Home Metabolism Phospholipase (e.g. PLA) activator Melittin For research use only.

Melittin

Synonyms: MLT, Forapin, Forapine

Melittin (MLT, Forapin, Forapine) is an activator of phospholipase A2 (PLA2) that stimulates the activity of the low molecular weight PLA2, while it does not the increase the activity of the high molecular weight enzyme.

Melittin Chemical Structure

Melittin Chemical Structure

CAS: 20449-79-0, 37231-28-0

Selleck's Melittin has been cited by 1 publication

Purity & Quality Control

Batch: S979401 Water] 100 mg/mL] false] ] ] false] ] ] false Purity: 99.79%
99.79

Melittin Related Products

Signaling Pathway

Phospholipase (e.g. PLA) Signaling Pathways

Phospholipase (e.g. PLA) Signaling Pathway

Choose Selective Phospholipase (e.g. PLA) Inhibitors

Biological Activity

Description Melittin (MLT, Forapin, Forapine) is an activator of phospholipase A2 (PLA2) that stimulates the activity of the low molecular weight PLA2, while it does not the increase the activity of the high molecular weight enzyme.
Targets
PLA2 [1]
In vitro
In vitro

In vitro studies using TNF-α/IFN-γ-stimulated human keratinocytes shows that melittin inhibits the increased expression of chemokines, such as CCL17 and CCL22, and pro-inflammatory cytokines, including IL-1β, IL-6 and IFN-γ, through the blockade of the NF‐κB and STAT signalling pathways.[2]
Cell Research Cell lines HaCaT cell line
Concentrations 0.1 μg/mL, 0.5 μg/mL, 1 μg/mL
Incubation Time 30 min
Method

The human keratinocyte HaCaT cell line is cultured in high glucose DMEM supplemented with 10% (v/v) FBS and antibiotics at 37°C in a humidified 5% CO2 incubator. HaCaT (5.0 × 105 cells/mL) cells are seeded in the complete medium. 24 hours later, the medium is changed to serumfree mediumwith bee venom (1, 10 and 100 ng/mL) and melittin (0.1, 0.5 and 1 μg/mL). After 30 min, the cells are stimulated with 10 ng/mL TNF-α/IFN-γ for 9 h.
In Vivo
In vivo

Melittin exhibits potent anti-atopic activities, shown by decreased Atopic dermatitis (AD)-like skin lesions, induced by DNCB in mice.[2]
Animal Research Animal Models 6-week-old female Balb/c mice
Dosages 100 μg, 200 μg, 500 μg
Administration Topical
NCT Number Recruitment Conditions Sponsor/Collaborators Start Date Phases
NCT05617833 Not yet recruiting
Intraventricular Hemorrhage of Prematurity
Johns Hopkins University|Eunice Kennedy Shriver National Institute of Child Health and Human Development (NICHD)
 January 31 2024 Phase 1
NCT05827575 Recruiting
Functional Dyspepsia|Sleep Disturbances
Second Affiliated Hospital of Xi''an Jiaotong University
 March 30 2022 Not Applicable
NCT02537431 Completed
X-linked Hypophosphatemia
Kyowa Kirin Inc.|Kyowa Kirin Co. Ltd.
 December 23 2015 Phase 3

Chemical Information & Solubility

Molecular Weight 2846.46 Formula

C131H229N39O31
CAS No. 20449-79-0, 37231-28-0 SDF --
Smiles CCC(C)C(NC(=O)CN)C(=O)NCC(=O)NC(C)C(=O)NC(C(C)C)C(=O)NC(CC(C)C)C(=O)NC(CCCCN)C(=O)NC(C(C)C)C(=O)NC(CC(C)C)C(=O)NC(C(C)O)C(=O)NC(C(C)O)C(=O)NCC(=O)NC(CC(C)C)C(=O)N1CCCC1C(=O)NC(C)C(=O)NC(CC(C)C)C(=O)NC(C(C)CC)C(=O)NC(CO)C(=O)NC(CC2=C[NH]C3=C2C=CC=C3)C(=O)C(=O)C(NC(=O)C(CCCCN)NNC(CCCNC(N)=N)C(=O)NC(CCCCN)C(=O)NC(CCCNC(N)=N)C(=O)NC(CCC(N)=O)C(=O)NC(CCC(N)=O)C(N)=O)C(C)CC
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

Water : 100 mg/mL


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In vivo
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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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