Name: Z-IETD-FMK
Brand: Selleck Chemicals
SKU: S7314
Availability: InStock
Rating: 5 (77 reviews)

Chemical Structure

Molecular Weight: 654.68

Purity & Quality Control

Batch: Purity: 99.88%

99.88

Products Often Used Together with Z-IETD-FMK

Z-LEHD-FMK TFA

It and Z-LEHD-FMK TFA are used to assess if caspase-8 and 9 affect the pro-survival actions of melatonin under hypoxia conditions in granuloma cells.

Nimesulide

It suppresses Nimesulide and radiation-induced cleavage activation of caspase-9/3/8 and Bid in A549 cells.

Z-VAD-FMK

Pretreatment with this compound and Z-VAD-FMK significantly attenuates 23-HUA-induced DNA fragmentation in HL-60 cells.

Q-VD-Oph

In DEF cells, Q-VD-Oph effectively promotes viral replication more than this compound.

Related Products

Related Targets	Caspase-1 Caspase-3 Caspase-8 Caspase-9 Caspase-4 Capase-7 Caspase-2 Caspase-5 Caspase-6 Caspase-10 Caspase-7	Click to Expand
Related Products	Z-VAD-FMK Belnacasan (VX-765) Z-DEVD-FMK Q-VD-Oph Emricasan (IDN-6556) Ac-DEVD-CHO Z-LEHD-FMK TFA Z-VAD(OH)-FMK (Caspase Inhibitor VI) PAC-1 Z-YVAD-FMK Apoptosis Activator 2 Tasisulam Phenoxodiol (Haginin E)	Click to Expand
Related Compound Libraries	Autophagy Compound Library Apoptosis Compound Library Ferroptosis Compound Library Pyroptosis Compound Library Mitochondria-Targeted Compound Library	Click to Expand

Signaling Pathway

Caspase Signaling Pathway

Cell Culture and Working Concentration

Cell Lines	Assay Type	Concentration	Incubation Time	Activity Description	PMID
HepG2	Function assay			Z-IETD-FMK accelerates the PPI-induced cell death of HepG2 cells.	30671458
HL-60	Function assay	50 μM	1 h	completely blocks the 23-HUA-induced DNA fragmentation	30551620
MG63	Cytotoxicity assay	10 μM		partially reversed MSP-4-induced MG63 cell cytotoxicity	29301308
HXO-RB44 cells	Apoptosis assay			decreases the apoptotic rates induced by bufalin	27904697
Jurkat cells	Function assay	50 μM	1 h	abolishes the cleavage of tBid	25632401
DU 145	Function assay	20 μM	24 h	interrupted the cleavage of PARP induced by ergosterol peroxide	25506265
Click to View More Cell Line Experimental Data

Mechanism of Action

Targets

Caspase-8 ^[1]

In vitro
In vitro	Z-IETD-FMK, which inhibits the cleavage of caspase-8 and partially inhibits the cleavage of caspase-3 and PARP, prevents the execution of apoptosis in retinal cells exposed to different apoptotic stimuli. ^[1] This compound (50 μM) reduces ceramide-induced cardiomyocyte death and significantly inhibits the activation of caspase 3. ^[2] Inhibition of caspase-8 by this chemical affects the generation of activated/memory T cells and T cell cytokine production, and decreases NF-kappaB responses to TCR:CD3 engagement in T cell cultures. ^[3]
Cell Research	Cell lines	Rat neonatal cardiomyocytes
	Concentrations	50 µM
	Incubation Time	24 h
	Method	Drugs were added to the cell culture medium simultaneously with ceramide, and surviving cells were counted 24 hours later.

In Vivo
In vivo	In vivo, inhibition of caspase-8 by Z-IETD-FMK reduces memory/activated CD4 and CD8 T cells, and increases susceptibility to T. cruzi infection. ^[3] This compound promotes neuronal survival and regeneration of injured retinal ganglion cells after CNS injuries. ^[4]
Animal Research	Animal Models	T. cruzi-infected mice
	Dosages	0.4 mg/3 days
	Administration	--

References

https://pubmed.ncbi.nlm.nih.gov/10509663/
https://pubmed.ncbi.nlm.nih.gov/10997751/
https://pubmed.ncbi.nlm.nih.gov/15879131/

https://pubmed.ncbi.nlm.nih.gov/21775595/

+ Expand

Chemical Information

Molecular Weight	654.68	Formula	C₃₀H₄₃FN₄O₁₁
CAS No.	210344-98-2	SDF	Download SDF
Synonyms	Caspase-8 Inhibitor, Z-IE(OMe)TD(OMe)-FMK
Smiles	CCC(C)C(C(=O)NC(CCC(=O)OC)C(=O)NC(C(C)O)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)OCC1=CC=CC=C1

Storage and Stability

Storage (From the date of receipt)	3 years-20°Cpowder	Storage of Stock Solutions Aliquot the stock solutions to avoid repeated freeze-thaw cycles	1 year-80°Cin solvent
			1 month-20°Cin solvent

In vitro
Batch:

DMSO : 100 mg/mL ( (152.74 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molecular Weight Calculator

In vivo Batch: Add solvents to the product individually and in order.				In vivo Formulation Calculator
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (7.64mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to make it clear; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	2.250mg/ml (3.44mM)	Taking the 1 mL working solution as an example, add 50 μL of 45 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.

Preparing Stock Solutions

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
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Dilution Calculator Molecular Weight Calculator

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

Tel: +1-832-582-8158 Ext:3
If you have any other enquiries, please leave a message.

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Z-IETD-FMK Caspase inhibitor