Home ER stress & UPR PDI inhibitor ML359 For research use only.

ML359

ML359 is a small molecule inhibitor of protein disulfide isomerase （PDI).

ML359 Chemical Structure

ML359 Chemical Structure

CAS: 1069858-99-6

Purity & Quality Control

Batch: S284601 DMSO] 84 mg/mL] false] Ethanol] 84 mg/mL] false] Water] Insoluble] false Purity: 99.18%
99.18

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Biological Activity

Description ML359 is a small molecule inhibitor of protein disulfide isomerase （PDI).
Targets
PDI [1]
In vitro
In vitro

ML359 shows no cytotoxicity in three human cell lines (HeLa, HEK293, and HepG2), and some activity in inhibiting platelet aggregation in vitro (25% inhibition at 30 µM).[1]
Cell Research Cell lines HeLa, HEK293, and HepG2 cell lines
Concentrations 0.004-26 µM
Incubation Time 48 h
Method

Cells were treated with compounds for 48 h, and then measured for cell viability, a luciferase-based reagent that measures cellular ATP levels. The compounds were tested at concentrations ranging from 26  M to 0.004  M, to determine IC50 values. Compounds that were inactive (IC50   30 µM) in this assay were considered for probe development. Data were normalized against the neutral control wells (DMSO) and positive control wells (20 uM Staurosporine, CID 44259) in Genedata Screener Assay Analyzer.
In Vivo
In vivo

ML359 inhibits of thrombus formation in a mouse laser injury model.[2]
Animal Research Animal Models mouse laser injury model
Dosages --
Administration --

Chemical Information & Solubility

Molecular Weight 417.93 Formula

C23H28ClNO4
CAS No. 1069858-99-6 SDF --
Smiles CCOC(=O)C1(CCOC2=CC=CC=C2)CCN(CC1)CC3=CC=C(O)C(=C3)Cl
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 84 mg/mL ( (200.99 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 84 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

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Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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