Home Microbiology HBV inhibitor Morphothiadin For research use only.

Morphothiadin

Synonyms: GLS4

Morphothiadin (GLS4) is a potent inhibitor on the replication of both wild-type and adefovir-resistant Hepatitis B virus (HBV) with an IC50 of 12 nM. Morphothiadin (GLS4), a nucleoside analogs that target the virus polymerase, can block the HBV replication cycle and HBV-associated chronic liver diseases.

Morphothiadin Chemical Structure

Morphothiadin Chemical Structure

CAS: 1092970-12-1

Purity & Quality Control

Batch: E009401 DMSO] 100 mg/mL] false] Ethanol] 12 mg/mL] false] Water] Insoluble] false Purity: 99.87%
99.87

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Biological Activity

Description Morphothiadin (GLS4) is a potent inhibitor on the replication of both wild-type and adefovir-resistant Hepatitis B virus (HBV) with an IC50 of 12 nM. Morphothiadin (GLS4), a nucleoside analogs that target the virus polymerase, can block the HBV replication cycle and HBV-associated chronic liver diseases.
Targets
HBV [1]
(in the Southern blotting of analysis of replicative intermediate of HBV)
12 nM
In vitro
In vitro

In vitro, Morphothiadin (GLS4) is significantly less toxic for primary human hepatocytes, and inhibits virus accumulation in the supernantant of HepAD38 cells and HBV replicative forms in the liver significantly, also strongly inhibits core gene expression.[1];
Cell Research Cell lines HepAD38 cells
Concentrations 25, 50, 100, 200, 400 nM
Incubation Time 7 days
Method

5×106 HepAD38 cells were seeded into each well of 6-well plates, and the wells were then treated with GLS4 for 7 days. Fresh medium and drug were added daily. Cell supernatants were assayed for the levels of HBV DNA by real-time PCR.
In Vivo
In vivo

For nude mice inoculated with HepAD38 cells, treatment of with GLS4 shows strong and sustained suppression of virus DNA to about the same extents both during and after treatment, and reduces the levels of intracellular core antigen in the tumors.[1]
Animal Research Animal Models Nude mice inoculated with HepAD38 cells
Dosages 3.75 mg/kg
Administration o.g.

Chemical Information & Solubility

Molecular Weight 509.39 Formula

C21H22BrFN4O3S
CAS No. 1092970-12-1 SDF --
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 100 mg/mL ( (196.31 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 12 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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