Name: Thimerosal
Brand: Selleck Chemicals
SKU: S3646
Rating: 5 (2 reviews)

Thimerosal (Thiomersalate, Mercurothiolate) is a well-established antiseptic and antifungal agent and usually used as a preservative in vaccines, immunoglobulin preparations, skin test antigens, antivenins, ophthalmic and nasal products, and tattoo inks. This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.

Thimerosal Chemical Structure

CAS: 54-64-8

Selleck's Thimerosal has been cited by 2 publications

Purity & Quality Control

Batch: S364601 DMSO] 80 mg/mL] false] Water] 80 mg/mL] false] Ethanol] 20 mg/mL] false Purity: 98.31%

98.31

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Biological Activity

Description

Thimerosal (Thiomersalate, Mercurothiolate) is a well-established antiseptic and antifungal agent and usually used as a preservative in vaccines, immunoglobulin preparations, skin test antigens, antivenins, ophthalmic and nasal products, and tattoo inks. This product is a hazardous chemical (acute toxicity/flammable/skin corrosive). Please use it while wearing a protective face mask, gloves, and clothing.

In vitro
In vitro	The treatment of Jurkat T cells with thimerosal causes a significant decrease in cellular viability at 1 μM (25%, p<0.05; IC50: 10 μM)^[1]. Thimerosal is also able to induce apoptosis and G2/M phase in human leukemia U937 cells^[2].
Cell Research	Cell lines	Jurkat T cells
	Concentrations	0.01, 0.1, 1, 10, 50, 100, 250 μM
	Incubation Time	48 h
	Method	The number of 1×10⁴ cells/well Jurkat T cells are seeded into 96 well plates and exposed to thimerosal, methyl mercury, and mercuric chloride (HgCl2) at concentrations of thimerosal (0.01-0.1-1-10-50-100-250 μM), methyl mercury (30-50-80-100-250 μM), and mercuric chloride (HgCl2 (20-40-60-80-100 μM). The plates are incubated at 37°C in a humidified atmosphere containing 5% CO2. After 48 hours, the medium is discarded and 20 μl/well of MTT solution (5 mg/ml) is added and incubated for 3 hours at 37°C (5% CO2). Finally, 20μl/well of isopropanol is added and the color intensity is read spectrophotometrically at 590 nm.

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
Clinical Trial Information (data from https://clinicaltrials.gov, updated on 2024-01-11)
NCT00795951	Completed	Dermatitis Contact	Allerderm	November 2008	Phase 4

References

Chemical Information & Solubility

Molecular Weight	404.81	Formula	C₉H₉HgO₂S.Na
CAS No.	54-64-8	SDF	Download Thimerosal SDF
Smiles	CC[Hg]SC1=CC=CC=C1C(=O)[O-].[Na+]
Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
Storage (From the date of receipt)	3 years-20°Cpowder		1 month-20°Cin solvent

In vitro
Batch:

DMSO : 80 mg/mL ( (197.62 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : 80 mg/mL

Ethanol : 20 mg/mL

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In vivo
Batch:

Add solvents to the product individually and in order.

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In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

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% DMSO + % + % Tween 80 + % ddH₂O

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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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