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Mebendazole Parasite inhibitor

Name: Mebendazole
Brand: Selleck Chemicals
SKU: S4610
Availability: InStock
Rating: 5 (7 reviews)

Catalog No.S4610 Purity:99.99%

Mebendazole is a synthetic benzimidazole derivate and anthelmintic agent. This compound interferes with the reproduction and survival of helminths by inhibiting the formation of their cytoplasmic microtubules, thereby selectively and irreversibly blocking glucose uptake.

Chemical Structure

Molecular Weight: 295.29

Purity & Quality Control

Batch: Purity: 99.99%

99.99

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Mechanism of Action

In vitro
In vitro	mebendazole (MZ), a derivative of benzimidazole, induces a dose- and time-dependent apoptotic response in human lung cancer cell lines. This compound arrests cells at the G2-M phase before the onset of apoptosis. Its treatment also results in mitochondrial cytochrome c release, followed by apoptotic cell death. Additionally, this chemical appears to be a potent inhibitor of tumor cell growth with little toxicity to normal WI38 and human umbilical vein endothelial cells^[2].
Cell Research	Cell lines	A549, WI38 normal fibroblasts, H1299 and H460 cancer cell line
	Concentrations	0, 0.2, 0.5, 1 μM
	Incubation Time	48 h
	Method	When grown to 40–50% confluence, the cells are exposed to Mebendazole dissolved in DMSO. Cell growth is monitored by counting the viable cells using a hemacytometer.

In Vivo
In vivo	When administered p.o. to nu/nu mice, Mebendazole strongly inhibits the growth of human tumor xenografts and significantly reduces the number and size of tumors in an experimental model of lung metastasis. This compound treatment significantly reduces vessel densities in mice compared with those in control mice^[2].
Animal Research	Animal Models	Mice
	Dosages	12.5 and 25 mg/kg/day
	Administration	oral administration

References

https://pubmed.ncbi.nlm.nih.gov/25773183/
https://pubmed.ncbi.nlm.nih.gov/12231542/

Chemical Information

Molecular Weight	295.29	Formula	C₁₆H₁₃N₃O₃
CAS No.	31431-39-7	SDF	Download SDF
Synonyms	Mebenvet
Smiles	COC(=O)NC1=NC2=C(N1)C=C(C=C2)C(=O)C3=CC=CC=C3

Storage and Stability

Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
			1 month-20°Cin solvent

In vitro
Batch:

DMSO : 8 mg/mL ( (27.09 mM) Warmed with 50°C water bath; Ultrasonicated; Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.260mg/ml (0.88mM)	Taking the 1 mL working solution as an example, add 50 μL of 5.2 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.260mg/ml (0.88mM)	Taking the 1 mL working solution as an example, add 50 μL of 5.2 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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