Benzyl isothiocyanate

For research use only.

Catalog No.S4783 Synonyms: Benzoylthiocarbimide, Isothiocyanic Acid Benzoyl Ester

1 publication

Benzyl isothiocyanate Chemical Structure

CAS No. 622-78-6

Benzyl isothiocyanate (BITC, Benzoylthiocarbimide, Isothiocyanic Acid Benzoyl Ester) is an isothiocyanate originally found in cruciferous vegetables that exhibits immunomodulatory, anti-parasitic, antibiotic, antioxidative, anti-atherosclerotic, anti-angiogenic, anti-metastatic, anticancer chemotherapeutic, and chemopreventive activities.

Selleck's Benzyl isothiocyanate has been cited by 1 publication

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Biological Activity

Description Benzyl isothiocyanate (BITC, Benzoylthiocarbimide, Isothiocyanic Acid Benzoyl Ester) is an isothiocyanate originally found in cruciferous vegetables that exhibits immunomodulatory, anti-parasitic, antibiotic, antioxidative, anti-atherosclerotic, anti-angiogenic, anti-metastatic, anticancer chemotherapeutic, and chemopreventive activities.
In vitro

Benzyl isothiocyanate (BITC) is highly effective in suppressing cancer initiation by modulating carcinogen-activating (phase I) and -detoxifying (phase II) enzymes during initiation events of tumor growth. It affects cell-cycle regulation, induction of apoptotic and autophagy events, tumor invasion and metastasis, angiogenesis, and EMT, and it can eliminate CSCs[1]. BITC treatment would cause permanent damage to the cells[2].

In vivo BITC inhibits chemically induced cancer, oncogenic-driven tumor formation, and human tumor xenografts in rodent cancer models. It decreases pulmonary metastasis multiplicity and volume of 4T1 murine mammary carcinoma cells injected into the inguinal mammary fat pads of syngeneic female BALB/c mice. Pharmacokinetic studies suggest that low μmol/L concentrations of BITC are sufficient for tumor inhibitory activities. BITC accumulates rapidly in cancer cells and is conjugated with intracellular thiols, particularly glutathione (GSH) and cysteine[1]. BITC is an inhibitor of B[a]P-induced lung tumorigenesis in A/J mice[3].

Protocol

Cell Research:[2]
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  • Cell lines: Capan-2 cells
  • Concentrations: 0-40 μmol/L
  • Incubation Time: 12, 24, 48, or 72 h
  • Method: The cells are treated with BITC for 12, 24, 48, or 72 h. Effect of BITC on proliferation of Capan-2 or acinar and ductal cells is determined by Sulforhodamine B assay. The plates are read at 570 nm with a Bio Kinetics plate reader.
    (Only for Reference)
Animal Research:[3]
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  • Animal Models: Female A/J mice
  • Dosages: --
  • Administration: by gavage
    (Only for Reference)

Solubility (25°C)

In vitro DMSO 29 mg/mL (194.35 mM)

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 149.21
Formula

C8H7NS

CAS No. 622-78-6
Storage powder
in solvent
Synonyms Benzoylthiocarbimide, Isothiocyanic Acid Benzoyl Ester
Smiles C1=CC=C(C=C1)CN=C=S

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Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID