Yoda1

Catalog No.S6678

For research use only.

Yoda1 acts as an agonist for both human and mouse Piezo1(EC50 of 17.1 and 26.6 μM for mouse and human). Yoda1 activates purified Piezo1 channels in the absence of other cellular components.

Yoda1 Chemical Structure

CAS No. 448947-81-7

Selleck's Yoda1 has been cited by 3 Publications

Purity & Quality Control

Choose Selective Mechanosensitive Channel Inhibitors

Biological Activity

Description Yoda1 acts as an agonist for both human and mouse Piezo1(EC50 of 17.1 and 26.6 μM for mouse and human). Yoda1 activates purified Piezo1 channels in the absence of other cellular components.
Targets
mPiezo1 [1]
(Cell-free assay)
hPiezo1 [1]
(Cell-free assay)
17.1 μM(EC50) 26.6 μM(EC50)
In vitro

Yoda-1 dramatically increases the YAP expression and ECM secretion reduced by Piezo1 silencing in vitro.[2]

Protocol (from reference)

Cell Research:

[2]

  • Cell lines: Immortalized mouse renal MCs (SV40-MES-13)
  • Concentrations: --
  • Incubation Time: 48 h
  • Method:

    Immortalized mouse renal MCs (SV40-MES-13) were cultured in DMEM/F12 containing 5% fetal bovine serum (FBS), 0.5% penicillin and streptomycin in an atmosphere of 5% CO2 at 37 ℃. Following overnight serum deprivation, the SV40-MES-13 cells are seeded on cover slips coated with either 2 kPa (soft) or 50 kPa (stiff) hydrogels, and then treated with the YAP inhibitor Super-TDU, Piezo1 activator Yoda-1, MEK1/2 inhibitor PD0325901, JNK inhibitor SP600125, or the p38 kinase inhibitor SB203580 for 48 h.

Solubility (25°C)

In vitro

Chemical Information

Molecular Weight 355.27
Formula

C13H8Cl2N4S2

CAS No. 448947-81-7
Storage 3 years -20°C powder
2 years -80°C in solvent
Smiles C1=CC(=C(C(=C1)Cl)CSC2=NN=C(S2)C3=NC=CN=C3)Cl

In vivo Formulation Calculator (Clear solution)

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Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Molarity Calculator

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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