Assay				IC50=1.9 μM	10698436
MKN-1	Growth Inhibition Assay				IC50=0.64 μM	10698436
HMV-1	Growth Inhibition Assay				IC50=0.28 μM	10698436
P388	Cytotoxic Assay		72 h		GI50=0.012 μM	10691710
3Y1	Cytotoxic Assay		72 h		GI50=0.0038 μM	10691710
PACA-2	Cytotoxic Assay		6 d		ED50=0.0063 μM	10498214
K562R	Cytotoxic Assay		72 h		IC50=0.0156 μM	10479282
DU145	Cytotoxic Assay		72 h		IC50=0.0528 μM	10479282
MEL-AC	Cytotoxic Assay	100 μM			IC50=1.2 μM	10476861
HTB-54	Cytotoxic Assay	100 μM			IC50=1.1 μM	10476861
KB	Growth Inhibition Assay		4 d	DMSO	Antiproliferative activity against drug-resistant KBV20C with IC50 of 0.4 μM	10411476
KB	Growth Inhibition Assay		4 d	DMSO	Antiproliferative activity against drug-resistant KBMDR with IC50 of 0.5 μM	10411476
KB	Growth Inhibition Assay		4 d	DMSO	Antiproliferative activity against drug-resistant KBCampwith IC50 of 0.06 μM	10411476
KB	Growth Inhibition Assay		4 d	DMSO	Antiproliferative activity against drug-resistant KB7D with IC50 of 0.65 μM	10411476
CHO-K1	Growth Inhibition Assay		4 d	DMSO	IC50=0.4 μM	10411476
CCRF-SB	Growth Inhibition Assay		4 d	DMSO	IC50=0.01 μM	10411476
MDA-MB-361	Cytotoxic Assay		1 h	DMSO	Cytotoxicity against MDA MB 361 expressing carboxypeptidase stCPG2 with IC50 of 0.018 μM	10395490
MDA-MB-361	Cytotoxic Assay		1 h	DMSO	Cytotoxicity against MDA-MB-361 expressing LacZ with IC50 of 0.012 μM	10395490
SF-295	Cytotoxic Assay		72 h		IC50=0.035 μM	9873661
HOP62	Cytotoxic Assay		72 h		IC50=0.004 μM	9873661
PC14	Cytotoxic Assay		4 d	DMSO	IC50=0.29 μM	9822542
Panc02	Growth Inhibition Assay	10 μM	6 d		IC50=0.065 μM	9526570
CHO	Growth Inhibition Assay	10 μM	6 d		IC50=0.06 μM	9526570
CA755	Growth Inhibition Assay	10 μM	6 d		IC50=0.014 μM	9526570
MCF7/VP16	Cytotoxic Assay	50 μg/mL	72 h		IC50=14.2 μM	9022792
SKVLB	Cytotoxic Assay		72 h		IC50=6.54 μM	9003520
C6	Cytotoxic Assay	2.5 mg/mL	5 h	DMSO	Cytotoxicity assessed as cell release	8988604
C6	Function Assay	2.5 mg/mL	4 h	DMSO	Inhibition of tubulin polymerization	8988604
C6	Cytotoxic Assay	2.5 mg/mL	72 h	DMSO	Cytotoxicity against rat C6 cells at 50 ug/mL to 2.5 mg/mL	8988604
MCF	Function Assay				Reversal of P-gp-mediated multidrug resistance assessed as enhancement in cytotoxicity of adriamycin	8984151
WiDr	Cytotoxic Assay				Activity against sensitive WiDr cell with IC50 of 0.053 μM	8960558
MXF7	Cytotoxic Assay				Antitumor activity against doxorubicin-resistant MXF7 with IC50 of 1.172 μM	8960558
MXF7	Cytotoxic Assay				Antitumor activity against MXF7 with IC50 of 0.028 μM	8960558
MXF7	Cytotoxic Assay				Antitumor activity against sensitive MXF7 with IC50 of 0.028 μM	8960558
A2780-DX5	Cytotoxic Assay				Cytotoxicity against doxorubicin-resistant A2780-DX5 with IC50 of 0.357 μM	8831755
A2780-CP3	Cytotoxic Assay				Cytotoxicity against A2780-CP3 with IC50 of 0.056 μM	8831755
A2780-C25	Cytotoxic Assay				Cytotoxicity against A2780-C25 with IC50 of 0.063 μM	8831755
AUC375	Growth Inhibition Assay				IC50=0.112 μM	8648600
SKVLB	Cytotoxic Assay				IC50=5.6 μM	8576914
EAC	Cytotoxic Assay		72 h		IC50=0.35 μM	8350087
C38	Cytotoxic Assay				IC50=0.01 μM	8057291
LOX	Cytotoxic Assay				IC50=0.042 μM	7853331
H2981	Cytotoxic Assay				IC50=0.5 μM	7731023
EMT6	Growth Inhibition Assay	0.5 μM	1 h		Inhibition of proliferation by measuring for surviving fractions with ED50 of 0.0001 μM	7452674
HT-29	Growth Inhibition Assay	0.01 μg	48 h		Antiproliferative activity	2778449
KBM-3	Growth Inhibition Assay				IC50=0.026 μM	1995877
MLM	Cytotoxic Assay				ED50=0.13 μM	1875350
SKMEL-5	Growth Inhibition Assay				ED50=3.2 μM	1613753
T-MT4	Growth Inhibition Assay				ID50=0.01 μM	1548681
T-H9	Growth Inhibition Assay				ID50=0.01 μM	1548681
T-C8166	Growth Inhibition Assay				ID50=0.01 μM	1548681
H9	Growth Inhibition Assay				Inhibition of H9 cells chronically infected with the HIV-1 (H9/IIIB) with ID50 of 0.01 μM	1548681
N417	Growth Inhibition Assay				IC50=0.23 μM	1479585
H69	Growth Inhibition Assay				IC50=0.87 μM	1479585
MCF7/ADR	Growth Inhibition Assay				IC50=3.1 μM	1447730
8226/ADR	Growth Inhibition Assay				IC50=0.56 μM	1447730
8226	Growth Inhibition Assay				IC50=0.033 μM	1447730
CHO	Growth Inhibition Assay				IC50=0.06 μM	1354750
Yoshida sarcoma	Function Assay				Inhibition of [3H]TdR incorporation with IC50 of 0.02 μM	1322986
L1210	Function Assay				Inhibition of RNA synthesis with ED50 of 0.58 μM	490536
A431	Cytotoxic Assay				IC50=0.00135 μM	24890652
HT-29	Function Assay	25 μM	24 h		Inhibition of MRP3-mediated drug resistance in doxorubicin-resistant human HT-29 cells assessed as increase in drug accumulation	24900337
HT-29	Function Assay	5 μM	24 h		Inhibition of MRP3 in doxorubicin-resistant human HT-29 cells assessed as nitration of tyrosine residues	24900337
HT-29	Function Assay	5 μM	1 h		Inhibition of MRP3-mediated drug resistance in doxorubicin-resistant human HT-29 cells assessed as increase in drug accumulation	24900337
HT-29	Function Assay	5 μM	1 h		Induction of nitrite production in doxorubicin-resistant human HT-29 cells	24900337
NAR	Function Assay	5 μM	2 h		Drug uptake in doxorubicin-resistant human NAR cell plasma membrane	24900344
NAR	Cytotoxic Assay	10 μM	24 h		Cytotoxicity assessed as induction of cell death	24900344
NAR	Cytotoxic Assay	100 μM	24 h		Cytotoxicity against doxorubicin-resistant human NAR cells assessed as cell viability	24900344
DA-3	Cytotoxic Assay	60 μM	24 h		IC50=4 μM	24900668
DA-3	Function Assay	10 μM	10 h		Cellular uptake in mouse DA-3 cell mitochondria	24900668
DA-3	Function Assay	10 μM	10 h		Cellular uptake in mouse DA-3 cell lysosome	24900668
DA-3	Function Assay	10 μM	10 h		Cellular uptake in mouse DA-3 cell nucleus	24900668
YOYO1	Function Assay				Displacement of YOYO1 from phage lambda DNA with IC50 of 0.6 μM	24900668
KCL22	Growth Inhibition Assay		48 h		IC50=1.8 μM	24941129
Saos2	Growth Inhibition Assay	40 μM	24 h	DMSO	IC50=0.33 μM	24941130
IMR32	Cytotoxic Assay	250 μM	48 h	DMSO	IC50=0.0051 μM	24996140
NTERA-S-cl-D1	Growth Inhibition Assay				IC50=1.37 nM	SANGER
EW-16	Growth Inhibition Assay				IC50=1.91 nM	SANGER
HUTU-80	Growth Inhibition Assay				IC50=2.12 nM	SANGER
KS-1	Growth Inhibition Assay				IC50=3.44 nM	SANGER
HT-144	Growth Inhibition Assay				IC50=3.51 nM	SANGER
MFH-ino	Growth Inhibition Assay				IC50=3.99 nM	SANGER
LC-2	Growth Inhibition Assay				IC50=5.07 nM	SANGER
SW982	Growth Inhibition Assay				IC50=6.05 nM	SANGER
ES4	Growth Inhibition Assay				IC50=6.11 nM	SANGER
KGN	Growth Inhibition Assay				IC50=6.34 nM	SANGER
RH-1	Growth Inhibition Assay				IC50=6.73 nM	SANGER
IGROV-1	Growth Inhibition Assay				IC50=7.2 nM	SANGER
KYSE-270	Growth Inhibition Assay				IC50=7.21 nM	SANGER
TE-8	Growth Inhibition Assay				IC50=7.5 nM	SANGER
Daoy	Growth Inhibition Assay				IC50=8.07 nM	SANGER
REH	Growth Inhibition Assay				IC50=8.11 nM	SANGER
786-0	Growth Inhibition Assay				IC50=8.47 nM	SANGER
A375	Growth Inhibition Assay				IC50=9.17 nM	SANGER
EW-13	Growth Inhibition Assay				IC50=9.63 nM	SANGER
MKN1	Growth Inhibition Assay				IC50=9.86 nM	SANGER
MV-4-11	Growth Inhibition Assay				IC50=10.03 nM	SANGER
ESS-1	Growth Inhibition Assay				IC50=11.09 nM	SANGER
PSN1	Growth Inhibition Assay				IC50=11.22 nM	SANGER
MCF7	Growth Inhibition Assay				IC50=11.65 nM	SANGER
A427	Growth Inhibition Assay				IC50=11.81 nM	SANGER
NCI-H1651	Growth Inhibition Assay				IC50=11.83 nM	SANGER
LCLC-97TM1	Growth Inhibition Assay				IC50=11.83 nM	SANGER
DU-4475	Growth Inhibition Assay				IC50=12.1 nM	SANGER
SK-LU-1	Growth Inhibition Assay				IC50=12.54 nM	SANGER
NCI-H460	Growth Inhibition Assay				IC50=12.7 nM	SANGER
A204	Growth Inhibition Assay				IC50=12.88 nM	SANGER
AGS	Growth Inhibition Assay				IC50=13.27 nM	SANGER
NCI-H2228	Growth Inhibition Assay				IC50=13.48 nM	SANGER
HGC-27	Growth Inhibition Assay				IC50=13.56 nM	SANGER
RPMI-2650	Growth Inhibition Assay				IC50=13.69 nM	SANGER
ES1	Growth Inhibition Assay				IC50=13.72 nM	SANGER
MEL-JUSO	Growth Inhibition Assay				IC50=14.5 nM	SANGER
HT-1080	Growth Inhibition Assay				IC50=14.82 nM	SANGER
OCUB-M	Growth Inhibition Assay				IC50=15.15 nM	SANGER
23132-87	Growth Inhibition Assay				IC50=15.42 nM	SANGER
KYSE-510	Growth Inhibition Assay				IC50=15.48 nM	SANGER
ST486	Growth Inhibition Assay				IC50=15.53 nM	SANGER
639-V	Growth Inhibition Assay				IC50=15.91 nM	SANGER
COLO-679	Growth Inhibition Assay				IC50=15.97 nM	SANGER
CTV-1	Growth Inhibition Assay				IC50=16.07 nM	SANGER
BFTC-905	Growth Inhibition Assay				IC50=16.18 nM	SANGER
SW954	Growth Inhibition Assay				IC50=16.93 nM	SANGER
CAL-51	Growth Inhibition Assay				IC50=17.32 nM	SANGER
SW1710	Growth Inhibition Assay				IC50=17.4 nM	SANGER
HCC2998	Growth Inhibition Assay				IC50=17.44 nM	SANGER
A2058	Growth Inhibition Assay				IC50=17.65 nM	SANGER
HCE-4	Growth Inhibition Assay				IC50=18.07 nM	SANGER
MEL-HO	Growth Inhibition Assay				IC50=18.3 nM	SANGER
SNG-M	Growth Inhibition Assay				IC50=18.32 nM	SANGER
LS-123	Growth Inhibition Assay				IC50=18.46 nM	SANGER
NALM-6	Growth Inhibition Assay				IC50=18.63 nM	SANGER
NCI-H810	Growth Inhibition Assay				IC50=18.76 nM	SANGER
YKG-1	Growth Inhibition Assay				IC50=18.77 nM	SANGER
647-V	Growth Inhibition Assay				IC50=18.81 nM	SANGER
ONS-76	Growth Inhibition Assay				IC50=18.91 nM	SANGER
697	Growth Inhibition Assay				IC50=19.09 nM	SANGER
RS4-11	Growth Inhibition Assay				IC50=19.14 nM	SANGER
8505C	Growth Inhibition Assay				IC50=19.56 nM	SANGER
SK-UT-1	Growth Inhibition Assay				IC50=19.96 nM	SANGER
DOHH-2	Growth Inhibition Assay				IC50=19.99 nM	SANGER
NCCIT	Growth Inhibition Assay				IC50=20.13 nM	SANGER
LCLC-103H	Growth Inhibition Assay				IC50=20.54 nM	SANGER
CAL-12T	Growth Inhibition Assay				IC50=20.65 nM	SANGER
QIMR-WIL	Growth Inhibition Assay				IC50=20.72 nM	SANGER
SBC-5	Growth Inhibition Assay				IC50=20.74 nM	SANGER
769-P	Growth Inhibition Assay				IC50=20.76 nM	SANGER
A431	Growth Inhibition Assay				IC50=20.87 nM	SANGER
KE-37	Growth Inhibition Assay				IC50=21.05 nM	SANGER
ES8	Growth Inhibition Assay				IC50=21.07 nM	SANGER
22RV1	Growth Inhibition Assay				IC50=21.24 nM	SANGER
LOUCY	Growth Inhibition Assay				IC50=21.32 nM	SANGER
J82	Growth Inhibition Assay				IC50=21.34 nM	SANGER
MHH-ES-1	Growth Inhibition Assay				IC50=21.58 nM	SANGER
MG-63	Growth Inhibition Assay				IC50=22.08 nM	SANGER
8-MG-BA	Growth Inhibition Assay				IC50=22.12 nM	SANGER
BPH-1	Growth Inhibition Assay				IC50=22.12 nM	SANGER
RKO	Growth Inhibition Assay				IC50=22.28 nM	SANGER
HMV-II	Growth Inhibition Assay				IC50=22.6 nM	SANGER
NCI-H727	Growth Inhibition Assay				IC50=22.79 nM	SANGER
MOLT-4	Growth Inhibition Assay				IC50=22.86 nM	SANGER
NCI-H2342	Growth Inhibition Assay				IC50=22.91 nM	SANGER
MZ1-PC	Growth Inhibition Assay				IC50=23.81 nM	SANGER
TE-15	Growth Inhibition Assay				IC50=24.14 nM	SANGER
SR	Growth Inhibition Assay				IC50=24.41 nM	SANGER
CCRF-CEM	Growth Inhibition Assay				IC50=24.52 nM	SANGER
SIG-M5	Growth Inhibition Assay				IC50=25.69 nM	SANGER
SK-PN-DW	Growth Inhibition Assay				IC50=25.72 nM	SANGER
SK-HEP-1	Growth Inhibition Assay				IC50=25.86 nM	SANGER
SW620	Growth Inhibition Assay				IC50=26.63 nM	SANGER
HSC-2	Growth Inhibition Assay				IC50=26.78 nM	SANGER
CAL-148	Growth Inhibition Assay				IC50=26.9 nM	SANGER
COLO-205	Growth Inhibition Assay				IC50=26.99 nM	SANGER
EoL-1-cell	Growth Inhibition Assay				IC50=27.78 nM	SANGER
KYSE-150	Growth Inhibition Assay				IC50=27.78 nM	SANGER
G-361	Growth Inhibition Assay				IC50=28.88 nM	SANGER
PANC-03-27	Growth Inhibition Assay				IC50=28.16 nM	SANGER
NCI-H2030	Growth Inhibition Assay				IC50=28.26 nM	SANGER
NCI-H441	Growth Inhibition Assay				IC50=28.42 nM	SANGER
A388	Growth Inhibition Assay				IC50=28.46 nM	SANGER
BHY	Growth Inhibition Assay				IC50=28.88 nM	SANGER
KALS-1	Growth Inhibition Assay				IC50=29.33 nM	SANGER
NKM-1	Growth Inhibition Assay				IC50=29.47 nM	SANGER
BB30-HNC	Growth Inhibition Assay				IC50=29.85 nM	SANGER
G-401	Growth Inhibition Assay				IC50=30.2 nM	SANGER
EW-7	Growth Inhibition Assay				IC50=30.63 nM	SANGER
CAN	Growth Inhibition Assay				IC50=31.12 nM	SANGER
M059J	Growth Inhibition Assay				IC50=31.37 nM	SANGER
SK-MES-1	Growth Inhibition Assay				IC50=31.5 nM	SANGER
5637	Growth Inhibition Assay				IC50=31.87 nM	SANGER
MKN28	Growth Inhibition Assay				IC50=31.91 nM	SANGER
COR-L23	Growth Inhibition Assay				IC50=31.96 nM	SANGER
CAL-27	Growth Inhibition Assay				IC50=32.57 nM	SANGER
CAL-62	Growth Inhibition Assay				IC50=32.79 nM	SANGER
C-33-A	Growth Inhibition Assay				IC50=32.89 nM	SANGER
RT-112	Growth Inhibition Assay				IC50=32.92 nM	SANGER
KU812	Growth Inhibition Assay				IC50=33.24 nM	SANGER
C8166	Growth Inhibition Assay				IC50=33.31 nM	SANGER
VA-ES-BJ	Growth Inhibition Assay				IC50=33.36 nM	SANGER
NEC8	Growth Inhibition Assay				IC50=33.61 nM	SANGER
EB2	Growth Inhibition Assay				IC50=33.73 nM	SANGER
COLO-680N	Growth Inhibition Assay				IC50=33.87 nM	SANGER
IGR-1	Growth Inhibition Assay				IC50=33.83 nM	SANGER
MZ2-MEL	Growth Inhibition Assay				IC50=33.92 nM	SANGER
MIA-PaCa-2	Growth Inhibition Assay				IC50=34.24 nM	SANGER
IMR-5	Growth Inhibition Assay				IC50=34.68 nM	SANGER
CHP-212	Growth Inhibition Assay				IC50=34.96 nM	SANGER
CCF-STTG1	Growth Inhibition Assay				IC50=35.5 nM	SANGER
SK-MEL-30	Growth Inhibition Assay				IC50=35.61 nM	SANGER
HLE	Growth Inhibition Assay				IC50=35.8 nM	SANGER
HOP-62	Growth Inhibition Assay				IC50=35.88 nM	SANGER
LB1047-RCC	Growth Inhibition Assay				IC50=35.97 nM	SANGER
DoTc2-4510	Growth Inhibition Assay				IC50=36.25 nM	SANGER
HT-29	Growth Inhibition Assay				IC50=36.57 nM	SANGER
HC-1	Growth Inhibition Assay				IC50=37.32 nM	SANGER
TE-5	Growth Inhibition Assay				IC50=37.48 nM	SANGER
NCI-H1299	Growth Inhibition Assay				IC50=37.59 nM	SANGER
CHL-1	Growth Inhibition Assay				IC50=37.73 nM	SANGER
NCI-H2122	Growth Inhibition Assay				IC50=37.9 nM	SANGER
DEL	Growth Inhibition Assay				IC50=38.44 nM	SANGER
ES5	Growth Inhibition Assay				IC50=39.22 nM	SANGER
CESS	Growth Inhibition Assay				IC50=39.36 nM	SANGER
NCI-H719	Growth Inhibition Assay				IC50=39.65 nM	SANGER
A3-KAW	Growth Inhibition Assay				IC50=40.14 nM	SANGER
KP-4	Growth Inhibition Assay				IC50=40.77 nM	SANGER
NCI-H358	Growth Inhibition Assay				IC50=41.08 nM	SANGER
HCC38	Growth Inhibition Assay				IC50=42.5 nM	SANGER
MRK-nu-1	Growth Inhibition Assay				IC50=43.66 nM	SANGER
NCI-H292	Growth Inhibition Assay				IC50=43.76 nM	SANGER
PA-1	Growth Inhibition Assay				IC50=44.48 nM	SANGER
CAL-85-1	Growth Inhibition Assay				IC50=44.52 nM	SANGER
KYSE-450	Growth Inhibition Assay				IC50=44.71 nM	SANGER
IST-MEL1	Growth Inhibition Assay				IC50=44.84 nM	SANGER
Ca-Ski	Growth Inhibition Assay				IC50=44.84 nM	SANGER
OCI-AML2	Growth Inhibition Assay				IC50=45.05 nM	SANGER
NUGC-3	Growth Inhibition Assay				IC50=45.2 nM	SANGER
HOS	Growth Inhibition Assay				IC50=45.29 nM	SANGER
NB1	Growth Inhibition Assay				IC50=45.99 nM	SANGER
A253	Growth Inhibition Assay				IC50=46.12 nM	SANGER
NCI-H209	Growth Inhibition Assay				IC50=48.73 nM	SANGER
MC-CAR	Growth Inhibition Assay				IC50=48.74 nM	SANGER
NCI-H661	Growth Inhibition Assay				IC50=49.03 nM	SANGER
SF295	Growth Inhibition Assay				IC50=49.08 nM	SANGER
GP5d	Growth Inhibition Assay				IC50=49.12 nM	SANGER
P12-ICHIKAWA	Growth Inhibition Assay				IC50=49.27 nM	SANGER
ACHN	Growth Inhibition Assay				IC50=49.34 nM	SANGER
NCI-H2087	Growth Inhibition Assay				IC50=49.42 nM	SANGER
SW48	Growth Inhibition Assay				IC50=49.72 nM	SANGER
NCI-H526	Growth Inhibition Assay				IC50=49.96 nM	SANGER
Ca9-22	Growth Inhibition Assay				IC50=50.07 nM	SANGER
SK-OV-3	Growth Inhibition Assay				IC50=50.12 nM	SANGER
KYSE-140	Growth Inhibition Assay				IC50=50.42 nM	SANGER
BE-13	Growth Inhibition Assay				IC50=51.02 nM	SANGER
ES7	Growth Inhibition Assay				IC50=51.4 nM	SANGER
IM-9	Growth Inhibition Assay				IC50=51.43 nM	SANGER
A101D	Growth Inhibition Assay				IC50=51.48 nM	SANGER
CAPAN-1	Growth Inhibition Assay				IC50=51.76 nM	SANGER
NCI-H2009	Growth Inhibition Assay				IC50=51.76 nM	SANGER
NCI-H522	Growth Inhibition Assay				IC50=51.93 nM	SANGER
HuP-T4	Growth Inhibition Assay				IC50=52.22 nM	SANGER
BCPAP	Growth Inhibition Assay				IC50=52.5 nM	SANGER
NCI-H1734	Growth Inhibition Assay				IC50=53 nM	SANGER
SK-LMS-1	Growth Inhibition Assay				IC50=53.22 nM	SANGER
CGTH-W-1	Growth Inhibition Assay				IC50=53.46 nM	SANGER
RPMI-8402	Growth Inhibition Assay				IC50=53.86 nM	SANGER
Calu-3	Growth Inhibition Assay				IC50=55.98 nM	SANGER
COR-L279	Growth Inhibition Assay				IC50=56.95 nM	SANGER
T84	Growth Inhibition Assay				IC50=57.06 nM	SANGER
P30-OHK	Growth Inhibition Assay				IC50=57.57 nM	SANGER
CHP-126	Growth Inhibition Assay				IC50=59.24 nM	SANGER
KYSE-180	Growth Inhibition Assay				IC50=59.75 nM	SANGER
CAMG	Growth Inhibition Assay				IC50=59.96 nM	SANGER
EW-1	Growth Inhibition Assay				IC50=61.35 nM	SANGER
OVCAR-8	Growth Inhibition Assay				IC50=61.92 nM	SANGER
HL-60	Growth Inhibition Assay				IC50=62.56 nM	SANGER
8305C	Growth Inhibition Assay				IC50=63.12 nM	SANGER
IA-LM	Growth Inhibition Assay				IC50=63.54 nM	SANGER
HCC2157	Growth Inhibition Assay				IC50=63.95 nM	SANGER
CTB-1	Growth Inhibition Assay				IC50=64019 nM	SANGER
NCI-H1437	Growth Inhibition Assay				IC50=64.64 nM	SANGER
U251	Growth Inhibition Assay				IC50=64.89 nM	SANGER
ALL-PO	Growth Inhibition Assay				IC50=65.03 nM	SANGER
NCI-SNU-1	Growth Inhibition Assay				IC50=65.86 nM	SANGER
RPMI-6666	Growth Inhibition Assay				IC50=65.99 nM	SANGER
DBTRG-05MG	Growth Inhibition Assay				IC50=67.11 nM	SANGER
PF-382	Growth Inhibition Assay				IC50=67.17 nM	SANGER
GT3TKB	Growth Inhibition Assay				IC50=67.19 nM	SANGER
SCC-3	Growth Inhibition Assay				IC50=67.28 nM	SANGER
LU-134-A	Growth Inhibition Assay				IC50=67.68 nM	SANGER
CAL-33	Growth Inhibition Assay				IC50=67.87 nM	SANGER
MC-IXC	Growth Inhibition Assay				IC50=67.9 nM	SANGER
CPC-N	Growth Inhibition Assay				IC50=68.4 nM	SANGER
A2780	Growth Inhibition Assay				IC50=68.79 nM	SANGER
Daudi	Growth Inhibition Assay				IC50=68.96 nM	SANGER
GI-1	Growth Inhibition Assay				IC50=69.65 nM	SANGER
SW962	Growth Inhibition Assay				IC50=69.95 nM	SANGER
MFE-296	Growth Inhibition Assay				IC50=70.49 nM	SANGER
H4	Growth Inhibition Assay				IC50=71.56 nM	SANGER
AN3-CA	Growth Inhibition Assay				IC50=72.32 nM	SANGER
HCC1599	Growth Inhibition Assay				IC50=72.54 nM	SANGER
HuCCT1	Growth Inhibition Assay				IC50=72.93 nM	SANGER
Calu-1	Growth Inhibition Assay				IC50=73.6 nM	SANGER
DU-145	Growth Inhibition Assay				IC50=73.73 nM	SANGER
IST-SL2	Growth Inhibition Assay				IC50=73.81 nM	SANGER
HSC-3	Growth Inhibition Assay				IC50=74.53 nM	SANGER
HCC1806	Growth Inhibition Assay				IC50=74.96 nM	SANGER
VM-CUB-1	Growth Inhibition Assay				IC50=75.36 nM	SANGER
HSC-4	Growth Inhibition Assay				IC50=75.76 nM	SANGER
NH-12	Growth Inhibition Assay				IC50=76 nM	SANGER
MDA-MB-468	Growth Inhibition Assay				IC50=76.18 nM	SANGER
TUR	Growth Inhibition Assay				IC50=76.32 nM	SANGER
TE-1	Growth Inhibition Assay				IC50=76.35 nM	SANGER
CMK	Growth Inhibition Assay				IC50=77.42 nM	SANGER
LB996-RCC	Growth Inhibition Assay				IC50=78.45 nM	SANGER
NCI-H1650	Growth Inhibition Assay				IC50=78.62 nM	SANGER
DMS-273	Growth Inhibition Assay				IC50=78.9 nM	SANGER
NOS-1	Growth Inhibition Assay				IC50=79.14 nM	SANGER
NB69	Growth Inhibition Assay				IC50=79.2 nM	SANGER
SUP-T1	Growth Inhibition Assay				IC50=79.72 nM	SANGER
A172	Growth Inhibition Assay				IC50=80.55 nM	SANGER
TE-12	Growth Inhibition Assay				IC50=81.06 nM	SANGER
MKN45	Growth Inhibition Assay				IC50=81.51 nM	SANGER
LoVo	Growth Inhibition Assay				IC50=81.82 nM	SANGER
SW1573	Growth Inhibition Assay				IC50=82.81 nM	SANGER
ETK-1	Growth Inhibition Assay				IC50=83.01 nM	SANGER
ABC-1	Growth Inhibition Assay				IC50=83.45 nM	SANGER
COLO-668	Growth Inhibition Assay				IC50=84.47 nM	SANGER
UM-UC-3	Growth Inhibition Assay				IC50=86.02 nM	SANGER
BC-1	Growth Inhibition Assay				IC50=86.76 nM	SANGER
PANC-10-05	Growth Inhibition Assay				IC50=88.45 nM	SANGER
ES3	Growth Inhibition Assay				IC50=89.48 nM	SANGER
TE-441-T	Growth Inhibition Assay				IC50=89.82 nM	SANGER
KM-H2	Growth Inhibition Assay				IC50=90.68 nM	SANGER
SH-4	Growth Inhibition Assay				IC50=92.11 nM	SANGER
SF268	Growth Inhibition Assay				IC50=92.42 nM	SANGER
VMRC-RCZ	Growth Inhibition Assay				IC50=93.26 nM	SANGER
GI-ME-N	Growth Inhibition Assay				IC50=93.33 nM	SANGER
JEG-3	Growth Inhibition Assay				IC50=95.12 nM	SANGER
J-RT3-T3-5	Growth Inhibition Assay				IC50=96.31 nM	SANGER
K5	Growth Inhibition Assay				IC50=96.9 nM	SANGER
EFO-27	Growth Inhibition Assay				IC50=97.44 nM	SANGER
SW1783	Growth Inhibition Assay				IC50=97.99 nM	SANGER
CAL-39	Growth Inhibition Assay				IC50=98.24 nM	SANGER
LS-441N	Growth Inhibition Assay				IC50=99.06 nM	SANGER
G-402	Growth Inhibition Assay				IC50=99.09 nM	SANGER
MES-SA	Growth Inhibition Assay				IC50=99.53 nM	SANGER
FADU	Growth Inhibition Assay				IC50=102.18 nM	SANGER
DJM-1	Growth Inhibition Assay				IC50=102.25 nM	SANGER
SU-DHL-1	Growth Inhibition Assay				IC50=102.5 nM	SANGER
KYSE-410	Growth Inhibition Assay				IC50=103.46 nM	SANGER
NCI-H1618	Growth Inhibition Assay				IC50=104.35 nM	SANGER
AU565	Growth Inhibition Assay				IC50=104.51 nM	SANGER
SF126	Growth Inhibition Assay				IC50=104.58 nM	SANGER
MMAC-SF	Growth Inhibition Assay				IC50=106.48 nM	SANGER
ChaGo-K-1	Growth Inhibition Assay				IC50=107.95 nM	SANGER
NCI-H596	Growth Inhibition Assay				IC50=108.01 nM	SANGER
LXF-289	Growth Inhibition Assay				IC50=109.24 nM	SANGER
HCC1569	Growth Inhibition Assay				IC50=109.34 nM	SANGER
NCI-H2170	Growth Inhibition Assay				IC50=109.92 nM	SANGER
ARH-77	Growth Inhibition Assay				IC50=109.99 nM	SANGER
BOKU	Growth Inhibition Assay				IC50=110.87 nM	SANGER
SJSA-1	Growth Inhibition Assay				IC50=111.05 nM	SANGER
SW626	Growth Inhibition Assay				IC50=111.71 nM	SANGER
EHEB	Growth Inhibition Assay				IC50=112.59 nM	SANGER
TE-10	Growth Inhibition Assay				IC50=112.68 nM	SANGER
MZ7-mel	Growth Inhibition Assay				IC50=112.84 nM	SANGER
SAS	Growth Inhibition Assay				IC50=114.45 nM	SANGER
TK10	Growth Inhibition Assay				IC50=114.6 nM	SANGER
SW13	Growth Inhibition Assay				IC50=115.26 nM	SANGER
HCC1937	Growth Inhibition Assay				IC50=115.84 nM	SANGER
SK-NEP-1	Growth Inhibition Assay				IC50=116.26 nM	SANGER
OVCAR-5	Growth Inhibition Assay				IC50=116.81 nM	SANGER
SW837	Growth Inhibition Assay				IC50=117.95 nM	SANGER
BFTC-909	Growth Inhibition Assay				IC50=118.48 nM	SANGER
SW872	Growth Inhibition Assay				IC50=120.81 nM	SANGER
SF539	Growth Inhibition Assay				IC50=121.72 nM	SANGER
MLMA	Growth Inhibition Assay				IC50=122.08 nM	SANGER
BL-41	Growth Inhibition Assay				IC50=122.22 nM	SANGER
LB647-SCLC	Growth Inhibition Assay				IC50=123.35 nM	SANGER
NCI-H1703	Growth Inhibition Assay				IC50=126.42 nM	SANGER
KURAMOCHI	Growth Inhibition Assay				IC50=126.76 nM	SANGER
ATN-1	Growth Inhibition Assay				IC50=127.7 nM	SANGER
SK-CO-1	Growth Inhibition Assay				IC50=129.21 nM	SANGER
RPMI-7951	Growth Inhibition Assay				IC50=130.14 nM	SANGER
NCI-H1648	Growth Inhibition Assay				IC50=131.94 nM	SANGER
NCI-H64	Growth Inhibition Assay				IC50=132.65 nM	SANGER
HT-3	Growth Inhibition Assay				IC50=133.04 nM	SANGER
NCI-H1770	Growth Inhibition Assay				IC50=133.21 nM	SANGER
NB13	Growth Inhibition Assay				IC50=134.55 nM	SANGER
NCI-H747	Growth Inhibition Assay				IC50=135.02 nM	SANGER
ML-2	Growth Inhibition Assay				IC50=135.16 nM	SANGER
CA46	Growth Inhibition Assay				IC50=136.88 nM	SANGER
ES6	Growth Inhibition Assay				IC50=137.12 nM	SANGER
JiyoyeP-2003	Growth Inhibition Assay				IC50=137.8 nM	SANGER
NMC-G1	Growth Inhibition Assay				IC50=139.13 nM	SANGER
U-698-M	Growth Inhibition Assay				IC50=139.5 nM	SANGER
LU-99A	Growth Inhibition Assay				IC50=146.55 nM	SANGER
NCI-H748	Growth Inhibition Assay				IC50=147.31 nM	SANGER
SK-MEL-3	Growth Inhibition Assay				IC50=147.61 nM	SANGER
SNB75	Growth Inhibition Assay				IC50=148.65 nM	SANGER
BB65-RCC	Growth Inhibition Assay				IC50=149.31 nM	SANGER
NCI-H1355	Growth Inhibition Assay				IC50=152.24 nM	SANGER
SCC-25	Growth Inhibition Assay				IC50=153.73 nM	SANGER
OAW-42	Growth Inhibition Assay				IC50=153.84 nM	SANGER
MONO-MAC-6	Growth Inhibition Assay				IC50=154.36 nM	SANGER
WSU-NHL	Growth Inhibition Assay				IC50=154.58 nM	SANGER
SNU-423	Growth Inhibition Assay				IC50=155.34 nM	SANGER
SBC-1	Growth Inhibition Assay				IC50=156.4 nM	SANGER
OVCAR-4	Growth Inhibition Assay				IC50=156.78 nM	SANGER
NCI-H2347	Growth Inhibition Assay				IC50=158.34 nM	SANGER
CAMA-1	Growth Inhibition Assay				IC50=159.33 nM	SANGER
GDM-1	Growth Inhibition Assay				IC50=159.7 nM	SANGER
KNS-81-FD	Growth Inhibition Assay				IC50=160.32 nM	SANGER
no-10	Growth Inhibition Assay				IC50=160.38 nM	SANGER
NCI-H2052	Growth Inhibition Assay				IC50=160.47 nM	SANGER
DB	Growth Inhibition Assay				IC50=161.71 nM	SANGER
A4-Fuk	Growth Inhibition Assay				IC50=162.48 nM	SANGER
HCE-T	Growth Inhibition Assay				IC50=164.87 nM	SANGER
HuP-T3	Growth Inhibition Assay				IC50=165.55 nM	SANGER
BC-3	Growth Inhibition Assay				IC50=165.86 nM	SANGER
RVH-421	Growth Inhibition Assay				IC50=169.13 nM	SANGER
COLO-800	Growth Inhibition Assay				IC50=171.8 nM	SANGER
OMC-1	Growth Inhibition Assay				IC50=172.17 nM	SANGER
SJRH30	Growth Inhibition Assay				IC50=172.94 nM	SANGER
HEL	Growth Inhibition Assay				IC50=175.15 nM	SANGER
KLE	Growth Inhibition Assay				IC50=175.47 nM	SANGER
MEG-01	Growth Inhibition Assay				IC50=176.6 nM	SANGER
OAW-28	Growth Inhibition Assay				IC50=178.84 nM	SANGER
RD	Growth Inhibition Assay				IC50=180.1 nM	SANGER
HCC2218	Growth Inhibition Assay				IC50=181.66 nM	SANGER
OS-RC-2	Growth Inhibition Assay				IC50=182.49 nM	SANGER
HEC-1	Growth Inhibition Assay				IC50=182.83 nM	SANGER
SCC-4	Growth Inhibition Assay				IC50=183.71 nM	SANGER
ME-180	Growth Inhibition Assay				IC50=186.08 nM	SANGER
NB10	Growth Inhibition Assay				IC50=188.09 nM	SANGER
SCC-15	Growth Inhibition Assay				IC50=188.24 nM	SANGER
COR-L105	Growth Inhibition Assay				IC50=188.92 nM	SANGER
HT-1376	Growth Inhibition Assay				IC50=189.01 nM	SANGER
RPMI-8866	Growth Inhibition Assay				IC50=192.13 nM	SANGER
NCI-H2126	Growth Inhibition Assay				IC50=192.2 nM	SANGER
KARPAS-422	Growth Inhibition Assay				IC50=193.41 nM	SANGER
COLO-741	Growth Inhibition Assay				IC50=194.58 nM	SANGER
IPC-298	Growth Inhibition Assay				IC50=196.21 nM	SANGER
OE19	Growth Inhibition Assay				IC50=200.18 nM	SANGER
NCI-H1694	Growth Inhibition Assay				IC50=200.26 nM	SANGER
SW1990	Growth Inhibition Assay				IC50=201.9 nM	SANGER
LNCaP-Clone-FGC	Growth Inhibition Assay				IC50=204.47 nM	SANGER
LB831-BLC	Growth Inhibition Assay				IC50=206.25 nM	SANGER
HCT-116	Growth Inhibition Assay				IC50=207.39 nM	SANGER
BxPC-3	Growth Inhibition Assay				IC50=210.96 nM	SANGER
HT-1197	Growth Inhibition Assay				IC50=212.58 nM	SANGER
GB-1	Growth Inhibition Assay				IC50=212.73 nM	SANGER
RPMI-8226	Growth Inhibition Assay				IC50=213.37 nM	SANGER
L-540	Growth Inhibition Assay				IC50=213.49 nM	SANGER
NCI-H1882	Growth Inhibition Assay				IC50=215 nM	SANGER
A673	Growth Inhibition Assay				IC50=215.79 nM	SANGER
NCI-H650	Growth Inhibition Assay				IC50=217.61 nM	SANGER
KM12	Growth Inhibition Assay				IC50=218.15 nM	SANGER
KP-N-YS	Growth Inhibition Assay				IC50=218.4 nM	SANGER
COLO-684	Growth Inhibition Assay				IC50=219.79 nM	SANGER
MHH-NB-11	Growth Inhibition Assay				IC50=220.22 nM	SANGER
HO-1-N-1	Growth Inhibition Assay				IC50=222.83 nM	SANGER
NOMO-1	Growth Inhibition Assay				IC50=224.43 nM	SANGER
KYSE-70	Growth Inhibition Assay				IC50=224.66 nM	SANGER
NCI-H838	Growth Inhibition Assay				IC50=224.73 nM	SANGER
NY	Growth Inhibition Assay				IC50=228.49 nM	SANGER
NCI-H2196	Growth Inhibition Assay				IC50=229.15 nM	SANGER
FTC-133	Growth Inhibition Assay				IC50=229.37 nM	SANGER
MHH-PREB-1	Growth Inhibition Assay				IC50=229.82 nM	SANGER
SK-MEL-2	Growth Inhibition Assay				IC50=230.3 nM	SANGER
Detroit562	Growth Inhibition Assay				IC50=230.72 nM	SANGER
NCI-H2405	Growth Inhibition Assay				IC50=234.1 nM	SANGER
NCI-H2081	Growth Inhibition Assay				IC50=234.27 nM	SANGER
LB2241-RCC	Growth Inhibition Assay				IC50=235.11 nM	SANGER
SNU-387	Growth Inhibition Assay				IC50=236.08 nM	SANGER
PC-14	Growth Inhibition Assay				IC50=236.12 nM	SANGER
CAS-1	Growth Inhibition Assay				IC50=238.31 nM	SANGER
TE-11	Growth Inhibition Assay				IC50=240.88 nM	SANGER
LC4-1	Growth Inhibition Assay				IC50=240.89 nM	SANGER
HH	Growth Inhibition Assay				IC50=241.46 nM	SANGER
JVM-2	Growth Inhibition Assay				IC50=242.17 nM	SANGER
KNS-42	Growth Inhibition Assay				IC50=242.67 nM	SANGER
T-24	Growth Inhibition Assay				IC50=245.48 nM	SANGER
D-392MG	Growth Inhibition Assay				IC50=248.5 nM	SANGER
KOSC-2	Growth Inhibition Assay				IC50=248.71 nM	SANGER
D-283MED	Growth Inhibition Assay				IC50=250.13 nM	SANGER
BV-173	Growth Inhibition Assay				IC50=250.77 nM	SANGER
EW-18	Growth Inhibition Assay				IC50=250.9 nM	SANGER
MS-1	Growth Inhibition Assay				IC50=252.53 nM	SANGER
Calu-6	Growth Inhibition Assay				IC50=253.55 nM	SANGER
GR-ST	Growth Inhibition Assay				IC50=255.42 nM	SANGER
GAK	Growth Inhibition Assay				IC50=256.91 nM	SANGER
TE-9	Growth Inhibition Assay				IC50=257.47 nM	SANGER
HuH-7	Growth Inhibition Assay				IC50=258.44 nM	SANGER
KYSE-520	Growth Inhibition Assay				IC50=260.77 nM	SANGER
NCI-H520	Growth Inhibition Assay				IC50=266.61 nM	SANGER
HD-MY-Z	Growth Inhibition Assay				IC50=272.79 nM	SANGER
SW756	Growth Inhibition Assay				IC50=274.78 nM	SANGER
NB12	Growth Inhibition Assay				IC50=277.12 nM	SANGER
EW-3	Growth Inhibition Assay				IC50=279.89 nM	SANGER
AM-38	Growth Inhibition Assay				IC50=281.52 nM	SANGER
NCI-H23	Growth Inhibition Assay				IC50=283.92 nM	SANGER
GOTO	Growth Inhibition Assay				IC50=285.56 nM	SANGER
NCI-H1693	Growth Inhibition Assay				IC50=290.32 nM	SANGER
MOLT-16	Growth Inhibition Assay				IC50=290.5 nM	SANGER
HOP-92	Growth Inhibition Assay				IC50=290.7 nM	SANGER
MDA-MB-361	Growth Inhibition Assay				IC50=292.65 nM	SANGER
Ramos-2G6-4C10	Growth Inhibition Assay				IC50=294.55 nM	SANGER
CAL-54	Growth Inhibition Assay				IC50=300.73 nM	SANGER
D-263MG	Growth Inhibition Assay				IC50=305.52 nM	SANGER
SKG-IIIa	Growth Inhibition Assay				IC50=311.14 nM	SANGER
HAL-01	Growth Inhibition Assay				IC50=313.44 nM	SANGER
LAN-6	Growth Inhibition Assay				IC50=315.11 nM	SANGER
NCI-H1304	Growth Inhibition Assay				IC50=321.17 nM	SANGER
no-11	Growth Inhibition Assay				IC50=321.64 nM	SANGER
IST-MES1	Growth Inhibition Assay				IC50=323.35 nM	SANGER
L-363	Growth Inhibition Assay				IC50=324.43 nM	SANGER
EM-2	Growth Inhibition Assay				IC50=325.41 nM	SANGER
DV-90	Growth Inhibition Assay				IC50=329.45 nM	SANGER
Saos-2	Growth Inhibition Assay				IC50=329.94 nM	SANGER
UMC-11	Growth Inhibition Assay				IC50=331.28 nM	SANGER
Raji	Growth Inhibition Assay				IC50=334.64 nM	SANGER
RXF393	Growth Inhibition Assay				IC50=335.43 nM	SANGER
EW-11	Growth Inhibition Assay				IC50=341.56 nM	SANGER
BL-70	Growth Inhibition Assay				IC50=342.01 nM	SANGER
LB373-MEL-D	Growth Inhibition Assay				IC50=344.64 nM	SANGER
LU-139	Growth Inhibition Assay				IC50=344.92 nM	SANGER
U-87-MG	Growth Inhibition Assay				IC50=346.04 nM	SANGER
LU-65	Growth Inhibition Assay				IC50=346.4 nM	SANGER
U031	Growth Inhibition Assay				IC50=353.87 nM	SANGER
HN	Growth Inhibition Assay				IC50=354.27 nM	SANGER
MHH-CAL-2	Growth Inhibition Assay				IC50=359.21 nM	SANGER
NCI-H510A	Growth Inhibition Assay				IC50=359.59 nM	SANGER
NB5	Growth Inhibition Assay				IC50=359.81 nM	SANGER
KG-1	Growth Inhibition Assay				IC50=361.52 nM	SANGER
EFO-21	Growth Inhibition Assay				IC50=361.85 nM	SANGER
UACC-257	Growth Inhibition Assay				IC50=362.05 nM	SANGER
SW1417	Growth Inhibition Assay				IC50=363.23 nM	SANGER
NCI-H1417	Growth Inhibition Assay				IC50=370.04 nM	SANGER
DSH1	Growth Inhibition Assay				IC50=372.72 nM	SANGER
CAKI-1	Growth Inhibition Assay				IC50=375.48 nM	SANGER
SIMA	Growth Inhibition Assay				IC50=381.86 nM	SANGER
LS-1034	Growth Inhibition Assay				IC50=384.51 nM	SANGER
K-562	Growth Inhibition Assay				IC50=385.8 nM	SANGER
LOXIMVI	Growth Inhibition Assay				IC50=389.19 nM	SANGER
MC116	Growth Inhibition Assay				IC50=391.41 nM	SANGER
MPP-89	Growth Inhibition Assay				IC50=392.94 nM	SANGER
SN12C	Growth Inhibition Assay				IC50=394.87 nM	SANGER
BHT-101	Growth Inhibition Assay				IC50=395.92 nM	SANGER
DMS-114	Growth Inhibition Assay				IC50=396.31 nM	SANGER
NCI-H1838	Growth Inhibition Assay				IC50=405.05 nM	SANGER
HT	Growth Inhibition Assay				IC50=405.15 nM	SANGER
Caov-3	Growth Inhibition Assay				IC50=406.19 nM	SANGER
LB2518-MEL	Growth Inhibition Assay				IC50=413.1 nM	SANGER
C3A	Growth Inhibition Assay				IC50=415.79 nM	SANGER
NCI-H226	Growth Inhibition Assay				IC50=416.64 nM	SANGER
KMS-12-PE	Growth Inhibition Assay				IC50=418.06 nM	SANGER
RERF-LC-MS	Growth Inhibition Assay				IC50=425.05 nM	SANGER
OE33	Growth Inhibition Assay				IC50=426.02 nM	SANGER
COLO-678	Growth Inhibition Assay				IC50=427.18 nM	SANGER
NCI-H1963	Growth Inhibition Assay				IC50=430.11 nM	SANGER
LAMA-84	Growth Inhibition Assay				IC50=437.3 nM	SANGER
NCI-H2171	Growth Inhibition Assay				IC50=440.67 nM	SANGER
U-266	Growth Inhibition Assay				IC50=451.49 nM	SANGER
SNU-C2B	Growth Inhibition Assay				IC50=460.97 nM	SANGER
T47D	Growth Inhibition Assay				IC50=469.69 nM	SANGER
TE-6	Growth Inhibition Assay				IC50=469.81 nM	SANGER
OVCAR-3	Growth Inhibition Assay				IC50=470.41 nM	SANGER
C-4-II	Growth Inhibition Assay				IC50=472.56 nM	SANGER
EFM-19	Growth Inhibition Assay				IC50=478.27 nM	SANGER
NCI-N87	Growth Inhibition Assay				IC50=479.92 nM	SANGER
NCI-H187	Growth Inhibition Assay				IC50=493.21 nM	SANGER
MDA-MB-157	Growth Inhibition Assay				IC50=499.01 nM	SANGER
OPM-2	Growth Inhibition Assay				IC50=501.81 nM	SANGER
COLO-320-HSR	Growth Inhibition Assay				IC50=513.16 nM	SANGER
RMG-I	Growth Inhibition Assay				IC50=513.98 nM	SANGER
IST-SL1	Growth Inhibition Assay				IC50=517.42 nM	SANGER
UACC-893	Growth Inhibition Assay				IC50=521.51 nM	SANGER
Becker	Growth Inhibition Assay				IC50=521.83 nM	SANGER
HPAF-II	Growth Inhibition Assay				IC50=527.24 nM	SANGER
HCC1954	Growth Inhibition Assay				IC50=540.83 nM	SANGER
SNU-449	Growth Inhibition Assay				IC50=548.01 nM	SANGER
NCI-H1563	Growth Inhibition Assay				IC50=556.49 nM	SANGER
HDLM-2	Growth Inhibition Assay				IC50=568.59 nM	SANGER
NCI-H69	Growth Inhibition Assay				IC50=568.8 nM	SANGER
NCI-SNU-5	Growth Inhibition Assay				IC50=570.15 nM	SANGER
NCI-H1792	Growth Inhibition Assay				IC50=572.43 nM	SANGER
NCI-H2141	Growth Inhibition Assay				IC50=573.69 nM	SANGER
KMOE-2	Growth Inhibition Assay				IC50=576.32 nM	SANGER
NCI-H1155	Growth Inhibition Assay				IC50=579.87 nM	SANGER
LK-2	Growth Inhibition Assay				IC50=581.07 nM	SANGER
HCC1187	Growth Inhibition Assay				IC50=591.47 nM	SANGER
EGI-1	Growth Inhibition Assay				IC50=598.27 nM	SANGER
NCI-H1623	Growth Inhibition Assay				IC50=604.6 nM	SANGER
MN-60	Growth Inhibition Assay				IC50=605.37 nM	SANGER
JAR	Growth Inhibition Assay				IC50=625.95 nM	SANGER
HCC1419	Growth Inhibition Assay				IC50=631.74 nM	SANGER
LU-165	Growth Inhibition Assay				IC50=639.4 nM	SANGER
TYK-nu	Growth Inhibition Assay				IC50=662.55 nM	SANGER
DG-75	Growth Inhibition Assay				IC50=665.83 nM	SANGER
PFSK-1	Growth Inhibition Assay				IC50=678.08 nM	SANGER
MKN7	Growth Inhibition Assay				IC50=689.95 nM	SANGER
DMS-153	Growth Inhibition Assay				IC50=698.15 nM	SANGER
HTC-C3	Growth Inhibition Assay				IC50=703.94 nM	SANGER
GCIY	Growth Inhibition Assay				IC50=725.12 nM	SANGER
NCI-H1092	Growth Inhibition Assay				IC50=737.95 nM	SANGER
EB-3	Growth Inhibition Assay				IC50=771.02 nM	SANGER
CAL-120	Growth Inhibition Assay				IC50=777.52 nM	SANGER
BT-474	Growth Inhibition Assay				IC50=778.81 nM	SANGER
L-428	Growth Inhibition Assay				IC50=790.9 nM	SANGER
Mo-T	Growth Inhibition Assay				IC50=804.65 nM	SANGER
CAL-72	Growth Inhibition Assay				IC50=840.67 nM	SANGER
AsPC-1	Growth Inhibition Assay				IC50=855.26 nM	SANGER
U-118-MG	Growth Inhibition Assay				IC50=856.34 nM	SANGER
GCT	Growth Inhibition Assay				IC50=861.48 nM	SANGER
EKVX	Growth Inhibition Assay				IC50=871.87 nM	SANGER
NCI-H1755	Growth Inhibition Assay				IC50=873.52 nM	SANGER
Capan-2	Growth Inhibition Assay				IC50=898.11 nM	SANGER
NCI-H1793	Growth Inhibition Assay				IC50=900.87 nM	SANGER
PLC-PRF-5	Growth Inhibition Assay				IC50=901.35 nM	SANGER
M14	Growth Inhibition Assay				IC50=905.27 nM	SANGER
SK-MEL-24	Growth Inhibition Assay				IC50=914.35 nM	SANGER
SK-MM-2	Growth Inhibition Assay				IC50=942.3 nM	SANGER
PC-3	Growth Inhibition Assay				IC50=957.22 nM	SANGER
EW-24	Growth Inhibition Assay				IC50=970.4 nM	SANGER
MDA-MB-175-VII	Growth Inhibition Assay				IC50=971.53 nM	SANGER
NCI-H1581	Growth Inhibition Assay				IC50=981.31 nM	SANGER
THP-1	Growth Inhibition Assay				IC50=989.42 nM	SANGER
NCI-H1993	Growth Inhibition Assay				IC50=1.00076 μM	SANGER
DOK	Growth Inhibition Assay				IC50=1.01762 μM	SANGER
KINGS-1	Growth Inhibition Assay				IC50=1.019 μM	SANGER
YT	Growth Inhibition Assay				IC50=1.04568 μM	SANGER
TGBC1TKB	Growth Inhibition Assay				IC50=1.06036 μM	SANGER
TCCSUP	Growth Inhibition Assay				IC50=1.23083 μM	SANGER
BEN	Growth Inhibition Assay				IC50=1.23742 μM	SANGER
KARPAS-45	Growth Inhibition Assay				IC50=1.26763 μM	SANGER
COLO-829	Growth Inhibition Assay				IC50=1.2748 μM	SANGER
UACC-62	Growth Inhibition Assay				IC50=1.32145 μM	SANGER
NCI-H446	Growth Inhibition Assay				IC50=1.33771 μM	SANGER
RO82-W-1	Growth Inhibition Assay				IC50=1.34337 μM	SANGER
NCI-H2452	Growth Inhibition Assay				IC50=1.37129 μM	SANGER
RL	Growth Inhibition Assay				IC50=1.42066 μM	SANGER
NCI-H1522	Growth Inhibition Assay				IC50=1.43159 μM	SANGER
TGW	Growth Inhibition Assay				IC50=1.45942 μM	SANGER
HT55	Growth Inhibition Assay				IC50=1.47168 μM	SANGER
KARPAS-299	Growth Inhibition Assay				IC50=1.47841 μM	SANGER
KY821	Growth Inhibition Assay				IC50=1.49429 μM	SANGER
MDA-MB-231	Growth Inhibition Assay				IC50=1.53055 μM	SANGER
NCI-H82	Growth Inhibition Assay				IC50=1.57026 μM	SANGER
MDA-MB-134-VI	Growth Inhibition Assay				IC50=1.61711 μM	SANGER
PANC-08-13	Growth Inhibition Assay				IC50=1.63084 μM	SANGER
D-542MG	Growth Inhibition Assay				IC50=1.65305 μM	SANGER
LC-1F	Growth Inhibition Assay				IC50=1.66203 μM	SANGER
KP-N-YN	Growth Inhibition Assay				IC50=1.66343 μM	SANGER
MFE-280	Growth Inhibition Assay				IC50=1.66812 μM	SANGER
S-117	Growth Inhibition Assay				IC50=1.70504 μM	SANGER
NCI-H1573	Growth Inhibition Assay				IC50=1.71733 μM	SANGER
BT-20	Growth Inhibition Assay				IC50=1.74068 μM	SANGER
NCI-H28	Growth Inhibition Assay				IC50=1.74078 μM	SANGER
CaR-1	Growth Inhibition Assay				IC50=1.78324 μM	SANGER
LN-405	Growth Inhibition Assay				IC50=1.80143 μM	SANGER
RT4	Growth Inhibition Assay				IC50=1.84417 μM	SANGER
NCI-H889	Growth Inhibition Assay				IC50=1.93886 μM	SANGER
SW1463	Growth Inhibition Assay				IC50=1.95701 μM	SANGER
SCLC-21H	Growth Inhibition Assay				IC50=2.00624 μM	SANGER
CP66-MEL	Growth Inhibition Assay				IC50=2.01767 μM	SANGER
Hs-578-T	Growth Inhibition Assay				IC50=2.01782 μM	SANGER
HCC70	Growth Inhibition Assay				IC50=2.05463 μM	SANGER
SW1088	Growth Inhibition Assay				IC50=2.06931 μM	SANGER
NB6	Growth Inhibition Assay				IC50=2.185 μM	SANGER
KU-19-19	Growth Inhibition Assay				IC50=2.26917 μM	SANGER
A704	Growth Inhibition Assay				IC50=2.29135 μM	SANGER
EVSA-T	Growth Inhibition Assay				IC50=2.30089 μM	SANGER
HCT-15	Growth Inhibition Assay				IC50=2.35466 μM	SANGER
HuO-3N1	Growth Inhibition Assay				IC50=2.36583 μM	SANGER
GMS-10	Growth Inhibition Assay				IC50=2.39738 μM	SANGER
TGBC24TKB	Growth Inhibition Assay				IC50=2.41429 μM	SANGER
C2BBe1	Growth Inhibition Assay				IC50=2.51328 μM	SANGER
SK-N-DZ	Growth Inhibition Assay				IC50=2.56592 μM	SANGER
SK-N-AS	Growth Inhibition Assay				IC50=2.73015 μM	SANGER
T98G	Growth Inhibition Assay				IC50=2.80401 μM	SANGER
DMS-79	Growth Inhibition Assay				IC50=2.90988 μM	SANGER
SNU-475	Growth Inhibition Assay				IC50=2.92572 μM	SANGER
COLO-792	Growth Inhibition Assay				IC50=3.0287 μM	SANGER
YAPC	Growth Inhibition Assay				IC50=3.32832 μM	SANGER
UACC-812	Growth Inhibition Assay				IC50=3.33738 μM	SANGER
Mewo	Growth Inhibition Assay				IC50=3.46086 μM	SANGER
COLO-824	Growth Inhibition Assay				IC50=3.57378 μM	SANGER
SCC-9	Growth Inhibition Assay				IC50=3.91309 μM	SANGER
CP50-MEL-B	Growth Inhibition Assay				IC50=3.98703 μM	SANGER
NCI-H345	Growth Inhibition Assay				IC50=4.01174 μM	SANGER
COR-L88	Growth Inhibition Assay				IC50=4.3958 μM	SANGER
RCM-1	Growth Inhibition Assay				IC50=4.49272 μM	SANGER
JVM-3	Growth Inhibition Assay				IC50=4.60618 μM	SANGER
ECC4	Growth Inhibition Assay				IC50=4.76541 μM	SANGER
MDA-MB-453	Growth Inhibition Assay				IC50=4.85134 μM	SANGER
SK-N-FI	Growth Inhibition Assay				IC50=5.22877 μM	SANGER
D-502MG	Growth Inhibition Assay				IC50=5.24262 μM	SANGER
NCI-H2227	Growth Inhibition Assay				IC50=5.36231 μM	SANGER
DMS-53	Growth Inhibition Assay				IC50=6.17106 μM	SANGER
LB771-HNC	Growth Inhibition Assay				IC50=6.46806 μM	SANGER
BB49-HNC	Growth Inhibition Assay				IC50=7.41791 μM	SANGER
MDA-MB-415	Growth Inhibition Assay				IC50=7.57303 μM	SANGER
TALL-1	Growth Inhibition Assay				IC50=7.9688 μM	SANGER
HCC1395	Growth Inhibition Assay				IC50=8.15122 μM	SANGER
EFE-184	Growth Inhibition Assay				IC50=8.37216 μM	SANGER
EC-GI-10	Growth Inhibition Assay				IC50=8.68686 μM	SANGER
TT	Growth Inhibition Assay				IC50=9.06966 μM	SANGER
U-2-OS	Growth Inhibition Assay				IC50=9.81393 μM	SANGER
SHP-77	Growth Inhibition Assay				IC50=9.9029 μM	SANGER
HCC1143	Growth Inhibition Assay				IC50=10.4797 μM	SANGER
A498	Growth Inhibition Assay				IC50=18.2976 μM	SANGER
LS-513	Growth Inhibition Assay				IC50=18.6093 μM	SANGER
K052	Growth Inhibition Assay				IC50=21.4418 μM	SANGER
SiHa	Growth Inhibition Assay				IC50=23.3371 μM	SANGER
RCC10RGB	Growth Inhibition Assay				IC50=30.0611 μM	SANGER
NCI-SNU-16	Growth Inhibition Assay				IC50=31.1836 μM	SANGER
SNU-C1	Growth Inhibition Assay				IC50=32.4175 μM	SANGER
C32	Growth Inhibition Assay				IC50=32.872 μM	SANGER
SW684	Growth Inhibition Assay				IC50=34.1561 μM	SANGER
SK-MEL-28	Growth Inhibition Assay				IC50=35.5377 μM	SANGER
SK-MEL-1	Growth Inhibition Assay				IC50=36.7547 μM	SANGER
NCI-H720	Growth Inhibition Assay				IC50=41.9726 μM	SANGER
P31-FUJ	Growth Inhibition Assay				IC50=44.3284 μM	SANGER
NCI-H524	Growth Inhibition Assay				IC50=48.8248 μM	SANGER
Click to View More Cell Line Experimental Data

Mechanism of Action

Description

Targets

AMPK ^[6]

Topo II ^[1]
(Tumor cells)

In vitro
In vitro	Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. ^[2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. ^[3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. ^[5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. ^[6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor, indicating that Doxorubicin may also exert its effect by damaging proteins. ^[8]
Cell Research	Cell lines	ID8 cells
	Concentrations	5 uM
	Incubation Time	24 h
	Method	Parental ID8 or erastin-resistant ID8 cells were treated with different concentrations of doxorubicin for 24 hours.
Experimental Result Images	Methods	Biomarkers	Images	PMID
	Western blot	POU5F1 / SOX2 / NANOG ATG5 / ATG7 / SQSTM1 / LC3A-II / LC3B-II / p-mTOR P62 / LC3II p-AKT(T308) / p-Akt(S473) / Akt / p-PRAS40(T246) / PRAS40 / p-H2A.X(S139)		27929731
	Growth inhibition assay	Cell viability		25871400
	Immunofluorescence	LC3		24970805
	ELISA	OPN		24963635

In Vivo
In vivo	In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. ^[1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. ^[2]
Animal Research	Animal Models	Female athymic nude mice injected s.c. with MB231 cells
	Dosages	3 mg/kg/day
	Administration	Delivered intratumorly

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-05-22)
NCT06186700	Active not recruiting	Breast Cancer Female	Mansoura University	December 25 2023	Phase 2
NCT05567601	Not yet recruiting	Ovarian Cancer\|AIDS-related Kaposi Sarcoma\|Multiple Myeloma\|Metastatic Breast Cancer	Baxter Healthcare Corporation	November 30 2023	Phase 1
NCT06088290	Recruiting	Leiomyosarcoma	PharmaMar	September 21 2023	Phase 2\|Phase 3
NCT05748834	Recruiting	Breast Cancer	SCRI Development Innovations LLC\|Seagen Inc.	July 24 2023	Phase 2
NCT05853120	Recruiting	Sexually Transmitted Diseases	Emory University\|Centers for Disease Control and Prevention	May 31 2023	Phase 4

References
https://pubmed.ncbi.nlm.nih.gov/19401447/ https://pubmed.ncbi.nlm.nih.gov/20385199/ https://pubmed.ncbi.nlm.nih.gov/21834791/ https://pubmed.ncbi.nlm.nih.gov/21364585/ https://pubmed.ncbi.nlm.nih.gov/22589537/ https://pubmed.ncbi.nlm.nih.gov/22267730/ https://pubmed.ncbi.nlm.nih.gov/22374332/ https://pubmed.ncbi.nlm.nih.gov/22536838/ https://pubmed.ncbi.nlm.nih.gov/19377506/ https://pubmed.ncbi.nlm.nih.gov/17194596/ https://pubmed.ncbi.nlm.nih.gov/17194596/ + Expand

References

https://pubmed.ncbi.nlm.nih.gov/19401447/
https://pubmed.ncbi.nlm.nih.gov/20385199/
https://pubmed.ncbi.nlm.nih.gov/21834791/

https://pubmed.ncbi.nlm.nih.gov/21364585/
https://pubmed.ncbi.nlm.nih.gov/22589537/
https://pubmed.ncbi.nlm.nih.gov/22267730/
https://pubmed.ncbi.nlm.nih.gov/22374332/
https://pubmed.ncbi.nlm.nih.gov/22536838/
https://pubmed.ncbi.nlm.nih.gov/19377506/
https://pubmed.ncbi.nlm.nih.gov/17194596/
https://pubmed.ncbi.nlm.nih.gov/17194596/

+ Expand

Chemical Information

Molecular Weight	579.98	Formula	C₂₇H₂₉NO₁₁.HCl
CAS No.	25316-40-9	SDF	Download Doxorubicin (DOX) HCl SDF
Synonyms	Adriamycin, NSC 123127, Hydroxydaunorubicin HCl
Smiles	CC1C(C(CC(O1)OC2CC(CC3=C2C(=C4C(=C3O)C(=O)C5=C(C4=O)C(=CC=C5)OC)O)(C(=O)CO)O)N)O.Cl

Storage and Stability

Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
			1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (172.41 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 100 mg/mL

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (8.62mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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If you have any other enquiries, please leave a message.

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Frequently Asked Questions

Question 1:
Why the IC50 of the compound varied over time in our measurement?

Answer:
Sometimes the IC50 value might vary between different batch of compounds due to the slight differences in purity. If different IC50 was seen in the same batch of compound, the problem may lies in the process of dissolvement. Make sure that you see no particles and the solution is clear with your stock solution.

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