Morphothiadin

Catalog No.E0094 Batch:E009401

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Technical Data

Formula

C21H22BrFN4O3S
Molecular Weight 509.39 CAS No. 1092970-12-1
Solubility (25°C)* In vitro DMSO 100 mg/mL (196.31 mM)
Ethanol 12 mg/mL (23.55 mM)
Water Insoluble
* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

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Biological Activity

Description Morphothiadin (GLS4) is a potent inhibitor on the replication of both wild-type and adefovir-resistant Hepatitis B virus (HBV) with an IC50 of 12 nM. Morphothiadin (GLS4), a nucleoside analogs that target the virus polymerase, can block the HBV replication cycle and HBV-associated chronic liver diseases.
Targets
HBV [1]
(in the Southern blotting of analysis of replicative intermediate of HBV)
12 nM
In vitro

In vitro, Morphothiadin (GLS4) is significantly less toxic for primary human hepatocytes, and inhibits virus accumulation in the supernantant of HepAD38 cells and HBV replicative forms in the liver significantly, also strongly inhibits core gene expression.[1];
In vivo

For nude mice inoculated with HepAD38 cells, treatment of with GLS4 shows strong and sustained suppression of virus DNA to about the same extents both during and after treatment, and reduces the levels of intracellular core antigen in the tumors.[1]

Protocol (from reference)

Cell Assay:

[1]
  • Cell lines

    HepAD38 cells

  • Concentrations

    25, 50, 100, 200, 400 nM

  • Incubation Time

    7 days

  • Method

    5×106 HepAD38 cells were seeded into each well of 6-well plates, and the wells were then treated with GLS4 for 7 days. Fresh medium and drug were added daily. Cell supernatants were assayed for the levels of HBV DNA by real-time PCR.
Animal Study:

[1]
  • Animal Models

    Nude mice inoculated with HepAD38 cells

  • Dosages

    3.75 mg/kg

  • Administration

    o.g.

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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.