PS-48

For research use only.

Catalog No.S7586

PS-48 Chemical Structure

CAS No. 1180676-32-7

PS-48 is an allosteric activator of phosphoinositide-dependent protein kinase-1 (PDK1).

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Biological Activity

Description PS-48 is an allosteric activator of phosphoinositide-dependent protein kinase-1 (PDK1).
Targets
PDK1 [1]
()
In vitro

The PDK1 agonist PS48 is able to promote the osteogenic ability of MC3T3-E1 cells treated with dexamethasone. The protein levels of p-AKT and p-mTOR are increased in MC3T3-E1 cells treated with dexamethasone after PS48 treatment.[2]

In vivo

In vivo, the PDK1 agonist PS48 can maintain the bone mass of mice treated with dexamethasone.[2]

Protocol

Cell Research:

[2]

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  • Cell lines: MC3T3-E1 cells
  • Concentrations: 5 μM
  • Incubation Time: --
  • Method:

    MC3T3-E1 cells are cultured in an osteogenic differentiation medium containing 4 mM glycerophosphate and 25 μg/mL ascorbic acid until 70% confluency. Dexamethasone (final concentration of ethanol, 0.01%, vol/vol) at different concentrations is then added to the osteogenic differentiation medium for 14 days. The culture medium is replaced every two days. MC3T3-E1 cells are cultured in a culture medium containing 10−7 M dexamethasone supplemented with or without PS48 (5 μM).


    (Only for Reference)
Animal Research:

[2]

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  • Animal Models: 8-week-old male C57BL/6J mice
  • Dosages: --
  • Administration: --
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 57 mg/mL (198.77 mM)
Water Insoluble
Ethanol '''57 mg/mL

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 286.75
Formula

C17H15ClO2

CAS No. 1180676-32-7
Storage powder
in solvent
Synonyms N/A
Smiles C1=CC=C(C=C1)C(=CC(=O)O)CCC2=CC=C(C=C2)Cl

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PDK Signaling Pathway Map

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID