Melittin

Catalog No.S9794 Synonyms: MLT, Forapin, Forapine

For research use only.

Melittin (MLT, Forapin, Forapine) is an activator of phospholipase A2 (PLA2) that stimulates the activity of the low molecular weight PLA2, while it does not the increase the activity of the high molecular weight enzyme.

Melittin Chemical Structure

CAS No. 20449-79-0, 37231-28-0

Purity & Quality Control

Choose Selective Phospholipase (e.g. PLA) Inhibitors

Other Phospholipase (e.g. PLA) Products

Biological Activity

Description Melittin (MLT, Forapin, Forapine) is an activator of phospholipase A2 (PLA2) that stimulates the activity of the low molecular weight PLA2, while it does not the increase the activity of the high molecular weight enzyme.
Targets
PLA2 [1]
In vitro

In vitro studies using TNF-α/IFN-γ-stimulated human keratinocytes shows that melittin inhibits the increased expression of chemokines, such as CCL17 and CCL22, and pro-inflammatory cytokines, including IL-1β, IL-6 and IFN-γ, through the blockade of the NF‐κB and STAT signalling pathways.[2]

In vivo

Melittin exhibits potent anti-atopic activities, shown by decreased Atopic dermatitis (AD)-like skin lesions, induced by DNCB in mice.[2]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: HaCaT cell line
  • Concentrations: 0.1 μg/mL, 0.5 μg/mL, 1 μg/mL
  • Incubation Time: 30 min
  • Method:

    The human keratinocyte HaCaT cell line is cultured in high glucose DMEM supplemented with 10% (v/v) FBS and antibiotics at 37°C in a humidified 5% CO2 incubator. HaCaT (5.0 × 105 cells/mL) cells are seeded in the complete medium. 24 hours later, the medium is changed to serumfree mediumwith bee venom (1, 10 and 100 ng/mL) and melittin (0.1, 0.5 and 1 μg/mL). After 30 min, the cells are stimulated with 10 ng/mL TNF-α/IFN-γ for 9 h.

Animal Research:

[2]

  • Animal Models: 6-week-old female Balb/c mice
  • Dosages: 100 μg, 200 μg, 500 μg
  • Administration: Topical

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 2846.46
Formula

C131H229N39O31

CAS No. 20449-79-0, 37231-28-0
Storage 3 years -20°C powder
2 years -80°C in solvent

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02537431 Completed Biological: burosumab X-linked Hypophosphatemia Ultragenyx Pharmaceutical Inc|Kyowa Kirin Co. Ltd. December 23 2015 Phase 3
NCT02304367 Completed Biological: Burosumab Tumor Induced Osteomalacia (TIO)|Epidermal Nevus Syndrome (ENS) Ultragenyx Pharmaceutical Inc March 24 2015 Phase 2
NCT02363946 Terminated Drug: ARC-AAT Injection|Other: Placebo|Drug: Diphenhydramine Alpha-1 Antitrypsin Deficiency Arrowhead Pharmaceuticals February 2015 Phase 1

(data from https://clinicaltrials.gov, updated on 2022-08-01)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy Melittin | Melittin supplier | purchase Melittin | Melittin cost | Melittin manufacturer | order Melittin | Melittin distributor