Miglustat

Catalog No.E1029 Synonyms: Zavesca, N‐butyldeoxynojirimycin, OGT 918

For research use only.

Miglustat (Zavesca, N‐butyldeoxynojirimycin, OGT 918) is an orally administered ceramide glucosyltransferase inhibitor which prevents the lysosomal accumulation of glucocerebroside.

Miglustat Chemical Structure

CAS No. 72599-27-0

Purity & Quality Control

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Biological Activity

Description

Miglustat (Zavesca, N‐butyldeoxynojirimycin, OGT 918) is an orally administered ceramide glucosyltransferase inhibitor which prevents the lysosomal accumulation of glucocerebroside.

Targets
ceramide glucosyltransferase [1]
()
In vitro

Both HCT116 and Lovo cells show decreased tumor microsphere growth with pronounced growth retardation of Lovo cell spheroids at low concentrations of Miglustat. A marked elevation of SM and PC in is observed in Lovo cells treated with Miglustat.[2]

In vivo

Miglustat treatment of Mcoln1−/− mice successfully delays impairments in cerebellar-mediated motor coordination, Purkinje cell death, and cerebellar microgliosis.[3]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: HCT116, Lovo cells
  • Concentrations: 100 µM
  • Incubation Time: 4 days
  • Method:

    Lovo and HCT116 cells are cultivated in MEM-alpha medium, cells are cultivated in 10 mL culture medium in- or without the presence of 100 µM Miglustat. Cells are cultivated in 10 cm tissue culture dishes for 4 days. Cells are counted, split and treated for another 2 days, each in triplicates. Cells are harvested and used for FACS-, protein-, and TLC-analysis.

  • (Only for Reference)
Animal Research:

[3]

  • Animal Models: Mcoln1−/− mice
  • Dosages: 300 mg/kg
  • Administration: i.p.
  • (Only for Reference)

Solubility (25°C)

In vitro

DMSO 44 mg/mL
(200.65 mM)
Water 44 mg/mL
(200.65 mM)
Ethanol 22 mg/mL
(100.32 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 219.28
Formula

C10H21NO4

CAS No. 72599-27-0
Storage 3 years -20°C powder
2 years -80°C in solvent

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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