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ART558 Polθ inhibitor

Name: ART558
Brand: Selleck Chemicals
SKU: S9936
Availability: InStock
Rating: 5 (6 reviews)

Cat.No.S9936

ART558 is a potent, selective, low molecular weight, allosteric inhibitor of DNA Polθ with an IC50 of 7.9 nM.

Chemical Structure

Molecular Weight: 418.41

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Quality Control

Batch: S993601 DMSO]84 mg/mL]false]Ethanol]21 mg/mL]false]Water]Insoluble]false Purity: 99.76%

Cited in Nature Medicine for its top-tier quality
COA
NMR
HPLC
SDS/MSDS
Datasheet

99.76

Related Targets	HDAC PARP ATM/ATR DNA-PK WRN DNA/RNA Synthesis Topoisomerase PPAR Sirtuin Casein Kinase
Other Polθ Inhibitors	ART899 RP-6685 ART812 PolQi2

Solubility in DMSO or Water

In vitro
Batch:

DMSO : 84 mg/mL (200.76 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 21 mg/mL

Water : Insoluble

The solubility data above are all experimental results (not literature values).

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Search directly for a cell line, or select a cell line to find matched working concentrations

Mechanism of Action

Information	ART558 is a potent, specific DNA polymerase theta (Pol θ) inhibitor. It affects DNA damage repair signaling by increasing Pol θ residence time at damage sites and blocking theta-mediated end joining, effectively radiosensitizing tumor cells and promoting apoptosis in BRCA-deficient cells.
Read more about ART558 IC50 for cell-based and cell-free assays
Primary Applications and Mechanism of Action

Chemical Information, Storage & Stability

Molecular Weight	418.41	Formula	C₂₁H₂₁F₃N₄O₂	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	2603528-97-6	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

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