research use only

Methacycline HCl Antibiotics inhibitor

Name: Methacycline HCl
Brand: Selleck Chemicals
SKU: S2527
Availability: InStock
Rating: 5 (10 reviews)

Cat.No.S2527

Methacycline HCl (Rondomycin) is a tetracycline antibiotic, and also an inhibitor of epithelial–mesenchymal transition (EMT) with IC50 of roughly 5 μM, used to treat various infections.

Chemical Structure

Molecular Weight: 478.88

Jump to Mechanism of Action Solubility Chemical Information, Storage & Stability

Quality Control

Batch: Purity: 99.68%

99.68

Related Targets	Bacterial Anti-infection Fungal Antiviral COVID-19 Parasite Reverse Transcriptase HIV HCV Protease SARS-CoV HIV Protease Influenza Virus β-lactamase Integrase HBV CMV Neuraminidase HCV Thioredoxin Reductase HSV RSV Enterovirus 3C-Like Protease Ribosomal Peptidyl Transferase HPV
Related Products	G418 Sulfate (Geneticin) Nanchangmycin Fusidine Sitafloxacin Hydrate Gamithromycin Tildipirosin Spiramycin Nadifloxacin 6-Aminopenicillanic acid Thiamphenicol Duocarmycin SA Cefetamet pivoxil hydrochloride Tilmicosin Succinylsulfathiazole Phleomycin D1 Sarafloxacin HCl Farnesol Sulbenicillin Sodium Orbifloxacin Cefoselis Sulfate Josamycin Kasugamycin hydrochloride hydrate Apramycin Sulfate Ormetoprim Cephalotin acid Kitasamycin KKL-35 Puromycin Tosufloxacin p-Toluenesulfonate Hydrate Fluoroquinolonic Acid

Chemical Information, Storage & Stability

Molecular Weight	478.88	Formula	C₂₂H₂₂N₂O₈.HCl	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	3963-95-9	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	Rondomycin			Smiles	CN(C)C1C2C(C3C(=C)C4=C(C(=CC=C4)O)C(=C3C(=O)C2(C(=C(C1=O)C(=O)N)O)O)O)O.Cl

Solubility

In vitro
Batch:

DMSO : 95 mg/mL (198.37 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : 1 mg/mL

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
=	×	×

Dilution Calculator Molecular Weight Calculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg Average weight of animals: g Dosing volume per animal:μL Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Mechanism of Action

Targets/IC₅₀/Ki	protein synthesis ^[1]
In vitro	Methacycline inhibits TGF-β1-induced α-smooth muscle actin, Snail1, and collagen I of primary alveolar epithelial cells. Methacycline inhibits TGF-β1-induced non-Smad pathways, including c-Jun N-terminal kinase, p38, and Akt activation, but not Smad or β-catenin transcriptional activity. Methacycline has no effect on baseline c-Jun N-terminal kinase, p38, or Akt activities or lung fibroblast responses to TGF-β1.^[1]
In vivo	Methacycline (100 mg/kg, i.p.) delivered daily beginning 10 days after intratracheal bleomycin improves survival at Day 17. Methacycline attenuates Bleomycin-induced canonical EMT markers, Snail1, Twist1, collagen I, as well as fibronectin protein and mRNA. Methacycline does not attenuate inflammatory cell accumulation or alter TGF-β1-responsive genes in alveolar macrophages. ^[1]
References	[1] https://pubmed.ncbi.nlm.nih.gov/23944988/

Tech Support

Handling Instructions

Tel: +1-832-582-8158 Ext:3

If you have any other enquiries, please leave a message.

C1=C0/X	C1:	LOG(C1):
C2=C1/X	C2:	LOG(C2):
C3=C2/X	C3:	LOG(C3):
C4=C3/X	C4:	LOG(C4):
C5=C4/X	C5:	LOG(C5):
C6=C5/X	C6:	LOG(C6):
C7=C6/X	C7:	LOG(C7):
C8=C7/X	C8:	LOG(C8):