Doxorubicin (Adriamycin) HCl

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127

Doxorubicin (Adriamycin) HCl Chemical Structure

Molecular Weight(MW): 579.98

Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.

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Cited by 36 Publications

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  • Enhancement of apoptotic response by addition of targeted agents lapatinib and sunitinib to doxorubicin in the same device reservoir at 24 hours. P values were determined using Student’s t test for all graphs.

    Sci Transl Med, 2015, 7(284): 284ra57 . Doxorubicin (Adriamycin) HCl purchased from Selleck.

    (F) Kaplan-Meier curves are shown for all groups described: no hydrogel, a hydrogel loaded with paclitaxel, or a hydrogel loaded with doxorubicin. The number of mice per group (n) and median survival (ms) are listed. The experiment was performed at least three times.

    Science, 2018, 10(433), doi: 10.1126/scitranslmed.aar1916. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • MM.1S cells inducibly expressing shRNA targeting SIRT6 were grown with or without doxycycline, and then treated with vehicle, doxorubicin (Doxo; 1 μM) or melphalan (mel; 100 μM) for 1 hour prior to the preparation of lysates and analyzed by western blotting. Representative immunoblots (n = 3) for the indicated proteins in MM cells are shown.

    Blood, 2016, 127(9):1138-50. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Cell viabilities with increasing concentrations of cisplatin (CP) and doxorubicin (DOXO) under normoxic and hypoxic condition for 48 hours were determined by MTT assay. IC50 values are presented as the means ?SDs (n=4) and * denotes p<0.05.

    Cancer Res 2014 74, 298-308. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • Cleavage of caspase-3, -9 and the expression of PARP were measured by western blot using b-actin as a loading control.

    Cell Cycle, 2017, 16(5):468-476. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    (C) & (D) Treatments with doxorubicin, cisplatin or AZD6244 further decreased the cell viability in RH30 cloned cells in which STAT3 or GP130 was knockdown by shRNA. (*P<.05, ** P <.01, *** P <.001).

    Curr Cancer Drug Targets, 2016, 16(7):631-8. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  •  

    Doxorubicin release of P(MAA-g-EG) and P(MAA-g-EG) containing nanoparticles in two-step pH conditions. Doxorubicinloaded P(MAA-g-EG) ,P(MAA-g-EG)-1.0NP ,P(MAA-g-EG)-2.5NP, and P(MAA-g-EG)-5.0NP crushed particles (75–150 um) were released in 1 PBS (pH 2.0) for 90 min. Then the pH was increased to 7.0 by adding 5N NaOH and release continued for 6 h.

    J Biomed Mater Res Part A 2012 10.1002/jbm.a.34064. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Temporal uptake of free DOX or DOX-PCL63-b-PNVP90 micelles into K-562 (I) and DL (J) cells were studied by incubating in 24-well plates at a concentration of 50,000 cells per well. Representative images shown were obtained using a fluorescence microscope Eclipse 80i (Nikon, Japan) (Plan Fluor, 40X, NA 0.75 objective) equipped with blue and red filters for, Hoechst and Doxorubicin respectively (n = 3).

    PLoS One 2014 9, e94309. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • PLoS One 2013 8(1), e54595. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    CON+ -positive control (with FBS as chemoattractant); CON- - negative control (without chemoattractant); DOX -doxorubicin; WZ -WZ811; PF- PF-573228; * -significant difference compared to positive control: p<0.05; ** -significant difference compared to positive control: p<0.01; # -significant difference between combination and inhibitor only: p<0.05. Cancer cell invasion was analyzed using Transwell membranes assembled in 24-well plates and covered with a layer of Matrigel. COR-L23 cells were treated for 24 h with 50 nM DOX, 10 uM WZ811, 5 uM PF-573228 and combinations of inhibitors with 50 nM DOX. Similarly, RH460 cells were treated for 24 h with 1 uM DOX, 10 uM WZ811, 2.5 uM PF-573228 and combinations of inhibitors with 1 uM DOX. Applied doses were the ones that exerted the most pronounced synergistic effect on cytotoxicity level. After the incubation period, cells that invaded through matrigel and membranes were fixed in 4% paraformaldehyde-PBS and stained with Hoechst 33342 nuclear stain. Cells were visualized and counted under a fluorescent microscope at 10x magnification.

    2014 Dr Milica Pesic from Institute for Biological Research. Doxorubicin (Adriamycin) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.
Targets
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]
Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
L1210 MoHHSpVv[3Srb36gRZN{[Xl? NGTwfWFKdmirYnn0bY9vKG:oIGLORUB{gW62aHXzbZMhf2m2aDDFSFUxKG:oIECuOVgh|ryP M4e3[FQ6ODV|Nh?=
Yoshida sarcoma NFjPRoJHfW6ldHnvckBCe3OjeR?= NWnTUZVoUW6qaXLpeIlwdiCxZjDbN2heXGSUIHnuZ49zeG:{YYTpc44hf2m2aDDJR|UxKG:oIECuNFIh|ryP MojZNVMzOjl6Nh?=
CHO MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTBwME[g{txO NXS1O4E5OTN3NEe1NC=>
8226 NFrtTnRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF;h[5NKSzVyPUCuNFM{KM7:TR?= MXSxOFQ4PzNy
8226/ADR NVvGdYdMT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NXvrRYYxUUN3ME2wMlU3KM7:TR?= M3nUSlE1PDd5M{C=
MCF7/ADR Ml;QS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWHSXXdIUUN3ME2zMlEh|ryP Ml\rNVQ1Pzd|MB?=
H69 NIjUOmlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYPtZnNHUUN3ME2wMlg4KM7:TR?= MoLtNVQ4QTV6NR?=
N417 NYnKdWhjT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUO1OJJCUUN3ME2wMlI{KM7:TR?= NUjVd5FLOTR5OUW4OS=>
H9 Ml70S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NX7yZ5d7UW6qaXLpeIlwdiCxZjDIPUBk\WyuczDjbJJwdmmlYXzsfUBqdm[nY4Tl[EB4cXSqIITo[UBJUVZvMTCoTFkwUUmLQjmge4l1cCCLREWwJI9nKDBwMEGg{txO NFvMclQyPTR6Nkix
T-C8166 M{P1c2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYXJSFUxRTBwMEGg{txO MkHYNVU1QDZ6MR?=
T-H9 NGPTSmFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2f0NmlFPTB;MD6wNUDPxE1? NUTZd5VQOTV2OE[4NS=>
T-MT4 MVHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWS2XoZsUUR3ME2wMlAyKM7:TR?= NFrjU4EyPTR6Nkix
SKMEL-5 NYnmR3RIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LpN2VFPTB;Mz6yJO69VQ>? NV\xTnB2OTZzM{e1Ny=>
MLM MWPDfZRwfG:6aXOgRZN{[Xl? NWLLVpJTTUR3ME2wMlE{KM7:TR?= NXq3b4NrOTh5NUO1NC=>
KBM-3 NYm4O3E4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVfsXWRlUUN3ME2wMlAzPiEQvF2= NYTxfZBHOTl7NUi3Oy=>
HT-29 M3Xxb2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWj1eXI3OC5yMTFOwIc> NXPScnpmPDhiaB?= NVLrUIFLSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJk> MnTKNlc4QDR2OR?=
EMT6 M3zPfmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1vHcVAvPSEQvF2= NHnT[nIyKGh? MULJcohq[mm2aX;uJI9nKHC{b3zp[oVz[XSrb36gZpkhdWWjc4XybY5oKG[xcjDzeZJ3cX[rbneg[pJi[3Srb37zJJdqfGhiRVS1NEBw\iByLkCwNFEh|ryP MX:3OFUzPjd2
H2981 NEnHV3VEgXSxdH;4bYMhSXO|YYm= M2Dkb2lEPTB;MD61JO69VQ>? NVHBdYZsPzd|MUCyNy=>
LOX MXHDfZRwfG:6aXOgRZN{[Xl? M3exSGlEPTB;MD6wOFIh|ryP NHPmSIw4QDV|M{Ox
C38 M1fzT2N6fG:2b4jpZ{BCe3OjeR?= MVzJR|UxRTBwMEGg{txO MkDyPFA2PzJ7MR?=
EAC NULhRZBuS3m2b4TvfIlkKEG|c3H5 NVL3OokzPzJiaB?= Mo\2TWM2OD1yLkO1JO69VQ>? NITYVlc5OzVyMEi3
SKVLB MnTVR5l1d3SxeHnjJGF{e2G7 NID5U3lKSzVyPUWuOkDPxE1? NYTWWG1rQDV5NkmxOC=>
AUC375 NUCxXZVRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTBwMUGyJO69VQ>? NYHI[2ppQDZ2OE[wNC=>
A2780-C25 NUnpdWNXS3m2b4TvfIlkKEG|c3H5 NITzSoZEgXSxdH;4bYNqfHliYXfhbY5{fCCxeHHsbZBt[XSrbj3y[ZNqe3SjboSgRVI4QDBvQ{K1JJdqfGhiSVO1NEBw\iByLkC2N{DPxE1? MlTmPFg{OTd3NR?=
A2780-CP3 MnL0R5l1d3SxeHnjJGF{e2G7 NF;O[29EgXSxdH;4bYNqfHliYXfhbY5{fCClaYPwcIF1cW5vcnXzbZN1[W62IFGyO|gxNUOSMzD3bZRpKEmFNUCgc4YhOC5yNU[g{txO MmTtPFg{OTd3NR?=
A2780-DX5 MV7DfZRwfG:6aXOgRZN{[Xl? NH\EeZpEgXSxdH;4bYNqfHliYXfhbY5{fCCmb4jvdpVjcWOrbj3y[ZNqe3SjboSgRVI4QDBvRGi1JJdqfGhiSVO1NEBw\iByLkO1O{DPxE1? M4PZclg5OzF5NUW=
MXF7 NX;FfGo5S3m2b4TvfIlkKEG|c3H5 MoC3RY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4Sgd4Vve2m2aY\lJG1ZTjdid3n0bEBKSzVyIH;mJFAvODJ6IN88US=> MXi4PVYxPTV6
MXF7 NELue3hEgXSxdH;4bYMhSXO|YYm= NFvLWHBCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBucXSxeHHueJJwdmVvcnXzbZN1[W62IF3YSlchf2m2aDDJR|UxKG:oIECuNFI5KM7:TR?= NYHIRWV5QDl4MEW1PC=>
MXF7 NVziZlRMS3m2b4TvfIlkKEG|c3H5 MWrBcpRqfHWvb4KgZYN1cX[rdImgZYdicW6|dDDkc5hwenWkaXPpck1z\XOrc4ThcpQhVViINzD3bZRpKEmFNUCgc4YhOS5zN{Kg{txO NUi3PYhsQDl4MEW1PC=>
WiDr NF3pO4VEgXSxdH;4bYMhSXO|YYm= NIDpPXdC[3Srdnn0fUBi\2GrboP0JJNmdnOrdHn2[UBYcUS{IHPlcIwhf2m2aDDJR|UxKG:oIECuNFU{KM7:TR?= NWrFfHJvQDl4MEW1PC=>
MCF M13Mc2Z2dmO2aX;uJGF{e2G7 NYjITIY6WmW4ZYLzZYwhd2ZiUD3ndE1u\WSrYYTl[EBufWy2aXTyeYchemW|aYP0ZY5k\SCjc4Pld5Nm\CCjczDlcohidmOnbXXueEBqdiCleYTveI95cWOrdImgc4Yh[WS{aXHtfYNqdg>? MXm4PVg1OTVz
C6 MYfDfZRwfG:6aXOgRZN{[Xl? NGHoXlEzNjVibXevcWw> NUjUTWxQPzJiaB?= MX7EUXNQ MnnCR5l1d3SxeHnjbZR6KGGpYXnud5QhemG2IFO2JINmdGy|IHH0JFUxKHWpL33MJJRwKDJwNTDt[{9uVA>? MlXrPFk5QDZyNB?=
C6 M2jWeWZ2dmO2aX;uJGF{e2G7 NYjFd3diOi53IH3nM41N NGK2OYo1KGh? NX7lXWF{TE2VTx?= NHzpdG9KdmirYnn0bY9vKG:oIIT1ZpVtcW5icH;sfY1memm8YYTpc44> NGPNN4c5QTh6NkC0
C6 NXvXUYlOS3m2b4TvfIlkKEG|c3H5 NXXMdI1XOi53IH3nM41N NY[xZ3ROPSCq NEK4dJlFVVOR MoraR5l1d3SxeHnjbZR6KGG|c3Xzd4VlKGG|IHPlcIwhemWuZXHz[S=> MnXEPFk5QDZyNB?=
SKVLB MkTmR5l1d3SxeHnjJGF{e2G7 NHvDdFk4OiCq NYj6N5d{UUN3ME22MlU1KM7:TR?= MmfTPVAxOzV{MB?=
MCF7/VP16 MXfDfZRwfG:6aXOgRZN{[Xl? MYS1NEDPxGdxbVy= NXizZWg{PzJiaB?= NX31WWhjUUN3ME2xOE4zKM7:TR?= MVe5NFIzPzl{
CA755 M4m5R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXHzOFJCOTBizszN NVe5THVpPiCm NIjI[3ZKSzVyPUCuNFE1KM7:TR?= NWXxSJN4QTV{NkW3NC=>
CHO MmrLS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoraNVAh|ryP MUi2JIQ> Ml7BTWM2OD1yLkC2JO69VQ>? MVO5OVI3PTdy
Panc02 NVvvN3BZT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDx[FdLOTBizszN M32wSFYh\A>? NEPuPIpKSzVyPUCuNFY2KM7:TR?= MUS5OVI3PTdy
PC14 NGr2d4xEgXSxdH;4bYMhSXO|YYm= NYLNWG02PCCm NIT6TnNFVVOR NGWyS4pKSzVyPUCuNlkh|ryP NWKyN3g5QTh{MkW0Ni=>
HOP62 MVPDfZRwfG:6aXOgRZN{[Xl? MWm3NkBp NVL4UZgyUUN3ME2wMlAxPCEQvF2= NV;nWIZXQTh5M{[2NS=>
SF-295 NWf4OVQ3S3m2b4TvfIlkKEG|c3H5 NIPYZXU4OiCq M4riTGlEPTB;MD6wN|Uh|ryP NV\qVJNkQTh5M{[2NS=>
MDA-MB-361 MWDDfZRwfG:6aXOgRZN{[Xl? Mn\RNUBp Mn\FSG1UVw>? Mom4R5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDLV3CMVM3OSCneIDy[ZN{cW6pIFzhZ3ohf2m2aDDJR|UxKG:oIECuNFEzKM7:TR?= MknJNVA{QTV2OUC=
MDA-MB-361 NH\Z[olEgXSxdH;4bYMhSXO|YYm= Mn3tNUBp NHnx[3dFVVOR NGHIfGVEgXSxdH;4bYNqfHliYXfhbY5{fCCPRFGgUWIhOzZzIHX4dJJme3OrbnegZ4Fz[m:6eYDldJRq\GG|ZTDzeGNRTzJid3n0bEBKSzVyIH;mJFAvODF6IN88US=> MkDxNVA{QTV2OUC=
CCRF-SB NV\6bYdrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MWC0JIQ> Mn3ySG1UVw>? MUjJR|UxRTBwMEGg{txO NGjoS3gyODRzMUS3Oi=>
CHO-K1 NH;oeXpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV20JIQ> MWjEUXNQ M3rMU2lEPTB;MD60JO69VQ>? NHL4cJYyODRzMUS3Oi=>
KB M4mxSWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PXelQh\A>? MUPEUXNQ M3LMTGFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[JJ2\y2{ZYPpd5RidnRiS1K3SEB4cXSqIFnDOVAhd2ZiMD62OUDPxE1? M3TzbFExPDFzNEe2
KB M3L2cGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUCyXXZvPCCm MorvSG1UVw>? NUDLR5NuSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRmNidXC5aYToJGlEPTBib3[gNE4xPiEQvF2= NEfrc4QyODRzMUS3Oi=>
KB MkjtS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV60JIQ> M2C2O2ROW09? MUDBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuETVTSJJdqfGhiSVO1NEBw\iByLkWg{txO M1H4UlExPDFzNEe2
KB M3zrbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGKzSXI1KGR? M1XMU2ROW09? M4LvOmFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[JJ2\y2{ZYPpd5RidnRiS1LWNlBEKHerdHigTWM2OCCxZjCwMlQh|ryP M4P0cFExPDFzNEe2
HTB-54 NXLZdlQ1S3m2b4TvfIlkKEG|c3H5 MlTyNVAxKM7:TR?= NIXlSJBKSzVyPUGuNUDPxE1? MkXoNVA1PzZ6NkG=
MEL-AC NEn5[FhEgXSxdH;4bYMhSXO|YYm= NF;QfW0yODBizszN NEXSW41KSzVyPUGuNkDPxE1? MnrMNVA1PzZ6NkG=
DU145 NYW4TYtIS3m2b4TvfIlkKEG|c3H5 NE\ZbZo4OiCq MkHnTWM2OD1yLkC1Nlgh|ryP NWLFXXZSOTB2N{myPFI>
K562R NHjiVJFEgXSxdH;4bYMhSXO|YYm= MVe3NkBp MlrGTWM2OD1yLkCxOVYh|ryP MXKxNFQ4QTJ6Mh?=
PACA-2 M2K0NGN6fG:2b4jpZ{BCe3OjeR?= MXe2JIQ> Mo\hSWQ2OD1yLkCwOlMh|ryP M2rvXVExPDl6MkG0
3Y1 MnP5R5l1d3SxeHnjJGF{e2G7 NXLRcHVmPzJiaB?= NFH6XXJIUTVyPUCuNFA{QCEQvF2= MWGxNFY6OTdzMB?=
P388 NFPITYlEgXSxdH;4bYMhSXO|YYm= MUG3NkBp MlS2S2k2OD1yLkCxNkDPxE1? M3vER|ExPjlzN{Gw
HMV-1 NFr5cJlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NInrZWFKSzVyPUCuNlgh|ryP NH22bYUyODZ7OESzOi=>
MKN-1 MnXNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH;hUnBKSzVyPUCuOlQh|ryP MnLvNVA3QTh2M{[=
PC14 NUn3Tmd5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnq2TWM2OD1zLkmg{txO M1zlOVExPjl6NEO2
T24 NXPKfpdIT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjv[mZpUUN3ME2wMlY1KM7:TR?= M3vyZVExPjl6NEO2
U937 NUD6OoVYTnWwY4Tpc44hSXO|YYm= MUiyOEBp NHrKXoVFVVOR NVu5e|R[UW6qaXLpeJMhcW6lb4Lwc5JifGmxbjDv[kBcO0ifdHj5cYllcW6nIIfpeIghUUN3MDDv[kAxNjFizszN MX:xNFc5ODlzMR?=
H460pv8/eto NF;C[plEgXSxdH;4bYMhSXO|YYm= M3\EeFExOCEQvF2= NWHyPZhSPDhiaB?= M2ezUHNidGmwZR?= Mon6TWM2OD1yLkCyJO69VQ>? NHLIdIEyODd6MEmxNy=>
H460pv8 NX7JT41qS3m2b4TvfIlkKEG|c3H5 M33WclExOCEQvF2= M4nxNFQ5KGh? MoXHV4FtcW6n NWrjSpZ1UUN3ME2wMlAxPCEQvF2= MVuxNFc5ODlzMx?=
MDA-231 M1\1eGN6fG:2b4jpZ{BCe3OjeR?= NWrjfWJ2OTByIN88US=> M4nqblQ5KGh? M{D6PHNidGmwZR?= NUfUTFFjS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTBMVI{OSClZXzsJEhGWi1uRVfGVkstdXW2YX70JJA2Oylid3n0bEBKSzVyIH;mJFAvODB4IN88US=> NH\wc|AyODd6MEmxNy=>
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M14 MkjZR5l1d3SxeHnjJGF{e2G7 MoDkNVAxKM7:TR?= NFTzd3c1QCCq MmfFS2k2OD1yLkG4JO69VQ>? Mn\QNlM5PzF7MEW=
MALME-3M NV73WYFbS3m2b4TvfIlkKEG|c3H5 MVexNFAh|ryP MoTtOFghcA>? M1L2WWdKPTB;MD6xNkDPxE1? NEnmbFEzOzh5MUmwOS=>
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OVCAR-8 NIrDXWlEgXSxdH;4bYMhSXO|YYm= NETseZMyODBizszN NWf3WVFjPDhiaB?= MYDHTVUxRTBwMTFOwG0> NYrDPVVFOjN6N{G5NFU>
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MCF7 M1HMZmZ2dmO2aX;uJGF{e2G7 Ml\oOUDPxE1? MVKyOEBp MoT2R4VtdHWuYYKgeZB1[Wun NVLlTI17OjN7N{OyNVM>
MCF7 MVHGeY5kfGmxbjDBd5NigQ>? NWHCcVM5PSEQvF2= M17hXVI1KGh? M2n2UmFvfGmxeHnkZY51KGGldHn2bZR6KGG|c3Xzd4VlKGG|IHnueJJi[2WubIXsZZIhdWGub375cIRq[WymZXj5[IUhdGW4ZXy= NGTGc48zOzl5M{KxNy=>
MCF7 MmD4SpVv[3Srb36gRZN{[Xl? NULIXlhuPSEQvF2= M4LUTFI1KGh? MUHD[YxtfWyjcjD1dJRic2V? MXyyN|k4OzJzMx?=
MDA-MB-231 MXfGeY5kfGmxbjDBd5NigQ>? MVy1JO69VQ>? M1m2WFI1KGh? NFu1NYFE\WyudXzhdkB2eHSja3W= MXuyN|k4OzJzMx?=
MDA-MB-231 MlTFSpVv[3Srb36gRZN{[Xl? MXq1JO69VQ>? M1zoWFI1KGh? MUTBcpRqd3irZHHueEBi[3Srdnn0fUBie3Onc4Pl[EBieyCrboTyZYNmdGy3bHHyJI1idG:weXzkbYFt\GWqeXTlJIxmfmWu NXH4eY5TOjN7N{OyNVM>
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MDA-MB-435 MUHDfZRwfG:6aXOgRZN{[Xl? M3rsclExOCEQvF2= NV7WV|ZwPDhiaB?= NUi4SlBJUUN3ME2wMlE2KM7:TR?= MkjUNlQxOjh2NE[=
AS49-RAW NWW2XJF6S3m2b4TvfIlkKEG|c3H5 M4iyOlExOCEQvF2= M{WwV|Q5KGh? MU\JR|UxRTN{Lke4JO69VQ>? NE\YNpUzPDB5N{WyPC=>
Caov3 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH;0SpM4OiCq MnjITWM2OD1yLkGg{txO NXvIUmQyOjRyOUWwPFk>
LNcaP M2r6bGZ2dmO2aX;uJGF{e2G7 NUnaTJo2OyEQvF2= MnjoNlQhcA>? Ml;UTY5kemWjc3Wgc4Yh[2ynYY\l[EBk[XOyYYPlMVkhdGW4ZXy= NEnqVZgzPDB7NUC4PS=>
LNcaP MlviSpVv[3Srb36gRZN{[Xl? MofzN{DPxE1? MY[yOEBp Mm\SWZAuemWpdXzheIlwdiCxZjDwOVMh\XiycnXzd4lwdg>? MVuyOFA6PTB6OR?=
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RPMI-8402 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2DISGlEPTB;NUOuPFYhdk1? M1jncXNCVkeHUh?=
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SCC-9 NFzJXY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NW\aOW13UUN3ME2zMlkyOzB7IN88US=> MnTxV2FPT0WU
CP50-MEL-B NYTiUm9DT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M3jOU2lEPTB;Mz65PFcxOyEQvF2= M4LBdHNCVkeHUh?=
NCI-H345 M1q1Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHi4dpBKSzVyPUSuNFEyPzRizszN NGPFOJpUSU6JRWK=
COR-L88 NGmxO5RIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4DoV2lEPTB;ND6zPVU5KM7:TR?= NVzCVHdnW0GQR1XS
RCM-1 MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVXOXW11UUN3ME20MlQ6Ojd{IN88US=> MW\TRW5ITVJ?
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DMS-53 M3ryWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NWjJWJFnUUN3ME22MlE4OTB4IN88US=> MWLTRW5ITVJ?
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BB49-HNC NV;ZVop2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmTBTWM2OD15LkSxO|kyKM7:TR?= M2rUU3NCVkeHUh?=
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... Click to View More Cell Line Experimental Data
In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (172.41 mM)
Water 20 mg/mL (34.48 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl
CAS No. 25316-40-9
Storage powder
in solvent
Synonyms NSC 123127

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

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Molecular Weight Calculator

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03571321 Not yet recruiting Acute Lymphoblastic Leukemia|ALL Childhood|ALL University of Chicago|Incyte Corporation September 5 2019 Phase 1
NCT03571321 Not yet recruiting Acute Lymphoblastic Leukemia|ALL Childhood|ALL University of Chicago|Incyte Corporation September 5 2019 Phase 1
NCT03108300 Not yet recruiting Malignant Soft Tissue Sarcoma Ain Shams University August 30 2019 Phase 2
NCT03108300 Not yet recruiting Malignant Soft Tissue Sarcoma Ain Shams University August 30 2019 Phase 2
NCT03437070 Withdrawn Leiomyosarcoma University of Miami|Janssen Scientific Affairs LLC June 2019 Phase 1
NCT03349281 Recruiting Refractory Acute Lymphoblastic Leukemia|Relapsed Acute Lymphoblastic Leukemia Julio Barredo|Takeda|University of Miami June 2019 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    Why the IC50 of the compound varied over time in our measurement?

  • Answer:

    Sometimes the IC50 value might vary between different batch of compounds due to the slight differences in purity. If different IC50 was seen in the same batch of compound, the problem may lies in the process of dissolvement. Make sure that you see no particles and the solution is clear with your stock solution.

selleck catalog

Topoisomerase Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID