CAS No. 69-52-3

Batch: Purity:97
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Ampicillin sodium Chemical Structure
Chemical Structure
Check the Ampicillin sodium product page for in-depth specifications, including solubility, stock solutions, MOA, and working concentrations.

Chemical Information

IUPAC Name sodium (2S,5R,6R)-6-[[(2R)-2-amino-2-phenylacetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylate
CAS Number 69-52-3
Molecular Formula

C16H18N3NaO4S
Molecular Weight (MW) 371.39
SMILES CC1(C(N2C(S1)C(C2=O)NC(=O)C(C3=CC=CC=C3)N)C(=O)[O-])C.[Na+]
InChIKey KLOHDWPABZXLGI-YWUHCJSESA-M

Biological Activity

Ampicillin sodium is a broad-spectrum beta-lactam antibiotic that targets penicillin-binding proteins (PBPs). Inhibition of PBPs disrupts bacterial cell wall peptidoglycan synthesis by blocking the transpeptidation step, which prevents essential cross-linking within the bacterial cell wall. This mechanism leads to cell wall destabilization, osmotic lysis, and bacterial cell death, demonstrating broad antibacterial activity against both Gram-positive and Gram-negative bacteria.

How to Prepare Stock Solution?

Concentration Volume Mass
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Solubility

Batch:
All in vitro solubility values are batch-tested experimental data (non-literature values).
Solvent Solubility & Note
DMSO 74 mg/mL (199.25 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO. The recommended stock concentration is 10 mM in DMSO.)
Water 74 mg/mL
Ethanol 74 mg/mL
FAQ
Q: Why does a DMSO stock precipitate in media/saline?
A: It is caused by unheated aqueous media (supersaturation), local solvent shock, or incorrect mixing sequence.
How to fix it?
  • Preheat media/saline to 37°C.
  • Mix rapidly (pipette/vortex) upon addition.
  • For complex mixes, always add the aqueous phase last.
  • Re-dissolve minor precipitates with a 37°C water bath and sonication (≤30 min).

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Note: Technical data last updated: Sep 1, 2026.