Name: Vinblastine sulfate
Brand: Selleck Chemicals
SKU: S4505
Availability: InStock
Rating: 5 (44 reviews)

Jump to

Quality Control

Batch: Purity: 99.91%

99.91

Related Targets	Akt Wnt/beta-catenin PKC HSP ROCK Microtubule Associated Integrin Bcr-Abl Actin FAK
Other Antineoplastic and Immunosuppressive Antibiotics Inhibitors	Staurosporine (STS) Cyclosporin A Oligomycin A (MCH 32) Puromycin Dihydrochloride Nigericin sodium salt Geldanamycin (NSC 122750) Honokiol Streptozotocin (STZ) Sodium Monensin (NSC 343257) Cephalomannine

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description
COLO 320 human colorectal carcinoma cell line	Function assay			In vitro concentration required to kill 50% of COLO 320 human colorectal carcinoma cell line, EC50=0.08 μM
LNCaP human prostate cancer cell line	Function assay			In vitro concentration required to kill 50% of LNCaP human prostate cancer cell line, EC50=0.5 μM
K562 cell	Growth inhibition assay			In vitro inhibitory concentration against human chronic myelogenous leukemia K562 cell growth, IC50=0.001 μM
T47D cells	Function assay			In vitro concentration required to kill 50% of T47D human breast ductal carcinoma cell line, EC50=0.08 μM
K562 cell	Proliferation assay		48 h	Antiproliferative activity against human K562 cells after 48 hrs, IC50=0.001 μM
ACHN cells	Cytotoxicity assay		48 h	Cytotoxicity against human ACHN cells after 48 hrs by SRB assay, IC50=22.7 μM
A375 cells	Cytotoxicity assay		48 h	Cytotoxicity against human A375 cells after 48 hrs by SRB assay, IC50=7.2 μM
C32 cells	Cytotoxicity assay		48 h	Cytotoxicity against human C32 cells after 48 hrs by SRB assay, IC50=3 μM
LNCAP cells	Cytotoxicity assay		48 h	Cytotoxicity against human LNCAP cells after 48 hrs by SRB assay, IC50=29.3 μM
Huh-7D12 cells	Cytotoxicity assay		48 h	Cytotoxicity against human Huh-7D12 cells after 48 hrs by SRB assay, IC50=45.6 μM
COR-L23 cells	Cytotoxicity assay		48 h	Cytotoxicity against human COR-L23 cells after 48 hrs by SRB assay, IC50=45.5 μM
142BR cells	Cytotoxicity assay		48 h	Cytotoxicity against human 142BR cells after 48 hrs by SRB assay, IC50=37.6 μM
HT-29 cells	Cytotoxicity assay		48 h	Cytotoxicity against human HT-29 cells after 48 hrs by MTS assay, IC50=0.55 μM
A549 cells	Proliferation assay		48 h	Antiproliferative activity against human A549 cells after 48 hrs by MTS assay, IC50=2.36 μM
DU145 cells	Proliferation assay		48 h	Antiproliferative activity against human DU145 cells after 48 hrs by MTS assay, IC50=4.25 μM
SK-MEL-5 cells	Proliferation assay		48 h	Antiproliferative activity against human SK-MEL-5 cells after 48 hrs by MTS assay, IC50=1.74 μM
HepG2 cells	Proliferation assay		48 h	Antiproliferative activity against human HepG2 cells after 48 hrs by MTS assay, IC50=0.16 μM
HT-29 cells	Proliferation assay		48 h	Antiproliferative activity against human HT-29 cells after 48 hrs by MTS assay, IC50=11.18 μM
MCF7 cells	Proliferation assay		48 h	Antiproliferative activity against human MCF7 cells after 48 hrs by MTS assay, IC50=24.08 μM
MDA-MB-231 cells	Proliferation assay		48 h	Antiproliferative activity against human MDA-MB-231 cells after 48 hrs by MTS assay, IC50=31.52 μM
rat REF52 cells	Function assay	0.1 μM	30 mins	Induction of microtubule depolymerization in rat REF52 cells at 0.1 uM after 30 mins by fluorescence assay
HepG2 cells	Cytotoxicity assay		72 h	Cytotoxicity against adriamycin-resistant human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.056 μM
HepG2 cells	Cytotoxicity assay		72 h	Cytotoxicity against human HepG2 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.019 μM
K562 cells	Cytotoxicity assay		72 h	Cytotoxicity against human K562 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.016 μM
MDA-MB-231 cells	Cytotoxicity assay		72 h	Cytotoxicity against human MDA-MB-231 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.0083 μM
MCF7 cells	Cytotoxicity assay		72 h	Cytotoxicity against human MCF7 cells assessed as growth inhibition after 72 hrs by MTT assay, IC50=0.007 μM
SCL6 cells	Cytotoxicity assay			Cytotoxicity against human SCL6 cells by MTT assay, ED50=6.1 Μm
SCL9	Cytotoxicity assay			Cytotoxicity against human SCL9 cells by MTT assay, ED=5.3 μM
KATO III cells	Cytotoxicity assay			Cytotoxicity against human KATO III cells by MTT assay, ED50=6.1 μM
NUGC4 cells	Cytotoxicity assay			Cytotoxicity against human NUGC4 cells by MTT assay, ED50=5.3 μM
UACC903 cells	Cytotoxicity assay		48 h	Cytotoxicity against human UACC903 cells after 48 hrs by MTS assay, IC50=1.65 μM
K562 cells	Function assay			Inhibition of tubulin polymerization in human K562 cells, IC50=0.13 μM
BxPC3 cells	Proliferation assay		48 h	Antiproliferative activity against human BxPC3 cells after 48 hrs by MTS assay, IC50=1.13 μM
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

DMSO : 100 mg/mL (110.0 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 50 mg/mL

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	1.670mg/ml (1.84mM)	Taking the 1 mL working solution as an example, add 50 μL of 33.3 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	909.05	Formula	C₄₆H₅₈N₄O₉.H₂SO₄	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	143-67-9	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	NSC-49842, Vincaleukoblastine, 29060-LE			Smiles	CCC1(CC2CC(C3=C(CCN(C2)C1)C4=CC=CC=C4N3)(C5=C(C=C6C(=C5)C78CCN9C7C(C=CC9)(C(C(C8N6C)(C(=O)OC)O)OC(=O)C)CC)OC)C(=O)OC)O.OS(=O)(=O)O

Mechanism of Action

Targets/IC₅₀/Ki	nAChR (Adrenal Chromaffin Cells) 8.9 μM
In vitro	The average terminal half-lives of Vinblastine sulfate is 14.3 h. When incubated in freshly isolated rat hepatocytes, this compound penetrates rapidly and intensely into the cells, probably through a passive diffusion mechanism followed by tight cellular binding. It inhibits the angiogenic response induced by adrenomedullin and is also positive for mitotic slippage, causing micronuclei in mononucleate cells with cytokinesis block. This chemical gives significant increase in micronucleated mononucleated cells at concentrations that produced approximately 50% cell death and cytostasis or less as calculated using RPD, RICC and RCC.
In vivo	Vinblastine is a widely used anticancer drug with undesired side effects . A combination of this compound and RAP at very low doses against human HCC gets a satisfactory antiangiogenic effect in vivo. The clinically relevant dose of this chemical inhibits palmitoylation of tubulin in vivo in CEM cells (effect on depalmitoylation of tubulin).
References	[1] https://pubmed.ncbi.nlm.nih.gov/8516349/ [2] https://pubmed.ncbi.nlm.nih.gov/19666138/ [3] https://pubmed.ncbi.nlm.nih.gov/2088769/ [4] https://pubmed.ncbi.nlm.nih.gov/14508526/ [5] https://pubmed.ncbi.nlm.nih.gov/17202812/

Applications

Methods	Biomarkers	PMID
Western blot	GRP78 p-eIF2 p-JNK / c-Caspase-7 / c-PARP ERK / p-ERK / Mcl-1 / Bad / Bid / Noxa	19674193
Immunofluorescence	α-tubulin / Acetyl tubulin	30120268
Growth inhibition assay	Cell viability	27114800