Thiazolyl Blue

Catalog No.S6821 Batch:S682101

Technical Data

Formula

C₁₈H₁₆BrN₅S

Molecular Weight

414.32

CAS No.

298-93-1

Solubility (25°C)*

In vitro

DMSO

21 mg/mL (50.68 mM)

Ethanol

7 mg/mL (16.89 mM)

Water

2 mg/mL (4.82 mM)

In vivo (Add solvents to the product individually and in order)

Homogeneous suspension

CMC-NA

≥5mg/ml

Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

* <1 mg/ml means slightly soluble or insoluble.
* Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations.
* Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description	Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. This compound is reduced from yellow color to purple formazan in living cells.
In vitro	1.Preparation of MTT working solution Dissolve this compound by PBS to obtain 5 mg/mL of MTT. 2.Cell proliferation detection ( 96-well plates) 2.1Inoculated cells:Prepared single cell suspension to prepared with culture medium containing 10% FBS and inoculated into 96-well plate with 1000-10000 cells per well, volume of 100 uL per well. 2.2Incubated cells: 37℃. 5% CO2, Incubate for 24-72 h. 2.3Add 10 μL MTT in per well and incubate for 4 h, Discard the supernatant, For suspended cells, need centrifuge firstly. 2.4Add 100 μL DMSO, shock 10 min to dissolve the crystal completely 2.5Monitor the absorbance increase with an absorbance plate reader at OD = 562 nm.

Description

Thiazolyl Blue (MTT, Tetrazolium bromide, Methylthiazolyldiphenyl-tetrazolium bromide, Methylthialazole Tetrazolium) is a cell-permeable and positively charged colorimetric agent that is widely used to detect reductive metabolism in cells and to measure cell proliferation, cytotoxicity, and apoptosis. This compound is reduced from yellow color to purple formazan in living cells.

In vitro

1.Preparation of MTT working solution
Dissolve this compound by PBS to obtain 5 mg/mL of MTT.
2.Cell proliferation detection ( 96-well plates)
2.1Inoculated cells:Prepared single cell suspension to prepared with culture medium containing 10% FBS and inoculated into 96-well plate with 1000-10000 cells per well, volume of 100 uL per well.
2.2Incubated cells: 37℃. 5% CO2, Incubate for 24-72 h.
2.3Add 10 μL MTT in per well and incubate for 4 h, Discard the supernatant, For suspended cells, need centrifuge firstly.
2.4Add 100 μL DMSO, shock 10 min to dissolve the crystal completely
2.5Monitor the absorbance increase with an absorbance plate reader at OD = 562 nm.

Protocol (from reference)

References

https://pubmed.ncbi.nlm.nih.gov/16216776/

Selleck's Thiazolyl Blue Has Been Cited by 16 Publications

Exosomes displaying native EGF enhance doxorubicins therapeutic efficacy and reduce cardiotoxicity [ Journal of Nanobiotechnology, 2026 Jan 27, Article number: 66]	PubMed: 41593600
KSQ-4279, an Inhibitor of Ubiquitin Specific Peptidase 1, Enhanced the Chemotherapeutic Efficacy in ABCB1/ABCG2/ABCC1-Mediated Multidrug Resistant Cancers [ MedComm, 2025 Nov 29, e70517]	PubMed: 41328326
LRPPRC-Driven Oxidative Phosphorylation Is Associated with Elesclomol-Induced Cuproptosis in Ovarian Cancer [ International Journal of Molecular Sciences, 2025 Dec 31, 451]	PubMed: 41516324
Protective effect of norepinephrine, dopamine and N-methyldopamine against chemically-induced oxidative ferroptosis in HT22 neuronal cells: Protein disulfide isomerase as a mechanistic target for protection [ Free Radic Biol Med, 2025, 237:631-649]	PubMed: 40456499
LRPPRC-Driven Oxidative Phosphorylation Is Associated with Elesclomol-Induced Cuproptosis in Ovarian Cancer [ International Journal of Molecular Sciences, 2025, 451]	PubMed: 41516324
Sodium formate-induced mitochondrial impairment and cytotoxicity in neuronal cells reveal crucial pathogenic mechanisms underlying diabetic neuropathy and retinopathy [ Sci Rep, 2025, 15(1):27797]	PubMed: 40739109
N-Methyldopamine and ibopamine can prevent chemically-induced oxidative ferroptosis in vitro and in vivo [ J Pharmacol Exp Ther, 2025, 392(7):103620]	PubMed: 40570548
Stress granules affect the dual PI3K/mTOR inhibitor response by regulating the mitochondrial unfolded protein response [ Cancer Cell International, 2024 Jan 18, 38]	PubMed: 38238825
Stress granules affect the dual PI3K/mTOR inhibitor response by regulating the mitochondrial unfolded protein response [ Cancer Cell Int, 2024, 24(1):38]	PubMed: 38238825
Terazosin attenuates abdominal aortic aneurysm formation by downregulating Peg3 expression to inhibit vascular smooth muscle cell apoptosis and senescence [ Eur J Pharmacol, 2024, 968:176397]	PubMed: 38331337

RETURN POLICY
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SHIPPING AND STORAGE
Selleck products are transported at room temperature. If you receive the product at room temperature, please rest assured, the Selleck Quality Inspection Department has conducted experiments to verify that the normal temperature placement of one month will not affect the biological activity of powder products. After collecting, please store the product according to the requirements described in the datasheet. Most Selleck products are stable under the recommended conditions.

NOT FOR HUMAN, VETERINARY DIAGNOSTIC OR THERAPEUTIC USE.