WIN 55, 212-2 mesylate

For research use only.

Catalog No.S8017 Synonyms: (R)-(+)-WIN 55212

WIN 55, 212-2 mesylate Chemical Structure

CAS No. 131543-23-2

WIN 55,212-2 Mesylate ((R)-(+)-WIN 55212) is a potent cannabinoid (CB) receptor agonist with Ki of 3.3 nM and 62.3 nM for human recombinant CB2 and CB1, respectively. WIN 55,212-2 Mesylate regulates Glutamate transmission.

Purity & Quality Control

Choose Selective Cannabinoid Receptor Inhibitors

Biological Activity

Description WIN 55,212-2 Mesylate ((R)-(+)-WIN 55212) is a potent cannabinoid (CB) receptor agonist with Ki of 3.3 nM and 62.3 nM for human recombinant CB2 and CB1, respectively. WIN 55,212-2 Mesylate regulates Glutamate transmission.
Targets
CB2 [2]
(Cell-free assay)
CB1 [2]
(Cell-free assay)
3.3 nM(Ki) 62.3 nM(Ki)
In vitro

WIN 55,212-2 increases, in a nanomolar concentration range, extra-cellular glutamate levels in primary cultures of rat cerebral cortex neurons, displaying a bell-shaped concentration-response curve.[1] The aminoalkylindole WIN 55,212-2 is more selective for the CB2 than the CB1 receptor, with a CB2-to-CB1 dose-response ratio of 19.[2]

In vivo

The result provide in vivo evidence for CB1 receptor-mediated increase on endogenous glutamate levels in the cerebral cortex. The cannabinoid receptor agonist WIN 55,212-2, at low mg/kg doses, enhances dialysate glutamate levels in the prefrontal cortex of the awake rat.[1]

Protocol

Cell Research:

[1]

- Collapse
  • Cell lines: cerebral cortical cells
  • Concentrations: 1 nM, 10 nM
  • Incubation Time: 15 min
  • Method:

    On the day of the release experiment, the cells are rinsed twice by replacing the culture medium with Krebs Ringer-bicarbonate buffer (37℃). Thereafter, five consecutive fractions are collected, renewing this solution (400μl) every 30 min. The first 2 samples are used to assess basal glutamate levels, while pharmacological treatments are carried out during the third fraction. WIN 55,212-2 is applied 15 min before the end of the third fraction.


    (Only for Reference)
Animal Research:

[1]

- Collapse
  • Animal Models: male adult sprague-dawley rats
  • Dosages: 0.01 mg/kg - 2 mg/kg
  • Administration: IP
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 100 mg/mL (191.34 mM)
Water 100 mg/mL (191.34 mM)
Ethanol 20 mg/mL (38.26 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 522.61
Formula

C28H30N2O6S

Density g/mL
CAS No. 131543-23-2
Storage powder
in solvent
Synonyms (R)-(+)-WIN 55212
Smiles CC1=C(C2=C3N1C(COC3=CC=C2)CN4CCOCC4)C(=O)C5=CC=CC6=CC=CC=C65.CS(=O)(=O)O

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)
Dosage mg/kg Average weight of animals g Dosing volume per animal ul Number of animals
Step 2: Enter the in vivo formulation ()
% DMSO % % Tween 80 % ddH2O
CalculateReset

Bio Calculators

Molarity Calculator

Molarity Calculator

Calculate the mass, volume or concentration required for a solution. The Selleck molarity calculator is based on the following equation:

Mass (mg) = Concentration (mM) × Volume (mL) × Molecular Weight (g/mol)

  • Mass
    Concentration
    Volume
    Molecular Weight

*When preparing stock solutions, please always use the batch-specific molecular weight of the product found on the via label and SDS / COA (available on product pages).

Dilution Calculator

Dilution Calculator

Calculate the dilution required to prepare a stock solution. The Selleck dilution calculator is based on the following equation:

Concentration (start) x Volume (start) = Concentration (final) x Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2 ( Input Output )

  • C1
    V1
    C2
    V2

* When preparing stock solutions always use the batch-specific molecular weight of the product found on the vial label and SDS / COA (available online).

The Serial Dilution Calculator Equation

  • Serial Dilutions

  • Computed Result

  • C1=C0/X C1: LOG(C1):
    C2=C1/X C2: LOG(C2):
    C3=C2/X C3: LOG(C3):
    C4=C3/X C4: LOG(C4):
    C5=C4/X C5: LOG(C5):
    C6=C5/X C6: LOG(C6):
    C7=C6/X C7: LOG(C7):
    C8=C7/X C8: LOG(C8):
Molecular Weight Calculator

Molecular Weight Calculator

Enter the chemical formula of a compound to calculate its molar mass and elemental composition:

Total Molecular Weight: g/mol

Tip: Chemical formula is case sensitive. C10H16N2O2 c10h16n2o2

Instructions to calculate molar mass (molecular weight) of a chemical compound:

To calculate molar mass of a chemical compound, please enter its chemical formula and click 'Calculate'.

Definitions of molecular mass, molecular weight, molar mass and molar weight:

Molecular mass (molecular weight) is the mass of one molecule of a substance and is expressed in the unified atomic mass units (u). (1 u is equal to 1/12 the mass of one atom of carbon-12)
Molar mass (molar weight) is the mass of one mole of a substance and is expressed in g/mol.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

  • * Indicates a Required Field

Cannabinoid Receptor Signaling Pathway Map

Related Cannabinoid Receptor Products

Tags: buy WIN 55, 212-2 mesylate | WIN 55, 212-2 mesylate supplier | purchase WIN 55, 212-2 mesylate | WIN 55, 212-2 mesylate cost | WIN 55, 212-2 mesylate manufacturer | order WIN 55, 212-2 mesylate | WIN 55, 212-2 mesylate distributor
×
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID