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Eflornithine (DFMO) Hydrochloride Hydrate Ornithine Decarboxylase Inhibitor

Name: Eflornithine (DFMO) Hydrochloride Hydrate
Brand: Selleck Chemicals
SKU: S4582
Availability: InStock
Rating: 5 (9 reviews)

Cat.No.S4582

Eflornithine (DFMO) hydrochloride hydrate inhibits polyamine biosynthesis by the selective, irreversible inhibition of ornithine decarboxylase (ODC). A chemoprotective agent that blocks angiogenesis. Its biological half-life is 8 hours.

Chemical Structure

Molecular Weight: 236.64

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Quality Control

Batch: Purity: >97%

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS/MSDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS/MSDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS/MSDS

Cited in Nature Medicine for its top-tier quality
COA
Datasheet
SDS/MSDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS/MSDS

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other Decarboxylase Inhibitors	Benserazide HCl Ivospemin Maleic acid TES-1025 4-Bromo-3-hydroxybenzoic acid Chelidamic acid hydrate

Cell Culture, Treatment & Working Concentration

Cell Lines	Assay Type	Incubation Time	Activity Description	PMID
J774.1	Antitrypanosomal assay	48 hrs	Antitrypanosomal activity against Trypanosoma brucei brucei TC221 infected in mouse J774.1 cells after 48 hrs by alamar blue assay, IC50 = 22.9 μM.	22376072
A673	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells	29435139
NB1643	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells	29435139
SK-N-MC	qHTS assay		qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells	29435139
Click to View More Cell Line Experimental Data

Solubility

In vitro
Batch:

Water : 47 mg/mL

DMSO : Insoluble
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	236.64	Formula	C₆H₁₂F₂N₂O₂.HCl.H₂O	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	96020-91-6	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Difluoromethylornithine hydrochloride hydrate, MDL-71782 hydrochloride hydrate, RMI-71782 hydrochloride hydrate, α-difluoromethylornithine hydrochloride hydrate			SMILES	C(CC(C(F)F)(C(=O)O)N)CN.O.Cl

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

Targets/IC₅₀/Ki	Orn decarboxylase
In vitro	When cultured cells are treated with α-difluoromethyl-Orn, an inhibitor of polyamine biosynthesis, production of hydrogen peroxide is suppressed and programmed cell death did not occur.
In vivo	Eflornithine alters changes in vascular responsiveness associated with coarctation hypertension
References	[1] https://pubmed.ncbi.nlm.nih.gov/16844838/ [2] https://pubmed.ncbi.nlm.nih.gov/12913153/ [3] https://pubmed.ncbi.nlm.nih.gov/9107438/

Applications

Methods	Biomarkers	PMID
Western blot	c-Myc / α-tubulin	25248858
Growth inhibition assay	2T1 cells	21093499
IHC	H&E staining of pancreas p21 / PCNA	25248858
IHC/IF	ODC expression in pancreatic tumors Cav-1 / β-catenin	25248858

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2026-09-03)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT06465199	RECRUITING	Atypical Teratoid/Rhabdoid Tumor; Embryonal Tumor With Multilayered Rosettes; Ewing Sarcoma; Diffuse Intrinsic Pontine Glioma; Osteosarcoma; Neuroblastoma; DIPG Brain Tumor	Milton S. Hershey Medical Center	2026-05-13	PHASE1; PHASE2
NCT07321912	RECRUITING	Osteosarcoma; Ewing Sarcoma	Milton S. Hershey Medical Center	2026-06-05	PHASE2
NCT07468136	RECRUITING	Anaplastic Oligodendroglioma; Astrocytoma, IDH-Mutant, Grade 3; Astrocytoma, IDH-Mutant, Grade 4; Diffuse Astrocytoma; Glioblastoma, IDH-Wildtype; Malignant Glioma	Mayo Clinic	2026-06-23	PHASE1; PHASE2
NCT02559778	RECRUITING	Neuroblastoma	Giselle Sholler	2015-09	PHASE2
NCT06892678	RECRUITING	Osteosarcoma Recurrent; Ewing's Tumor Recurrent	Montefiore Medical Center	2025-04-07	PHASE1; PHASE2
NCT04301843	ACTIVE_NOT_RECRUITING	Neuroblastoma	Giselle Sholler	2020-09-25	PHASE2

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