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5-Hydroxymethylcytosine

Name: 5-Hydroxymethylcytosine
SKU: E0027
Availability: InStock
Rating: 5 (1 reviews)

Cat.No.E0027

5-Hydroxymethylcytosine (5hmC) is a naturally existing component in mammalian genomic DNA and is regarded as the sixth DNA base. This compound is an intermediate metabolite in active DNA demethylation. It is an epigenetic mark known to affect global gene expression in mammals.

Chemical Structure

Molecular Weight: 141.13

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: E002701 DMSO]200 mg/mL]true]Water]Insoluble]false]Ethanol]Insoluble]false Purity: 99.89%

99.89

Related Targets	Epigenetic Reader Domain HDAC JAK Histone Methyltransferase AMPK PKC PARP HIF Histone Acetyltransferase Aurora Kinase Sirtuin PRMT Histone Demethylase EZH1/2 DNA Methyltransferase Pim LSD1 MLL JMJD G9a/GLP NSD FTO MicroRNA PAD SETD SMYD DOT1 COMT WDR5 HNMT
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Chemical Information, Storage & Stability

Molecular Weight	141.13	Formula	C₅H₇N₃O₂	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	1123-95-1	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	5hmC			Smiles	NC1=C(CO)C=NC(=O)N1

Solubility

In vitro
Batch:

DMSO : 200 mg/mL (1417.13 mM) Warmed with 50°C water bath; Ultrasonicated;
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	10.000mg/ml (70.86mM)	Taking the 1 mL working solution as an example, add 50 μL of 200 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

References	[1] https://pubmed.ncbi.nlm.nih.gov/27001475/ [2] https://pubmed.ncbi.nlm.nih.gov/32385352/ [3] https://pubmed.ncbi.nlm.nih.gov/28925386/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04741945	Recruiting	Preleukemia\|Myelodysplastic Neoplasm\|Cytopenia\|Preleukemic Anemia	Kirsten Grønbæk\|Steno Diabetes Center Copenhagen\|Zealand University Hospital\|Van Andel Research Institute\|Herlev Hospital\|Technical University of Denmark\|Region Hovedstadens Apotek\|University of Copenhagen\|Rigshospitalet Denmark	December 13 2021	Phase 2
NCT05318989	Completed	Genitourinary Agents	Żelazna Medical Centre LLC\|Laboratory of Genetics and Human Genomics University of Gdańsk	February 8 2021	--
NCT03919539	Unknown status	Drug Resistance to Famitinib and Camrelizumab\|Biomarkers for Efficacy and Toxicity	Peking University People''s Hospital\|Peking University\|Northwestern University	December 1 2019	--
NCT03999723	Recruiting	Myelodysplastic Syndromes\|Acute Myeloid Leukemia\|Chronic Myelomonocytic Leukemia	Kirsten Grønbæk\|Van Andel Institute - Stand Up To Cancer Epigenetics Dream Team\|Karolinska University Hospital\|Skane University Hospital\|Sahlgrenska University Hospital Sweden\|University of Southern California\|Imperial College London\|University of Copenhagen\|Zealand University Hospital\|Aalborg University Hospital\|Odense University Hospital\|Technical University of Denmark\|Aarhus University Hospital\|Uppsala University Hospital\|Rigshospitalet Denmark	September 11 2019	Phase 2
NCT03526666	Completed	Myelodysplastic Syndromes\|Acute Myeloid Leukemia\|Chronic Myelomonocytic Leukemia\|MDS\|AML\|CMML	Van Andel Research Institute	November 1 2017	--

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Handling Instructions

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