Benzyl isothiocyanate

Catalog No.S4783 Synonyms: Benzoylthiocarbimide, Isothiocyanic Acid Benzoyl Ester

For research use only.

Benzyl isothiocyanate (BITC, Benzoylthiocarbimide, Isothiocyanic Acid Benzoyl Ester) is an isothiocyanate originally found in cruciferous vegetables that exhibits immunomodulatory, anti-parasitic, antibiotic, antioxidative, anti-atherosclerotic, anti-angiogenic, anti-metastatic, anticancer chemotherapeutic, and chemopreventive activities.

Benzyl isothiocyanate Chemical Structure

CAS No. 622-78-6

Selleck's Benzyl isothiocyanate has been cited by 1 Publication

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Choose Selective Antibiotics Inhibitors

Biological Activity

Description Benzyl isothiocyanate (BITC, Benzoylthiocarbimide, Isothiocyanic Acid Benzoyl Ester) is an isothiocyanate originally found in cruciferous vegetables that exhibits immunomodulatory, anti-parasitic, antibiotic, antioxidative, anti-atherosclerotic, anti-angiogenic, anti-metastatic, anticancer chemotherapeutic, and chemopreventive activities.
In vitro

Benzyl isothiocyanate (BITC) is highly effective in suppressing cancer initiation by modulating carcinogen-activating (phase I) and -detoxifying (phase II) enzymes during initiation events of tumor growth. It affects cell-cycle regulation, induction of apoptotic and autophagy events, tumor invasion and metastasis, angiogenesis, and EMT, and it can eliminate CSCs[1]. BITC treatment would cause permanent damage to the cells[2].

In vivo BITC inhibits chemically induced cancer, oncogenic-driven tumor formation, and human tumor xenografts in rodent cancer models. It decreases pulmonary metastasis multiplicity and volume of 4T1 murine mammary carcinoma cells injected into the inguinal mammary fat pads of syngeneic female BALB/c mice. Pharmacokinetic studies suggest that low μmol/L concentrations of BITC are sufficient for tumor inhibitory activities. BITC accumulates rapidly in cancer cells and is conjugated with intracellular thiols, particularly glutathione (GSH) and cysteine[1]. BITC is an inhibitor of B[a]P-induced lung tumorigenesis in A/J mice[3].

Protocol (from reference)

Cell Research:[2]
  • Cell lines: Capan-2 cells
  • Concentrations: 0-40 μmol/L
  • Incubation Time: 12, 24, 48, or 72 h
  • Method: The cells are treated with BITC for 12, 24, 48, or 72 h. Effect of BITC on proliferation of Capan-2 or acinar and ductal cells is determined by Sulforhodamine B assay. The plates are read at 570 nm with a Bio Kinetics plate reader.
Animal Research:[3]
  • Animal Models: Female A/J mice
  • Dosages: --
  • Administration: by gavage

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 149.21
Formula

C8H7NS

CAS No. 622-78-6
Storage 2 years -20°C liquid
Smiles C1=CC=C(C=C1)CN=C=S

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