Doxorubicin (Adriamycin) HCl

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127

Doxorubicin (Adriamycin) HCl Chemical Structure

Molecular Weight(MW): 579.98

Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.

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Cited by 88 Publications

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Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.
Targets
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
L1210 MYHGeY5kfGmxbjDBd5NigQ>? M1;vOGlvcGmkaYTpc44hd2ZiUl7BJJN6dnSqZYPpd{B4cXSqIFXEOVAhd2ZiMD61PEDPxE1? NXHqVHJFPDlyNUO2
Yoshida sarcoma NIfzO5ZHfW6ldHnvckBCe3OjeR?= M3yyWmlvcGmkaYTpc44hd2ZiW{PIYXRlWiCrbnPvdpBwemG2aX;uJJdqfGhiSVO1NEBw\iByLkCyJO69VQ>? MVGxN|IzQTh4
CHO MlzjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIjyepNKSzVyPUCuNFYh|ryP NEXKUokyOzV2N{Ww
8226 M3SwSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjpV4Y4UUN3ME2wMlA{OyEQvF2= M4HnblE1PDd5M{C=
8226/ADR MkPXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2XpO2lEPTB;MD61OkDPxE1? M1fCTVE1PDd5M{C=
MCF7/ADR MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF23OI5KSzVyPUOuNUDPxE1? MXexOFQ4PzNy
H69 NHniPHhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2XtbmlEPTB;MD64O{DPxE1? MmnWNVQ4QTV6NR?=
N417 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTBwMkOg{txO MWKxOFc6PTh3
H9 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIX4bnJKdmirYnn0bY9vKG:oIFi5JINmdGy|IHPodo9vcWOjbHz5JIlv\mWldHXkJJdqfGhidHjlJGhKXi1zIDjIPU9KUUmEKTD3bZRpKEmGNUCgc4YhOC5yMTFOwG0> M37U[FE2PDh4OEG=
T-C8166 MmXoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWrFPJFDUUR3ME2wMlAyKM7:TR?= MWixOVQ5Pjhz
T-H9 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmD0TWQ2OD1yLkCxJO69VQ>? M16zc|E2PDh4OEG=
T-MT4 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmHKTWQ2OD1yLkCxJO69VQ>? NES2bXQyPTR6Nkix
SKMEL-5 MonCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmLlSWQ2OD1|LkKg{txO NI\FW4cyPjF|N{Wz
MLM NGrLWplEgXSxdH;4bYMhSXO|YYm= M2nyTWVFPTB;MD6xN{DPxE1? M{K3Z|E5PzV|NUC=
KBM-3 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVnlOmk1UUN3ME2wMlAzPiEQvF2= NUfpWGZtOTl7NUi3Oy=>
HT-29 M{LpcWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXfZcIdZOC5yMTFOwIc> NUDNUYNZPDhiaB?= M4q4[GFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5 M2\jbFI4Pzh2NEm=
EMT6 NH6wOolIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\6Sog{OC53IN88US=> NFHLNlEyKGh? NXHFWHFuUW6qaXLpeIlwdiCxZjDwdo9tcW[ncnH0bY9vKGK7IH3lZZN2emmwZzDmc5Ihe3W{dnn2bY5oKG[{YXP0bY9veyC5aYToJGVFPTBib3[gNE4xODBzIN88US=> M4W4[lc1PTJ4N{S=
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A2780-DX5 M4P6[WN6fG:2b4jpZ{BCe3OjeR?= M363TWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGSxeH;yeYJq[2mwLYLld4l{fGGwdDDBNlc5OC2GWEWge4l1cCCLQ{WwJI9nKDBwM{W3JO69VQ>? MVO4PFMyPzV3
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MXF7 M{jDSWN6fG:2b4jpZ{BCe3OjeR?= M3PCe2FvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIRwgG:{dXLpZ4lvNXKnc3nzeIFvfCCPWF[3JJdqfGhiSVO1NEBw\iBzLkG3NkDPxE1? MWe4PVYxPTV6
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MCF M33LPGZ2dmO2aX;uJGF{e2G7 NVnCT3VVWmW4ZYLzZYwhd2ZiUD3ndE1u\WSrYYTl[EBufWy2aXTyeYchemW|aYP0ZY5k\SCjc4Pld5Nm\CCjczDlcohidmOnbXXueEBqdiCleYTveI95cWOrdImgc4Yh[WS{aXHtfYNqdg>? MUC4PVg1OTVz
C6 M1TaSGN6fG:2b4jpZ{BCe3OjeR?= MXuyMlUhdWdxbVy= M1jNflczKGh? NXz2Z4FPTE2VTx?= MlT5R5l1d3SxeHnjbZR6KGGpYXnud5QhemG2IFO2JINmdGy|IHH0JFUxKHWpL33MJJRwKDJwNTDt[{9uVA>? Ml;5PFk5QDZyNB?=
C6 NIjofIdHfW6ldHnvckBCe3OjeR?= NELrclQzNjVibXevcWw> MXe0JIg> Mk\GSG1UVw>? Ml;STY5pcWKrdHnvckBw\iC2dXL1cIlvKHCxbInt[ZJqgmG2aX;u M4fLPVg6QDh4MES=
C6 M3vP[GN6fG:2b4jpZ{BCe3OjeR?= M2jaUlIvPSCvZz;tUC=> MVq1JIg> MnHSSG1UVw>? NIS2TJBEgXSxdH;4bYNqfHliYYPz[ZN{\WRiYYOgZ4VtdCC{ZXzlZZNm M1z3PFg6QDh4MES=
SKVLB NGXo[3JEgXSxdH;4bYMhSXO|YYm= MXG3NkBp NF7jcHBKSzVyPU[uOVQh|ryP MWG5NFA{PTJy
MCF7/VP16 NXHVRVJFS3m2b4TvfIlkKEG|c3H5 MWm1NEDPxGdxbVy= M3vCN|czKGh? Mn7CTWM2OD1zND6yJO69VQ>? NU\2Sox1QTB{Mke5Ni=>
CA755 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PxNVExKM7:TR?= NXXyUY1yPiCm MU\JR|UxRTBwMEG0JO69VQ>? MUK5OVI3PTdy
CHO NGrQSHRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoDONVAh|ryP NF7oUIM3KGR? M3juPGlEPTB;MD6wOkDPxE1? NV;XeVFJQTV{NkW3NC=>
Panc02 MorkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFLkcJMyOCEQvF2= M4T0c|Yh\A>? NHj0WJdKSzVyPUCuNFY2KM7:TR?= Mm\kPVUzPjV5MB?=
PC14 NGXQdpBEgXSxdH;4bYMhSXO|YYm= MXy0JIQ> M1;NfGROW09? M1rn[GlEPTB;MD6yPUDPxE1? M4K4flk5OjJ3NEK=
HOP62 NYPiNmhzS3m2b4TvfIlkKEG|c3H5 NIrFW5I4OiCq NH[5fGtKSzVyPUCuNFA1KM7:TR?= NE\UUJk6QDd|Nk[x
SF-295 NGPOTW5EgXSxdH;4bYMhSXO|YYm= NHPVWpM4OiCq NGPEcoJKSzVyPUCuNFM2KM7:TR?= MmnrPVg4OzZ4MR?=
MDA-MB-361 MY\DfZRwfG:6aXOgRZN{[Xl? MXWxJIg> NH7HbnRFVVOR NGq1R5REgXSxdH;4bYNqfHliYXfhbY5{fCCPRFGtUWIuOzZzIHX4dJJme3OrbnegUIFkYiC5aYToJGlEPTBib3[gNE4xOTJizszN M{G3VVExOzl3NEmw
MDA-MB-361 MWLDfZRwfG:6aXOgRZN{[Xl? M2\a[|EhcA>? NYTzV|NWTE2VTx?= NIGxdYREgXSxdH;4bYNqfHliYXfhbY5{fCCPRFGgUWIhOzZzIHX4dJJme3OrbnegZ4Fz[m:6eYDldJRq\GG|ZTDzeGNRTzJid3n0bEBKSzVyIH;mJFAvODF6IN88US=> NX\lVYtMOTB|OUW0PVA>
CCRF-SB NH;2O3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGHL[3Y1KGR? NV\2dJFuTE2VTx?= M3i2OWlEPTB;MD6wNUDPxE1? NWHOUnF5OTB2MUG0O|Y>
CHO-K1 NGDiZZFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2HWZ|Qh\A>? MUnEUXNQ NELYRohKSzVyPUCuOEDPxE1? MnHGNVA1OTF2N{[=
KB NWTQemNlT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXG0JIQ> M{OyNWROW09? MmP2RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDkdpVoNXKnc3nzeIFvfCCNQkfEJJdqfGhiSVO1NEBw\iByLk[1JO69VQ>? Mlu3NVA1OTF2N{[=
KB NUi1VHFET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnTCOEBl NWfN[FBCTE2VTx?= MXrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuEQ3HtdJdqfGhiSVO1NEBw\iByLkC2JO69VQ>? MnW2NVA1OTF2N{[=
KB MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{nvN|Qh\A>? NWD2eXpuTE2VTx?= M3j4bGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[JJ2\y2{ZYPpd5RidnRiS1LNSHIhf2m2aDDJR|UxKG:oIECuOUDPxE1? M2[wOlExPDFzNEe2
KB MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXO0JIQ> MmXPSG1UVw>? MVTBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuEVkKwR{B4cXSqIFnDOVAhd2ZiMD60JO69VQ>? NWn5N3JMOTB2MUG0O|Y>
HTB-54 NHzaTFFEgXSxdH;4bYMhSXO|YYm= MUKxNFAh|ryP NX7mbm1jUUN3ME2xMlEh|ryP NVzBbWNROTB2N{[4OlE>
MEL-AC M{XEfGN6fG:2b4jpZ{BCe3OjeR?= NV;xOmVoOTByIN88US=> M4PM[2lEPTB;MT6yJO69VQ>? M1PaW|ExPDd4OE[x
DU145 MXvDfZRwfG:6aXOgRZN{[Xl? MW[3NkBp NG[5c41KSzVyPUCuNFUzQCEQvF2= MXmxNFQ4QTJ6Mh?=
K562R NWToVlFZS3m2b4TvfIlkKEG|c3H5 NXvWdllNPzJiaB?= M1zWO2lEPTB;MD6wNVU3KM7:TR?= MYqxNFQ4QTJ6Mh?=
PACA-2 NGPkTllEgXSxdH;4bYMhSXO|YYm= MVu2JIQ> NIfFcmZGTDVyPUCuNFA3OyEQvF2= M2W3WFExPDl6MkG0
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P388 NF\UcHVEgXSxdH;4bYMhSXO|YYm= MVe3NkBp MoDoS2k2OD1yLkCxNkDPxE1? NHzm[IMyODZ7MUexNC=>
HMV-1 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFzmTHZKSzVyPUCuNlgh|ryP MUCxNFY6QDR|Nh?=
MKN-1 NVmzSoJUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{nBbmlEPTB;MD62OEDPxE1? NGOyNVcyODZ7OESzOi=>
PC14 NEnZ[o9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmDOTWM2OD1zLkmg{txO MXmxNFY6QDR|Nh?=
T24 M4DTemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1XIU2lEPTB;MD62OEDPxE1? M3vSXFExPjl6NEO2
U937 M3uyXWZ2dmO2aX;uJGF{e2G7 NXTnU3VSOjRiaB?= M3foUmROW09? NVyycop6UW6qaXLpeJMhcW6lb4Lwc5JifGmxbjDv[kBcO0ifdHj5cYllcW6nIIfpeIghUUN3MDDv[kAxNjFizszN NFLuVHEyODd6MEmxNS=>
H460pv8/eto MmWxR5l1d3SxeHnjJGF{e2G7 MnfXNVAxKM7:TR?= Mm[zOFghcA>? NX\He3E{W2GuaX7l MnTCTWM2OD1yLkCyJO69VQ>? NHf6O5AyODd6MEmxNy=>
H460pv8 NFXBemdEgXSxdH;4bYMhSXO|YYm= MWexNFAh|ryP NXvibVFRPDhiaB?= MlO4V4FtcW6n NHnh[3NKSzVyPUCuNFA1KM7:TR?= MmL1NVA4QDB7MUO=
MDA-231 MXnDfZRwfG:6aXOgRZN{[Xl? MnrkNVAxKM7:TR?= NI\rWmY1QCCq NV\nT5UxW2GuaX7l MlXkR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDLUKzNUBk\WyuIDjFVk0tTUeIUjuscZV1[W62IIC1N{khf2m2aDDJR|UxKG:oIECuNFA3KM7:TR?= MUCxNFc5ODlzMx?=
MDA-468 NF;tVGtEgXSxdH;4bYMhSXO|YYm= NF22V5kyODBizszN M3XYbVQ5KGh? M4PJWnNidGmwZR?= M4X5dGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQT20Olgh[2WubDCoSXIuNCCjbYDsbYZq\WRiRVfGVkwhdXW2YX70JJA2Oylid3n0bEBKSzVyIH;mJFAvODB3IN88US=> MVexNFc5ODlzMx?=
NCI-H226 MmjYR5l1d3SxeHnjJGF{e2G7 NIj1eGoyODBizszN NF\hWoI1QCCq MmHrV4FtcW6n NYnrSnRGS3m2b4TvfIlkcXS7IHHnZYlve3RiTlPJMWgzOjZiY3XscEApdXW2YX70JJA2Oylid3n0bEBKSzVyIH;mJFAvODB6IN88US=> MXWxNFc5ODlzMx?=
NCI-H322 MnTNR5l1d3SxeHnjJGF{e2G7 MV2xNFAh|ryP Mn3YOFghcA>? M3HyR3NidGmwZR?= NF\iXmNEgXSxdH;4bYNqfHliYXfhbY5{fCCQQ1mtTFMzOiClZXzsJEhufXSjboSgdFU{MSC5aYToJGlEPTBib3[gNE4xOyEQvF2= NFrnc|EyODd6MEmxNy=>
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SKBR-3 M2fLXmN6fG:2b4jpZ{BCe3OjeR?= NVvHT3pWOTByIN88US=> M3zjUVQ5KGh? M4\G[nNidGmwZR?= NILnNYZEgXSxdH;4bYNqfHliYXfhbY5{fCCVS1LSMVMh[2WubDCoSXIu[W2ybHnmbYVlKGW{QkKscZV1[W62IIC1N{khf2m2aDDJR|UxKG:oIECuNFIh|ryP M4X2WFExPzhyOUGz
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ZR-75-1 MXLDfZRwfG:6aXOgRZN{[Xl? NXXJc5NIOTByIN88US=> Mnj2OFghcA>? M1PUVXNidGmwZR?= MXPDfZRwfG:6aXPpeJkh[WejaX7zeEBbWi15NT2xJINmdGxiKFXSL{xx\3JtLH31eIFvfCCyNUOpJJdqfGhiSVO1NEBw\iByLkCwPUDPxE1? NWP3[JZNOTB5OEC5NVM>
L1210 MojyR5l1d3SxeHnjJGF{e2G7 MWfJR|UxRTBwMEK1JO69VQ>? NX3udmFVOTB6OUCxOlc>
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OVCAR-3 MVPDfZRwfG:6aXOgRZN{[Xl? MkCwNlQhcA>? NHP5WGlFVVOR MnXlTWM2OD1yLkCzOUDPxE1? M1XWW|ExQTV4MkG0
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H4 MYjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{P3RWlEPTB;N{GuOVYhdk1? MoD4V2FPT0WU
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HCC1599 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4PIc2lEPTB;N{KuOVQhdk1? MVnTRW5ITVJ?
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Calu-1 MX7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHzQcFlKSzVyPUezMlYhdk1? NHnPSYxUSU6JRWK=
DU-145 NWXkTFg3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH23eHhKSzVyPUezMlc{KG6P MV7TRW5ITVJ?
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HSC-4 MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2PDT2lEPTB;N{WuO|Yhdk1? M{LSUHNCVkeHUh?=
NH-12 NH7Ye4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYDXZVdNUUN3ME23OkBvVQ>? NXX4N|J5W0GQR1XS
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TUR M3vq[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M16zeWlEPTB;N{[uN|Ihdk1? M2PienNCVkeHUh?=
TE-1 NX\uZWJNT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7UdZBVUUN3ME23Ok4{PSCwTR?= NHPaRppUSU6JRWK=
CMK MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnztTWM2OD15Nz60NkBvVQ>? MmrHV2FPT0WU
LB996-RCC MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlXwTWM2OD15OD60OUBvVQ>? MojtV2FPT0WU
NCI-H1650 MnTZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYXTb2VRUUN3ME23PE43OiCwTR?= NGfLcFJUSU6JRWK=
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NOS-1 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYfJR|UxRTd7LkG0JI5O M1PoTnNCVkeHUh?=
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A172 M1jXXGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MW\JR|UxRThyLkW1JI5O MoX4V2FPT0WU
TE-12 MkDnS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmPFTWM2OD16MT6wOkBvVQ>? MkfzV2FPT0WU
MKN45 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX7JUIN1UUN3ME24NU42OSCwTR?= NGS5dnpUSU6JRWK=
LoVo MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2XscGlEPTB;OEGuPFIhdk1? MnnpV2FPT0WU
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ETK-1 NFXkVoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{H3cWlEPTB;OEOuNFEhdk1? M{\IV3NCVkeHUh?=
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KM-H2 Mle0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NI\hS2FKSzVyPUmwMlY5KG6P MoPFV2FPT0WU
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SF268 M3i4V2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2PqWmlEPTB;OUKuOFIhdk1? NHL6OohUSU6JRWK=
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SW1783 MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXLCO5BFUUN3ME25O{46QSCwTR?= NXHnXmpZW0GQR1XS
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G-402 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWfRTZVUUUN3ME25PU4xQSCwTR?= NETP[FBUSU6JRWK=
MES-SA NF7rR3dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTl7LkWzJI5O M3z0XHNCVkeHUh?=
FADU M2LpRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEHXSWNKSzVyPUGwNk4yQCCwTR?= NWDJV2c2W0GQR1XS
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NCI-H1618 NHjRN25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX\JR|UxRTFyND6zOUBvVQ>? NXHoRppDW0GQR1XS
AU565 M1ywcGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF7TU|NKSzVyPUGwOE42OSCwTR?= NEHUfZpUSU6JRWK=
SF126 NFfqO21Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlrlTWM2OD1zMESuOVghdk1? MVzTRW5ITVJ?
MMAC-SF NFfBS4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MorJTWM2OD1zME[uOFghdk1? NELXbnhUSU6JRWK=
ChaGo-K-1 NH;CNG1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYP1OXk2UUN3ME2xNFcvQTVibl2= MXjTRW5ITVJ?
NCI-H596 NFrPNHBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXnqbpY3UUN3ME2xNFgvODFibl2= NHLXN25USU6JRWK=
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HCC1569 MoLES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXLJR|UxRTFyOT6zOEBvVQ>? M2XuZ3NCVkeHUh?=
NCI-H2170 NHnDNW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{nyZ2lEPTB;MUC5MlkzKG6P MnvNV2FPT0WU
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BOKU M4n4R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVjnNnVRUUN3ME2xNVAvQDdibl2= NVOzTG1sW0GQR1XS
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SW626 MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M33zTWlEPTB;MUGxMlcyKG6P M1XEO3NCVkeHUh?=
EHEB M4jEVWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlfWTWM2OD1zMUKuOVkhdk1? MWLTRW5ITVJ?
TE-10 M2TPNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MV\JR|UxRTFzMj62PEBvVQ>? MluwV2FPT0WU
MZ7-mel MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MknsTWM2OD1zMUKuPFQhdk1? MXHTRW5ITVJ?
SAS MYXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYLEblRbUUN3ME2xNVQvPDVibl2= MWfTRW5ITVJ?
TK10 Mm\0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGnvdYRKSzVyPUGxOE43KG6P NV3VV3ZlW0GQR1XS
SW13 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\TTWM2OD1zMUWuNlYhdk1? NXnkbHRsW0GQR1XS
HCC1937 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX\JR|UxRTFzNT64OEBvVQ>? NUHPSpBbW0GQR1XS
SK-NEP-1 M1\qfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH3Ne5lKSzVyPUGxOk4zPiCwTR?= MlfvV2FPT0WU
OVCAR-5 MnfDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;4OGxKSzVyPUGxOk45OSCwTR?= NFP0T2pUSU6JRWK=
SW837 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mk\VTWM2OD1zMUeuPVUhdk1? NUTqPW4xW0GQR1XS
BFTC-909 MmfYS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYHJR|UxRTFzOD60PEBvVQ>? MWfTRW5ITVJ?
SW872 NHG3UnhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NY\yVWVuUUN3ME2xNlAvQDFibl2= NWXZV4VYW0GQR1XS
SF539 NXLrVphJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWHHdYU{UUN3ME2xNlEvPzJibl2= NGi5UpZUSU6JRWK=
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LB647-SCLC NX\nbm92T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXfJR|UxRTF{Mz6zOUBvVQ>? NFrCbXRUSU6JRWK=
NCI-H1703 NYnHW|FiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmXOTWM2OD1zMk[uOFIhdk1? NH34[JVUSU6JRWK=
KURAMOCHI NGWyXJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUHJR|UxRTF{Nj63OkBvVQ>? MWHTRW5ITVJ?
ATN-1 NWHLWI4{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF\4ZpJKSzVyPUGyO{44KG6P NFXL[WdUSU6JRWK=
SK-CO-1 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmXYTWM2OD1zMkmuNlEhdk1? NYf5VYxCW0GQR1XS
RPMI-7951 M3vndGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVLJR|UxRTF|MD6xOEBvVQ>? Ml[xV2FPT0WU
NCI-H1648 M{LpSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkH5TWM2OD1zM{GuPVQhdk1? MXjTRW5ITVJ?
NCI-H64 M2r1VGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjNZYFoUUN3ME2xN|IvPjVibl2= NWL5TItSW0GQR1XS
HT-3 NVfRXGluT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWrJR|UxRTF|Mz6wOEBvVQ>? MlLQV2FPT0WU
NCI-H1770 M2HhfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmDNTWM2OD1zM{OuNlEhdk1? MX3TRW5ITVJ?
NB13 NEHwV25Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF\Sc5RKSzVyPUGzOE42PSCwTR?= M4rYU3NCVkeHUh?=
NCI-H747 MkTqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MW\JR|UxRTF|NT6wNkBvVQ>? MYLTRW5ITVJ?
ML-2 M{W5Nmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXvJR|UxRTF|NT6xOkBvVQ>? NGjjSHVUSU6JRWK=
CA46 NFXFOGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUDPUYhuUUN3ME2xN|YvQDhibl2= M4DhZXNCVkeHUh?=
ES6 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfnO2tNUUN3ME2xN|cvOTJibl2= MUXTRW5ITVJ?
JiyoyeP-2003 NGToVo9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYfsTWNtUUN3ME2xN|cvQCCwTR?= NYLVfXY2W0GQR1XS
NMC-G1 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnXsTWM2OD1zM{muNVMhdk1? M2TKRnNCVkeHUh?=
U-698-M MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUHBe|VkUUN3ME2xN|kvPSCwTR?= M2e4bnNCVkeHUh?=
LU-99A MnriS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;GTmU1UUN3ME2xOFYvPTVibl2= M{PsS3NCVkeHUh?=
NCI-H748 NUTpUYJvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4nZN2lEPTB;MUS3MlMyKG6P NHzvRVRUSU6JRWK=
SK-MEL-3 M4fheWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHG2b4tKSzVyPUG0O{43OSCwTR?= MXLTRW5ITVJ?
SNB75 MnvrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonuTWM2OD1zNEiuOlUhdk1? NXqwXno3W0GQR1XS
BB65-RCC MkPtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3Owe2lEPTB;MUS5MlMyKG6P NV7scoRVW0GQR1XS
NCI-H1355 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2P5cGlEPTB;MUWyMlI1KG6P NHfmdmlUSU6JRWK=
SCC-25 MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPlPGNFUUN3ME2xOVMvPzNibl2= M3LOfnNCVkeHUh?=
OAW-42 Mk\LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTF3Mz64OEBvVQ>? MkPKV2FPT0WU
MONO-MAC-6 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHHY[W9KSzVyPUG1OE4{PiCwTR?= MmDxV2FPT0WU
WSU-NHL Ml:zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXP3bYY3UUN3ME2xOVQvPThibl2= NHLzWZZUSU6JRWK=
SNU-423 Mk[xS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3fucGlEPTB;MUW1MlM1KG6P MnO3V2FPT0WU
SBC-1 NFS2WYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYj2cpBUUUN3ME2xOVYvPCCwTR?= M4LUSnNCVkeHUh?=
OVCAR-4 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTF3Nj63PEBvVQ>? MUjTRW5ITVJ?
NCI-H2347 NUPibVVOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVi4VmIxUUN3ME2xOVgvOzRibl2= NFS1Z2FUSU6JRWK=
CAMA-1 MkiwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVjJXYNiUUN3ME2xOVkvOzNibl2= NGCyZ3NUSU6JRWK=
GDM-1 NHjscXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGricGdKSzVyPUG1PU44KG6P M4OwXXNCVkeHUh?=
KNS-81-FD M1HPdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{jJV2lEPTB;MU[wMlMzKG6P M3fCdnNCVkeHUh?=
no-10 M3PKT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfJcGkyUUN3ME2xOlAvOzhibl2= NFfkcm9USU6JRWK=
NCI-H2052 NULMVVJwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3rRWWlEPTB;MU[wMlQ4KG6P M2nPeXNCVkeHUh?=
DB NX7YR5JvT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYDJR|UxRTF4MT63NUBvVQ>? NW[0cI5UW0GQR1XS
A4-Fuk MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWPJR|UxRTF4Mj60PEBvVQ>? NUXMT5JiW0GQR1XS
HCE-T Ml70S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH74[ohKSzVyPUG2OE45PyCwTR?= MkfqV2FPT0WU
HuP-T3 NHz0N2dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{DNeGlEPTB;MU[1MlU2KG6P MoHGV2FPT0WU
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A704 NEm2SJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVPJR|UxRTJwMkmxN|Uh|ryP M3XCO3NCVkeHUh?=
EVSA-T NHfNc4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\UTWM2OD1{LkOwNFg6KM7:TR?= MkDkV2FPT0WU
HCT-15 MkC4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXXnVmlmUUN3ME2yMlM2PDZ4IN88US=> NUXiNFZ{W0GQR1XS
HuO-3N1 MmfiS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGTVd5RKSzVyPUKuN|Y2QDNizszN Mn76V2FPT0WU
GMS-10 MlXBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVzQN5hHUUN3ME2yMlM6PzN6IN88US=> MXnTRW5ITVJ?
TGBC24TKB NXX4bIF{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NI\GbnFKSzVyPUKuOFE1OjlizszN NFzLeolUSU6JRWK=
C2BBe1 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLYUHFKSzVyPUKuOVE{OjhizszN NEC5XVlUSU6JRWK=
SK-N-DZ NUnnfXRxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{S5dmlEPTB;Mj61OlU6OiEQvF2= NFfRZ3JUSU6JRWK=
SK-N-AS NW\Ub2FHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M37VOmlEPTB;Mj63N|AyPSEQvF2= NYXaS3ZmW0GQR1XS
T98G NGiwdI1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWnvbopnUUN3ME2yMlgxPDBzIN88US=> MkDUV2FPT0WU
DMS-79 MmfQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoLkTWM2OD1{LkmwPVg5KM7:TR?= MmLsV2FPT0WU
SNU-475 NGn2fYNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTJwOUK1O|Ih|ryP MmnZV2FPT0WU
COLO-792 NUS0cVRUT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4DyXWlEPTB;Mz6wNlg4KM7:TR?= MX\TRW5ITVJ?
YAPC M{[y[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfRTWM2OD1|LkOyPFMzKM7:TR?= NVjiPWl5W0GQR1XS
UACC-812 Ml3lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVezO2plUUN3ME2zMlM{PzN6IN88US=> NYSzWnhPW0GQR1XS
Mewo MXLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{f4fmlEPTB;Mz60OlA5PiEQvF2= MonEV2FPT0WU
COLO-824 NV7MfVhrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MojFTWM2OD1|LkW3N|c5KM7:TR?= NGXrZZRUSU6JRWK=
SCC-9 MoflS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFLWS2ZKSzVyPUOuPVE{ODlizszN M1r0eHNCVkeHUh?=
CP50-MEL-B NWDUTJp2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4[1NmlEPTB;Mz65PFcxOyEQvF2= NYfCS5dWW0GQR1XS
NCI-H345 NFXqfZpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWrhe5hrUUN3ME20MlAyOTd2IN88US=> NFrkbotUSU6JRWK=
COR-L88 M1XzNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVnJR|UxRTRwM{m1PEDPxE1? NXLlW4ZCW0GQR1XS
RCM-1 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXnyTIFuUUN3ME20MlQ6Ojd{IN88US=> MVzTRW5ITVJ?
JVM-3 MlmxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2fkeGlEPTB;ND62NFYyQCEQvF2= MmraV2FPT0WU
ECC4 NGHsZY9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1TZVGlEPTB;ND63OlU1OSEQvF2= M{nmTnNCVkeHUh?=
MDA-MB-453 MnfXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3LPOGlEPTB;ND64OVE{PCEQvF2= NHnacIRUSU6JRWK=
SK-N-FI NW\2THp6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXHiWHBWUUN3ME21MlIzQDd5IN88US=> NYG2N2RDW0GQR1XS
D-502MG NHq4bXFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4rQZ2lEPTB;NT6yOFI3OiEQvF2= NIXtfoFUSU6JRWK=
NCI-H2227 NXn0fpV6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoTpTWM2OD13LkO2NlMyKM7:TR?= MnTnV2FPT0WU
DMS-53 NWS0WXJGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYHmUZI6UUN3ME22MlE4OTB4IN88US=> MXLTRW5ITVJ?
LB771-HNC MorlS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXPV[mFxUUN3ME22MlQ3QDB4IN88US=> NUj4T|JiW0GQR1XS
BB49-HNC NFrCT2NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHzZS|JKSzVyPUeuOFE4QTFizszN M3XRc3NCVkeHUh?=
MDA-MB-415 MlPCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYfJR|UxRTdwNUezNFMh|ryP MXXTRW5ITVJ?
TALL-1 NEPv[JFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1XQN2lEPTB;Nz65Olg5KM7:TR?= M4nKS3NCVkeHUh?=
HCC1395 NXf1NpN3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NF3k[JFKSzVyPUiuNVUyOjJizszN M1HucXNCVkeHUh?=
EFE-184 NUDOOnlsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXrENGJ2UUN3ME24MlM4OjF4IN88US=> NVHkOVBXW0GQR1XS
EC-GI-10 NWXheZVmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1;iXWlEPTB;OD62PFY5PiEQvF2= NVzGNoNRW0GQR1XS
TT M1fi[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVi2N|NNUUN3ME25MlA3QTZ4IN88US=> MYfTRW5ITVJ?
U-2-OS NUDyd2lYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWjJR|UxRTlwOEGzPVMh|ryP M3LVVHNCVkeHUh?=
SHP-77 NYLWe5FQT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MYrJR|UxRTlwOUCyPUDPxE1? MnL6V2FPT0WU
HCC1143 NFjRTlJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVjJR|UxRTFyLkS3PVch|ryP NWHvbWpSW0GQR1XS
A498 Mnm1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoHGTWM2OD1zOD6yPVc3KM7:TR?= MnX0V2FPT0WU
LS-513 Ml\KS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVHJR|UxRTF6Lk[wPVMh|ryP M1\wUHNCVkeHUh?=
K052 Ml7hS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHnCbY5KSzVyPUKxMlQ1OThizszN M{jIeXNCVkeHUh?=
SiHa M{nXT2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXnJR|UxRTJ|LkOzO|Eh|ryP NYTnTm9JW0GQR1XS
RCC10RGB MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1nXeWlEPTB;M{CuNFYyOSEQvF2= M3P2UHNCVkeHUh?=
NCI-SNU-16 NVXkTGFFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFPDVGVKSzVyPUOxMlE5OzZizszN M1j2VHNCVkeHUh?=
SNU-C1 NED3VpNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkTiTWM2OD1|Mj60NVc2KM7:TR?= NV;PR5hNW0GQR1XS
C32 MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MnLWTWM2OD1|Mj64O|Ih|ryP MWrTRW5ITVJ?
SW684 M3zNfGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUnJR|UxRTN2LkG1OlEh|ryP NI\KXJhUSU6JRWK=
SK-MEL-28 M33a[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUTJR|UxRTN3LkWzO|ch|ryP MWXTRW5ITVJ?
SK-MEL-1 M2PsNWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUnjUnM{UUN3ME2zOk44PTR5IN88US=> NGC3ZVFUSU6JRWK=
NCI-H720 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYXsSWZwUUN3ME20NU46PzJ4IN88US=> M1TMVXNCVkeHUh?=
P31-FUJ MlPXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mor1TWM2OD12ND6zNlg1KM7:TR?= M3z2N3NCVkeHUh?=
NCI-H524 MmC3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGHEXIFKSzVyPUS4MlgzPDhizszN NF\ESHZUSU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
POU5F1 / SOX2 / NANOG; 

PubMed: 27929731     


NT2/D1 cells treated with 10 nM of doxorubicin were subjected to WB analysis for pluripotency factors (POU5F1, NANOG, SOX2) and differentiation markers (TUBB3).

ATG5 / ATG7 / SQSTM1 / LC3A-II / LC3B-II / p-mTOR; 

PubMed: 27929731     


NT2/D1 cells treated with 10 nM of doxorubicin were subjected to WB analysis for autophagy markers (ATG5, ATG7, SQSTM1, LC3A-II, LC3B-II and p-MTOR). 

P62 / LC3II; 

PubMed: 28208617     


(A,B) Doxorubicin induces autophagy in pancreatic cancer cells. Western blot analysis of LC3-II and p62 protein levels in Mia PaCa-2 and Panc-1 cell lines treated with indicated concentrations of doxorubicin for 24 h, or treated with 2.5 μM doxorubicin for indicated times.

p-AKT(T308) / p-Akt(S473) / Akt / p-PRAS40(T246) / PRAS40 / p-H2A.X(S139); 

PubMed: 26027929     


MCF10A cells were serum-starved and treated with (A) 2 μM doxorubicin over a 10 hour time course.

27929731 28208617 26027929
Growth inhibition assay
Cell viability; 

PubMed: 25871400     


Salinomycin enhances the cytotoxicity of doxorubicin in HCC cellsRelative cell viability (mean ± SD) for salinomycin (black), doxorubicin (blue) and doxorubicin plus salinomycin (red) in HuH-7, HepG2, SNU-449 and SNU-387 cells.

25871400
Immunofluorescence
LC3; 

PubMed: 24970805     


Doxorubicin induces GFP-LC3 puncta formation in hepatocellular carcinoma cells. SK-Hep1 cells were treated with doxorubicin (2.5 μM) or CQ (10 μM) plus doxorubicin for 7 h and cells were examined with a confocal microscope for GFP-LC3 punctuation/aggregation.

24970805
ELISA
OPN; 

PubMed: 24963635     


A549 were exposed to 50 nM doxorubicin or PBS for 48 h. OPN concentrations in cell culture media were determined by ELISA.

24963635
In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (172.41 mM)
Water 20 mg/mL (34.48 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl

CAS No. 25316-40-9
Storage powder
in solvent
Synonyms NSC 123127

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

  • Mass
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    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03571321 Not yet recruiting Acute Lymphoblastic Leukemia|ALL Childhood|ALL University of Chicago|Incyte Corporation September 5 2019 Phase 1
NCT03571321 Not yet recruiting Acute Lymphoblastic Leukemia|ALL Childhood|ALL University of Chicago|Incyte Corporation September 5 2019 Phase 1
NCT03108300 Not yet recruiting Malignant Soft Tissue Sarcoma Ain Shams University August 30 2019 Phase 2
NCT03108300 Not yet recruiting Malignant Soft Tissue Sarcoma Ain Shams University August 30 2019 Phase 2
NCT03437070 Withdrawn Leiomyosarcoma University of Miami|Janssen Scientific Affairs LLC June 2019 Phase 1
NCT03349281 Recruiting Refractory Acute Lymphoblastic Leukemia|Relapsed Acute Lymphoblastic Leukemia Julio Barredo|Takeda|University of Miami June 2019 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Why the IC50 of the compound varied over time in our measurement?

  • Answer:

    Sometimes the IC50 value might vary between different batch of compounds due to the slight differences in purity. If different IC50 was seen in the same batch of compound, the problem may lies in the process of dissolvement. Make sure that you see no particles and the solution is clear with your stock solution.

Topoisomerase Signaling Pathway Map

Related Topoisomerase Products0

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID