SJ-172550

Catalog No.E0051 Batch:E005101

Technical Data

Formula	C₂₂H₂₁ClN₂O₅
Molecular Weight	428.87	CAS No.	431979-47-4
Solubility (25°C)*	In vitro	DMSO	86 mg/mL (200.52 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

SJ 172550 is the first MDMX inhibitor that binds reversibly to MDMX and effectively kills retinoblastoma cells in which the expression of MDMX is amplified.

Targets

MDMX ^[1]

In vitro

SJ-172550 binds reversibly to MDMX and effectively kills retinoblastoma cells in which the expression of MDMX is amplified. The effect of SJ-172550 is additive when combined with an MDM2 inhibitor nutlin-3a.^[1]

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Retinoblastoma cells, ML-1 leukemia cells Concentrations 20 μM Incubation Time 20 h Method Retinoblastoma cells and ML-1 leukemia cells (with wild-type p53) are exposed to SJ-172550 (20 μm) for 20 h. And then the p53 and activated caspase-3 levels are analyzed by immunofluorescence. Nutlin-3a (5 μm; 20 h) is used for as the positive controls. DMSO is used as the negative control.

Cell Assay:^{^[1]}

Cell lines
Retinoblastoma cells, ML-1 leukemia cells
Concentrations
20 μM
Incubation Time
20 h
Method
Retinoblastoma cells and ML-1 leukemia cells (with wild-type p53) are exposed to SJ-172550 (20 μm) for 20 h. And then the p53 and activated caspase-3 levels are analyzed by immunofluorescence. Nutlin-3a (5 μm; 20 h) is used for as the positive controls. DMSO is used as the negative control.

References

[1] Reed D, et al. J Biol Chem. 2010 Apr 2;285(14):10786-96.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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