Name: Seclidemstat (SP-2577)
Brand: Selleck Chemicals
SKU: S6722
Availability: InStock
Rating: 5 (3 reviews)

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Quality Control

Batch: Purity: 99.72%

99.72

Related Targets	PRMT EZH2 MAT2A DNA Methyltransferase JMJD G9a/GLP MLL NSD FTO SETD
Other LSD1 Products	Bomedemstat (IMG-7289) Vafidemstat GSK-2879552 INCB059872 dihydrochloride Pulrodemstat (CC-90011) besylate KDM1/LSD1 (Nuclear Marker) Antibody [P12K1] LSD1 Antibody [D13J4] T-3775440 HCl

Solubility

In vitro
Batch:

DMSO : 90 mg/mL (199.58 mM)
(Moisture-contaminated DMSO may reduce solubility. Use fresh, anhydrous DMSO.)

Water : Insoluble

Ethanol : Insoluble

Molarity Calculator

Mass	Concentration	Volume	Molecular Weight
Mass value Mass unit	Concentration value Concentration unit	Volume value Volume unit	Molecular weight (g/mol)

Dilution Calculator Molecular Weight Calculator

In vivo batch selection In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	5.000mg/ml (11.09mM)	Taking the 1 mL working solution as an example, add 50 μL of 100 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 Corn oil Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	4.500mg/ml (9.98mM)	Taking the 1 mL working solution as an example, add 50 μL of 90 mg/ml clear DMSO stock solution to 950 μL of corn oil and mix evenly. The mixed solution should be used immediately for optimal results.
Clear solution, mainly for i.p.	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Validated by Selleck labs. Should you need adjustments to this formulation, contact our sales team for custom testing.	8.000mg/ml (17.74mM)	Taking the 1 mL working solution as an example, add 50 μL of 160 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo Formulation Calculator (Clear solution)

Step 1: Enter information below (Recommended: An additional animal making an allowance for loss during the experiment)

Dosage: mg/kg

Average weight of animals: g

Dosing volume per animal: μL

Number of animals:

Step 2: Enter the in vivo formulation (This is only the calculator, not formulation. Please contact us first if there is no in vivo formulation at the solubility Section.)

% DMSO

%

% Tween 80

% ddH₂O

% DMSO

+

%

Calculation results:

Working concentration: mg/ml;

Method for preparing DMSO master liquid: mg drug pre-dissolved in μL DMSO ( Master liquid concentration mg/mL, Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug. )

Method for preparing in vivo formulation: Take μL DMSO master liquid, next addμL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH₂O, mix and clarify.

Method for preparing in vivo formulation: Take μL DMSO master liquid, next add μL Corn oil, mix and clarify.

Note: 1. Please make sure the liquid is clear before adding the next solvent.
2. Be sure to add the solvent(s) in order. You must ensure that the solution obtained, in the previous addition, is a clear solution before proceeding to add the next solvent. Physical methods such
as vortex, ultrasound or hot water bath can be used to aid dissolving.

Chemical Information, Storage & Stability

Molecular Weight	450.94	Formula	C₂₀H₂₃ClN₄O₄S	Storage (From the date of receipt)	3 years -20°C powder
CAS No.	1423715-37-0	--		Storage of Stock Solutions	1 year-80°Cin solvent 1 month-20°Cin solvent
Synonyms	SP-2577 mesylate			Smiles	CC(=NNC(=O)C1=CC(=CC=C1)S(=O)(=O)N2CCN(CC2)C)C3=C(C=CC(=C3)Cl)O

Mechanism of Action

Targets/IC₅₀/Ki	LSD1/KDM1A (Cell-free assay) 13 nM
In vitro	The combination therapy of Seclidemstat (SP-2577) with checkpoint inhibitors may induce or augment immunogenic responses of SWI/SNF-mutated ovarian cancers. This compound promotes endogenous retroviruses (ERVs) expression and activation of IFNβ pathway in small cell carcinoma of the ovary hypercalcemic type (SCCOHT) cell lines. It (3 μM) promotes PD-L1 expression in SCCOHT COV 434 pIND 20 BRG1-2.7 cell line.
In vivo	SP-2577 (Seclidemstat) is an inhibitor of lysine-specific demthylase KDM1A (LSD1) potential antineoplastic activity against pediatric sarcomas.
References	[1] https://pubmed.ncbi.nlm.nih.gov/32649682/ [2] https://patents.google.com/patent/WO2017004519A1/en [3] https://pubmed.ncbi.nlm.nih.gov/34453478/

Clinical Trial Information

(data from https://clinicaltrials.gov, updated on 2024-05-22)

NCT Number	Recruitment	Conditions	Sponsor/Collaborators	Start Date	Phases
NCT04611139	Withdrawn	SCCOHT\|Ovarian Clear Cell Tumor\|Ovarian Endometrioid Adenocarcinoma\|Endometrial Cancer	HonorHealth Research Institute\|Merck Sharp & Dohme LLC\|Salarius Pharmaceuticals LLC	December 31 2021	Phase 1
NCT03895684	Completed	Advanced Solid Tumors	Salarius Pharmaceuticals LLC	June 25 2019	Phase 1
NCT03600649	Active not recruiting	Ewing Sarcoma\|Myxoid Liposarcoma\|SarcomaSoft Tissue\|Desmoplastic Small Round Cell Tumor\|Extraskeletal Myxoid Chondrosarcoma\|Angiomatoid Fibrous Histiocytoma\|Clear Cell Sarcoma\|Primary Pulmonary Myxoid Sarcoma\|Myoepithelial Tumor\|Sclerosing Epithelioid Fibrosarcoma\|Fibromyxoid Tumor	Salarius Pharmaceuticals LLC\|National Pediatric Cancer Foundation	June 4 2018	Phase 1

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Handling Instructions

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Seclidemstat (SP-2577) LSD1 inhibitor

Chemical Structure

Molecular Weight: 450.94