DNPH1i(N6-benzyladenosine)

Synonyms: N6-benzyladenosine, BAPR

DNPH1i (N6-benzyladenosine,BAPR) is a competitive inhibitor of adenosine deaminase(ADA) from L-1210 cells in axenic culture as well as a potent antiproliferative agent in vitro and in vivo.

DNPH1i(N6-benzyladenosine) Chemical Structure

DNPH1i(N6-benzyladenosine) Chemical Structure

CAS: 4294-16-0

Purity & Quality Control

Batch: Purity: 99.90%
99.90

DNPH1i(N6-benzyladenosine) Related Products

Choose Selective Adenosine Deaminase Inhibitors

Biological Activity

Description DNPH1i (N6-benzyladenosine,BAPR) is a competitive inhibitor of adenosine deaminase(ADA) from L-1210 cells in axenic culture as well as a potent antiproliferative agent in vitro and in vivo.
In vitro
In vitro

DNPH1i is the DNPH1 competitive inhibitor, that co-treatmented with hmdU selectively kills the HR-deficient cells, BRCA1-defective cells and PARPi-resistant BRCA1-mutant cell lines depleted of PARP1, sensitizing BRCA-deficient cells to PARPi or hmdU treatment.[2]

Cell Research Cell lines SUM149 BRCA1mut (parental) and WT (revertant) cell lines
Concentrations 0.3 µM
Incubation Time 8 days
Method

SUM149 BRCA1mut (parental) and WT (revertant) cell lines are either untreated or treated with hmdU in the absence or presence of DNPH1i (0.3 μM) for 8 days. Viability is determined using CellTiter-Glo.

Chemical Information & Solubility

Molecular Weight 357.36 Formula

C17H19N5O4

CAS No. 4294-16-0 SDF --
Smiles OCC1OC(C(O)C1O)[N]2C=NC3=C(NCC4=CC=CC=C4)N=CN=C23
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 71 mg/mL ( (198.67 mM); Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 1 mg/mL

Water : Insoluble


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

* Indicates a Required Field

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.
Tags: buy DNPH1i(N6-benzyladenosine) | DNPH1i(N6-benzyladenosine) supplier | purchase DNPH1i(N6-benzyladenosine) | DNPH1i(N6-benzyladenosine) cost | DNPH1i(N6-benzyladenosine) manufacturer | order DNPH1i(N6-benzyladenosine) | DNPH1i(N6-benzyladenosine) distributor