Catalog No.S2846

For research use only.

ML359 is a small molecule inhibitor of protein disulfide isomerase (PDI).

ML359 Chemical Structure

CAS No. 1069858-99-6

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Biological Activity

Description ML359 is a small molecule inhibitor of protein disulfide isomerase (PDI).
PDI [1]
In vitro

ML359 shows no cytotoxicity in three human cell lines (HeLa, HEK293, and HepG2), and some activity in inhibiting platelet aggregation in vitro (25% inhibition at 30 µM).[1]

In vivo

ML359 inhibits of thrombus formation in a mouse laser injury model.[2]

Protocol (from reference)

Cell Research:


  • Cell lines: HeLa, HEK293, and HepG2 cell lines
  • Concentrations: 0.004-26 µM
  • Incubation Time: 48 h
  • Method:

    Cells were treated with compounds for 48 h, and then measured for cell viability, a luciferase-based reagent that measures cellular ATP levels. The compounds were tested at concentrations ranging from 26  M to 0.004  M, to determine IC50 values. Compounds that were inactive (IC50   30 µM) in this assay were considered for probe development. Data were normalized against the neutral control wells (DMSO) and positive control wells (20 uM Staurosporine, CID 44259) in Genedata Screener Assay Analyzer.

Animal Research:


  • Animal Models: mouse laser injury model
  • Dosages: --
  • Administration: --

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 417.93


CAS No. 1069858-99-6
Storage 3 years -20°C powder
2 years -80°C in solvent

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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