Home GPCR & G Protein cAMP inhibitor PACAP 6-38 acetate For research use only.

PACAP 6-38 acetate

PACAP 6-38 acetate is a PACAP (pituitary adenylate cyclase-activating polypeptide) non-stimulating competitive antagonist with an IC50 value of 2 nM. It also acts as a functional CARTp antagonist in vivo.

PACAP 6-38 acetate Chemical Structure

PACAP 6-38 acetate Chemical Structure

CAS: 143748-18-9(free base)

Selleck's PACAP 6-38 acetate has been cited by 1 publication

Purity & Quality Control

Batch: Purity: 99.63%
99.63

PACAP 6-38 acetate Related Products

Signaling Pathway

cAMP Signaling Pathways

cAMP Signaling Pathway

Choose Selective cAMP Inhibitors

Biological Activity

Description PACAP 6-38 acetate is a PACAP (pituitary adenylate cyclase-activating polypeptide) non-stimulating competitive antagonist with an IC50 value of 2 nM. It also acts as a functional CARTp antagonist in vivo.
Targets
CARTp [2] PACAP receptor [1]
2 nM
In vitro
In vitro

PACAP 6-38 blocks the CARTp-induced phosphorylation of ERK in differentiated cells[2].
Cell Research Cell lines SK-N-MC neuroblastoma cells
Concentrations 30 μM
Incubation Time 4 h
Method

Cells are incubated for 4 h with 300 nM PACAP-38 in the absence or presence of the PACAP antagonist PACAP (6-38), medium is collected, and proteins are separated by SDS/PAGE and blotted onto PVDF membranes. For APPsα detection, membranes are probed with the antibody 6E10 followed by an anti-mouse antibody labeled with HRP, detection is performed with the ECL plus system.
In Vivo
In vivo

Intravesical administration of the PAC1 receptor antagonist, PACAP(6-38) (300 nM), significantly (p ≤ 0.01) increases intercontraction interval (2.0-fold) and void volume (2.5-fold) in NGF-OE mice. PACAP(6-38) has no effects on bladder function in WT mice. Intravesical administration of PACAP(6–38) (300 nM) significantly (p ≤ 0.01) reduces pelvic sensitivity in NGF-OE mice but is without effect in WT mice[4].
Animal Research Animal Models Male Sprague-Dawley rats
Dosages 0.3 nmol, 0.6 nmol, 3 nmol (1.5 Μl/340g)
Administration i.c.v

Chemical Information & Solubility

Molecular Weight 4083.74 Formula

C182H300N56O45S
CAS No. 143748-18-9(free base) SDF Download PACAP 6-38 acetate SDF
Smiles CSCCC(NC(=O)C(CCC(N)=O)NC(=O)C(CCCCN)NC(=O)C(CCCNC(N)=N)NC(=O)C(CC1=CC=C(O)C=C1)NC(=O)C(CCCNC(N)=N)NC(=O)C(CO)NC(=O)C(CC2=CC=C(O)C=C2)NC(=O)C(CO)NC(=O)C(CC(O)=O)NC(=O)C(NC(=O)C(N)CC3=CC=CC=C3)C(C)O)C(=O)NC(C)C(=O)NC(C(C)C)C(=O)NC(CCCCN)C(=O)NC(CCCCN)C(=O)NC(CC4=CC=C(O)C=C4)C(=O)NC(CC(C)C)C(=O)NC(C)C(=O)NC(C)C(=O)NC(C(C)C)C(=O)NC(CC(C)C)C(=O)NCC(=O)NC(CCCCN)C(=O)NC(CCCNC(N)=N)C(=O)NC(CC5=CC=C(O)C=C5)C(=O)NC(CCCCN)C(=O)NC(CCC(N)=O)C(=O)NC(CCCNC(N)=N)C(=O)NC(C(C)C)C(=O)NC(CCCCN)C(=O)NC(CC(N)=O)C(=O)NC(CCCCN)C(N)=O
Storage (From the date of receipt)

In vitro
Batch:

Water : 100 mg/mL


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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