Doxorubicin (Adriamycin) HCl

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127

Doxorubicin (Adriamycin) HCl Chemical Structure

Molecular Weight(MW): 579.98

Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.

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Cited by 33 Publications

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  • Enhancement of apoptotic response by addition of targeted agents lapatinib and sunitinib to doxorubicin in the same device reservoir at 24 hours. P values were determined using Student’s t test for all graphs.

    Sci Transl Med, 2015, 7(284): 284ra57 . Doxorubicin (Adriamycin) HCl purchased from Selleck.

    (F) Kaplan-Meier curves are shown for all groups described: no hydrogel, a hydrogel loaded with paclitaxel, or a hydrogel loaded with doxorubicin. The number of mice per group (n) and median survival (ms) are listed. The experiment was performed at least three times.

    Science, 2018, 10(433), doi: 10.1126/scitranslmed.aar1916. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • Cell viabilities with increasing concentrations of cisplatin (CP) and doxorubicin (DOXO) under normoxic and hypoxic condition for 48 hours were determined by MTT assay. IC50 values are presented as the means ?SDs (n=4) and * denotes p<0.05.

    Cancer Res 2014 74, 298-308. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Cleavage of caspase-3, -9 and the expression of PARP were measured by western blot using b-actin as a loading control.

    Cell Cycle, 2017, 16(5):468-476. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • (C) & (D) Treatments with doxorubicin, cisplatin or AZD6244 further decreased the cell viability in RH30 cloned cells in which STAT3 or GP130 was knockdown by shRNA. (*P<.05, ** P <.01, *** P <.001).

    Curr Cancer Drug Targets, 2016, 16(7):631-8. Doxorubicin (Adriamycin) HCl purchased from Selleck.

     

    Doxorubicin release of P(MAA-g-EG) and P(MAA-g-EG) containing nanoparticles in two-step pH conditions. Doxorubicinloaded P(MAA-g-EG) ,P(MAA-g-EG)-1.0NP ,P(MAA-g-EG)-2.5NP, and P(MAA-g-EG)-5.0NP crushed particles (75–150 um) were released in 1 PBS (pH 2.0) for 90 min. Then the pH was increased to 7.0 by adding 5N NaOH and release continued for 6 h.

    J Biomed Mater Res Part A 2012 10.1002/jbm.a.34064. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • Temporal uptake of free DOX or DOX-PCL63-b-PNVP90 micelles into K-562 (I) and DL (J) cells were studied by incubating in 24-well plates at a concentration of 50,000 cells per well. Representative images shown were obtained using a fluorescence microscope Eclipse 80i (Nikon, Japan) (Plan Fluor, 40X, NA 0.75 objective) equipped with blue and red filters for, Hoechst and Doxorubicin respectively (n = 3).

    PLoS One 2014 9, e94309. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    PLoS One 2013 8(1), e54595. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • CON+ -positive control (with FBS as chemoattractant); CON- - negative control (without chemoattractant); DOX -doxorubicin; WZ -WZ811; PF- PF-573228; * -significant difference compared to positive control: p<0.05; ** -significant difference compared to positive control: p<0.01; # -significant difference between combination and inhibitor only: p<0.05. Cancer cell invasion was analyzed using Transwell membranes assembled in 24-well plates and covered with a layer of Matrigel. COR-L23 cells were treated for 24 h with 50 nM DOX, 10 uM WZ811, 5 uM PF-573228 and combinations of inhibitors with 50 nM DOX. Similarly, RH460 cells were treated for 24 h with 1 uM DOX, 10 uM WZ811, 2.5 uM PF-573228 and combinations of inhibitors with 1 uM DOX. Applied doses were the ones that exerted the most pronounced synergistic effect on cytotoxicity level. After the incubation period, cells that invaded through matrigel and membranes were fixed in 4% paraformaldehyde-PBS and stained with Hoechst 33342 nuclear stain. Cells were visualized and counted under a fluorescent microscope at 10x magnification.

    2014 Dr Milica Pesic from Institute for Biological Research. Doxorubicin (Adriamycin) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.
Targets
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
L1210 NFnRSIFHfW6ldHnvckBCe3OjeR?= NETJ[YlKdmirYnn0bY9vKG:oIGLORUB{gW62aHXzbZMhf2m2aDDFSFUxKG:oIECuOVgh|ryP MUi0PVA2OzZ?
Yoshida sarcoma NHTMeXVHfW6ldHnvckBCe3OjeR?= NF[0fZhKdmirYnn0bY9vKG:oIGuzTH1V\FJiaX7jc5Jxd3KjdHnvckB4cXSqIFnDOVAhd2ZiMD6wNkDPxE1? NGTHTnEyOzJ{OUi2
CHO MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPWUGxZUUN3ME2wMlA3KM7:TR?= NH\PWGYyOzV2N{Ww
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8226/ADR M37XdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoD3TWM2OD1yLkW2JO69VQ>? M{HHdlE1PDd5M{C=
MCF7/ADR M4e0WGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFnR[mhKSzVyPUOuNUDPxE1? M1S4TVE1PDd5M{C=
H69 NYDXe49MT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX3nN4ZrUUN3ME2wMlg4KM7:TR?= NF[wUGsyPDd7NUi1
N417 NXX1U3pRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLJTWM2OD1yLkKzJO69VQ>? NVH4[I9iOTR5OUW4OS=>
H9 MUXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\KTY5pcWKrdHnvckBw\iCKOTDj[YxteyClaILvcolk[WyueTDpcoZm[3SnZDD3bZRpKHSqZTDITXYuOSBqSEmvTWlKSilid3n0bEBKTDVyIH;mJFAvODFizszN NEnoNW8yPTR6Nkix
T-C8166 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXeydnZWUUR3ME2wMlAyKM7:TR?= MX[xOVQ5Pjhz
T-H9 NWjUXHFvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWnJSFUxRTBwMEGg{txO NVjEb|NIOTV2OE[4NS=>
T-MT4 MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JSFUxRTBwMEGg{txO MkXpNVU1QDZ6MR?=
SKMEL-5 M2DFTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fsdmVFPTB;Mz6yJO69VQ>? M3TZdlE3OTN5NUO=
MLM NGPaWlFEgXSxdH;4bYMhSXO|YYm= NVTDW4p5TUR3ME2wMlE{KM7:TR?= M{H0e|E5PzV|NUC=
KBM-3 MoHIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX[1ZpdZUUN3ME2wMlAzPiEQvF2= NY\TcW9oOTl7NUi3Oy=>
HT-29 M4fMSmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHfZWVIxNjBzIN88[y=> NIfk[lQ1QCCq MXfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eR?= NVjyb3R6Ojd5OES0PS=>
EMT6 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGTiVmExNjVizszN NVW3VZJMOSCq M37nOWlvcGmkaYTpc44hd2ZicILvcIln\XKjdHnvckBjgSCvZXHzeZJqdmdiZn;yJJN2en[rdnnu[{BnemGldHnvcpMhf2m2aDDFSFUxKG:oIECuNFAxOSEQvF2= M{C0eFc1PTJ4N{S=
H2981 NX7tTJFvS3m2b4TvfIlkKEG|c3H5 NVLoWpljUUN3ME2wMlUh|ryP MVq3O|MyODJ|
LOX MnzkR5l1d3SxeHnjJGF{e2G7 NIjuc5pKSzVyPUCuNFQzKM7:TR?= M1L0WFc5PTN|M{G=
C38 MXzDfZRwfG:6aXOgRZN{[Xl? MUjJR|UxRTBwMEGg{txO MXu4NFU4Ojlz
EAC NYLMe4l6S3m2b4TvfIlkKEG|c3H5 NI\qNG04OiCq NGftdYhKSzVyPUCuN|Uh|ryP NXXTTFdKQDN3MEC4Oy=>
SKVLB M2HJWGN6fG:2b4jpZ{BCe3OjeR?= M{S5PWlEPTB;NT62JO69VQ>? NYG3Ump{QDV5NkmxOC=>
AUC375 MlrrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoT6TWM2OD1yLkGxNkDPxE1? NXfINFVqQDZ2OE[wNC=>
A2780-C25 NIezfG9EgXSxdH;4bYMhSXO|YYm= NXXlPXc5S3m2b4TvfIlkcXS7IHHnZYlve3Rib4jhcIlxdGG2aX6tdoV{cXO2YX70JGEzPzhyLVOyOUB4cXSqIFnDOVAhd2ZiMD6wOlMh|ryP Ml64PFg{OTd3NR?=
A2780-CP3 MUDDfZRwfG:6aXOgRZN{[Xl? M{[3bGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGOrc4DsZZRqdi2{ZYPpd5RidnRiQUK3PFAuS1B|IIfpeIghUUN3MDDv[kAxNjB3NjFOwG0> NH3wTFE5QDNzN{W1
A2780-DX5 M1PzZWN6fG:2b4jpZ{BCe3OjeR?= NGjyUFJEgXSxdH;4bYNqfHliYXfhbY5{fCCmb4jvdpVjcWOrbj3y[ZNqe3SjboSgRVI4QDBvRGi1JJdqfGhiSVO1NEBw\iByLkO1O{DPxE1? NWPCNZhsQDh|MUe1OS=>
MXF7 M2\YN2N6fG:2b4jpZ{BCe3OjeR?= MkLIRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4Sgd4Vve2m2aY\lJG1ZTjdid3n0bEBKSzVyIH;mJFAvODJ6IN88US=> NVXneHRxQDl4MEW1PC=>
MXF7 MlTXR5l1d3SxeHnjJGF{e2G7 M1u2VWFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JI1qfG:6YX70do9v\S2{ZYPpd5RidnRiTWjGO{B4cXSqIFnDOVAhd2ZiMD6wNlgh|ryP MUS4PVYxPTV6
MXF7 M2nDZ2N6fG:2b4jpZ{BCe3OjeR?= M3;2OGFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIRwgG:{dXLpZ4lvNXKnc3nzeIFvfCCPWF[3JJdqfGhiSVO1NEBw\iBzLkG3NkDPxE1? MW[4PVYxPTV6
WiDr M4GzdmN6fG:2b4jpZ{BCe3OjeR?= MXTBZ5Rqfmm2eTDh[4FqdnO2IIPlcpNqfGm4ZTDXbWRzKGOnbHyge4l1cCCLQ{WwJI9nKDBwMEWzJO69VQ>? NFXtdow5QTZyNUW4
MCF NXrwb451TnWwY4Tpc44hSXO|YYm= Mk\XVoV3\XK|YXygc4YhWC2pcD3t[YRq[XSnZDDteYx1cWS{dXegdoV{cXO2YX7j[UBie3Onc4Pl[EBieyCnbnjhcoNmdWWwdDDpckBkgXSxdH;4bYNqfHlib3[gZYRzcWGveXPpci=> Mn\yPFk5PDF3MR?=
C6 NGDjdnJEgXSxdH;4bYMhSXO|YYm= NEPYXmczNjVibXevcWw> NVznS4lbPzJiaB?= MX\EUXNQ MlLCR5l1d3SxeHnjbZR6KGGpYXnud5QhemG2IFO2JINmdGy|IHH0JFUxKHWpL33MJJRwKDJwNTDt[{9uVA>? M2PDTVg6QDh4MES=
C6 NV\1NnZNTnWwY4Tpc44hSXO|YYm= NHj2e|gzNjVibXevcWw> NYq4OJljPCCq M1zWbWROW09? NFryWWZKdmirYnn0bY9vKG:oIIT1ZpVtcW5icH;sfY1memm8YYTpc44> M33aNlg6QDh4MES=
C6 M2f0dGN6fG:2b4jpZ{BCe3OjeR?= NUTvUVRxOi53IH3nM41N MW[1JIg> NIOyZZdFVVOR M4W2RmN6fG:2b4jpZ4l1gSCjc4Pld5Nm\CCjczDj[YxtKHKnbHXhd4U> M1\lXlg6QDh4MES=
SKVLB NXy0eWJpS3m2b4TvfIlkKEG|c3H5 M17tbVczKGh? NIG5ZpJKSzVyPU[uOVQh|ryP MWe5NFA{PTJy
MCF7/VP16 MkC1R5l1d3SxeHnjJGF{e2G7 NILqNYo2OCEQvHevcWw> NUXwfo5DPzJiaB?= MV;JR|UxRTF2LkKg{txO NHPlRXc6ODJ{N{my
CA755 M4DCdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmK1NVAh|ryP M4i1VVYh\A>? M17PdGlEPTB;MD6wNVQh|ryP NIjnW4g6PTJ4NUew
CHO NWnIVlFyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkfxNVAh|ryP MWm2JIQ> MUnJR|UxRTBwME[g{txO NGHpVoU6PTJ4NUew
Panc02 NYDsWoF3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLnOVUyOCEQvF2= MYW2JIQ> MXrJR|UxRTBwME[1JO69VQ>? NEnoVWc6PTJ4NUew
PC14 M4e3OGN6fG:2b4jpZ{BCe3OjeR?= NWXwRVNWPCCm M1K0XGROW09? MnLTTWM2OD1yLkK5JO69VQ>? M3zvOVk5OjJ3NEK=
HOP62 MYDDfZRwfG:6aXOgRZN{[Xl? M3z6PFczKGh? M33CR2lEPTB;MD6wNFQh|ryP NFHnUlA6QDd|Nk[x
SF-295 MUXDfZRwfG:6aXOgRZN{[Xl? MXG3NkBp NWTyeIFZUUN3ME2wMlA{PSEQvF2= NH;qdJY6QDd|Nk[x
MDA-MB-361 NHXkPXFEgXSxdH;4bYMhSXO|YYm= NWfRcZhMOSCq Ml\CSG1UVw>? NYDGUWRpS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTBMW1DNTN4MTDlfJBz\XO|aX7nJGxi[1pid3n0bEBKSzVyIH;mJFAvODF{IN88US=> M1L0OFExOzl3NEmw
MDA-MB-361 MnSzR5l1d3SxeHnjJGF{e2G7 MX6xJIg> MknZSG1UVw>? NV\xWXlIS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTBJG1DKDN4MTDlfJBz\XO|aX7nJINiemKxeInw[ZB1cWSjc3Wgd5REWEd{IIfpeIghUUN3MDDv[kAxNjBzODFOwG0> NYexOJREOTB|OUW0PVA>
CCRF-SB M2\aTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2rYdlQh\A>? MYTEUXNQ Mn60TWM2OD1yLkCxJO69VQ>? MkDqNVA1OTF2N{[=
CHO-K1 MmLNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4HldFQh\A>? NVzicpM5TE2VTx?= MX\JR|UxRTBwNDFOwG0> MnPrNVA1OTF2N{[=
KB M4\3[2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\iUFQh\A>? M4H3ZWROW09? Mne2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDkdpVoNXKnc3nzeIFvfCCNQkfEJJdqfGhiSVO1NEBw\iByLk[1JO69VQ>? NVf4bGx2OTB2MUG0O|Y>
KB NF7nTHlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYThOnBWPCCm MV7EUXNQ NV7N[ZZTSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRmNidXC5aYToJGlEPTBib3[gNE4xPiEQvF2= NFPoTosyODRzMUS3Oi=>
KB NF;JcFBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYnXUY5CPCCm NILhSJFFVVOR NVHhXYpMSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRm1FWiC5aYToJGlEPTBib3[gNE42KM7:TR?= MX6xNFQyOTR5Nh?=
KB MnPmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlXMOEBl M{LlWmROW09? NF\ybFFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIRzfWdvcnXzbZN1[W62IFvCWlIxSyC5aYToJGlEPTBib3[gNE41KM7:TR?= NXnjXWxDOTB2MUG0O|Y>
HTB-54 Mom3R5l1d3SxeHnjJGF{e2G7 NYDsNJh3OTByIN88US=> NYW4eWt3UUN3ME2xMlEh|ryP NV[wSIl{OTB2N{[4OlE>
MEL-AC MV7DfZRwfG:6aXOgRZN{[Xl? M4T1dlExOCEQvF2= MX\JR|UxRTFwMjFOwG0> NUn0O2ZoOTB2N{[4OlE>
DU145 MX3DfZRwfG:6aXOgRZN{[Xl? NFzKRYw4OiCq NIHMUGNKSzVyPUCuNFUzQCEQvF2= NYfjWoF[OTB2N{myPFI>
K562R MXzDfZRwfG:6aXOgRZN{[Xl? M17zO|czKGh? NGTmT4dKSzVyPUCuNFE2PiEQvF2= M4HFOlExPDd7Mkiy
PACA-2 MmDvR5l1d3SxeHnjJGF{e2G7 NInBXXA3KGR? M4i0PWVFPTB;MD6wNFY{KM7:TR?= NYPVS4xIOTB2OUiyNVQ>
3Y1 MmHMR5l1d3SxeHnjJGF{e2G7 MV23NkBp M{i4OGdKPTB;MD6wNFM5KM7:TR?= NVzaclBDOTB4OUG3NVA>
P388 NHztPWhEgXSxdH;4bYMhSXO|YYm= M{HtO|czKGh? M{DBcGdKPTB;MD6wNVIh|ryP NFHGRmMyODZ7MUexNC=>
HMV-1 NHnhRZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlfLTWM2OD1yLkK4JO69VQ>? MknoNVA3QTh2M{[=
MKN-1 Mkm1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mk\uTWM2OD1yLk[0JO69VQ>? NGTtZYEyODZ7OESzOi=>
PC14 NXPPe3QxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2fNNmlEPTB;MT65JO69VQ>? M1n5WFExPjl6NEO2
T24 MlTMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUH5SWY4UUN3ME2wMlY1KM7:TR?= NEG2bpYyODZ7OESzOi=>
U937 NUPISJA{TnWwY4Tpc44hSXO|YYm= Mm\VNlQhcA>? MlXRSG1UVw>? MX7Jcohq[mm2czDpcoNwenCxcnH0bY9vKG:oIGuzTH11cHmvaXTpcoUhf2m2aDDJR|UxKG:oIECuNUDPxE1? MlXpNVA4QDB7MUG=
H460pv8/eto NEnxWmhEgXSxdH;4bYMhSXO|YYm= NWHpOXF3OTByIN88US=> MUO0PEBp M2q4SXNidGmwZR?= NIPPOFFKSzVyPUCuNFIh|ryP MYOxNFc5ODlzMx?=
H460pv8 NVnvXZpKS3m2b4TvfIlkKEG|c3H5 MV2xNFAh|ryP NX3XTIVSPDhiaB?= NF3kb4NU[WyrbnW= NXnpdnZVUUN3ME2wMlAxPCEQvF2= M4HEb|ExPzhyOUGz
MDA-231 NHPqdoREgXSxdH;4bYMhSXO|YYm= NEfXOpIyODBizszN Mm\0OFghcA>? MXfTZYxqdmV? M2P2PWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQT2yN|Eh[2WubDCoSXIuNEWJRmKrMI12fGGwdDDwOVMqKHerdHigTWM2OCCxZjCwMlAxPiEQvF2= MYCxNFc5ODlzMx?=
MDA-468 M1z3VGN6fG:2b4jpZ{BCe3OjeR?= MWexNFAh|ryP NVm2dJN2PDhiaB?= MonOV4FtcW6n MYLDfZRwfG:6aXPpeJkh[WejaX7zeEBOTEFvNE[4JINmdGxiKFXSMUwh[W2ybHnmbYVlKEWJRmKsJI12fGGwdDDwOVMqKHerdHigTWM2OCCxZjCwMlAxPSEQvF2= NH;nWXcyODd6MEmxNy=>
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NCI-H322 M37uPWN6fG:2b4jpZ{BCe3OjeR?= MXqxNFAh|ryP MkK4OFghcA>? M2nCSXNidGmwZR?= MoDWR5l1d3SxeHnjbZR6KGGpYXnud5QhVkOLLVizNlIh[2WubDCocZV1[W62IIC1N{khf2m2aDDJR|UxKG:oIECuNFMh|ryP MWWxNFc5ODlzMx?=
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CP66-MEL NFLBOmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTJwMEG3Olch|ryP MY\TRW5ITVJ?
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HCC70 NGW4Uo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NITZNZJKSzVyPUKuNFU1PjNizszN NIjIUY1USU6JRWK=
SW1088 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NELHe5NKSzVyPUKuNFY6OzFizszN MlvTV2FPT0WU
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KU-19-19 M{fwOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HqNGlEPTB;Mj6yOlkyPyEQvF2= NXHTVFJzW0GQR1XS
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C2BBe1 MnzrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rCTGlEPTB;Mj61NVMzQCEQvF2= NFiyVpZUSU6JRWK=
SK-N-DZ M2O4WWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWfjcJJtUUN3ME2yMlU3PTl{IN88US=> M3fPW3NCVkeHUh?=
SK-N-AS NIS0fpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTJwN{OwNVUh|ryP MWTTRW5ITVJ?
T98G NVL6SFlXT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3zVRWlEPTB;Mj64NFQxOSEQvF2= M1TyfXNCVkeHUh?=
DMS-79 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mnr0TWM2OD1{LkmwPVg5KM7:TR?= M{n3dnNCVkeHUh?=
SNU-475 NX7RW2trT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1\SXGlEPTB;Mj65NlU4OiEQvF2= Mkf2V2FPT0WU
COLO-792 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2jiTWlEPTB;Mz6wNlg4KM7:TR?= MXzTRW5ITVJ?
YAPC M3fDUGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHq1e|VKSzVyPUOuN|I5OzJizszN NIO1[YhUSU6JRWK=
UACC-812 M4P2c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXzwRm5YUUN3ME2zMlM{PzN6IN88US=> MVXTRW5ITVJ?
Mewo MYLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NITTRlBKSzVyPUOuOFYxQDZizszN NXPobll7W0GQR1XS
COLO-824 MnLkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXfJR|UxRTNwNUezO|gh|ryP NXrpbHJSW0GQR1XS
SCC-9 NITrfmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUDFOYtnUUN3ME2zMlkyOzB7IN88US=> M1zCR3NCVkeHUh?=
CP50-MEL-B NUjjd3kzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFjEeJRKSzVyPUOuPVg4ODNizszN MWnTRW5ITVJ?
NCI-H345 MknWS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1XkNGlEPTB;ND6wNVE4PCEQvF2= M1vrd3NCVkeHUh?=
COR-L88 NYPEOGFOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1rtdWlEPTB;ND6zPVU5KM7:TR?= M1\QR3NCVkeHUh?=
RCM-1 MnjtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4PadWlEPTB;ND60PVI4OiEQvF2= M1HzUnNCVkeHUh?=
JVM-3 NHzLXWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGCyd49KSzVyPUSuOlA3OThizszN NHjMR3lUSU6JRWK=
ECC4 M2LmW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYnLfpA{UUN3ME20Mlc3PTRzIN88US=> M2TneXNCVkeHUh?=
MDA-MB-453 NWDVdI5LT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXXBclhiUUN3ME20Mlg2OTN2IN88US=> NXHHUJRXW0GQR1XS
SK-N-FI MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlrUTWM2OD13LkKyPFc4KM7:TR?= NUP0c2xRW0GQR1XS
D-502MG M1jxZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTVwMkSyOlIh|ryP MV3TRW5ITVJ?
NCI-H2227 MkjZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIfzdHVKSzVyPUWuN|YzOzFizszN MWrTRW5ITVJ?
DMS-53 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M36yWmlEPTB;Nj6xO|ExPiEQvF2= MoS0V2FPT0WU
LB771-HNC NW\mOod6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX3JR|UxRTZwNE[4NFYh|ryP MlG1V2FPT0WU
BB49-HNC NI\hV3JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHq3dlNKSzVyPUeuOFE4QTFizszN NHyxW2xUSU6JRWK=
MDA-MB-415 NXXhe5hNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M333cGlEPTB;Nz61O|MxOyEQvF2= NX;BVYZVW0GQR1XS
TALL-1 M1nUT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NInLXlFKSzVyPUeuPVY5QCEQvF2= M3LiU3NCVkeHUh?=
HCC1395 MnXqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{DsbWlEPTB;OD6xOVEzOiEQvF2= M1nVfXNCVkeHUh?=
EFE-184 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkSzTWM2OD16LkO3NlE3KM7:TR?= MmHsV2FPT0WU
EC-GI-10 NX\KOZlFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXITWM2OD16Lk[4Olg3KM7:TR?= MXfTRW5ITVJ?
TT MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2r0c2lEPTB;OT6wOlk3PiEQvF2= M1G1bHNCVkeHUh?=
U-2-OS NFj1fW9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnfFTWM2OD17LkixN|k{KM7:TR?= M173NXNCVkeHUh?=
SHP-77 MmT6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnjnTWM2OD17LkmwNlkh|ryP M2PyW3NCVkeHUh?=
HCC1143 MoPOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{fB[mlEPTB;MUCuOFc6PyEQvF2= NFvxXmJUSU6JRWK=
A498 MojLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{jwWWlEPTB;MUiuNlk4PiEQvF2= MYLTRW5ITVJ?
LS-513 MkPoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIL0bYpKSzVyPUG4MlYxQTNizszN M2Lhd3NCVkeHUh?=
K052 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDmV|RTUUN3ME2yNU41PDF6IN88US=> NVPhemlTW0GQR1XS
SiHa MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MV7JR|UxRTJ|LkOzO|Eh|ryP MYDTRW5ITVJ?
RCC10RGB MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoDjTWM2OD1|MD6wOlEyKM7:TR?= MoLnV2FPT0WU
NCI-SNU-16 MmPIS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn7mTWM2OD1|MT6xPFM3KM7:TR?= MVrTRW5ITVJ?
SNU-C1 NIK5bIlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGfrbHBKSzVyPUOyMlQyPzVizszN MVvTRW5ITVJ?
C32 M{XPTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTN{Lki3NkDPxE1? MlOxV2FPT0WU
SW684 MX\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWO3NVl1UUN3ME2zOE4yPTZzIN88US=> M2\yO3NCVkeHUh?=
SK-MEL-28 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFzrTIdKSzVyPUO1MlU{PzdizszN M2PoWnNCVkeHUh?=
SK-MEL-1 MYDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXnJR|UxRTN4Lke1OFch|ryP M1HsUXNCVkeHUh?=
NCI-H720 NX63VXN[T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmLrTWM2OD12MT65O|I3KM7:TR?= MYDTRW5ITVJ?
P31-FUJ MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLKcoFKSzVyPUS0MlMzQDRizszN NV7ye|czW0GQR1XS
NCI-H524 Mo[3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTR6LkiyOFgh|ryP Ml\HV2FPT0WU

... Click to View More Cell Line Experimental Data

In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (172.41 mM)
Water 20 mg/mL (34.48 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl

CAS No. 25316-40-9
Storage powder
in solvent
Synonyms NSC 123127

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT01489371 Active not recruiting Ovarian Clear Cell Cystadenocarcinoma|Ovarian Endometrioid Adenocarcinoma|Ovarian Seromucinous Carcinoma|Ovarian Serous Cystadenocarcinoma|Recurrent Fallopian Tube Carcinoma|Recurrent Ovarian Carcinoma|Recurrent Primary Peritoneal Carcinoma|Undifferentiated Ovarian Carcinoma Gynecologic Oncology Group|National Cancer Institute (NCI) July 9 2012 Phase 1
NCT02751918 Recruiting Ovarian Neoplasms Bayer June 8 2016 Phase 1
NCT00722293 Completed Neoplasms Breast GlaxoSmithKline July 8 2008 Phase 1
NCT03301350 Suspended Breast Cancer|Triple Negative Breast Cancer University of Wisconsin Madison|National Cancer Institute (NCI) November 7 2017 Phase 2
NCT02979522 Recruiting Hodgkin Disease Millennium Pharmaceuticals Inc.|Takeda September 7 2017 Phase 1|Phase 2
NCT03399747 Enrolling by invitation Diffuse Large B-cell Lymphoma Asan Medical Center December 7 2016 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Why the IC50 of the compound varied over time in our measurement?

  • Answer:

    Sometimes the IC50 value might vary between different batch of compounds due to the slight differences in purity. If different IC50 was seen in the same batch of compound, the problem may lies in the process of dissolvement. Make sure that you see no particles and the solution is clear with your stock solution.

Topoisomerase Signaling Pathway Map

Related Topoisomerase Products

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID