Home Epigenetics HDAC inhibitor UF010 For research use only.

UF010

UF010 is a class I HDAC-selective inhibitor with IC50 values of 0.5 nM, 0.1 nM, 0.06 nM, 1.5 nM, 9.1 nM and 15.3 nM for HDAC1, HDAC2, HDAC3, HDAC8, HDAC6 and HDAC10, respectively.

UF010 Chemical Structure

UF010 Chemical Structure

CAS: 537672-41-6

Purity & Quality Control

Batch: S581001 DMSO] 54 mg/mL] false] Ethanol] 54 mg/mL] false] Water] Insoluble] false Purity: 99.58%
99.58

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Signaling Pathway

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Biological Activity

Description UF010 is a class I HDAC-selective inhibitor with IC50 values of 0.5 nM, 0.1 nM, 0.06 nM, 1.5 nM, 9.1 nM and 15.3 nM for HDAC1, HDAC2, HDAC3, HDAC8, HDAC6 and HDAC10, respectively.
Targets
HDAC3 [1]
(Cell-free assay)		 HDAC2 [1]
(Cell-free assay)		 HDAC1 [1]
(Cell-free assay)		 HDAC8 [1]
(Cell-free assay)		 HDAC6 [1]
(Cell-free assay)		 Click to View More Targets
0.06 nM 0.1 nM 0.5 nM 1.5 nM 9.1 nM
In vitro
In vitro In Cell-cycle analysis using MDA-MB-231 cells, UF010 mainly blocks G1/S transition with an increased G1 cell population and a reduced cell population in the S phase in a dose-dependent manner. UF010 has a half-life of 15.8 hr in cell culture medium containing 10% fetal bovine serum[1].
Cell Research Cell lines HCT116 cells and A549 cells
Concentrations 2 μM
Incubation Time 1 h
Method

HCT116 and A549 cells are exposed to DMSO or etoposide (Etop, 10 μM) for 6 hr. TSA (0.2 μM), MS-275, and UF010 (2 μM) are added 1 hr before cell lysis. The total cell lysates are subject to western blotting with antibodies to the indicated proteins. PCNA is detected as a loading control.

Chemical Information & Solubility

Molecular Weight 271.15 Formula

C11H15BrN2O
CAS No. 537672-41-6 SDF --
Smiles CCCCNNC(=O)C1=CC=C(C=C1)Br
Storage (From the date of receipt) 3 years -20°C powder

In vitro
Batch:

DMSO : 54 mg/mL ( (199.15 mM)； Moisture-absorbing DMSO reduces solubility. Please use fresh DMSO.)

Ethanol : 54 mg/mL

Water : Insoluble


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In vivo
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Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

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Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

Handling Instructions

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