Home Microbiology Antibiotics for Mammalian Cell Culture inhibitor - Antibiotics chemical Tylosin tartrate

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Tylosin tartrate Antibiotics for Mammalian Cell Culture inhibitor

Tylosin tartrate is a macrolide antibiotic approved for the control of mycoplasmosis in poultry.

Tylosin tartrate Antibiotics for Mammalian Cell Culture inhibitor Chemical Structure

Chemical Structure

Molecular Weight: 1066.19

Purity & Quality Control

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Mechanism of Action

In vitro
In vitro Tylosin tartrate (25 mg/L) significantly inhibits methane production relative to unamended controls, thus indicating that this compound at concentrations commonly found in swine lagoons can negatively impact anaerobic metabolism. [1] Its disappearance follows a biphasic pattern, where rapid initial loss is followed by a slow removal phase. The 90% disappearance times for tylosin, relomycin (tylosin D), and desmycosin (tylosin B) in anaerobically incubated slurries are 30 to 130 hours, and aerating the slurries reduced the 90% disappearance times to between 12 and 26 hours. This chemical is stable for at least one month when stored in Milli-Q water at pH 5.7 to 6.7 at 22 ℃. [2]

In Vivo
In vivo Tylosin increases splenocyte proliferation and splenocyte conditioned medium (CM) proliferative activity above control levels from whole splenocyte cultures in chickens. Tylosin also increases antitumor activity of splenocytes in chickens. [3] This compound (50 mg/kg) administered orally twice daily for 10 days, beginning 14 days after intranasal infection, significantly reduces the incidence and severity of macroscopical pneumonic lung lesions in neonatal piglets. [4] After this chemical administered intravenously (i.v.) at 10 mg/kg in camels, serum tylosin concentrations in the water-deprived camels are significantly higher, rate of drug elimination is slower, the volume of distribution is significantly smaller, and total body clearance is significantly slower than in the normal camels. On the other hand, serum tylosin concentrations are lower in the water deprived camels and the mean absorption time is significantly shorter in camels treat with this compound (20 mg/kg i.m.). [5]

References
  • https://pubmed.ncbi.nlm.nih.gov/15839550/
  • https://pubmed.ncbi.nlm.nih.gov/15765935/
  • https://pubmed.ncbi.nlm.nih.gov/9733118/
  • https://pubmed.ncbi.nlm.nih.gov/7134653/
  • https://pubmed.ncbi.nlm.nih.gov/8583543/

Chemical Information

Molecular Weight 1066.19 Formula

C46H77NO17.C4H6O6
CAS No. 74610-55-2 SDF Download SDF
Synonyms N/A
Smiles CC1C(C=C(C=CC(=O)C(CC(C(C(C(CC(=O)O1)O)C)OC2C(C(C(C(O2)C)OC3CC(C(C(O3)C)O)(C)O)N(C)C)O)CC=O)C)C)COC4C(C(C(C(O4)C)O)OC)OC.C(C(C(=O)O)O)(C(=O)O)O

Storage and Stability

Storage (From the date of receipt)

In vitro
Batch:

DMSO : 300 mg/mL ( (281.37 mM) Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 300 mg/mL

Water : 200 mg/mL


Molecular Weight Calculator

In vivo
Batch:

Add solvents to the product individually and in order.


In vivo Formulation Calculator

Preparing Stock Solutions

Molarity Calculator

Mass Concentration Volume Molecular Weight

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

mg/kg g μL

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO % % Tween 80 % ddH2O
%DMSO %

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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