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NOS Inhibitors

Cat.No. Product Name Information Product Use Citations Product Validations
S2877 L-NAME HCl L-NAME HCl (NG-Nitroarginine methyl ester, N-Nitro-L-arginine methylester) is a nonselective inhibitor of nitric oxide synthetases (NOS) for nNOS (bovine), eNOS (human), and iNOS (murine), with Ki of 15 nM, 39 nM and 4.4 μM, respectively. This compound can be used to induce animal models of Hypertension.
PLoS Pathog, 2025, 21(4):e1012561
Commun Biol, 2025, 8(1):201
Nat Neurosci, 2024, 10.1038/s41593-024-01604-8
Verified customer review of L-NAME HCl
S8337 1400W 2HCl 1400W (N-(3-(Aminomethyl)benzyl)acetamidine) is a slow, tight binding, and highly selective inhibitor of inducible nitric-oxide synthase(iNOS).
Nat Commun, 2025, 16(1):3570
Biomolecules, 2025, 15(1)125
Nat Commun, 2024, 15(1):2698
S4548 Aminoguanidine hydrochloride Aminoguanidine (Pimagedine, Guanyl hydrazine, Hydrazinecarboximidamide, Imino semicarbazide, Monoaminoguanidine) is a diamine oxidase and nitric oxide synthase inhibitor. It acts to reduce levels of advanced glycation end products (AGEs) through interacting with 3-deoxyglucosone.
Anim Biotechnol, 2021, 1-10
Anim Biotechnol, 2021, 1-8
Am J Cancer Res, 2020, 10(5):1400-1415
S3292 (3R,8S)-Falcarindiol Falcarindiol (FAD, (3R,8S)-Falcarindiol, FaDOH) is a natural polyacetylene compound found rich in many plants of the Umbelliferae family. Falcarindiol suppresses LPS-stimulated expression of inducible nitric oxide synthase (iNOS), tumor necrosis factor alpha (TNFα), interleukin-6 (IL-6), and interleukin-1 beta (IL-1β). Falcarindiol attenuates the LPS-induced activation of JNK, ERK, STAT1, and STAT3 signaling molecules.
Antioxidants (Basel), 2024, 13(12)1533
Front Cell Infect Microbiol, 2023, 13:1128000
Front Pharmacol, 2021, 12:656697
S7920 L-NMMA acetate L-NMMA acetate (Tilarginine Acetate, Methylarginine acetate, NG-Monomethyl-L-arginine acetate, L-NG-monomethyl Arginine acetate) is an acetate salt form of L-NMMA. L-NMMA is a nitric oxide synthase(NOS) inhibitor with an IC50 of 4.1 μM for nNOS.
Stem Cell Res Ther, 2024, 15(1):366
Spectrochim Acta A Mol Biomol Spectrosc, 2022, 277:121280
S9374 2',5'-Dihydroxyacetophenone 2',5'-Dihydroxyacetophenone (DHAP, 2-Acetylhydroquinone, Quinacetophenone) is a mixture of dihydroxyacetophenone isomers is used in food flavouring. This compound significantly inhibits NO production via the suppression of iNOS expression. It significantly decreases levels of the pro-inflammatory cytokines TNF-α and IL-6 by blocking the ERK1/2 and NF-κB signaling pathways.
Pathol Oncol Res, 2019, 25(1):301-309
S5671 Amcinonide Amcinonide (Cyclocort, CL-34699) is an inhibitor of nitric oxide (NO) release from activated microglia with IC50 of 3.38 nM.
E0496 ZLc002 ZLc-002 is a selective inhibitor of nNOS-Capon coupling. ZLc-002 suppresses inflammatory nociception and chemotherapy-induced neuropathic pain.
E5776 Asymmetric dimethylarginine Asymmetric dimethylarginine is an endogenous inhibitor of nitric oxide synthase (NOS). It also functions as a marker of endothelial dysfunction in several pathological states.
S9029 Prim-o-glucosylcimifugin Prim-O-glucosylcimifugin (Cimifugin beta-D-glucopyranoside, Cimifugin 7-glucoside) is a major constituent in Radix Saposhnikovia that has been long used for the treatment of pyrexia, rheumatism, and cancer in traditional Chinese medicine. It shows potential anticancer activity. Prim-O-glucosylcimifugin downregulates the mRNA and protein expression inducible NO synthase (iNOS) and cyclooxygenase 2 (COX-2) in LPS-activated RAW 264.7 macrophages in a concentration-dependent manner.
E4037 L-Canavanine sulfate L-Canavanine sulfate is a selective inhibitor of inducible NO synthase. This compound exhibits IC50 values ranging from 5 to 10 mM in arginine-rich media and causes a limited degree of cytotoxicity in HeLa, Hep G2, and SK-HEP-1 cells.
S3899 Hederagenin Hederagenin (Caulosapogenin, Hederagenol, Hederagenic acid, Astrantiagenin E) is a highly water insoluble triterpenoid compound that can be found in various plants including Hedera helix and Chenopodium quinoa. It exhibits a variety of biological activities, including potent antitumor properties both in vitro and in vivo. This compound inhibits LPS-stimulated expression of iNOS, COX-2, and NF-κB.
S3304 Stylopine Stylopine ((R,S)-Stylopine, Tetrahydrocoptisine) reduces nitric oxide (NO), prostaglandin E2 (PGE2), tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta), and the IL-6 production and cyclooxygenase-2 (COX-2) activity caused by the LPS stimulation. This compound is a major component of the leaf of Chelidonium majus L.
S5542 7-Nitroindazole 7-Nitroindazole, a heterocyclic compound, acts as a selective inhibitor for neuronal nitric oxide synthase showing a 10-fold selectivity for neuronal NOS.
S3931 Ginsenoside Rd Ginsenoside Rd (Panaxoside Rd, Sanchinoside Rd), a minor ginseng saponin, has several pharmacological activities such as immunosuppressive activity, anti-inflammatory activity, immunological adjuvant, anti-cancer activity and wound-healing activity. This compound inhibits TNFα-induced NF-κB transcriptional activity with an IC50 of 12.05±0.82 μM in HepG2 cells. It inhibits expression of COX-2 and iNOS mRNA. This chemical also inhibits Ca2+ influx. It inhibits CYP2D6, CYP1A2, CYP3A4, and CYP2C9, with IC50s of 58.0±4.5 μM, 78.4±5.3 μM, 81.7±2.6 μM, and 85.1±9.1 μM, respectively.
Drug Des Devel Ther, 2022, 16:2767-2782
J Ginseng Res, 2022, 46(5):700-709
J Ethnopharmacol, 2021, S0378-8741(21)00169-0
S3924 Ginsenoside Rb1 Ginsenoside Rb1 (Gypenoside Ⅲ) is a protopanaxadiol that has diverse in vitro and in vivo effects, including neuroprotective, anti-inflammatory, and anti-obesity actions. This compound, a main constituent of the root of Panax ginseng, inhibits Na+, K+-ATPase activity with IC50 of 6.3±1.0 μM. It also inhibits IRAK-1 activation and phosphorylation of NF-κB p65. This chemical reduces the expressions of TLR3, TLR4 and TRAF-6, and down-regulates the levels of TNF-α, IFN-β and iNOS.
J Biol Chem, 2024, 300(8):107542
J Ethnopharmacol, 2021, S0378-8741(21)00169-0
J Biol Chem, 2021, S0021-9258(21)00790-0
S9208 Ginsenoside Rb3 Ginsenoside Rb3, extracted from the plant Panax ginseng, plays important roles in cardiovascular diseases, including myocardial ischemia-reperfusion (I/R) injury. This compound (0.1-10 μM) is tested for inhibition of tumor necrosis factor-α (TNF)-induced nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) luciferase reporter activity using a human kidney 293T cell-based assay. It shows the significant activity with an IC50 of 8.2 μM. This chemical also inhibits the induction of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) messenger Ribonucleic acid (mRNA) in a dose-dependent manner after HepG2 cells have been treated with TNF-α (10 ng/mL).
Chin Med, 2024, 19(1):70
S5640 Ethyl caffeate Ethyl caffeate, a naturally occurring compound found in Bidens pilosa, suppresses NF-kappaB activation and its downstream inflammatory mediators, iNOS, COX-2 and PGE2 in vitro.
Int J Mol Sci, 2023, 24(12)9997
S6850 NE 52-QQ57 NE 52-QQ57 is a selective, and orally available antagonist of G-protein coupled receptor 4 (GPR4) with IC50 of 0.07 μM. This compound effectively blocks GPR4-mediated cAMP accumulation with IC50 of 26.8 nM in HEK293 cells. The antagonism of GPR4 with this chemical significantly inhibits the AGE-induced increased expression of several key inflammatory cytokines and signaling molecules, including tumor necrosis factor-α (TNF-α), interleukin (IL)-1β, IL-6, inducible nitric oxide synthase (iNOS), nitric oxide (NO), cyclooxygenase 2 (COX2), and prostaglandin E2 (PGE2).
Exp Hematol Oncol, 2024, 13(1):13
S9502 Madecassic acid Madecassic acid (Brahmic acid), a natural triterpene first isolated from C. asiatica, has diverse anti-inflammatory and anti-diabetic effects, blocking NF-κB activation in macrophages and causing by iNOS, COX-2, TNF-alpha, IL-1beta and IL-6 inhibition.
Drug Des Devel Ther, 2022, 16:3793-3804
S3261 Myrislignan Myrislignan, a lignan isolated from Myristica fragrans Houtt, possesses anti-inflammatory activities. This compound inhibits interleukin-6 (IL-6) and tumour necrosis factor-α (TNF-α). It significantly inhibits the expressions of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2) dose-dependently in LPS-stimulated macrophage cells. It also inhibits the NF-κB signalling pathway activation.
Oxid Med Cell Longev, 2023, 2023:7098313
S0924 Pteryxin Pteryxin ((+)-Pteryxin), a dihydropyranocoumarin derivative found in Apiaceae family, is a potent inhibitor of butyrylcholinesterase (BChE) with IC50 of 12.96 μg/ml. This compound inhibits LPS-induced nitric oxide production in mouse peritoneal macrophages with IC50 of 20 µM. It is potential for Alzheimer's disease (AD) treatment.
E4805 Methylene Blue trihydrate Methylene blue trihydrate (C.I. Basic Blue 9 trihydrate) acts as an inhibitor of soluble guanylyl cyclase (sGC), monoamine oxidase A (MAO-A) and NO synthase (NOS). Methylene blue trihydrate exhibits antimalarial, antidepressant, and anxiolytic activity effects. Methylene Blue trihydrate has potential applications in treating methemoglobinemia, neurodegenerative disorders, and ifosfamide-induced encephalopathy.