Home Metabolism Other chemical Cholic acid For research use only.

Cholic acid

Cholic acid is a naturally occurring bile acid that is insoluble in water and is used to treat patients with genetic deficiencies in the synthesis of bile acids.

Cholic acid Chemical Structure

Cholic acid Chemical Structure

CAS: 81-25-4

Selleck's Cholic acid has been cited by 1 publication

Purity & Quality Control

Batch: S374201 DMSO] 81 mg/mL] false] Ethanol] 28 mg/mL] false] Water] Insoluble] false Purity: 98.58%
98.58

Cholic acid Related Products

Biological Activity

Description Cholic acid is a naturally occurring bile acid that is insoluble in water and is used to treat patients with genetic deficiencies in the synthesis of bile acids.
In vitro
In vitro Fosfomycin is known to inhibit MurA by covalently binding to a highly conserved cysteine in the active site of the enzyme. Fosfomycin is a PEP mimetic that acts by alkylating the highly conserved Cys115 (Escherichia coli numbering) of MurA[1]. Fosfomycin exerts immunomodulatory effects by altering lymphocyte, monocyte and neutrophil function. It affects the acute inflammatory cytokine response in vitro and in vivo. It suppresses production of tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β), and IL-1α and increases production of IL-10. Fosfomycin suppresses IL-2 production from T cells, the production of leukotriene B4 (LTB4) from neutrophils, and the expression of IL-8 mRNA by LTB4 from monocytes. Fosfomycin also exhibits an immunomodulatory effect on B-cell activation. Fosfomycin enhances neutrophil phagocytic killing of invading pathogens, even in patients on chronic hemodialysis and renal transplantation. Fosfomycin resulted in enhanced bactericidal ability of neutrophils compared to other antimicrobials. Fosfomycin has the ability to penetrate into biofilms. fosfomycin is considerably active against both Gram-negative and Gram-positive pathogens[2].
In Vivo
In vivo The oral bioavailability of fosfomycin trometamol ranges between 34 and 58%. Absorption occurs in the small intestine, and evidence suggests that coadministration of fosfomycin trometamol with food may reduce absorption of the drug.The mean serum elimination half-life (t1/2) of fosfomycin trometamol is estimated at 5.7 h. Fosfomycin is excreted nonmetabolized in the urine, through glomerular filtration. Depending on age, fasting, and renal function, 11 to 60% of the drug can be found in the urine within 24 h from administration. Specifically, older age, administration with a meal, and deteriorating renal function result in slower elimination through the kidneys. Fosfomycin crosses the blood-brain barrier, and meningeal inflammation increases its concentration in the CSF. Fosfomycin also penetrates in both cortical and cancellous bone, and penetration correlates with plasma levels and the presence of inflammation[2].
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT02654496 Completed
Obesity
North Dakota State University
 January 2016 --
NCT01865812 Completed
Primary Biliary Cirrhosis
Intercept Pharmaceuticals
 December 3 2013 Phase 2
NCT00596427 Completed
Diabetes
Carine Beysen|KineMed
 November 2007 Not Applicable
NCT01589523 Completed
Bile Acid Synthesis Defect|Inborn Error of Bile Acid Metabolism|Inborn Error of Bile Acid Conjugation
Children''s Hospital Medical Center Cincinnati
 February 2006 Phase 3

Chemical Information & Solubility

Molecular Weight 408.57 Formula

C24H40O5
CAS No. 81-25-4 SDF Download Cholic acid SDF
Smiles CC(CCC(=O)O)C1CCC2C1(C(CC3C2C(CC4C3(CCC(C4)O)C)O)O)C
Storage (From the date of receipt)

In vitro
Batch:

DMSO : 81 mg/mL ( (198.25 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 28 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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