Doxorubicin (Adriamycin) HCl

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127

Doxorubicin (Adriamycin) HCl Chemical Structure

Molecular Weight(MW): 579.98

Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.

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Cited by 33 Publications

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  • Enhancement of apoptotic response by addition of targeted agents lapatinib and sunitinib to doxorubicin in the same device reservoir at 24 hours. P values were determined using Student’s t test for all graphs.

    Sci Transl Med, 2015, 7(284): 284ra57 . Doxorubicin (Adriamycin) HCl purchased from Selleck.

    (F) Kaplan-Meier curves are shown for all groups described: no hydrogel, a hydrogel loaded with paclitaxel, or a hydrogel loaded with doxorubicin. The number of mice per group (n) and median survival (ms) are listed. The experiment was performed at least three times.

    Science, 2018, 10(433), doi: 10.1126/scitranslmed.aar1916. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • Cell viabilities with increasing concentrations of cisplatin (CP) and doxorubicin (DOXO) under normoxic and hypoxic condition for 48 hours were determined by MTT assay. IC50 values are presented as the means ?SDs (n=4) and * denotes p<0.05.

    Cancer Res 2014 74, 298-308. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    Cleavage of caspase-3, -9 and the expression of PARP were measured by western blot using b-actin as a loading control.

    Cell Cycle, 2017, 16(5):468-476. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • (C) & (D) Treatments with doxorubicin, cisplatin or AZD6244 further decreased the cell viability in RH30 cloned cells in which STAT3 or GP130 was knockdown by shRNA. (*P<.05, ** P <.01, *** P <.001).

    Curr Cancer Drug Targets, 2016, 16(7):631-8. Doxorubicin (Adriamycin) HCl purchased from Selleck.

     

    Doxorubicin release of P(MAA-g-EG) and P(MAA-g-EG) containing nanoparticles in two-step pH conditions. Doxorubicinloaded P(MAA-g-EG) ,P(MAA-g-EG)-1.0NP ,P(MAA-g-EG)-2.5NP, and P(MAA-g-EG)-5.0NP crushed particles (75–150 um) were released in 1 PBS (pH 2.0) for 90 min. Then the pH was increased to 7.0 by adding 5N NaOH and release continued for 6 h.

    J Biomed Mater Res Part A 2012 10.1002/jbm.a.34064. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • Temporal uptake of free DOX or DOX-PCL63-b-PNVP90 micelles into K-562 (I) and DL (J) cells were studied by incubating in 24-well plates at a concentration of 50,000 cells per well. Representative images shown were obtained using a fluorescence microscope Eclipse 80i (Nikon, Japan) (Plan Fluor, 40X, NA 0.75 objective) equipped with blue and red filters for, Hoechst and Doxorubicin respectively (n = 3).

    PLoS One 2014 9, e94309. Doxorubicin (Adriamycin) HCl purchased from Selleck.

    PLoS One 2013 8(1), e54595. Doxorubicin (Adriamycin) HCl purchased from Selleck.

  • CON+ -positive control (with FBS as chemoattractant); CON- - negative control (without chemoattractant); DOX -doxorubicin; WZ -WZ811; PF- PF-573228; * -significant difference compared to positive control: p<0.05; ** -significant difference compared to positive control: p<0.01; # -significant difference between combination and inhibitor only: p<0.05. Cancer cell invasion was analyzed using Transwell membranes assembled in 24-well plates and covered with a layer of Matrigel. COR-L23 cells were treated for 24 h with 50 nM DOX, 10 uM WZ811, 5 uM PF-573228 and combinations of inhibitors with 50 nM DOX. Similarly, RH460 cells were treated for 24 h with 1 uM DOX, 10 uM WZ811, 2.5 uM PF-573228 and combinations of inhibitors with 1 uM DOX. Applied doses were the ones that exerted the most pronounced synergistic effect on cytotoxicity level. After the incubation period, cells that invaded through matrigel and membranes were fixed in 4% paraformaldehyde-PBS and stained with Hoechst 33342 nuclear stain. Cells were visualized and counted under a fluorescent microscope at 10x magnification.

    2014 Dr Milica Pesic from Institute for Biological Research. Doxorubicin (Adriamycin) HCl purchased from Selleck.

Purity & Quality Control

Choose Selective Topoisomerase Inhibitors

Biological Activity

Description Doxorubicin (Adriamycin) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage and apoptosis in tumor cells.
Targets
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
L1210 M2e2OGZ2dmO2aX;uJGF{e2G7 M2G3V2lvcGmkaYTpc44hd2ZiUl7BJJN6dnSqZYPpd{B4cXSqIFXEOVAhd2ZiMD61PEDPxE1? M320fFQ6ODV|Nh?=
Yoshida sarcoma NILJUFZHfW6ldHnvckBCe3OjeR?= M3;vO2lvcGmkaYTpc44hd2ZiW{PIYXRlWiCrbnPvdpBwemG2aX;uJJdqfGhiSVO1NEBw\iByLkCyJO69VQ>? NXGxbXlrOTN{Mkm4Oi=>
CHO NHezdW5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MnP4TWM2OD1yLkC2JO69VQ>? NYL2dIdXOTN3NEe1NC=>
8226 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MWrJR|UxRTBwMEOzJO69VQ>? NXjMRXJoOTR2N{ezNC=>
8226/ADR MnXCS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVLVTZhVUUN3ME2wMlU3KM7:TR?= NITuSYwyPDR5N{Ow
MCF7/ADR NYX5[XExT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnW1TWM2OD1|LkGg{txO MWmxOFQ4PzNy
H69 MkfFS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1\MSmlEPTB;MD64O{DPxE1? MUmxOFc6PTh3
N417 MV;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPNPW1zUUN3ME2wMlI{KM7:TR?= NH6zeGUyPDd7NUi1
H9 NHj3TVFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NI[4SYFKdmirYnn0bY9vKG:oIFi5JINmdGy|IHPodo9vcWOjbHz5JIlv\mWldHXkJJdqfGhidHjlJGhKXi1zIDjIPU9KUUmEKTD3bZRpKEmGNUCgc4YhOC5yMTFOwG0> NXHRUWx3OTV2OE[4NS=>
T-C8166 MmjES5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFrFZppKTDVyPUCuNFEh|ryP NGnGNZYyPTR6Nkix
T-H9 NGTlSYRIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NHG2TWJKTDVyPUCuNFEh|ryP M2fEfFE2PDh4OEG=
T-MT4 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXvJSFUxRTBwMEGg{txO M13qd|E2PDh4OEG=
SKMEL-5 M1qyW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfHR5RZTUR3ME2zMlIh|ryP NYf1XY9tOTZzM{e1Ny=>
MLM MXrDfZRwfG:6aXOgRZN{[Xl? MniwSWQ2OD1yLkGzJO69VQ>? NXi3T3BjOTh5NUO1NC=>
KBM-3 M1j2Umdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MXjJR|UxRTBwMEK2JO69VQ>? NVm4VGNjOTl7NUi3Oy=>
HT-29 NEjTTJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWWwMlAyKM7:Zx?= NETmZpQ1QCCq NHr6OnFCdnSrcILvcIln\XKjdHn2[UBi[3Srdnn0fS=> NIHNVXMzPzd6NES5
EMT6 MW\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYOwMlUh|ryP M4rtU|EhcA>? NHXQcpBKdmirYnn0bY9vKG:oIIDyc4xq\mW{YYTpc44h[nlibXXhd5VzcW6pIH\vdkB{fXK4aY\pcoch\nKjY4Tpc45{KHerdHigSWQ2OCCxZjCwMlAxODFizszN M33IcFc1PTJ4N{S=
H2981 M1TYUGN6fG:2b4jpZ{BCe3OjeR?= MkOyTWM2OD1yLkWg{txO MnjLO|c{OTB{Mx?=
LOX M13MPGN6fG:2b4jpZ{BCe3OjeR?= NFzzc|hKSzVyPUCuNFQzKM7:TR?= NFTBW4I4QDV|M{Ox
C38 Ml61R5l1d3SxeHnjJGF{e2G7 M3;wT2lEPTB;MD6wNUDPxE1? MlTlPFA2PzJ7MR?=
EAC MV;DfZRwfG:6aXOgRZN{[Xl? M1P1TlczKGh? MonpTWM2OD1yLkO1JO69VQ>? M2q3XVg{PTByOEe=
SKVLB MkHhR5l1d3SxeHnjJGF{e2G7 NXLpc3BxUUN3ME21MlYh|ryP MX64OVc3QTF2
AUC375 NYPtSnNqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHz6Z4JKSzVyPUCuNVEzKM7:TR?= NHjSdZY5PjR6NkCw
A2780-C25 Mm\zR5l1d3SxeHnjJGF{e2G7 NGLJeZNEgXSxdH;4bYNqfHliYXfhbY5{fCCxeHHsbZBt[XSrbj3y[ZNqe3SjboSgRVI4QDBvQ{K1JJdqfGhiSVO1NEBw\iByLkC2N{DPxE1? M3\oTFg5OzF5NUW=
A2780-CP3 M2LFc2N6fG:2b4jpZ{BCe3OjeR?= M3PmbGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGOrc4DsZZRqdi2{ZYPpd5RidnRiQUK3PFAuS1B|IIfpeIghUUN3MDDv[kAxNjB3NjFOwG0> M4rLSlg5OzF5NUW=
A2780-DX5 NX7jeGRTS3m2b4TvfIlkKEG|c3H5 NYT3e5pCS3m2b4TvfIlkcXS7IHHnZYlve3RiZH;4c5J2[mmlaX6tdoV{cXO2YX70JGEzPzhyLVTYOUB4cXSqIFnDOVAhd2ZiMD6zOVch|ryP NX7m[mRlQDh|MUe1OS=>
MXF7 M2HrVGN6fG:2b4jpZ{BCe3OjeR?= MknDRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4Sgd4Vve2m2aY\lJG1ZTjdid3n0bEBKSzVyIH;mJFAvODJ6IN88US=> NEjFcog5QTZyNUW4
MXF7 M3TabGN6fG:2b4jpZ{BCe3OjeR?= NHrLfXJCdnSrdIXtc5Ih[WO2aY\peJkh[WejaX7zeEBucXSxeHHueJJwdmVvcnXzbZN1[W62IF3YSlchf2m2aDDJR|UxKG:oIECuNFI5KM7:TR?= NE\YOlI5QTZyNUW4
MXF7 MW\DfZRwfG:6aXOgRZN{[Xl? M17Wd2FvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIRwgG:{dXLpZ4lvNXKnc3nzeIFvfCCPWF[3JJdqfGhiSVO1NEBw\iBzLkG3NkDPxE1? M3TJOVg6PjB3NUi=
WiDr MXXDfZRwfG:6aXOgRZN{[Xl? MXzBZ5Rqfmm2eTDh[4FqdnO2IIPlcpNqfGm4ZTDXbWRzKGOnbHyge4l1cCCLQ{WwJI9nKDBwMEWzJO69VQ>? NULkVnVzQDl4MEW1PC=>
MCF M4qxZmZ2dmO2aX;uJGF{e2G7 NFjPdHhT\X[ncoPhcEBw\iCSLXfwMY1m\GmjdHXkJI12dHSrZIL1[{Bz\XOrc4ThcoNmKGG|c3Xzd4VlKGG|IHXubIFv[2WvZX70JIlvKGO7dH;0c5hq[2m2eTDv[kBi\HKrYX35Z4lv NFXTb245QTh2MUWx
C6 M3LNTGN6fG:2b4jpZ{BCe3OjeR?= MYeyMlUhdWdxbVy= MV23NkBp MU\EUXNQ M1;pXGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KHKjdDDDOkBk\WyuczDheEA2OCC3Zz;tUEB1dyB{LkWgcYcwdUx? M{TQSlg6QDh4MES=
C6 NXzPb5l5TnWwY4Tpc44hSXO|YYm= NETaN28zNjVibXevcWw> MVO0JIg> NFfYfIlFVVOR MlXNTY5pcWKrdHnvckBw\iC2dXL1cIlvKHCxbInt[ZJqgmG2aX;u MnXrPFk5QDZyNB?=
C6 MWLDfZRwfG:6aXOgRZN{[Xl? NYXSOXg{Oi53IH3nM41N MY[1JIg> NVHFNFZ6TE2VTx?= MX3DfZRwfG:6aXPpeJkh[XO|ZYPz[YQh[XNiY3XscEBz\WynYYPl M3\IUFg6QDh4MES=
SKVLB NYXo[HpYS3m2b4TvfIlkKEG|c3H5 MWe3NkBp NU\5bJU5UUN3ME22MlU1KM7:TR?= MlPKPVAxOzV{MB?=
MCF7/VP16 NHzEOppEgXSxdH;4bYMhSXO|YYm= NY\kfmd6PTBizsznM41N NF2xZW84OiCq NGmyNHZKSzVyPUG0MlIh|ryP NUDjfodWQTB{Mke5Ni=>
CA755 NEnFT2VIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MY[xNEDPxE1? NFvMV403KGR? MWnJR|UxRTBwMEG0JO69VQ>? M{HKdFk2OjZ3N{C=
CHO MlLUS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFTieIkyOCEQvF2= MWS2JIQ> MlLyTWM2OD1yLkC2JO69VQ>? M4DXZ|k2OjZ3N{C=
Panc02 NFfFS5JIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mo\pNVAh|ryP MnPNOkBl MWnJR|UxRTBwME[1JO69VQ>? MUC5OVI3PTdy
PC14 NWrPOplbS3m2b4TvfIlkKEG|c3H5 MnvROEBl M2DaeWROW09? MYXJR|UxRTBwMkmg{txO MmTaPVgzOjV2Mh?=
HOP62 NEfXN|dEgXSxdH;4bYMhSXO|YYm= Ml7LO|IhcA>? NYnUPXZPUUN3ME2wMlAxPCEQvF2= MWO5PFc{PjZz
SF-295 NUO4W2ZRS3m2b4TvfIlkKEG|c3H5 M1rQU|czKGh? NIHpXlVKSzVyPUCuNFM2KM7:TR?= M1ra[|k5PzN4NkG=
MDA-MB-361 NWTxV21TS3m2b4TvfIlkKEG|c3H5 NFTqeIgyKGh? MUPEUXNQ NUfiSXhsS3m2b4TvfIlkcXS7IHHnZYlve3RiTVTBMW1DNTN4MTDlfJBz\XO|aX7nJGxi[1pid3n0bEBKSzVyIH;mJFAvODF{IN88US=> NHvnfI0yODN7NUS5NC=>
MDA-MB-361 NFjHc2tEgXSxdH;4bYMhSXO|YYm= MUmxJIg> MmjESG1UVw>? M3\6TWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQTDNRkA{PjFiZYjwdoV{e2mwZzDjZZJjd3i7cHXweIll[XOnIIP0R3BIOiC5aYToJGlEPTBib3[gNE4xOThizszN Mln3NVA{QTV2OUC=
CCRF-SB MWjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVfpOFhOPCCm NG\LPWRFVVOR NX;rfmxFUUN3ME2wMlAyKM7:TR?= NIjoPI4yODRzMUS3Oi=>
CHO-K1 MXTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MX60JIQ> MoLySG1UVw>? MnTSTWM2OD1yLkSg{txO MnTTNVA1OTF2N{[=
KB M{fZOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MmXrOEBl NGL4cYVFVVOR NXrrUlF[SW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRldFKHerdHigTWM2OCCxZjCwMlY2KM7:TR?= M3ey[lExPDFzNEe2
KB MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXvwbpFPPCCm MoPLSG1UVw>? MkPGRY51cXC{b3zp[oVz[XSrdnWgZYN1cX[rdImgZYdicW6|dDDkdpVoNXKnc3nzeIFvfCCNQlPhcZB4cXSqIFnDOVAhd2ZiMD6wOkDPxE1? MUixNFQyOTR5Nh?=
KB M1PHW2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1f6ZlQh\A>? NYjwOoV1TE2VTx?= MYrBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuETVTSJJdqfGhiSVO1NEBw\iByLkWg{txO MnLvNVA1OTF2N{[=
KB NHzJUlNIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MlLCOEBl NU\Te|g{TE2VTx?= M13EVWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[JJ2\y2{ZYPpd5RidnRiS1LWNlBEKHerdHigTWM2OCCxZjCwMlQh|ryP NVPxWmV7OTB2MUG0O|Y>
HTB-54 NHfHUY1EgXSxdH;4bYMhSXO|YYm= NV3lPJhNOTByIN88US=> NYnORndIUUN3ME2xMlEh|ryP MXuxNFQ4Pjh4MR?=
MEL-AC M1K3WGN6fG:2b4jpZ{BCe3OjeR?= MXyxNFAh|ryP NYjleoREUUN3ME2xMlIh|ryP NYLiSJl7OTB2N{[4OlE>
DU145 NHvwO3NEgXSxdH;4bYMhSXO|YYm= MmTQO|IhcA>? NH\jWm9KSzVyPUCuNFUzQCEQvF2= MmXONVA1Pzl{OEK=
K562R NFruZmREgXSxdH;4bYMhSXO|YYm= NVjMW4t2PzJiaB?= NIT6N4dKSzVyPUCuNFE2PiEQvF2= NIr4WnAyODR5OUK4Ni=>
PACA-2 MYLDfZRwfG:6aXOgRZN{[Xl? M2XJUlYh\A>? M3;vOGVFPTB;MD6wNFY{KM7:TR?= MV:xNFQ6QDJzNB?=
3Y1 M2jvXWN6fG:2b4jpZ{BCe3OjeR?= MYm3NkBp M2jLNmdKPTB;MD6wNFM5KM7:TR?= MVuxNFY6OTdzMB?=
P388 MWnDfZRwfG:6aXOgRZN{[Xl? M1:zXVczKGh? M2TLXGdKPTB;MD6wNVIh|ryP NHizZXcyODZ7MUexNC=>
HMV-1 MYPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Mn24TWM2OD1yLkK4JO69VQ>? M{nEVlExPjl6NEO2
MKN-1 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGK2SWZKSzVyPUCuOlQh|ryP M3nySVExPjl6NEO2
PC14 MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2nyVWlEPTB;MT65JO69VQ>? M33QWlExPjl6NEO2
T24 NFrSdYtIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXPJR|UxRTBwNkSg{txO NWnyV|JXOTB4OUi0N|Y>
U937 MYXGeY5kfGmxbjDBd5NigQ>? NYSyR2k3OjRiaB?= NXXJboJJTE2VTx?= MoPvTY5pcWKrdIOgbY5kd3Kyb4LheIlwdiCxZjDbN2hefGi7bXnkbY5mKHerdHigTWM2OCCxZjCwMlEh|ryP M4nkdVExPzhyOUGx
H460pv8/eto Ml7zR5l1d3SxeHnjJGF{e2G7 NEjUWGYyODBizszN MmLIOFghcA>? NXvBZYljW2GuaX7l NYC5XlNCUUN3ME2wMlAzKM7:TR?= NVeybnpWOTB5OEC5NVM>
H460pv8 NUTremN4S3m2b4TvfIlkKEG|c3H5 NFnNdpcyODBizszN MoXNOFghcA>? M2PmVnNidGmwZR?= NUPiSphSUUN3ME2wMlAxPCEQvF2= MlvZNVA4QDB7MUO=
MDA-231 NXfXVXdlS3m2b4TvfIlkKEG|c3H5 MYOxNFAh|ryP NEe5XWg1QCCq NVLXVmtJW2GuaX7l M2fxTGN6fG:2b4jpZ4l1gSCjZ3HpcpN1KE2GQT2yN|Eh[2WubDCoSXIuNEWJRmKrMI12fGGwdDDwOVMqKHerdHigTWM2OCCxZjCwMlAxPiEQvF2= Mo[2NVA4QDB7MUO=
MDA-468 MmPMR5l1d3SxeHnjJGF{e2G7 MofXNVAxKM7:TR?= NHnXRmc1QCCq Mo\KV4FtcW6n MY\DfZRwfG:6aXPpeJkh[WejaX7zeEBOTEFvNE[4JINmdGxiKFXSMUwh[W2ybHnmbYVlKEWJRmKsJI12fGGwdDDwOVMqKHerdHigTWM2OCCxZjCwMlAxPSEQvF2= M2XXZlExPzhyOUGz
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Daudi Mn25R5l1d3SxeHnjJGF{e2G7 MV:xNFAh|ryP MlfuPVYhcA>? M2fZVWN6fG:2b4jpZ4l1gSCjZ3HpcpN1KGi3bXHuJGRifWSrIHPlcIx{KHWwZHXyJIRm\W1ibHnnbJQhf2m2aDDJR|UxKG:oIECuN{DPxE1? NGTofnUyPzl7M{K3OS=>
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HepG2 MXHDfZRwfG:6aXOgRZN{[Xl? NWnpOXZUOTByIN88US=> NHTHdIc4OiCq MWLJR|UxRTBwMEeg{txO M3LYUFE5OThzNUe1
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Colon 26-L5 MofPR5l1d3SxeHnjJGF{e2G7 NYrDN|k4PzJiaB?= NH3jTohFVVOR M4XVN2lEPTB;MD6wNUDPxE1? MVixPFQ1ODJ|Mx?=
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Bel7402 NIjIVo5EgXSxdH;4bYMhSXO|YYm= NFLLPXA4OiCq NG\ORoJFVVOR MmjjTWM2OD1yLkCyNUDPxE1? M{HrRVE6ODJ6NEK1
HT-29 MWfDfZRwfG:6aXOgRZN{[Xl? MYS3NkBp M3TWS2ROW09? MmjCTWM2OD1yLkCxPEDPxE1? MX:xPVAzQDR{NR?=
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Detroit562 MoryS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M172UGlEPTB;MkOwMlczKG6P M17jSnNCVkeHUh?=
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PC-14 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXzyOFlbUUN3ME2yN|YvOTJibl2= MVvTRW5ITVJ?
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D-392MG NVqweottT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M37GZ2lEPTB;MkS4MlUhdk1? NUnXeZFxW0GQR1XS
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Calu-6 MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFvk[|NKSzVyPUK1N{42PSCwTR?= NUnQVZFxW0GQR1XS
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NCI-H520 M2jte2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkTaTWM2OD1{Nk[uOlEhdk1? MnrqV2FPT0WU
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SW756 Mn\IS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWjJR|UxRTJ5ND63PEBvVQ>? MXLTRW5ITVJ?
NB12 NUTxV4dFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NIrMfmpKSzVyPUK3O{4yOiCwTR?= M4nGWXNCVkeHUh?=
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NCI-H23 NHfkbItIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NIfMXnVKSzVyPUK4N{46OiCwTR?= MXnTRW5ITVJ?
GOTO MmTHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFXPbXVKSzVyPUK4OU42PiCwTR?= NWCzW2RXW0GQR1XS
NCI-H1693 NHf3ZY1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\3SnpCUUN3ME2yPVAvOzJibl2= MVzTRW5ITVJ?
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MDA-MB-361 Ml;OS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MoW5TWM2OD1{OUKuOlUhdk1? M3PTcHNCVkeHUh?=
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CAL-54 MWXHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEi0NWtKSzVyPUOwNE44OyCwTR?= MXrTRW5ITVJ?
D-263MG Mmf6S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGnDVIFKSzVyPUOwOU42OiCwTR?= NVnPRWZ3W0GQR1XS
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NCI-H1304 MVTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M2TvO2lEPTB;M{KxMlE4KG6P MVHTRW5ITVJ?
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EM-2 MWLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmrFTWM2OD1|MkWuOFEhdk1? M1THNXNCVkeHUh?=
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EW-11 NHjhZ|FIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV30eHNpUUN3ME2zOFEvPTZibl2= Mn3rV2FPT0WU
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LU-139 MkTwS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXTORmFkUUN3ME2zOFQvQTJibl2= M{j3SHNCVkeHUh?=
U-87-MG MX;Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV7sVFV2UUN3ME2zOFYvODRibl2= M{XpT3NCVkeHUh?=
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SCC-9 M{TOR2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEOxfllKSzVyPUOuPVE{ODlizszN M4m3RnNCVkeHUh?=
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NCI-H345 NYHtT5MzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH3yVFVKSzVyPUSuNFEyPzRizszN MmLyV2FPT0WU
COR-L88 MoC4S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{LkTGlEPTB;ND6zPVU5KM7:TR?= NGjPNoxUSU6JRWK=
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JVM-3 MVrHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWrPTJFYUUN3ME20MlYxPjF6IN88US=> M{Ds[nNCVkeHUh?=
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D-502MG MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWfkU4g3UUN3ME21MlI1OjZ{IN88US=> MlHjV2FPT0WU
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DMS-53 M2S4dWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4TEPGlEPTB;Nj6xO|ExPiEQvF2= MoPkV2FPT0WU
LB771-HNC M3G4fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoHjTWM2OD14LkS2PFA3KM7:TR?= NHXre2NUSU6JRWK=
BB49-HNC MoDkS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MYjJR|UxRTdwNEG3PVEh|ryP NYW5R2pRW0GQR1XS
MDA-MB-415 MVLHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoLOTWM2OD15LkW3N|A{KM7:TR?= NWXrbmtYW0GQR1XS
TALL-1 M1rmbmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MoX3TWM2OD15Lkm2PFgh|ryP MUXTRW5ITVJ?
HCC1395 NInTXJhIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWHJR|UxRThwMUWxNlIh|ryP MlHpV2FPT0WU
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TT NH;H[HVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXvJR|UxRTlwME[5OlYh|ryP MmnNV2FPT0WU
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SiHa M3LGWGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NHHaSHZKSzVyPUKzMlM{PzFizszN M{K1RXNCVkeHUh?=
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NCI-SNU-16 NUP5[2wxT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2WxXWlEPTB;M{GuNVg{PiEQvF2= NIPheXBUSU6JRWK=
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C32 MnPVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYDob4ZOUUN3ME2zNk45PzJizszN MmGyV2FPT0WU
SW684 M3jPRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MUDJR|UxRTN2LkG1OlEh|ryP M1TNZnNCVkeHUh?=
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... Click to View More Cell Line Experimental Data

In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
+ Expand
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Formulation: Dissolved in DMSO, and diluted in saline
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (172.41 mM)
Water 20 mg/mL (34.48 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl

CAS No. 25316-40-9
Storage powder
in solvent
Synonyms NSC 123127

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
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Molecular Weight Calculator

Molecular Weight Calculator

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Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT03571321 Not yet recruiting Acute Lymphoblastic Leukemia|ALL Childhood|ALL University of Chicago|Incyte Corporation September 5 2019 Phase 1
NCT03108300 Not yet recruiting Malignant Soft Tissue Sarcoma Ain Shams University August 30 2019 Phase 2
NCT03742986 Not yet recruiting Breast Cancer New York University School of Medicine April 2019 Phase 2
NCT03349281 Recruiting Refractory Acute Lymphoblastic Leukemia|Relapsed Acute Lymphoblastic Leukemia Julio Barredo|Takeda|University of Miami March 2019 Phase 1
NCT03331341 Not yet recruiting Classical Hodgkin Lymphoma University of Washington|National Cancer Institute (NCI) February 15 2019 Phase 2
NCT03669783 Not yet recruiting Childhood Renal Tumor Assistance Publique Hopitaux De Marseille January 1 2019 Phase 3

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

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Frequently Asked Questions

  • Question 1:

    Why the IC50 of the compound varied over time in our measurement?

  • Answer:

    Sometimes the IC50 value might vary between different batch of compounds due to the slight differences in purity. If different IC50 was seen in the same batch of compound, the problem may lies in the process of dissolvement. Make sure that you see no particles and the solution is clear with your stock solution.

Topoisomerase Signaling Pathway Map

Related Topoisomerase Products0

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID