Xanthoxyline

Catalog No.S4781 Synonyms: Brevifolin

For research use only.

Xanthoxylin (Brevifolin), isolated from Zanthoxylum piperitum (Japanese pepper tree) and Sapium sebiferum (Chinese tallowtree), is a cytotoxic and fungicidal compound with the characteristics of a typical phytoalexin.

Xanthoxyline Chemical Structure

CAS No. 90-24-4

Purity & Quality Control

Related Other Products

Biological Activity

Description Xanthoxylin (Brevifolin), isolated from Zanthoxylum piperitum (Japanese pepper tree) and Sapium sebiferum (Chinese tallowtree), is a cytotoxic and fungicidal compound with the characteristics of a typical phytoalexin.
In vitro

Xanthoxylin (RCX) exhibits potent cytotoxicity in a panel of different cancer cells. It presents IC50 values of 1.6 and 26.0 μM for the HCT116 and ACP-03 cancer cell lines, respectively. Xanthoxylin causes apoptosis in a time- and concentration-dependent manner and induces mitochondrial depolarization in HepG2 cells. It induces DNA intercalation, inhibited DNA synthesis and triggered the caspase-mediated apoptosis pathway in HepG2 cells, as observed by cell shrinkage, internucleosomal DNA fragmentation, externalization of phosphatidylserine, loss of mitochondrial transmembrane potential and activation of caspase-3, -8 and -9[1]. Xanthoxylin increases melanin production, number of dendrites, tyrosinase, and microphthalmia-associated transcription factor (MITF) expression in cultured B16F10 cells[2].

In vivo Xanthoxylin presents a potent in vivo antitumor effect in C.B-17 SCID mice engrafted with HepG2 cells[1].

Protocol (from reference)

Cell Research:[1]
  • Cell lines: HepG2 cells
  • Concentrations: 12 and 24 μM
  • Incubation Time: 12, 24, 48 or 72 h
  • Method: The cell viability of HepG2 cells is determined by the trypan blue staining after 12, 24, 48 and 72 h of incubation.
  • (Only for Reference)
Animal Research:[1]
  • Animal Models: C.B-17 severe combined immunodeficient (SCID) mice engrafted with HepG2 cells
  • Dosages: 2.5 and 5 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 39 mg/mL
(198.77 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 196.20
Formula

C10H12O4

CAS No. 90-24-4
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC(=O)C1=C(C=C(C=C1OC)OC)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Xanthoxyline | Xanthoxyline supplier | purchase Xanthoxyline | Xanthoxyline cost | Xanthoxyline manufacturer | order Xanthoxyline | Xanthoxyline distributor