Name: Ampicillin sodium
Brand: Selleck Chemicals
SKU: S3170
Availability: InStock
Rating: 5 (4 reviews)

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Quality Control

Batch: Purity: >97%

Cited in Nature Medicine for its top-tier quality
COA
Datasheet
SDS/MSDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS/MSDS

Cited in Nature Medicine for its top-tier quality
COA
NMR
Datasheet
SDS/MSDS

Cited in Nature Medicine for its top-tier quality
COA
Datasheet
SDS/MSDS

97

Related Targets	Integrase Bacterial Antibiotics Anti-infection Fungal Antiviral COVID-19 Parasite Reverse Transcriptase HIV
Other Selection Antibiotics for Transfected Cell Inhibitors	Blasticidin S Hydrochloride Ribostamycin Sulfate D-Cycloserine

Solubility

In vitro
Batch:

DMSO : 74 mg/mL (199.25 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 74 mg/mL

Ethanol : 74 mg/mL

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Clear solution	5% DMSO 1 95% Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	0.740mg/ml (1.99mM)	Taking the 1 mL working solution as an example, add 50 μL of 14.8 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	3.700mg/ml (9.96mM)	Taking the 1 mL working solution as an example, add 50 μL of 74 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	371.39	Formula	C₁₆H₁₈N₃NaO₄S	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	69-52-3	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	D-(-)-α-Aminobenzylpenicillin sodium salt			SMILES	CC1(C(N2C(S1)C(C2=O)NC(=O)C(C3=CC=CC=C3)N)C(=O)[O-])C.[Na+]

Read more about storage stability stock solution CAS number SMILES

Mechanism of Action

Targets/IC₅₀/Ki	beta-lactamase
In vitro	Belonging to the penicillin group of beta-lactam antibiotics, ampicillin is able to penetrate Gram-positive and some Gram-negative bacteria. Ampicillin acts as a competitive inhibitor of the enzyme transpeptidase, which is needed by bacteria to make their cell walls. Ampicillin inhibits the third and final stage of bacterial cell wall synthesis in binary fission, which ultimately leads to cell lysis.
References	[1] https://pubmed.ncbi.nlm.nih.gov/358200/ [2] https://pubmed.ncbi.nlm.nih.gov/111546/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2025-12-18)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT07183462	RECRUITING	Pregnancy Complications, Infectious; Premature Birth; Neonatal Diseases and Abnormalities; Antibiotic Prophylaxis; Latency Period; Prematurity	Sheba Medical Center	2025-09-10	PHASE4
NCT05977400	NOT_YET_RECRUITING	Premature Birth; Antibiotics; Neurodevelopment	Sharp HealthCare	2024-04	PHASE3
NCT06406114	RECRUITING	Drug Allergy; Cephalosporin Allergy; Drug Hypersensitivity; Antibiotic Allergy; Beta Lactam Adverse Reaction; Drug-Induced Anaphylaxis; Cephalosporin Reaction	Massachusetts General Hospital	2025-05-05	PHASE2
NCT06494072	RECRUITING	Community Acquired Pneumonia in Children	Medical College of Wisconsin	2024-08-22	PHASE4
NCT07326540	RECRUITING	Hospital Acquired Bacterial Pneumonia (HABP); Ventilator Associated Bacterial Pneumonia (VABP); Colistin Resistanrt ABC; Acinetobacter Baumannii-calcoaceticus Complex	BioVersys SAS	2026-03-27	PHASE3
NCT06423898	RECRUITING	Endocarditis, Infective	Fundación Pública Andaluza para la gestión de la Investigación en Sevilla	2024-10-09	PHASE4

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Handling Instructions

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Ampicillin sodium Selection Antibiotics for Transfected Cell inhibitor

Chemical Structure

Molecular Weight: 371.39