Doxorubicin (Adriamycin) HCl

For research use only.

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127, DOX

684 publications

Doxorubicin (Adriamycin) HCl Chemical Structure

CAS No. 25316-40-9

Doxorubicin (Adriamycin, NSC 123127, DOX) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage, mitophagy and apoptosis in tumor cells. Doxorubicin reduces basal phosphorylation of AMPK. Doxorubicin is used in the concomitant treatment of HIV-infected patients but is found to be at high risk of HBV reactivation.

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Selleck's Doxorubicin (Adriamycin) HCl has been cited by 684 publications

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Biological Activity

Description Doxorubicin (Adriamycin, NSC 123127, DOX) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage, mitophagy and apoptosis in tumor cells. Doxorubicin reduces basal phosphorylation of AMPK. Doxorubicin is used in the concomitant treatment of HIV-infected patients but is found to be at high risk of HBV reactivation.
Targets
AMPK [6]
()
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
L1210 MXXGeY5kfGmxbjDBd5NigQ>? Mk\WTY5pcWKrdHnvckBw\iCUTlGgd5lvfGinc3nzJJdqfGhiRVS1NEBw\iByLkW4JO69VQ>? MX20PVA2OzZ?
Yoshida sarcoma MWPGeY5kfGmxbjDBd5NigQ>? NGjaNWxKdmirYnn0bY9vKG:oIGuzTH1V\FJiaX7jc5Jxd3KjdHnvckB4cXSqIFnDOVAhd2ZiMD6wNkDPxE1? MXuxN|IzQTh4
CHO NYruVHlJT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2HIdGlEPTB;MD6wOkDPxE1? Mo\qNVM2PDd3MB?=
8226 NWq3TXV1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoDTTWM2OD1yLkCzN{DPxE1? MVSxOFQ4PzNy
8226/ADR MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWHLUGxzUUN3ME2wMlU3KM7:TR?= M3HtXlE1PDd5M{C=
MCF7/ADR MnvsS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHJR|UxRTNwMTFOwG0> MV[xOFQ4PzNy
H69 NYfObHB2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1LncGlEPTB;MD64O{DPxE1? NWTEelRIOTR5OUW4OS=>
N417 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn\PTWM2OD1yLkKzJO69VQ>? M33xTlE1Pzl3OEW=
H9 NHvKNYlIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIez[4dKdmirYnn0bY9vKG:oIFi5JINmdGy|IHPodo9vcWOjbHz5JIlv\mWldHXkJJdqfGhidHjlJGhKXi1zIDjIPU9KUUmEKTD3bZRpKEmGNUCgc4YhOC5yMTFOwG0> NHOwVYEyPTR6Nkix
T-C8166 MUnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2r0bWlFPTB;MD6wNUDPxE1? MW[xOVQ5Pjhz
T-H9 NEHucWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEP6XY9KTDVyPUCuNFEh|ryP MX:xOVQ5Pjhz
T-MT4 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NY\FU|BrUUR3ME2wMlAyKM7:TR?= NUX4OHZKOTV2OE[4NS=>
SKMEL-5 NXSyUXE4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\NSWQ2OD1|LkKg{txO MnLDNVYyOzd3Mx?=
MLM NF7JXlVEgXSxdH;4bYMhSXO|YYm= M3qzbGVFPTB;MD6xN{DPxE1? MnPaNVg4PTN3MB?=
KBM-3 MoX4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGXGUppKSzVyPUCuNFI3KM7:TR?= NFnu[nMyQTl3OEe3
HT-29 Mm\nS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV;NfZh{OC5yMTFOwIc> MkfOOFghcA>? Ml;kRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdIm= MonBNlc4QDR2OR?=
EMT6 MkL1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MofoNE42KM7:TR?= NIDhW2oyKGh? MV;Jcohq[mm2aX;uJI9nKHC{b3zp[oVz[XSrb36gZpkhdWWjc4XybY5oKG[xcjDzeZJ3cX[rbneg[pJi[3Srb37zJJdqfGhiRVS1NEBw\iByLkCwNFEh|ryP NWPPXY9{PzR3Mk[3OC=>
H2981 MkfhR5l1d3SxeHnjJGF{e2G7 MUDJR|UxRTBwNTFOwG0> NEHkN4g4PzNzMEKz
LOX MkGzR5l1d3SxeHnjJGF{e2G7 MVLJR|UxRTBwMESyJO69VQ>? M{S0TFc5PTN|M{G=
C38 NH22WW5EgXSxdH;4bYMhSXO|YYm= MX3JR|UxRTBwMEGg{txO M1nlb|gxPTd{OUG=
EAC MV7DfZRwfG:6aXOgRZN{[Xl? MnrqO|IhcA>? M{HZU2lEPTB;MD6zOUDPxE1? NI\xbnI5OzVyMEi3
SKVLB M13T[2N6fG:2b4jpZ{BCe3OjeR?= MYTJR|UxRTVwNjFOwG0> M2LuR|g2PzZ7MUS=
AUC375 NFvXWYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlzlTWM2OD1yLkGxNkDPxE1? MXO4OlQ5PjBy
A2780-C25 MnTlR5l1d3SxeHnjJGF{e2G7 MlrIR5l1d3SxeHnjbZR6KGGpYXnud5Qhd3ijbHnwcIF1cW5vcnXzbZN1[W62IFGyO|gxNUN{NTD3bZRpKEmFNUCgc4YhOC5yNkOg{txO NEXqT2E5QDNzN{W1
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MXF7 M2TReGN6fG:2b4jpZ{BCe3OjeR?= NXHpS5J4SW62aYT1cY9zKGGldHn2bZR6KGGpYXnud5Qhe2Wwc3n0bZZmKE2[Rkege4l1cCCLQ{WwJI9nKDBwMEK4JO69VQ>? NILpbYg5QTZyNUW4
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MXF7 MVHDfZRwfG:6aXOgRZN{[Xl? MnizRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4Sg[I95d3K3YnnjbY4uemW|aYP0ZY51KE2[Rkege4l1cCCLQ{WwJI9nKDFwMUeyJO69VQ>? NGjQRnE5QTZyNUW4
WiDr MnXtR5l1d3SxeHnjJGF{e2G7 MmTnRYN1cX[rdImgZYdicW6|dDDz[Y5{cXSrdnWgW4lFeiClZXzsJJdqfGhiSVO1NEBw\iByLkC1N{DPxE1? Ml;3PFk3ODV3OB?=
MCF M2DoNmZ2dmO2aX;uJGF{e2G7 MV\S[ZZmenOjbDDv[kBRNWeyLX3l[IlifGWmIH31cJRq\HK3ZzDy[ZNqe3SjbnPlJIF{e2W|c3XkJIF{KGWwaHHuZ4Vu\W62IHnuJIN6fG:2b4jpZ4l1gSCxZjDh[JJq[W27Y3nu NF\HSlU5QTh2MUWx
C6 NGLLPGNEgXSxdH;4bYMhSXO|YYm= NFLOUW0zNjVibXevcWw> MWS3NkBp MXnEUXNQ MXLDfZRwfG:6aXPpeJkh[WejaX7zeEBz[XRiQ{[gZ4VtdHNiYYSgOVAhfWdxbVygeI8hOi53IH3nM41N NFO5SYM5QTh6NkC0
C6 NXvHV2NpTnWwY4Tpc44hSXO|YYm= MmfCNk42KG2pL33M MYS0JIg> Mlf1SG1UVw>? Ml\mTY5pcWKrdHnvckBw\iC2dXL1cIlvKHCxbInt[ZJqgmG2aX;u MWi4PVg5PjB2
C6 M{jk[GN6fG:2b4jpZ{BCe3OjeR?= NFHYV|gzNjVibXevcWw> NVnLboVLPSCq M1LSV2ROW09? MmjSR5l1d3SxeHnjbZR6KGG|c3Xzd4VlKGG|IHPlcIwhemWuZXHz[S=> NFvkVG85QTh6NkC0
SKVLB M2f1SmN6fG:2b4jpZ{BCe3OjeR?= M1r6RVczKGh? MlnOTWM2OD14LkW0JO69VQ>? MXq5NFA{PTJy
MCF7/VP16 NVHVfGQ5S3m2b4TvfIlkKEG|c3H5 NEfVTlg2OCEQvHevcWw> M3T2RlczKGh? MV7JR|UxRTF2LkKg{txO NF[1[GY6ODJ{N{my
CA755 M3LZRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEDiO44yOCEQvF2= NWfyPI1kPiCm MX7JR|UxRTBwMEG0JO69VQ>? NHK4cHU6PTJ4NUew
CHO Ml7aS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWSxNEDPxE1? M2DtfFYh\A>? MnzVTWM2OD1yLkC2JO69VQ>? M4\pclk2OjZ3N{C=
Panc02 NF\BXGZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGrzTokyOCEQvF2= NEDMUII3KGR? NHPne5BKSzVyPUCuNFY2KM7:TR?= MnPYPVUzPjV5MB?=
PC14 Mlr0R5l1d3SxeHnjJGF{e2G7 MXq0JIQ> MmDBSG1UVw>? M1PrfmlEPTB;MD6yPUDPxE1? M17wN|k5OjJ3NEK=
HOP62 Mk\iR5l1d3SxeHnjJGF{e2G7 NFr0Vos4OiCq MkCxTWM2OD1yLkCwOEDPxE1? M{\NcVk5PzN4NkG=
SF-295 NV;tcYJ3S3m2b4TvfIlkKEG|c3H5 NUPuTm11PzJiaB?= NWfBOWFuUUN3ME2wMlA{PSEQvF2= MXW5PFc{PjZz
MDA-MB-361 NFfyS|ZEgXSxdH;4bYMhSXO|YYm= MUCxJIg> NXP1UWx3TE2VTx?= NVjJRoxZS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTBMW1DNTN4MTDlfJBz\XO|aX7nJGxi[1pid3n0bEBKSzVyIH;mJFAvODF{IN88US=> M2LJZlExOzl3NEmw
MDA-MB-361 Ml;WR5l1d3SxeHnjJGF{e2G7 M3Pk[VEhcA>? M{[yU2ROW09? NUSwcJFZS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTBJG1DKDN4MTDlfJBz\XO|aX7nJINiemKxeInw[ZB1cWSjc3Wgd5REWEd{IIfpeIghUUN3MDDv[kAxNjBzODFOwG0> MX2xNFM6PTR7MB?=
CCRF-SB MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH75cYs1KGR? NE\4R25FVVOR MkX4TWM2OD1yLkCxJO69VQ>? MXexNFQyOTR5Nh?=
CHO-K1 NEPwfGRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV20JIQ> MoDISG1UVw>? M3TUWGlEPTB;MD60JO69VQ>? M2rGOVExPDFzNEe2
KB Mme0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXq0JIQ> NUD2SGpQTE2VTx?= NXmxfWg2SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRldFKHerdHigTWM2OCCxZjCwMlY2KM7:TR?= MVWxNFQyOTR5Nh?=
KB MkDuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEDnfoI1KGR? MYfEUXNQ NYnNSY5mSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRmNidXC5aYToJGlEPTBib3[gNE4xPiEQvF2= NGHGUoIyODRzMUS3Oi=>
KB NH\2SGhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkK3OEBl NInpUlJFVVOR MonuRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDkdpVoNXKnc3nzeIFvfCCNQl3EVkB4cXSqIFnDOVAhd2ZiMD61JO69VQ>? MUixNFQyOTR5Nh?=
KB Mo\LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MonmOEBl NELpenNFVVOR MlvKRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDkdpVoNXKnc3nzeIFvfCCNQm[yNGMhf2m2aDDJR|UxKG:oIECuOEDPxE1? NFjEOJEyODRzMUS3Oi=>
HTB-54 NUTiSpZkS3m2b4TvfIlkKEG|c3H5 MVexNFAh|ryP NHntVFBKSzVyPUGuNUDPxE1? MVSxNFQ4Pjh4MR?=
MEL-AC MX3DfZRwfG:6aXOgRZN{[Xl? M4\IUFExOCEQvF2= NHrRdHJKSzVyPUGuNkDPxE1? NXnyWpBnOTB2N{[4OlE>
DU145 MmfTR5l1d3SxeHnjJGF{e2G7 M3vrb|czKGh? Mmf0TWM2OD1yLkC1Nlgh|ryP M{PWZVExPDd7Mkiy
K562R M2TETmN6fG:2b4jpZ{BCe3OjeR?= MnfvO|IhcA>? MX\JR|UxRTBwMEG1OkDPxE1? MlfQNVA1Pzl{OEK=
PACA-2 MkiwR5l1d3SxeHnjJGF{e2G7 NYfZcm9{PiCm M4r1PWVFPTB;MD6wNFY{KM7:TR?= MkjJNVA1QTh{MUS=
3Y1 NVraNGd1S3m2b4TvfIlkKEG|c3H5 M2\1blczKGh? MUjHTVUxRTBwMECzPEDPxE1? M37FZ|ExPjlzN{Gw
P388 NUXreIl1S3m2b4TvfIlkKEG|c3H5 MX63NkBp M3\GWGdKPTB;MD6wNVIh|ryP MY[xNFY6OTdzMB?=
HMV-1 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWDDXoJZUUN3ME2wMlI5KM7:TR?= NHLSUYkyODZ7OESzOi=>
MKN-1 M3PXbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV;1OW15UUN3ME2wMlY1KM7:TR?= NXzt[m9nOTB4OUi0N|Y>
PC14 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkXJTWM2OD1zLkmg{txO M1:wZVExPjl6NEO2
T24 MV3Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTBwNkSg{txO M2nnXlExPjl6NEO2
U937 Ml60SpVv[3Srb36gRZN{[Xl? M1HsbFI1KGh? MVvEUXNQ M13vT2lvcGmkaYTzJIlv[2:{cH;yZZRqd25ib3[gX|NJZXSqeX3p[Ilv\SC5aYToJGlEPTBib3[gNE4yKM7:TR?= MX[xNFc5ODlzMR?=
H460pv8/eto MVLDfZRwfG:6aXOgRZN{[Xl? Ml\DNVAxKM7:TR?= M3\qN|Q5KGh? NYjqcVlLW2GuaX7l M1vyW2lEPTB;MD6wNkDPxE1? M{PpV|ExPzhyOUGz
H460pv8 NFXmZldEgXSxdH;4bYMhSXO|YYm= MmPGNVAxKM7:TR?= MWe0PEBp NV\YcIxXW2GuaX7l Ml7MTWM2OD1yLkCwOEDPxE1? MXqxNFc5ODlzMx?=
MDA-231 NY\DV3RYS3m2b4TvfIlkKEG|c3H5 M4X3TVExOCEQvF2= NWrzb3dTPDhiaB?= MX\TZYxqdmV? NIfQR2REgXSxdH;4bYNqfHliYXfhbY5{fCCPRFGtNlMyKGOnbHygLGVTNSyHR1\SL{xufXSjboSgdFU{MSC5aYToJGlEPTBib3[gNE4xODZizszN NVjxXppKOTB5OEC5NVM>
MDA-468 MlnuR5l1d3SxeHnjJGF{e2G7 NIHxfocyODBizszN NYPleYFCPDhiaB?= M4PaZ3NidGmwZR?= MnXQR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDLUS2PEBk\WyuIDjFVk0tKGGvcHzp[olm\CCHR1\SMEBufXSjboSgdFU{MSC5aYToJGlEPTBib3[gNE4xODVizszN M2DSelExPzhyOUGz
NCI-H226 NVrBVZI2S3m2b4TvfIlkKEG|c3H5 MUSxNFAh|ryP MoHWOFghcA>? M3vU[HNidGmwZR?= NXz2eo5NS3m2b4TvfIlkcXS7IHHnZYlve3RiTlPJMWgzOjZiY3XscEApdXW2YX70JJA2Oylid3n0bEBKSzVyIH;mJFAvODB6IN88US=> NYLUVItTOTB5OEC5NVM>
NCI-H322 MWXDfZRwfG:6aXOgRZN{[Xl? NYTxO2pEOTByIN88US=> M1T5flQ5KGh? M2SxfXNidGmwZR?= MVvDfZRwfG:6aXPpeJkh[WejaX7zeEBPS0lvSEOyNkBk\WyuIDjteZRidnRicEWzLUB4cXSqIFnDOVAhd2ZiMD6wN{DPxE1? NHPwSZUyODd6MEmxNy=>
NCI-H358 NIPsfI1EgXSxdH;4bYMhSXO|YYm= M2H0PFExOCEQvF2= NIW3d4k1QCCq M2qwW3NidGmwZR?= NUnZV2t2S3m2b4TvfIlkcXS7IHHnZYlve3RiTlPJMWg{PThiY3XscEApdXW2YX70JJA2Oylid3n0bEBKSzVyIH;mJFAvOTNizszN M{D3cFExPzhyOUGz
NCI-H647 NEf3PIdEgXSxdH;4bYMhSXO|YYm= NWHEbFYyOTByIN88US=> MXW0PEBp NX7JWWZHW2GuaX7l NHLhNoFEgXSxdH;4bYNqfHliYXfhbY5{fCCQQ1mtTFY1PyClZXzsJEhufXSjboSgdFU{MSC5aYToJGlEPTBib3[gNE4xODFizszN MUGxNFc5ODlzMx?=
SKBR-3 M2HtS2N6fG:2b4jpZ{BCe3OjeR?= NEPDUJoyODBizszN MUC0PEBp MYTTZYxqdmV? NXnuSXRGS3m2b4TvfIlkcXS7IHHnZYlve3RiU1vCVk0{KGOnbHygLGVTNWGvcHzp[olm\CCnclKyMI12fGGwdDDwOVMqKHerdHigTWM2OCCxZjCwMlAzKM7:TR?= MUmxNFc5ODlzMx?=
T47D MXXDfZRwfG:6aXOgRZN{[Xl? NIfRUFEyODBizszN M2G1TVQ5KGh? Mn3jV4FtcW6n NVHT[mhES3m2b4TvfIlkcXS7IHHnZYlve3RiVES3SEBk\WyuIDjFVkstdXW2YX70JJA2Oylid3n0bEBKSzVyIH;mJFAvODB5IN88US=> MWGxNFc5ODlzMx?=
ZR-75-1 M1;qWWN6fG:2b4jpZ{BCe3OjeR?= NUjGWmQ4OTByIN88US=> NXW4TlVLPDhiaB?= MoO0V4FtcW6n M{X0SmN6fG:2b4jpZ4l1gSCjZ3HpcpN1KFqULUe1MVEh[2WubDCoSXIsNHCpcjuscZV1[W62IIC1N{khf2m2aDDJR|UxKG:oIECuNFA6KM7:TR?= MV[xNFc5ODlzMx?=
L1210 MX7DfZRwfG:6aXOgRZN{[Xl? M{LvcWlEPTB;MD6wNlUh|ryP NFf6W4cyODh7MEG2Oy=>
D40 NFfXOJpEgXSxdH;4bYMhSXO|YYm= MkKzNlQhcA>? NWPHU4MzTE2VTx?= NE\2VpVKSzVyPUGuNVczKM7:TR?= NHjXdmsyODl3NkKxOC=>
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A2780 NEXVXoZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWfz[Ig3UUN3ME22PE44QSCwTR?= M2fNV3NCVkeHUh?=
Daudi M{jCPWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Mn7lTWM2OD14OD65OkBvVQ>? MnT5V2FPT0WU
GI-1 MnLTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlHPTWM2OD14OT62OUBvVQ>? MoDQV2FPT0WU
SW962 MXvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX;JR|UxRTZ7Lkm1JI5O NFyy[3JUSU6JRWK=
MFE-296 M1ezdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1nJWmlEPTB;N{CuOFkhdk1? MoXTV2FPT0WU
H4 Mn:zS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV7wfY1PUUN3ME23NU42PiCwTR?= MknNV2FPT0WU
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HuCCT1 NVjxOIxWT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NFm5OJlKSzVyPUeyMlk{KG6P MnPLV2FPT0WU
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DU-145 MlvuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mlm0TWM2OD15Mz63N{BvVQ>? MoHTV2FPT0WU
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MDA-MB-468 Mo\lS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYTJR|UxRTd4LkG4JI5O M33qNnNCVkeHUh?=
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TE-1 M{Thcmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTd4LkO1JI5O M32x[nNCVkeHUh?=
CMK MlnOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUXJR|UxRTd5LkSyJI5O NUG5WXhsW0GQR1XS
LB996-RCC M{nUZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVy3cpFtUUN3ME23PE41PSCwTR?= NWfkU4xsW0GQR1XS
NCI-H1650 NYf6Rm14T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV\QcnN2UUN3ME23PE43OiCwTR?= NF;YTJdUSU6JRWK=
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TE-12 MWDHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NIHV[4lKSzVyPUixMlA3KG6P M4PpRnNCVkeHUh?=
MKN45 NGTU[Y9Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRThzLkWxJI5O M4POeHNCVkeHUh?=
LoVo Mn31S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2fodWlEPTB;OEGuPFIhdk1? NGCzN29USU6JRWK=
SW1573 MnTpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXyzOG5xUUN3ME24Nk45OSCwTR?= MV7TRW5ITVJ?
ETK-1 M1G3fWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1TvcGlEPTB;OEOuNFEhdk1? Mnz6V2FPT0WU
ABC-1 NHz2[IpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXXJR|UxRTh|LkS1JI5O NG\NV4RUSU6JRWK=
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BC-1 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTh4Lke2JI5O NFS1R45USU6JRWK=
PANC-10-05 NHXL[HpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3Lj[2lEPTB;OEiuOFUhdk1? NFXoWnJUSU6JRWK=
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TE-441-T M4XDNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFPTSZJKSzVyPUi5MlgzKG6P NGnrZ4hUSU6JRWK=
KM-H2 NInR[|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2jFPGlEPTB;OUCuOlghdk1? NGrEPJZUSU6JRWK=
SH-4 MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWK4UnI3UUN3ME25Nk4yOSCwTR?= M2K4R3NCVkeHUh?=
SF268 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUmxRmdZUUN3ME25Nk41OiCwTR?= NITnOWpUSU6JRWK=
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GI-ME-N MmjvS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV;JR|UxRTl|LkOzJI5O NVu2RnlbW0GQR1XS
JEG-3 NIfPXFZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPxTWM2OD17NT6xNkBvVQ>? M1HoWXNCVkeHUh?=
J-RT3-T3-5 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkTxTWM2OD17Nj6zNUBvVQ>? Moj2V2FPT0WU
K5 MlTzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWKxWlREUUN3ME25Ok46KG6P NFvN[2xUSU6JRWK=
EFO-27 MnzqS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYH5bVk{UUN3ME25O{41PCCwTR?= MVfTRW5ITVJ?
SW1783 NX7KRXY2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYD2RpFQUUN3ME25O{46QSCwTR?= NV3XSnlyW0GQR1XS
CAL-39 NWLy[WdsT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVHURYlsUUN3ME25PE4zPCCwTR?= NVzSOodPW0GQR1XS
LS-441N NUXVVZRCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2HkV2lEPTB;OUmuNFYhdk1? MXPTRW5ITVJ?
G-402 M{nWNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIeyb4RKSzVyPUm5MlA6KG6P MmfzV2FPT0WU
MES-SA NYLxOXZiT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NETy[3RKSzVyPUm5MlU{KG6P NYC1XJRyW0GQR1XS
FADU M4WxdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1PER2lEPTB;MUCyMlE5KG6P MVnTRW5ITVJ?
DJM-1 MoOzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXrzN5pzUUN3ME2xNFIvOjVibl2= NWjUNG8{W0GQR1XS
SU-DHL-1 NVP6SWViT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2Lz[WlEPTB;MUCyMlUhdk1? NUPEb5d7W0GQR1XS
KYSE-410 NHXY[opIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYPJR|UxRTFyMz60OkBvVQ>? NX2xR2JbW0GQR1XS
NCI-H1618 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVXJR|UxRTFyND6zOUBvVQ>? NUXpO3U1W0GQR1XS
AU565 NVvZNVFbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1jkcGlEPTB;MUC0MlUyKG6P Mne5V2FPT0WU
SF126 MnrRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmT5TWM2OD1zMESuOVghdk1? NIDKVI1USU6JRWK=
MMAC-SF NXTyU3M1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVjJR|UxRTFyNj60PEBvVQ>? M2\l[3NCVkeHUh?=
ChaGo-K-1 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoTSTWM2OD1zMEeuPVUhdk1? MW\TRW5ITVJ?
NCI-H596 NEXmeYZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI[5T5NKSzVyPUGwPE4xOSCwTR?= NFXjb4NUSU6JRWK=
LXF-289 NUjvZVdqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NY[xfIpbUUN3ME2xNFkvOjRibl2= MX\TRW5ITVJ?
HCC1569 M{fKS2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3\OZmlEPTB;MUC5MlM1KG6P M4\lZnNCVkeHUh?=
NCI-H2170 MknhS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHHVXpZKSzVyPUGwPU46OiCwTR?= NFe2T49USU6JRWK=
ARH-77 MmXFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkLITWM2OD1zMEmuPVkhdk1? Mk\MV2FPT0WU
BOKU NETBNpJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3vyfWlEPTB;MUGwMlg4KG6P M2izVXNCVkeHUh?=
SJSA-1 MWrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF3MWFNKSzVyPUGxNU4xPSCwTR?= NY\hPGVsW0GQR1XS
SW626 Mk\RS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEPTT4xKSzVyPUGxNU44OSCwTR?= MVzTRW5ITVJ?
EHEB MkPpS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkX1TWM2OD1zMUKuOVkhdk1? M{T2VXNCVkeHUh?=
TE-10 MXXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mo\iTWM2OD1zMUKuOlghdk1? MknRV2FPT0WU
MZ7-mel NFfxWItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFHxfWZKSzVyPUGxNk45PCCwTR?= MkHlV2FPT0WU
SAS MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGTESnRKSzVyPUGxOE41PSCwTR?= NEX5OmpUSU6JRWK=
TK10 M1LM[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVTmSHVxUUN3ME2xNVQvPiCwTR?= NWXzdlhEW0GQR1XS
SW13 Mn3uS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIX0co1KSzVyPUGxOU4zPiCwTR?= NX\hUmhmW0GQR1XS
HCC1937 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFHET|NKSzVyPUGxOU45PCCwTR?= NX3mXosxW0GQR1XS
SK-NEP-1 Ml[1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mo\ETWM2OD1zMU[uNlYhdk1? MW\TRW5ITVJ?
OVCAR-5 Mn31S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYLJR|UxRTFzNj64NUBvVQ>? MlWxV2FPT0WU
SW837 MnG3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4f6dGlEPTB;MUG3Mlk2KG6P M{TNS3NCVkeHUh?=
BFTC-909 NVPDTo12T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mo\XTWM2OD1zMUiuOFghdk1? MX3TRW5ITVJ?
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SF539 M4fuNGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWjJR|UxRTF{MT63NkBvVQ>? NEPlZ5VUSU6JRWK=
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LB647-SCLC M3e3XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnLtTWM2OD1zMkOuN|Uhdk1? MXfTRW5ITVJ?
NCI-H1703 NWTGUpRRT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVfJR|UxRTF{Nj60NkBvVQ>? M2j4Z3NCVkeHUh?=
KURAMOCHI NYrxNppmT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVm5XldEUUN3ME2xNlYvPzZibl2= M1vYdHNCVkeHUh?=
ATN-1 MlXwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGPJ[45KSzVyPUGyO{44KG6P NV;KXoFqW0GQR1XS
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RPMI-7951 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4nWeWlEPTB;MUOwMlE1KG6P NIXx[o1USU6JRWK=
NCI-H1648 NFvM[pFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHjUZZpKSzVyPUGzNU46PCCwTR?= MlLQV2FPT0WU
NCI-H64 MmSzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3XzcmlEPTB;MUOyMlY2KG6P MnjQV2FPT0WU
HT-3 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFezelBKSzVyPUGzN{4xPCCwTR?= NYPpfllOW0GQR1XS
NCI-H1770 NHjyNIJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M17sWWlEPTB;MUOzMlIyKG6P MVjTRW5ITVJ?
NB13 NXflVnZpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVPJR|UxRTF|ND61OUBvVQ>? M2C5Z3NCVkeHUh?=
NCI-H747 NFTocVJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnLzTWM2OD1zM{WuNFIhdk1? MYnTRW5ITVJ?
ML-2 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkHSTWM2OD1zM{WuNVYhdk1? NEjlVoFUSU6JRWK=
CA46 MlzOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\rTGlEPTB;MUO2Mlg5KG6P NY\XZZY3W0GQR1XS
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JiyoyeP-2003 M{nzXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWDJR|UxRTF|Nz64JI5O NF;JTlFUSU6JRWK=
NMC-G1 MmH6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnu0TWM2OD1zM{muNVMhdk1? NITXPHFUSU6JRWK=
U-698-M NYT6cpd3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NEK1OnFKSzVyPUGzPU42KG6P MX\TRW5ITVJ?
LU-99A MlG0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mnu1TWM2OD1zNE[uOVUhdk1? NHv5VZNUSU6JRWK=
NCI-H748 NHy3e3hIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MomxTWM2OD1zNEeuN|Ehdk1? M3jaTnNCVkeHUh?=
SK-MEL-3 MlvNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEP2eldKSzVyPUG0O{43OSCwTR?= MkPyV2FPT0WU
SNB75 M1\pOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkjwTWM2OD1zNEiuOlUhdk1? M2DOcHNCVkeHUh?=
BB65-RCC MoH5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWnmdoFyUUN3ME2xOFkvOzFibl2= MnvvV2FPT0WU
NCI-H1355 M3fZTWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NV7aemZiUUN3ME2xOVIvOjRibl2= NFT5R3dUSU6JRWK=
SCC-25 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUPJR|UxRTF3Mz63N{BvVQ>? MXHTRW5ITVJ?
OAW-42 NYjBdXA{T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYjVXHdvUUN3ME2xOVMvQDRibl2= M4roOnNCVkeHUh?=
MONO-MAC-6 NILmc2ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGjmboJKSzVyPUG1OE4{PiCwTR?= MlW0V2FPT0WU
WSU-NHL MmK5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV7JR|UxRTF3ND61PEBvVQ>? NYj6WpltW0GQR1XS
SNU-423 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXLJR|UxRTF3NT6zOEBvVQ>? NF\hPJlUSU6JRWK=
SBC-1 NXq3Z5pCT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NU\ZeYFOUUN3ME2xOVYvPCCwTR?= NVLlUXY4W0GQR1XS
OVCAR-4 MkL4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4SzbWlEPTB;MUW2Mlc5KG6P MYfTRW5ITVJ?
NCI-H2347 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH7aVpBKSzVyPUG1PE4{PCCwTR?= M37iPHNCVkeHUh?=
CAMA-1 NGfmUphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYn2[FliUUN3ME2xOVkvOzNibl2= MkHTV2FPT0WU
GDM-1 NFLiN4ZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3rDbGlEPTB;MUW5Mlchdk1? MUfTRW5ITVJ?
KNS-81-FD MljoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYf4XGxKUUN3ME2xOlAvOzJibl2= NVXmO|ViW0GQR1XS
no-10 M{G0eWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXf1W|lLUUN3ME2xOlAvOzhibl2= MnLEV2FPT0WU
NCI-H2052 MmjKS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXLZcGlmUUN3ME2xOlAvPDdibl2= NXTrSoFLW0GQR1XS
DB MlTBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVXJR|UxRTF4MT63NUBvVQ>? NH;yNVlUSU6JRWK=
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HCT-15 MVjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX3JR|UxRTJwM{W0OlYh|ryP MV7TRW5ITVJ?
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SNU-475 NWrCdXNET3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M4D5cGlEPTB;Mj65NlU4OiEQvF2= MXjTRW5ITVJ?
COLO-792 M1;XdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXjJR|UxRTNwMEK4O{DPxE1? NIXobYJUSU6JRWK=
YAPC M4jUNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\TWWtKSzVyPUOuN|I5OzJizszN MoDxV2FPT0WU
UACC-812 NFLy[WJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWDJR|UxRTNwM{O3N|gh|ryP M2fwVnNCVkeHUh?=
Mewo M2D3Vmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkThTWM2OD1|LkS2NFg3KM7:TR?= NUWxc4V1W0GQR1XS
COLO-824 M3\3R2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1K5TWlEPTB;Mz61O|M4QCEQvF2= MUHTRW5ITVJ?
SCC-9 MoLFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWTJR|UxRTNwOUGzNFkh|ryP MnOyV2FPT0WU
CP50-MEL-B NYD3emU3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVOyXYVuUUN3ME2zMlk5PzB|IN88US=> MmntV2FPT0WU
NCI-H345 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3viVGlEPTB;ND6wNVE4PCEQvF2= MonLV2FPT0WU
COR-L88 NUXsc4U1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2\3V2lEPTB;ND6zPVU5KM7:TR?= M4DKVHNCVkeHUh?=
RCM-1 M1\UbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoP4TWM2OD12LkS5NlczKM7:TR?= NHPu[45USU6JRWK=
JVM-3 NGr6fmtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M{f5e2lEPTB;ND62NFYyQCEQvF2= M4OzXnNCVkeHUh?=
ECC4 NEHsUlBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mn7ITWM2OD12Lke2OVQyKM7:TR?= NF7wdXBUSU6JRWK=
MDA-MB-453 MYnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVzJR|UxRTRwOEWxN|Qh|ryP NULFcVYzW0GQR1XS
SK-N-FI MlXmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2nsSWlEPTB;NT6yNlg4PyEQvF2= M4nqfHNCVkeHUh?=
D-502MG MnvJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHH5dFJKSzVyPUWuNlQzPjJizszN MlPwV2FPT0WU
NCI-H2227 NF7XZ4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGLDPIhKSzVyPUWuN|YzOzFizszN MX7TRW5ITVJ?
DMS-53 NVOwOGZ3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2rkV2lEPTB;Nj6xO|ExPiEQvF2= MoTGV2FPT0WU
LB771-HNC MlfZS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXG0TIVxUUN3ME22MlQ3QDB4IN88US=> NWD3N3l4W0GQR1XS
BB49-HNC MmrQS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEnQSFRKSzVyPUeuOFE4QTFizszN NEDreJZUSU6JRWK=
MDA-MB-415 M1Hhemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWTJR|UxRTdwNUezNFMh|ryP MmG1V2FPT0WU
TALL-1 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkD1TWM2OD15Lkm2PFgh|ryP MUPTRW5ITVJ?
HCC1395 M{DqVGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYTJR|UxRThwMUWxNlIh|ryP NIjV[ZFUSU6JRWK=
EFE-184 Ml24S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkXCTWM2OD16LkO3NlE3KM7:TR?= M13xXHNCVkeHUh?=
EC-GI-10 M{DoWWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIi2dmpKSzVyPUiuOlg3QDZizszN MYnTRW5ITVJ?
TT NX60WJVpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MUjJR|UxRTlwME[5OlYh|ryP NGewdnlUSU6JRWK=
U-2-OS MmDwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVjJR|UxRTlwOEGzPVMh|ryP M1HLR3NCVkeHUh?=
SHP-77 MnHRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHXYW2xKSzVyPUmuPVAzQSEQvF2= NI[wTppUSU6JRWK=
HCC1143 NH7qSYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NFTvT|hKSzVyPUGwMlQ4QTdizszN M{\OUXNCVkeHUh?=
A498 NXnpVIhkT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmDZTWM2OD1zOD6yPVc3KM7:TR?= MlLlV2FPT0WU
LS-513 Mlv0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWHJR|UxRTF6Lk[wPVMh|ryP NFewPW9USU6JRWK=
K052 NWHtRVFVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MXvJR|UxRTJzLkS0NVgh|ryP NVjkZ3d4W0GQR1XS
SiHa MYHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M4DPVmlEPTB;MkOuN|M4OSEQvF2= M13VRnNCVkeHUh?=
RCC10RGB MkH4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn3VTWM2OD1|MD6wOlEyKM7:TR?= NGLKSYZUSU6JRWK=
NCI-SNU-16 NInJWFRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV;adoJwUUN3ME2zNU4yQDN4IN88US=> MXXTRW5ITVJ?
SNU-C1 MkjrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkPGTWM2OD1|Mj60NVc2KM7:TR?= Mn70V2FPT0WU
C32 MUjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2n4RWlEPTB;M{KuPFczKM7:TR?= Mk\HV2FPT0WU
SW684 NFPuXY5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV;JR|UxRTN2LkG1OlEh|ryP M4P3XnNCVkeHUh?=
SK-MEL-28 MorXS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M13sfmlEPTB;M{WuOVM4PyEQvF2= M3jEZXNCVkeHUh?=
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P31-FUJ MmWxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3nLeWlEPTB;NESuN|I5PCEQvF2= M3Tp[XNCVkeHUh?=
NCI-H524 NV7XZYZOT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1PvV2lEPTB;NEiuPFI1QCEQvF2= M{\sfHNCVkeHUh?=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
POU5F1 / SOX2 / NANOG; 

PubMed: 27929731     


NT2/D1 cells treated with 10 nM of doxorubicin were subjected to WB analysis for pluripotency factors (POU5F1, NANOG, SOX2) and differentiation markers (TUBB3).

ATG5 / ATG7 / SQSTM1 / LC3A-II / LC3B-II / p-mTOR; 

PubMed: 27929731     


NT2/D1 cells treated with 10 nM of doxorubicin were subjected to WB analysis for autophagy markers (ATG5, ATG7, SQSTM1, LC3A-II, LC3B-II and p-MTOR). 

P62 / LC3II; 

PubMed: 28208617     


(A,B) Doxorubicin induces autophagy in pancreatic cancer cells. Western blot analysis of LC3-II and p62 protein levels in Mia PaCa-2 and Panc-1 cell lines treated with indicated concentrations of doxorubicin for 24 h, or treated with 2.5 μM doxorubicin for indicated times.

p-AKT(T308) / p-Akt(S473) / Akt / p-PRAS40(T246) / PRAS40 / p-H2A.X(S139); 

PubMed: 26027929     


MCF10A cells were serum-starved and treated with (A) 2 μM doxorubicin over a 10 hour time course.

27929731 28208617 26027929
Growth inhibition assay
Cell viability; 

PubMed: 25871400     


Salinomycin enhances the cytotoxicity of doxorubicin in HCC cellsRelative cell viability (mean ± SD) for salinomycin (black), doxorubicin (blue) and doxorubicin plus salinomycin (red) in HuH-7, HepG2, SNU-449 and SNU-387 cells.

25871400
Immunofluorescence
LC3; 

PubMed: 24970805     


Doxorubicin induces GFP-LC3 puncta formation in hepatocellular carcinoma cells. SK-Hep1 cells were treated with doxorubicin (2.5 μM) or CQ (10 μM) plus doxorubicin for 7 h and cells were examined with a confocal microscope for GFP-LC3 punctuation/aggregation.

24970805
ELISA
OPN; 

PubMed: 24963635     


A549 were exposed to 50 nM doxorubicin or PBS for 48 h. OPN concentrations in cell culture media were determined by ELISA.

24963635
In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
- Collapse
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (172.41 mM)
Water 20 mg/mL (34.48 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl

CAS No. 25316-40-9
Storage powder
in solvent
Synonyms NSC 123127, DOX
Smiles CC1C(C(CC(O1)OC2CC(CC3=C2C(=C4C(=C3O)C(=O)C5=C(C4=O)C(=CC=C5)OC)O)(C(=O)CO)O)N)O.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Step 2: Enter the in vivo formulation ()
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04423913 Not yet recruiting Other: Administration of embolization beads Prostate Cancer Centre Hospitalier Universitaire de Nīmes December 1 2020 Phase 2
NCT04204941 Recruiting Drug: Tazemetostat|Drug: Placebo|Drug: Doxorubicin HCl Advanced Soft Tissue Sarcoma|Advanced Epithelioid Sarcoma Epizyme Inc. December 19 2019 Phase 3
NCT04032964 Not yet recruiting Drug: L19TNF|Drug: DOXORUBICIN Soft Tissue Sarcoma Philogen S.p.A. September 2019 Phase 1
NCT03975205 Not yet recruiting Drug: Doxorubicin Lymphoma Leukemia Sutphin Drugs|Bharath Charitable Cancer Hospital and Institute July 1 2019 Early Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    Why the IC50 of the compound varied over time in our measurement?

  • Answer:

    Sometimes the IC50 value might vary between different batch of compounds due to the slight differences in purity. If different IC50 was seen in the same batch of compound, the problem may lies in the process of dissolvement. Make sure that you see no particles and the solution is clear with your stock solution.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID