CAS No. 1123-95-1
Batch:
Purity:99.89
Chemical Structure
Check the
5-Hydroxymethylcytosine
product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.
| IUPAC Name | 6-amino-5-(hydroxymethyl)-1H-pyrimidin-2-one | |
|---|---|---|
| CAS Number | 1123-95-1 | |
| Molecular Formula |
C5H7N3O2 |
|
| Molecular Weight (MW) | 141.13 | |
| SMILES | NC1=C(CO)C=NC(=O)N1 | |
| InChIKey | RYVNIFSIEDRLSJ-UHFFFAOYSA-N |
5-Hydroxymethylcytosine is a modified pyrimidine nucleobase and a key epigenetic mark in mammalian genomic DNA, often referred to as the sixth DNA base. It serves as an intermediate metabolite generated during active DNA demethylation catalyzed by ten-eleven translocation (TET) family dioxygenases. By modulating chromatin structure and transcription factor recruitment, 5-hydroxymethylcytosine plays crucial roles in regulating global gene expression, cellular differentiation, and epigenetic reprogramming. [1]
|
Concentration
Volume
Mass
|
|---|
Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
| Solvent | Solubility & Note |
|---|---|
| DMSO |
200 mg/mL
(1417.13 mM)
; Warmed with 60°C water bath
; Ultrasonicated
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.) |
| Water | Insoluble |
| Ethanol | Insoluble |
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
- Preheat media/saline to 37°C.
- Mix rapidly (pipette/vortex) upon addition.
- For complex mixes, always add the aqueous phase last.
- Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).
- 5-Hydroxymethylcytosine Clinical Trials
- 5-Hydroxymethylcytosine Solubility in DMSO
- 5-Hydroxymethylcytosine Stock Solution
- 5-Hydroxymethylcytosine Storage
- 5-Hydroxymethylcytosine Stability
- 5-Hydroxymethylcytosine Molecular Weight
- 5-Hydroxymethylcytosine SMILES
- 5-Hydroxymethylcytosine Chemical Structure (2D and 3D)
- 5-Hydroxymethylcytosine SDS｜MSDS
Note: Technical data last updated: Sep 1, 2026.