Doxorubicin (Adriamycin) HCl

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127

Doxorubicin (Adriamycin) HCl Chemical Structure

Molecular Weight(MW): 579.98

Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.

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Cited by 46 Publications

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Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.
Targets
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
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H69 M{Ptc2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2\LPWlEPTB;MD64O{DPxE1? MmLiNVQ4QTV6NR?=
N417 MlnSS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfzUlFKSzVyPUCuNlMh|ryP NH7KN5oyPDd7NUi1
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T-MT4 NEHDRnBIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MVXJSFUxRTBwMEGg{txO NGHRSmcyPTR6Nkix
SKMEL-5 MonzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MUHFSFUxRTNwMjFOwG0> NV\veGZXOTZzM{e1Ny=>
MLM NVPOcJd1S3m2b4TvfIlkKEG|c3H5 NHj2T4tGTDVyPUCuNVMh|ryP NFv6bIEyQDd3M{Ww
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HT-29 MlPNS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFOyepExNjBzIN88[y=> NV;zO4pJPDhiaB?= MkHVRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdIm= M4rKN|I4Pzh2NEm=
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MCF M3XBNGZ2dmO2aX;uJGF{e2G7 NFftV3lT\X[ncoPhcEBw\iCSLXfwMY1m\GmjdHXkJI12dHSrZIL1[{Bz\XOrc4ThcoNmKGG|c3Xzd4VlKGG|IHXubIFv[2WvZX70JIlvKGO7dH;0c5hq[2m2eTDv[kBi\HKrYX35Z4lv MkTBPFk5PDF3MR?=
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C6 MlvFSpVv[3Srb36gRZN{[Xl? MlXzNk42KG2pL33M NWHNcIZjPCCq NVfxToE3TE2VTx?= MVTJcohq[mm2aX;uJI9nKHS3YoXsbY4heG:ueX3ldol7[XSrb36= NWHLW402QDl6OE[wOC=>
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SKVLB Mk\oR5l1d3SxeHnjJGF{e2G7 MWm3NkBp MoTuTWM2OD14LkW0JO69VQ>? MXO5NFA{PTJy
MCF7/VP16 MWTDfZRwfG:6aXOgRZN{[Xl? MXm1NEDPxGdxbVy= MoqxO|IhcA>? MlnQTWM2OD1zND6yJO69VQ>? NYK5[lVxQTB{Mke5Ni=>
CA755 NX7udJpLT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NV;CdZh6OTBizszN NVfQfW9uPiCm NVXr[ZlUUUN3ME2wMlAyPCEQvF2= MXG5OVI3PTdy
CHO NF\OcohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4n6U|ExKM7:TR?= M4TXN|Yh\A>? M4LpfWlEPTB;MD6wOkDPxE1? NX7ZdlJXQTV{NkW3NC=>
Panc02 NYnqT2ZpT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mor5NVAh|ryP MYG2JIQ> NF61VIVKSzVyPUCuNFY2KM7:TR?= MVq5OVI3PTdy
PC14 M3Ox[2N6fG:2b4jpZ{BCe3OjeR?= MVq0JIQ> M{fWNmROW09? NH;MXoxKSzVyPUCuNlkh|ryP NFf4[lQ6QDJ{NUSy
HOP62 MUTDfZRwfG:6aXOgRZN{[Xl? NEPwfoQ4OiCq NF7mTZhKSzVyPUCuNFA1KM7:TR?= MnfZPVg4OzZ4MR?=
SF-295 Mlf1R5l1d3SxeHnjJGF{e2G7 NHjkZZU4OiCq NVrkVWwxUUN3ME2wMlA{PSEQvF2= NITLW5I6QDd|Nk[x
MDA-MB-361 MYHDfZRwfG:6aXOgRZN{[Xl? NV\Y[5UyOSCq MkTlSG1UVw>? MmO4R5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDLV3CMVM3OSCneIDy[ZN{cW6pIFzhZ3ohf2m2aDDJR|UxKG:oIECuNFEzKM7:TR?= M2rTbVExOzl3NEmw
MDA-MB-361 NWDGOlBDS3m2b4TvfIlkKEG|c3H5 NVvD[Jc4OSCq MmHuSG1UVw>? MWXDfZRwfG:6aXPpeJkh[WejaX7zeEBOTEFiTVKgN|YyKGW6cILld5NqdmdiY3HyZo95gXCncITp[IF{\SC|dFPQS|Ihf2m2aDDJR|UxKG:oIECuNFE5KM7:TR?= M1flPFExOzl3NEmw
CCRF-SB MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV;GfmhOPCCm NHjEZZpFVVOR NGWzfXpKSzVyPUCuNFEh|ryP MkTNNVA1OTF2N{[=
CHO-K1 M{HtTGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MnfTOEBl MnS5SG1UVw>? M1jBUmlEPTB;MD60JO69VQ>? M1jzeFExPDFzNEe2
KB MlXRS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MU[0JIQ> M2e1W2ROW09? MYnBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuEN1Sge4l1cCCLQ{WwJI9nKDBwNkWg{txO M2\YcVExPDFzNEe2
KB M{K3Tmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml7QOEBl NV3LTYJHTE2VTx?= MmG5RY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDkdpVoNXKnc3nzeIFvfCCNQlPhcZB4cXSqIFnDOVAhd2ZiMD6wOkDPxE1? Mnm5NVA1OTF2N{[=
KB NVXPXlVVT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NVn6fY1mPCCm NXu0e454TE2VTx?= MVvBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuETVTSJJdqfGhiSVO1NEBw\iByLkWg{txO NYTlbmhROTB2MUG0O|Y>
KB NUTmW4h7T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1fjWVQh\A>? NFqyclJFVVOR NUfpUWxFSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRnYzOENid3n0bEBKSzVyIH;mJFAvPCEQvF2= MlPPNVA1OTF2N{[=
HTB-54 Mn;pR5l1d3SxeHnjJGF{e2G7 MlGyNVAxKM7:TR?= M3jmRWlEPTB;MT6xJO69VQ>? MoTnNVA1PzZ6NkG=
MEL-AC NH3nSoREgXSxdH;4bYMhSXO|YYm= NUL0WHRCOTByIN88US=> MmHJTWM2OD1zLkKg{txO NWXue|ZnOTB2N{[4OlE>
DU145 NHKwPWpEgXSxdH;4bYMhSXO|YYm= NFPve|M4OiCq MljVTWM2OD1yLkC1Nlgh|ryP NVvYeZZIOTB2N{myPFI>
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NCI-H226 NFf4OpVEgXSxdH;4bYMhSXO|YYm= Mn7BNVAxKM7:TR?= M2nZbFQ5KGh? MVzTZYxqdmV? NUTXUFFCS3m2b4TvfIlkcXS7IHHnZYlve3RiTlPJMWgzOjZiY3XscEApdXW2YX70JJA2Oylid3n0bEBKSzVyIH;mJFAvODB6IN88US=> MWqxNFc5ODlzMx?=
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A704 M1v5OWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3zFc2lEPTB;Mj6yPVE{PSEQvF2= M3HPfnNCVkeHUh?=
EVSA-T Mk\2S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1rqRWlEPTB;Mj6zNFA5QSEQvF2= M1jvOXNCVkeHUh?=
HCT-15 MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3HJU2lEPTB;Mj6zOVQ3PiEQvF2= MlLqV2FPT0WU
HuO-3N1 NFTQU4lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmPkTWM2OD1{LkO2OVg{KM7:TR?= NWrUbIJYW0GQR1XS
GMS-10 MWfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MVLJR|UxRTJwM{m3N|gh|ryP NF:1NYVUSU6JRWK=
TGBC24TKB NGDLRphIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M3nieWlEPTB;Mj60NVQzQSEQvF2= NHnQNXhUSU6JRWK=
C2BBe1 MmXPS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NV\Xb|N{UUN3ME2yMlUyOzJ6IN88US=> MVHTRW5ITVJ?
SK-N-DZ M3PtXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkDITWM2OD1{LkW2OVkzKM7:TR?= NXz3fIR7W0GQR1XS
SK-N-AS MmjrS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MmP1TWM2OD1{LkezNFE2KM7:TR?= NWLXZmx4W0GQR1XS
T98G MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHLWZo9KSzVyPUKuPFA1ODFizszN NVzJPGx7W0GQR1XS
DMS-79 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M3y5NmlEPTB;Mj65NFk5QCEQvF2= NWK3O28yW0GQR1XS
SNU-475 NG\LRppIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHzwNmZKSzVyPUKuPVI2PzJizszN Mn3JV2FPT0WU
COLO-792 M4XZZWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXLJR|UxRTNwMEK4O{DPxE1? M2rQXXNCVkeHUh?=
YAPC MnjjS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3zXXWlEPTB;Mz6zNlg{OiEQvF2= NVrKZoZpW0GQR1XS
UACC-812 M1O3XWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWfJR|UxRTNwM{O3N|gh|ryP NWe4eI1oW0GQR1XS
Mewo M{\qdWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MYfJR|UxRTNwNE[wPFYh|ryP NETHPZBUSU6JRWK=
COLO-824 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M{XUOGlEPTB;Mz61O|M4QCEQvF2= MWnTRW5ITVJ?
SCC-9 Moi0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NELz[5RKSzVyPUOuPVE{ODlizszN M{HjWnNCVkeHUh?=
CP50-MEL-B NUHJV4dGT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NGfPRXVKSzVyPUOuPVg4ODNizszN NU\EWmpyW0GQR1XS
NCI-H345 NXLySYxrT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mnv4TWM2OD12LkCxNVc1KM7:TR?= MV3TRW5ITVJ?
COR-L88 M1PQN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 Ml7pTWM2OD12LkO5OVgh|ryP NXqyN5lpW0GQR1XS
RCM-1 M1jifWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIrmUFZKSzVyPUSuOFkzPzJizszN MYDTRW5ITVJ?
JVM-3 NF\EOoFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1THZ2lEPTB;ND62NFYyQCEQvF2= NFmycXdUSU6JRWK=
ECC4 MYrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M37EXWlEPTB;ND63OlU1OSEQvF2= MYnTRW5ITVJ?
MDA-MB-453 M4TaR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3yxPWlEPTB;ND64OVE{PCEQvF2= MXvTRW5ITVJ?
SK-N-FI NYTofXI2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MW\JR|UxRTVwMkK4O|ch|ryP NELv[GhUSU6JRWK=
D-502MG MULHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWDJR|UxRTVwMkSyOlIh|ryP NFj3UnFUSU6JRWK=
NCI-H2227 M2LIXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFvVfGVKSzVyPUWuN|YzOzFizszN M1S1[nNCVkeHUh?=
DMS-53 Mmm1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGfBeo5KSzVyPU[uNVcyODZizszN MlHhV2FPT0WU
LB771-HNC NE\mXYVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1PDT2lEPTB;Nj60OlgxPiEQvF2= MX;TRW5ITVJ?
BB49-HNC MXnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUjJR|UxRTdwNEG3PVEh|ryP NWnKcJJxW0GQR1XS
MDA-MB-415 NEHRRYhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1z3O2lEPTB;Nz61O|MxOyEQvF2= NVrzRWxDW0GQR1XS
TALL-1 Mnf5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGSwcZFKSzVyPUeuPVY5QCEQvF2= MUTTRW5ITVJ?
HCC1395 NGDm[FhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MoC3TWM2OD16LkG1NVIzKM7:TR?= NVXLOY5TW0GQR1XS
EFE-184 M13mZ2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYCwN4tiUUN3ME24MlM4OjF4IN88US=> NWHrRod6W0GQR1XS
EC-GI-10 M1jaOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVfG[4g5UUN3ME24MlY5Pjh4IN88US=> NYfTZoRsW0GQR1XS
TT M2\tOWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NVHQcWN7UUN3ME25MlA3QTZ4IN88US=> M3GwcnNCVkeHUh?=
U-2-OS M1LUemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MVHJR|UxRTlwOEGzPVMh|ryP NWrocpB4W0GQR1XS
SHP-77 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGDXWZlKSzVyPUmuPVAzQSEQvF2= M2D3UHNCVkeHUh?=
HCC1143 MkjmS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVTJR|UxRTFyLkS3PVch|ryP MXjTRW5ITVJ?
A498 NYjpeod3T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{\kbWlEPTB;MUiuNlk4PiEQvF2= NYPxcWVDW0GQR1XS
LS-513 M4fVPGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlHvTWM2OD1zOD62NFk{KM7:TR?= NIfRVWtUSU6JRWK=
K052 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoGwTWM2OD1{MT60OFE5KM7:TR?= MWLTRW5ITVJ?
SiHa NVPuNlRxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUT3[nZ5UUN3ME2yN{4{OzdzIN88US=> MVnTRW5ITVJ?
RCC10RGB NE\ncHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2jPb2lEPTB;M{CuNFYyOSEQvF2= NH\1OmZUSU6JRWK=
NCI-SNU-16 Mn\LS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLoTWM2OD1|MT6xPFM3KM7:TR?= NFTr[FRUSU6JRWK=
SNU-C1 NVnkc4MyT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MmfLTWM2OD1|Mj60NVc2KM7:TR?= NGH5TmdUSU6JRWK=
C32 Mnv5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVnJR|UxRTN{Lki3NkDPxE1? M2Tab3NCVkeHUh?=
SW684 NH3WSJRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHnqUVlKSzVyPUO0MlE2PjFizszN NYDudVNJW0GQR1XS
SK-MEL-28 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWPCUW5ZUUN3ME2zOU42Ozd5IN88US=> MmraV2FPT0WU
SK-MEL-1 M1[ySWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1H3b2lEPTB;M{[uO|U1PyEQvF2= NGDvclFUSU6JRWK=
NCI-H720 NFexcFhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MULJR|UxRTRzLkm3NlYh|ryP M1XvcHNCVkeHUh?=
P31-FUJ MoDGS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M3P5cmlEPTB;NESuN|I5PCEQvF2= NIDzU3pUSU6JRWK=
NCI-H524 NWK5TmpKT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHTBWnBKSzVyPUS4MlgzPDhizszN MnXpV2FPT0WU

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
POU5F1 / SOX2 / NANOG; 

PubMed: 27929731     


NT2/D1 cells treated with 10 nM of doxorubicin were subjected to WB analysis for pluripotency factors (POU5F1, NANOG, SOX2) and differentiation markers (TUBB3).

ATG5 / ATG7 / SQSTM1 / LC3A-II / LC3B-II / p-mTOR; 

PubMed: 27929731     


NT2/D1 cells treated with 10 nM of doxorubicin were subjected to WB analysis for autophagy markers (ATG5, ATG7, SQSTM1, LC3A-II, LC3B-II and p-MTOR). 

P62 / LC3II; 

PubMed: 28208617     


(A,B) Doxorubicin induces autophagy in pancreatic cancer cells. Western blot analysis of LC3-II and p62 protein levels in Mia PaCa-2 and Panc-1 cell lines treated with indicated concentrations of doxorubicin for 24 h, or treated with 2.5 μM doxorubicin for indicated times.

p-AKT(T308) / p-Akt(S473) / Akt / p-PRAS40(T246) / PRAS40 / p-H2A.X(S139); 

PubMed: 26027929     


MCF10A cells were serum-starved and treated with (A) 2 μM doxorubicin over a 10 hour time course.

27929731 28208617 26027929
Growth inhibition assay
Cell viability; 

PubMed: 25871400     


Salinomycin enhances the cytotoxicity of doxorubicin in HCC cellsRelative cell viability (mean ± SD) for salinomycin (black), doxorubicin (blue) and doxorubicin plus salinomycin (red) in HuH-7, HepG2, SNU-449 and SNU-387 cells.

25871400
Immunofluorescence
LC3; 

PubMed: 24970805     


Doxorubicin induces GFP-LC3 puncta formation in hepatocellular carcinoma cells. SK-Hep1 cells were treated with doxorubicin (2.5 μM) or CQ (10 μM) plus doxorubicin for 7 h and cells were examined with a confocal microscope for GFP-LC3 punctuation/aggregation.

24970805
ELISA
OPN; 

PubMed: 24963635     


A549 were exposed to 50 nM doxorubicin or PBS for 48 h. OPN concentrations in cell culture media were determined by ELISA.

24963635
In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (172.41 mM)
Water 20 mg/mL (34.48 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl

CAS No. 25316-40-9
Storage powder
in solvent
Synonyms NSC 123127

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03571321 Not yet recruiting Acute Lymphoblastic Leukemia|ALL Childhood|ALL University of Chicago|Incyte Corporation September 5 2019 Phase 1
NCT03571321 Not yet recruiting Acute Lymphoblastic Leukemia|ALL Childhood|ALL University of Chicago|Incyte Corporation September 5 2019 Phase 1
NCT03108300 Not yet recruiting Malignant Soft Tissue Sarcoma Ain Shams University August 30 2019 Phase 2
NCT03108300 Not yet recruiting Malignant Soft Tissue Sarcoma Ain Shams University August 30 2019 Phase 2
NCT03437070 Withdrawn Leiomyosarcoma University of Miami|Janssen Scientific Affairs LLC June 2019 Phase 1
NCT03349281 Recruiting Refractory Acute Lymphoblastic Leukemia|Relapsed Acute Lymphoblastic Leukemia Julio Barredo|Takeda|University of Miami June 2019 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Why the IC50 of the compound varied over time in our measurement?

  • Answer:

    Sometimes the IC50 value might vary between different batch of compounds due to the slight differences in purity. If different IC50 was seen in the same batch of compound, the problem may lies in the process of dissolvement. Make sure that you see no particles and the solution is clear with your stock solution.

Topoisomerase Signaling Pathway Map

Related Topoisomerase Products0

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID