OT-82

Catalog No.S9706

For research use only.

OT-82 is a novel nicotinamide phosphoribosyltransferase (NAMPT) inhibitor with average IC50s of 13.03   nM in non-HP cancer cells and 2.89   nM in HP cancer cells, respectively.

OT-82 Chemical Structure

CAS No. 1800487-55-1

Purity & Quality Control

Choose Selective NAMPT Inhibitors

Biological Activity

Description OT-82 is a novel nicotinamide phosphoribosyltransferase (NAMPT) inhibitor with average IC50s of 13.03   nM in non-HP cancer cells and 2.89   nM in HP cancer cells, respectively.
Targets
NAMPT [1]
(in hematopoietic cells)
2.89 nM
In vitro

OT-82 is an effective nicotinamide phosphoribosyltransferase (NAMPT) inhibitor, thus inhibits the salvage pathway of NAD synthesis to kill the neoplastic cells of hematopoietic. The average IC50 is 13.03 nM in non-HP cancer cells and 2.89 nM in HP cancer cells.<sup><a class="sref" href="#s_ref">[1]</a></sup>

In vivo

OT-82 has therapeutic effect in subcutaneous(SC) and systemic mouse xenograft models of HP malignancies.<sup><a class="sref" href="#s_ref">[1]</a></sup>

Protocol (from reference)

Cell Research:

<sup><a class="sref" href="#s_ref">[1]</a></sup>

  • Cell lines: MV4–11 cells
  • Concentrations: --
  • Incubation Time: 1 h
  • Method:

    MV4 11 cell extract (3 mg protein/reaction) was incubated for 1 h at 40 ℃ with Affi-Gel 10 coupled with "active" compound (OT-82), "inactive" compound (a non-cytotoxic OT- 82 structural analog), or a combination of the two ("competition"). Eluted proteins were subjected to gel electrophoresis and silver staining or transferred to nitrocellulose membrane for western blotting with NAMPT antisera.

Animal Research:

<sup><a class="sref" href="#s_ref">[1]</a></sup>

  • Animal Models: Ubcutaneous (SC) and systemic mouse xenograft models of HP malignancies
  • Dosages: 25 or 50 mg/kg for 6 consecutive days per week for 3 weeks
  • Administration: p.o.

Solubility (25°C)

In vitro

DMSO 85 mg/mL
(200.24 mM)
Water Insoluble
Ethanol ''85 mg/mL

Chemical Information

Molecular Weight 424.47
Formula

C26H21FN4O

CAS No. 1800487-55-1
Storage 3 years -20°C powder
2 years -80°C in solvent

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Molarity Calculator

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Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT03921879 Recruiting Drug: OT-82 Dose Escalation|Drug: OT-82 Dose Expansion Lymphoma|Lymphoma Non-Hodgkin|Lymphoma B-Cell|Lymphoma T-Cell|Lymphoma Follicular|Lymphoma Peripheral T-Cell|Lymphoma Hodgkin Oncotartis Inc. July 29 2019 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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