Home Microbiology Antibiotics inhibitor Benzyl isothiocyanate

research use only

Benzyl isothiocyanate Antibiotics inhibitor

Cat.No.S4783

Benzyl isothiocyanate (BITC, Benzoylthiocarbimide, Isothiocyanic Acid Benzoyl Ester) is an isothiocyanate originally found in cruciferous vegetables that exhibits immunomodulatory, anti-parasitic, antibiotic, antioxidative, anti-atherosclerotic, anti-angiogenic, anti-metastatic, anticancer chemotherapeutic, and chemopreventive activities.
Benzyl isothiocyanate Antibiotics inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 149.21

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: S478301 DMSO]29 mg/mL]false]]]false]]]false Purity: 99.58%
99.58

Chemical Information, Storage & Stability

Molecular Weight 149.21 Formula

C8H7NS

 Storage (From the date of receipt) 2 years -20°C liquid
CAS No. 622-78-6 Download SDF Storage of Stock Solutions

Synonyms Benzoylthiocarbimide, Isothiocyanic Acid Benzoyl Ester Smiles C1=CC=C(C=C1)CN=C=S

Solubility

In vitro
Batch:

DMSO : 29 mg/mL ( (194.35 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo
Batch:

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

In vitro
Benzyl isothiocyanate (BITC) is highly effective in suppressing cancer initiation by modulating carcinogen-activating (phase I) and -detoxifying (phase II) enzymes during initiation events of tumor growth. It affects cell-cycle regulation, induction of apoptotic and autophagy events, tumor invasion and metastasis, angiogenesis, and EMT, and it can eliminate CSCs[1]. BITC treatment would cause permanent damage to the cells[2].
In vivo
BITC inhibits chemically induced cancer, oncogenic-driven tumor formation, and human tumor xenografts in rodent cancer models. It decreases pulmonary metastasis multiplicity and volume of 4T1 murine mammary carcinoma cells injected into the inguinal mammary fat pads of syngeneic female BALB/c mice. Pharmacokinetic studies suggest that low μmol/L concentrations of BITC are sufficient for tumor inhibitory activities. BITC accumulates rapidly in cancer cells and is conjugated with intracellular thiols, particularly glutathione (GSH) and cysteine[1]. BITC is an inhibitor of B[a]P-induced lung tumorigenesis in A/J mice[3].
References

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

Please enter your name.
Please enter your email. Please enter a valid email address.
Please write something to us.