Doxorubicin (Adriamycin) HCl

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127

Doxorubicin (Adriamycin) HCl Chemical Structure

Molecular Weight(MW): 579.98

Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.

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Cited by 602 Publications

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Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.
Targets
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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MLM MUXDfZRwfG:6aXOgRZN{[Xl? NWHNZ3VMTUR3ME2wMlE{KM7:TR?= M{X5XVE5PzV|NUC=
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SKVLB M3LqOGN6fG:2b4jpZ{BCe3OjeR?= M2j4V|czKGh? NUXY[HpnUUN3ME22MlU1KM7:TR?= NVPjZpJpQTByM{WyNC=>
MCF7/VP16 MoftR5l1d3SxeHnjJGF{e2G7 MlnvOVAh|rypL33M NF6wbIk4OiCq NIjweYdKSzVyPUG0MlIh|ryP NGHIPHQ6ODJ{N{my
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CHO MlSxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MnLzNVAh|ryP NHXVUYE3KGR? M4\pdWlEPTB;MD6wOkDPxE1? MX[5OVI3PTdy
Panc02 NFfKWHFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHXOPWQyOCEQvF2= M132VlYh\A>? MYLJR|UxRTBwME[1JO69VQ>? MY[5OVI3PTdy
PC14 MV3DfZRwfG:6aXOgRZN{[Xl? Mnu1OEBl M{fzWWROW09? MXvJR|UxRTBwMkmg{txO MVG5PFIzPTR{
HOP62 M4jBXmN6fG:2b4jpZ{BCe3OjeR?= M{WzNFczKGh? Mm\YTWM2OD1yLkCwOEDPxE1? NWDCcodGQTh5M{[2NS=>
SF-295 Mk\XR5l1d3SxeHnjJGF{e2G7 MkW4O|IhcA>? Mn3yTWM2OD1yLkCzOUDPxE1? Ml7TPVg4OzZ4MR?=
MDA-MB-361 M4HVd2N6fG:2b4jpZ{BCe3OjeR?= NX3SV4xQOSCq MV3EUXNQ NVPtS3ExS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTBMW1DNTN4MTDlfJBz\XO|aX7nJGxi[1pid3n0bEBKSzVyIH;mJFAvODF{IN88US=> Mn36NVA{QTV2OUC=
MDA-MB-361 NFm4bYNEgXSxdH;4bYMhSXO|YYm= NWjDVIVuOSCq MULEUXNQ MXzDfZRwfG:6aXPpeJkh[WejaX7zeEBOTEFiTVKgN|YyKGW6cILld5NqdmdiY3HyZo95gXCncITp[IF{\SC|dFPQS|Ihf2m2aDDJR|UxKG:oIECuNFE5KM7:TR?= MY[xNFM6PTR7MB?=
CCRF-SB NFnFNWNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVvYOWpUPCCm MUXEUXNQ NV22WItwUUN3ME2wMlAyKM7:TR?= MY[xNFQyOTR5Nh?=
CHO-K1 NX;lZoczT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH7RUo01KGR? M1m3cmROW09? MXjJR|UxRTBwNDFOwG0> NIjreGYyODRzMUS3Oi=>
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KB NV;Fc3FFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUPDW5A4PCCm NGflfG5FVVOR NGHPcJpCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fUBi\2GrboP0JIRzfWdvcnXzbZN1[W62IFvCR4FueHerdHigTWM2OCCxZjCwMlA3KM7:TR?= MnzaNVA1OTF2N{[=
KB M1fwOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVG0JIQ> MX\EUXNQ MonPRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDkdpVoNXKnc3nzeIFvfCCNQl3EVkB4cXSqIFnDOVAhd2ZiMD61JO69VQ>? M1TxOVExPDFzNEe2
KB M2jnS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmfXOEBl NHy5ZpFFVVOR MYXBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuEVkKwR{B4cXSqIFnDOVAhd2ZiMD60JO69VQ>? MkXHNVA1OTF2N{[=
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MEL-AC NH:xbJlEgXSxdH;4bYMhSXO|YYm= NECw[FMyODBizszN NXvTfWw2UUN3ME2xMlIh|ryP MYSxNFQ4Pjh4MR?=
DU145 M3rRSmN6fG:2b4jpZ{BCe3OjeR?= M{S1[|czKGh? NW\VVFRRUUN3ME2wMlA2OjhizszN M1;DR|ExPDd7Mkiy
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MCF7 NUG2WVJ1TnWwY4Tpc44hSXO|YYm= Mm\oOUDPxE1? NVrjcpZ6OjRiaB?= NWTDT3Q3S2WubIXsZZIhfXC2YXvl NEGwRW4zOzl5M{KxNy=>
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MDA-MB-231 M3TjRmZ2dmO2aX;uJGF{e2G7 MojNOUDPxE1? M2nXc|I1KGh? MXLD[YxtfWyjcjD1dJRic2V? NXq4WnhbOjN7N{OyNVM>
MDA-MB-231 Mn\VSpVv[3Srb36gRZN{[Xl? MlnxOUDPxE1? M1\PU|I1KGh? Mm\yRY51cW:6aXThcpQh[WO2aY\peJkh[XO|ZYPz[YQh[XNiaX70doFk\WyudXzhdkBu[Wyxbons[IlidGSnaInk[UBt\X[nbB?= NIDXW4QzOzl5M{KxNy=>
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HUVEC NHLTbm9EgXSxdH;4bYMhSXO|YYm= MVK0PEBp MkC2TWM2OD1yLkC4NFch|ryP MUCyOFAyOjN5OB?=
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MDA-MB-435 M4fOb2N6fG:2b4jpZ{BCe3OjeR?= MW[xNFAh|ryP NWO3UVJNPDhiaB?= MkfiTWM2OD1yLkG1JO69VQ>? M2SyTlI1ODJ6NES2
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Caov3 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mof4O|IhcA>? M1PK[WlEPTB;MD6xJO69VQ>? MUiyOFA6PTB6OR?=
LNcaP MUPGeY5kfGmxbjDBd5NigQ>? MXyzJO69VQ>? MkC4NlQhcA>? NH74S|RKdmO{ZXHz[UBw\iClbHXheoVlKGOjc4Dhd4UuQSCuZY\lcC=> NGOyTWczPDB7NUC4PS=>
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Caco2 MnzjR5l1d3SxeHnjJGF{e2G7 NW\WOYdjPDBizszN MVmyOEBp NFLyN4FEgXSxdH;4bYNqfHl? NVLQcGhXOjRyOUm5PVQ>
MDA-MB-468 NE\RbYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVuxNEDPxE1? MW[3NkBp NX:yO3pOT0l3ME2wMlA5PSEQvF2= M1TYfVI1OTZ|N{K5
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MKN28 NFLUS5pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYXlfo9PPDhiaB?= M{e0RWdKPTB;MD6yOEDPxE1? M16yWlI1OTh2MEe2
SGC7901 Ml\jR5l1d3SxeHnjJGF{e2G7 NVLuNXk6UUN3ME2wMlAyOSEQvF2= MVyyOFE6PTR4Nh?=
Bel7402 NVjJNW1CT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFe1epU6PiCq M{fYcGROW09? M{e5N2lEPTB;MD61O|gzKM7:TR?= NH3EdYkzPDJzMU[zPS=>
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MGC803 MkHIR5l1d3SxeHnjJGF{e2G7 NGW5clI4OiCq NEjTbHNKSzVyPUCuNVch|ryP MkDsNlQ{PzBzMUS=
ME180 M{[xfWN6fG:2b4jpZ{BCe3OjeR?= MWC0PEBp NEnZOmZKSzVyPUCuN|kh|ryP NX\LdI5tOjR2OEexPFg>
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GMS-10 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1fpWGlEPTB;Mj6zPVc{QCEQvF2= NUf1[|NSW0GQR1XS
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DMS-79 NVTVUlhFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVvJR|UxRTJwOUC5PFgh|ryP MlXMV2FPT0WU
SNU-475 NEDTbXJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NVix[WpHUUN3ME2yMlkzPTd{IN88US=> MVHTRW5ITVJ?
COLO-792 NHTXbYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4n3OWlEPTB;Mz6wNlg4KM7:TR?= NV\yPVFrW0GQR1XS
YAPC MlzOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mli0TWM2OD1|LkOyPFMzKM7:TR?= NV;tWVB6W0GQR1XS
UACC-812 MkS5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlnSTWM2OD1|LkOzO|M5KM7:TR?= NWDOWGlbW0GQR1XS
Mewo NH3rPGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXEZ2d6UUN3ME2zMlQ3ODh4IN88US=> NGfrelRUSU6JRWK=
COLO-824 NYjQ[5RxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVnqPGhPUUN3ME2zMlU4Ozd6IN88US=> NHuzVlBUSU6JRWK=
SCC-9 M1rhT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M17qc2lEPTB;Mz65NVMxQSEQvF2= NVXPUWZUW0GQR1XS
CP50-MEL-B NWr5blVFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGj5RXZKSzVyPUOuPVg4ODNizszN MYjTRW5ITVJ?
NCI-H345 NVXGR5FmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTRwMEGxO|Qh|ryP NUHTblFnW0GQR1XS
COR-L88 NFqzXI9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPZTWM2OD12LkO5OVgh|ryP NYXMTG5{W0GQR1XS
RCM-1 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3u5WGlEPTB;ND60PVI4OiEQvF2= Mm\IV2FPT0WU
JVM-3 M1fQSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXfJR|UxRTRwNkC2NVgh|ryP M2rjPXNCVkeHUh?=
ECC4 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2joTGlEPTB;ND63OlU1OSEQvF2= NYPTbY5KW0GQR1XS
MDA-MB-453 M1j2PWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV36d|h[UUN3ME20Mlg2OTN2IN88US=> NEftc3JUSU6JRWK=
SK-N-FI NW\YdXpbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkfpTWM2OD13LkKyPFc4KM7:TR?= NEjsRYtUSU6JRWK=
D-502MG MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NG[ySGlKSzVyPUWuNlQzPjJizszN Mk\iV2FPT0WU
NCI-H2227 MljLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHvBRVRKSzVyPUWuN|YzOzFizszN NGjacVlUSU6JRWK=
DMS-53 NXq4U|VyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnK4TWM2OD14LkG3NVA3KM7:TR?= NVG2ZlNmW0GQR1XS
LB771-HNC M3\5UWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkD6TWM2OD14LkS2PFA3KM7:TR?= NUXuWFF{W0GQR1XS
BB49-HNC MojkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDWb29KSzVyPUeuOFE4QTFizszN MUnTRW5ITVJ?
MDA-MB-415 NHH0UmJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NU[wcZhsUUN3ME23MlU4OzB|IN88US=> MlPWV2FPT0WU
TALL-1 MlziS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX7JR|UxRTdwOU[4PEDPxE1? MofiV2FPT0WU
HCC1395 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRThwMUWxNlIh|ryP NIPmV4FUSU6JRWK=
EFE-184 MX3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3qxd2lEPTB;OD6zO|IyPiEQvF2= MU\TRW5ITVJ?
EC-GI-10 M3TqT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVzJR|UxRThwNki2PFYh|ryP MoLDV2FPT0WU
TT MoPaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYC0[lQ5UUN3ME25MlA3QTZ4IN88US=> NH6zbJpUSU6JRWK=
U-2-OS NIXpNoJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW\5fnZ6UUN3ME25MlgyOzl|IN88US=> MWXTRW5ITVJ?
SHP-77 MmCwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTlwOUCyPUDPxE1? M1zQSnNCVkeHUh?=
HCC1143 NYnGe2x{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfvSoxYUUN3ME2xNE41Pzl5IN88US=> M1PqS3NCVkeHUh?=
A498 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF[zd4NKSzVyPUG4MlI6PzZizszN NF2xW|ZUSU6JRWK=
LS-513 NUDuflNYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NULMcWRrUUN3ME2xPE43ODl|IN88US=> MXTTRW5ITVJ?
K052 NGewb3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{LzTGlEPTB;MkGuOFQyQCEQvF2= NYLTU3o5W0GQR1XS
SiHa Mke2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHnTWM2OD1{Mz6zN|cyKM7:TR?= MUXTRW5ITVJ?
RCC10RGB NHXIeFVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXzJR|UxRTNyLkC2NVEh|ryP NGL0PWFUSU6JRWK=
NCI-SNU-16 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{\YfmlEPTB;M{GuNVg{PiEQvF2= M4\lNnNCVkeHUh?=
SNU-C1 MmG4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVPJR|UxRTN{LkSxO|Uh|ryP M2T4bHNCVkeHUh?=
C32 MnjXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3XaXGlEPTB;M{KuPFczKM7:TR?= NInpTGdUSU6JRWK=
SW684 MVfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XySGlEPTB;M{SuNVU3OSEQvF2= NVPkeIRYW0GQR1XS
SK-MEL-28 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlHmTWM2OD1|NT61N|c4KM7:TR?= NGjCWphUSU6JRWK=
SK-MEL-1 M1P6cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1vJS2lEPTB;M{[uO|U1PyEQvF2= MmDRV2FPT0WU
NCI-H720 M13wVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV7JR|UxRTRzLkm3NlYh|ryP MYLTRW5ITVJ?
P31-FUJ NXXCe4lMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGLQXItKSzVyPUS0MlMzQDRizszN Mn;VV2FPT0WU
NCI-H524 NXS2dG12T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXnJR|UxRTR6LkiyOFgh|ryP NXnkbolXW0GQR1XS

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
POU5F1 / SOX2 / NANOG; 

PubMed: 27929731     


NT2/D1 cells treated with 10 nM of doxorubicin were subjected to WB analysis for pluripotency factors (POU5F1, NANOG, SOX2) and differentiation markers (TUBB3).

ATG5 / ATG7 / SQSTM1 / LC3A-II / LC3B-II / p-mTOR; 

PubMed: 27929731     


NT2/D1 cells treated with 10 nM of doxorubicin were subjected to WB analysis for autophagy markers (ATG5, ATG7, SQSTM1, LC3A-II, LC3B-II and p-MTOR). 

P62 / LC3II; 

PubMed: 28208617     


(A,B) Doxorubicin induces autophagy in pancreatic cancer cells. Western blot analysis of LC3-II and p62 protein levels in Mia PaCa-2 and Panc-1 cell lines treated with indicated concentrations of doxorubicin for 24 h, or treated with 2.5 μM doxorubicin for indicated times.

p-AKT(T308) / p-Akt(S473) / Akt / p-PRAS40(T246) / PRAS40 / p-H2A.X(S139); 

PubMed: 26027929     


MCF10A cells were serum-starved and treated with (A) 2 μM doxorubicin over a 10 hour time course.

27929731 28208617 26027929
Growth inhibition assay
Cell viability; 

PubMed: 25871400     


Salinomycin enhances the cytotoxicity of doxorubicin in HCC cellsRelative cell viability (mean ± SD) for salinomycin (black), doxorubicin (blue) and doxorubicin plus salinomycin (red) in HuH-7, HepG2, SNU-449 and SNU-387 cells.

25871400
Immunofluorescence
LC3; 

PubMed: 24970805     


Doxorubicin induces GFP-LC3 puncta formation in hepatocellular carcinoma cells. SK-Hep1 cells were treated with doxorubicin (2.5 μM) or CQ (10 μM) plus doxorubicin for 7 h and cells were examined with a confocal microscope for GFP-LC3 punctuation/aggregation.

24970805
ELISA
OPN; 

PubMed: 24963635     


A549 were exposed to 50 nM doxorubicin or PBS for 48 h. OPN concentrations in cell culture media were determined by ELISA.

24963635
In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
- Collapse
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (172.41 mM)
Water 20 mg/mL (34.48 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl

CAS No. 25316-40-9
Storage powder
in solvent
Synonyms NSC 123127

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04032964 Not yet recruiting Drug: L19TNF|Drug: DOXORUBICIN Soft Tissue Sarcoma Philogen S.p.A. September 2019 Phase 1
NCT03975205 Not yet recruiting Drug: Doxorubicin Lymphoma Leukemia Sutphin Drugs|Bharath Charitable Cancer Hospital and Institute July 1 2019 Early Phase 1
NCT03591276 Recruiting Drug: Chemotherapy Drugs Cancer Metastatic Breast Cancer Shaare Zedek Medical Center April 18 2019 Phase 1|Phase 2
NCT03719430 Recruiting Drug: Doxorubicin|Drug: APX005M Soft Tissue Sarcoma Columbia University|Apexigen Inc. March 20 2019 Phase 2
NCT03059615 Recruiting Drug: Nerofe|Drug: Doxorubicin Acute Myelogenous Leukemia|Myelodysplastic Syndromes Immune System Key Ltd October 25 2018 Phase 2

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Why the IC50 of the compound varied over time in our measurement?

  • Answer:

    Sometimes the IC50 value might vary between different batch of compounds due to the slight differences in purity. If different IC50 was seen in the same batch of compound, the problem may lies in the process of dissolvement. Make sure that you see no particles and the solution is clear with your stock solution.

Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID