PS-48

Catalog No.S7586

For research use only.

PS-48 is an allosteric activator of phosphoinositide-dependent protein kinase-1 (PDK1).

PS-48 Chemical Structure

CAS No. 1180676-32-7

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Biological Activity

Description PS-48 is an allosteric activator of phosphoinositide-dependent protein kinase-1 (PDK1).
Targets
PDK1 [1]
()
In vitro

The PDK1 agonist PS48 is able to promote the osteogenic ability of MC3T3-E1 cells treated with dexamethasone. The protein levels of p-AKT and p-mTOR are increased in MC3T3-E1 cells treated with dexamethasone after PS48 treatment.[2]

In vivo

In vivo, the PDK1 agonist PS48 can maintain the bone mass of mice treated with dexamethasone.[2]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: MC3T3-E1 cells
  • Concentrations: 5 μM
  • Incubation Time: --
  • Method:

    MC3T3-E1 cells are cultured in an osteogenic differentiation medium containing 4 mM glycerophosphate and 25 μg/mL ascorbic acid until 70% confluency. Dexamethasone (final concentration of ethanol, 0.01%, vol/vol) at different concentrations is then added to the osteogenic differentiation medium for 14 days. The culture medium is replaced every two days. MC3T3-E1 cells are cultured in a culture medium containing 10−7 M dexamethasone supplemented with or without PS48 (5 μM).

Animal Research:

[2]

  • Animal Models: 8-week-old male C57BL/6J mice
  • Dosages: --
  • Administration: --

Solubility (25°C)

In vitro

DMSO 57 mg/mL
(198.77 mM)
Water Insoluble
Ethanol '''57 mg/mL

Chemical Information

Molecular Weight 286.75
Formula

C17H15ClO2

CAS No. 1180676-32-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC=C(C=C1)C(=CC(=O)O)CCC2=CC=C(C=C2)Cl

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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