Seclidemstat (SP-2577)

Catalog No.S6722 Batch:S672202

Technical Data

Formula	C₂₀H₂₃ClN₄O₄S
Molecular Weight	450.94	CAS No.	1423715-37-0
Solubility (25°C)*	In vitro	DMSO	90 mg/mL (199.58 mM)
		Water	Insoluble
		Ethanol	Insoluble
* <1 mg/ml means slightly soluble or insoluble. * Please note that Selleck tests the solubility of all compounds in-house, and the actual solubility may differ slightly from published values. This is normal and is due to slight batch-to-batch variations. * Room temperature shipping (Stability testing shows this product can be shipped without any cooling measures.)

Preparing Stock Solutions

Biological Activity

Description

Seclidemstat (SP-2577) is a potent and orally bioavailable inhibitor of lysine-specific demethylase 1 (LSD1/KDM1A) with IC50 of 13 nM. Seclidemstat (SP-2577) has potential antineoplastic activity.

Targets

LSD1/KDM1A ^[1]
(Cell-free assay)

13 nM

In vitro

The combination therapy of Seclidemstat (SP-2577) with checkpoint inhibitors may induce or augment immunogenic responses of SWI/SNF-mutated ovarian cancers. Seclidemstat promotes endogenous retroviruses (ERVs) expression and activation of IFNβ pathway in small cell carcinoma of the ovary hypercalcemic type (SCCOHT) cell lines. Seclidemstat (3 μM) promotes PD-L1 expression in SCCOHT COV 434 pIND 20 BRG1-2.7 cell line.^[1]

In vivo

SP-2577 (Seclidemstat) is an inhibitor of lysine-specific demthylase KDM1A (LSD1) potential antineoplastic activity against pediatric sarcomas.

Protocol (from reference)

Cell Assay:^{^[1]}	Cell lines Small cell carcinoma of the ovary hypercalcemic type (SCCOHT) cell lines, ovarian clear cell carcinomas (OCCC) cell lines Concentrations 1μM,3 μM Incubation Time 72 h Method The SCCOHT and OCCC cell lines are seeded in T-25 tissue culture treated flasks at 3 x 10₅ cells in 5mL of the appropriate phenol red free complete growth medium. After 48 h, at 70–80% confluency, the cells are treated with 3μM or 1μM of Seclidemstat. After 72 h, the media is collected from the flasks and stored at -80°C until use for various assays.
Animal Study:^{^[3]}	Animal Models C.B.17SC scid−/− female mice Dosages 100 mg/kg Administration i.p.

Cell Assay:^{^[1]}

Cell lines
Small cell carcinoma of the ovary hypercalcemic type (SCCOHT) cell lines, ovarian clear cell carcinomas (OCCC) cell lines
Concentrations
1μM,3 μM
Incubation Time
72 h
Method
The SCCOHT and OCCC cell lines are seeded in T-25 tissue culture treated flasks at 3 x 10₅ cells in 5mL of the appropriate phenol red free complete growth medium. After 48 h, at 70–80% confluency, the cells are treated with 3μM or 1μM of Seclidemstat. After 72 h, the media is collected from the flasks and stored at -80°C until use for various assays.

Animal Study:^{^[3]}

Animal Models
C.B.17SC scid−/− female mice
Dosages
100 mg/kg
Administration
i.p.

References

Selleck's Seclidemstat (SP-2577) has been cited by 1 publication

Repression of LSD1 potentiates homologous recombination-proficient ovarian cancer to PARP inhibitors through down-regulation of BRCA1/2 and RAD51 [ Nat Commun, 2023, 10.1038/s41467-023-42850-x]	PubMed: 37973845

Repression of LSD1 potentiates homologous recombination-proficient ovarian cancer to PARP inhibitors through down-regulation of BRCA1/2 and RAD51 [ Nat Commun, 2023, 10.1038/s41467-023-42850-x]

PubMed: 37973845

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SHIPPING AND STORAGE
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