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Biochanin A FAAH inhibitor

Name: Biochanin A
Brand: Selleck Chemicals
SKU: S2377
Availability: InStock
Rating: 5 (5 reviews)

Cat.No.S2377

Biochanin A (4-Methylgenistein), an O-methylated isoflavone from Trifolium pratense, inhibits protein tyrosine kinase (PTK) of epidermal growth factor receptor with IC50 values of 91.5 μM.

Chemical Structure

Molecular Weight: 284.26

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Quality Control

Batch: Purity: 99.69%

99.69

Related Targets	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Other FAAH Inhibitors	URB597 PF-3845 JNJ-42165279 PF-04457845 FAAH-IN-2 JZL195 URB937 JNJ-1661010 BIA 10-2474 LY-2183240

Solubility

In vitro
Batch:

DMSO : 56 mg/mL (197.0 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Ethanol : 16 mg/mL

Water : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

% Tween 80

% ddH₂O

% DMSO

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	284.26	Formula	C₁₆H₁₂O₅	Storage (From the date of receipt)	3 years-20°Cpowder
CAS No.	491-80-5	Download SDF		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	4-Methylgenistein			Smiles	COC1=CC=C(C=C1)C2=COC3=CC(=CC(=C3C2=O)O)O

Mechanism of Action

Targets/IC₅₀/Ki	FAAH (rat) 1.4 μM FAAH (mouse) 1.8 μM FAAH (human) 2.4 μM EGFR 91.5 μM
In vitro	Biochanin A has been reported to inhibit protein tyrosine kinase (PTK) of epidermal growth factor receptor with IC50 values of 91.5 μM This compound inhibits the hydrolysis of 0.5 μM anandamide by mouse, rat and human fatty acid amide hydrolase (FAAH) with IC50 values of 1.8, 1.4 and 2.4 μM respectively. It inhibits both serum and EGF-stimulated growth of LNCaP and DU-145 cells (IC50 values from 8.0 to 27 micrograms/ml for serum and 4.3 to 15 micrograms/ml for EGF), but has no significant effect of the EGF receptor tyrosine autophosphorylation.
In vivo	LD50: Mice 63mg/kg (i.p.)
References	[1] https://pubmed.ncbi.nlm.nih.gov/3106339/ [2] https://pubmed.ncbi.nlm.nih.gov/20590565/ [3] https://pubmed.ncbi.nlm.nih.gov/8497428/ [4] https://pubmed.ncbi.nlm.nih.gov/1844641/

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