Home GPCR & G Protein cAMP inhibitor PACAP 1-27 For research use only.

PACAP 1-27

Pituitary adenylate cyclase activating polypeptide (PACAP 1-27) is a potent PACAP receptor antagonist.

PACAP 1-27 Chemical Structure

PACAP 1-27 Chemical Structure

CAS: 127317-03-7

Purity & Quality Control

Batch: Purity: 99.52%
99.52

PACAP 1-27 Related Products

Signaling Pathway

cAMP Signaling Pathways

cAMP Signaling Pathway

Choose Selective cAMP Inhibitors

Biological Activity

Description Pituitary adenylate cyclase activating polypeptide (PACAP 1-27) is a potent PACAP receptor antagonist.
Targets
PACAP receptor [1]
In vitro
In vitro PACAP27(100 nM) inhibits all Tat-mediated toxic effects including DNA DSBs, and it prevents the induction of neuronal loss induced by Tat(transactivator of transcription). PACAP27, through CCL5, mitigates the effects of Tat-induced neuronal dysfunction exerting its neuroprotective effect[4]. PACAP-27 (EC50=10nM) stimulates glibenclamide-sensitive and DIDS-insensitive iodide efflux in Calu-3 cells[5].
Cell Research Cell lines The pulmonary carcinoma cell line CALU-6
Concentrations 10 pmol/L to 1 μmol/L
Incubation Time 20 h
Method Cells are incubated with PACAP (1-27) (10 pmol/L to 1 μmol/L) or forskolin (5 μmol/L) for 20 h. Thereafter, cells are harvested, and isolation of total RNA, reverse transcriptase-PCR, and subsequent determination of renin mRNA and β-actin mRNA expression using real-time PCR.
In Vivo
In vivo PACAP-27 is potent VIP-like vasodilators of the femoral arterial bed in dogs. In the cardiovascular system, PACAP27 has vasodepressor actions similar to VIP in anesthetized rats. Intra-arterial infusion of PACAP27 induces dose-related increases in peak femoral arterial flow[3].
Animal Research Animal Models beagle dogs
Dosages 0.25, 0.5, 1, 2.5, 5, 10, and 25 pmol/kg
Administration Intra-arterial infusion

Chemical Information & Solubility

Molecular Weight 3147.61 Formula

C142H224N40O39S
CAS No. 127317-03-7 SDF Download PACAP 1-27 SDF
Smiles CCC(C)C(C(=O)NC(CC1=CC=CC=C1)C(=O)NC(C(C)O)C(=O)NC(CC(=O)O)C(=O)NC(CO)C(=O)NC(CC2=CC=C(C=C2)O)C(=O)NC(CO)C(=O)NC(CCCNC(=N)N)C(=O)NC(CC3=CC=C(C=C3)O)C(=O)NC(CCCNC(=N)N)C(=O)NC(CCCCN)C(=O)NC(CCC(=O)N)C(=O)NC(CCSC)C(=O)NC(C)C(=O)NC(C(C)C)C(=O)NC(CCCCN)C(=O)NC(CCCCN)C(=O)NC(CC4=CC=C(C=C4)O)C(=O)NC(CC(C)C)C(=O)NC(C)C(=O)NC(C)C(=O)NC(C(C)C)C(=O)NC(CC(C)C)C(=O)N)NC(=O)CNC(=O)C(CC(=O)O)NC(=O)C(CO)NC(=O)C(CC5=CNC=N5)N
Storage (From the date of receipt)

In vitro
Batch:

Water : 100 mg/mL

DMSO : Insoluble ( Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : Insoluble


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In vivo
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