Home Microbiology Antibiotics inhibitor Benzyl isothiocyanate For research use only.

Benzyl isothiocyanate

Synonyms: Benzoylthiocarbimide, Isothiocyanic Acid Benzoyl Ester

Benzyl isothiocyanate (BITC, Benzoylthiocarbimide, Isothiocyanic Acid Benzoyl Ester) is an isothiocyanate originally found in cruciferous vegetables that exhibits immunomodulatory, anti-parasitic, antibiotic, antioxidative, anti-atherosclerotic, anti-angiogenic, anti-metastatic, anticancer chemotherapeutic, and chemopreventive activities.

Benzyl isothiocyanate Chemical Structure

Benzyl isothiocyanate Chemical Structure

CAS: 622-78-6

Selleck's Benzyl isothiocyanate has been cited by 2 publications

Purity & Quality Control

Batch: S478301 DMSO] 29 mg/mL] false] ] ] false] ] ] false Purity: 99.58%
99.58

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Biological Activity

Description Benzyl isothiocyanate (BITC, Benzoylthiocarbimide, Isothiocyanic Acid Benzoyl Ester) is an isothiocyanate originally found in cruciferous vegetables that exhibits immunomodulatory, anti-parasitic, antibiotic, antioxidative, anti-atherosclerotic, anti-angiogenic, anti-metastatic, anticancer chemotherapeutic, and chemopreventive activities.
In vitro
In vitro Benzyl isothiocyanate (BITC) is highly effective in suppressing cancer initiation by modulating carcinogen-activating (phase I) and -detoxifying (phase II) enzymes during initiation events of tumor growth. It affects cell-cycle regulation, induction of apoptotic and autophagy events, tumor invasion and metastasis, angiogenesis, and EMT, and it can eliminate CSCs[1]. BITC treatment would cause permanent damage to the cells[2].
Cell Research Cell lines Capan-2 cells
Concentrations 0-40 μmol/L
Incubation Time 12, 24, 48, or 72 h
Method The cells are treated with BITC for 12, 24, 48, or 72 h. Effect of BITC on proliferation of Capan-2 or acinar and ductal cells is determined by Sulforhodamine B assay. The plates are read at 570 nm with a Bio Kinetics plate reader.
In Vivo
In vivo BITC inhibits chemically induced cancer, oncogenic-driven tumor formation, and human tumor xenografts in rodent cancer models. It decreases pulmonary metastasis multiplicity and volume of 4T1 murine mammary carcinoma cells injected into the inguinal mammary fat pads of syngeneic female BALB/c mice. Pharmacokinetic studies suggest that low μmol/L concentrations of BITC are sufficient for tumor inhibitory activities. BITC accumulates rapidly in cancer cells and is conjugated with intracellular thiols, particularly glutathione (GSH) and cysteine[1]. BITC is an inhibitor of B[a]P-induced lung tumorigenesis in A/J mice[3].
Animal Research Animal Models Female A/J mice
Dosages --
Administration by gavage

Chemical Information & Solubility

Molecular Weight 149.21 Formula

C8H7NS
CAS No. 622-78-6 SDF Download Benzyl isothiocyanate SDF
Smiles C1=CC=C(C=C1)CN=C=S
Storage (From the date of receipt) 2 years -20°C liquid

In vitro
Batch:

DMSO : 29 mg/mL ( (194.35 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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