WST-8

For research use only.

Catalog No.S7764

1 publication

WST-8 Chemical Structure

CAS No. 193149-74-5

WST-8 is a water-soluble tetrazolium salt used for assessing cell metabolic activity that produces corresponding formazan dye that absorbs at 460 nm. WST-8 is typically used as a cell viability indicator in cell proliferation assays.

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Selleck's WST-8 has been cited by 1 publication

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Biological Activity

Description WST-8 is a water-soluble tetrazolium salt used for assessing cell metabolic activity that produces corresponding formazan dye that absorbs at 460 nm. WST-8 is typically used as a cell viability indicator in cell proliferation assays.
In vitro

WST-8 is reduced by NADH in good yields at neutral pHs in the presence of 1-methoxy PMS to produce the corresponding formazan dye that absorbs at 460 nm. The formazan is soluble to water at concentrations higher than 0.1 M. WST-8 is useful as a sensitive chromogenic indicator for NADH. It is also applicable to cell proliferation assays as a cell viability indicator.[1]

Protocol

Cell Research:

[1]

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  • Cell lines: Hela cells
  • Concentrations: 0.5 mM
  • Incubation Time: 2 h
  • Method:

    A hundred μl of HeLa cell culture in the logarithmic phase is inoculated onto a 96-well microtiter plate, and the plate pre-incubated for 24 h in a CO2 incubator at 37℃. A 10 μl of the working solution containing WST-8 and lmethoxy PMS (0.5 mM and 20 μM, respectively, as the final concentration) is added to each well; it is incubated for an additional 2 h. The absorbance of each well is measured at 450 nm with a reference wavelength at 650 nm with an M-UVmax microplate reader.


    (Only for Reference)

Solubility (25°C)

In vitro Water 100 mg/mL (166.53 mM)
DMSO 4 mg/mL (6.66 mM)
Ethanol Insoluble

* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.

Chemical Information

Molecular Weight 600.47
Formula

C20H13N6NaO11S2

CAS No. 193149-74-5
Storage 3 years -20°C(in the dark) powder
2 years -80°C(in the dark) in solvent
Synonyms
Smiles COC1=C(C=CC(=C1)[N+](=O)[O-])[N+]2=NC(=NN2C3=CC=C(C=C3)[N+](=O)[O-])C4=C(C=C(C=C4)S(=O)(=O)[O-])S(=O)(=O)[O-].[Na+]

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Clinical Trial Information

NCT Number Recruitment interventions Conditions Sponsor/Collaborators Start Date Phases
NCT02507167 Completed Dietary Supplement: mixed meal|Drug: Rifampin Gastrointestinal Hormones University Medicine Greifswald November 2012 Phase 1

Tech Support

Answers to questions you may have can be found in the inhibitor handling instructions. Topics include how to prepare stock solutions, how to store inhibitors, and issues that need special attention for cell-based assays and animal experiments.

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Cell Lines Assay Type Concentration Incubation Time Formulation Activity Description PMID