Usnic acid

Catalog No.S4953 Synonyms: Usniacin

For research use only.

Usnic acid (Usniacin) is a furandione found uniquely in lichen that is used widely in cosmetics, deodorants, toothpaste and medicinal creams as well as some herbal products. It exhibits antiviral, antiprotozoal, antiproliferative, anti-inflammatory and analgesic activity. Usnic acid inhibits breast tumor angiogenesis and growth by suppressing VEGFR2-mediated AKT and ERK1/2 signaling pathways.

Usnic acid Chemical Structure

CAS No. 125-46-2

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Biological Activity

Description Usnic acid (Usniacin) is a furandione found uniquely in lichen that is used widely in cosmetics, deodorants, toothpaste and medicinal creams as well as some herbal products. It exhibits antiviral, antiprotozoal, antiproliferative, anti-inflammatory and analgesic activity. Usnic acid inhibits breast tumor angiogenesis and growth by suppressing VEGFR2-mediated AKT and ERK1/2 signaling pathways.
Targets
Akt [3] ERK [3]
In vitro

Usnic acid inhibits cell growth involving G0/G1 phase cell cycle arrest and induces cell death via mitochondrial membrane depolarization and induction of apoptosis in human lung carcinoma cells. Usnic acid has shown activity againstmany cancer cells such as lung carcinoma cells, murine leukemia P388 and L1210 cells, colon carcinoma HT-29 and HCT-116 cells, breast adenocarcinoma MCF-7 cells, and cervix adenocarcinoma cells[1]. Usnic acid also selectively inhibits the viability of human breast cancer MCF-7 cells in a concentration- and time-dependent manner, provoking the generation of reactive oxygen species (ROS), which triggers the mitochondrial/caspase apoptotic pathway in MCF-7 cells[2].

In vivo

In an MCF-7 xenograft model, Usnic acid significantly suppresses the growth of tumors by inducing apoptosis in the tumor cells without overt toxic side effects[2].

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: A549 cells
  • Concentrations: 25, 50, and 100 μM
  • Incubation Time: 24 and 48 h
  • Method:

    Cells at a density of 1 × 105 are seeded per 60-mm culture plate and treated with either DMSO or indicated concentrations of usnic acid for 24 and 48 h. After each treatment time, cells are harvested by brief trypsinization and then gently mixed with trypan blue and counted using hemocytometer.

Animal Research:

[2]

  • Animal Models: 6- to 8-week-old female BALB/c nu/nu mice (subcutaneously injected with MCF-7 cells)
  • Dosages: 25, 50 or 100 mg/kg
  • Administration: i.p.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 344.32
Formula

C18H16O7

CAS No. 125-46-2
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles CC1=C(C(=C2C(=C1O)C3(C(=CC(=O)C(C3=O)C(=O)C)O2)C)C(=O)C)O

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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