Doxorubicin (Adriamycin) HCl

For research use only.

Licensed by Pfizer Catalog No.S1208 Synonyms: NSC 123127, DOX

657 publications

Doxorubicin (Adriamycin) HCl Chemical Structure

Molecular Weight(MW): 579.98

Doxorubicin (Adriamycin, NSC 123127, DOX) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage, mitophagy and apoptosis in tumor cells. Doxorubicin reduces basal phosphorylation of AMPK. Doxorubicin is used in the concomitant treatment of HIV-infected patients but is found to be at high risk of HBV reactivation.

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Selleck's Doxorubicin (Adriamycin) HCl has been cited by 657 publications

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Choose Selective Antineoplastic and Immunosuppressive Antibiotics Inhibitors

Biological Activity

Description Doxorubicin (Adriamycin, NSC 123127, DOX) HCl is an antibiotic agent that inhibits DNA topoisomerase II and induces DNA damage, mitophagy and apoptosis in tumor cells. Doxorubicin reduces basal phosphorylation of AMPK. Doxorubicin is used in the concomitant treatment of HIV-infected patients but is found to be at high risk of HBV reactivation.
Targets
Topo II [1]
(Tumor cells)
In vitro

Doxorubicin, an antibiotic anthracycline, is commonly considered to exert its anti-tumor activity at two fundamental levels, altering DNA and producing free radicals to trigger apoptosis of cancer cells through DNA damage. Doxorubicin can block the synthesis of DNA by intercalating into the DNA strand, and inhibits DNA topoisomerase II (TOP2). Doxorubicin is most effective when cells are rapidly proliferating and expressing high levels of TOP2. Additionally, Doxorubicin can trigger apoptosis by producing ceramide (which prompts apoptosis by activating p53 or other downstream pathways such as JNK), the degradation of Akt by serine threonine proteases, the mitochondrial release of cytochrome c, increased FasL (death receptor Fas/CD95 ligand) mRNA production, and a greater production of free radicals. [2] Pre-treatment with GSNO (nitrosoglutathione) suppresses the resistance in the doxorubicin-resistant breast cancer cell line MCF7/Dx, accompanied by enhanced protein glutathionylation and accumulation of doxorubicin in the nucleus. [3] Doxorubicin induced G2/M checkpoint arrest are attributed to elevated cyclin G2 (CycG2) expression and phospho-modification of proteins in the ataxia telangiectasia mutated (ATM) and ATM and Rad3-related (ATR) signaling pathways. [5] Doxorubicin inhibits AMP-activated protein kinase (AMPK), resulting in SIRT1 dysfunction, p53 accumulation, and increased cell death in mouse embryonic fibroblasts (MEFs) and cardiomyocytes, which can be further sensitized by pre-inhibition of AMPK. [6] Doxorubicin elicits a marked heat shock response, and that either inhibition or silencing of heat shock proteins enhance the Doxorubicin apoptotic effect in neuroblastoma cells. Nanomolar Doxorubicin treatment of neuroblastoma cells causes dose-dependent over-ubiquitination of a specific set of proteins in the absence of measurable inhibition of proteasome, and loss of activity of ubiquitinated enzymes such as lactate dehydrogenase and α-enolase, the protein ubiquination patterns of which is similar to those with proteasome inhibitor Bortezomib, indicating that Doxorubicin may also exert its effect by damaging proteins. [8]

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
L1210 MV\GeY5kfGmxbjDBd5NigQ>? NHjGe3FKdmirYnn0bY9vKG:oIGLORUB{gW62aHXzbZMhf2m2aDDFSFUxKG:oIECuOVgh|ryP NUWwRWxsPDlyNUO2
Yoshida sarcoma M2q2bGZ2dmO2aX;uJGF{e2G7 M{OyUmlvcGmkaYTpc44hd2ZiW{PIYXRlWiCrbnPvdpBwemG2aX;uJJdqfGhiSVO1NEBw\iByLkCyJO69VQ>? M3TMNlE{OjJ7OE[=
CHO MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEnUWVFKSzVyPUCuNFYh|ryP MX[xN|U1PzVy
8226 Mo\yS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXO4XGdYUUN3ME2wMlA{OyEQvF2= NHT5OGYyPDR5N{Ow
8226/ADR MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M13VfWlEPTB;MD61OkDPxE1? M4LSO|E1PDd5M{C=
MCF7/ADR MnrDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NGX5TWNKSzVyPUOuNUDPxE1? NGDnWFUyPDR5N{Ow
H69 NUD1NVJ2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NUDZdYt[UUN3ME2wMlg4KM7:TR?= NITFflcyPDd7NUi1
N417 MY\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NV\zTHR3UUN3ME2wMlI{KM7:TR?= M1v5TlE1Pzl3OEW=
H9 MlLxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NWfjV5c4UW6qaXLpeIlwdiCxZjDIPUBk\WyuczDjbJJwdmmlYXzsfUBqdm[nY4Tl[EB4cXSqIITo[UBJUVZvMTCoTFkwUUmLQjmge4l1cCCLREWwJI9nKDBwMEGg{txO NYHaPHpMOTV2OE[4NS=>
T-C8166 NHLEfohIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWXKVXRlUUR3ME2wMlAyKM7:TR?= NYryb2d{OTV2OE[4NS=>
T-H9 M1jF[Gdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M1K4eGlFPTB;MD6wNUDPxE1? M3XBO|E2PDh4OEG=
T-MT4 M1;VNmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUPicYZMUUR3ME2wMlAyKM7:TR?= NF\zVnoyPTR6Nkix
SKMEL-5 M2rhbGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGS3d3RGTDVyPUOuNkDPxE1? M2jvR|E3OTN5NUO=
MLM Mn7ER5l1d3SxeHnjJGF{e2G7 MXvFSFUxRTBwMUOg{txO NEXlSIIyQDd3M{Ww
KBM-3 MYfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWr5NZRqUUN3ME2wMlAzPiEQvF2= NXT5TmM2OTl7NUi3Oy=>
HT-29 NIrodYpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NH7YfYIxNjBzIN88[y=> NU[5T5lDPDhiaB?= NYrndZUzSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJk> NHfj[m8zPzd6NES5
EMT6 MmrzS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH\rV2wxNjVizszN Ml3QNUBp NHPGeIxKdmirYnn0bY9vKG:oIIDyc4xq\mW{YYTpc44h[nlibXXhd5VzcW6pIH\vdkB{fXK4aY\pcoch\nKjY4Tpc45{KHerdHigSWQ2OCCxZjCwMlAxODFizszN M3T1bFc1PTJ4N{S=
H2981 M3XGVWN6fG:2b4jpZ{BCe3OjeR?= MlL2TWM2OD1yLkWg{txO MVW3O|MyODJ|
LOX MoLMR5l1d3SxeHnjJGF{e2G7 NFe4S2VKSzVyPUCuNFQzKM7:TR?= NGHibVY4QDV|M{Ox
C38 NH[xe4JEgXSxdH;4bYMhSXO|YYm= M4fKfmlEPTB;MD6wNUDPxE1? MX:4NFU4Ojlz
EAC MmruR5l1d3SxeHnjJGF{e2G7 NH;zXZU4OiCq M3XvbmlEPTB;MD6zOUDPxE1? M37teVg{PTByOEe=
SKVLB MoT4R5l1d3SxeHnjJGF{e2G7 NFrxPZZKSzVyPUWuOkDPxE1? MYm4OVc3QTF2
AUC375 NH3qfFFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MUHJR|UxRTBwMUGyJO69VQ>? M4X2Olg3PDh4MEC=
A2780-C25 NIm3PXREgXSxdH;4bYMhSXO|YYm= NXvxPVNxS3m2b4TvfIlkcXS7IHHnZYlve3Rib4jhcIlxdGG2aX6tdoV{cXO2YX70JGEzPzhyLVOyOUB4cXSqIFnDOVAhd2ZiMD6wOlMh|ryP M4fGVVg5OzF5NUW=
A2780-CP3 M2f2SmN6fG:2b4jpZ{BCe3OjeR?= Mnj1R5l1d3SxeHnjbZR6KGGpYXnud5Qh[2m|cHzheIlvNXKnc3nzeIFvfCCDMke4NE1EWDNid3n0bEBKSzVyIH;mJFAvODV4IN88US=> NIjiRmg5QDNzN{W1
A2780-DX5 NXj1SVR{S3m2b4TvfIlkKEG|c3H5 NGrYdZNEgXSxdH;4bYNqfHliYXfhbY5{fCCmb4jvdpVjcWOrbj3y[ZNqe3SjboSgRVI4QDBvRGi1JJdqfGhiSVO1NEBw\iByLkO1O{DPxE1? M{PlSVg5OzF5NUW=
MXF7 NU\sOHRGS3m2b4TvfIlkKEG|c3H5 M{LMS2FvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JJNmdnOrdHn2[UBOYEZ5IIfpeIghUUN3MDDv[kAxNjB{ODFOwG0> NI\5TlU5QTZyNUW4
MXF7 NWfhdFdTS3m2b4TvfIlkKEG|c3H5 MknvRY51cXS3bX;yJIFkfGm4aYT5JIFo[Wmwc4SgcYl1d3ijboTyc45mNXKnc3nzeIFvfCCPWF[3JJdqfGhiSVO1NEBw\iByLkCyPEDPxE1? NIHIbHQ5QTZyNUW4
MXF7 NYLaRnpXS3m2b4TvfIlkKEG|c3H5 M3XhdmFvfGm2dX3vdkBi[3Srdnn0fUBi\2GrboP0JIRwgG:{dXLpZ4lvNXKnc3nzeIFvfCCPWF[3JJdqfGhiSVO1NEBw\iBzLkG3NkDPxE1? NF7MSIo5QTZyNUW4
WiDr M332UmN6fG:2b4jpZ{BCe3OjeR?= M{LmR2FkfGm4aYT5JIFo[Wmwc4Sgd4Vve2m2aY\lJHdqTHJiY3XscEB4cXSqIFnDOVAhd2ZiMD6wOVMh|ryP M4PBWFg6PjB3NUi=
MCF MmPZSpVv[3Srb36gRZN{[Xl? MmniVoV3\XK|YXygc4YhWC2pcD3t[YRq[XSnZDDteYx1cWS{dXegdoV{cXO2YX7j[UBie3Onc4Pl[EBieyCnbnjhcoNmdWWwdDDpckBkgXSxdH;4bYNqfHlib3[gZYRzcWGveXPpci=> MWm4PVg1OTVz
C6 M4D1T2N6fG:2b4jpZ{BCe3OjeR?= NHvCOGIzNjVibXevcWw> Mn\4O|IhcA>? NXjuS|I1TE2VTx?= MUnDfZRwfG:6aXPpeJkh[WejaX7zeEBz[XRiQ{[gZ4VtdHNiYYSgOVAhfWdxbVygeI8hOi53IH3nM41N NWLROopXQDl6OE[wOC=>
C6 NV76XmhoTnWwY4Tpc44hSXO|YYm= NGXS[ZUzNjVibXevcWw> MXy0JIg> M3\CeGROW09? M{\KSGlvcGmkaYTpc44hd2ZidIXieYxqdiCyb3z5cYVzcXqjdHnvci=> NXSxbHlDQDl6OE[wOC=>
C6 MUXDfZRwfG:6aXOgRZN{[Xl? MV6yMlUhdWdxbVy= M{fXcVUhcA>? M4\aNmROW09? NHnCSo1EgXSxdH;4bYNqfHliYYPz[ZN{\WRiYYOgZ4VtdCC{ZXzlZZNm NEDGWI05QTh6NkC0
SKVLB Mle2R5l1d3SxeHnjJGF{e2G7 MW[3NkBp Moj5TWM2OD14LkW0JO69VQ>? Mk\FPVAxOzV{MB?=
MCF7/VP16 MVLDfZRwfG:6aXOgRZN{[Xl? M{DZVlUxKM7:Zz;tUC=> NVvCb4xlPzJiaB?= Mli5TWM2OD1zND6yJO69VQ>? NWi5TmlGQTB{Mke5Ni=>
CA755 NFLIZ2xIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWCxNEDPxE1? MnTSOkBl MoizTWM2OD1yLkCxOEDPxE1? M2nTe|k2OjZ3N{C=
CHO M{PyXWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NFW5UoEyOCEQvF2= MoK5OkBl Mn24TWM2OD1yLkC2JO69VQ>? NV33TIxpQTV{NkW3NC=>
Panc02 MU\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MkjkNVAh|ryP NYHxZ2Y2PiCm NI\qPYJKSzVyPUCuNFY2KM7:TR?= MVW5OVI3PTdy
PC14 NGe0VVhEgXSxdH;4bYMhSXO|YYm= NHzyfZA1KGR? MW\EUXNQ MkjKTWM2OD1yLkK5JO69VQ>? M2XBXFk5OjJ3NEK=
HOP62 M4XlTWN6fG:2b4jpZ{BCe3OjeR?= M{jvR|czKGh? NGTaOmpKSzVyPUCuNFA1KM7:TR?= NYLJTG5pQTh5M{[2NS=>
SF-295 M3i1SWN6fG:2b4jpZ{BCe3OjeR?= MWC3NkBp MnrvTWM2OD1yLkCzOUDPxE1? MnTJPVg4OzZ4MR?=
MDA-MB-361 MnXiR5l1d3SxeHnjJGF{e2G7 MWSxJIg> M1nFN2ROW09? MX3DfZRwfG:6aXPpeJkh[WejaX7zeEBOTEFvTVKtN|YyKGW6cILld5NqdmdiTHHjXkB4cXSqIFnDOVAhd2ZiMD6wNVIh|ryP MkXGNVA{QTV2OUC=
MDA-MB-361 NUiyWW84S3m2b4TvfIlkKEG|c3H5 M{fSbVEhcA>? Ml;oSG1UVw>? MlzJR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDIF3CJFM3OSCneIDy[ZN{cW6pIHPhdoJwgHmyZYD0bYRie2Vic4TDVGczKHerdHigTWM2OCCxZjCwMlAyQCEQvF2= MlmzNVA{QTV2OUC=
CCRF-SB MonVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MkHxOEBl NXnGTFd6TE2VTx?= MX7JR|UxRTBwMEGg{txO MlnPNVA1OTF2N{[=
CHO-K1 NFjZd4dIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MWq0JIQ> MULEUXNQ M1fvXWlEPTB;MD60JO69VQ>? NVqzfHpTOTB2MUG0O|Y>
KB MWnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NF;2cmc1KGR? MYnEUXNQ NYrKeZpkSW62aYDyc4xq\mW{YYTpeoUh[WO2aY\peJkh[WejaX7zeEBlenWpLYLld4l{fGGwdDDLRldFKHerdHigTWM2OCCxZjCwMlY2KM7:TR?= NH;XZ3MyODRzMUS3Oi=>
KB NXHIWlNST3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MoHiOEBl MWPEUXNQ M4qzSWFvfGmycn;sbYZmemG2aY\lJIFkfGm4aYT5JIFo[Wmwc4Sg[JJ2\y2{ZYPpd5RidnRiS1LDZY1xf2m2aDDJR|UxKG:oIECuNFYh|ryP MnrVNVA1OTF2N{[=
KB NIfYeJVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MXe0JIQ> M3nwVGROW09? MU\BcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuETVTSJJdqfGhiSVO1NEBw\iByLkWg{txO NVLOXWlnOTB2MUG0O|Y>
KB M3mwTmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NF7uWGc1KGR? MV\EUXNQ MVfBcpRqeHKxbHnm[ZJifGm4ZTDhZ5Rqfmm2eTDh[4FqdnO2IHTyeYcuemW|aYP0ZY51KEuEVkKwR{B4cXSqIFnDOVAhd2ZiMD60JO69VQ>? MnzvNVA1OTF2N{[=
HTB-54 MVfDfZRwfG:6aXOgRZN{[Xl? M1SxfFExOCEQvF2= M{DXU2lEPTB;MT6xJO69VQ>? Mn;FNVA1PzZ6NkG=
MEL-AC NEDTNJZEgXSxdH;4bYMhSXO|YYm= NF\0SGMyODBizszN NVftRnJ[UUN3ME2xMlIh|ryP MWmxNFQ4Pjh4MR?=
DU145 MlPJR5l1d3SxeHnjJGF{e2G7 MnH6O|IhcA>? MYLJR|UxRTBwMEWyPEDPxE1? M3exO|ExPDd7Mkiy
K562R Mkn1R5l1d3SxeHnjJGF{e2G7 NV7pUINXPzJiaB?= MUPJR|UxRTBwMEG1OkDPxE1? NVLm[pd5OTB2N{myPFI>
PACA-2 NFj4Vm1EgXSxdH;4bYMhSXO|YYm= MVm2JIQ> MVPFSFUxRTBwMEC2N{DPxE1? M{jFSVExPDl6MkG0
3Y1 NITTdFVEgXSxdH;4bYMhSXO|YYm= Ml[yO|IhcA>? MXrHTVUxRTBwMECzPEDPxE1? NXmyXVQxOTB4OUG3NVA>
P388 MXTDfZRwfG:6aXOgRZN{[Xl? NEH5S4E4OiCq NVfP[WNZT0l3ME2wMlAyOiEQvF2= NGLV[YgyODZ7MUexNC=>
HMV-1 NUXrTHBYT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MX7JR|UxRTBwMkig{txO MmH4NVA3QTh2M{[=
MKN-1 NEf1TWFIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NGXmfopKSzVyPUCuOlQh|ryP M{nTTlExPjl6NEO2
PC14 NHztN41Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MkTvTWM2OD1zLkmg{txO MnfiNVA3QTh2M{[=
T24 M{nPeGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3fwc2lEPTB;MD62OEDPxE1? M1jrTFExPjl6NEO2
U937 M1Ltb2Z2dmO2aX;uJGF{e2G7 NUG1V5NFOjRiaB?= Mlr1SG1UVw>? NU\RbJFIUW6qaXLpeJMhcW6lb4Lwc5JifGmxbjDv[kBcO0ifdHj5cYllcW6nIIfpeIghUUN3MDDv[kAxNjFizszN MV:xNFc5ODlzMR?=
H460pv8/eto MXrDfZRwfG:6aXOgRZN{[Xl? NXz5b4VPOTByIN88US=> M17ZRlQ5KGh? M1XxenNidGmwZR?= NWDjN2h5UUN3ME2wMlAzKM7:TR?= NUnLdVdbOTB5OEC5NVM>
H460pv8 MWrDfZRwfG:6aXOgRZN{[Xl? MX6xNFAh|ryP MljuOFghcA>? NXzM[|ZRW2GuaX7l MX7JR|UxRTBwMEC0JO69VQ>? MorhNVA4QDB7MUO=
MDA-231 MVnDfZRwfG:6aXOgRZN{[Xl? NFLLWW0yODBizszN M1j3[VQ5KGh? MX;TZYxqdmV? MnXGR5l1d3SxeHnjbZR6KGGpYXnud5QhVUSDLUKzNUBk\WyuIDjFVk0tTUeIUjuscZV1[W62IIC1N{khf2m2aDDJR|UxKG:oIECuNFA3KM7:TR?= MXKxNFc5ODlzMx?=
MDA-468 M4DETWN6fG:2b4jpZ{BCe3OjeR?= MXOxNFAh|ryP M4f4[|Q5KGh? MYTTZYxqdmV? NGL2WZNEgXSxdH;4bYNqfHliYXfhbY5{fCCPRFGtOFY5KGOnbHygLGVTNSxiYX3wcIlncWWmIFXHSnItKG23dHHueEBxPTNrIIfpeIghUUN3MDDv[kAxNjByNTFOwG0> NX[xXXEyOTB5OEC5NVM>
NCI-H226 MWDDfZRwfG:6aXOgRZN{[Xl? Mm\xNVAxKM7:TR?= M3;ueVQ5KGh? NH7Ib2RU[WyrbnW= NVX1bJQ3S3m2b4TvfIlkcXS7IHHnZYlve3RiTlPJMWgzOjZiY3XscEApdXW2YX70JJA2Oylid3n0bEBKSzVyIH;mJFAvODB6IN88US=> NH3sXoMyODd6MEmxNy=>
NCI-H322 MnK5R5l1d3SxeHnjJGF{e2G7 NVHUbVdROTByIN88US=> NXjU[5VHPDhiaB?= M4CyU3NidGmwZR?= NFrlSoxEgXSxdH;4bYNqfHliYXfhbY5{fCCQQ1mtTFMzOiClZXzsJEhufXSjboSgdFU{MSC5aYToJGlEPTBib3[gNE4xOyEQvF2= NXiyOldYOTB5OEC5NVM>
NCI-H358 NXvGZYVzS3m2b4TvfIlkKEG|c3H5 MX6xNFAh|ryP NGjDdVc1QCCq M1nyZnNidGmwZR?= NGfBOmZEgXSxdH;4bYNqfHliYXfhbY5{fCCQQ1mtTFM2QCClZXzsJEhufXSjboSgdFU{MSC5aYToJGlEPTBib3[gNE4yOyEQvF2= NEHoe|AyODd6MEmxNy=>
NCI-H647 M3[yXmN6fG:2b4jpZ{BCe3OjeR?= MoLnNVAxKM7:TR?= M3nCOlQ5KGh? MnzUV4FtcW6n MWDDfZRwfG:6aXPpeJkh[WejaX7zeEBPS0lvSE[0O{Bk\WyuIDjteZRidnRicEWzLUB4cXSqIFnDOVAhd2ZiMD6wNFEh|ryP NEC0TYoyODd6MEmxNy=>
SKBR-3 NYjJemNKS3m2b4TvfIlkKEG|c3H5 NUnX[GtSOTByIN88US=> NYHwNJk6PDhiaB?= MljLV4FtcW6n NVnxcJVrS3m2b4TvfIlkcXS7IHHnZYlve3RiU1vCVk0{KGOnbHygLGVTNWGvcHzp[olm\CCnclKyMI12fGGwdDDwOVMqKHerdHigTWM2OCCxZjCwMlAzKM7:TR?= NFzaPIkyODd6MEmxNy=>
T47D M{DmfWN6fG:2b4jpZ{BCe3OjeR?= MXyxNFAh|ryP NYPiXpA4PDhiaB?= NX:ydINCW2GuaX7l M4LiV2N6fG:2b4jpZ4l1gSCjZ3HpcpN1KFR2N1SgZ4VtdCBqRWKrMI12fGGwdDDwOVMqKHerdHigTWM2OCCxZjCwMlAxPyEQvF2= NHi4T4MyODd6MEmxNy=>
ZR-75-1 MlvwR5l1d3SxeHnjJGF{e2G7 MXSxNFAh|ryP MYe0PEBp MoL3V4FtcW6n MWTDfZRwfG:6aXPpeJkh[WejaX7zeEBbWi15NT2xJINmdGxiKFXSL{xx\3JtLH31eIFvfCCyNUOpJJdqfGhiSVO1NEBw\iByLkCwPUDPxE1? NX7SUFRLOTB5OEC5NVM>
L1210 NF\WOIFEgXSxdH;4bYMhSXO|YYm= M3;NXWlEPTB;MD6wNlUh|ryP MXixNFg6ODF4Nx?=
D40 NHXhU4lEgXSxdH;4bYMhSXO|YYm= NYLhU29sOjRiaB?= NEfjSJpFVVOR NGraXnhKSzVyPUGuNVczKM7:TR?= NXHHRllGOTB7NU[yNVQ>
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CMK M4Htdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NICz[VBKSzVyPUe3MlQzKG6P MV\TRW5ITVJ?
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CAL-39 M4naU2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M4fTOGlEPTB;OUiuNlQhdk1? M3LRSXNCVkeHUh?=
LS-441N MXjHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXjJR|UxRTl7LkC2JI5O M1;yXHNCVkeHUh?=
G-402 NX33TZRqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NW\UNYpzUUN3ME25PU4xQSCwTR?= MojsV2FPT0WU
MES-SA MmLOS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MlLVTWM2OD17OT61N{BvVQ>? NH;TWW9USU6JRWK=
FADU NVzURmQ6T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= Mkf2TWM2OD1zMEKuNVghdk1? M33pW3NCVkeHUh?=
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KYSE-410 MlP1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1zNUmlEPTB;MUCzMlQ3KG6P MVzTRW5ITVJ?
NCI-H1618 NVG4SHVuT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MVrJR|UxRTFyND6zOUBvVQ>? MUPTRW5ITVJ?
AU565 NHnZWWpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MV\JR|UxRTFyND61NUBvVQ>? NH75bZZUSU6JRWK=
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NCI-H596 M1HRdmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NYPndYpwUUN3ME2xNFgvODFibl2= MV7TRW5ITVJ?
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HCC1569 M{exSGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIDsb5pKSzVyPUGwPU4{PCCwTR?= MnTWV2FPT0WU
NCI-H2170 M2HPWmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2PFT2lEPTB;MUC5MlkzKG6P Mm\NV2FPT0WU
ARH-77 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NYrk[3FJUUN3ME2xNFkvQTlibl2= M1exS3NCVkeHUh?=
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SJSA-1 MkHDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NXjw[o9FUUN3ME2xNVEvODVibl2= MknZV2FPT0WU
SW626 MnK5S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MULJR|UxRTFzMT63NUBvVQ>? Mn7DV2FPT0WU
EHEB M4K4XGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUTQbWJPUUN3ME2xNVIvPTlibl2= NGTKXoJUSU6JRWK=
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MZ7-mel MUPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NILLPGhKSzVyPUGxNk45PCCwTR?= MUTTRW5ITVJ?
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TK10 MV7Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MlfSTWM2OD1zMUSuOkBvVQ>? NILVd4tUSU6JRWK=
SW13 M2jBemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NEHGTFlKSzVyPUGxOU4zPiCwTR?= M1qzcnNCVkeHUh?=
HCC1937 NG\RT3pIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mlq5TWM2OD1zMUWuPFQhdk1? M{W4[XNCVkeHUh?=
SK-NEP-1 NVLyeXdPT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2TCVGlEPTB;MUG2MlI3KG6P NFPhPJJUSU6JRWK=
OVCAR-5 NV3uUVViT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M1XKPGlEPTB;MUG2MlgyKG6P MYTTRW5ITVJ?
SW837 M{T4Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUTobppPUUN3ME2xNVcvQTVibl2= MkHRV2FPT0WU
BFTC-909 NIHXUWJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NYLBRYFOUUN3ME2xNVgvPDhibl2= M2rlZnNCVkeHUh?=
SW872 MkTDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M{XVTWlEPTB;MUKwMlgyKG6P NXrCNJN7W0GQR1XS
SF539 M2PZ[Wdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NH\1c5NKSzVyPUGyNU44OiCwTR?= NFTlfWNUSU6JRWK=
MLMA NGTFUFJIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NF\weJFKSzVyPUGyNk4xQCCwTR?= M3vvNHNCVkeHUh?=
BL-41 MlHuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MXPJR|UxRTF{Mj6yNkBvVQ>? Mln5V2FPT0WU
LB647-SCLC Mn\3S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NUD6THlVUUN3ME2xNlMvOzVibl2= MlXzV2FPT0WU
NCI-H1703 MoDVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MX\JR|UxRTF{Nj60NkBvVQ>? Mki2V2FPT0WU
KURAMOCHI M4XEfWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NXT2PVYzUUN3ME2xNlYvPzZibl2= NETBXGxUSU6JRWK=
ATN-1 MmfBS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NHOyR4JKSzVyPUGyO{44KG6P MlLRV2FPT0WU
SK-CO-1 MnfDS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NFvLW5ZKSzVyPUGyPU4zOSCwTR?= NG\IfHlUSU6JRWK=
RPMI-7951 MojaS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWrJR|UxRTF|MD6xOEBvVQ>? M1XXSXNCVkeHUh?=
NCI-H1648 NULQXYxwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHvUemNKSzVyPUGzNU46PCCwTR?= NV7PVIVbW0GQR1XS
NCI-H64 MXHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MoH4TWM2OD1zM{KuOlUhdk1? M{PBfHNCVkeHUh?=
HT-3 M1TLUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MWHJR|UxRTF|Mz6wOEBvVQ>? M2PEenNCVkeHUh?=
NCI-H1770 MonuS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NVPaR4ROUUN3ME2xN|MvOjFibl2= MknhV2FPT0WU
NB13 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NUD0bJdwUUN3ME2xN|QvPTVibl2= M{O1cXNCVkeHUh?=
NCI-H747 M1\V[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MULJR|UxRTF|NT6wNkBvVQ>? MlrVV2FPT0WU
ML-2 NGiwTnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M2XNVWlEPTB;MUO1MlE3KG6P M1LJUXNCVkeHUh?=
CA46 NWHGU2R1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYrme|dtUUN3ME2xN|YvQDhibl2= MUXTRW5ITVJ?
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JiyoyeP-2003 M{HQdGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX;RS|liUUN3ME2xN|cvQCCwTR?= M{TCPHNCVkeHUh?=
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LU-99A M2PlRWdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{SyNmlEPTB;MUS2MlU2KG6P MYfTRW5ITVJ?
NCI-H748 MnznS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M4rjcmlEPTB;MUS3MlMyKG6P NGH0blVUSU6JRWK=
SK-MEL-3 NIjBNZVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Mk\tTWM2OD1zNEeuOlEhdk1? M2HYenNCVkeHUh?=
SNB75 MnTVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTF2OD62OUBvVQ>? MWXTRW5ITVJ?
BB65-RCC NVnPXJJbT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnHmTWM2OD1zNEmuN|Ehdk1? MX3TRW5ITVJ?
NCI-H1355 NEfwS|lIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NUfYfFRiUUN3ME2xOVIvOjRibl2= NVjCN4hqW0GQR1XS
SCC-25 NXPBdI5KT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NX7VR3dvUUN3ME2xOVMvPzNibl2= Mki1V2FPT0WU
OAW-42 NF;NNHZIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M1:2TWlEPTB;MUWzMlg1KG6P MYfTRW5ITVJ?
MONO-MAC-6 MV\Hdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NFziVZBKSzVyPUG1OE4{PiCwTR?= NFfWeGNUSU6JRWK=
WSU-NHL MmC1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYrPZ2dDUUN3ME2xOVQvPThibl2= NELBSndUSU6JRWK=
SNU-423 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVPDeWFpUUN3ME2xOVUvOzRibl2= M4XjW3NCVkeHUh?=
SBC-1 MmnoS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mn;xTWM2OD1zNU[uOEBvVQ>? NGi2[2tUSU6JRWK=
OVCAR-4 MnTTS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NH3ZTlhKSzVyPUG1Ok44QCCwTR?= MoPpV2FPT0WU
NCI-H2347 MnjVS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M2fCT2lEPTB;MUW4MlM1KG6P MYnTRW5ITVJ?
CAMA-1 MlXyS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NYPZfWlUUUN3ME2xOVkvOzNibl2= M2nY[HNCVkeHUh?=
GDM-1 MUHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MY\JR|UxRTF3OT63JI5O NXK0SJlEW0GQR1XS
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no-10 NFzMWm5Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= Moj1TWM2OD1zNkCuN|ghdk1? MYnTRW5ITVJ?
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Hs-578-T NHPqdpVIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MmnCTWM2OD1{LkCxO|gzKM7:TR?= NF7sN25USU6JRWK=
HCC70 NHfaVHpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MX7JR|UxRTJwMEW0OlMh|ryP MkLXV2FPT0WU
SW1088 MWTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? M1XC[mlEPTB;Mj6wOlk{OSEQvF2= MYHTRW5ITVJ?
NB6 NF\5Oo1Iem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NEXpcXFKSzVyPUKuNVg2KM7:TR?= M1XJXnNCVkeHUh?=
KU-19-19 NHHxXnpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NV\YZoRLUUN3ME2yMlI3QTF5IN88US=> NWHud25OW0GQR1XS
A704 MWHHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NXPG[5BMUUN3ME2yMlI6OTN3IN88US=> NFznc5lUSU6JRWK=
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HCT-15 NUTMXmN4T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWSyN25IUUN3ME2yMlM2PDZ4IN88US=> NGfGNFhUSU6JRWK=
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GMS-10 MnnMS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NEXQWW9KSzVyPUKuN|k4OzhizszN NEG2RnFUSU6JRWK=
TGBC24TKB Mnm1S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV\JR|UxRTJwNEG0Nlkh|ryP MXPTRW5ITVJ?
C2BBe1 MXfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmLBTWM2OD1{LkWxN|I5KM7:TR?= M{nNVnNCVkeHUh?=
SK-N-DZ MnXJS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MWDJR|UxRTJwNU[1PVIh|ryP M2LoWHNCVkeHUh?=
SK-N-AS MXzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NX3pOINKUUN3ME2yMlc{ODF3IN88US=> NYn1RXp1W0GQR1XS
T98G NVTvTmRnT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NYHxRlVVUUN3ME2yMlgxPDBzIN88US=> NEOyfGdUSU6JRWK=
DMS-79 Mk\wS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? NIHBRZRKSzVyPUKuPVA6QDhizszN MkLYV2FPT0WU
SNU-475 NIqze5NIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= MYTJR|UxRTJwOUK1O|Ih|ryP NH3rbItUSU6JRWK=
COLO-792 M3flemdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MkfyTWM2OD1|LkCyPFch|ryP MV\TRW5ITVJ?
YAPC M{S1[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGXBfI1KSzVyPUOuN|I5OzJizszN NYj5fm11W0GQR1XS
UACC-812 M1zCOGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M3LydGlEPTB;Mz6zN|c{QCEQvF2= NGfqZmRUSU6JRWK=
Mewo MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NEn3NI5KSzVyPUOuOFYxQDZizszN NFO1NoNUSU6JRWK=
COLO-824 M3jofGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NX\QSlFnUUN3ME2zMlU4Ozd6IN88US=> NX31e5lWW0GQR1XS
SCC-9 NWXWToFzT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MkP4TWM2OD1|LkmxN|A6KM7:TR?= MmL6V2FPT0WU
CP50-MEL-B NUHm[FVHT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M{XGd2lEPTB;Mz65PFcxOyEQvF2= NXrnO|l{W0GQR1XS
NCI-H345 NUT4c|RFT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHXZVG1KSzVyPUSuNFEyPzRizszN M4\oZnNCVkeHUh?=
COR-L88 MVzHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MUDJR|UxRTRwM{m1PEDPxE1? NEXHVG5USU6JRWK=
RCM-1 MlmxS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MV3JR|UxRTRwNEmyO|Ih|ryP M3LvPHNCVkeHUh?=
JVM-3 M1S3Omdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{HmRWlEPTB;ND62NFYyQCEQvF2= NVrySWtPW0GQR1XS
ECC4 MVvHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NVL6TZk3UUN3ME20Mlc3PTRzIN88US=> MXPTRW5ITVJ?
MDA-MB-453 MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? Ml;kTWM2OD12Lki1NVM1KM7:TR?= NEHE[WVUSU6JRWK=
SK-N-FI MVPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NHrpbHNKSzVyPUWuNlI5PzdizszN M3TY[XNCVkeHUh?=
D-502MG Mn;MS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MknaTWM2OD13LkK0NlYzKM7:TR?= MWnTRW5ITVJ?
NCI-H2227 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NH\pWHZKSzVyPUWuN|YzOzFizszN M13XcnNCVkeHUh?=
DMS-53 NI\HOGdIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NWHT[I84UUN3ME22MlE4OTB4IN88US=> M1G5R3NCVkeHUh?=
LB771-HNC MYTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXXJR|UxRTZwNE[4NFYh|ryP MWrTRW5ITVJ?
BB49-HNC Mkj0S5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? Mki3TWM2OD15LkSxO|kyKM7:TR?= NGGxNG9USU6JRWK=
MDA-MB-415 NH7lOmpIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= NXvXTlAzUUN3ME23MlU4OzB|IN88US=> Mnr4V2FPT0WU
TALL-1 MWPHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MXTJR|UxRTdwOU[4PEDPxE1? NFjadVRUSU6JRWK=
HCC1395 NWPJPIZ1T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NHG1W5hKSzVyPUiuNVUyOjJizszN NGjVU3ZUSU6JRWK=
EFE-184 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MYrJR|UxRThwM{eyNVYh|ryP M3S0bnNCVkeHUh?=
EC-GI-10 MUTHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? MmHtTWM2OD16Lk[4Olg3KM7:TR?= M3zpVHNCVkeHUh?=
TT NV\TXYpqT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NH;N[W5KSzVyPUmuNFY6PjZizszN Mn70V2FPT0WU
U-2-OS NVrC[lN2T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= NWLUOopMUUN3ME25MlgyOzl|IN88US=> NI[0enhUSU6JRWK=
SHP-77 M4PJ[mdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NIrPRYJKSzVyPUmuPVAzQSEQvF2= MnrXV2FPT0WU
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A498 M2rWVmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M2j4OmlEPTB;MUiuNlk4PiEQvF2= NFfzbZhUSU6JRWK=
LS-513 M4\HN2dzd3e2aDDJcohq[mm2aX;uJGF{e2G7 MlyxTWM2OD1zOD62NFk{KM7:TR?= MlzSV2FPT0WU
K052 MVnHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NGXHcXBKSzVyPUKxMlQ1OThizszN M2rhU3NCVkeHUh?=
SiHa NWPOPZE5T3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= M2X4OGlEPTB;MkOuN|M4OSEQvF2= M3\ibnNCVkeHUh?=
RCC10RGB M3rOUmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NGHsfGFKSzVyPUOwMlA3OTFizszN NUH0SYJHW0GQR1XS
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C32 Mo\pS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? M1TSe2lEPTB;M{KuPFczKM7:TR?= NInyTJdUSU6JRWK=
SW684 NW\wd2FwT3Kxd4ToJGlvcGmkaYTpc44hSXO|YYm= MnPCTWM2OD1|ND6xOVYyKM7:TR?= M3LBS3NCVkeHUh?=
SK-MEL-28 M{fQXmdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 M{Sz[WlEPTB;M{WuOVM4PyEQvF2= NIrSWnZUSU6JRWK=
SK-MEL-1 NI\JZoxIem:5dHigTY5pcWKrdHnvckBCe3OjeR?= M4S2OWlEPTB;M{[uO|U1PyEQvF2= NH\qPFZUSU6JRWK=
NCI-H720 MljHS5Jwf3SqIFnubIljcXSrb36gRZN{[Xl? MVvJR|UxRTRzLkm3NlYh|ryP MXnTRW5ITVJ?
P31-FUJ MUfHdo94fGhiSX7obYJqfGmxbjDBd5NigQ>? NWCwU4w6UUN3ME20OE4{Ojh2IN88US=> NUXjd29WW0GQR1XS
NCI-H524 M176fGdzd3e2aDDJcohq[mm2aX;uJGF{e2G7 NUfVW2d4UUN3ME20PE45OjR6IN88US=> NFXRWmFUSU6JRWK=

... Click to View More Cell Line Experimental Data

Assay
Methods Test Index PMID
Western blot
POU5F1 / SOX2 / NANOG; 

PubMed: 27929731     


NT2/D1 cells treated with 10 nM of doxorubicin were subjected to WB analysis for pluripotency factors (POU5F1, NANOG, SOX2) and differentiation markers (TUBB3).

ATG5 / ATG7 / SQSTM1 / LC3A-II / LC3B-II / p-mTOR; 

PubMed: 27929731     


NT2/D1 cells treated with 10 nM of doxorubicin were subjected to WB analysis for autophagy markers (ATG5, ATG7, SQSTM1, LC3A-II, LC3B-II and p-MTOR). 

P62 / LC3II; 

PubMed: 28208617     


(A,B) Doxorubicin induces autophagy in pancreatic cancer cells. Western blot analysis of LC3-II and p62 protein levels in Mia PaCa-2 and Panc-1 cell lines treated with indicated concentrations of doxorubicin for 24 h, or treated with 2.5 μM doxorubicin for indicated times.

p-AKT(T308) / p-Akt(S473) / Akt / p-PRAS40(T246) / PRAS40 / p-H2A.X(S139); 

PubMed: 26027929     


MCF10A cells were serum-starved and treated with (A) 2 μM doxorubicin over a 10 hour time course.

27929731 28208617 26027929
Growth inhibition assay
Cell viability; 

PubMed: 25871400     


Salinomycin enhances the cytotoxicity of doxorubicin in HCC cellsRelative cell viability (mean ± SD) for salinomycin (black), doxorubicin (blue) and doxorubicin plus salinomycin (red) in HuH-7, HepG2, SNU-449 and SNU-387 cells.

25871400
Immunofluorescence
LC3; 

PubMed: 24970805     


Doxorubicin induces GFP-LC3 puncta formation in hepatocellular carcinoma cells. SK-Hep1 cells were treated with doxorubicin (2.5 μM) or CQ (10 μM) plus doxorubicin for 7 h and cells were examined with a confocal microscope for GFP-LC3 punctuation/aggregation.

24970805
ELISA
OPN; 

PubMed: 24963635     


A549 were exposed to 50 nM doxorubicin or PBS for 48 h. OPN concentrations in cell culture media were determined by ELISA.

24963635
In vivo In vivo, Doxorubicin in combination with adenoviral MnSOD (AdMnSOD) plus 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU) has the greatest effect in decreasing the volumes of MB231 tumors and prolonging survival of mice. [1] Although its use is limited by the chronic and acute toxic side effects it produces, Doxorubicin is essential in treating breast and oesophageal carcinomas, solid tumours in childhood, osteosarcomas, Kaposi's sarcoma, soft tissue sarcomas, and Hodgkin and non-Hodgkin lymphomas. [2]

Protocol

Animal Research:[1]
- Collapse
  • Animal Models: Female athymic nude mice injected s.c. with MB231 cells
  • Dosages: 3 mg/kg/day
  • Administration: Delivered intratumorly
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL warmed (172.41 mM)
Water 20 mg/mL (34.48 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 579.98
Formula

C27H29NO11.HCl

CAS No. 25316-40-9
Storage powder
in solvent
Synonyms NSC 123127, DOX
Smiles CC1C(C(CC(O1)OC2CC(CC3=C2C(=C4C(=C3O)C(=O)C5=C(C4=O)C(=CC=C5)OC)O)(C(=O)CO)O)N)O.Cl

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
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Step 2: Enter the in vivo formulation ()
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Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
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*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and MSDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
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* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and MSDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
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Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04423913 Not yet recruiting Other: Administration of embolization beads Prostate Cancer Centre Hospitalier Universitaire de Nīmes December 1 2020 Phase 2
NCT04204941 Recruiting Drug: Tazemetostat|Drug: Placebo|Drug: Doxorubicin HCl Advanced Soft Tissue Sarcoma|Advanced Epithelioid Sarcoma Epizyme Inc. December 19 2019 Phase 3
NCT04032964 Not yet recruiting Drug: L19TNF|Drug: DOXORUBICIN Soft Tissue Sarcoma Philogen S.p.A. September 2019 Phase 1
NCT03975205 Not yet recruiting Drug: Doxorubicin Lymphoma Leukemia Sutphin Drugs|Bharath Charitable Cancer Hospital and Institute July 1 2019 Early Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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Frequently Asked Questions

  • Question 1:

    Why the IC50 of the compound varied over time in our measurement?

  • Answer:

    Sometimes the IC50 value might vary between different batch of compounds due to the slight differences in purity. If different IC50 was seen in the same batch of compound, the problem may lies in the process of dissolvement. Make sure that you see no particles and the solution is clear with your stock solution.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID