(-)-Arctigenin

Catalog No.S3897

For research use only.

(-)-Arctigenin, a lignan found in certain plants of the Asteraceae, exerts biological activities including anti-inflammatory, antiviral and anticancer.

(-)-Arctigenin Chemical Structure

CAS No. 7770-78-7

Purity & Quality Control

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Biological Activity

Description (-)-Arctigenin, a lignan found in certain plants of the Asteraceae, exerts biological activities including anti-inflammatory, antiviral and anticancer.
In vitro

Arctigenin induces apoptosis in HT-29 colon cancer cells by regulating ROS and p38 MAPK pathways[1]. It is a phytoestrogen that exerts a proliferative effect by binding to the ER. Treatment with arctigenin effectively downregulates ERα expression. Thus, arctigenin, mainly targets the mTOR pathway in ERα-positive MCF-7 human breast cancer cells, leading to autophagy-induced cell death and the downregulation of ERα expression[2]. Arctigenin decreases BACE1 translation via dephosphorylation of eIF2α, causing the suppression of Aβ production, and initiates autophagy through both AMPK/Raptor signaling activation and AKT/mTOR pathway inhibition, resulting in enhanced Aβ clearance. Arctigenin suppresses BACE1 activity via inhibition of BACE1 translation[3].

In vivo Treatment of arctigenin in mice highly decreases Aβ formation and senile plaques and efficiently ameliorates AD mouse memory impairment of APP/PS1 transgenic model mice[3].

Protocol (from reference)

Cell Research:[3]
  • Cell lines: HEK293-APPswe, primary cortical neuronal, and BV2 cells
  • Concentrations: 0.1, 1, 10, 20, 40 μM
  • Incubation Time: 24 h
  • Method: Cells are treated with 0.1% DMSO or different concentrations of arctigenin for 24 h. The cell culture media are collected, and a complete protease inhibitor mixture is added. The culture media are then centrifuged by 20,000 × g at 4°C for 30 min, and supernatants are collected.
  • (Only for Reference)
Animal Research:[3]
  • Animal Models: The APP/PS1 mice
  • Dosages: 3 mg/kg/d
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 74 mg/mL
(198.7 mM)


* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 372.41
Formula

C21H24O6

CAS No. 7770-78-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles COC1=C(C=C(C=C1)CC2COC(=O)C2CC3=CC(=C(C=C3)O)OC)OC

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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