PS-48

Catalog No.S7586 Batch:S758601

Technical Data

Formula	C₁₇H₁₅ClO₂
Molecular Weight	286.75	CAS No.	1180676-32-7
Solubility (25°C)*	In vitro	DMSO	57 mg/mL (198.77 mM)
		Ethanol	57 mg/mL (198.77 mM)
		Water	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description

PS-48 is an allosteric activator of phosphoinositide-dependent protein kinase-1 (PDK1).

Targets

PDPK1 ^[1]

In vitro

The PDK1 agonist PS48 is able to promote the osteogenic ability of MC3T3-E1 cells treated with dexamethasone. The protein levels of p-AKT and p-mTOR are increased in MC3T3-E1 cells treated with dexamethasone after PS48 treatment.^[2]

In vivo

In vivo, the PDK1 agonist PS48 can maintain the bone mass of mice treated with dexamethasone.^[2]

Protocol (from reference)

Cell Assay:^{^[2]}	Cell lines MC3T3-E1 cells Concentrations 5 μM Incubation Time -- Method MC3T3-E1 cells are cultured in an osteogenic differentiation medium containing 4 mM glycerophosphate and 25 μg/mL ascorbic acid until 70% confluency. Dexamethasone (final concentration of ethanol, 0.01%, vol/vol) at different concentrations is then added to the osteogenic differentiation medium for 14 days. The culture medium is replaced every two days. MC3T3-E1 cells are cultured in a culture medium containing 10−7 M dexamethasone supplemented with or without PS48 (5 μM).
Animal Study:^{^[2]}	Animal Models 8-week-old male C57BL/6J mice Dosages -- Administration --

Cell Assay:^{^[2]}

Cell lines
MC3T3-E1 cells
Concentrations
5 μM
Incubation Time
--
Method
MC3T3-E1 cells are cultured in an osteogenic differentiation medium containing 4 mM glycerophosphate and 25 μg/mL ascorbic acid until 70% confluency. Dexamethasone (final concentration of ethanol, 0.01%, vol/vol) at different concentrations is then added to the osteogenic differentiation medium for 14 days. The culture medium is replaced every two days. MC3T3-E1 cells are cultured in a culture medium containing 10−7 M dexamethasone supplemented with or without PS48 (5 μM).

Animal Study:^{^[2]}

Animal Models
8-week-old male C57BL/6J mice
Dosages
--
Administration
--

References

Selleck's PS-48 has been cited by 1 publication

Direct chemical reprogramming of human cord blood erythroblasts to induced megakaryocytes that produce platelets [ Cell Stem Cell, 2022, 29(8):1229-1245.e7]	PubMed: 35931032

Direct chemical reprogramming of human cord blood erythroblasts to induced megakaryocytes that produce platelets [ Cell Stem Cell, 2022, 29(8):1229-1245.e7]

PubMed: 35931032

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

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