SJ-172550

Catalog No.E0051

For research use only.

SJ 172550 is the first MDMX inhibitor that binds reversibly to MDMX and effectively kills retinoblastoma cells in which the expression of MDMX is amplified.

SJ-172550 Chemical Structure

CAS No. 431979-47-4

Purity & Quality Control

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Biological Activity

Description SJ 172550 is the first MDMX inhibitor that binds reversibly to MDMX and effectively kills retinoblastoma cells in which the expression of MDMX is amplified.
In vitro

SJ-172550 binds reversibly to MDMX and effectively kills retinoblastoma cells in which the expression of MDMX is amplified. The effect of SJ-172550 is additive when combined with an MDM2 inhibitor nutlin-3a.<sup><a class="sref" href="#s_ref">[1]</a></sup>

Protocol (from reference)

Cell Research:

<sup><a class="sref" href="#s_ref">[1]</a></sup>

  • Cell lines: Retinoblastoma cells, ML-1 leukemia cells
  • Concentrations: 20 μM
  • Incubation Time: 20 h
  • Method:

    Retinoblastoma cells and ML-1 leukemia cells (with wild-type p53) are exposed to SJ-172550 (20 μm) for 20 h. And then the p53 and activated caspase-3 levels are analyzed by immunofluorescence. Nutlin-3a (5 μm; 20 h) is used for as the positive controls. DMSO is used as the negative control.

Solubility (25°C)

In vitro

DMSO 86 mg/mL
(200.52 mM)
Water Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 428.87
Formula

C₂₂H₂₁ClN₂O₅

CAS No. 431979-47-4
Storage 3 years -20°C powder
2 years -80°C in solvent

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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