Eflornithine (MDL-71782) hydrochloride hydrate

Catalog No.S4582 Synonyms: Difluoromethylornithine hydrochloride hydrate, DFMO hydrochloride hydrate, RMI-71782 hydrochloride hydrate, α-difluoromethylornithine hydrochloride hydrate

For research use only.

Eflornithine (Difluoromethylornithine, DFMO, MDL-71782, RMI-71782, α-difluoromethylornithine) hydrochloride hydrate inhibits polyamine biosynthesis by the selective, irreversible inhibition of ornithine decarboxylase (ODC). A chemoprotective agent that blocks angiogenesis. Its biological half-life is 8 hours.

Eflornithine (MDL-71782) hydrochloride hydrate Chemical Structure

CAS No. 96020-91-6

Selleck's Eflornithine (MDL-71782) hydrochloride hydrate has been cited by 4 Publications

Purity & Quality Control

Choose Selective Decarboxylase Inhibitors

Biological Activity

Description Eflornithine (Difluoromethylornithine, DFMO, MDL-71782, RMI-71782, α-difluoromethylornithine) hydrochloride hydrate inhibits polyamine biosynthesis by the selective, irreversible inhibition of ornithine decarboxylase (ODC). A chemoprotective agent that blocks angiogenesis. Its biological half-life is 8 hours.
Targets
Orn decarboxylase [2]
In vitro

When cultured cells are treated with α-difluoromethyl-Orn, an inhibitor of polyamine biosynthesis, production of hydrogen peroxide is suppressed and programmed cell death did not occur[1].

Cell Data
Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID
J774.1 MnnNRY51cXS{eYDhco9{d22jbDDhd5NigQ>? MmfsOFghcHK| MmTKRY51cXS{eYDhco9{d22jbDDhZ5Rqfmm2eTDh[4FqdnO2IGTyfZBidm:|b33hJIJzfWOnaTDidpVk\WliVFOyNlEhcW6oZXP0[YQhcW5ibX;1d4UhUjd5ND6xJINmdGy|IHHmeIVzKDR6IHjyd{BjgSCjbHHtZZIh[my3ZTDhd5NigSxiSVO1NEA:KDJ{Lkmg{txONg>? NWrINo46RGFidHHy[4V1RSehYnzhcosoKGi{ZX[9K4h1fHC|Oj:vdJVjdWWmLn7jZokvdmyvLn7pbE5od3ZxMkKzO|YxPzJpPkKyN|c3ODd{PD;hQi=>
A673 M2XnbZFJXFNiYYPzZZk> NH[3UFhyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiQU[3N{Bk\Wyucx?= NFr2UXg9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
NB1643 NUj6bnZieUiWUzDhd5NigQ>? NFvsS4FyUFSVIH;mJJBm\GmjdILpZ{Bk[W6lZYKgZ4VtdCCuaX7ld{B1dyCrZHXueIlngSCvdXz0bZBt\SCxcIDvdpR2dmm2aXXzJIZweiCmcoXnJJJmeHW{cH;zbY5oQiCScnntZZJ6KHOlcnXlckBnd3JiTlKxOlQ{KGOnbHzz MVO8ZUB1[XKpZYS9K39jdGGwazegbJJm\j1paIT0dJM7Ny:ydXLt[YQvdmOkaT7ucI0vdmmqLnfvek8zQTR|NUGzPUc,Ojl2M{WxN|k9N2F-
SK-N-MC MofydWhVWyCjc4PhfS=> MVHxTHRUKG:oIIDl[IlifHKrYzDjZY5k\XJiY3XscEBtcW6nczD0c{Bq\GWwdHnmfUBufWy2aYDs[UBweHCxcoT1col1cWW|IH\vdkBlenWpIILldJVzeG:|aX7nPkBRemmvYYL5JJNkemWnbjDmc5IhW0tvTj3NR{Bk\Wyucx?= NEHYfJI9[SC2YYLn[ZQ:L1:kbHHub{chcHKnZk2nbJR1eHN8Lz;weYJu\WRwbnPibU5vdG1wbnnoModwfi9{OUSzOVE{QSd-Mkm0N|UyOzl:L3G+
Assay
Methods Test Index PMID
Western blot c-Myc / α-tubulin 25248858
Growth inhibition assay 2T1 cells 21093499
IHC H&E staining of pancreas ; p21 / PCNA 25248858
IHC/IF ODC expression in pancreatic tumors ; Cav-1 / β-catenin 25248858

Protocol (from reference)

Cell Research:

[1]

  • Cell lines: BY2 cell
  • Concentrations: 2 mM
  • Incubation Time: 48 h
  • Method:

    BY2 cells are treated with or without cryptogein in the presence or absence of DFMO(Difluoromethylornithine) and monitered the effect of chemicals on cell growth.

Solubility (25°C)

In vitro

Water 47 mg/mL
(198.61 mM)
DMSO Insoluble
Ethanol Insoluble

Chemical Information

Molecular Weight 236.64
Formula

C6H12F2N2O2.HCl.H2O

CAS No. 96020-91-6
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C(CC(C(F)F)(C(=O)O)N)CN.O.Cl

In vivo Formulation Calculator (Clear solution)

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Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Molarity Calculator

Mass Concentration Volume Molecular Weight

Clinical Trial Information

NCT Number Recruitment Interventions Conditions Sponsor/Collaborators Start Date Phases
NCT04696029 Recruiting Drug: Difluoromethylornithine Medulloblastoma Giselle SaulnierSholler|Atrium Health March 29 2021 Phase 2
NCT03536728 Recruiting Drug: AMXT1501|Drug: DFMO Cancer|Solid Tumor|Solid Carcinoma|Advanced Cancer Aminex Therapeutics Inc. June 12 2018 Phase 1
NCT02794428 Recruiting Drug: Eflornithine|Other: Eflornithine placebo Gastric Cancer|Gastric Intestinal Metaplasia Vanderbilt-Ingram Cancer Center|National Cancer Institute (NCI)|Cancer Prevention Pharmaceuticals Inc. September 19 2016 Phase 2
NCT02636569 Active not recruiting Drug: topical diclofenac daily|Drug: placebo Non-melanoma Skin Cancer University of Alabama at Birmingham February 2016 Not Applicable
NCT01636128 Withdrawn Drug: difluoromethylornithine|Drug: Sulindac Focus of Study: Drug Response Biomarkers Chemoprevention Neoplasms Cancer Prevention Pharmaceuticals Inc.|University of Arizona March 2014 Phase 2

(data from https://clinicaltrials.gov, updated on 2022-01-17)

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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